RchiOBHm_Chr6g0249551

Single-stranded DNA-bindig protein WHY2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011300
Physical Location & Seq
Forward (+)
5528274 .. 5532421
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22373

Sequence Viewer

Length: 690 bp
ATGATGCAGCTTTTGCGCGTCTTCTCCTCCTCCTCCAGGAACCAGTTGTCAAAGAGAGCTGCTTTATCGTTGGATTCTTTTACACAAAGCACCAGCTTTTCAACTGCCAGGCAGAATTTTTCTGCTAAAGGAAGAATCGGTGATCAAGTATATGCTCCTTTTTCTATCTACAAAGGCAAAGCTGCACTCTCCTTGAGTCCTGTTCTCCCAACATTCACTAAATTGGAATCTGGAAGTGTTGTGGTTGAGCGACGTGGTTCCGTGATGTTAAAGTTCATGCCTGCAATTGGTGAGCGCAAATATGACTCGGAGAAGAAACAGTTGTTTGCTTTATCGGCAACAGAGGTTGGGTCTCTAATCAGCTTGGGTCCCAAAGATTCATGTGAATTATTTCATGATCCCTCTATGCAATCAAGTAATGCTGGTCAAGTGAGGAAGAGCTTATCAATCAAGCCTCATGCAGATGGCTATTTTATTTCTTTGACTGTTGTCAACAACCTACTGAAAACCAGAGAGAGTTTCTCAGTTCCTGTCACAACGGCTGAATTTGCTGTAATGAAGACTGCTTGCAGTTTTGCACTTCCCCACATCATGGGTTGGGATCGGTTGACAAATAAGGTGCCAAGAGGCATAGAAGTGCACAAATCAGAGATGGCAGAACCACAACTTCTGGATGAATGGGAGAGATGA

Protein Analysis

229

Amino Acids

25.33

Weight (kDa)

9.57

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Whirly PF08536 56 - 188 7.6e-57 Whirly transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 619
AccB7I CCANNNNNTGG 1 cut(s) 592
AccII CGCG 1 cut(s) 18
AclWI GGATC 2 cut(s) 392, 609
AcsI RAATTY 2 cut(s) 115, 545
AfiI CCNNNNNNNGG 3 cut(s) 36, 287, 592
AgsI TTSAA 1 cut(s) 102
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 2 cut(s) 35, 107
AluBI AGCT 6 cut(s) 10, 59, 96, 182, 363, 441
AluI AGCT 6 cut(s) 10, 59, 96, 182, 363, 441
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 1 cut(s) 357
Alw44I GTGCAC 1 cut(s) 638
AlwI GGATC 2 cut(s) 392, 609
AlwNI CAGNNNCTG 1 cut(s) 530
ApaLI GTGCAC 1 cut(s) 638
ApeKI GCWGC 3 cut(s) 7, 59, 182
ApoI RAATTY 2 cut(s) 115, 545
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 2 cut(s) 18, 297
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 152, 302
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 642
BanI GGYRCC 1 cut(s) 619
BbsI GAAGAC 2 cut(s) 13, 566
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 3 cut(s) 19, 46, 169
BccI CCATC 2 cut(s) 458, 646
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 2 cut(s) 37, 109
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 357
BisI GCNGC 3 cut(s) 8, 60, 183
BlsI GCNGC 3 cut(s) 9, 61, 184
Bme1390I CCNGG 2 cut(s) 37, 109
Bme18I GGWCC 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 5 cut(s) 41, 259, 369, 370, 621
BmrFI CCNGG 2 cut(s) 37, 109
BoxI GACNNNNGTC 1 cut(s) 488
BpiI GAAGAC 2 cut(s) 13, 566
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BpmI CTGGAG 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 357
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 287, 592
Bse1I ACTGG 1 cut(s) 43
BseBI CCWGG 2 cut(s) 37, 109
BseGI GGATG 1 cut(s) 679
BseLI CCNNNNNNNGG 3 cut(s) 36, 287, 592
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 3 cut(s) 16, 19, 22
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 3 cut(s) 19, 46, 169
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 18
BshNI GGYRCC 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 642
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 36, 287, 592
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 354
Bso31I GGTCTC 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 3 cut(s) 142, 397, 601
BspCNI CTCAG 1 cut(s) 536
BspFNI CGCG 1 cut(s) 18
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 5 cut(s) 41, 259, 369, 370, 621
BspPI GGATC 2 cut(s) 392, 609
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 357
BsrI ACTGG 1 cut(s) 43
BssMI GATC 3 cut(s) 142, 397, 601
Bst2UI CCWGG 2 cut(s) 37, 109
Bst4CI ACNGT 2 cut(s) 321, 487
Bst6I CTCTTC 1 cut(s) 431
BstAPI GCANNNNNTGC 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 282, 568
BstDEI CTNAG 1 cut(s) 523
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 679
BstFNI CGCG 1 cut(s) 18
BstHHI GCGC 2 cut(s) 18, 297
BstKTI GATC 3 cut(s) 145, 400, 604
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 3 cut(s) 142, 397, 601
BstMWI GCNNNNNNNGC 3 cut(s) 13, 335, 548
BstNI CCWGG 2 cut(s) 37, 109
BstPAI GACNNNNGTC 1 cut(s) 488
BstSCI CCNGG 2 cut(s) 35, 107
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 3 cut(s) 19, 46, 169
BstV2I GAAGAC 2 cut(s) 13, 566
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 282, 568
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 394
CfoI GCGC 2 cut(s) 18, 297
Cfr13I GGNCC 1 cut(s) 368
CseI GACGC 1 cut(s) 7
CviAII CATG 5 cut(s) 277, 381, 395, 458, 592
CviJI RGCY 9 cut(s) 10, 59, 96, 182, 363, 441, 454, 468, 542
CviKI_1 RGCY 9 cut(s) 10, 59, 96, 182, 363, 441, 454, 468, 542
DdeI CTNAG 1 cut(s) 523
DpnI GATC 3 cut(s) 144, 399, 603
DpnII GATC 3 cut(s) 142, 397, 601
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 35, 107
FaeI CATG 5 cut(s) 280, 384, 398, 461, 595
FaqI GGGAC 1 cut(s) 354
FatI CATG 5 cut(s) 276, 380, 394, 457, 591
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 3 cut(s) 8, 60, 183
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 3 cut(s) 8, 60, 183
GlaI GCGC 2 cut(s) 17, 296
GluI GCNGC 3 cut(s) 8, 60, 183
GsuI CTGGAG 1 cut(s) 19
HgaI GACGC 1 cut(s) 7
HhaI GCGC 2 cut(s) 18, 297
Hin1II CATG 5 cut(s) 280, 384, 398, 461, 595
Hin6I GCGC 2 cut(s) 16, 295
HinP1I GCGC 2 cut(s) 16, 295
HincII GTYRAC 2 cut(s) 493, 609
HindII GTYRAC 2 cut(s) 493, 609
HinfI GANTC 6 cut(s) 74, 135, 196, 227, 305, 377
HphI GGTGA 2 cut(s) 152, 302
Hpy166II GTNNAC 3 cut(s) 493, 609, 640
Hpy188I TCNGA 2 cut(s) 310, 649
Hpy188III TCNNGA 3 cut(s) 231, 395, 671
Hpy8I GTNNAC 3 cut(s) 493, 609, 640
Hpy99I CGWCG 1 cut(s) 255
HpyCH4III ACNGT 2 cut(s) 321, 487
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 8 cut(s) 7, 185, 284, 409, 461, 570, 578, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 335, 548
HpyF3I CTNAG 1 cut(s) 523
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 5 cut(s) 280, 384, 398, 461, 595
HspAI GCGC 2 cut(s) 16, 295
KflI GGGWCCC 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 3 cut(s) 142, 397, 601
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 3 cut(s) 19, 46, 169
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 532
MalI GATC 3 cut(s) 144, 399, 603
MboI GATC 3 cut(s) 142, 397, 601
MboII GAAGA 5 cut(s) 13, 144, 325, 448, 571
MfeI CAATTG 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 5 cut(s) 115, 221, 285, 386, 545
MlyI GAGTC 2 cut(s) 205, 299
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 8 cut(s) 37, 40, 43, 337, 412, 426, 465, 620
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 2 cut(s) 462, 635
MspR9I CCNGG 2 cut(s) 37, 109
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 2 cut(s) 37, 109
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 13, 335, 548
NdeII GATC 3 cut(s) 142, 397, 601
NlaIII CATG 5 cut(s) 280, 384, 398, 461, 595
NlaIV GGNNCC 5 cut(s) 41, 259, 369, 370, 621
NmuCI GTSAC 1 cut(s) 532
PagI TCATGA 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 4 cut(s) 74, 135, 227, 377
PflMI CCANNNNNTGG 1 cut(s) 592
PfoI TCCNGGA 1 cut(s) 35
PkrI GCNGC 3 cut(s) 9, 61, 184
PleI GAGTC 2 cut(s) 204, 299
PpsI GAGTC 2 cut(s) 204, 299
PpuMI RGGWCCY 1 cut(s) 368
PshAI GACNNNNGTC 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 35, 107
PspGI CCWGG 2 cut(s) 35, 107
PspN4I GGNNCC 5 cut(s) 41, 259, 369, 370, 621
PspPI GGNCC 1 cut(s) 368
PspPPI RGGWCCY 1 cut(s) 368
PstNI CAGNNNCTG 1 cut(s) 530
RseI CAYNNNNRTG 2 cut(s) 462, 635
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 3 cut(s) 8, 60, 183
Sau3AI GATC 3 cut(s) 142, 397, 601
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 2 cut(s) 205, 299
ScrFI CCNGG 2 cut(s) 37, 109
SduI GDGCHC 1 cut(s) 642
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 2 cut(s) 462, 635
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 5 cut(s) 115, 221, 285, 386, 545
StyD4I CCNGG 2 cut(s) 35, 107
TaaI ACNGT 2 cut(s) 321, 487
TaiI ACGT 1 cut(s) 256
TasI AATT 5 cut(s) 115, 221, 285, 386, 545
TfiI GAWTC 4 cut(s) 74, 135, 227, 377
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 3 cut(s) 7, 59, 182
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 5 cut(s) 265, 369, 383, 572, 690
TspGWI ACGGA 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 592
VneI GTGCAC 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 368
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 2 cut(s) 115, 545
XmnI GAANNNNTTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.