pycom05g21870

hemK methyltransferase family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009637
Physical Location & Seq
Forward (+)
24441519 .. 24442271
753 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21870.1

Sequence Viewer

Length: 753 bp
ATGTCCTCCAGAATCGCCCAAATCCGCCTTGTCAGCTCTCATCCCGAGGTTTATGAACCGTGTGATGATTCGTTTGCTTTAGTCGATGCTCTTTTAGCTGATCGAACAAACCTGTTGCAGCACCAACCGACATTGTGCTTGGAACTGGGTTGCGGTAGTGGCTATGTCATTACTTCTCTAGCTCTTATTGTTGGCCAGGCAGCGCATTATATTGCGACTGATATTAACCCCCACGCAGTGAAGGTGACTCGTGAGACATTAGAAGCACATGGGGTTCATGCGGAGTTGATAAACACTAACATTGCATCAGGACTGGAGAAGCGTCTGAAAGGTTTGGTTGATGTAATAGTTGTGAACCCGCCTTATGTGCCGACCCCTGAAGATGAGGTGGGACGTGAAGGGATTGCGTCTGCTTGGGCTGGGGGGGAGAATGGACGTACTGTGATTGATAGGATACTGCCTGTTTCTGACAATCTTTTGTCAAAGAAGGGCTGGTTGTACATGGTCACTCTTACAGAAAACAATCCCTTGCAGATATGCCTTCAAATGAGAGAAAAGGGCTATGCTTCCAGAATCCTTGTCCAGAGATTGACAGAGGAAGAAAATCTACAAATCATCAAGTTCTGGCGGGATTCAGATTCTCAAGTAGCAGAAAAGGAAAACAAAACAGTTCCTGCAAAGGTCATGGAGTCTTTGCTCTCTCAGTTCCATATATTCCCGTTTCTGAGAGGCAGTAACAACGACAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS

Protein Analysis

251

Amino Acids

27.66

Weight (kDa)

5.39

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTS PF05175 43 - 127 6.7e-12 Methyltransferase small domain
PrmA PF06325 43 - 119 1.5e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 46 - 119 1.6e-08 Methyltransferase domain
Methyltransf_25 PF13649 47 - 118 5.3e-08 Methyltransferase domain
Methyltransf_12 PF08242 47 - 118 7.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 25, 153, 281, 359, 628
AcoI YGGCCR 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 399
AdeI CACNNNGTG 1 cut(s) 238
AfaI GTAC 2 cut(s) 439, 500
AgsI TTSAA 1 cut(s) 545
AjiI CACGTC 1 cut(s) 395
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 4 cut(s) 36, 98, 182, 750
AluI AGCT 4 cut(s) 36, 98, 182, 750
Alw26I GTCTC 1 cut(s) 248
AlwNI CAGNNNCTG 2 cut(s) 674, 750
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 118, 200
AspLEI GCGC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 44
BalI TGGCCA 1 cut(s) 195
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BauI CACGAG 1 cut(s) 249
BbvI GCAGC 2 cut(s) 130, 212
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 1 cut(s) 179
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 2 cut(s) 119, 201
BlsI GCNGC 2 cut(s) 120, 202
Bme1390I CCNGG 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 44
BmgBI CACGTC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 2 cut(s) 76, 314
BmuI ACTGGG 1 cut(s) 155
BpmI CTGGAG 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 627
BsaJI CCNNGG 1 cut(s) 45
Bse1I ACTGG 2 cut(s) 150, 318
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 2 cut(s) 716, 716
BseNI ACTGG 2 cut(s) 150, 318
BseXI GCAGC 2 cut(s) 130, 212
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 405
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 5 cut(s) 25, 153, 281, 359, 628
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 715, 717
BsrDI GCAATG 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 150, 318
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 100
BssSI CACGAG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 4 cut(s) 60, 442, 670, 746
BstAUI TGTACA 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 702, 725
BstF5I GGATG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 4 cut(s) 33, 95, 159, 367
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 130, 212
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 1 cut(s) 195
BtrI CACGTC 1 cut(s) 395
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
CaiI CAGNNNCTG 2 cut(s) 674, 750
CfoI GCGC 1 cut(s) 205
CseI GACGC 2 cut(s) 311, 396
Csp6I GTAC 2 cut(s) 438, 499
CviAII CATG 4 cut(s) 269, 278, 502, 685
CviJI RGCY 9 cut(s) 36, 98, 162, 182, 195, 419, 492, 561, 750
CviKI_1 RGCY 9 cut(s) 36, 98, 162, 182, 195, 419, 492, 561, 750
CviQI GTAC 2 cut(s) 438, 499
DdeI CTNAG 2 cut(s) 702, 725
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DraIII CACNNNGTG 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 193
EciI GGCGGA 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 195
FaeI CATG 4 cut(s) 272, 281, 505, 688
FaqI GGGAC 1 cut(s) 405
FatI CATG 4 cut(s) 268, 277, 501, 684
FauI CCCGC 2 cut(s) 366, 621
Fnu4HI GCNGC 2 cut(s) 119, 201
FokI GGATG 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 119, 201
FspBI CTAG 1 cut(s) 179
GlaI GCGC 1 cut(s) 204
GluI GCNGC 2 cut(s) 119, 201
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 1 cut(s) 195
HgaI GACGC 2 cut(s) 311, 396
HhaI GCGC 1 cut(s) 205
Hin1II CATG 4 cut(s) 272, 281, 505, 688
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HinfI GANTC 7 cut(s) 12, 68, 247, 573, 632, 638, 689
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 4 cut(s) 327, 469, 637, 726
Hpy188III TCNNGA 6 cut(s) 9, 44, 251, 309, 570, 583
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 4 cut(s) 235, 392, 481, 551
HpyCH4III ACNGT 4 cut(s) 60, 442, 670, 746
HpyCH4IV ACGT 2 cut(s) 394, 436
HpyCH4V TGCA 4 cut(s) 118, 305, 532, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 95, 159, 367
HpyF3I CTNAG 2 cut(s) 702, 725
HpySE526I ACGT 2 cut(s) 394, 436
Hsp92II CATG 4 cut(s) 272, 281, 505, 688
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 1 cut(s) 100
Lsp1109I GCAGC 2 cut(s) 130, 212
LweI GCATC 2 cut(s) 76, 314
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 394, 436
MaeIII GTNAC 3 cut(s) 244, 505, 734
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 2 cut(s) 392, 611
MlsI TGGCCA 1 cut(s) 195
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 2 cut(s) 241, 698
MnlI CCTC 5 cut(s) 16, 40, 379, 589, 722
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
MseI TTAA 1 cut(s) 225
Msp20I TGGCCA 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 4 cut(s) 33, 95, 159, 367
NdeII GATC 1 cut(s) 100
NlaIII CATG 4 cut(s) 272, 281, 505, 688
NmuCI GTSAC 2 cut(s) 244, 505
PfeI GAWTC 5 cut(s) 12, 68, 573, 632, 638
PkrI GCNGC 2 cut(s) 120, 202
PleI GAGTC 2 cut(s) 241, 697
PpsI GAGTC 2 cut(s) 241, 697
Psp6I CCWGG 1 cut(s) 195
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 195
PstNI CAGNNNCTG 2 cut(s) 674, 750
RsaI GTAC 2 cut(s) 439, 500
RsaNI GTAC 2 cut(s) 438, 499
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 119, 201
Sau3AI GATC 1 cut(s) 100
SchI GAGTC 2 cut(s) 241, 698
ScrFI CCNGG 1 cut(s) 197
SfaNI GCATC 2 cut(s) 76, 314
SmlI CTYRAG 1 cut(s) 642
SmoI CTYRAG 1 cut(s) 642
SsiI CCGC 5 cut(s) 25, 153, 281, 359, 628
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 195
TaaI ACNGT 4 cut(s) 60, 442, 670, 746
TaiI ACGT 2 cut(s) 397, 439
TaqI TCGA 2 cut(s) 84, 103
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 5 cut(s) 12, 68, 573, 632, 638
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 2 cut(s) 244, 505
TseI GCWGC 2 cut(s) 118, 200
Tsp45I GTSAC 2 cut(s) 244, 505
TspDTI ATGAA 2 cut(s) 69, 266
TspRI CASTG 1 cut(s) 243
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.