pycom12g09610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016957
Physical Location & Seq
Forward (+)
11494544 .. 11495200
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g09610.1

Sequence Viewer

Length: 534 bp
ATGATGAACAGCGATGATAGGGTCCAGGCCGGGCATTTCTTTGTAGGTCCAAGCAAAGACGTCTTTAATGCACTTACGAAGATAGGCTTTGGTTCCTTGCTTGTACCTAAGTTGAGTTCACTGATCGATCATCGACTGTGGCTTGCCCCCCATCTTCGAGCTGTGGTGACCTCCTCTTCAGTGCTATCTTCTCGAGCTTGTTGGCATAAGGATTGTCTGGTGTGGATGATAGTACGCCTTCTTACTTTCAGTGGTCCCTTTGTGTCGACCTCTAGGATTGCTTGGCGCTTCATCCTTGAAGGAATTAAGCTTCGAACATCGCCTTTTTCTGCTAACCCATTGAGCTTTTCTTCCTTTGATGTTGTCTTCCTCTTTCCAAAAGGTTTCTCATTATCTTCAAATCTTTCCGGAGCTGATCGTCGCAGAGGAGAGATAGCTGTGTTGCTTTGTTTCTTAGGCTTTAACAACCTTTCAAAAACAGAGCTTTGGGATATTGGCGCGTTAAGCCTCTTGAAGACAGAGGTTCGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.7

Weight (kDa)

10.03

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 266
AccII CGCG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 2 cut(s) 105, 234
AgsI TTSAA 4 cut(s) 299, 399, 474, 514
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 7 cut(s) 161, 197, 310, 345, 413, 437, 484
AluI AGCT 7 cut(s) 161, 197, 310, 345, 413, 437, 484
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 407
AoxI GGCC 1 cut(s) 27
AspLEI GCGC 2 cut(s) 288, 500
AspS9I GGNCC 3 cut(s) 22, 47, 254
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 178
AsuII TTCGAA 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 3 cut(s) 22, 47, 254
BbsI GAAGAC 2 cut(s) 358, 521
BccI CCATC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 26
BcnI CCSGG 1 cut(s) 31
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 289
Bme1390I CCNGG 2 cut(s) 26, 31
Bme18I GGWCC 3 cut(s) 22, 47, 254
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 22, 47, 254
BmiI GGNNCC 3 cut(s) 23, 94, 256
BmrFI CCNGG 2 cut(s) 26, 31
BpiI GAAGAC 2 cut(s) 358, 521
Bpu14I TTCGAA 1 cut(s) 313
BpuMI CCSGG 1 cut(s) 31
Bsa29I ATCGAT 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 407
BseAI TCCGGA 1 cut(s) 407
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 126
BseGI GGATG 2 cut(s) 231, 291
BseRI GAGGAG 2 cut(s) 163, 441
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 1 cut(s) 29
BshVI ATCGAT 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 2 cut(s) 30, 408
BslFI GGGAC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 240
BsnI GGCC 1 cut(s) 29
BsoBI CYCGRG 1 cut(s) 192
Bsp119I TTCGAA 1 cut(s) 313
Bsp13I TCCGGA 1 cut(s) 407
Bsp143I GATC 3 cut(s) 123, 127, 415
BspANI GGCC 1 cut(s) 29
BspDI ATCGAT 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 407
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 3 cut(s) 23, 94, 256
BspT104I TTCGAA 1 cut(s) 313
BssMI GATC 3 cut(s) 123, 127, 415
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 313
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 108, 454
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 2 cut(s) 231, 291
BstFNI CGCG 1 cut(s) 500
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 2 cut(s) 288, 500
BstKTI GATC 3 cut(s) 126, 130, 418
BstMBI GATC 3 cut(s) 123, 127, 415
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstNI CCWGG 1 cut(s) 26
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 24, 29
BstUI CGCG 1 cut(s) 500
BstV2I GAAGAC 2 cut(s) 358, 521
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 126
BtgZI GCGATG 2 cut(s) 27, 303
BtsCI GGATG 2 cut(s) 231, 291
BtsIMutI CAGTG 3 cut(s) 119, 186, 256
Cac8I GCNNGC 1 cut(s) 144
CfoI GCGC 2 cut(s) 288, 500
Cfr13I GGNCC 3 cut(s) 22, 47, 254
ClaI ATCGAT 1 cut(s) 126
Csp6I GTAC 2 cut(s) 104, 233
CviQI GTAC 2 cut(s) 104, 233
DdeI CTNAG 2 cut(s) 108, 454
DpnI GATC 3 cut(s) 125, 129, 417
DpnII GATC 3 cut(s) 123, 127, 415
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
Eco47I GGWCC 3 cut(s) 22, 47, 254
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 192
Eco91I GGTNACC 1 cut(s) 166
EcoO65I GGTNACC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 24
FaiI YATR 1 cut(s) 207
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 1 cut(s) 240
FblI GTMKAC 1 cut(s) 266
FokI GGATG 2 cut(s) 238, 278
FspBI CTAG 1 cut(s) 273
GlaI GCGC 2 cut(s) 287, 499
HaeII RGCGCY 1 cut(s) 289
HaeIII GGCC 1 cut(s) 29
HapII CCGG 2 cut(s) 30, 408
HhaI GCGC 2 cut(s) 288, 500
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 2 cut(s) 286, 498
HinP1I GCGC 2 cut(s) 286, 498
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 308
HpaII CCGG 2 cut(s) 30, 408
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 119, 267
Hpy188III TCNNGA 4 cut(s) 192, 408, 511, 531
Hpy8I GTNNAC 2 cut(s) 119, 267
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 2 cut(s) 248, 293
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 2 cut(s) 108, 454
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 2 cut(s) 286, 498
Kpn2I TCCGGA 1 cut(s) 407
Kzo9I GATC 3 cut(s) 123, 127, 415
LmnI GCTCC 1 cut(s) 410
LpnPI CCDG 5 cut(s) 11, 38, 43, 203, 421
MaeI CTAG 1 cut(s) 273
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 125, 129, 417
MboI GATC 3 cut(s) 123, 127, 415
MboII GAAGA 8 cut(s) 91, 146, 168, 180, 342, 358, 387, 526
MluCI AATT 1 cut(s) 303
MnlI CCTC 7 cut(s) 181, 184, 280, 380, 419, 514, 518
MroI TCCGGA 1 cut(s) 407
MseI TTAA 4 cut(s) 66, 306, 462, 503
MspI CCGG 2 cut(s) 30, 408
MspR9I CCNGG 2 cut(s) 26, 31
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 1 cut(s) 504
NciI CCSGG 1 cut(s) 31
NdeII GATC 3 cut(s) 123, 127, 415
NlaIV GGNNCC 3 cut(s) 23, 94, 256
NmuCI GTSAC 1 cut(s) 166
NspV TTCGAA 1 cut(s) 313
PaeR7I CTCGAG 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 24
PspEI GGTNACC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 23, 94, 256
PspPI GGNCC 3 cut(s) 22, 47, 254
RsaI GTAC 2 cut(s) 105, 234
RsaNI GTAC 2 cut(s) 104, 233
SalI GTCGAC 1 cut(s) 265
SaqAI TTAA 4 cut(s) 66, 306, 462, 503
Sau3AI GATC 3 cut(s) 123, 127, 415
Sau96I GGNCC 3 cut(s) 22, 47, 254
ScrFI CCNGG 2 cut(s) 26, 31
Sfr274I CTCGAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 313
SinI GGWCC 3 cut(s) 22, 47, 254
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 1 cut(s) 303
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 24, 29
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 63
TaqI TCGA 6 cut(s) 126, 133, 157, 193, 266, 313
TaqII GACCGA 1 cut(s) 516
TasI AATT 1 cut(s) 303
Tru1I TTAA 4 cut(s) 66, 306, 462, 503
Tru9I TTAA 4 cut(s) 66, 306, 462, 503
TscAI CASTG 3 cut(s) 126, 186, 256
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 20, 280
TspRI CASTG 3 cut(s) 126, 186, 256
VpaK11BI GGWCC 3 cut(s) 22, 47, 254
XhoI CTCGAG 1 cut(s) 192
XmiI GTMKAC 1 cut(s) 266
XspI CTAG 1 cut(s) 273
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.