pycom05g00090

Single-stranded DNA-bindig protein WHY2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011300
Physical Location & Seq
Forward (+)
141130 .. 143818
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 945 bp
ATGGTATTAATTTCTTCTCATGTGGGCGAGTCAAGGCTCGGTCCATCTTGTCACGGGCCGGCCCGAACAAGCTCAAAAAGCCCTGGCTCGTGTGGACCGGTTGAGTCGAGAAAAACGGCAAGTCGTCTCGATATTCCAAAGGTATCAAAGCAGAAGTGCAGAAGCATGAGCAGTGGCATTCCCCACCCCCCCCCCCCCCCCCCCCTCTCTCTCTCTCTCTCTCGAGCAACAAAGATGTTGAAGGTGTTGCGCGTCTTGTCCTCTTCCACCGCGAAATTCAGGAGCCATATCTGCACTAGAGATGCTTTATCAATGTATGCTTTTACTCATATCACCAGATTTTCAACTGCCACTCAGAAATTTTCTGTTAAAGGTCCCAGTTCTCATCAAGTATATGCTTCTTTTGATATCTTCAAAGGCAAAGCTGCCCTCTCTTTGACTCCTGTTCTTCCAACATTCACTAAATTGGAATCTGGAAGTCTTGTAGTTGATCGGCGTGGTTCTGTCATGTTGAAGTTCACTCCTGCCATTGGTGAACGTAAGTATGACTGGGAAAAGAGACAGATGTTTGCTTTATCAGCTACAGAGGTCGGCGCTCTGATAAGCTTGGGTCCCAATGATTCTTGTGAACTATTTCATGATCCCTCAATGAAATCAAGTAATGCTGGTCAAGTGAGGAAGAGCTTATCGATTAAGCCTCATGCGGATGGCAGTGGCTACTTTGTTTCCTTAACTGTTGTCAACAACCTGCTAAAGACCAGAGAGAGTTTCTCAGTTCCTGTCACGACTGCTGAATTTGCTGTTATGAAGACTGCTTGCAGTTTTGCATTGCCCCACATCATGGGTTGGGATAGATTGACGAACAAGATGCCAGCAGGTGGTGGTGGTGGAGGAGGGCAAGAATCAAAGGCGGTTCCACAGCTTTTGGAGGAGTGGGATAGATGA

Protein Analysis

315

Amino Acids

33.87

Weight (kDa)

9.93

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Whirly PF08536 137 - 271 2.6e-60 Whirly transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 866
Acc36I ACCTGC 2 cut(s) 756, 866
AccB7I CCANNNNNTGG 2 cut(s) 841, 878
AccII CGCG 2 cut(s) 252, 272
AciI CCGC 3 cut(s) 270, 704, 911
AclWI GGATC 1 cut(s) 635
AcsI RAATTY 3 cut(s) 275, 359, 794
AfiI CCNNNNNNNGG 3 cut(s) 530, 841, 878
AgeI ACCGGT 1 cut(s) 97
AgsI TTSAA 4 cut(s) 241, 345, 415, 514
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 6 cut(s) 72, 425, 581, 606, 684, 922
AluI AGCT 6 cut(s) 72, 425, 581, 606, 684, 922
Alw26I GTCTC 2 cut(s) 131, 553
AlwI GGATC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 222
AoxI GGCC 2 cut(s) 56, 60
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 3 cut(s) 275, 359, 794
AseI ATTAAT 1 cut(s) 8
AsiGI ACCGGT 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 2 cut(s) 252, 596
AspS9I GGNCC 6 cut(s) 41, 56, 61, 95, 374, 611
AsuHPI GGTGA 2 cut(s) 325, 545
AvaI CYCGRG 1 cut(s) 222
AvaII GGWCC 4 cut(s) 41, 95, 374, 611
BauI CACGAG 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 815
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 52, 701
BceAI ACGGC 1 cut(s) 132
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 1 cut(s) 84
BcoDI GTCTC 2 cut(s) 131, 553
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 2 cut(s) 756, 866
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 4 cut(s) 41, 95, 374, 611
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 6 cut(s) 41, 56, 61, 95, 374, 611
BmiI GGNNCC 5 cut(s) 284, 376, 612, 613, 915
BmrFI CCNGG 1 cut(s) 84
BmrI ACTGGG 2 cut(s) 372, 559
BmsI GCATC 2 cut(s) 292, 858
BmuI ACTGGG 2 cut(s) 372, 559
BpiI GAAGAC 1 cut(s) 815
BplI GAGNNNNNCTC 2 cut(s) 755, 787
Bsa29I ATCGAT 1 cut(s) 689
BsaJI CCNNGG 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 841, 878
Bse118I RCCGGY 2 cut(s) 58, 97
Bse1I ACTGG 2 cut(s) 378, 554
Bse3DI GCAATG 1 cut(s) 827
BseBI CCWGG 1 cut(s) 84
BseCI ATCGAT 1 cut(s) 689
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 712
BseLI CCNNNNNNNGG 3 cut(s) 530, 841, 878
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 2 cut(s) 368, 786
BseNI ACTGG 2 cut(s) 378, 554
BseRI GAGGAG 2 cut(s) 906, 944
BseXI GCAGC 1 cut(s) 412
BsgI GTGCAG 2 cut(s) 178, 277
Bsh1236I CGCG 2 cut(s) 252, 272
BshFI GGCC 2 cut(s) 58, 62
BshTI ACCGGT 1 cut(s) 97
BshVI ATCGAT 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 2 cut(s) 59, 98
BslFI GGGAC 2 cut(s) 360, 597
BslI CCNNNNNNNGG 3 cut(s) 530, 841, 878
BsmAI GTCTC 2 cut(s) 131, 553
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 360, 597
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 2 cut(s) 58, 62
BsoBI CYCGRG 1 cut(s) 222
Bsp143I GATC 2 cut(s) 490, 640
BspACI CCGC 3 cut(s) 270, 704, 911
BspANI GGCC 2 cut(s) 58, 62
BspCNI CTCAG 2 cut(s) 367, 785
BspDI ATCGAT 1 cut(s) 689
BspFNI CGCG 2 cut(s) 252, 272
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 5 cut(s) 284, 376, 612, 613, 915
BspMI ACCTGC 2 cut(s) 756, 866
BspPI GGATC 1 cut(s) 635
BspQI GCTCTTC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 827
BsrFI RCCGGY 2 cut(s) 58, 97
BsrI ACTGG 2 cut(s) 378, 554
BssAI RCCGGY 2 cut(s) 58, 97
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 2 cut(s) 490, 640
BssSI CACGAG 1 cut(s) 88
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 736
Bst6I CTCTTC 2 cut(s) 268, 674
BstC8I GCNNGC 3 cut(s) 60, 817, 873
BstDEI CTNAG 2 cut(s) 354, 772
BstF5I GGATG 1 cut(s) 712
BstFNI CGCG 2 cut(s) 252, 272
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 2 cut(s) 252, 596
BstKTI GATC 2 cut(s) 493, 643
BstMAI GTCTC 2 cut(s) 131, 553
BstMBI GATC 2 cut(s) 490, 640
BstMWI GCNNNNNNNGC 4 cut(s) 78, 291, 578, 797
BstNI CCWGG 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 2 cut(s) 252, 272
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 1 cut(s) 815
Bsu15I ATCGAT 1 cut(s) 689
BsuRI GGCC 2 cut(s) 58, 62
BsuTUI ATCGAT 1 cut(s) 689
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 2 cut(s) 178, 718
BtsIMutI CAGTG 2 cut(s) 178, 718
BveI ACCTGC 2 cut(s) 756, 866
Cac8I GCNNGC 3 cut(s) 60, 817, 873
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 637
CfoI GCGC 2 cut(s) 252, 596
Cfr10I RCCGGY 2 cut(s) 58, 97
Cfr13I GGNCC 6 cut(s) 41, 56, 61, 95, 374, 611
ClaI ATCGAT 1 cut(s) 689
CseI GACGC 1 cut(s) 241
CspAI ACCGGT 1 cut(s) 97
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
CviAII CATG 6 cut(s) 20, 166, 508, 638, 701, 841
DdeI CTNAG 2 cut(s) 354, 772
DpnI GATC 2 cut(s) 492, 642
DpnII GATC 2 cut(s) 490, 640
Eam1104I CTCTTC 2 cut(s) 268, 674
EarI CTCTTC 2 cut(s) 268, 674
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 41, 95, 374, 611
Eco88I CYCGRG 1 cut(s) 222
EcoO109I RGGNCCY 2 cut(s) 374, 611
EcoRII CCWGG 1 cut(s) 82
EcoRV GATATC 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 6 cut(s) 23, 169, 511, 641, 704, 844
FaqI GGGAC 2 cut(s) 360, 597
FatI CATG 6 cut(s) 19, 165, 507, 637, 700, 840
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 1 cut(s) 719
FseI GGCCGGCC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 1 cut(s) 297
GlaI GCGC 2 cut(s) 251, 595
GluI GCNGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 2 cut(s) 58, 62
HapII CCGG 2 cut(s) 59, 98
HgaI GACGC 1 cut(s) 241
HhaI GCGC 2 cut(s) 252, 596
Hin1II CATG 6 cut(s) 23, 169, 511, 641, 704, 844
Hin6I GCGC 2 cut(s) 250, 594
HinP1I GCGC 2 cut(s) 250, 594
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 6 cut(s) 29, 104, 439, 470, 620, 902
HpaII CCGG 2 cut(s) 59, 98
HphI GGTGA 2 cut(s) 325, 545
Hpy166II GTNNAC 5 cut(s) 95, 519, 536, 629, 742
Hpy188I TCNGA 2 cut(s) 357, 600
Hpy188III TCNNGA 7 cut(s) 108, 128, 222, 280, 474, 638, 784
Hpy8I GTNNAC 5 cut(s) 95, 519, 536, 629, 742
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 736
HpyCH4IV ACGT 1 cut(s) 538
HpyCH4V TGCA 4 cut(s) 159, 294, 819, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 291, 578, 797
HpyF3I CTNAG 2 cut(s) 354, 772
HpySE526I ACGT 1 cut(s) 538
Hsp92II CATG 6 cut(s) 23, 169, 511, 641, 704, 844
HspAI GCGC 2 cut(s) 250, 594
KflI GGGWCCC 1 cut(s) 611
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 490, 640
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 1 cut(s) 282
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 292, 858
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 538
MaeIII GTNAC 2 cut(s) 50, 781
MalI GATC 2 cut(s) 492, 642
MboI GATC 2 cut(s) 490, 640
MboII GAAGA 6 cut(s) 6, 255, 403, 440, 691, 820
MluCI AATT 5 cut(s) 9, 275, 359, 464, 794
MlyI GAGTC 3 cut(s) 38, 113, 433
MmeI TCCRAC 1 cut(s) 476
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 4 cut(s) 8, 369, 693, 731
MslI CAYNNNNRTG 1 cut(s) 705
MspI CCGG 2 cut(s) 59, 98
MspR9I CCNGG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 1 cut(s) 84
MvnI CGCG 2 cut(s) 252, 272
MwoI GCNNNNNNNGC 4 cut(s) 78, 291, 578, 797
NaeI GCCGGC 1 cut(s) 60
NdeII GATC 2 cut(s) 490, 640
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 6 cut(s) 23, 169, 511, 641, 704, 844
NlaIV GGNNCC 5 cut(s) 284, 376, 612, 613, 915
NmuCI GTSAC 2 cut(s) 50, 781
PaeR7I CTCGAG 1 cut(s) 222
PagI TCATGA 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 866
PciSI GCTCTTC 1 cut(s) 674
PctI GAATGC 1 cut(s) 177
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 470, 620, 902
PflMI CCANNNNNTGG 2 cut(s) 841, 878
PinAI ACCGGT 1 cut(s) 97
PkrI GCNGC 1 cut(s) 427
PleI GAGTC 3 cut(s) 37, 112, 433
PpsI GAGTC 3 cut(s) 37, 112, 433
PpuMI RGGWCCY 2 cut(s) 374, 611
PshBI ATTAAT 1 cut(s) 8
Psp5II RGGWCCY 2 cut(s) 374, 611
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 5 cut(s) 284, 376, 612, 613, 915
PspPI GGNCC 6 cut(s) 41, 56, 61, 95, 374, 611
PspPPI RGGWCCY 2 cut(s) 374, 611
PstNI CAGNNNCTG 1 cut(s) 779
RigI GGCCGGCC 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 705
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 4 cut(s) 8, 369, 693, 731
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 2 cut(s) 490, 640
Sau96I GGNCC 6 cut(s) 41, 56, 61, 95, 374, 611
SchI GAGTC 3 cut(s) 38, 113, 433
ScrFI CCNGG 1 cut(s) 84
SfaNI GCATC 2 cut(s) 292, 858
SfcI CTRYAG 1 cut(s) 582
Sfr274I CTCGAG 1 cut(s) 222
SinI GGWCC 4 cut(s) 41, 95, 374, 611
SlaI CTCGAG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 5 cut(s) 9, 275, 359, 464, 794
SsiI CCGC 3 cut(s) 270, 704, 911
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 736
TaiI ACGT 1 cut(s) 541
TaqI TCGA 4 cut(s) 107, 129, 223, 689
TaqII GACCGA 1 cut(s) 29
TasI AATT 5 cut(s) 9, 275, 359, 464, 794
TfiI GAWTC 3 cut(s) 470, 620, 902
Tru1I TTAA 4 cut(s) 8, 369, 693, 731
Tru9I TTAA 4 cut(s) 8, 369, 693, 731
TscAI CASTG 2 cut(s) 178, 718
TseFI GTSAC 2 cut(s) 50, 781
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 2 cut(s) 50, 781
TspDTI ATGAA 3 cut(s) 626, 665, 821
TspRI CASTG 2 cut(s) 178, 718
Van91I CCANNNNNTGG 2 cut(s) 841, 878
VpaK11BI GGWCC 4 cut(s) 41, 95, 374, 611
VspI ATTAAT 1 cut(s) 8
XapI RAATTY 3 cut(s) 275, 359, 794
XhoI CTCGAG 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.