pycom05g07880

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016957
Physical Location & Seq
Forward (+)
10672092 .. 10675463
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07880.4

Sequence Viewer

Length: 2652 bp
ATGGCCTTGATCTTTGACTGGTCTATTTTAATGCCACGATGCTTGACAATAAAGCCGAAGAACTTCCCAGAAGTGACGCCAAATGCACACTTCAACGAGTTCATTTTGAGGTTGTACAAATCTCTTTTTGGTCTTGATAACCACGTCGTCTTCGTTGCATTCTACATTTTTCTCCAACGTTCTTTAAGCCAAAGGGCATTACCTTGTAGTAGTAGATACCTTTTGGAGTTGCTGTTAGTTCCTTGTCTTGGGGAGCCAAGCGAATTTGATTTGGTCGCATCCACCATGATTTTGATGATTGGTAAGGGAAAGTCATCCTTTGGGCAAACATCGTTGAGGTCTCAGAAGTCTACACAAATACATATTTATCCAGATTTCTTAAGGACAATGACAATGTTAGAGATCAATTTAGGGTATTGCACCTCTCGAATGAAGCCTACTTCGATTTGCTTGTCAATTTTAGCCTCAATTTGTGGGATGAGTTCCAGGCTTGACATCAAGATGATGCATGGTGATGGTAGGGTCAAGCCTGGGCTTTTCTTTGTAGGTCCAAGCAAAGACGTCTTTGTACCCCATTACCAGTTGGTAGTACTCCTTGATCTTGTCCTTGAGCTCCTTGATCGATCATCAACCGTGGCTTGCTCTTTATCCTTGAGCTATGGTGGCGCAGTAGTGACATCTTCCTCAGGGAATTTGTCTTCTTTGTCTTTTTGGATTGTGATGTGGAAGACGTCTTTGACCTCTTTTTTAGTGCCGTCCTCTTGGGCTTGTTGGCATGAAGATTGGCCACTGTGGATGGTGATGCACTTATTTACCTTCAATTGTCCTTTTGTGTCAACTTCCAAGGTTGCTTGGCGCTTTATCCTTGAAGGAATCAAGATTCGAACGTTGGTTTTTTCGACTAGATATGGTTTTCCGGTTTCTTCAAGTGTTTCCAAGGTCGACTGTCATGGAGGAGAGCTAGCGGTGTTGCTTTGTTTCTTAGGTTTTGACAACATTTTGAAAACAGAACTCTTGGGTGTTGGCGTGTTATGCCATTTGAAGACGGAGGTTTGTTTGAGGGGTGGCTTTAGGCTTCTCTTCACTTTGTTCAACACTCAAGCTGATGTGTTGAATTTGGTGGGAAGCCAAGTCCAGTTTTGTTGTTGTCAACACCGTAACCATGCTCTTTCAACTTATTTTGAGTCTCAGAAAGGTCATGTTTTTGTTGTTGACGGTATTGGAAACCTTCTTTCCAAGAATTTAGGGGTCAGAACCTTCTTCGATCCTCTTGGTTGGAAGAGAGCTTGGTTCTGCCCCCTTTGGCAGGGGTTTTACGAAACTGGTGGCTTTGAAACTTTAGCATCGCCTAGCTGTGTCAGAGGTAAAACTGTGTTTGTCTTGAGTAGCTTCACATCGTCCTTGTGCAGCCGTGTGTTTGCTTTGCTTACATTGGGGGCGTATCAAAAGACAGATGTGTTTGGTCATTTTGAGGGCATCACACTTCCTTTGGTTGTTGTGGGTTTAGCAGGCTTATCATCATTTTTGCCTGAAGATGGCATAGCTTTCCCTTCTTGCTTCTTGGGCACGACTTGCCACTCTTGTTTCTTAGGTGCGGCTTTGCCTGTTGATTTGATTTCTTTTGGAAGAGCTTCGGGTACTATGTCTTCATCCATTTTAAGCATTAGTGGAGGACAGACGACACTACTCCATTCTCGTGGATCCAAGAGCTTCCTAAAAGTAAGCTATAGGAAGTTCTTGCATCAATCACATGGAAATAATGTGCTTGATTTGAGTTTGCCAATGACCATCTCGACTCGGATCATGCTCATTGCTTTTTGTCCTCAATTGTTAAAACCTTGGATTATGAGGGGGCTTAGGGATAGTACATCCACCTTGATGCCGATTGTAGCCATTGTTGACTTTGGCATGATGTTTATGGCCCATCCACTATCAACTAGCATGCGGTTGACTTTGTGTTCCCTCACGTACCTAGAGTCGAATAGAGGATGGTTGGAAGGCTTTGACCCTAGCAGCAAGTCTTCATCAGTGAAGTTGATTGTCTTCAAGCCTTCGTCCTTGCTTTCTTGCACCTCGTGGTCGTCGGGACTCGCCAAGACTGCTACTAGTGCTTTTCGTATCTTCTTAGGCAATCGTAATGCTTCCTCGATGCTAAAGTGTGCCAGCAGGCTTTCTTTGGTGGTAGTGCACTATTGGAAGAAGTCATTCGGGAAGTACTCGTGCAAGAAGATGAGAATGCATAGCTCTTGCTCCAGAGGTTCCCCAACATACATTAGTTTAGCAGCTTTTACGCTCATTTTCGGCTTCCTATTATTGCAACGGTGCTTTCTCCCTTGTTCCACCTTTGGCTGTGTGGCTTGTGGTCTTGGCTGCCTTGTCTATTTAAGGTGCCGTGTAGCTTATGCATGGACGGGAGTGGTCATGTGTTGCTTGGAGAGGCACAGGATCGAAAGATCCAAACACGATTGTAGTAGTGTGTGTTGCGTCCATGTCCTCAAGGTCAAGTTCGATTTACCATTGCTGTGCCAACTTCATGATGAGCTCCTTGAAGACGAAGTATTTTCTAACCGGATAACTCACGATACAATGGTATGTGCAGTACCTAGGATCGTTAACGCAATTCATCTTTTCAAAACGGTTGCATTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

884

Amino Acids

98.21

Weight (kDa)

9.12

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 564, 734
AccB1I GGYRCC 1 cut(s) 2388
AccI GTMKAC 2 cut(s) 350, 942
AciI CCGC 3 cut(s) 965, 1595, 1945
AclI AACGTT 2 cut(s) 178, 887
AclWI GGATC 7 cut(s) 1259, 1695, 1708, 1808, 2448, 2453, 2615
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 4 cut(s) 263, 691, 1114, 1240
AcuI CTGAAG 1 cut(s) 1551
AcyI GRCGYC 3 cut(s) 77, 561, 731
AdeI CACNNNGTG 1 cut(s) 2076
AfaI GTAC 8 cut(s) 116, 570, 591, 1639, 1867, 1970, 2216, 2601
AfiI CCNNNNNNNGG 3 cut(s) 248, 1306, 1535
AflII CTTAAG 1 cut(s) 379
AhlI ACTAGT 1 cut(s) 2105
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 485, 529
Alw21I GWGCWC 3 cut(s) 615, 2190, 2544
Alw26I GTCTC 2 cut(s) 345, 1191
Alw44I GTGCAC 1 cut(s) 2186
AlwI GGATC 7 cut(s) 1259, 1695, 1708, 1808, 2448, 2453, 2615
AoxI GGCC 3 cut(s) 3, 785, 1920
ApaLI GTGCAC 1 cut(s) 2186
ApeKI GCWGC 4 cut(s) 1407, 2013, 2282, 2370
ApoI RAATTY 4 cut(s) 263, 691, 1114, 1240
Asp700I GAANNNNTTC 3 cut(s) 62, 1630, 2204
AspA2I CCTAGG 1 cut(s) 2603
AspLEI GCGC 2 cut(s) 668, 858
AspS9I GGNCC 2 cut(s) 548, 1921
AsuHPI GGTGA 2 cut(s) 524, 811
AsuII TTCGAA 1 cut(s) 883
AsuNHI GCTAGC 1 cut(s) 961
AvaII GGWCC 1 cut(s) 548
AvrII CCTAGG 1 cut(s) 2603
AxyI CCTNAGG 1 cut(s) 685
BaeGI GKGCMC 2 cut(s) 1568, 2190
BaeI ACNNNNGTAYC 2 cut(s) 2574, 2607
BalI TGGCCA 1 cut(s) 787
BamHI GGATCC 1 cut(s) 1700
BanI GGYRCC 1 cut(s) 2388
BanII GRGCYC 2 cut(s) 615, 2544
BarI GAAGNNNNNNTAC 2 cut(s) 1630, 1662
BauI CACGAG 3 cut(s) 1695, 2074, 2218
BbsI GAAGAC 8 cut(s) 142, 690, 734, 1049, 1638, 2013, 2035, 2556
Bbv12I GWGCWC 3 cut(s) 615, 2190, 2544
BbvI GCAGC 4 cut(s) 1419, 2025, 2294, 2357
BccI CCATC 6 cut(s) 509, 790, 1529, 1796, 1932, 1983
BceAI ACGGC 3 cut(s) 739, 1395, 2376
BciT130I CCWGG 2 cut(s) 487, 531
BcoDI GTCTC 2 cut(s) 345, 1191
BcuI ACTAGT 1 cut(s) 2105
BfaI CTAG 8 cut(s) 903, 962, 1350, 1938, 1973, 2010, 2106, 2604
BfmI CTRYAG 1 cut(s) 1726
BfoI RGCGCY 1 cut(s) 859
BfrI CTTAAG 1 cut(s) 379
BisI GCNGC 5 cut(s) 1408, 1596, 2014, 2283, 2371
BlnI CCTAGG 1 cut(s) 2603
BlsI GCNGC 5 cut(s) 1409, 1597, 2015, 2284, 2372
BmcAI AGTACT 2 cut(s) 591, 2216
Bme1390I CCNGG 2 cut(s) 487, 531
Bme18I GGWCC 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 548, 1921
BmiI GGNNCC 4 cut(s) 255, 1702, 2260, 2390
BmrFI CCNGG 2 cut(s) 487, 531
BmsI GCATC 9 cut(s) 29, 287, 495, 792, 1352, 1485, 1750, 1869, 2139
BmtI GCTAGC 1 cut(s) 965
BpiI GAAGAC 8 cut(s) 142, 690, 734, 1049, 1638, 2013, 2035, 2556
BpmI CTGGAG 1 cut(s) 2236
Bpu10I CCTNAGC 1 cut(s) 1856
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 5 cut(s) 629, 673, 1083, 1402, 2480
Bsa29I ATCGAT 1 cut(s) 622
BsaAI YACGTR 1 cut(s) 1968
BsaHI GRCGYC 3 cut(s) 77, 561, 731
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 6 cut(s) 530, 633, 843, 936, 1838, 2603
BsaWI WCCGGW 2 cut(s) 916, 2568
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 1306, 1535
Bse1I ACTGG 4 cut(s) 23, 580, 1135, 1327
Bse21I CCTNAGG 1 cut(s) 685
Bse3DI GCAATG 2 cut(s) 1809, 2516
BseBI CCWGG 2 cut(s) 487, 531
BseCI ATCGAT 1 cut(s) 622
BseDI CCNNGG 6 cut(s) 530, 633, 843, 936, 1838, 2603
BseGI GGATG 8 cut(s) 278, 314, 483, 801, 1649, 1868, 1924, 1994
BseLI CCNNNNNNNGG 3 cut(s) 248, 1306, 1535
BseMI GCAATG 2 cut(s) 1809, 2516
BseMII CTCAG 3 cut(s) 356, 699, 1202
BseNI ACTGG 4 cut(s) 23, 580, 1135, 1327
BseRI GAGGAG 1 cut(s) 969
BseSI GKGCMC 2 cut(s) 1568, 2190
BseXI GCAGC 4 cut(s) 1419, 2025, 2294, 2357
BsgI GTGCAG 2 cut(s) 1426, 2616
BshFI GGCC 3 cut(s) 5, 787, 1922
BshNI GGYRCC 1 cut(s) 2388
BshVI ATCGAT 1 cut(s) 622
BsiHKAI GWGCWC 3 cut(s) 615, 2190, 2544
BsiSI CCGG 2 cut(s) 917, 2569
BslFI GGGAC 1 cut(s) 2100
BslI CCNNNNNNNGG 3 cut(s) 248, 1306, 1535
BsmAI GTCTC 2 cut(s) 345, 1191
BsmFI GGGAC 1 cut(s) 2100
BsmI GAATGC 2 cut(s) 158, 2241
BsnI GGCC 3 cut(s) 5, 787, 1922
Bso31I GGTCTC 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 4 cut(s) 615, 1568, 2190, 2544
Bsp1407I TGTACA 1 cut(s) 114
BspACI CCGC 3 cut(s) 965, 1595, 1945
BspANI GGCC 3 cut(s) 5, 787, 1922
BspCNI CTCAG 3 cut(s) 355, 698, 1201
BspDI ATCGAT 1 cut(s) 622
BspHI TCATGA 1 cut(s) 2533
BspLI GGNNCC 4 cut(s) 255, 1702, 2260, 2390
BspOI GCTAGC 1 cut(s) 965
BspPI GGATC 7 cut(s) 1259, 1695, 1708, 1808, 2448, 2453, 2615
BspQI GCTCTTC 1 cut(s) 1621
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 2388
BspTI CTTAAG 1 cut(s) 379
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 1809, 2516
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 4 cut(s) 23, 580, 1135, 1327
BssECI CCNNGG 6 cut(s) 530, 633, 843, 936, 1838, 2603
BssNI GRCGYC 3 cut(s) 77, 561, 731
BssSI CACGAG 3 cut(s) 1695, 2074, 2218
BssT1I CCWWGG 4 cut(s) 843, 936, 1838, 2603
Bst2BI CACGAG 3 cut(s) 1695, 2074, 2218
Bst2UI CCWGG 2 cut(s) 487, 531
Bst4CI ACNGT 8 cut(s) 634, 792, 947, 1157, 1217, 1372, 2322, 2638
Bst6I CTCTTC 3 cut(s) 1085, 1274, 1621
BstACI GRCGYC 3 cut(s) 77, 561, 731
BstAFI CTTAAG 1 cut(s) 379
BstAPI GCANNNNNTGC 1 cut(s) 1572
BstAUI TGTACA 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 1968
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 6 cut(s) 640, 963, 1510, 1943, 2164, 2168
BstDEI CTNAG 7 cut(s) 342, 685, 982, 1188, 1588, 1856, 2125
BstDSI CCRYGG 1 cut(s) 633
BstENI CCTNNNNNAGG 1 cut(s) 1304
BstF5I GGATG 8 cut(s) 278, 314, 483, 801, 1649, 1868, 1924, 1994
BstH2I RGCGCY 1 cut(s) 859
BstHHI GCGC 2 cut(s) 668, 858
BstMAI GTCTC 2 cut(s) 345, 1191
BstMWI GCNNNNNNNGC 6 cut(s) 663, 1032, 1563, 1572, 2099, 2108
BstNI CCWGG 2 cut(s) 487, 531
BstNSI RCATGY 1 cut(s) 1945
BstSCI CCNGG 2 cut(s) 485, 529
BstSFI CTRYAG 1 cut(s) 1726
BstSLI GKGCMC 2 cut(s) 1568, 2190
BstV1I GCAGC 4 cut(s) 1419, 2025, 2294, 2357
BstV2I GAAGAC 8 cut(s) 142, 690, 734, 1049, 1638, 2013, 2035, 2556
BstX2I RGATCY 2 cut(s) 1700, 2453
BstXI CCANNNNNNTGG 1 cut(s) 1697
BstYI RGATCY 2 cut(s) 1700, 2453
Bsu15I ATCGAT 1 cut(s) 622
Bsu36I CCTNAGG 1 cut(s) 685
BsuRI GGCC 3 cut(s) 5, 787, 1922
BsuTUI ATCGAT 1 cut(s) 622
BtgI CCRYGG 1 cut(s) 633
BtgZI GCGATG 1 cut(s) 1329
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 8 cut(s) 278, 314, 483, 801, 1649, 1868, 1924, 1994
BtsIMutI CAGTG 2 cut(s) 788, 2034
Cac8I GCNNGC 6 cut(s) 640, 963, 1510, 1943, 2164, 2168
CciI TCATGA 1 cut(s) 2533
CfoI GCGC 2 cut(s) 668, 858
Cfr13I GGNCC 2 cut(s) 548, 1921
ClaI ATCGAT 1 cut(s) 622
CseI GACGC 2 cut(s) 85, 2473
Csp6I GTAC 8 cut(s) 115, 569, 590, 1638, 1866, 1969, 2215, 2600
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviQI GTAC 8 cut(s) 115, 569, 590, 1638, 1866, 1969, 2215, 2600
DdeI CTNAG 7 cut(s) 342, 685, 982, 1188, 1588, 1856, 2125
DraIII CACNNNGTG 1 cut(s) 2076
EaeI YGGCCR 1 cut(s) 785
Eam1104I CTCTTC 3 cut(s) 1085, 1274, 1621
EarI CTCTTC 3 cut(s) 1085, 1274, 1621
Ecl136II GAGCTC 2 cut(s) 613, 2542
Eco130I CCWWGG 4 cut(s) 843, 936, 1838, 2603
Eco24I GRGCYC 2 cut(s) 615, 2544
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 548
Eco53kI GAGCTC 2 cut(s) 613, 2542
Eco57I CTGAAG 1 cut(s) 1551
Eco81I CCTNAGG 1 cut(s) 685
EcoICRI GAGCTC 2 cut(s) 613, 2542
EcoNI CCTNNNNNAGG 1 cut(s) 1304
EcoRII CCWGG 2 cut(s) 485, 529
EcoT14I CCWWGG 4 cut(s) 843, 936, 1838, 2603
EcoT22I ATGCAT 3 cut(s) 510, 2241, 2407
EcoT38I GRGCYC 2 cut(s) 615, 2544
ErhI CCWWGG 4 cut(s) 843, 936, 1838, 2603
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 2100
FblI GTMKAC 2 cut(s) 350, 942
Fnu4HI GCNGC 5 cut(s) 1408, 1596, 2014, 2283, 2371
FokI GGATG 8 cut(s) 265, 301, 490, 808, 1636, 1855, 1911, 2001
FriOI GRGCYC 2 cut(s) 615, 2544
Fsp4HI GCNGC 5 cut(s) 1408, 1596, 2014, 2283, 2371
FspBI CTAG 8 cut(s) 903, 962, 1350, 1938, 1973, 2010, 2106, 2604
GlaI GCGC 2 cut(s) 667, 857
GluI GCNGC 5 cut(s) 1408, 1596, 2014, 2283, 2371
GsuI CTGGAG 1 cut(s) 2236
HaeII RGCGCY 1 cut(s) 859
HaeIII GGCC 3 cut(s) 5, 787, 1922
HapII CCGG 2 cut(s) 917, 2569
HgaI GACGC 2 cut(s) 85, 2473
HhaI GCGC 2 cut(s) 668, 858
Hin1I GRCGYC 3 cut(s) 77, 561, 731
Hin6I GCGC 2 cut(s) 666, 856
HinP1I GCGC 2 cut(s) 666, 856
HincII GTYRAC 7 cut(s) 837, 943, 1151, 1213, 1900, 1950, 2614
HindII GTYRAC 7 cut(s) 837, 943, 1151, 1213, 1900, 1950, 2614
HinfI GANTC 6 cut(s) 873, 880, 1184, 1795, 1976, 2088
HpaI GTTAAC 1 cut(s) 2614
HpaII CCGG 2 cut(s) 917, 2569
HphI GGTGA 2 cut(s) 524, 811
Hpy166II GTNNAC 9 cut(s) 351, 837, 943, 1151, 1213, 1900, 1950, 2188, 2614
Hpy188I TCNGA 5 cut(s) 345, 1191, 1253, 1361, 1800
Hpy8I GTNNAC 9 cut(s) 351, 837, 943, 1151, 1213, 1900, 1950, 2188, 2614
Hpy99I CGWCG 2 cut(s) 149, 2086
HpyAV CCTTC 7 cut(s) 826, 863, 1238, 1267, 1560, 1991, 2061
HpyCH4III ACNGT 8 cut(s) 634, 792, 947, 1157, 1217, 1372, 2322, 2638
HpyCH4IV ACGT 6 cut(s) 144, 178, 561, 731, 887, 1967
HpyF10VI GCNNNNNNNGC 6 cut(s) 663, 1032, 1563, 1572, 2099, 2108
HpyF3I CTNAG 7 cut(s) 342, 685, 982, 1188, 1588, 1856, 2125
HpySE526I ACGT 6 cut(s) 144, 178, 561, 731, 887, 1967
Hsp92I GRCGYC 3 cut(s) 77, 561, 731
HspAI GCGC 2 cut(s) 666, 856
KspAI GTTAAC 1 cut(s) 2614
LguI GCTCTTC 1 cut(s) 1621
LmnI GCTCC 4 cut(s) 253, 618, 2255, 2547
Lsp1109I GCAGC 4 cut(s) 1419, 2025, 2294, 2357
LweI GCATC 9 cut(s) 29, 287, 495, 792, 1352, 1485, 1750, 1869, 2139
MaeI CTAG 8 cut(s) 903, 962, 1350, 1938, 1973, 2010, 2106, 2604
MaeII ACGT 6 cut(s) 144, 178, 561, 731, 887, 1967
MaeIII GTNAC 3 cut(s) 73, 673, 1157
MfeI CAATTG 2 cut(s) 820, 1826
MflI RGATCY 2 cut(s) 1700, 2453
MhlI GDGCHC 4 cut(s) 615, 1568, 2190, 2544
MlsI TGGCCA 1 cut(s) 787
MluNI TGGCCA 1 cut(s) 787
MlyI GAGTC 4 cut(s) 1193, 1789, 1985, 2082
MmeI TCCRAC 3 cut(s) 199, 1256, 1974
Mox20I TGGCCA 1 cut(s) 787
Mph1103I ATGCAT 3 cut(s) 510, 2241, 2407
MroXI GAANNNNTTC 3 cut(s) 62, 1630, 2204
MscI TGGCCA 1 cut(s) 787
MseI TTAA 7 cut(s) 29, 185, 380, 1658, 1832, 2384, 2613
MslI CAYNNNNRTG 5 cut(s) 500, 513, 1695, 1877, 2521
Msp20I TGGCCA 1 cut(s) 787
MspCI CTTAAG 1 cut(s) 379
MspI CCGG 2 cut(s) 917, 2569
MspR9I CCNGG 2 cut(s) 487, 531
MunI CAATTG 2 cut(s) 820, 1826
Mva1269I GAATGC 2 cut(s) 158, 2241
MvaI CCWGG 2 cut(s) 487, 531
MwoI GCNNNNNNNGC 6 cut(s) 663, 1032, 1563, 1572, 2099, 2108
NheI GCTAGC 1 cut(s) 961
NlaIV GGNNCC 4 cut(s) 255, 1702, 2260, 2390
NmuCI GTSAC 2 cut(s) 73, 673
NsiI ATGCAT 3 cut(s) 510, 2241, 2407
NspI RCATGY 1 cut(s) 1945
NspV TTCGAA 1 cut(s) 883
PaeI GCATGC 1 cut(s) 1945
PagI TCATGA 1 cut(s) 2533
PciSI GCTCTTC 1 cut(s) 1621
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 2 cut(s) 158, 2241
PdmI GAANNNNTTC 3 cut(s) 62, 1630, 2204
PfeI GAWTC 2 cut(s) 873, 880
PkrI GCNGC 5 cut(s) 1409, 1597, 2015, 2284, 2372
PleI GAGTC 4 cut(s) 1192, 1789, 1984, 2082
PpsI GAGTC 4 cut(s) 1192, 1789, 1984, 2082
Ppu21I YACGTR 1 cut(s) 1968
Psp124BI GAGCTC 2 cut(s) 615, 2544
Psp1406I AACGTT 2 cut(s) 178, 887
Psp6I CCWGG 2 cut(s) 485, 529
PspGI CCWGG 2 cut(s) 485, 529
PspN4I GGNNCC 4 cut(s) 255, 1702, 2260, 2390
PspPI GGNCC 2 cut(s) 548, 1921
PsuI RGATCY 2 cut(s) 1700, 2453
RsaI GTAC 8 cut(s) 116, 570, 591, 1639, 1867, 1970, 2216, 2601
RsaNI GTAC 8 cut(s) 115, 569, 590, 1638, 1866, 1969, 2215, 2600
RseI CAYNNNNRTG 5 cut(s) 500, 513, 1695, 1877, 2521
SacI GAGCTC 2 cut(s) 615, 2544
SalI GTCGAC 1 cut(s) 941
SapI GCTCTTC 1 cut(s) 1621
SaqAI TTAA 7 cut(s) 29, 185, 380, 1658, 1832, 2384, 2613
SatI GCNGC 5 cut(s) 1408, 1596, 2014, 2283, 2371
Sau96I GGNCC 2 cut(s) 548, 1921
ScaI AGTACT 2 cut(s) 591, 2216
SchI GAGTC 4 cut(s) 1193, 1789, 1985, 2082
ScrFI CCNGG 2 cut(s) 487, 531
SduI GDGCHC 4 cut(s) 615, 1568, 2190, 2544
SfaNI GCATC 9 cut(s) 29, 287, 495, 792, 1352, 1485, 1750, 1869, 2139
SfcI CTRYAG 1 cut(s) 1726
SfuI TTCGAA 1 cut(s) 883
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 5 cut(s) 500, 513, 1695, 1877, 2521
SmlI CTYRAG 6 cut(s) 379, 608, 652, 1098, 1381, 2495
SmoI CTYRAG 6 cut(s) 379, 608, 652, 1098, 1381, 2495
SpeI ACTAGT 1 cut(s) 2105
SphI GCATGC 1 cut(s) 1945
SsiI CCGC 3 cut(s) 965, 1595, 1945
SspMI CTAG 8 cut(s) 903, 962, 1350, 1938, 1973, 2010, 2106, 2604
SstI GAGCTC 2 cut(s) 615, 2544
StyD4I CCNGG 2 cut(s) 485, 529
StyI CCWWGG 4 cut(s) 843, 936, 1838, 2603
TaaI ACNGT 8 cut(s) 634, 792, 947, 1157, 1217, 1372, 2322, 2638
TaiI ACGT 6 cut(s) 147, 181, 564, 734, 890, 1970
TatI WGTACW 4 cut(s) 114, 589, 1865, 2214
TauI GCSGC 1 cut(s) 1598
TfiI GAWTC 2 cut(s) 873, 880
Tru1I TTAA 7 cut(s) 29, 185, 380, 1658, 1832, 2384, 2613
Tru9I TTAA 7 cut(s) 29, 185, 380, 1658, 1832, 2384, 2613
TscAI CASTG 2 cut(s) 795, 2034
TseFI GTSAC 2 cut(s) 73, 673
TseI GCWGC 4 cut(s) 1407, 2013, 2282, 2370
Tsp45I GTSAC 2 cut(s) 73, 673
TspDTI ATGAA 7 cut(s) 91, 446, 792, 1638, 2013, 2522, 2612
TspGWI ACGGA 1 cut(s) 1061
TspRI CASTG 2 cut(s) 795, 2034
Vha464I CTTAAG 1 cut(s) 379
VneI GTGCAC 1 cut(s) 2186
VpaK11BI GGWCC 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 1304
XapI RAATTY 4 cut(s) 263, 691, 1114, 1240
XceI RCATGY 1 cut(s) 1945
XmaJI CCTAGG 1 cut(s) 2603
XmiI GTMKAC 2 cut(s) 350, 942
XmnI GAANNNNTTC 3 cut(s) 62, 1630, 2204
XspI CTAG 8 cut(s) 903, 962, 1350, 1938, 1973, 2010, 2106, 2604
ZraI GACGTC 2 cut(s) 562, 732
ZrmI AGTACT 2 cut(s) 591, 2216
Zsp2I ATGCAT 3 cut(s) 510, 2241, 2407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.