pycom04g11270

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0006939
Physical Location & Seq
Reverse (-)
14144786 .. 14148888
4103 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1725 bp
ATGGAAGCTGCAAGTGAGGCAGGGAACGCCGATGGGCTCGAAGCTATGTGCATGGAAGACTCCTCTTGGCACCCATGCCAAGTTTCTCTCAGCTCTACTGGAGATAGCCTAATTGTAGAATTTGGGCGCCAGGAGTTAGAAGATATGGTGTTGAACAAGGATGAAGCACTTTCCCGTCTCCGCTTCCGTTCTACTCCTCTTGAAGGCAATGACTGCGCTCACATTGAAGGAGAGAAAGTTCTTGCAATTAACAAGTCACAGTTTAAGAGCCACTTATGTGATGCCAAGGTGGAAAAGGTGCTCAGAGTGAGACACTCACAGAGGGTCTATTGTAGATGCACTTTCATGATCGAATGGCTTCACCAAGAACTCAAGGGACAGACCGTGACTCTCCCCTCTAGTTCAATTATGAAACTAGCAAGCAAAAACATCATTGTCCATCCAACTGTTGCTGCATTCTTGAAATCAGTTAAACAAATGGGTCCGAATAGTGCTTCTCCCTTTCCAGTCGTGCATGAGGAGTTTGGATTTGAATTGGACCTTAACAAATTGCTGGAAAAGCAAATAGAAGAGATCAATATTTCAACTAATGGATTCAGAAAAGAAATCACAGAAAACATTTTAGAGGGGGTCAAAGATGCTCAGGTTTCTCATGACCAAGATCAATCAAAAAGTACTGCTCAGAGTTCTATTGAATACGAAGTAAACATAGAAGATGAGGGTCTACAAGCCCCTCGCTCATCCATGCAAGAAGAGCATTCAGAATACAAGTCTCGCCTTAGTCCTCTTGGTGCTCGTGCAGCTCTGGCCTTACTAGTATCAAGTAGTCACAAGCATATTGCTATAGGTGGGACTGAACTTTTCAAATCTTGTGATAGTGACAACATGTTTACTAAAGATACTAAAGCTCATAAGAATGAAAATAAGACTTCAGATGCTACAAAAACGAGCTCAGTTGTGCAGAGAGGTTTTGAAATTCAAAATAGTGATACTCACCTGAGAAATTCTGCCGAAGAGATTAAGCCAAGGGCCTTAACCAGTGCAAGAAGGTTGACACGCTCAGCAATCCAACAAGAAAAATATAAATTGACTGTGACAGTTGAACAAGGGTTGGACGAGAGCAAGTCAGCTCATGCTGCAGGGTCATTTTCTTCAGTAGGAAATGTCACCATTCATGAAAGCAATGTATTGAAAGAGAAGAATGATGTATCAAAAAAGAAGAAAGTAGTCTCTTCGCCATTTCATGCCGAGAGTAACATTCCTATGGAAGAAAGAGATAAAAAGCTGATGTCTGGTGATGCTGGAGCCATTCAAGATATATGTGATCCAATTAAGACTTGTGGTAAAGAGTCTAAAAACACTAAGTCAGCTGTTACAACCAACATGAGGAGGTCGACTCGTTTGACATGTTCTTCTGAAGAGAATGGAACAGTTCCTGAAAACCATGGAAAGGAAGAGAAAAGTGGTGGACCAAGAAAGAAAACAGCTGTCTCTCCTTCAGCTGGCAAGGACAAGAACATGCATGGCGAAGAAAGCAATGAAAAGCACATGTCTGGTGCTGTCACAAGATCTGGAAACACTCGACAAATTGAAGGAAAAGTTTCAGGAAGTGGTGGTAAAAGCCAAGGCCAGAAGAGGAAGTCTGCTTCCTCCAGTAAGCAAGAGCTGCGATTTTCTCCACGATTGAAGTTTCTCCCTCAGACTCGCTCACAAAAGAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

575

Amino Acids

63.24

Weight (kDa)

8.6

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 13 - 136 1.5e-21 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 69, 126
AccI GTMKAC 2 cut(s) 724, 1394
AciI CCGC 1 cut(s) 181
AclWI GGATC 1 cut(s) 1319
AcsI RAATTY 3 cut(s) 119, 975, 1003
AcuI CTGAAG 4 cut(s) 915, 1137, 1437, 1482
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 4 cut(s) 203, 1386, 1450, 1502
AflIII ACRYGT 3 cut(s) 885, 1406, 1548
AhlI ACTAGT 1 cut(s) 814
AjnI CCWGG 1 cut(s) 129
AleI CACNNNNGTG 1 cut(s) 276
AloI GAACNNNNNNTCC 2 cut(s) 222, 254
Alw21I GWGCWC 3 cut(s) 303, 796, 953
Alw26I GTCTC 5 cut(s) 182, 304, 777, 1234, 1495
AlwI GGATC 1 cut(s) 1319
AlwNI CAGNNNCTG 1 cut(s) 1436
AoxI GGCC 3 cut(s) 807, 1029, 1627
ApeKI GCWGC 5 cut(s) 8, 452, 800, 1136, 1666
ApoI RAATTY 3 cut(s) 119, 975, 1003
Asp700I GAANNNNTTC 2 cut(s) 357, 1600
AspLEI GCGC 2 cut(s) 129, 218
AspS9I GGNCC 4 cut(s) 482, 538, 1029, 1469
AsuHPI GGTGA 4 cut(s) 353, 986, 1159, 1307
AvaII GGWCC 3 cut(s) 482, 538, 1469
BanI GGYRCC 2 cut(s) 69, 126
BanII GRGCYC 2 cut(s) 39, 953
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 3 cut(s) 303, 796, 953
BbvI GCAGC 4 cut(s) 439, 812, 1123, 1653
BccI CCATC 2 cut(s) 26, 447
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 5 cut(s) 182, 304, 777, 1234, 1495
BcuI ACTAGT 1 cut(s) 814
BfaI CTAG 3 cut(s) 399, 416, 815
BfmI CTRYAG 2 cut(s) 843, 1137
BfoI RGCGCY 1 cut(s) 130
BglII AGATCT 1 cut(s) 1568
BisI GCNGC 5 cut(s) 9, 453, 801, 1137, 1667
BlpI GCTNAGC 1 cut(s) 1060
BlsI GCNGC 5 cut(s) 10, 454, 802, 1138, 1668
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 3 cut(s) 482, 538, 1469
BmgT120I GGNCC 4 cut(s) 482, 538, 1029, 1469
BmiI GGNNCC 4 cut(s) 71, 128, 483, 1306
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 5 cut(s) 271, 326, 628, 925, 1288
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 1381, 1413
BpmI CTGGAG 3 cut(s) 120, 1323, 1636
Bpu10I CCTNAGC 1 cut(s) 642
Bpu1102I GCTNAGC 1 cut(s) 1060
BpuEI CTTGAG 1 cut(s) 356
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 4 cut(s) 285, 1025, 1444, 1624
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 1386, 1450, 1502
Bse1I ACTGG 4 cut(s) 103, 506, 1038, 1653
Bse3DI GCAATG 3 cut(s) 214, 1189, 1543
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 4 cut(s) 285, 1025, 1444, 1624
BseGI GGATG 3 cut(s) 166, 439, 740
BseLI CCNNNNNNNGG 4 cut(s) 203, 1386, 1450, 1502
BseMI GCAATG 3 cut(s) 214, 1189, 1543
BseMII CTCAG 8 cut(s) 103, 316, 656, 695, 966, 989, 1074, 1712
BseNI ACTGG 4 cut(s) 103, 506, 1038, 1653
BseRI GAGGAG 4 cut(s) 52, 186, 533, 1402
BseXI GCAGC 4 cut(s) 439, 812, 1123, 1653
BsgI GTGCAG 2 cut(s) 819, 980
BshFI GGCC 3 cut(s) 809, 1031, 1629
BshNI GGYRCC 2 cut(s) 69, 126
BsiHKAI GWGCWC 3 cut(s) 303, 796, 953
BslFI GGGAC 2 cut(s) 390, 865
BslI CCNNNNNNNGG 4 cut(s) 203, 1386, 1450, 1502
BsmAI GTCTC 5 cut(s) 182, 304, 777, 1234, 1495
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 390, 865
BsmI GAATGC 2 cut(s) 455, 757
BsnI GGCC 3 cut(s) 809, 1031, 1629
Bsp1286I GDGCHC 4 cut(s) 39, 303, 796, 953
Bsp143I GATC 5 cut(s) 348, 573, 661, 1324, 1568
Bsp1720I GCTNAGC 1 cut(s) 1060
Bsp19I CCATGG 1 cut(s) 1444
BspACI CCGC 1 cut(s) 181
BspANI GGCC 3 cut(s) 809, 1031, 1629
BspCNI CTCAG 8 cut(s) 102, 315, 655, 694, 965, 990, 1073, 1711
BspHI TCATGA 3 cut(s) 345, 652, 1174
BspLI GGNNCC 4 cut(s) 71, 128, 483, 1306
BspMAI CTGCAG 1 cut(s) 1141
BspPI GGATC 1 cut(s) 1319
BspQI GCTCTTC 1 cut(s) 747
BspT107I GGYRCC 2 cut(s) 69, 126
BsrDI GCAATG 3 cut(s) 214, 1189, 1543
BsrI ACTGG 4 cut(s) 103, 506, 1038, 1653
BssECI CCNNGG 4 cut(s) 285, 1025, 1444, 1624
BssMI GATC 5 cut(s) 348, 573, 661, 1324, 1568
BssNI GRCGYC 1 cut(s) 127
BssSI CACGAG 1 cut(s) 795
BssT1I CCWWGG 4 cut(s) 285, 1025, 1444, 1624
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 6 cut(s) 261, 385, 448, 1093, 1099, 1432
Bst6I CTCTTC 7 cut(s) 564, 747, 1008, 1237, 1413, 1449, 1628
BstACI GRCGYC 1 cut(s) 127
BstAPI GCANNNNNTGC 2 cut(s) 213, 1666
BstC8I GCNNGC 2 cut(s) 421, 1504
BstDSI CCRYGG 1 cut(s) 1444
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 166, 439, 740
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 218
BstKTI GATC 5 cut(s) 351, 576, 664, 1327, 1571
BstMAI GTCTC 5 cut(s) 182, 304, 777, 1234, 1495
BstMBI GATC 5 cut(s) 348, 573, 661, 1324, 1568
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 4 cut(s) 889, 1410, 1522, 1552
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 2 cut(s) 843, 1137
BstV1I GCAGC 4 cut(s) 439, 812, 1123, 1653
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 1568
BstYI RGATCY 1 cut(s) 1568
BsuRI GGCC 3 cut(s) 809, 1031, 1629
BtgI CCRYGG 1 cut(s) 1444
BtsCI GGATG 3 cut(s) 166, 439, 740
BtsIMutI CAGTG 1 cut(s) 1045
Cac8I GCNNGC 2 cut(s) 421, 1504
CaiI CAGNNNCTG 1 cut(s) 1436
CciI TCATGA 3 cut(s) 345, 652, 1174
CfoI GCGC 2 cut(s) 129, 218
Cfr13I GGNCC 4 cut(s) 482, 538, 1029, 1469
Csp6I GTAC 1 cut(s) 675
CviQI GTAC 1 cut(s) 675
DinI GGCGCC 1 cut(s) 128
DpnI GATC 5 cut(s) 350, 575, 663, 1326, 1570
DpnII GATC 5 cut(s) 348, 573, 661, 1324, 1568
Eam1104I CTCTTC 7 cut(s) 564, 747, 1008, 1237, 1413, 1449, 1628
EarI CTCTTC 7 cut(s) 564, 747, 1008, 1237, 1413, 1449, 1628
Ecl136II GAGCTC 1 cut(s) 951
Eco130I CCWWGG 4 cut(s) 285, 1025, 1444, 1624
Eco24I GRGCYC 2 cut(s) 39, 953
Eco47I GGWCC 3 cut(s) 482, 538, 1469
Eco53kI GAGCTC 1 cut(s) 951
Eco57I CTGAAG 4 cut(s) 915, 1137, 1437, 1482
EcoICRI GAGCTC 1 cut(s) 951
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 1029
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 4 cut(s) 285, 1025, 1444, 1624
EcoT22I ATGCAT 1 cut(s) 1524
EcoT38I GRGCYC 2 cut(s) 39, 953
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 4 cut(s) 285, 1025, 1444, 1624
Esp3I CGTCTC 1 cut(s) 182
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 2 cut(s) 390, 865
FblI GTMKAC 2 cut(s) 724, 1394
Fnu4HI GCNGC 5 cut(s) 9, 453, 801, 1137, 1667
FokI GGATG 3 cut(s) 173, 426, 727
FriOI GRGCYC 2 cut(s) 39, 953
Fsp4HI GCNGC 5 cut(s) 9, 453, 801, 1137, 1667
FspBI CTAG 3 cut(s) 399, 416, 815
GlaI GCGC 2 cut(s) 128, 217
GluI GCNGC 5 cut(s) 9, 453, 801, 1137, 1667
GsuI CTGGAG 3 cut(s) 120, 1323, 1636
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 809, 1031, 1629
HhaI GCGC 2 cut(s) 129, 218
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 2 cut(s) 127, 216
HinP1I GCGC 2 cut(s) 127, 216
HincII GTYRAC 2 cut(s) 1053, 1395
HindII GTYRAC 2 cut(s) 1053, 1395
HinfI GANTC 6 cut(s) 59, 388, 594, 1349, 1396, 1702
HphI GGTGA 4 cut(s) 353, 986, 1159, 1307
Hpy166II GTNNAC 6 cut(s) 706, 725, 891, 1053, 1395, 1469
Hpy188I TCNGA 8 cut(s) 305, 486, 599, 684, 763, 934, 1417, 1701
Hpy188III TCNNGA 9 cut(s) 200, 346, 460, 653, 1175, 1313, 1436, 1572, 1605
Hpy8I GTNNAC 6 cut(s) 706, 725, 891, 1053, 1395, 1469
HpyAV CCTTC 5 cut(s) 197, 221, 1041, 1506, 1586
HpyCH4III ACNGT 6 cut(s) 261, 385, 448, 1093, 1099, 1432
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 2 cut(s) 127, 216
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 5 cut(s) 348, 573, 661, 1324, 1568
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 1 cut(s) 1304
Lsp1109I GCAGC 4 cut(s) 439, 812, 1123, 1653
LweI GCATC 5 cut(s) 271, 326, 628, 925, 1288
MaeI CTAG 3 cut(s) 399, 416, 815
MaeIII GTNAC 9 cut(s) 255, 385, 827, 878, 1093, 1165, 1253, 1372, 1561
MalI GATC 5 cut(s) 350, 575, 663, 1326, 1570
MboI GATC 5 cut(s) 348, 573, 661, 1324, 1568
MflI RGATCY 1 cut(s) 1568
MhlI GDGCHC 4 cut(s) 39, 303, 796, 953
Mly113I GGCGCC 1 cut(s) 127
MlyI GAGTC 5 cut(s) 53, 382, 1358, 1390, 1696
MmeI TCCRAC 3 cut(s) 467, 1092, 1093
Mph1103I ATGCAT 1 cut(s) 1524
MroXI GAANNNNTTC 2 cut(s) 357, 1600
MseI TTAA 8 cut(s) 249, 264, 471, 543, 1020, 1034, 1332, 1723
MslI CAYNNNNRTG 4 cut(s) 276, 344, 915, 1262
MspA1I CMGCKG 3 cut(s) 1370, 1487, 1502
MspR9I CCNGG 1 cut(s) 131
Mva1269I GAATGC 2 cut(s) 455, 757
MvaI CCWGG 1 cut(s) 131
NarI GGCGCC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 1444
NdeII GATC 5 cut(s) 348, 573, 661, 1324, 1568
NlaIV GGNNCC 4 cut(s) 71, 128, 483, 1306
NmeAIII GCCGAG 1 cut(s) 1273
NmuCI GTSAC 7 cut(s) 255, 385, 827, 878, 1093, 1165, 1561
NsiI ATGCAT 1 cut(s) 1524
NspI RCATGY 4 cut(s) 889, 1410, 1522, 1552
OliI CACNNNNGTG 1 cut(s) 276
PagI TCATGA 3 cut(s) 345, 652, 1174
PciI ACATGT 3 cut(s) 885, 1406, 1548
PciSI GCTCTTC 1 cut(s) 747
PctI GAATGC 2 cut(s) 455, 757
PdmI GAANNNNTTC 2 cut(s) 357, 1600
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 5 cut(s) 10, 454, 802, 1138, 1668
PleI GAGTC 5 cut(s) 53, 382, 1357, 1390, 1696
PluTI GGCGCC 1 cut(s) 130
PpsI GAGTC 5 cut(s) 53, 382, 1357, 1390, 1696
PscI ACATGT 3 cut(s) 885, 1406, 1548
Psp124BI GAGCTC 1 cut(s) 953
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 4 cut(s) 71, 128, 483, 1306
PspPI GGNCC 4 cut(s) 482, 538, 1029, 1469
PstI CTGCAG 1 cut(s) 1141
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 1 cut(s) 1568
PvuII CAGCTG 3 cut(s) 1370, 1487, 1502
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 4 cut(s) 276, 344, 915, 1262
SacI GAGCTC 1 cut(s) 953
SalI GTCGAC 1 cut(s) 1393
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 8 cut(s) 249, 264, 471, 543, 1020, 1034, 1332, 1723
SatI GCNGC 5 cut(s) 9, 453, 801, 1137, 1667
Sau3AI GATC 5 cut(s) 348, 573, 661, 1324, 1568
Sau96I GGNCC 4 cut(s) 482, 538, 1029, 1469
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 5 cut(s) 53, 382, 1358, 1390, 1696
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 4 cut(s) 39, 303, 796, 953
SfaNI GCATC 5 cut(s) 271, 326, 628, 925, 1288
SfcI CTRYAG 2 cut(s) 843, 1137
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 3 cut(s) 482, 538, 1469
SmiMI CAYNNNNRTG 4 cut(s) 276, 344, 915, 1262
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 814
SsiI CCGC 1 cut(s) 181
SspDI GGCGCC 1 cut(s) 126
SspI AATATT 1 cut(s) 580
SspMI CTAG 3 cut(s) 399, 416, 815
SstI GAGCTC 1 cut(s) 953
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 4 cut(s) 285, 1025, 1444, 1624
TaaI ACNGT 6 cut(s) 261, 385, 448, 1093, 1099, 1432
TaqI TCGA 4 cut(s) 39, 351, 1394, 1582
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 8 cut(s) 249, 264, 471, 543, 1020, 1034, 1332, 1723
Tru9I TTAA 8 cut(s) 249, 264, 471, 543, 1020, 1034, 1332, 1723
TscAI CASTG 1 cut(s) 1045
TseFI GTSAC 7 cut(s) 255, 385, 827, 878, 1093, 1165, 1561
TseI GCWGC 5 cut(s) 8, 452, 800, 1136, 1666
Tsp45I GTSAC 7 cut(s) 255, 385, 827, 878, 1093, 1165, 1561
TspDTI ATGAA 8 cut(s) 177, 334, 425, 933, 1163, 1191, 1232, 1554
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 1 cut(s) 1045
VpaK11BI GGWCC 3 cut(s) 482, 538, 1469
XagI CCTNNNNNAGG 1 cut(s) 201
XapI RAATTY 3 cut(s) 119, 975, 1003
XceI RCATGY 4 cut(s) 889, 1410, 1522, 1552
XmiI GTMKAC 2 cut(s) 724, 1394
XmnI GAANNNNTTC 2 cut(s) 357, 1600
XspI CTAG 3 cut(s) 399, 416, 815
ZrmI AGTACT 1 cut(s) 676
Zsp2I ATGCAT 1 cut(s) 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.