Rorug06G0109900

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007910
Physical Location & Seq
Reverse (-)
14882867 .. 14883619
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0109900.1

Sequence Viewer

Length: 753 bp
ATGGACGAAGACAATGAAGCTGGTTTTGAATGCAACATTTGCCTTGCCTTAGCTGAGGACCCAGTCATAACACTCTGTGGTCATCTTTACTGTTGGTCTTGTTTGGTCAAATGGCTCTTCAATTCGCAGTATTCCAACAATGAGTGTCCGATTTGCAAGTCCGACATCGAATTCAGCAAATTAATCTTTTCTTATGGAAAGTTGAAATCGTCTGAATCCAACCCGATATCTCGGTTTGAATGTAACATTTGCTCTGGATTAGCTCAAGATCCAATTATAACACACTGTGGCCACCTCTATTGTTGGCCTTGCATCTATAAATGGCTTTCCAAGTGGAATTCAAATTCCAATTGTTGGGTTTGTAATGCTGTCATAGAGGAGAACAAATTGATCCCTCTTTATGGAAAAGGGATAAAATCGTCATCACATGATTCGGACTCAAGCTTTCGAGCTATTGCACCTGCGGGCGCTGGCAGTCTTACTTCAACTACCAAGGTGAACTGTTTTGTACGGTGTGCATTCGATGCTAGCCGGACATTGTTTCGGTTTGGAAGATATGCCATGGAGATTTTCATGACAAGGGTGTTACCAGTAGTTGCAATTCTTTTGATGTTTCATCCCTCCATGCCGAGGGAAAGGAAAAACGATTTGGTGGAGAAGGTTTTCGGGTTGAGTTTGTGGTGTTTGTTGATTCTAGTCGTTTGGGATATCCTCAAGGGAAAAATTAGACGACATCACAAATTGGAACATTGA

Protein Analysis

250

Amino Acids

28.5

Weight (kDa)

8.09

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 10 - 57 1.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 10 - 52 2.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 10 - 53 2.2e-08 Ring finger domain
zf-RING_5 PF14634 10 - 53 1.9e-07 zinc-RING finger domain
zf-C3HC4 PF00097 11 - 52 6.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 11 - 50 5.1e-09 RING-type zinc-finger
zf-C3HC4_4 PF15227 11 - 52 5e-06 zinc finger of C3HC4-type, RING
zf-C3HC4_3 PF13920 79 - 126 8.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 80 - 121 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 81 - 121 4.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 81 - 115 8.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 469
AccB7I CCANNNNNTGG 1 cut(s) 354
AciI CCGC 1 cut(s) 464
AclWI GGATC 2 cut(s) 263, 385
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 3 cut(s) 170, 337, 343
AdeI CACNNNGTG 2 cut(s) 77, 287
AfaI GTAC 1 cut(s) 510
AfiI CCNNNNNNNGG 3 cut(s) 354, 401, 630
AgsI TTSAA 6 cut(s) 29, 121, 205, 239, 342, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AluBI AGCT 5 cut(s) 20, 53, 263, 444, 452
AluI AGCT 5 cut(s) 20, 53, 263, 444, 452
AlwI GGATC 2 cut(s) 263, 385
AoxI GGCC 2 cut(s) 289, 305
ApoI RAATTY 3 cut(s) 170, 337, 343
AseI ATTAAT 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 662
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 508
AsuNHI GCTAGC 1 cut(s) 527
AvaII GGWCC 1 cut(s) 58
BalI TGGCCA 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 54
BfaI CTAG 2 cut(s) 528, 695
BfoI RGCGCY 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 469
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 60
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 321, 514
BmtI GCTAGC 1 cut(s) 531
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 15
Bpu10I CCTNAGC 2 cut(s) 49, 54
BpuEI CTTGAG 3 cut(s) 249, 424, 698
BsaJI CCNNGG 3 cut(s) 492, 561, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 401, 630
Bse1I ACTGG 2 cut(s) 62, 590
BseDI CCNNGG 3 cut(s) 492, 561, 629
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 3 cut(s) 354, 401, 630
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 62, 590
BseRI GAGGAG 1 cut(s) 392
BshFI GGCC 2 cut(s) 291, 307
BsiSI CCGG 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 354, 401, 630
BsmI GAATGC 2 cut(s) 35, 518
BsnI GGCC 2 cut(s) 291, 307
Bsp143I GATC 2 cut(s) 268, 390
Bsp19I CCATGG 1 cut(s) 561
BspACI CCGC 1 cut(s) 464
BspANI GGCC 2 cut(s) 291, 307
BspCNI CTCAG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 263, 385
BspQI GCTCTTC 1 cut(s) 122
BsrI ACTGG 2 cut(s) 62, 590
BssECI CCNNGG 3 cut(s) 492, 561, 629
BssMI GATC 2 cut(s) 268, 390
BssT1I CCWWGG 2 cut(s) 492, 561
Bst4CI ACNGT 4 cut(s) 92, 287, 503, 513
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 2 cut(s) 39, 524
BstC8I GCNNGC 3 cut(s) 466, 472, 529
BstDEI CTNAG 2 cut(s) 49, 54
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 616
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 2 cut(s) 271, 393
BstMBI GATC 2 cut(s) 268, 390
BstMWI GCNNNNNNNGC 2 cut(s) 39, 524
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 2 cut(s) 291, 307
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 1 cut(s) 616
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 3 cut(s) 466, 472, 529
CciI TCATGA 1 cut(s) 573
CfoI GCGC 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 509
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviAII CATG 4 cut(s) 428, 562, 574, 625
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 2 cut(s) 49, 54
DpnI GATC 2 cut(s) 270, 392
DpnII GATC 2 cut(s) 268, 390
DraIII CACNNNGTG 2 cut(s) 77, 287
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 492, 561
Eco32I GATATC 2 cut(s) 228, 709
Eco47I GGWCC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 58
EcoRI GAATTC 2 cut(s) 170, 337
EcoRV GATATC 2 cut(s) 228, 709
EcoT14I CCWWGG 2 cut(s) 492, 561
ErhI CCWWGG 2 cut(s) 492, 561
FaeI CATG 4 cut(s) 431, 565, 577, 628
FatI CATG 4 cut(s) 427, 561, 573, 624
FauI CCCGC 1 cut(s) 457
FokI GGATG 1 cut(s) 603
FspBI CTAG 2 cut(s) 528, 695
GlaI GCGC 1 cut(s) 469
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 2 cut(s) 291, 307
HapII CCGG 1 cut(s) 532
HhaI GCGC 1 cut(s) 470
Hin1II CATG 4 cut(s) 431, 565, 577, 628
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 4 cut(s) 215, 431, 437, 691
HpaII CCGG 1 cut(s) 532
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 150, 163, 214, 436
Hpy188III TCNNGA 3 cut(s) 255, 266, 574
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 1 cut(s) 652
HpyCH4III ACNGT 4 cut(s) 92, 287, 503, 513
HpyCH4V TGCA 6 cut(s) 33, 156, 312, 458, 518, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 524
HpyF3I CTNAG 2 cut(s) 49, 54
Hsp92II CATG 4 cut(s) 431, 565, 577, 628
HspAI GCGC 1 cut(s) 468
Kzo9I GATC 2 cut(s) 268, 390
LguI GCTCTTC 1 cut(s) 122
LpnPI CCDG 7 cut(s) 6, 75, 240, 456, 474, 545, 603
LweI GCATC 2 cut(s) 321, 514
MaeI CTAG 2 cut(s) 528, 695
MaeIII GTNAC 2 cut(s) 242, 585
MalI GATC 2 cut(s) 270, 392
MboI GATC 2 cut(s) 268, 390
MboII GAAGA 3 cut(s) 20, 109, 564
MfeI CAATTG 1 cut(s) 349
MflI RGATCY 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 291
MluNI TGGCCA 1 cut(s) 291
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 3 cut(s) 159, 186, 243
MnlI CCTC 7 cut(s) 49, 305, 370, 405, 624, 631, 722
Mox20I TGGCCA 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 662
MscI TGGCCA 1 cut(s) 291
MseI TTAA 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 291
MspI CCGG 1 cut(s) 532
MunI CAATTG 1 cut(s) 349
Mva1269I GAATGC 2 cut(s) 35, 518
MwoI GCNNNNNNNGC 2 cut(s) 39, 524
NcoI CCATGG 1 cut(s) 561
NdeII GATC 2 cut(s) 268, 390
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 4 cut(s) 431, 565, 577, 628
NlaIV GGNNCC 1 cut(s) 60
NmeAIII GCCGAG 1 cut(s) 654
PagI TCATGA 1 cut(s) 573
PaqCI CACCTGC 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 122
PctI GAATGC 2 cut(s) 35, 518
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 3 cut(s) 215, 431, 691
PflFI GACNNNGTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 354
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 58
PshBI ATTAAT 1 cut(s) 182
PsiI TTATAA 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 58
PspPPI RGGWCCY 1 cut(s) 58
PsuI RGATCY 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 182
Sau3AI GATC 2 cut(s) 268, 390
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 1 cut(s) 431
SetI ASST 9 cut(s) 22, 55, 265, 297, 446, 454, 463, 498, 663
SfaNI GCATC 2 cut(s) 321, 514
SinI GGWCC 1 cut(s) 58
SmlI CTYRAG 3 cut(s) 264, 439, 713
SmoI CTYRAG 3 cut(s) 264, 439, 713
SsiI CCGC 1 cut(s) 464
SspMI CTAG 2 cut(s) 528, 695
StyI CCWWGG 2 cut(s) 492, 561
TaaI ACNGT 4 cut(s) 92, 287, 503, 513
TaqI TCGA 3 cut(s) 168, 448, 522
TfiI GAWTC 3 cut(s) 215, 431, 691
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 1 cut(s) 290
TspDTI ATGAA 3 cut(s) 30, 562, 605
TspRI CASTG 1 cut(s) 290
Tth111I GACNNNGTC 1 cut(s) 62
Van91I CCANNNNNTGG 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 58
VspI ATTAAT 1 cut(s) 182
XapI RAATTY 3 cut(s) 170, 337, 343
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 2 cut(s) 528, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.