Rorug05G0071400

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002001
Physical Location & Seq
Reverse (-)
6139925 .. 6142138
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0071400.1

Sequence Viewer

Length: 1077 bp
ATGGCCGAAATGAGGATGATGATGATTAGTATGACAATACTAACTTTGCTGCCTTTGCTCTCTGGAGCTTTGGATGTTGCTGAGATGAGGAAAGTCGCTGCTCGAAACAATGTGACATGTATCTTAGTATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAACGTTCTGCACACGACCATGAAAGGGAATTTCCTTCCTTATGGCAAAGAATTCTTCAATGGCCGCCCAACTGGAAGATTCAGTAATGGCAGACTTGCCACAGATTTTATTGCGGACGCAGTTGGGTATACAAAAACAATTCCAGCATTTCTTGATCCAAATTTAAAGCCTGCAGATCTGCCTCATGGTGTTAGTTTTGCATCAGCTGCCTCGGGTTATGACGACCTCACTGCCAATTTTTCGAATGTGTTACCTCTTTCCAGACAGCTGAAGAGTTTTATGCATTATAAATTAAACTTGACAAGATTGGTGGGTAAGAAGACTGCTGAAGATACCATAAAAAATGCCTTATTTGTTATGAGTATGGGAACAAATGACTTTATCCAAAACTACTACTTGGAACCAACTCGCTCCAAGCAGTTCACTGTGGAACAGTATCAAAATTATTTGGTCTCTTGCATGGCTCATTCCATTCAGGCAATGCATAGGCTAGGAGGCACAAGATTGGCTGTTGTTGGAGTTCCACCATTGGGGTGCATGCCACTTGTCAAAACGCTTATGGGCGAGACCAAATGTGTGGAAAATTATAACAAAGTGTCATTCTCTTTTAATTCCAAGATTCAAAAGAAATTAGAGAACATTACCAAAACATCTGGAATGAAGATTGCCTTTGTTGATGCATATGGTATTGTCGAAAGTGCCATGAATAACCCACAGTTATATGGTCTAACTGAAACTTCAAAAGGGTGTTGTGGGACTGGAACTATAGAGTTTGGAGAATCATGCAGAGGATTACAAACATGCACTGATCCAGCAAAGTATGTATTCTGGGATGCTGTTCATCCAACAGAGAGAATGTACGAAATCGTTGCTGAGAAAGCTTTGCAAACTCTTGATGGAAAACTCTCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

358

Amino Acids

39.45

Weight (kDa)

8.65

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 347 2.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 450, 750
AasI GACNNNNNNGTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 290
AciI CCGC 2 cut(s) 226, 275
AclI AACGTT 1 cut(s) 165
AclWI GGATC 3 cut(s) 143, 311, 965
AcoI YGGCCR 2 cut(s) 3, 223
AcsI RAATTY 3 cut(s) 190, 212, 322
AcuI CTGAAG 2 cut(s) 452, 510
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 3 cut(s) 12, 186, 692
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 3 cut(s) 220, 785, 903
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 4 cut(s) 68, 368, 430, 1043
AluI AGCT 4 cut(s) 68, 368, 430, 1043
Alw26I GTCTC 3 cut(s) 129, 619, 722
AlwI GGATC 3 cut(s) 143, 311, 965
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 3, 223
ApeKI GCWGC 3 cut(s) 49, 98, 368
ApoI RAATTY 3 cut(s) 190, 212, 322
AsuII TTCGAA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 36, 85, 355
BccI CCATC 1 cut(s) 1052
BcgI CGANNNNNNTGC 4 cut(s) 374, 408, 1013, 1047
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 3 cut(s) 129, 619, 722
BfaI CTAG 2 cut(s) 653, 1075
BfmI CTRYAG 2 cut(s) 333, 927
BglII AGATCT 1 cut(s) 337
BisI GCNGC 4 cut(s) 50, 99, 226, 369
BlsI GCNGC 4 cut(s) 51, 100, 227, 370
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 373
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 3 cut(s) 371, 829, 985
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 2 cut(s) 84, 124
Bpu14I TTCGAA 1 cut(s) 404
BsaI GGTCTC 2 cut(s) 619, 722
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 186, 692
Bse1I ACTGG 3 cut(s) 141, 238, 925
Bse3DI GCAATG 1 cut(s) 648
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 4 cut(s) 21, 79, 1000, 1003
BseLI CCNNNNNNNGG 3 cut(s) 12, 186, 692
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 2 cut(s) 72, 1026
BseNI ACTGG 3 cut(s) 141, 238, 925
BseXI GCAGC 3 cut(s) 36, 85, 355
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 2 cut(s) 5, 225
BsiHKCI CYCGRG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 3 cut(s) 12, 186, 692
BsmAI GTCTC 3 cut(s) 129, 619, 722
BsmFI GGGAC 1 cut(s) 931
BsnI GGCC 2 cut(s) 5, 225
Bso31I GGTCTC 2 cut(s) 619, 722
BsoBI CYCGRG 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 404
Bsp143I GATC 4 cut(s) 148, 316, 337, 970
BspACI CCGC 2 cut(s) 226, 275
BspANI GGCC 2 cut(s) 5, 225
BspCNI CTCAG 2 cut(s) 73, 1027
BspLI GGNNCC 1 cut(s) 564
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 3 cut(s) 143, 311, 965
BspT104I TTCGAA 1 cut(s) 404
BspTNI GGTCTC 2 cut(s) 619, 722
BsrDI GCAATG 1 cut(s) 648
BsrI ACTGG 3 cut(s) 141, 238, 925
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 4 cut(s) 148, 316, 337, 970
BssNAI GTATAC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 3 cut(s) 589, 597, 879
Bst6I CTCTTC 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 2 cut(s) 333, 701
BstDEI CTNAG 3 cut(s) 81, 124, 1035
BstF5I GGATG 4 cut(s) 21, 79, 1000, 1003
BstKTI GATC 4 cut(s) 151, 319, 340, 973
BstMAI GTCTC 3 cut(s) 129, 619, 722
BstMBI GATC 4 cut(s) 148, 316, 337, 970
BstMWI GCNNNNNNNGC 3 cut(s) 55, 368, 1040
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 3 cut(s) 120, 703, 966
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 2 cut(s) 333, 927
BstV1I GCAGC 3 cut(s) 36, 85, 355
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 739
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 5, 225
BtsCI GGATG 4 cut(s) 21, 79, 1000, 1003
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 4 cut(s) 148, 390, 585, 966
Cac8I GCNNGC 2 cut(s) 333, 701
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 1021
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 8 cut(s) 117, 181, 347, 622, 700, 865, 945, 963
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 3 cut(s) 81, 124, 1035
DpnI GATC 4 cut(s) 150, 318, 339, 972
DpnII GATC 4 cut(s) 148, 316, 337, 970
DraI TTTAAA 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 3, 223
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
Eco31I GGTCTC 2 cut(s) 619, 722
Eco57I CTGAAG 2 cut(s) 452, 510
Eco88I CYCGRG 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 151
EcoT22I ATGCAT 3 cut(s) 447, 648, 844
FaeI CATG 8 cut(s) 120, 184, 350, 625, 703, 868, 948, 966
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 931
FatI CATG 8 cut(s) 116, 180, 346, 621, 699, 864, 944, 962
FauNDI CATATG 1 cut(s) 844
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 50, 99, 226, 369
FokI GGATG 4 cut(s) 28, 86, 990, 1007
Fsp4HI GCNGC 4 cut(s) 50, 99, 226, 369
FspBI CTAG 2 cut(s) 653, 1075
GluI GCNGC 4 cut(s) 50, 99, 226, 369
GsuI CTGGAG 2 cut(s) 84, 124
HaeIII GGCC 2 cut(s) 5, 225
HgaI GACGC 1 cut(s) 287
Hin1II CATG 8 cut(s) 120, 184, 350, 625, 703, 868, 948, 966
HindIII AAGCTT 1 cut(s) 1041
HinfI GANTC 4 cut(s) 137, 240, 781, 941
Hpy166II GTNNAC 2 cut(s) 291, 585
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 5 cut(s) 63, 314, 423, 816, 1055
Hpy8I GTNNAC 2 cut(s) 291, 585
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 3 cut(s) 589, 597, 879
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 368, 1040
HpyF3I CTNAG 3 cut(s) 81, 124, 1035
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 8 cut(s) 120, 184, 350, 625, 703, 868, 948, 966
Kzo9I GATC 4 cut(s) 148, 316, 337, 970
LmnI GCTCC 2 cut(s) 65, 578
Lsp1109I GCAGC 3 cut(s) 36, 85, 355
LweI GCATC 3 cut(s) 371, 829, 985
MaeI CTAG 2 cut(s) 653, 1075
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 112, 411
MalI GATC 4 cut(s) 150, 318, 339, 972
MboI GATC 4 cut(s) 148, 316, 337, 970
MboII GAAGA 6 cut(s) 208, 249, 445, 493, 503, 835
MflI RGATCY 1 cut(s) 337
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 658, 1031
MnlI CCTC 8 cut(s) 6, 81, 354, 382, 398, 426, 650, 944
Mph1103I ATGCAT 3 cut(s) 447, 648, 844
MseI TTAA 3 cut(s) 326, 455, 771
MslI CAYNNNNRTG 2 cut(s) 179, 694
MspA1I CMGCKG 2 cut(s) 368, 430
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 55, 368, 1040
NdeI CATATG 1 cut(s) 844
NdeII GATC 4 cut(s) 148, 316, 337, 970
NlaIII CATG 8 cut(s) 120, 184, 350, 625, 703, 868, 948, 966
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 3 cut(s) 447, 648, 844
NspI RCATGY 3 cut(s) 120, 703, 966
NspV TTCGAA 1 cut(s) 404
PaeI GCATGC 1 cut(s) 703
PciI ACATGT 1 cut(s) 116
PfeI GAWTC 3 cut(s) 240, 781, 941
PkrI GCNGC 4 cut(s) 51, 100, 227, 370
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 1 cut(s) 116
PsiI TTATAA 2 cut(s) 450, 750
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 564
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 2 cut(s) 368, 430
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
RseI CAYNNNNRTG 2 cut(s) 179, 694
SaqAI TTAA 3 cut(s) 326, 455, 771
SatI GCNGC 4 cut(s) 50, 99, 226, 369
Sau3AI GATC 4 cut(s) 148, 316, 337, 970
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 153
SetI ASST 7 cut(s) 70, 168, 370, 390, 418, 432, 1045
SfaNI GCATC 3 cut(s) 371, 829, 985
SfcI CTRYAG 2 cut(s) 333, 927
SfuI TTCGAA 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 179, 694
SphI GCATGC 1 cut(s) 703
SsiI CCGC 2 cut(s) 226, 275
SspMI CTAG 2 cut(s) 653, 1075
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 3 cut(s) 589, 597, 879
TaiI ACGT 1 cut(s) 168
TaqI TCGA 4 cut(s) 103, 147, 404, 855
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 3 cut(s) 240, 781, 941
Tru1I TTAA 3 cut(s) 326, 455, 771
Tru9I TTAA 3 cut(s) 326, 455, 771
TscAI CASTG 4 cut(s) 148, 397, 592, 973
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 49, 98, 368
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 4 cut(s) 197, 836, 881, 992
TspRI CASTG 4 cut(s) 148, 397, 592, 973
XapI RAATTY 3 cut(s) 190, 212, 322
XceI RCATGY 3 cut(s) 120, 703, 966
XmiI GTMKAC 1 cut(s) 290
XspI CTAG 2 cut(s) 653, 1075
Zsp2I ATGCAT 3 cut(s) 447, 648, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.