pycom13g28330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016957
Physical Location & Seq
Forward (+)
24202148 .. 24206308
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28330.10

Sequence Viewer

Length: 3027 bp
ATGAAGAGGTTCCTGGGTTGTTTTGATGATGTAGAATGGATTTTGGGGGGTGTAGAGGCACGGCAAGGGTGTAGGTTGAGTGTGGATGAGTGTTTGATGGTTGGAAGGTGGTGGAGAACTCGGCAAAGAGGGTGGAAGGAGGTGGAGCGGCTGCTTGGTGTGAATGGACGAATTTCTGTGATGAATGATGGATGGCTGCCTAGGTTATGTGTTTTTTTTGGGGTGCGGCTGCTTAAGGTTCATATATATATACAGATACAAGTGATTAGGGCCCTTAGGGCTTGCGGCAAAGGTTGGGAACTTAAGTCCTATAGAAATAGGTTTAAGAATTTTCGTCCAAAATTGGGCCTTCTAGAAACTAAGTGTCTTATGGCTGATTTTGGGTATTCTAGAAGGCATAAAAGGGGGGGAAGGTGTGGCACCCTTTTAGGGTGGAACAAGATAAGGTTAGGACAAGGTTTCCTCTTTTCTTGCACTTTCCTTATCTTCTTTGTCATGAACTTTCCTTTTCTGATTTGTAGCATGCTTCTAACCACTTTTGACTCCAAATTCGTCCATCCACCTTGCTCCATATACAACCTTTCGCCAAATACGTCTTATGAACCTGAAAACACACAAAAGAACACTAAAACAAGCAAACTAAGTTGCATAAATATGCTCCTATCATTGATCTTCTCTGATGTAACCATTGCCATAGGGGGTTGGAAATTTCATAAACAACATATGTGTGGATACCACAACCTTGAGATCATTAATGCATGTGATGACATTGTATGCCATTGGGCAGTCAACCACCAAGAAGTTAGTGGTGATGGTGGCTGTGTACGGGCCTGTACCGATGGTGAAGGGTACATCAAGCAAGTATTCAGCTACATTAAGTGTTTTGAAAGCTTCATCAAACATGATATTGACCGACGCCCCCGTGTCGACCAGGATTCATCGTACATCAAAGTTGGCTATGTGAGCCTCCACAATCAGTGGGTCGTTGTGAGGATAGATGATACCTCTTTCTTCCTCAGGGTCTTCCACGTGAAACACTTGGTGGCCAGGCTTCAAAGTTCGTTCATTATTTTTCATGGCCCTGTTGGAAGATTCAGATATAGGTGTGCCACCGCTTATGGAATATATCACATTCAATTGGCGTTGGTTGCGGTTATCCCTTGGAGGGTGAAGGAGAAAATGGTTAATTTTTCCTTTATGTGCCAAAGCTTCAATATGATCACGGAGGACCGTGATACATTTCTCGCCGTCATGGCCATTATACTCGTGGTAACCACAAAACATGCCTGTGTTCTTCGTGGACTTGAAATCTGGTCCTCGGCCTTGGCTTTGGTATCAAGTGAGCTATATTGGGGTAAATGGCCGTGCATGTGGCGTTCAAAGGTATGTGGGTATTATTGTGTGATACCTTTGGTTATCGTGGTAGTGCCTCTTACTCTTTTTATTAAAATGAGACTGGTGAAGATGGAAGTCTTTCCTCTTGCCTTGAGAATGATATGCCTATTGACTTGGCGAGGTCTTCTCATTCAGTTGGATTTGGTTTCCACTCCTTATTTGTTGATAAGGGATGGTTGTAGGGAGTTTCCCTTGATATGTCATTGCCTCGGCGGAGGCATGGTTATAAGCCTGTGCCATTACCTCAGAAAACAGTTGCGTAGGCCTGCCATGAAGGCTTTAAGGGCGGTCTTGTCATTTGCCTTAGCGCAACGAGAATACTCATGGCTGAAGCGGCCGGCATACTTTCGTAGTGACTCATCCGGTTTCTGGCGAATAGTGTACAAGTCATTTAATTTCCTCAGTTCCTCAAATGAGTCTACTGTCTCAAGTGGAAGACGGCAATACCAATTCAGAGCTCCGCCAGAGAGTGTAGAGGGGAAGAGAAGACATCGTTCTTCGTCAGTGTGCATCCGGTATGCCATGGTGGACTCAAAGAGGTTAAGGTGTTCAATCGGGTCTTCCCTTCCAATGGGGGTGTCGAGGATCCTCCTTGTAAGAGGGCCAGGCCAAGGTTGGTTCCAGTTAGGACAAGGGGGTCTTGAGTGGAGTCAGGTACTGCTGGATCGATCTTCCGTAAGTCTCCATCTCCTCTTGGAAGTAGGAAAGCTTGGTCAAGGGCATGGGATTTTTCTTTGGACTTACCGTACTGACTTCCAGGGCGAGCCTATCGAAACGCCCTCGAGTCCCCCGTACCTTCATATTCCTCTAGGACATGTCGCTCATTCCCTAGATTGGCAATTGGCTTGGGGTGGATGGGGTTCTCTCGACGTTGCCTCAGGAAGTCCCGACAGTCGCGATAAACAGTTTTTGATCCTTCTACTCCTTCTGCAAGAAGGTGCCTTCCTCCATTTCTCTTGCTTCGGGTTAAAGCAGCTGGGTCGAGAGAGATCCATGTTGAAGGCAGGTGATCCCTCGTGTTGGGGGGTACCCAGGTGGTGGTTGACTTCCCCGGGGGCGATGAGTTCAGCTTCTCATACTGCTCCTGGAGGATCTCATTCTTTATCACTATCTTCAAACTTTTTTCGTTCCTTCTTTCATTGCTTTGCACTAAGTGCAAGGGGTGTCGTTCTACGTGTTGTGGCTCCCTTCACTCCCCATGTTGGAGAGGGGTGCCTGGTCGAAGGAAAGTGTGCGAATGGTGGAAACCAGCTTGACAAAGTTGGAGATAGTAGGAATAAGTGCGATTCCCACAGATGGCACCAAATGTTGATGCACAAAATTGGTGAGGACTTTGGTACAACAGAAAGTGTGAGGTTTATGACCTTCACTAGAGATAGGGAAGCAAACAACACAAGATGTACGTGGTTCACCCAGATTGGCTACGTCCACAGAGTAGAGGAGTTCTCATTAGTTGTGAAGGTTCTAGTGAATAGTTTAGTACAAATGACATTAGGGATTATTGTTGGAGATAGATCCCCTTTTATAGAAGAGATGGCCTCTGATACCGACATATGTCGTTTTGTGATTGGCCTCCTGGTTGGAGGAAAAGTCTTGTGGGTCCTTGATGGTTTGCTGTTGACCGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1009

Amino Acids

114.68

Weight (kDa)

9.44

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1622
AarI CACCTGC 1 cut(s) 2390
AasI GACNNNNNNGTC 1 cut(s) 2031
Acc36I ACCTGC 1 cut(s) 2390
Acc65I GGTACC 1 cut(s) 2422
AccB1I GGYRCC 5 cut(s) 419, 2333, 2422, 2607, 2694
AccB7I CCANNNNNTGG 1 cut(s) 2433
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 2 cut(s) 927, 1814
AccII CGCG 1 cut(s) 2292
AciI CCGC 9 cut(s) 148, 226, 285, 1113, 1151, 1608, 1682, 1729, 1856
AclWI GGATC 8 cut(s) 1975, 1988, 2067, 2302, 2379, 2399, 2494, 2904
AcoI YGGCCR 4 cut(s) 1044, 1254, 1361, 1730
AcsI RAATTY 4 cut(s) 171, 328, 548, 707
AcuI CTGAAG 1 cut(s) 1745
AcvI CACGTG 1 cut(s) 1030
AcyI GRCGYC 1 cut(s) 916
AdeI CACNNNGTG 2 cut(s) 1042, 2549
AflII CTTAAG 2 cut(s) 233, 302
AflIII ACRYGT 2 cut(s) 2209, 2569
AgeI ACCGGT 1 cut(s) 3017
AgsI TTSAA 9 cut(s) 887, 1055, 1136, 1213, 1307, 1380, 1947, 2395, 2511
AjnI CCWGG 9 cut(s) 12, 930, 1046, 1999, 2151, 2426, 2479, 2610, 2970
AluBI AGCT 9 cut(s) 870, 891, 1209, 1345, 1853, 2104, 2371, 2465, 2647
AluI AGCT 9 cut(s) 870, 891, 1209, 1345, 1853, 2104, 2371, 2465, 2647
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 3 cut(s) 1447, 1825, 2081
AlwI GGATC 8 cut(s) 1975, 1988, 2067, 2302, 2379, 2399, 2494, 2904
AlwNI CAGNNNCTG 1 cut(s) 2053
Ama87I CYCGRG 2 cut(s) 2176, 2446
ApaI GGGCCC 1 cut(s) 274
ApeKI GCWGC 4 cut(s) 151, 196, 229, 2368
ApoI RAATTY 4 cut(s) 171, 328, 548, 707
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AseI ATTAAT 1 cut(s) 753
AsiGI ACCGGT 1 cut(s) 3017
Asp700I GAANNNNTTC 2 cut(s) 8, 1473
Asp718I GGTACC 1 cut(s) 2422
AspA2I CCTAGG 1 cut(s) 200
AspLEI GCGC 1 cut(s) 1705
AspS9I GGNCC 9 cut(s) 270, 271, 346, 828, 1079, 1228, 1314, 1997, 2995
AsuC2I CCSGG 2 cut(s) 2447, 2448
AsuHPI GGTGA 7 cut(s) 821, 854, 1180, 1471, 2414, 2732, 2797
AvaI CYCGRG 2 cut(s) 2176, 2446
AvaII GGWCC 3 cut(s) 1228, 1314, 2995
AvrII CCTAGG 1 cut(s) 200
AxyI CCTNAGG 3 cut(s) 275, 1016, 2272
BaeGI GKGCMC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 2414, 2447
BalI TGGCCA 2 cut(s) 1046, 1256
BamHI GGATCC 1 cut(s) 1980
BanI GGYRCC 5 cut(s) 419, 2333, 2422, 2607, 2694
BanII GRGCYC 2 cut(s) 274, 1855
BauI CACGAG 2 cut(s) 1265, 2410
BbrPI CACGTG 1 cut(s) 1030
BbsI GAAGAC 5 cut(s) 1015, 1511, 1837, 1888, 1947
Bbv12I GWGCWC 1 cut(s) 1855
BbvI GCAGC 4 cut(s) 138, 183, 216, 2380
BceAI ACGGC 4 cut(s) 77, 1232, 1348, 1850
BcgI CGANNNNNNTGC 2 cut(s) 2357, 2391
BciT130I CCWGG 9 cut(s) 14, 932, 1048, 2001, 2153, 2428, 2481, 2612, 2972
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 1 cut(s) 1218
BcnI CCSGG 2 cut(s) 2447, 2448
BcoDI GTCTC 3 cut(s) 1447, 1825, 2081
BfaI CTAG 7 cut(s) 201, 353, 390, 2204, 2225, 2766, 2861
BfmI CTRYAG 1 cut(s) 310
BfrI CTTAAG 2 cut(s) 233, 302
BfuAI ACCTGC 1 cut(s) 2390
BfuI GTATCC 1 cut(s) 725
BglI GCCNNNNNGGC 3 cut(s) 278, 1253, 1670
BisI GCNGC 8 cut(s) 149, 152, 197, 227, 230, 286, 1730, 2369
BlnI CCTAGG 1 cut(s) 200
BlsI GCNGC 8 cut(s) 150, 153, 198, 228, 231, 287, 1731, 2370
Bme18I GGWCC 3 cut(s) 1228, 1314, 2995
BmeT110I CYCGRG 2 cut(s) 2176, 2446
BmgT120I GGNCC 9 cut(s) 270, 271, 346, 828, 1079, 1228, 1314, 1997, 2995
BmsI GCATC 2 cut(s) 1914, 2697
BoxI GACNNNNGTC 1 cut(s) 2949
BpiI GAAGAC 5 cut(s) 1015, 1511, 1837, 1888, 1947
BplI GAGNNNNNCTC 6 cut(s) 1506, 1538, 2825, 2857, 2918, 2950
BpmI CTGGAG 1 cut(s) 2502
Bpu10I CCTNAGC 1 cut(s) 1699
BpuEI CTTGAG 4 cut(s) 764, 1507, 1807, 2057
BpuMI CCSGG 2 cut(s) 2447, 2448
Bsa29I ATCGAT 1 cut(s) 2062
BsaAI YACGTR 3 cut(s) 1030, 2570, 2799
BsaHI GRCGYC 1 cut(s) 916
BsaWI WCCGGW 3 cut(s) 1757, 1908, 3017
BsaXI ACNNNNNCTCC 4 cut(s) 1602, 1632, 2970, 3000
Bse118I RCCGGY 2 cut(s) 1732, 3017
Bse1I ACTGG 2 cut(s) 1461, 2017
Bse21I CCTNAGG 3 cut(s) 275, 1016, 2272
Bse3DI GCAATG 3 cut(s) 687, 1597, 2533
BseBI CCWGG 9 cut(s) 14, 932, 1048, 2001, 2153, 2428, 2481, 2612, 2972
BseCI ATCGAT 1 cut(s) 2062
BseGI GGATG 7 cut(s) 91, 197, 556, 1573, 1754, 1905, 2255
BseMI GCAATG 3 cut(s) 687, 1597, 2533
BseMII CTCAG 4 cut(s) 1030, 1654, 1810, 2286
BseNI ACTGG 2 cut(s) 1461, 2017
BseRI GAGGAG 2 cut(s) 2075, 2849
BseSI GKGCMC 1 cut(s) 274
BseX3I CGGCCG 1 cut(s) 1730
BseXI GCAGC 4 cut(s) 138, 183, 216, 2380
BseYI CCCAGC 1 cut(s) 2371
Bsh1236I CGCG 1 cut(s) 2292
Bsh1285I CGRYCG 1 cut(s) 1733
BshNI GGYRCC 5 cut(s) 419, 2333, 2422, 2607, 2694
BshTI ACCGGT 1 cut(s) 3017
BshVI ATCGAT 1 cut(s) 2062
BsiEI CGRYCG 1 cut(s) 1733
BsiHKAI GWGCWC 1 cut(s) 1855
BsiHKCI CYCGRG 2 cut(s) 2176, 2446
BsiSI CCGG 5 cut(s) 1733, 1758, 1909, 2447, 3018
BslFI GGGAC 2 cut(s) 2166, 2265
BsmAI GTCTC 3 cut(s) 1447, 1825, 2081
BsmFI GGGAC 2 cut(s) 2166, 2265
BsoBI CYCGRG 2 cut(s) 2176, 2446
Bsp120I GGGCCC 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 274, 1855
Bsp1407I TGTACA 1 cut(s) 1776
Bsp19I CCATGG 1 cut(s) 1917
Bsp68I TCGCGA 1 cut(s) 2292
BspACI CCGC 9 cut(s) 148, 226, 285, 1113, 1151, 1608, 1682, 1729, 1856
BspCNI CTCAG 4 cut(s) 1029, 1653, 1809, 2285
BspDI ATCGAT 1 cut(s) 2062
BspFNI CGCG 1 cut(s) 2292
BspHI TCATGA 1 cut(s) 495
BspMI ACCTGC 1 cut(s) 2390
BspPI GGATC 8 cut(s) 1975, 1988, 2067, 2302, 2379, 2399, 2494, 2904
BspT107I GGYRCC 5 cut(s) 419, 2333, 2422, 2607, 2694
BspTI CTTAAG 2 cut(s) 233, 302
BsrBI CCGCTC 1 cut(s) 148
BsrDI GCAATG 3 cut(s) 687, 1597, 2533
BsrFI RCCGGY 2 cut(s) 1732, 3017
BsrGI TGTACA 1 cut(s) 1776
BsrI ACTGG 2 cut(s) 1461, 2017
BssAI RCCGGY 2 cut(s) 1732, 3017
BssNI GRCGYC 1 cut(s) 916
BssSI CACGAG 2 cut(s) 1265, 2410
BssT1I CCWWGG 5 cut(s) 200, 1160, 1323, 1917, 2005
Bst2BI CACGAG 2 cut(s) 1265, 2410
Bst2UI CCWGG 9 cut(s) 14, 932, 1048, 2001, 2153, 2428, 2481, 2612, 2972
Bst4CI ACNGT 6 cut(s) 1232, 1650, 1819, 2141, 2288, 2301
Bst6I CTCTTC 2 cut(s) 1871, 2919
BstACI GRCGYC 1 cut(s) 916
BstAFI CTTAAG 2 cut(s) 233, 302
BstAPI GCANNNNNTGC 1 cut(s) 2549
BstAUI TGTACA 1 cut(s) 1776
BstBAI YACGTR 3 cut(s) 1030, 2570, 2799
BstC8I GCNNGC 5 cut(s) 283, 524, 1662, 1734, 2159
BstDSI CCRYGG 1 cut(s) 1917
BstEII GGTNACC 1 cut(s) 1270
BstF5I GGATG 7 cut(s) 91, 197, 556, 1573, 1754, 1905, 2255
BstFNI CGCG 1 cut(s) 2292
BstHHI GCGC 1 cut(s) 1705
BstMAI GTCTC 3 cut(s) 1447, 1825, 2081
BstMCI CGRYCG 1 cut(s) 1733
BstMWI GCNNNNNNNGC 8 cut(s) 278, 963, 1148, 1253, 1670, 1679, 1729, 2549
BstNI CCWGG 9 cut(s) 14, 932, 1048, 2001, 2153, 2428, 2481, 2612, 2972
BstNSI RCATGY 5 cut(s) 526, 762, 1286, 1372, 2213
BstPAI GACNNNNGTC 1 cut(s) 2949
BstPI GGTNACC 1 cut(s) 1270
BstSFI CTRYAG 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 1 cut(s) 2292
BstV1I GCAGC 4 cut(s) 138, 183, 216, 2380
BstV2I GAAGAC 5 cut(s) 1015, 1511, 1837, 1888, 1947
BstX2I RGATCY 4 cut(s) 1980, 2384, 2486, 2909
BstYI RGATCY 4 cut(s) 1980, 2384, 2486, 2909
BstZI CGGCCG 1 cut(s) 1730
Bsu15I ATCGAT 1 cut(s) 2062
Bsu36I CCTNAGG 3 cut(s) 275, 1016, 2272
BsuI GTATCC 1 cut(s) 725
BsuTUI ATCGAT 1 cut(s) 2062
BtgI CCRYGG 1 cut(s) 1917
BtgZI GCGATG 1 cut(s) 2468
BtsCI GGATG 7 cut(s) 91, 197, 556, 1573, 1754, 1905, 2255
BtsIMutI CAGTG 2 cut(s) 983, 1905
BtuMI TCGCGA 1 cut(s) 2292
BveI ACCTGC 1 cut(s) 2390
Cac8I GCNNGC 5 cut(s) 283, 524, 1662, 1734, 2159
CaiI CAGNNNCTG 1 cut(s) 2053
CciI TCATGA 1 cut(s) 495
CfoI GCGC 1 cut(s) 1705
Cfr10I RCCGGY 2 cut(s) 1732, 3017
Cfr13I GGNCC 9 cut(s) 270, 271, 346, 828, 1079, 1228, 1314, 1997, 2995
Cfr9I CCCGGG 1 cut(s) 2446
ClaI ATCGAT 1 cut(s) 2062
CseI GACGC 1 cut(s) 924
CspAI ACCGGT 1 cut(s) 3017
CspCI CAANNNNNGTGG 2 cut(s) 113, 148
DraIII CACNNNGTG 2 cut(s) 1042, 2549
DrdI GACNNNNNNGTC 1 cut(s) 2031
DseDI GACNNNNNNGTC 1 cut(s) 2031
EaeI YGGCCR 4 cut(s) 1044, 1254, 1361, 1730
EagI CGGCCG 1 cut(s) 1730
Eam1104I CTCTTC 2 cut(s) 1871, 2919
EarI CTCTTC 2 cut(s) 1871, 2919
EciI GGCGGA 2 cut(s) 1623, 1845
Ecl136II GAGCTC 1 cut(s) 1853
EclXI CGGCCG 1 cut(s) 1730
Eco130I CCWWGG 5 cut(s) 200, 1160, 1323, 1917, 2005
Eco147I AGGCCT 1 cut(s) 1660
Eco24I GRGCYC 2 cut(s) 274, 1855
Eco47I GGWCC 3 cut(s) 1228, 1314, 2995
Eco52I CGGCCG 1 cut(s) 1730
Eco53kI GAGCTC 1 cut(s) 1853
Eco57I CTGAAG 1 cut(s) 1745
Eco72I CACGTG 1 cut(s) 1030
Eco81I CCTNAGG 3 cut(s) 275, 1016, 2272
Eco88I CYCGRG 2 cut(s) 2176, 2446
Eco91I GGTNACC 1 cut(s) 1270
EcoICRI GAGCTC 1 cut(s) 1853
EcoO109I RGGNCCY 3 cut(s) 270, 271, 2995
EcoO65I GGTNACC 1 cut(s) 1270
EcoRII CCWGG 9 cut(s) 12, 930, 1046, 1999, 2151, 2426, 2479, 2610, 2970
EcoT14I CCWWGG 5 cut(s) 200, 1160, 1323, 1917, 2005
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 2 cut(s) 274, 1855
ErhI CCWWGG 5 cut(s) 200, 1160, 1323, 1917, 2005
FalI AAGNNNNNCTT 2 cut(s) 2019, 2051
FaqI GGGAC 2 cut(s) 2166, 2265
FauNDI CATATG 2 cut(s) 723, 2948
FbaI TGATCA 1 cut(s) 1218
FblI GTMKAC 2 cut(s) 927, 1814
Fnu4HI GCNGC 8 cut(s) 149, 152, 197, 227, 230, 286, 1730, 2369
FokI GGATG 7 cut(s) 98, 204, 543, 1580, 1741, 1892, 2262
FriOI GRGCYC 2 cut(s) 274, 1855
Fsp4HI GCNGC 8 cut(s) 149, 152, 197, 227, 230, 286, 1730, 2369
FspBI CTAG 7 cut(s) 201, 353, 390, 2204, 2225, 2766, 2861
GlaI GCGC 1 cut(s) 1704
GluI GCNGC 8 cut(s) 149, 152, 197, 227, 230, 286, 1730, 2369
GsaI CCCAGC 1 cut(s) 2375
GsuI CTGGAG 1 cut(s) 2502
HapII CCGG 5 cut(s) 1733, 1758, 1909, 2447, 3018
HgaI GACGC 1 cut(s) 924
HhaI GCGC 1 cut(s) 1705
Hin1I GRCGYC 1 cut(s) 916
Hin6I GCGC 1 cut(s) 1703
HinP1I GCGC 1 cut(s) 1703
HincII GTYRAC 4 cut(s) 790, 928, 2439, 3015
HindII GTYRAC 4 cut(s) 790, 928, 2439, 3015
HindIII AAGCTT 3 cut(s) 889, 1207, 2102
HinfI GANTC 9 cut(s) 542, 935, 1092, 1751, 1811, 1925, 2044, 2179, 2681
HpaII CCGG 5 cut(s) 1733, 1758, 1909, 2447, 3018
HphI GGTGA 7 cut(s) 821, 854, 1180, 1471, 2414, 2732, 2797
Hpy188I TCNGA 6 cut(s) 513, 679, 1097, 1643, 1850, 2938
Hpy188III TCNNGA 9 cut(s) 353, 390, 496, 2036, 2261, 2274, 2282, 2291, 2378
Hpy99I CGWCG 2 cut(s) 918, 2267
HpyCH4III ACNGT 6 cut(s) 1232, 1650, 1819, 2141, 2288, 2301
HpyCH4IV ACGT 6 cut(s) 593, 1029, 2265, 2569, 2798, 2820
HpyCH4V TGCA 9 cut(s) 474, 648, 758, 1368, 1905, 2326, 2543, 2552, 2710
HpyF10VI GCNNNNNNNGC 8 cut(s) 278, 963, 1148, 1253, 1670, 1679, 1729, 2549
HpySE526I ACGT 6 cut(s) 593, 1029, 2265, 2569, 2798, 2820
Hsp92I GRCGYC 1 cut(s) 916
HspAI GCGC 1 cut(s) 1703
KpnI GGTACC 1 cut(s) 2426
KroI GCCGGC 1 cut(s) 1732
KroNI GCCGGC 1 cut(s) 1734
Ksp22I TGATCA 1 cut(s) 1218
LmnI GCTCC 6 cut(s) 145, 572, 663, 1858, 2482, 2584
Lsp1109I GCAGC 4 cut(s) 138, 183, 216, 2380
LweI GCATC 2 cut(s) 1914, 2697
MaeI CTAG 7 cut(s) 201, 353, 390, 2204, 2225, 2766, 2861
MaeII ACGT 6 cut(s) 593, 1029, 2265, 2569, 2798, 2820
MaeIII GTNAC 3 cut(s) 682, 1270, 1748
MbiI CCGCTC 1 cut(s) 148
MfeI CAATTG 2 cut(s) 1136, 2234
MflI RGATCY 4 cut(s) 1980, 2384, 2486, 2909
MhlI GDGCHC 2 cut(s) 274, 1855
MlsI TGGCCA 2 cut(s) 1046, 1256
MluNI TGGCCA 2 cut(s) 1046, 1256
MlyI GAGTC 6 cut(s) 536, 1745, 1820, 1919, 2053, 2188
MmeI TCCRAC 8 cut(s) 82, 683, 1066, 1512, 2578, 2638, 2881, 2956
Mox20I TGGCCA 2 cut(s) 1046, 1256
Mph1103I ATGCAT 1 cut(s) 760
MroNI GCCGGC 1 cut(s) 1732
MroXI GAANNNNTTC 2 cut(s) 8, 1473
MscI TGGCCA 2 cut(s) 1046, 1256
MslI CAYNNNNRTG 3 cut(s) 653, 726, 1287
Msp20I TGGCCA 2 cut(s) 1046, 1256
MspA1I CMGCKG 1 cut(s) 2371
MspCI CTTAAG 2 cut(s) 233, 302
MspI CCGG 5 cut(s) 1733, 1758, 1909, 2447, 3018
MunI CAATTG 2 cut(s) 1136, 2234
MvaI CCWGG 9 cut(s) 14, 932, 1048, 2001, 2153, 2428, 2481, 2612, 2972
MvnI CGCG 1 cut(s) 2292
MwoI GCNNNNNNNGC 8 cut(s) 278, 963, 1148, 1253, 1670, 1679, 1729, 2549
NaeI GCCGGC 1 cut(s) 1734
NciI CCSGG 2 cut(s) 2447, 2448
NcoI CCATGG 1 cut(s) 1917
NdeI CATATG 2 cut(s) 723, 2948
NgoMIV GCCGGC 1 cut(s) 1732
NmeAIII GCCGAG 3 cut(s) 100, 1298, 1584
NmuCI GTSAC 1 cut(s) 1748
NruI TCGCGA 1 cut(s) 2292
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 5 cut(s) 526, 762, 1286, 1372, 2213
PaeI GCATGC 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 2176
PagI TCATGA 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 2390
PceI AGGCCT 1 cut(s) 1660
PciI ACATGT 1 cut(s) 2209
PcsI WCGNNNNNNNCGW 2 cut(s) 590, 2183
PdiI GCCGGC 1 cut(s) 1734
PdmI GAANNNNTTC 2 cut(s) 8, 1473
PfeI GAWTC 3 cut(s) 935, 1092, 2681
PflMI CCANNNNNTGG 1 cut(s) 2433
PfoI TCCNGGA 1 cut(s) 2479
PinAI ACCGGT 1 cut(s) 3017
PkrI GCNGC 8 cut(s) 150, 153, 198, 228, 231, 287, 1731, 2370
PleI GAGTC 6 cut(s) 536, 1745, 1819, 1919, 2052, 2187
PmaCI CACGTG 1 cut(s) 1030
PmlI CACGTG 1 cut(s) 1030
PpsI GAGTC 6 cut(s) 536, 1745, 1819, 1919, 2052, 2187
Ppu21I YACGTR 3 cut(s) 1030, 2570, 2799
PpuMI RGGWCCY 1 cut(s) 2995
PscI ACATGT 1 cut(s) 2209
PshAI GACNNNNGTC 1 cut(s) 2949
PshBI ATTAAT 1 cut(s) 753
PsiI TTATAA 1 cut(s) 1622
Psp124BI GAGCTC 1 cut(s) 1855
Psp5II RGGWCCY 1 cut(s) 2995
Psp6I CCWGG 9 cut(s) 12, 930, 1046, 1999, 2151, 2426, 2479, 2610, 2970
PspCI CACGTG 1 cut(s) 1030
PspEI GGTNACC 1 cut(s) 1270
PspFI CCCAGC 1 cut(s) 2371
PspGI CCWGG 9 cut(s) 12, 930, 1046, 1999, 2151, 2426, 2479, 2610, 2970
PspOMI GGGCCC 1 cut(s) 270
PspPI GGNCC 9 cut(s) 270, 271, 346, 828, 1079, 1228, 1314, 1997, 2995
PspPPI RGGWCCY 1 cut(s) 2995
PspXI VCTCGAGB 1 cut(s) 2176
PstNI CAGNNNCTG 1 cut(s) 2053
PsuI RGATCY 4 cut(s) 1980, 2384, 2486, 2909
PvuII CAGCTG 1 cut(s) 2371
RruI TCGCGA 1 cut(s) 2292
RseI CAYNNNNRTG 3 cut(s) 653, 726, 1287
SacI GAGCTC 1 cut(s) 1855
SalI GTCGAC 1 cut(s) 926
SatI GCNGC 8 cut(s) 149, 152, 197, 227, 230, 286, 1730, 2369
Sau96I GGNCC 9 cut(s) 270, 271, 346, 828, 1079, 1228, 1314, 1997, 2995
SchI GAGTC 6 cut(s) 536, 1745, 1820, 1919, 2053, 2188
SduI GDGCHC 2 cut(s) 274, 1855
SfaNI GCATC 2 cut(s) 1914, 2697
SfcI CTRYAG 1 cut(s) 310
Sfr274I CTCGAG 1 cut(s) 2176
SinI GGWCC 3 cut(s) 1228, 1314, 2995
SlaI CTCGAG 1 cut(s) 2176
SmaI CCCGGG 1 cut(s) 2448
SmiMI CAYNNNNRTG 3 cut(s) 653, 726, 1287
SmlI CTYRAG 7 cut(s) 233, 302, 743, 1486, 1822, 2036, 2176
SmoI CTYRAG 7 cut(s) 233, 302, 743, 1486, 1822, 2036, 2176
SphI GCATGC 1 cut(s) 526
SseBI AGGCCT 1 cut(s) 1660
SsiI CCGC 9 cut(s) 148, 226, 285, 1113, 1151, 1608, 1682, 1729, 1856
SspMI CTAG 7 cut(s) 201, 353, 390, 2204, 2225, 2766, 2861
SstI GAGCTC 1 cut(s) 1855
StuI AGGCCT 1 cut(s) 1660
StyI CCWWGG 5 cut(s) 200, 1160, 1323, 1917, 2005
TaaI ACNGT 6 cut(s) 1232, 1650, 1819, 2141, 2288, 2301
TaiI ACGT 6 cut(s) 596, 1032, 2268, 2572, 2801, 2823
TaqI TCGA 8 cut(s) 927, 1976, 2062, 2166, 2177, 2262, 2377, 2616
TaqII GACCGA 1 cut(s) 927
TatI WGTACW 2 cut(s) 1776, 2875
TauI GCSGC 4 cut(s) 151, 229, 288, 1732
TfiI GAWTC 3 cut(s) 935, 1092, 2681
TscAI CASTG 2 cut(s) 983, 1905
TseFI GTSAC 1 cut(s) 1748
TseI GCWGC 4 cut(s) 151, 196, 229, 2368
Tsp45I GTSAC 1 cut(s) 1748
TspGWI ACGGA 2 cut(s) 1238, 2059
TspMI CCCGGG 1 cut(s) 2446
TspRI CASTG 2 cut(s) 983, 1905
Van91I CCANNNNNTGG 1 cut(s) 2433
Vha464I CTTAAG 2 cut(s) 233, 302
VpaK11BI GGWCC 3 cut(s) 1228, 1314, 2995
VspI ATTAAT 1 cut(s) 753
XapI RAATTY 4 cut(s) 171, 328, 548, 707
XbaI TCTAGA 2 cut(s) 352, 389
XceI RCATGY 5 cut(s) 526, 762, 1286, 1372, 2213
XcmI CCANNNNNNNNNTGG 3 cut(s) 803, 1264, 2007
XhoI CTCGAG 1 cut(s) 2176
XmaI CCCGGG 1 cut(s) 2446
XmaJI CCTAGG 1 cut(s) 200
XmiI GTMKAC 2 cut(s) 927, 1814
XmnI GAANNNNTTC 2 cut(s) 8, 1473
XspI CTAG 7 cut(s) 201, 353, 390, 2204, 2225, 2766, 2861
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.