pycom04g18600

39S ribosomal protein L45

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013393
Physical Location & Seq
Forward (+)
20577606 .. 20580654
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGGCTTTCAGAAGCTTACACCAGATTGGAGCTCTCTATCGTACAGCCCAGATCGCAGAATCCTCCCATCTGCTTGGAAGCTCAAGAAACTATGCAACTGGTATTTCAAATGTCCCGGAATCTTCCTTGGAGTCAGGGAGTTCCATGACTTTGCGTTCTGCAATGGCTGTTGAGTTGCCGCTTTTCTCGAGTGACACAAGGTCGCTATCAACACAAGTAAAAGCCCCTGCACAAGCAAGACAAATGATCATACGTATGAACATTCTGATGTTTTTATGCATCAGATGGTTCACAAGAAGGGGGTGGAAAAGAACAAAAGAAGACACAGTTATAGAGCTCAAAAGTGCATATGCCATTTCTAAATTAATAAAAAAGGGGTATTCAAAGAATCTATTCTACAAAGAAGCAATAAACTTATACATGCAGATAAATACTCTAATGGCAAATGGGGACAAAAATTCTTTAAGGAAAGCAGTTACAGAGAGAATGTTTTTGGAACTCAAGAATGAAATAAAACAAAGAGACTCAATATCGAGTAAGGTTTTCTGGGAAATGGTGGAACCGGTTGTAAAAATACAAACTTTGAGAGCACGACTGATTGGTGTCAATCGAAATGATCTTGAGAAGGTGTTCATACAGCTTACGCTCGAGTTTCTGACAAAGCAGAAATTCGAAGCATATGACTCGAAGGGTGCTGTTGTTGCTGGAGATAAATCGAAGGAGGTAAAGTGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

246

Amino Acids

27.92

Weight (kDa)

9.86

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim44 PF04280 115 - 244 1.1e-14 Tim44-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 179
AcsI RAATTY 2 cut(s) 457, 668
AfaI GTAC 1 cut(s) 43
AgeI ACCGGT 1 cut(s) 562
AgsI TTSAA 2 cut(s) 108, 384
AhdI GACNNNNNGTC 1 cut(s) 199
AluBI AGCT 5 cut(s) 15, 32, 81, 337, 640
AluI AGCT 5 cut(s) 15, 32, 81, 337, 640
Alw21I GWGCWC 3 cut(s) 34, 339, 592
Alw26I GTCTC 1 cut(s) 516
Ama87I CYCGRG 2 cut(s) 187, 647
ApoI RAATTY 2 cut(s) 457, 668
AseI ATTAAT 1 cut(s) 365
AsiGI ACCGGT 1 cut(s) 562
Asp700I GAANNNNTTC 2 cut(s) 392, 629
AsuC2I CCSGG 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 672
AvaI CYCGRG 2 cut(s) 187, 647
BanII GRGCYC 2 cut(s) 34, 339
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 3 cut(s) 34, 339, 592
BccI CCATC 2 cut(s) 75, 279
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 516
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 116
BmeRI GACNNNNNGTC 1 cut(s) 199
BmeT110I CYCGRG 2 cut(s) 187, 647
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 672
BpuEI CTTGAG 3 cut(s) 67, 485, 641
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 1 cut(s) 713
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 213
BshTI ACCGGT 1 cut(s) 562
BsiHKAI GWGCWC 3 cut(s) 34, 339, 592
BsiHKCI CYCGRG 2 cut(s) 187, 647
BsiSI CCGG 2 cut(s) 116, 563
BslFI GGGAC 2 cut(s) 98, 464
BsmAI GTCTC 1 cut(s) 516
BsmFI GGGAC 2 cut(s) 98, 464
BsoBI CYCGRG 2 cut(s) 187, 647
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 3 cut(s) 34, 339, 592
Bsp143I GATC 3 cut(s) 51, 246, 616
BspACI CCGC 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 561
BspT104I TTCGAA 1 cut(s) 672
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 3 cut(s) 51, 246, 616
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 328
BstBAI YACGTR 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 672
BstKTI GATC 3 cut(s) 54, 249, 619
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 3 cut(s) 51, 246, 616
BstMWI GCNNNNNNNGC 2 cut(s) 53, 701
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 114
BstSNI TACGTA 1 cut(s) 254
BstV2I GAAGAC 1 cut(s) 327
BstXI CCANNNNNNTGG 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 562
Csp6I GTAC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 562
CviAII CATG 2 cut(s) 145, 421
CviJI RGCY 9 cut(s) 5, 15, 32, 47, 81, 167, 224, 337, 640
CviKI_1 RGCY 9 cut(s) 5, 15, 32, 47, 81, 167, 224, 337, 640
CviQI GTAC 1 cut(s) 42
DpnI GATC 3 cut(s) 53, 248, 618
DpnII GATC 3 cut(s) 51, 246, 616
DriI GACNNNNNGTC 1 cut(s) 199
Eam1105I GACNNNNNGTC 1 cut(s) 199
Ecl136II GAGCTC 2 cut(s) 32, 337
Eco105I TACGTA 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 2 cut(s) 34, 339
Eco53kI GAGCTC 2 cut(s) 32, 337
Eco88I CYCGRG 2 cut(s) 187, 647
EcoICRI GAGCTC 2 cut(s) 32, 337
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 281
EcoT38I GRGCYC 2 cut(s) 34, 339
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 148, 424
FaqI GGGAC 2 cut(s) 98, 464
FatI CATG 2 cut(s) 144, 420
FauNDI CATATG 2 cut(s) 349, 679
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 34, 339
Fsp4HI GCNGC 1 cut(s) 179
GluI GCNGC 1 cut(s) 179
GsuI CTGGAG 1 cut(s) 726
HapII CCGG 2 cut(s) 116, 563
Hin1II CATG 2 cut(s) 148, 424
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 6 cut(s) 59, 119, 131, 388, 524, 683
HpaII CCGG 2 cut(s) 116, 563
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 4 cut(s) 11, 267, 284, 657
Hpy188III TCNNGA 4 cut(s) 84, 187, 502, 620
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 4 cut(s) 291, 619, 682, 712
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 6 cut(s) 95, 161, 230, 279, 347, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 701
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 2 cut(s) 148, 424
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 3 cut(s) 51, 246, 616
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 9 cut(s) 35, 62, 84, 120, 129, 240, 532, 576, 690
LweI GCATC 1 cut(s) 288
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 2 cut(s) 191, 475
MalI GATC 3 cut(s) 53, 248, 618
MboI GATC 3 cut(s) 51, 246, 616
MboII GAAGA 2 cut(s) 114, 332
MhlI GDGCHC 3 cut(s) 34, 339, 592
MluCI AATT 3 cut(s) 362, 457, 668
MlyI GAGTC 3 cut(s) 140, 518, 677
MnlI CCTC 2 cut(s) 73, 715
Mph1103I ATGCAT 1 cut(s) 281
MroXI GAANNNNTTC 2 cut(s) 392, 629
MseI TTAA 2 cut(s) 365, 464
MslI CAYNNNNRTG 2 cut(s) 254, 266
MspI CCGG 2 cut(s) 116, 563
MspR9I CCNGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 53, 701
NciI CCSGG 1 cut(s) 116
NdeI CATATG 2 cut(s) 349, 679
NdeII GATC 3 cut(s) 51, 246, 616
NlaIII CATG 2 cut(s) 148, 424
NlaIV GGNNCC 1 cut(s) 561
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 281
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 1 cut(s) 672
PaeR7I CTCGAG 2 cut(s) 187, 647
PdmI GAANNNNTTC 2 cut(s) 392, 629
PfeI GAWTC 3 cut(s) 59, 119, 388
PfoI TCCNGGA 1 cut(s) 114
PinAI ACCGGT 1 cut(s) 562
PkrI GCNGC 1 cut(s) 180
PleI GAGTC 3 cut(s) 139, 518, 677
PpsI GAGTC 3 cut(s) 139, 518, 677
Ppu21I YACGTR 1 cut(s) 254
PshBI ATTAAT 1 cut(s) 365
Psp124BI GAGCTC 2 cut(s) 34, 339
PspN4I GGNNCC 1 cut(s) 561
PspXI VCTCGAGB 1 cut(s) 647
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 2 cut(s) 254, 266
SacI GAGCTC 2 cut(s) 34, 339
SaqAI TTAA 2 cut(s) 365, 464
SatI GCNGC 1 cut(s) 179
Sau3AI GATC 3 cut(s) 51, 246, 616
SchI GAGTC 3 cut(s) 140, 518, 677
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 3 cut(s) 34, 339, 592
SfaNI GCATC 1 cut(s) 288
Sfr274I CTCGAG 2 cut(s) 187, 647
SfuI TTCGAA 1 cut(s) 672
SlaI CTCGAG 2 cut(s) 187, 647
SmiMI CAYNNNNRTG 2 cut(s) 254, 266
SmlI CTYRAG 5 cut(s) 82, 187, 500, 620, 647
SmoI CTYRAG 5 cut(s) 82, 187, 500, 620, 647
SnaBI TACGTA 1 cut(s) 254
Sse9I AATT 3 cut(s) 362, 457, 668
SsiI CCGC 1 cut(s) 179
SstI GAGCTC 2 cut(s) 34, 339
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 256
TaqI TCGA 7 cut(s) 188, 533, 610, 648, 672, 686, 716
TasI AATT 3 cut(s) 362, 457, 668
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 3 cut(s) 59, 119, 388
Tru1I TTAA 2 cut(s) 365, 464
Tru9I TTAA 2 cut(s) 365, 464
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 272, 522, 622
VspI ATTAAT 1 cut(s) 365
XapI RAATTY 2 cut(s) 457, 668
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 2 cut(s) 187, 647
XmnI GAANNNNTTC 2 cut(s) 392, 629
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.