pycom13g23310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016957
Physical Location & Seq
Forward (+)
20925608 .. 20926822
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23310.5

Sequence Viewer

Length: 879 bp
ATGATCTCGTGTTGGTCGAGAAGCAACGCCCATTTAGCTAGTCGCCCTGTCAAAACCATATTGGACATAATGTACTTGACCGAGTCGGCTTTCGTAACCAAGTGGATGGTACTAGGGCAAGGCACATCTTCTCAATTGGTGAGTATGCTATTTCCTTTTGCTCTTCATTTTCTTATGCTAAAAGTGCTCCAACTGAACTTTCTTGTGTGGCAATACAGGTGGGCTTTCCAGGTACCGTTTTATGCTCTCGAAGGCATTGCGGTAGGCATTATCCCATATGAATGGAGCGTCCTTCTTCATGAGTCGACTGAAAGTTTGACAACGTCTAGTGACGTTGGAGATGAAGTGTCTAATGATGGCAAGCTGCTAGTAGCCTTGATCTTTGATTGGCCTACTTCAATGTCATGGTGCTTGAAAACAAAGCTGAGGAACTTCCCAGAGGTGACTGTCGTAGTTTGTCGAACAGCATTCGTAAATCCTTCAAGTGATCGATCTCTTTTTGGTCTTGACAACTACGTCGTCTATGTAACATTCTACGTTTTTGTAGCCATCATTGAAGATTTTTTGCATCGTGCGTTGATATGTATAGATACCTTTTGGAGTACGGAAGTGTATCCAGAGGAGTCGTCCATGAATAACAGTGCCTCGTGGCCATTGGTTGCGTCAACCATGATTTTGATGATTGGCAAGGGAAAGTCATCCTTCGGACAAGCATCGTTGAGGTCGAAGAAATCTACGCAAACACATATTTTTCTAGATTTCTTAAGGACAATGACAATGTTGGAGATCCATTTAGGGTATTGCACCTCTCGAATGAAGCTTGCTTTGATTAGCTTGTCAATCTCAGTCTCAATTTGCGGGATGAGCTTAGATCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.08

Weight (kDa)

6.59

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 232
AccB1I GGYRCC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 305
AciI CCGC 2 cut(s) 260, 858
AclWI GGATC 1 cut(s) 781
AcoI YGGCCR 1 cut(s) 650
AfaI GTAC 4 cut(s) 74, 111, 234, 604
AflII CTTAAG 1 cut(s) 763
AgsI TTSAA 4 cut(s) 399, 415, 483, 557
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 6 cut(s) 38, 364, 424, 820, 834, 867
AluI AGCT 6 cut(s) 38, 364, 424, 820, 834, 867
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 853
AlwI GGATC 1 cut(s) 781
AoxI GGCC 2 cut(s) 389, 650
ApeKI GCWGC 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 298, 330
Asp718I GGTACC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 151, 454
BalI TGGCCA 1 cut(s) 652
BanI GGYRCC 1 cut(s) 232
BauI CACGAG 2 cut(s) 7, 646
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 351
BccI CCATC 3 cut(s) 100, 350, 557
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 230
BciVI GTATCC 1 cut(s) 624
BcoDI GTCTC 1 cut(s) 853
BfaI CTAG 5 cut(s) 39, 113, 327, 368, 755
BfrI CTTAAG 1 cut(s) 763
BfuI GTATCC 1 cut(s) 624
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 577, 722
Bpu10I CCTNAGC 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 230
BseCI ATCGAT 1 cut(s) 490
BseGI GGATG 3 cut(s) 111, 698, 867
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 416, 858
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 1 cut(s) 351
BshFI GGCC 2 cut(s) 391, 652
BshNI GGYRCC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 853
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 2 cut(s) 391, 652
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 6 cut(s) 3, 378, 487, 491, 786, 871
BspACI CCGC 2 cut(s) 260, 858
BspANI GGCC 2 cut(s) 391, 652
BspCNI CTCAG 2 cut(s) 417, 857
BspDI ATCGAT 1 cut(s) 490
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 781
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 232
BspTI CTTAAG 1 cut(s) 763
BsrDI GCAATG 1 cut(s) 255
BssMI GATC 6 cut(s) 3, 378, 487, 491, 786, 871
BssSI CACGAG 2 cut(s) 7, 646
Bst2BI CACGAG 2 cut(s) 7, 646
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 3 cut(s) 237, 448, 641
Bst6I CTCTTC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 763
BstC8I GCNNGC 2 cut(s) 362, 822
BstDEI CTNAG 3 cut(s) 425, 844, 868
BstF5I GGATG 3 cut(s) 111, 698, 867
BstKTI GATC 6 cut(s) 6, 381, 490, 494, 789, 874
BstMAI GTCTC 1 cut(s) 853
BstMBI GATC 6 cut(s) 3, 378, 487, 491, 786, 871
BstMWI GCNNNNNNNGC 3 cut(s) 35, 184, 864
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 786
BstXI CCANNNNNNTGG 2 cut(s) 106, 282
BstYI RGATCY 1 cut(s) 786
Bsu15I ATCGAT 1 cut(s) 490
BsuI GTATCC 1 cut(s) 624
BsuRI GGCC 2 cut(s) 391, 652
BsuTUI ATCGAT 1 cut(s) 490
BtsCI GGATG 3 cut(s) 111, 698, 867
BtsIMutI CAGTG 1 cut(s) 646
Cac8I GCNNGC 2 cut(s) 362, 822
CciI TCATGA 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 490
CseI GACGC 2 cut(s) 277, 651
Csp6I GTAC 4 cut(s) 73, 110, 233, 603
CspCI CAANNNNNGTGG 2 cut(s) 200, 235
CviAII CATG 4 cut(s) 299, 405, 631, 670
CviQI GTAC 4 cut(s) 73, 110, 233, 603
DdeI CTNAG 3 cut(s) 425, 844, 868
DpnI GATC 6 cut(s) 5, 380, 489, 493, 788, 873
DpnII GATC 6 cut(s) 3, 378, 487, 491, 786, 871
DrdI GACNNNNNNGTC 1 cut(s) 515
DseDI GACNNNNNNGTC 1 cut(s) 515
EaeI YGGCCR 1 cut(s) 650
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 228
FaeI CATG 4 cut(s) 302, 408, 634, 673
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FatI CATG 4 cut(s) 298, 404, 630, 669
FauI CCCGC 1 cut(s) 851
FauNDI CATATG 1 cut(s) 277
FblI GTMKAC 1 cut(s) 305
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 3 cut(s) 118, 685, 874
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 5 cut(s) 39, 113, 327, 368, 755
GluI GCNGC 1 cut(s) 365
HaeIII GGCC 2 cut(s) 391, 652
HgaI GACGC 2 cut(s) 277, 651
Hin1II CATG 4 cut(s) 302, 408, 634, 673
HincII GTYRAC 2 cut(s) 306, 666
HindII GTYRAC 2 cut(s) 306, 666
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 83, 302, 623
HphI GGTGA 2 cut(s) 151, 454
Hpy166II GTNNAC 2 cut(s) 306, 666
Hpy188I TCNGA 1 cut(s) 707
Hpy188III TCNNGA 7 cut(s) 18, 248, 299, 506, 617, 755, 810
Hpy8I GTNNAC 2 cut(s) 306, 666
Hpy99I CGWCG 1 cut(s) 521
HpyAV CCTTC 4 cut(s) 245, 302, 489, 712
HpyCH4III ACNGT 3 cut(s) 237, 448, 641
HpyCH4IV ACGT 4 cut(s) 323, 333, 516, 537
HpyCH4V TGCA 2 cut(s) 568, 804
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 184, 864
HpyF3I CTNAG 3 cut(s) 425, 844, 868
HpySE526I ACGT 4 cut(s) 323, 333, 516, 537
Hsp92II CATG 4 cut(s) 302, 408, 634, 673
KpnI GGTACC 1 cut(s) 236
Kzo9I GATC 6 cut(s) 3, 378, 487, 491, 786, 871
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 2 cut(s) 192, 285
LpnPI CCDG 6 cut(s) 60, 202, 215, 242, 450, 630
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 2 cut(s) 577, 722
MaeI CTAG 5 cut(s) 39, 113, 327, 368, 755
MaeII ACGT 4 cut(s) 323, 333, 516, 537
MaeIII GTNAC 4 cut(s) 94, 329, 442, 526
MalI GATC 6 cut(s) 5, 380, 489, 493, 788, 873
MboI GATC 6 cut(s) 3, 378, 487, 491, 786, 871
MboII GAAGA 5 cut(s) 120, 155, 287, 569, 739
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 1 cut(s) 786
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 652
MluCI AATT 2 cut(s) 134, 852
MluNI TGGCCA 1 cut(s) 652
MlyI GAGTC 3 cut(s) 92, 311, 632
MmeI TCCRAC 3 cut(s) 214, 316, 762
MnlI CCTC 6 cut(s) 420, 433, 613, 655, 714, 817
Mox20I TGGCCA 1 cut(s) 652
MscI TGGCCA 1 cut(s) 652
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 1 cut(s) 280
Msp20I TGGCCA 1 cut(s) 652
MspCI CTTAAG 1 cut(s) 763
MspR9I CCNGG 1 cut(s) 230
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 35, 184, 864
NdeI CATATG 1 cut(s) 277
NdeII GATC 6 cut(s) 3, 378, 487, 491, 786, 871
NlaIII CATG 4 cut(s) 302, 408, 634, 673
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 2 cut(s) 329, 442
PagI TCATGA 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 722
PctI GAATGC 1 cut(s) 467
PflFI GACNNNGTC 2 cut(s) 82, 322
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 3 cut(s) 91, 310, 631
PpsI GAGTC 3 cut(s) 91, 310, 631
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 786
PsyI GACNNNGTC 2 cut(s) 82, 322
RsaI GTAC 4 cut(s) 74, 111, 234, 604
RsaNI GTAC 4 cut(s) 73, 110, 233, 603
RseI CAYNNNNRTG 1 cut(s) 280
SalI GTCGAC 1 cut(s) 304
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 6 cut(s) 3, 378, 487, 491, 786, 871
SchI GAGTC 3 cut(s) 92, 311, 632
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 2 cut(s) 577, 722
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 763
SmoI CTYRAG 1 cut(s) 763
Sse9I AATT 2 cut(s) 134, 852
SsiI CCGC 2 cut(s) 260, 858
SspMI CTAG 5 cut(s) 39, 113, 327, 368, 755
StyD4I CCNGG 1 cut(s) 228
TaaI ACNGT 3 cut(s) 237, 448, 641
TaiI ACGT 4 cut(s) 326, 336, 519, 540
TaqI TCGA 7 cut(s) 17, 249, 305, 460, 490, 725, 811
TaqII GACCGA 1 cut(s) 95
TasI AATT 2 cut(s) 134, 852
TatI WGTACW 1 cut(s) 72
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 2 cut(s) 329, 442
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 2 cut(s) 329, 442
TspDTI ATGAA 6 cut(s) 155, 287, 294, 357, 647, 830
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 1 cut(s) 646
Tth111I GACNNNGTC 2 cut(s) 82, 322
Vha464I CTTAAG 1 cut(s) 763
XbaI TCTAGA 1 cut(s) 754
XmiI GTMKAC 1 cut(s) 305
XspI CTAG 5 cut(s) 39, 113, 327, 368, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.