RLG00000025726

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016514
Physical Location & Seq
Forward (+)
47910753 .. 47912188
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025726

Sequence Viewer

Length: 510 bp
ATGGCGTCGGTTCAGGTAGCGAGGGTTGCAGCCGTACCACCACCGCAGGCCCAGGCTCAGGCTCAGGCTCAGCCCCAGGTCCAGGTCCAGGTCCAGACCCAGACTCAGACCCAGACCCAGACCCAGAGCCAGACCCAGGCCGCACAGGCCACAGGAGAGCAGCAGAAGAAGAACAGAATTCAAGTTTCCAATACCAAGAAGCCGCTCTTCTTCTACGTCAATCTTGCTAAGAGGTATATAGAGCAGCACAATGAGGTTGAGCTCTCTGCATTGGGAATGGCAATCACTACTGTTGTCACTATTGCTGAGATTTTGAAGAACAATGGACTGGCTATTGAAAAGAAGGTGTCTACATCTACGGTTGGCATGAGGGATGACAACAAGGGTCGTCTGGTGCAGAAGGCTAAGATTGAGATTGTGCTGGGGAAGTCTGAAAAGTTTGACTCTATAATGAGTGCTGCCGCAGCTGCTCAAGCTGCTCAGGAGTCAGCTGCTGAGGGCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

170

Amino Acids

18.22

Weight (kDa)

9.73

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 58 - 119 2.6e-13 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04620
fragaria_vesca FvH4_6g02590
malus_domestica MD04G1226900.v1.1 MD12G1243900.v1.1
prunus_persica Prupe.6G345600_v2.0.a1
pyrus_communis pycom04g20020
rosa_chinensis RchiOBHm_Chr3g0450501
rosa_laevigata RLG00000025726
rosa_multiflora Rmu_sc0000078.1_g000053
rosa_roxburghii Rroxscaffold_6G00428180
rosa_rugosa Rorug02G0628100
rosa_samantha Rh3BG029300 Rh3DG029000
rosa_wichuraiana Rw3G002220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 350
AciI CCGC 4 cut(s) 44, 141, 203, 462
AcsI RAATTY 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 36
AfiI CCNNNNNNNGG 4 cut(s) 58, 82, 88, 136
AgsI TTSAA 3 cut(s) 182, 316, 338
AjnI CCWGG 5 cut(s) 51, 75, 81, 87, 135
AluBI AGCT 4 cut(s) 262, 467, 476, 491
AluI AGCT 4 cut(s) 262, 467, 476, 491
Alw21I GWGCWC 1 cut(s) 264
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 3 cut(s) 48, 138, 147
ApeKI GCWGC 8 cut(s) 29, 160, 244, 458, 464, 467, 476, 491
ApoI RAATTY 1 cut(s) 177
AspS9I GGNCC 4 cut(s) 49, 79, 85, 91
AvaII GGWCC 3 cut(s) 79, 85, 91
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 8 cut(s) 41, 172, 256, 445, 454, 463, 476, 478
BceAI ACGGC 1 cut(s) 17
BciT130I CCWGG 5 cut(s) 53, 77, 83, 89, 137
BglI GCCNNNNNGGC 1 cut(s) 146
BlpI GCTNAGC 1 cut(s) 69
Bme1390I CCNGG 5 cut(s) 53, 77, 83, 89, 137
Bme18I GGWCC 3 cut(s) 79, 85, 91
BmgT120I GGNCC 4 cut(s) 49, 79, 85, 91
BmrFI CCNGG 5 cut(s) 53, 77, 83, 89, 137
Bpu10I CCTNAGC 4 cut(s) 57, 63, 480, 495
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 51, 75, 135
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 82, 88, 136
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 5 cut(s) 53, 77, 83, 89, 137
BseDI CCNNGG 3 cut(s) 51, 75, 135
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 4 cut(s) 58, 82, 88, 136
BseMII CTCAG 7 cut(s) 71, 77, 83, 119, 297, 486, 494
BseNI ACTGG 1 cut(s) 333
BseXI GCAGC 8 cut(s) 41, 172, 256, 445, 454, 463, 476, 478
BseYI CCCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 416
BshFI GGCC 3 cut(s) 50, 140, 149
BsiHKAI GWGCWC 1 cut(s) 264
BslI CCNNNNNNNGG 4 cut(s) 58, 82, 88, 136
BsnI GGCC 3 cut(s) 50, 140, 149
Bsp1286I GDGCHC 1 cut(s) 264
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 4 cut(s) 44, 141, 203, 462
BspANI GGCC 3 cut(s) 50, 140, 149
BspCNI CTCAG 7 cut(s) 70, 76, 82, 118, 298, 487, 493
BspQI GCTCTTC 1 cut(s) 212
BsrBI CCGCTC 1 cut(s) 205
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 3 cut(s) 51, 75, 135
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 5 cut(s) 53, 77, 83, 89, 137
Bst4CI ACNGT 2 cut(s) 292, 361
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 9 cut(s) 57, 63, 69, 105, 228, 306, 405, 480, 495
BstF5I GGATG 1 cut(s) 379
BstMWI GCNNNNNNNGC 6 cut(s) 26, 146, 464, 467, 473, 476
BstNI CCWGG 5 cut(s) 53, 77, 83, 89, 137
BstSCI CCNGG 5 cut(s) 51, 75, 81, 87, 135
BstV1I GCAGC 8 cut(s) 41, 172, 256, 445, 454, 463, 476, 478
BsuRI GGCC 3 cut(s) 50, 140, 149
BtsCI GGATG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 48
CaiI CAGNNNCTG 1 cut(s) 494
Cfr13I GGNCC 4 cut(s) 49, 79, 85, 91
Csp6I GTAC 1 cut(s) 35
CviAII CATG 1 cut(s) 367
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 9 cut(s) 57, 63, 69, 105, 228, 306, 405, 480, 495
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 3 cut(s) 79, 85, 91
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 177
EcoRII CCWGG 5 cut(s) 51, 75, 81, 87, 135
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 1 cut(s) 370
FaiI YATR 4 cut(s) 237, 239, 368, 449
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 350
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 1 cut(s) 264
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 3 cut(s) 50, 140, 149
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 370
HinfI GANTC 3 cut(s) 103, 443, 485
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 2 cut(s) 108, 433
Hpy188III TCNNGA 2 cut(s) 94, 482
Hpy8I GTNNAC 1 cut(s) 351
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 337, 394
HpyCH4III ACNGT 2 cut(s) 292, 361
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 3 cut(s) 29, 269, 397
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 146, 464, 467, 473, 476
HpyF3I CTNAG 9 cut(s) 57, 63, 69, 105, 228, 306, 405, 480, 495
HpySE526I ACGT 1 cut(s) 216
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 212
Lsp1109I GCAGC 8 cut(s) 41, 172, 256, 445, 454, 463, 476, 478
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 295
MbiI CCGCTC 1 cut(s) 205
MboII GAAGA 5 cut(s) 178, 181, 199, 202, 328
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 1 cut(s) 177
MlyI GAGTC 3 cut(s) 97, 437, 494
MnlI CCTC 5 cut(s) 15, 225, 247, 363, 490
MspA1I CMGCKG 2 cut(s) 467, 491
MspR9I CCNGG 5 cut(s) 53, 77, 83, 89, 137
MvaI CCWGG 5 cut(s) 53, 77, 83, 89, 137
MwoI GCNNNNNNNGC 6 cut(s) 26, 146, 464, 467, 473, 476
NlaIII CATG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 212
PleI GAGTC 3 cut(s) 97, 437, 493
PpsI GAGTC 3 cut(s) 97, 437, 493
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 5 cut(s) 51, 75, 81, 87, 135
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 5 cut(s) 51, 75, 81, 87, 135
PspPI GGNCC 4 cut(s) 49, 79, 85, 91
PstNI CAGNNNCTG 1 cut(s) 494
PvuII CAGCTG 2 cut(s) 467, 491
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 212
Sau96I GGNCC 4 cut(s) 49, 79, 85, 91
SchI GAGTC 3 cut(s) 97, 437, 494
ScrFI CCNGG 5 cut(s) 53, 77, 83, 89, 137
SduI GDGCHC 1 cut(s) 264
SfiI GGCCNNNNNGGCC 1 cut(s) 146
SinI GGWCC 3 cut(s) 79, 85, 91
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 1 cut(s) 177
SsiI CCGC 4 cut(s) 44, 141, 203, 462
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 5 cut(s) 51, 75, 81, 87, 135
TaaI ACNGT 2 cut(s) 292, 361
TaiI ACGT 1 cut(s) 219
TasI AATT 1 cut(s) 177
TauI GCSGC 3 cut(s) 143, 205, 464
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 8 cut(s) 29, 160, 244, 458, 464, 467, 476, 491
Tsp45I GTSAC 1 cut(s) 295
VpaK11BI GGWCC 3 cut(s) 79, 85, 91
XapI RAATTY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.