pycom05g21860

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002001
Physical Location & Seq
Reverse (-)
24436239 .. 24439741
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21860.2

Sequence Viewer

Length: 2454 bp
ATGCGTCTAGAGATGGAATACAATTCTGATCATGCGTGGTACGATAGGGAAGAAGGAAACAAGATGTTTGATACAGTTGATAGCTCGTGTTTGTTTTTTGGAGATAATGCTTCTTTTCAGAAGAAGGAAGCAGAGTTGGCAAAAAGATTGGTTCGAAGAGATGGAACTAAGATGACCCTTGCTCAGAGCAAAAAGTTGTCTCAGCTCACTGCTGATAGTATGCGGTGGGAGGATCGTCAACTTTTAAGATCTGGTGCTGTTAGAGTGACTGAGGTGCAGACTGAGTTCGATGATGAAGAAGAGCACAAATGTTTTTGTAAAGGATGGAGTGTTGTTTTTACCAAGCGATCAGAGCCGATAATGCCAATAAAGGATCCCACGTCTGATATGGCCATAATTTCTCGCGAAGGGTCTGCTCTCATTAGGAAAATCCATGAGAAGCAAAATCTGAACAAATCGCGTCAGCGTTTTTGGGAGCTTGCAGGTTCTAAACTAGGCGATATCCTTGGTGTTGAGAAAGCACCGGAGCAGGTTGAAGCAGATACTGCTGCTGCAGGTGCAGACGGTGAAACTGATTTCAAAGAAGATGTAAAGTTTGCACAGCATATGAAGAAAGGGGAAGTAGCAAGTGAATTCACAAAGTCAAAAACTATCTCGCAGCAACAGCAGTTTTTGCCCATATATTCTATGAGGGATGATTTACTTCAGGTAATTCGAAAAAATCAAGTTATTGTTATTGTTGGAGAAACCGGTTCTGGAAAGACGACTCAACTGACACAGTATCTGCACGAAGAGGGATACACTATAAATGGCATTGTGGGTTGCACCCAACCAAGACGTGTGGCTGCCATGAGTGTTGCAAAGAGAGTTAGTGAAGAAATGGAGACTGAGCTTGGTGACAAAGTGGGGTACGCTATTCGCTTTGAGGATGTGATTGGAGCAAATATCATAATAAAGTACATGACTGATGGAATACTTATGTATAGTAAAACCCCATGTGAAGATTATGTTGAAGCTGCAGTGAAGGAGGCCATAACGATTCACATCACCAGCCCTCAAGGTGACATCCTCATCTTCATGACCGGCCAAGATGAGATCGAGGCAGCTTGTTATGCCCTATCAGAGCGGATGGAACAAATTATCTCCACCTTGGTTAAAGAGGTGCCAAAACTCTTGATACTTCCTATATATTCACAGTTGCCTGCTGACTTGCAAGCGAAGATATTCCGAAAAGCTGAAGATGGGGCTCGTAAATGCATTGTGGCAACCAACATTGTATACAACCCGAAAATGGGTATGGATGCTCTTCGAGTATTTCCTATTAGTCAGACTGCTGCTAATCAACGTGCTGGGCGTGCTGGCAGAACTAAGCCTGGCACATGTTATCGGCTATACACAGAGAATGAGTACTTAAACGAAATGCTACCCAATCCGGTGCCAGAGATCCAGTGGACCAATCTTGGATATGTGGTCTTGTTGCTCAAATCTCTTCAGGTTGATAACTTGCTAGATTTTGATTTCTTAGACCCACCTCCCAAGGATAATATTCTGCATTCTATGTACCAGTTGTGGGTGTTGGGTGCTCTTAACAACGAGGGGTGGTTAACTGACCTGGGCTGGAAAATGGTGGAGTTGCCATTGGACCCTCCACTCGCTAAGATGCTATTGATGGGTGAAAAGCTAGGATGTTTAGACGAGATTTTGACAATTGTGTCCATGCTTTCAGTGCCGTCGGTATTCTCTAGGCCTGAAGATAGGGCAGAGGAGAGCGATGCTGCAAGGGAGAGATTCTCCTTCCTAGAGTCCGACCACTTGACATTGTACAACATTTATCAGCAGTGGGAACAACATCAGTATCGTGGGGACTGGTACAATGACCATTTTTTGCAAGTTAAAGGGCTGCAAAAGGTTAGAGAGGTGAGGTCCCAACTTCTAGAGATACTGAAGACACTCAAAGTCCCGCTCACATCCTGTTGGCCTCATACTGATACCGTGAGAAGAGCTATTTGCTCCGCTTACTTTCGGAACGCAGCAAGATTGAAAGGTGTGGGGGAGTATGTCAATTGCCGGACTGGGATGCCATGCCATTTGCACCGGAGCAGCGCACTTTATGGTATGGGCTGCAATCCAGATTATGTGATCTATCATGAGTTAGTTTTGACAACGAAGGAGTATATGCAGTCACTTTTGCCTGCCATTGATAACCAGAACAAATCTACCTTCACTCTAACAAATACAACAACAAAACCAAAAAAAACCCCCAAAAATAAGGACCGACTGCCTTCTTGCTTTCATCACTTCTTACTTTGGCTGCCAGCTAGCTACTGCTATAGTACTACTCAACTGTGTTCGCGACTTGATCCAAAATCGTCTATTATTGTACATGTTCTTGACATTTTTGTTGCCGGGTCACCAAAATATTGTTGTTGCTACACGAAGCGATTCAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

818

Amino Acids

93.14

Weight (kDa)

6.43

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
AasI GACNNNNNNGTC 1 cut(s) 1711
Acc36I ACCTGC 3 cut(s) 473, 520, 545
AccB1I GGYRCC 2 cut(s) 1162, 1435
AccBSI CCGCTC 2 cut(s) 1126, 1963
AccI GTMKAC 1 cut(s) 1278
AccII CGCG 3 cut(s) 405, 460, 2353
AciI CCGC 4 cut(s) 223, 1126, 1961, 2013
AclWI GGATC 5 cut(s) 240, 368, 381, 1438, 2354
AcoI YGGCCR 2 cut(s) 390, 1084
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 5 cut(s) 689, 1257, 1475, 1770, 1964
AfaI GTAC 9 cut(s) 41, 911, 959, 1409, 1562, 1823, 1871, 2335, 2382
AfiI CCNNNNNNNGG 3 cut(s) 1291, 1292, 1570
AflIII ACRYGT 3 cut(s) 838, 1379, 2383
AgeI ACCGGT 1 cut(s) 749
AgsI TTSAA 5 cut(s) 536, 580, 1013, 2041, 2446
AjiI CACGTC 2 cut(s) 381, 839
AjnI CCWGG 2 cut(s) 1372, 1611
Alw21I GWGCWC 2 cut(s) 306, 1585
Alw26I GTCTC 2 cut(s) 204, 878
AlwI GGATC 5 cut(s) 240, 368, 381, 1438, 2354
AlwNI CAGNNNCTG 2 cut(s) 545, 784
AoxI GGCC 5 cut(s) 390, 1029, 1084, 1745, 1976
ApoI RAATTY 1 cut(s) 632
AsiGI ACCGGT 1 cut(s) 749
Asp700I GAANNNNTTC 2 cut(s) 1223, 2441
AspLEI GCGC 1 cut(s) 2105
AspS9I GGNCC 4 cut(s) 1452, 1642, 1923, 2272
AsuC2I CCSGG 1 cut(s) 2407
AsuHPI GGTGA 7 cut(s) 578, 908, 1039, 1073, 1685, 1930, 2403
AsuII TTCGAA 2 cut(s) 154, 715
AsuNHI GCTAGC 1 cut(s) 2318
AvaII GGWCC 4 cut(s) 1452, 1642, 1923, 2272
BaeI ACNNNNGTAYC 2 cut(s) 1838, 1871
BalI TGGCCA 1 cut(s) 392
BamHI GGATCC 1 cut(s) 373
BanI GGYRCC 2 cut(s) 1162, 1435
BanII GRGCYC 1 cut(s) 1249
BauI CACGAG 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 1952
Bbv12I GWGCWC 2 cut(s) 306, 1585
BccI CCATC 7 cut(s) 7, 155, 318, 962, 1123, 1235, 1663
BceAI ACGGC 1 cut(s) 1714
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 2 cut(s) 1374, 1613
BciVI GTATCC 1 cut(s) 791
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 1 cut(s) 2407
BcoDI GTCTC 2 cut(s) 204, 878
BfaI CTAG 8 cut(s) 8, 494, 1508, 1682, 1743, 1799, 1934, 2319
BfmI CTRYAG 3 cut(s) 552, 1017, 2329
BfuAI ACCTGC 3 cut(s) 473, 520, 545
BfuI GTATCC 1 cut(s) 791
BglII AGATCT 1 cut(s) 248
BmcAI AGTACT 2 cut(s) 1409, 2335
Bme1390I CCNGG 3 cut(s) 1374, 1613, 2407
Bme18I GGWCC 4 cut(s) 1452, 1642, 1923, 2272
BmgBI CACGTC 2 cut(s) 381, 839
BmgT120I GGNCC 4 cut(s) 1452, 1642, 1923, 2272
BmiI GGNNCC 5 cut(s) 375, 1164, 1437, 1644, 1925
BmrFI CCNGG 3 cut(s) 1374, 1613, 2407
BmrI ACTGGG 1 cut(s) 2082
BmsI GCATC 4 cut(s) 1291, 1650, 1762, 2067
BmtI GCTAGC 1 cut(s) 2322
BmuI ACTGGG 1 cut(s) 2082
BpiI GAAGAC 1 cut(s) 1952
BplI GAGNNNNNCTC 2 cut(s) 1775, 1807
Bpu14I TTCGAA 2 cut(s) 154, 715
BpuEI CTTGAG 1 cut(s) 1041
BpuMI CCSGG 1 cut(s) 2407
BsaBI GATNNNNATC 1 cut(s) 1043
BsaJI CCNNGG 4 cut(s) 505, 1149, 1536, 1612
BsaWI WCCGGW 4 cut(s) 523, 749, 1432, 2094
Bsc4I CCNNNNNNNGG 3 cut(s) 1291, 1292, 1570
Bse118I RCCGGY 2 cut(s) 749, 1082
Bse1I ACTGG 4 cut(s) 1447, 1564, 1871, 2077
Bse8I GATNNNNATC 1 cut(s) 1043
BseBI CCWGG 2 cut(s) 1374, 1613
BseDI CCNNGG 4 cut(s) 505, 1149, 1536, 1612
BseGI GGATG 9 cut(s) 329, 700, 934, 1065, 1134, 1306, 1691, 1967, 2082
BseJI GATNNNNATC 1 cut(s) 1043
BseLI CCNNNNNNNGG 3 cut(s) 1291, 1292, 1570
BseMII CTCAG 5 cut(s) 197, 215, 261, 273, 879
BseNI ACTGG 4 cut(s) 1447, 1564, 1871, 2077
BseRI GAGGAG 1 cut(s) 1778
BseYI CCCAGC 1 cut(s) 1349
BsgI GTGCAG 3 cut(s) 296, 579, 770
Bsh1236I CGCG 3 cut(s) 405, 460, 2353
BshFI GGCC 5 cut(s) 392, 1031, 1086, 1747, 1978
BshNI GGYRCC 2 cut(s) 1162, 1435
BshTI ACCGGT 1 cut(s) 749
BsiHKAI GWGCWC 2 cut(s) 306, 1585
BsiSI CCGG 7 cut(s) 524, 750, 1083, 1433, 2068, 2095, 2406
BslFI GGGAC 3 cut(s) 1877, 1909, 1943
BslI CCNNNNNNNGG 3 cut(s) 1291, 1292, 1570
BsmAI GTCTC 2 cut(s) 204, 878
BsmFI GGGAC 3 cut(s) 1877, 1909, 1943
BsmI GAATGC 1 cut(s) 1552
BsnI GGCC 5 cut(s) 392, 1031, 1086, 1747, 1978
Bsp119I TTCGAA 2 cut(s) 154, 715
Bsp1286I GDGCHC 3 cut(s) 306, 1249, 1585
Bsp1407I TGTACA 2 cut(s) 1821, 2380
Bsp143I GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
Bsp68I TCGCGA 2 cut(s) 405, 2353
BspACI CCGC 4 cut(s) 223, 1126, 1961, 2013
BspANI GGCC 5 cut(s) 392, 1031, 1086, 1747, 1978
BspCNI CTCAG 5 cut(s) 196, 214, 262, 274, 880
BspFNI CGCG 3 cut(s) 405, 460, 2353
BspHI TCATGA 2 cut(s) 1077, 2146
BspLI GGNNCC 5 cut(s) 375, 1164, 1437, 1644, 1925
BspMAI CTGCAG 2 cut(s) 556, 1021
BspMI ACCTGC 3 cut(s) 473, 520, 545
BspOI GCTAGC 1 cut(s) 2322
BspPI GGATC 5 cut(s) 240, 368, 381, 1438, 2354
BspQI GCTCTTC 3 cut(s) 294, 1311, 1993
BspT104I TTCGAA 2 cut(s) 154, 715
BspT107I GGYRCC 2 cut(s) 1162, 1435
BsrBI CCGCTC 2 cut(s) 1126, 1963
BsrFI RCCGGY 2 cut(s) 749, 1082
BsrGI TGTACA 2 cut(s) 1821, 2380
BsrI ACTGG 4 cut(s) 1447, 1564, 1871, 2077
BssAI RCCGGY 2 cut(s) 749, 1082
BssECI CCNNGG 4 cut(s) 505, 1149, 1536, 1612
BssMI GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
BssNAI GTATAC 1 cut(s) 1279
BssSI CACGAG 1 cut(s) 85
BssT1I CCWWGG 3 cut(s) 505, 1149, 1536
Bst1107I GTATAC 1 cut(s) 1279
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 1374, 1613
Bst4CI ACNGT 6 cut(s) 76, 566, 780, 1197, 1993, 2346
Bst6I CTCTTC 6 cut(s) 151, 294, 786, 1311, 1494, 1993
BstAPI GCANNNNNTGC 2 cut(s) 545, 673
BstAUI TGTACA 2 cut(s) 1821, 2380
BstBI TTCGAA 2 cut(s) 154, 715
BstC8I GCNNGC 8 cut(s) 480, 1203, 1215, 1356, 1360, 2193, 2316, 2320
BstDEI CTNAG 9 cut(s) 168, 183, 201, 270, 282, 888, 1368, 1522, 1656
BstEII GGTNACC 1 cut(s) 2409
BstF5I GGATG 9 cut(s) 329, 700, 934, 1065, 1134, 1306, 1691, 1967, 2082
BstFNI CGCG 3 cut(s) 405, 460, 2353
BstHHI GCGC 1 cut(s) 2105
BstKTI GATC 9 cut(s) 31, 235, 251, 350, 376, 1098, 1446, 2142, 2362
BstMAI GTCTC 2 cut(s) 204, 878
BstMBI GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
BstNI CCWGG 2 cut(s) 1374, 1613
BstNSI RCATGY 2 cut(s) 1383, 2387
BstPI GGTNACC 1 cut(s) 2409
BstSCI CCNGG 3 cut(s) 1372, 1611, 2405
BstSFI CTRYAG 3 cut(s) 552, 1017, 2329
BstUI CGCG 3 cut(s) 405, 460, 2353
BstV2I GAAGAC 1 cut(s) 1952
BstX2I RGATCY 3 cut(s) 248, 373, 1443
BstYI RGATCY 3 cut(s) 248, 373, 1443
BstZ17I GTATAC 1 cut(s) 1279
BsuI GTATCC 1 cut(s) 791
BsuRI GGCC 5 cut(s) 392, 1031, 1086, 1747, 1978
BtgZI GCGATG 1 cut(s) 1785
BtrI CACGTC 2 cut(s) 381, 839
BtsCI GGATG 9 cut(s) 329, 700, 934, 1065, 1134, 1306, 1691, 1967, 2082
BtsI GCAGTG 3 cut(s) 207, 1026, 1844
BtsIMutI CAGTG 5 cut(s) 207, 1026, 1454, 1731, 1844
BtuMI TCGCGA 2 cut(s) 405, 2353
BveI ACCTGC 3 cut(s) 473, 520, 545
Cac8I GCNNGC 8 cut(s) 480, 1203, 1215, 1356, 1360, 2193, 2316, 2320
CaiI CAGNNNCTG 2 cut(s) 545, 784
CciI TCATGA 2 cut(s) 1077, 2146
CfoI GCGC 1 cut(s) 2105
Cfr10I RCCGGY 2 cut(s) 749, 1082
Cfr13I GGNCC 4 cut(s) 1452, 1642, 1923, 2272
CseI GACGC 1 cut(s) 449
Csp6I GTAC 9 cut(s) 40, 910, 958, 1408, 1561, 1822, 1870, 2334, 2381
CspAI ACCGGT 1 cut(s) 749
CspCI CAANNNNNGTGG 2 cut(s) 822, 857
CviQI GTAC 9 cut(s) 40, 910, 958, 1408, 1561, 1822, 1870, 2334, 2381
DdeI CTNAG 9 cut(s) 168, 183, 201, 270, 282, 888, 1368, 1522, 1656
DpnI GATC 9 cut(s) 30, 234, 250, 349, 375, 1097, 1445, 2141, 2361
DpnII GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
DrdI GACNNNNNNGTC 1 cut(s) 1711
DseDI GACNNNNNNGTC 1 cut(s) 1711
EaeI YGGCCR 2 cut(s) 390, 1084
Eam1104I CTCTTC 6 cut(s) 151, 294, 786, 1311, 1494, 1993
EarI CTCTTC 6 cut(s) 151, 294, 786, 1311, 1494, 1993
Eco130I CCWWGG 3 cut(s) 505, 1149, 1536
Eco147I AGGCCT 1 cut(s) 1747
Eco24I GRGCYC 1 cut(s) 1249
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 4 cut(s) 1452, 1642, 1923, 2272
Eco57I CTGAAG 5 cut(s) 689, 1257, 1475, 1770, 1964
Eco91I GGTNACC 1 cut(s) 2409
EcoO109I RGGNCCY 1 cut(s) 1923
EcoO65I GGTNACC 1 cut(s) 2409
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 1372, 1611
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 3 cut(s) 505, 1149, 1536
EcoT22I ATGCAT 1 cut(s) 1259
EcoT38I GRGCYC 1 cut(s) 1249
ErhI CCWWGG 3 cut(s) 505, 1149, 1536
FaqI GGGAC 3 cut(s) 1877, 1909, 1943
FauI CCCGC 1 cut(s) 1968
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 1278
FokI GGATG 9 cut(s) 336, 707, 941, 1052, 1141, 1313, 1698, 1954, 2089
FriOI GRGCYC 1 cut(s) 1249
FspBI CTAG 8 cut(s) 8, 494, 1508, 1682, 1743, 1799, 1934, 2319
GlaI GCGC 1 cut(s) 2104
GsaI CCCAGC 1 cut(s) 1353
HaeIII GGCC 5 cut(s) 392, 1031, 1086, 1747, 1978
HapII CCGG 7 cut(s) 524, 750, 1083, 1433, 2068, 2095, 2406
HgaI GACGC 1 cut(s) 449
HhaI GCGC 1 cut(s) 2105
Hin6I GCGC 1 cut(s) 2103
HinP1I GCGC 1 cut(s) 2103
HincII GTYRAC 2 cut(s) 239, 1605
HindII GTYRAC 2 cut(s) 239, 1605
HinfI GANTC 5 cut(s) 766, 1039, 1788, 1802, 2442
HpaI GTTAAC 1 cut(s) 1605
HpaII CCGG 7 cut(s) 524, 750, 1083, 1433, 2068, 2095, 2406
HphI GGTGA 7 cut(s) 578, 908, 1039, 1073, 1685, 1930, 2403
Hpy166II GTNNAC 4 cut(s) 239, 1279, 1452, 1605
Hpy8I GTNNAC 4 cut(s) 239, 1279, 1452, 1605
Hpy99I CGWCG 1 cut(s) 1735
HpyAV CCTTC 8 cut(s) 47, 118, 401, 1018, 1804, 2161, 2230, 2292
HpyCH4III ACNGT 6 cut(s) 76, 566, 780, 1197, 1993, 2346
HpyCH4IV ACGT 3 cut(s) 380, 838, 1345
HpyF3I CTNAG 9 cut(s) 168, 183, 201, 270, 282, 888, 1368, 1522, 1656
HpySE526I ACGT 3 cut(s) 380, 838, 1345
HspAI GCGC 1 cut(s) 2103
Ksp22I TGATCA 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 1605
Kzo9I GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
LguI GCTCTTC 3 cut(s) 294, 1311, 1993
LmnI GCTCC 5 cut(s) 475, 526, 938, 2015, 2097
LweI GCATC 4 cut(s) 1291, 1650, 1762, 2067
MaeI CTAG 8 cut(s) 8, 494, 1508, 1682, 1743, 1799, 1934, 2319
MaeII ACGT 3 cut(s) 380, 838, 1345
MaeIII GTNAC 5 cut(s) 265, 896, 1061, 2181, 2409
MalI GATC 9 cut(s) 30, 234, 250, 349, 375, 1097, 1445, 2141, 2361
MbiI CCGCTC 2 cut(s) 1126, 1963
MboI GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
MfeI CAATTG 2 cut(s) 1707, 2062
MflI RGATCY 3 cut(s) 248, 373, 1443
MhlI GDGCHC 3 cut(s) 306, 1249, 1585
MlsI TGGCCA 1 cut(s) 392
MluCI AATT 7 cut(s) 22, 396, 632, 711, 1137, 1707, 2062
MluNI TGGCCA 1 cut(s) 392
MlyI GAGTC 2 cut(s) 760, 1811
MmeI TCCRAC 2 cut(s) 721, 1830
Mox20I TGGCCA 1 cut(s) 392
Mph1103I ATGCAT 1 cut(s) 1259
MroXI GAANNNNTTC 2 cut(s) 1223, 2441
MscI TGGCCA 1 cut(s) 392
MseI TTAA 6 cut(s) 245, 1155, 1412, 1587, 1604, 1893
MslI CAYNNNNRTG 1 cut(s) 1076
Msp20I TGGCCA 1 cut(s) 392
MspI CCGG 7 cut(s) 524, 750, 1083, 1433, 2068, 2095, 2406
MspR9I CCNGG 3 cut(s) 1374, 1613, 2407
MunI CAATTG 2 cut(s) 1707, 2062
Mva1269I GAATGC 1 cut(s) 1552
MvaI CCWGG 2 cut(s) 1374, 1613
MvnI CGCG 3 cut(s) 405, 460, 2353
NciI CCSGG 1 cut(s) 2407
NdeI CATATG 1 cut(s) 606
NdeII GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
NheI GCTAGC 1 cut(s) 2318
NlaIV GGNNCC 5 cut(s) 375, 1164, 1437, 1644, 1925
NmuCI GTSAC 5 cut(s) 265, 896, 1061, 2181, 2409
NruI TCGCGA 2 cut(s) 405, 2353
NsiI ATGCAT 1 cut(s) 1259
NspI RCATGY 2 cut(s) 1383, 2387
NspV TTCGAA 2 cut(s) 154, 715
PagI TCATGA 2 cut(s) 1077, 2146
PaqCI CACCTGC 1 cut(s) 545
PceI AGGCCT 1 cut(s) 1747
PciI ACATGT 2 cut(s) 1379, 2383
PciSI GCTCTTC 3 cut(s) 294, 1311, 1993
PcsI WCGNNNNNNNCGW 1 cut(s) 1351
PctI GAATGC 1 cut(s) 1552
PdmI GAANNNNTTC 2 cut(s) 1223, 2441
PfeI GAWTC 3 cut(s) 1039, 1788, 2442
PinAI ACCGGT 1 cut(s) 749
PleI GAGTC 2 cut(s) 760, 1810
PpsI GAGTC 2 cut(s) 760, 1810
PpuMI RGGWCCY 1 cut(s) 1923
PscI ACATGT 2 cut(s) 1379, 2383
Psp5II RGGWCCY 1 cut(s) 1923
Psp6I CCWGG 2 cut(s) 1372, 1611
PspEI GGTNACC 1 cut(s) 2409
PspFI CCCAGC 1 cut(s) 1349
PspGI CCWGG 2 cut(s) 1372, 1611
PspN4I GGNNCC 5 cut(s) 375, 1164, 1437, 1644, 1925
PspPI GGNCC 4 cut(s) 1452, 1642, 1923, 2272
PspPPI RGGWCCY 1 cut(s) 1923
PstI CTGCAG 2 cut(s) 556, 1021
PstNI CAGNNNCTG 2 cut(s) 545, 784
PsuI RGATCY 3 cut(s) 248, 373, 1443
RruI TCGCGA 2 cut(s) 405, 2353
RsaI GTAC 9 cut(s) 41, 911, 959, 1409, 1562, 1823, 1871, 2335, 2382
RsaNI GTAC 9 cut(s) 40, 910, 958, 1408, 1561, 1822, 1870, 2334, 2381
RseI CAYNNNNRTG 1 cut(s) 1076
SapI GCTCTTC 3 cut(s) 294, 1311, 1993
SaqAI TTAA 6 cut(s) 245, 1155, 1412, 1587, 1604, 1893
Sau3AI GATC 9 cut(s) 28, 232, 248, 347, 373, 1095, 1443, 2139, 2359
Sau96I GGNCC 4 cut(s) 1452, 1642, 1923, 2272
ScaI AGTACT 2 cut(s) 1409, 2335
SchI GAGTC 2 cut(s) 760, 1811
ScrFI CCNGG 3 cut(s) 1374, 1613, 2407
SduI GDGCHC 3 cut(s) 306, 1249, 1585
SfaNI GCATC 4 cut(s) 1291, 1650, 1762, 2067
SfcI CTRYAG 3 cut(s) 552, 1017, 2329
SfuI TTCGAA 2 cut(s) 154, 715
SinI GGWCC 4 cut(s) 1452, 1642, 1923, 2272
SmiMI CAYNNNNRTG 1 cut(s) 1076
SmlI CTYRAG 1 cut(s) 1056
SmoI CTYRAG 1 cut(s) 1056
Sse9I AATT 7 cut(s) 22, 396, 632, 711, 1137, 1707, 2062
SseBI AGGCCT 1 cut(s) 1747
SsiI CCGC 4 cut(s) 223, 1126, 1961, 2013
SspI AATATT 2 cut(s) 1546, 2420
SspMI CTAG 8 cut(s) 8, 494, 1508, 1682, 1743, 1799, 1934, 2319
StuI AGGCCT 1 cut(s) 1747
StyD4I CCNGG 3 cut(s) 1372, 1611, 2405
StyI CCWWGG 3 cut(s) 505, 1149, 1536
TaaI ACNGT 6 cut(s) 76, 566, 780, 1197, 1993, 2346
TaiI ACGT 3 cut(s) 383, 841, 1348
TaqI TCGA 5 cut(s) 154, 288, 715, 1098, 1309
TaqII GACCGA 1 cut(s) 2289
TasI AATT 7 cut(s) 22, 396, 632, 711, 1137, 1707, 2062
TatI WGTACW 5 cut(s) 957, 1407, 1821, 2333, 2380
TfiI GAWTC 3 cut(s) 1039, 1788, 2442
Tru1I TTAA 6 cut(s) 245, 1155, 1412, 1587, 1604, 1893
Tru9I TTAA 6 cut(s) 245, 1155, 1412, 1587, 1604, 1893
TscAI CASTG 5 cut(s) 214, 1026, 1454, 1731, 1844
TseFI GTSAC 5 cut(s) 265, 896, 1061, 2181, 2409
Tsp45I GTSAC 5 cut(s) 265, 896, 1061, 2181, 2409
TspDTI ATGAA 4 cut(s) 309, 623, 1066, 2282
TspRI CASTG 5 cut(s) 214, 1026, 1454, 1731, 1844
VpaK11BI GGWCC 4 cut(s) 1452, 1642, 1923, 2272
XapI RAATTY 1 cut(s) 632
XbaI TCTAGA 2 cut(s) 7, 1933
XceI RCATGY 2 cut(s) 1383, 2387
XcmI CCANNNNNNNNNTGG 2 cut(s) 385, 1446
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 2 cut(s) 1223, 2441
XspI CTAG 8 cut(s) 8, 494, 1508, 1682, 1743, 1799, 1934, 2319
ZrmI AGTACT 2 cut(s) 1409, 2335
Zsp2I ATGCAT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.