RchiOBHm_Chr4g0431171

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002001
Physical Location & Seq
Forward (+)
55465469 .. 55467831
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39987

Sequence Viewer

Length: 492 bp
ATGGCAAAGACGCCCGCAAAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTATTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCACTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGGGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTTTCTATGGTATGGGATGCACTCCAGACTATGTTGTTTATCATGAACTGATTTTGACTATGAAGGAGTACATGCAGTGTGCCACTGCTGTGGAGCCACAGTGGTTGGTCGAGTTGGGGCCCATCTTCTTCTCTGTGAAGGATTCTGATACATCGCTGTTGGAGCATAAGAAGAGACAGCAATGGAGCAAGAGATGGAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

163

Amino Acids

18.93

Weight (kDa)

9.42

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 59 - 136 6.6e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 15
AclWI GGATC 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 2 cut(s) 21, 255
AgsI TTSAA 2 cut(s) 127, 223
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 2 cut(s) 112, 185
AluI AGCT 2 cut(s) 112, 185
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 1 cut(s) 99
AoxI GGCC 2 cut(s) 158, 404
ApaI GGGCCC 1 cut(s) 408
ApeKI GCWGC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 58, 404, 405
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 408
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BanII GRGCYC 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 99
BccI CCATC 3 cut(s) 276, 416, 474
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 3 cut(s) 93, 116, 216
BglII AGATCT 1 cut(s) 120
BisI GCNGC 1 cut(s) 113
BlsI GCNGC 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 58, 404, 405
BmiI GGNNCC 4 cut(s) 59, 381, 405, 406
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 249, 293
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 255
Bse1I ACTGG 2 cut(s) 53, 259
Bse3DI GCAATG 1 cut(s) 473
BseGI GGATG 3 cut(s) 264, 274, 308
BseLI CCNNNNNNNGG 2 cut(s) 21, 255
BseMI GCAATG 1 cut(s) 473
BseNI ACTGG 2 cut(s) 53, 259
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 99
BseYI CCCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 160, 406
BslFI GGGAC 2 cut(s) 59, 71
BslI CCNNNNNNNGG 2 cut(s) 21, 255
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 2 cut(s) 59, 71
BsnI GGCC 2 cut(s) 160, 406
Bsp120I GGGCCC 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 2 cut(s) 104, 120
BspACI CCGC 1 cut(s) 15
BspANI GGCC 2 cut(s) 160, 406
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 4 cut(s) 59, 381, 405, 406
BspPI GGATC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 473
BsrI ACTGG 2 cut(s) 53, 259
BssMI GATC 2 cut(s) 104, 120
BssNI GRCGYC 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 452
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 15
BstF5I GGATG 3 cut(s) 264, 274, 308
BstKTI GATC 2 cut(s) 107, 123
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 2 cut(s) 104, 120
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstNSI RCATGY 2 cut(s) 74, 361
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 2 cut(s) 104, 120
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 2 cut(s) 104, 120
BsuRI GGCC 2 cut(s) 160, 406
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 3 cut(s) 264, 274, 308
BtsI GCAGTG 3 cut(s) 289, 368, 369
BtsIMutI CAGTG 7 cut(s) 131, 143, 165, 289, 368, 369, 392
Cac8I GCNNGC 1 cut(s) 15
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 58, 404, 405
CseI GACGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 355
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 4 cut(s) 71, 264, 329, 358
CviJI RGCY 6 cut(s) 92, 112, 160, 185, 382, 406
CviKI_1 RGCY 6 cut(s) 92, 112, 160, 185, 382, 406
CviQI GTAC 1 cut(s) 355
DpnI GATC 2 cut(s) 106, 122
DpnII GATC 2 cut(s) 104, 120
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 408
FaeI CATG 4 cut(s) 74, 267, 332, 361
FaqI GGGAC 2 cut(s) 59, 71
FatI CATG 4 cut(s) 70, 263, 328, 357
FauI CCCGC 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 113
FokI GGATG 3 cut(s) 261, 271, 315
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 113
FspBI CTAG 3 cut(s) 93, 116, 216
GluI GCNGC 1 cut(s) 113
GsaI CCCAGC 1 cut(s) 112
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 160, 406
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 4 cut(s) 74, 267, 332, 361
HinfI GANTC 2 cut(s) 428, 486
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 279, 433, 491
Hpy188III TCNNGA 4 cut(s) 124, 249, 311, 329
HpyAV CCTTC 3 cut(s) 217, 343, 418
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 70, 197, 306, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 74, 267, 332, 361
Kzo9I GATC 2 cut(s) 104, 120
LmnI GCTCC 3 cut(s) 379, 448, 471
LpnPI CCDG 6 cut(s) 34, 122, 174, 240, 262, 324
Lsp1109I GCAGC 1 cut(s) 99
LweI GCATC 2 cut(s) 249, 293
MaeI CTAG 3 cut(s) 93, 116, 216
MaeIII GTNAC 1 cut(s) 81
MalI GATC 2 cut(s) 106, 122
MboI GATC 2 cut(s) 104, 120
MboII GAAGA 4 cut(s) 139, 403, 406, 469
MflI RGATCY 2 cut(s) 104, 120
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 208, 244
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 2 cut(s) 35, 91
MseI TTAA 2 cut(s) 75, 243
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 448
NdeII GATC 2 cut(s) 104, 120
NlaIII CATG 4 cut(s) 74, 267, 332, 361
NlaIV GGNNCC 4 cut(s) 59, 381, 405, 406
NspI RCATGY 2 cut(s) 74, 361
OliI CACNNNNGTG 1 cut(s) 374
PagI TCATGA 1 cut(s) 328
PfeI GAWTC 2 cut(s) 428, 486
PkrI GCNGC 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 108
PspN4I GGNNCC 4 cut(s) 59, 381, 405, 406
PspOMI GGGCCC 1 cut(s) 404
PspPI GGNCC 3 cut(s) 58, 404, 405
PsuI RGATCY 2 cut(s) 104, 120
PvuII CAGCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 374
SaqAI TTAA 2 cut(s) 75, 243
SatI GCNGC 1 cut(s) 113
Sau3AI GATC 2 cut(s) 104, 120
Sau96I GGNCC 3 cut(s) 58, 404, 405
SduI GDGCHC 1 cut(s) 408
SetI ASST 3 cut(s) 102, 114, 187
SfaNI GCATC 2 cut(s) 249, 293
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 2 cut(s) 208, 244
SsiI CCGC 1 cut(s) 15
SspMI CTAG 3 cut(s) 93, 116, 216
TaaI ACNGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 40, 396
TasI AATT 2 cut(s) 208, 244
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 2 cut(s) 428, 486
Tru1I TTAA 2 cut(s) 75, 243
Tru9I TTAA 2 cut(s) 75, 243
TscAI CASTG 7 cut(s) 138, 150, 172, 289, 368, 376, 392
TseI GCWGC 1 cut(s) 112
TspDTI ATGAA 2 cut(s) 345, 362
TspRI CASTG 7 cut(s) 138, 150, 172, 289, 368, 376, 392
VpaK11BI GGWCC 1 cut(s) 58
XceI RCATGY 2 cut(s) 74, 361
XspI CTAG 3 cut(s) 93, 116, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.