pycom04g20020

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016514
Physical Location & Seq
Forward (+)
21598506 .. 21600183
1678 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20020.4

Sequence Viewer

Length: 408 bp
ATGGCGGCAGCTCAAATGCAGAGAGTCGCATCTCCACCGCCGCAATCGGTTGAGGCGCCGAAGAAGAACAGAATTCAGGTCTCCAACACCAAGAAACCGCTCTTCTTCTACGTCAATCTCGCTAAGAGGTACATAGAGCAGTACAACGAGGTTGAGCTCTCTTCGCTTGGGATGGCAATCACCACGGTTATCACTATTGCCGAGATTTTGAAGAACAACGGCTTGGCTATCGAGAAGAAGGTGTCTACATCTACCGTTGGAATGAAAGACGAGAGTAAGGGTCGCCTCGTGCAGAAGGCTAAGATCGAGATTGTGCTGGGGAAGTCTGAAAAATTCGATGCCATAATGCAAATGAATGCTGATGTACTTGCTGCAGACTCGGTTGCGACCGAGGGAACAAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

9.55

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04620
fragaria_vesca FvH4_6g02590
malus_domestica MD04G1226900.v1.1 MD12G1243900.v1.1
prunus_persica Prupe.6G345600_v2.0.a1
pyrus_communis pycom04g20020
rosa_chinensis RchiOBHm_Chr3g0450501
rosa_laevigata RLG00000025726
rosa_multiflora Rmu_sc0000078.1_g000053
rosa_roxburghii Rroxscaffold_6G00428180
rosa_rugosa Rorug02G0628100
rosa_samantha Rh3BG029300 Rh3DG029000
rosa_wichuraiana Rw3G002220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 245
AciI CCGC 4 cut(s) 5, 38, 41, 98
AcsI RAATTY 2 cut(s) 72, 332
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 3 cut(s) 131, 143, 366
AgsI TTSAA 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 2 cut(s) 11, 157
AluI AGCT 2 cut(s) 11, 157
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 8, 371
ApoI RAATTY 2 cut(s) 72, 332
AspLEI GCGC 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 172
BanI GGYRCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 20, 358
BccI CCATC 1 cut(s) 166
BceAI ACGGC 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 85
BfmI CTRYAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 59
BisI GCNGC 4 cut(s) 6, 9, 41, 372
BlsI GCNGC 4 cut(s) 7, 10, 42, 373
BmiI GGNNCC 1 cut(s) 57
BmsI GCATC 2 cut(s) 38, 328
BsaBI GATNNNNATC 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 183, 390
Bse8I GATNNNNATC 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 183, 390
BseGI GGATG 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 176
BseXI GCAGC 2 cut(s) 20, 358
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 390
BshNI GGYRCC 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 361
Bso31I GGTCTC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 303
BspACI CCGC 4 cut(s) 5, 38, 41, 98
BspLI GGNNCC 1 cut(s) 57
BspMAI CTGCAG 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 183, 390
BssMI GATC 1 cut(s) 303
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 187, 256
Bst6I CTCTTC 2 cut(s) 107, 166
BstACI GRCGYC 1 cut(s) 56
BstDEI CTNAG 2 cut(s) 123, 300
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 1 cut(s) 303
BstMCI CGRYCG 1 cut(s) 390
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 20, 358
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 177
CfoI GCGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 130, 142, 365
CviJI RGCY 5 cut(s) 11, 157, 222, 227, 299
CviKI_1 RGCY 5 cut(s) 11, 157, 222, 227, 299
CviQI GTAC 3 cut(s) 130, 142, 365
DdeI CTNAG 2 cut(s) 123, 300
DinI GGCGCC 1 cut(s) 57
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 107, 166
EarI CTCTTC 2 cut(s) 107, 166
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
FaiI YATR 2 cut(s) 134, 344
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 4 cut(s) 6, 9, 41, 372
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 6, 9, 41, 372
GlaI GCGC 1 cut(s) 57
GluI GCNGC 4 cut(s) 6, 9, 41, 372
GsaI CCCAGC 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 59
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 2 cut(s) 24, 377
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 2 cut(s) 232, 307
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 2 cut(s) 232, 289
HpyCH4III ACNGT 2 cut(s) 187, 256
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 4 cut(s) 19, 292, 349, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 2 cut(s) 123, 300
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 56
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 303
LguI GCTCTTC 1 cut(s) 107
LpnPI CCDG 2 cut(s) 62, 302
Lsp1109I GCAGC 2 cut(s) 20, 358
LweI GCATC 2 cut(s) 38, 328
MaeII ACGT 1 cut(s) 111
MalI GATC 1 cut(s) 305
MbiI CCGCTC 1 cut(s) 100
MboI GATC 1 cut(s) 303
MboII GAAGA 7 cut(s) 73, 76, 94, 97, 153, 223, 247
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 2 cut(s) 72, 332
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 2 cut(s) 33, 371
MmeI TCCRAC 2 cut(s) 108, 238
MnlI CCTC 5 cut(s) 46, 120, 142, 296, 385
Mva1269I GAATGC 1 cut(s) 361
MwoI GCNNNNNNNGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 56
NdeII GATC 1 cut(s) 303
NlaIV GGNNCC 1 cut(s) 57
NmeAIII GCCGAG 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 107
PctI GAATGC 1 cut(s) 361
PkrI GCNGC 4 cut(s) 7, 10, 42, 373
PleI GAGTC 2 cut(s) 32, 371
PluTI GGCGCC 1 cut(s) 59
PpsI GAGTC 2 cut(s) 32, 371
Psp124BI GAGCTC 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 57
PstI CTGCAG 1 cut(s) 376
RsaI GTAC 3 cut(s) 131, 143, 366
RsaNI GTAC 3 cut(s) 130, 142, 365
SacI GAGCTC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 107
SatI GCNGC 4 cut(s) 6, 9, 41, 372
Sau3AI GATC 1 cut(s) 303
SchI GAGTC 2 cut(s) 33, 371
SduI GDGCHC 1 cut(s) 159
SetI ASST 7 cut(s) 13, 81, 114, 131, 153, 159, 243
SfaNI GCATC 2 cut(s) 38, 328
SfcI CTRYAG 1 cut(s) 372
SfoI GGCGCC 1 cut(s) 57
Sse9I AATT 2 cut(s) 72, 332
SsiI CCGC 4 cut(s) 5, 38, 41, 98
SspDI GGCGCC 1 cut(s) 55
SstI GAGCTC 1 cut(s) 159
TaaI ACNGT 2 cut(s) 187, 256
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 231, 306, 336
TaqII GACCGA 1 cut(s) 404
TasI AATT 2 cut(s) 72, 332
TatI WGTACW 2 cut(s) 141, 364
TauI GCSGC 2 cut(s) 8, 43
TseI GCWGC 2 cut(s) 8, 371
TspDTI ATGAA 2 cut(s) 278, 368
XapI RAATTY 2 cut(s) 72, 332
XmiI GTMKAC 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.