pycom05g13600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013073
Physical Location & Seq
Reverse (-)
17138055 .. 17138867
813 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13600.2

Sequence Viewer

Length: 666 bp
ATGATAAACTCCAAGAAGAATTTGAAATTTAACAAAAACTCCATGGACCCCACCACCCCCACCGTGGCCCCACACATCACGAATAACAACAGCCTGTATGGAGCTGTGAACGCTACATTTCACCACCCCACCTCCTCAAAAACACAAAAACAAAACCCCCCACGAACCACATTTCCCCATCCTCCTCTGTTCTCCTCTGTTTTTCTCCATTTTCTCGGAGCTCGAATTCAGAGAAAAATGCCCGCAAATCATTCACTTCCAGTTTCTCAGGAGCAATTAACAGAGCAGCCGGGGCTGCGCAGACGGCTGTCGTCTCTGTCCCTCAAGCTCCAGCCCATCTCCTCCCCTGCAACTTCATGGGCACTAAGACGGTCGAAGTCGGTTTCCGCCATGGGAGAGTACGCCGGAGGATCCATAAGAAAATGGTGGGTCCAGAGCTGGGCATGGATCATGTCCAGAAAACCCATTTTTGCCCAGGATCTGGAGATGAACGAGGAAGAAACCAAAGTGCTCGGATCCGACAACAGAGGAAGCTTGAGGCATGTGTTCTACAACGTCCGGTCGGAGCTCAAGAAACTCATGGGTTCTGATGTCGGAAACCTGCCGCAAACTTACAGGTACGATTTTGATGATGGGAGGACCAAAACCAAGAAAGTTTACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.12

Weight (kDa)

10.33

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17300 AT4G35320
fragaria_vesca FvH4_2g16910
malus_domestica MD05G1140900.v1.1 MD11G1242200.v1.1
pyrus_communis pycom05g13600 pycom10g12220
rosa_chinensis RchiOBHm_Chr6g0281051
rosa_laevigata RLG00000013001
rosa_multiflora Rmu_sc0012869.1_g000008
rosa_roxburghii Rroxscaffold_7G00187350
rosa_rugosa Rorug06G0093600 Rorug06G0138300
rosa_samantha Rh6BG253400 Rh6CG253300 Rh6DG244900
rosa_wichuraiana Rw6G021740 Rw7G017000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 299
Acc36I ACCTGC 1 cut(s) 609
AccB7I CCANNNNNTGG 1 cut(s) 481
AciI CCGC 3 cut(s) 243, 387, 605
AclWI GGATC 6 cut(s) 405, 418, 455, 486, 510, 523
AcsI RAATTY 3 cut(s) 19, 26, 225
AfaI GTAC 2 cut(s) 401, 620
AfiI CCNNNNNNNGG 3 cut(s) 64, 439, 481
AgsI TTSAA 1 cut(s) 25
AjnI CCWGG 1 cut(s) 474
AloI GAACNNNNNNTCC 2 cut(s) 157, 189
AluBI AGCT 6 cut(s) 104, 221, 328, 438, 534, 568
AluI AGCT 6 cut(s) 104, 221, 328, 438, 534, 568
Alw21I GWGCWC 3 cut(s) 223, 513, 570
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 6 cut(s) 405, 418, 455, 486, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 481
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 286, 295
ApoI RAATTY 3 cut(s) 19, 26, 225
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 4 cut(s) 46, 67, 430, 639
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 113
AvaII GGWCC 3 cut(s) 46, 430, 639
BaeGI GKGCMC 1 cut(s) 364
BamHI GGATCC 2 cut(s) 410, 515
BanII GRGCYC 2 cut(s) 223, 570
Bbv12I GWGCWC 3 cut(s) 223, 513, 570
BbvI GCAGC 2 cut(s) 282, 298
BccI CCATC 3 cut(s) 186, 344, 626
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 476
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 609
BisI GCNGC 3 cut(s) 287, 296, 605
BlsI GCNGC 3 cut(s) 288, 297, 606
Bme1390I CCNGG 2 cut(s) 291, 476
Bme18I GGWCC 3 cut(s) 46, 430, 639
BmgT120I GGNCC 4 cut(s) 46, 67, 430, 639
BmiI GGNNCC 5 cut(s) 48, 69, 412, 431, 517
BmrFI CCNGG 2 cut(s) 291, 476
BoxI GACNNNNGTC 1 cut(s) 307
BpmI CTGGAG 2 cut(s) 314, 503
BpuEI CTTGAG 3 cut(s) 308, 554, 556
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 5 cut(s) 42, 63, 290, 390, 474
BsaWI WCCGGW 1 cut(s) 558
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 116, 146
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 439, 481
Bse1I ACTGG 1 cut(s) 260
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 5 cut(s) 42, 63, 290, 390, 474
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 3 cut(s) 64, 439, 481
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 4 cut(s) 124, 174, 184, 331
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 2 cut(s) 282, 298
BseYI CCCAGC 1 cut(s) 438
Bsh1285I CGRYCG 2 cut(s) 374, 563
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 2 cut(s) 374, 563
BsiHKAI GWGCWC 3 cut(s) 223, 513, 570
BsiSI CCGG 3 cut(s) 290, 405, 559
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 3 cut(s) 64, 439, 481
BsmAI GTCTC 1 cut(s) 318
BsmBI CGTCTC 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 4 cut(s) 223, 364, 513, 570
Bsp143I GATC 4 cut(s) 410, 447, 478, 515
Bsp19I CCATGG 2 cut(s) 42, 390
BspACI CCGC 3 cut(s) 243, 387, 605
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 5 cut(s) 48, 69, 412, 431, 517
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 6 cut(s) 405, 418, 455, 486, 510, 523
BsrI ACTGG 1 cut(s) 260
BssECI CCNNGG 5 cut(s) 42, 63, 290, 390, 474
BssMI GATC 4 cut(s) 410, 447, 478, 515
BssT1I CCWWGG 2 cut(s) 42, 390
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 64, 372
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 2 cut(s) 267, 365
BstDSI CCRYGG 3 cut(s) 42, 63, 390
BstF5I GGATG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 4 cut(s) 413, 450, 481, 518
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 410, 447, 478, 515
BstMCI CGRYCG 2 cut(s) 374, 563
BstMWI GCNNNNNNNGC 4 cut(s) 110, 292, 295, 304
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 545
BstPAI GACNNNNGTC 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 289, 474
BstSLI GKGCMC 1 cut(s) 364
BstV1I GCAGC 2 cut(s) 282, 298
BstX2I RGATCY 3 cut(s) 410, 478, 515
BstYI RGATCY 3 cut(s) 410, 478, 515
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 3 cut(s) 42, 63, 390
BtsCI GGATG 1 cut(s) 178
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 4 cut(s) 46, 67, 430, 639
Csp6I GTAC 2 cut(s) 400, 619
CviAII CATG 7 cut(s) 43, 357, 391, 444, 451, 542, 580
CviQI GTAC 2 cut(s) 400, 619
DdeI CTNAG 2 cut(s) 267, 365
DpnI GATC 4 cut(s) 412, 449, 480, 517
DpnII GATC 4 cut(s) 410, 447, 478, 515
EciI GGCGGA 1 cut(s) 376
Ecl136II GAGCTC 2 cut(s) 221, 568
Eco130I CCWWGG 2 cut(s) 42, 390
Eco24I GRGCYC 2 cut(s) 223, 570
Eco47I GGWCC 3 cut(s) 46, 430, 639
Eco53kI GAGCTC 2 cut(s) 221, 568
EcoICRI GAGCTC 2 cut(s) 221, 568
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 2 cut(s) 42, 390
EcoT38I GRGCYC 2 cut(s) 223, 570
ErhI CCWWGG 2 cut(s) 42, 390
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 7 cut(s) 46, 360, 394, 447, 454, 545, 583
FaiI YATR 9 cut(s) 44, 99, 358, 392, 416, 445, 452, 543, 581
FaqI GGGAC 1 cut(s) 304
FatI CATG 7 cut(s) 42, 356, 390, 443, 450, 541, 579
FauI CCCGC 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 287, 296, 605
FokI GGATG 1 cut(s) 165
FriOI GRGCYC 2 cut(s) 223, 570
Fsp4HI GCNGC 3 cut(s) 287, 296, 605
FspI TGCGCA 1 cut(s) 299
GlaI GCGC 1 cut(s) 299
GluI GCNGC 3 cut(s) 287, 296, 605
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 2 cut(s) 314, 503
HaeIII GGCC 1 cut(s) 68
HapII CCGG 3 cut(s) 290, 405, 559
HhaI GCGC 1 cut(s) 300
Hin1II CATG 7 cut(s) 46, 360, 394, 447, 454, 545, 583
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 532
HpaII CCGG 3 cut(s) 290, 405, 559
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 2 cut(s) 109, 658
Hpy188I TCNGA 7 cut(s) 218, 231, 515, 520, 565, 589, 596
Hpy188III TCNNGA 6 cut(s) 79, 269, 433, 456, 482, 571
Hpy8I GTNNAC 2 cut(s) 109, 658
HpyCH4III ACNGT 2 cut(s) 64, 372
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 1 cut(s) 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 292, 295, 304
HpyF3I CTNAG 2 cut(s) 267, 365
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 7 cut(s) 46, 360, 394, 447, 454, 545, 583
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 4 cut(s) 410, 447, 478, 515
LmnI GCTCC 5 cut(s) 101, 218, 271, 333, 565
Lsp1109I GCAGC 2 cut(s) 282, 298
MaeII ACGT 1 cut(s) 555
MalI GATC 4 cut(s) 412, 449, 480, 517
MboI GATC 4 cut(s) 410, 447, 478, 515
MboII GAAGA 2 cut(s) 28, 509
MflI RGATCY 3 cut(s) 410, 478, 515
MhlI GDGCHC 4 cut(s) 223, 364, 513, 570
MluCI AATT 4 cut(s) 19, 26, 225, 275
MmeI TCCRAC 3 cut(s) 543, 543, 574
MseI TTAA 2 cut(s) 30, 278
MspI CCGG 3 cut(s) 290, 405, 559
MspR9I CCNGG 2 cut(s) 291, 476
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 4 cut(s) 110, 292, 295, 304
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 2 cut(s) 42, 390
NdeII GATC 4 cut(s) 410, 447, 478, 515
NlaIII CATG 7 cut(s) 46, 360, 394, 447, 454, 545, 583
NlaIV GGNNCC 5 cut(s) 48, 69, 412, 431, 517
NsbI TGCGCA 1 cut(s) 299
NspI RCATGY 1 cut(s) 545
PflMI CCANNNNNTGG 1 cut(s) 481
PkrI GCNGC 3 cut(s) 288, 297, 606
PshAI GACNNNNGTC 1 cut(s) 307
Psp124BI GAGCTC 2 cut(s) 223, 570
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 5 cut(s) 48, 69, 412, 431, 517
PspPI GGNCC 4 cut(s) 46, 67, 430, 639
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 3 cut(s) 410, 478, 515
RsaI GTAC 2 cut(s) 401, 620
RsaNI GTAC 2 cut(s) 400, 619
SacI GAGCTC 2 cut(s) 223, 570
SaqAI TTAA 2 cut(s) 30, 278
SatI GCNGC 3 cut(s) 287, 296, 605
Sau3AI GATC 4 cut(s) 410, 447, 478, 515
Sau96I GGNCC 4 cut(s) 46, 67, 430, 639
ScrFI CCNGG 2 cut(s) 291, 476
SduI GDGCHC 4 cut(s) 223, 364, 513, 570
SinI GGWCC 3 cut(s) 46, 430, 639
SmlI CTYRAG 3 cut(s) 323, 535, 569
SmoI CTYRAG 3 cut(s) 323, 535, 569
Sse9I AATT 4 cut(s) 19, 26, 225, 275
SsiI CCGC 3 cut(s) 243, 387, 605
SstI GAGCTC 2 cut(s) 223, 570
StyD4I CCNGG 2 cut(s) 289, 474
StyI CCWWGG 2 cut(s) 42, 390
TaaI ACNGT 2 cut(s) 64, 372
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 223, 374
TasI AATT 4 cut(s) 19, 26, 225, 275
TauI GCSGC 1 cut(s) 607
Tru1I TTAA 2 cut(s) 30, 278
Tru9I TTAA 2 cut(s) 30, 278
TseI GCWGC 2 cut(s) 286, 295
TspDTI ATGAA 2 cut(s) 345, 503
Van91I CCANNNNNTGG 1 cut(s) 481
VpaK11BI GGWCC 3 cut(s) 46, 430, 639
XapI RAATTY 3 cut(s) 19, 26, 225
XceI RCATGY 1 cut(s) 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.