pycom05g00650

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007910
Physical Location & Seq
Forward (+)
836004 .. 837737
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00650.1

Sequence Viewer

Length: 1641 bp
ATGTCTTTAGTTCGGCCAGCAGAGCTCTCCACATCGTACAGAAATCCCAATTTCTACTCTCTAAGGGGCAGCAATGAAAGTTCCAGCTTGTCAACTCAAATATTTGGCTCTGATAAGCCCAAGGTGGTGTATTCAGATGATCCCTACAGCGCTGAGAGTTATGAGAAGTACTTCCTTGACTCCCCAATGGAAGAAGTTACACATCCATCCAGCTCCGGCGTTTCTGGGAGTTCAACTAACCCACAAGATTACTTTGAGAGTCAAAGCCCCGATGCGGATAGCTTTGATGAAGATAAGATGAGATTGAAGCTTCAAGAATTGGAGAGAGCACTCCTTAATGACAATGCAGATGAAGACGATGGGGAGAGAAATTGCAGTAGTCAAAGCATGGAAGTGGATGGTGAATGGATTGATCCAATCCAGAATGAATTGCTCCATGACTCACCCAAGGAGTCTTTGTCCTCTGACTCTAACGCGAGCAGTACCAGCAGCAACAAAGAAATATCACAGGTTTCTCCACGGACACCCAAGCAGCTGCTTTTTGAGTGCGCTGGTGCACTCTCGGAGGGAAATGTCGAAGAAGCATCAACAATGATAAATGAGCTCCGACAGATGGTATCAATTCAAGGAGATCCTACACAGAGGATTGCGGCTTACATGGTGGAAGGCCTAGCAGCTCGTGTGGCTTCCTCAGGAAAATTTCTTTACAAGTCTTTGAAATGCAAGGAACCCCCGTCCTCTTATCGCCTTGCAGCCATGCAAATCCTTTTTGAGGTGTGTCCGTGCTTTAAATTTGGATTTATGGCAGCAAATGGAGCCATCATAGAGGCATGCAAAGATGAAAAGAGAGTTCACATCATAGATTTTGACATAAACCAGGGGAACCAATACATAACCCTCATACAAACACTTTCGAGTCTGCCAGGCAAGCCACCACACTTGAAGTTAACAGGGGTGGATGATCCCGAGACAGTTCAGCGTCATGTTGGAGGCCTATCGATCATTGGACAAAGGCTTGAGAAGCTAGCAGAAGTGCTGAAAGTTCCATTCGAGTTTCATGCAGTAGCCTCAAGGACTTCAATCGTCAATACCTCAATGCTTGGCTGCAGGCCTGGGGAAGCACTTGTGGTTAACTTTGCTTTTCAGCTTCACCACATGCCAGACGAAAGTGTTTCAACGGTTAACCAGAGGGACCAGCTTCTTCGGATGGTGAAGAGCTTGAGGCCAAAACTTGTTACAGTTGTAGAACAAGACATGAACACCAATACGACCCCTTTTCTCCCAAGATTTGTTGAAGCCTACAACTATTACTCTGCTGTTTTTGATTCTCTTGATGCAGCTCTCCCCCGGGAGAGTCAGGATAGGATGAATGTCGAAAGGCAGTGTCTGGCACGGGACATAGTGAACATTGTAGCGTGCGAAGGAGAGGAAAGAATAGAGCGGTATGAGGTAGCTGGAAAGTGGAGGGCAAGGATGACCATGGCAGGATTTACTTCATGTCCTATGAGCACCAGTGTGACTGATTCGATTCGGGATCTTAGCAGACAGTACAGTGAGAGGTACAAGGTGAAGGAGGAGGCGGGGGCTCTTCTTTTCGGGTGGGAAGAGAAAAACTTAATTGTTGCTTCAGCGTGGAGGTGA

Protein Analysis

547

Amino Acids

61.03

Weight (kDa)

4.96

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 177 - 546 1e-140 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1441
AccII CGCG 1 cut(s) 476
AciI CCGC 4 cut(s) 275, 650, 1441, 1580
AclWI GGATC 5 cut(s) 134, 407, 626, 956, 1542
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 2 cut(s) 698, 791
AcuI CTGAAG 1 cut(s) 1611
AfaI GTAC 5 cut(s) 38, 170, 484, 1550, 1562
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 3 cut(s) 274, 613, 772
AgsI TTSAA 9 cut(s) 234, 307, 314, 626, 718, 943, 1080, 1176, 1295
AjnI CCWGG 3 cut(s) 876, 922, 1111
AleI CACNNNNGTG 1 cut(s) 1514
Alw21I GWGCWC 5 cut(s) 27, 331, 559, 606, 1511
Alw26I GTCTC 1 cut(s) 962
Alw44I GTGCAC 1 cut(s) 555
AlwI GGATC 5 cut(s) 134, 407, 626, 956, 1542
AlwNI CAGNNNCTG 1 cut(s) 1387
Ama87I CYCGRG 2 cut(s) 965, 1347
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 5 cut(s) 14, 667, 991, 1109, 1223
ApaLI GTGCAC 1 cut(s) 555
ApeKI GCWGC 9 cut(s) 69, 489, 532, 535, 674, 752, 806, 1104, 1337
ApoI RAATTY 2 cut(s) 698, 791
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 152, 551
AspS9I GGNCC 1 cut(s) 1192
AsuC2I CCSGG 2 cut(s) 1348, 1349
AsuHPI GGTGA 5 cut(s) 413, 435, 1142, 1222, 1579
AsuNHI GCTAGC 1 cut(s) 1024
AvaI CYCGRG 2 cut(s) 965, 1347
AvaII GGWCC 1 cut(s) 1192
AxyI CCTNAGG 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 559
BanII GRGCYC 3 cut(s) 27, 606, 1588
BauI CACGAG 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 5 cut(s) 27, 331, 559, 606, 1511
BbvI GCAGC 9 cut(s) 81, 501, 522, 544, 686, 764, 818, 1091, 1349
BccI CCATC 6 cut(s) 214, 353, 392, 607, 827, 1201
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 3 cut(s) 878, 924, 1113
BcnI CCSGG 2 cut(s) 1348, 1349
BcoDI GTCTC 1 cut(s) 962
BfaI CTAG 2 cut(s) 671, 1025
BfmI CTRYAG 2 cut(s) 145, 1105
BfoI RGCGCY 1 cut(s) 153
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 5 cut(s) 878, 924, 1113, 1348, 1349
Bme18I GGWCC 1 cut(s) 1192
BmeT110I CYCGRG 2 cut(s) 965, 1347
BmgT120I GGNCC 1 cut(s) 1192
BmiI GGNNCC 4 cut(s) 729, 817, 884, 1193
BmrFI CCNGG 5 cut(s) 878, 924, 1113, 1348, 1349
BmsI GCATC 3 cut(s) 262, 593, 1324
BmtI GCTAGC 1 cut(s) 1028
BpiI GAAGAC 1 cut(s) 360
BpuEI CTTGAG 3 cut(s) 1037, 1054, 1240
BpuMI CCSGG 2 cut(s) 1348, 1349
Bsa29I ATCGAT 1 cut(s) 998
BsaJI CCNNGG 8 cut(s) 120, 447, 518, 877, 1112, 1346, 1347, 1479
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 613, 772
Bse1I ACTGG 1 cut(s) 1512
Bse21I CCTNAGG 1 cut(s) 691
Bse3DI GCAATG 1 cut(s) 79
BseBI CCWGG 3 cut(s) 878, 924, 1113
BseCI ATCGAT 1 cut(s) 998
BseDI CCNNGG 8 cut(s) 120, 447, 518, 877, 1112, 1346, 1347, 1479
BseGI GGATG 7 cut(s) 202, 206, 403, 964, 1212, 1371, 1479
BseLI CCNNNNNNNGG 3 cut(s) 274, 613, 772
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 2 cut(s) 144, 705
BseNI ACTGG 1 cut(s) 1512
BseRI GAGGAG 1 cut(s) 1589
BseSI GKGCMC 1 cut(s) 559
BseXI GCAGC 9 cut(s) 81, 501, 522, 544, 686, 764, 818, 1091, 1349
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 5 cut(s) 16, 669, 993, 1111, 1225
BshVI ATCGAT 1 cut(s) 998
BsiHKAI GWGCWC 5 cut(s) 27, 331, 559, 606, 1511
BsiHKCI CYCGRG 2 cut(s) 965, 1347
BsiSI CCGG 2 cut(s) 216, 1348
BslFI GGGAC 2 cut(s) 1205, 1409
BslI CCNNNNNNNGG 3 cut(s) 274, 613, 772
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 1205, 1409
BsnI GGCC 5 cut(s) 16, 669, 993, 1111, 1225
BsoBI CYCGRG 2 cut(s) 965, 1347
Bsp1286I GDGCHC 6 cut(s) 27, 331, 559, 606, 1511, 1588
Bsp143I GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 4 cut(s) 275, 650, 1441, 1580
BspANI GGCC 5 cut(s) 16, 669, 993, 1111, 1225
BspCNI CTCAG 2 cut(s) 145, 704
BspDI ATCGAT 1 cut(s) 998
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 4 cut(s) 729, 817, 884, 1193
BspMAI CTGCAG 1 cut(s) 1109
BspOI GCTAGC 1 cut(s) 1028
BspPI GGATC 5 cut(s) 134, 407, 626, 956, 1542
BspQI GCTCTTC 2 cut(s) 1208, 1593
BsrBI CCGCTC 1 cut(s) 1441
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 1 cut(s) 1512
BssECI CCNNGG 8 cut(s) 120, 447, 518, 877, 1112, 1346, 1347, 1479
BssMI GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 3 cut(s) 120, 447, 1479
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 3 cut(s) 878, 924, 1113
Bst4CI ACNGT 5 cut(s) 973, 1180, 1240, 1548, 1553
Bst6I CTCTTC 3 cut(s) 1208, 1593, 1599
BstC8I GCNNGC 7 cut(s) 18, 478, 832, 929, 1026, 1109, 1417
BstDEI CTNAG 4 cut(s) 62, 153, 691, 1538
BstDSI CCRYGG 2 cut(s) 518, 1479
BstENI CCTNNNNNAGG 1 cut(s) 770
BstF5I GGATG 7 cut(s) 202, 206, 403, 964, 1212, 1371, 1479
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 2 cut(s) 152, 551
BstKTI GATC 6 cut(s) 142, 415, 634, 964, 1002, 1537
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
BstMWI GCNNNNNNNGC 6 cut(s) 22, 486, 683, 815, 928, 1021
BstNI CCWGG 3 cut(s) 878, 924, 1113
BstNSI RCATGY 2 cut(s) 834, 1159
BstSCI CCNGG 5 cut(s) 876, 922, 1111, 1346, 1347
BstSFI CTRYAG 2 cut(s) 145, 1105
BstSLI GKGCMC 1 cut(s) 559
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 9 cut(s) 81, 501, 522, 544, 686, 764, 818, 1091, 1349
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 631, 1534
BstYI RGATCY 2 cut(s) 631, 1534
Bsu15I ATCGAT 1 cut(s) 998
Bsu36I CCTNAGG 1 cut(s) 691
BsuRI GGCC 5 cut(s) 16, 669, 993, 1111, 1225
BsuTUI ATCGAT 1 cut(s) 998
BtgI CCRYGG 2 cut(s) 518, 1479
BtsCI GGATG 7 cut(s) 202, 206, 403, 964, 1212, 1371, 1479
BtsI GCAGTG 1 cut(s) 1388
BtsIMutI CAGTG 3 cut(s) 1388, 1519, 1558
Cac8I GCNNGC 7 cut(s) 18, 478, 832, 929, 1026, 1109, 1417
CaiI CAGNNNCTG 1 cut(s) 1387
CfoI GCGC 2 cut(s) 152, 551
Cfr13I GGNCC 1 cut(s) 1192
Cfr9I CCCGGG 1 cut(s) 1347
ClaI ATCGAT 1 cut(s) 998
CseI GACGC 1 cut(s) 968
Csp6I GTAC 5 cut(s) 37, 169, 483, 1549, 1561
CspCI CAANNNNNGTGG 2 cut(s) 921, 956
CviQI GTAC 5 cut(s) 37, 169, 483, 1549, 1561
DdeI CTNAG 4 cut(s) 62, 153, 691, 1538
DpnI GATC 6 cut(s) 141, 414, 633, 963, 1001, 1536
DpnII GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
DraI TTTAAA 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 14
Eam1104I CTCTTC 3 cut(s) 1208, 1593, 1599
EarI CTCTTC 3 cut(s) 1208, 1593, 1599
Ecl136II GAGCTC 2 cut(s) 25, 604
Eco130I CCWWGG 3 cut(s) 120, 447, 1479
Eco147I AGGCCT 3 cut(s) 669, 993, 1111
Eco24I GRGCYC 3 cut(s) 27, 606, 1588
Eco47I GGWCC 1 cut(s) 1192
Eco47III AGCGCT 1 cut(s) 151
Eco53kI GAGCTC 2 cut(s) 25, 604
Eco57I CTGAAG 1 cut(s) 1611
Eco81I CCTNAGG 1 cut(s) 691
Eco88I CYCGRG 2 cut(s) 965, 1347
EcoICRI GAGCTC 2 cut(s) 25, 604
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoRII CCWGG 3 cut(s) 876, 922, 1111
EcoT14I CCWWGG 3 cut(s) 120, 447, 1479
EcoT38I GRGCYC 3 cut(s) 27, 606, 1588
ErhI CCWWGG 3 cut(s) 120, 447, 1479
FaqI GGGAC 2 cut(s) 1205, 1409
FauI CCCGC 1 cut(s) 1573
FokI GGATG 7 cut(s) 189, 193, 410, 971, 1219, 1378, 1486
FriOI GRGCYC 3 cut(s) 27, 606, 1588
FspBI CTAG 2 cut(s) 671, 1025
GlaI GCGC 2 cut(s) 151, 550
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 5 cut(s) 16, 669, 993, 1111, 1225
HapII CCGG 2 cut(s) 216, 1348
HgaI GACGC 1 cut(s) 968
HhaI GCGC 2 cut(s) 152, 551
Hin6I GCGC 2 cut(s) 150, 549
HinP1I GCGC 2 cut(s) 150, 549
HincII GTYRAC 4 cut(s) 93, 948, 1132, 1183
HindII GTYRAC 4 cut(s) 93, 948, 1132, 1183
HindIII AAGCTT 1 cut(s) 308
HpaI GTTAAC 3 cut(s) 948, 1132, 1183
HpaII CCGG 2 cut(s) 216, 1348
HphI GGTGA 5 cut(s) 413, 435, 1142, 1222, 1579
Hpy166II GTNNAC 7 cut(s) 93, 557, 853, 948, 1132, 1183, 1405
Hpy188I TCNGA 6 cut(s) 112, 136, 466, 565, 608, 1206
Hpy188III TCNNGA 7 cut(s) 314, 421, 693, 965, 1331, 1358, 1532
Hpy8I GTNNAC 7 cut(s) 93, 557, 853, 948, 1132, 1183, 1405
HpyAV CCTTC 3 cut(s) 659, 1415, 1564
HpyCH4III ACNGT 5 cut(s) 973, 1180, 1240, 1548, 1553
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 486, 683, 815, 928, 1021
HpyF3I CTNAG 4 cut(s) 62, 153, 691, 1538
HspAI GCGC 2 cut(s) 150, 549
KspAI GTTAAC 3 cut(s) 948, 1132, 1183
Kzo9I GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
LguI GCTCTTC 2 cut(s) 1208, 1593
LmnI GCTCC 4 cut(s) 218, 438, 609, 815
Lsp1109I GCAGC 9 cut(s) 81, 501, 522, 544, 686, 764, 818, 1091, 1349
LweI GCATC 3 cut(s) 262, 593, 1324
MaeI CTAG 2 cut(s) 671, 1025
MaeIII GTNAC 3 cut(s) 196, 1234, 1516
MalI GATC 6 cut(s) 141, 414, 633, 963, 1001, 1536
MbiI CCGCTC 1 cut(s) 1441
MboI GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
MboII GAAGA 8 cut(s) 203, 302, 365, 590, 1193, 1225, 1580, 1616
MflI RGATCY 2 cut(s) 631, 1534
MhlI GDGCHC 6 cut(s) 27, 331, 559, 606, 1511, 1588
MluCI AATT 8 cut(s) 49, 317, 370, 428, 621, 698, 791, 1617
MlyI GAGTC 7 cut(s) 173, 268, 434, 461, 461, 925, 1363
MmeI TCCRAC 2 cut(s) 631, 967
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 6 cut(s) 336, 789, 947, 1131, 1182, 1616
MslI CAYNNNNRTG 2 cut(s) 392, 1514
MspA1I CMGCKG 1 cut(s) 535
MspI CCGG 2 cut(s) 216, 1348
MspR9I CCNGG 5 cut(s) 878, 924, 1113, 1348, 1349
MvaI CCWGG 3 cut(s) 878, 924, 1113
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 6 cut(s) 22, 486, 683, 815, 928, 1021
NciI CCSGG 2 cut(s) 1348, 1349
NcoI CCATGG 1 cut(s) 1479
NdeII GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
NheI GCTAGC 1 cut(s) 1024
NlaIV GGNNCC 4 cut(s) 729, 817, 884, 1193
NmuCI GTSAC 1 cut(s) 1516
NspI RCATGY 2 cut(s) 834, 1159
OliI CACNNNNGTG 1 cut(s) 1514
PaeI GCATGC 1 cut(s) 834
PceI AGGCCT 3 cut(s) 669, 993, 1111
PciSI GCTCTTC 2 cut(s) 1208, 1593
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 1325, 1523, 1528
PleI GAGTC 7 cut(s) 173, 267, 434, 460, 461, 924, 1362
PpsI GAGTC 7 cut(s) 173, 267, 434, 460, 461, 924, 1362
Psp124BI GAGCTC 2 cut(s) 27, 606
Psp6I CCWGG 3 cut(s) 876, 922, 1111
PspGI CCWGG 3 cut(s) 876, 922, 1111
PspN4I GGNNCC 4 cut(s) 729, 817, 884, 1193
PspPI GGNCC 1 cut(s) 1192
PstI CTGCAG 1 cut(s) 1109
PstNI CAGNNNCTG 1 cut(s) 1387
PsuI RGATCY 2 cut(s) 631, 1534
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 5 cut(s) 38, 170, 484, 1550, 1562
RsaNI GTAC 5 cut(s) 37, 169, 483, 1549, 1561
RseI CAYNNNNRTG 2 cut(s) 392, 1514
SacI GAGCTC 2 cut(s) 27, 606
SapI GCTCTTC 2 cut(s) 1208, 1593
SaqAI TTAA 6 cut(s) 336, 789, 947, 1131, 1182, 1616
Sau3AI GATC 6 cut(s) 139, 412, 631, 961, 999, 1534
Sau96I GGNCC 1 cut(s) 1192
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 7 cut(s) 173, 268, 434, 461, 461, 925, 1363
ScrFI CCNGG 5 cut(s) 878, 924, 1113, 1348, 1349
SduI GDGCHC 6 cut(s) 27, 331, 559, 606, 1511, 1588
SfaNI GCATC 3 cut(s) 262, 593, 1324
SfcI CTRYAG 2 cut(s) 145, 1105
SinI GGWCC 1 cut(s) 1192
SmaI CCCGGG 1 cut(s) 1349
SmiMI CAYNNNNRTG 2 cut(s) 392, 1514
SmlI CTYRAG 3 cut(s) 1016, 1069, 1219
SmoI CTYRAG 3 cut(s) 1016, 1069, 1219
SphI GCATGC 1 cut(s) 834
Sse9I AATT 8 cut(s) 49, 317, 370, 428, 621, 698, 791, 1617
SseBI AGGCCT 3 cut(s) 669, 993, 1111
SsiI CCGC 4 cut(s) 275, 650, 1441, 1580
SspI AATATT 1 cut(s) 102
SspMI CTAG 2 cut(s) 671, 1025
SstI GAGCTC 2 cut(s) 27, 606
StuI AGGCCT 3 cut(s) 669, 993, 1111
StyD4I CCNGG 5 cut(s) 876, 922, 1111, 1346, 1347
StyI CCWWGG 3 cut(s) 120, 447, 1479
TaaI ACNGT 5 cut(s) 973, 1180, 1240, 1548, 1553
TaqI TCGA 6 cut(s) 576, 914, 998, 1050, 1374, 1526
TasI AATT 8 cut(s) 49, 317, 370, 428, 621, 698, 791, 1617
TatI WGTACW 2 cut(s) 168, 1548
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 3 cut(s) 1325, 1523, 1528
Tru1I TTAA 6 cut(s) 336, 789, 947, 1131, 1182, 1616
Tru9I TTAA 6 cut(s) 336, 789, 947, 1131, 1182, 1616
TscAI CASTG 3 cut(s) 1388, 1519, 1558
TseFI GTSAC 1 cut(s) 1516
TseI GCWGC 9 cut(s) 69, 489, 532, 535, 674, 752, 806, 1104, 1337
Tsp45I GTSAC 1 cut(s) 1516
TspDTI ATGAA 9 cut(s) 90, 303, 366, 441, 855, 1046, 1271, 1382, 1485
TspGWI ACGGA 2 cut(s) 535, 771
TspMI CCCGGG 1 cut(s) 1347
TspRI CASTG 3 cut(s) 1388, 1519, 1558
VneI GTGCAC 1 cut(s) 555
VpaK11BI GGWCC 1 cut(s) 1192
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 2 cut(s) 698, 791
XceI RCATGY 2 cut(s) 834, 1159
XmaI CCCGGG 1 cut(s) 1347
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 671, 1025
ZrmI AGTACT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.