Prupe.1G229100_v2.0.a1

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002001
Physical Location & Seq
Reverse (-)
24202670 .. 24211974
9305 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G229100.5

Sequence Viewer

Length: 3909 bp
ATGGAGGGACGAGCAAATGCTGAACCTATTGACCTGGACAAAACAACAGTGACGTTGGAACCTGAGGTGGACAACGCTGGGGGATTATTTGTTCCTGGGAAGGATAGGGTCGTTTTTAGACCTCCAGAGAGGAAATCTCTCTTAGGGTTAGATGTCCTTGCTATTGCAAAACGGGAAGGGTCTAAGGTTGATGGTGGATTCAAGACCCCAAGAGAAAGAGTTGTATCTGTTGTCTCATCAATAGAGGAAGAGGAACAATCAGAGTCTGTAAATACAGATGAAAAAGGGAGTGATGCATCCCCTGCTATACAAAGTCACTCTAGGAGGCGATATCGTGAAATATCTGGGAGTGAGACACCGCGTACAGAAAGTACTGTAACAGAGGAAGGACAAGTTGATTATATGTGCGGGACTCGTCACTCTAGGGAACATCTGCGGGCAGATGTTTTGGCCACACCTAGTGGAAGTTCACACAGTGTTAGGTCTCGGACTCCTAAGTATGACAGGGATGATCGTGGTAGTGAGAGAAGAGATGACAATGGTAGGTATGAAAGGGAGGACCGTGGTAGCGAGAGAAGAGAGTACCAAGATGGTAACAGGAGTGAGAGACAAAGATATGGTAATGGTAAGGATTATTATCGTCGAAGGGAAGGTGGCCGATATGAGCAAGAATATGGTGGAGAATATGGAAGAAAGCAGCGTAGATATGAAGATTCAAAGAGGACACCTGGCAGATCTGACTGGGATGACGGGAGATGGGAATGGGAGGAGTCACCACGACGTGACAGCTACTCTAATACCAGTAGGCGCCATCAACCGTCACCATCACCTATGCTACTTGGGGCCTCACCTGATGCACGACTAGTTTCCCCATGGTTGGGTGGCTACACACCTCATTCTTCTGGTTCTGCTGCTTCTCCCTGGGATCACATCTCCCCCTCTCCTGCCCCAATACGTGCTTCTGGATACTCAGTCAAATCTTCAAGTTCGAAACATGGTGCGAGGTCCCATGAACTTACTTTTTCTTCAGAAAGTTCACAATCATTTGAGGATGCAGAAGCAGATAACACTGATTCGGCTGAAGAACACAAATATGAGATTAGTGAAAGCATGCGTATAGAGATGGAATACAATTCTGACCGTGCATGGTACGATAGAGAAGAAGGAAACACAATGTTTGATACGACTGATGACTCATCCTTATTTTATGGAAATGATGCTTCATATCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAAAGATGGAACTAAGATGTCTCTTGCTCAGAGCAAAAAGTTGTCTCAACGCACTGCTGATAATGCTCAGTGGGAGGATCGTCAACTTTTGAGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAGTTCGACGATGAAGAAGAGCGGAAAGTTATTCTTCTTGTACATGACACAAAGCCTCCTTTCCTGGACGGCCGGGTTGTTTATACTAAGCAAGCAGAGCCCATAATGCCTATAAAGGATCCCACATCAGATATGGCCATAATTTCACGCAAAGGGTCTACTCTGGTTAGGGAAATCCATGAGAAGCAAAGTATGAACAAGTCACGTCAGCGTTTCTGGGAGCTTGCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAGAAATCGGCAGAGCAGATTGACGCAGATACTGCTGCAGTAGGTGAAGATGGTGAAATTGATTTCAAAGAAGATGCCAAGTTTGCACAACATATGAAGAGTGGGGAGGCTGTGAGTGATTTTGCATTGTCAAAAACACTCTCACAGCAACGACAATATTTGCCCATATTTTCTGTGCGGGATGAGTTATTGCAGGTGATTCGAGAAAACCAGGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAGTATCTTCACGAAGATGGGTACACAGTAAACGGTATAGTGGGTTGCACCCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAAGAGATGGAGACGGAGCTTGGTGATAAAGTTGGCTATGCTATTCGTTTTGAGGATGTGACTGGGCCAAATACCGTAATAAAGTACATGACTGATGGAGTACTTCTGCGTGAAACGCTCAGAGATTCTGACCTTGACAAGTACCGTGTAGTTGTGATGGACGAAGCCCATGAAAGGTCACTCAACACGGATGTGCTGTTTGGGATTTTGAAAAAAGTGGTGGCCCAACGTCGTGATTTTAAGCTCATTGTGACATCTGCAACTCTAAATGCTCAGAAATTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATCTTGTACAGCAAAACCCCGTGCGAAGATTATGTTGAAGGTGCAGTGAAGCAGGCCATGACTATTCACATCACCAGCCCTCCAGGTGACATTCTCATCTTCATGACAGGCCAAGATGAGATTGAGGCAGCCTGTTATGCACTTGCTGAGCGTATGGAACAACTTATTTCATCCTCCAAGAAAGGGGTGCCAAAACTCTTGATCCTCCCCATATATTCCCAGTTGCCTGCTGACTTGCAAGCAAAGATATTCCAAAAAGCTGAGGATGGTGCTCGCAAGTGCATTGTAGCAACCAACATCGCTGAGACTTCGTTGACTGTGGATGGAATATTTTACGTTATTGACACTGGGTATGGTAAAATGAAAGTATACAACCCTCGAATGGGTATGGATGCTCTTCAAGTGTTTCCTGTTAGTCGTGCTGCTGCGGATCAGCGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGCTATCGGTTGTACACAGAGAATGCATATCTAAATGAAATGCTGCCCAGTCCTGTGCCAGAGATTCAGAGGACTAACCTTGGTAATGTGGTGTTGTTGCTCAAGAGTCTTAAGGTCGAGAACCTGCTAGATTTCGATTTCATGGACCCACCTCCACAGGACAACATTCTCAATTCTATGTACCAGCTGTGGGTCTTAGGCGCTCTTAACAATGTTGGGGGCTTAACTGATCTGGGCTGGAAAATGGTGGAGTTCCCATTGGACCCCCCACTTGCTAAGATGCTATTGATGGGGGAAGAGCTAGGATGCTTGGATGAGGTTTTGACAATTGTGTCCATGCTTTCGGTGCCGTCCGTATTCTTTAGGCCTAAGGATAGGGCAGAAGAGAGTGATGCTGCACGTGAGAAATTCTCCATTCCAGAATCCGATCACTTGACACTGTATAATGTCTACCAGCAGTGGAAACAACATCAGTATCGTGGGGATTGGTGCGGTGATCATTTTTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGGTCCCAGCTGCTAGAGATCCTGAAGACACTGAAAGTCCCTTTAACATCCTGCTGGCCTGATACAGATAATGTGAGAAAAGCTATTTGCTCTGCGTACTTTCACAATTCTGCAAGATTGAAGGGAGTCGGGGAGTATGTGAATTGCCGGACCGGGATGCCTTGCCATTTACATCCGAGCAGTGCTCTTTATGGTATGGGGTGCACTCCGGATTATCTCGTTTATCATGAATTGATTTTGACGGCAAAGGAGTATATGCAGTGTGCCACAGCAGTGGAACCACAGTGGTTGGCTGAGTTGGGGCCTATGTTCTTCTCTGTAAAGGATTCGGATACTTCAATGTTGGAGCACAAGAAGAGACAAAAGGAGGAGAAAACAGCCATGGAGGAGGAAATGGAGAACCTGAGGAAGACTCAAGCAGAGGAGGAGAAAGAAAACAAACGGAAGGAGAAGGAAAAGAGATCCAAGCAGCAGCAGCAAGTCTCAACGCCGGGTTTGCACAAGGGCTCTTCCACATATTTGAGGCCAAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1303

Amino Acids

147.04

Weight (kDa)

5.86

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1834
AasI GACNNNNNNGTC 1 cut(s) 3154
Acc36I ACCTGC 3 cut(s) 1610, 1834, 2955
AccB1I GGYRCC 3 cut(s) 807, 2539, 3169
AccBSI CCGCTC 1 cut(s) 1414
AccI GTMKAC 3 cut(s) 1550, 2721, 3273
AccII CGCG 1 cut(s) 361
AccIII TCCGGA 1 cut(s) 3571
AciI CCGC 7 cut(s) 359, 408, 436, 1414, 1828, 2780, 3315
AclWI GGATC 8 cut(s) 933, 1350, 1505, 1518, 2548, 2790, 3376, 3819
AcoI YGGCCR 4 cut(s) 450, 655, 1462, 1527
AcsI RAATTY 3 cut(s) 2288, 2296, 3230
AcuI CTGAAG 3 cut(s) 1011, 1101, 3407
AcvI CACGTG 1 cut(s) 3224
AcyI GRCGYC 1 cut(s) 808
AdeI CACNNNGTG 3 cut(s) 461, 476, 782
AfiI CCNNNNNNNGG 8 cut(s) 877, 878, 1971, 2184, 2535, 2734, 2735, 3013
AflII CTTAAG 1 cut(s) 2933
AhlI ACTAGT 1 cut(s) 862
AjiI CACGTC 2 cut(s) 782, 1598
AjnI CCWGG 9 cut(s) 33, 94, 727, 920, 1455, 1860, 2329, 2434, 2815
AjuI GAANNNNNNNTTGG 2 cut(s) 943, 975
AleI CACNNNNGTG 2 cut(s) 2201, 3635
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
AluBI AGCT 9 cut(s) 789, 1615, 2029, 2254, 2612, 3010, 3124, 3373, 3446
AluI AGCT 9 cut(s) 789, 1615, 2029, 2254, 2612, 3010, 3124, 3373, 3446
Alw21I GWGCWC 4 cut(s) 2626, 3550, 3569, 3714
Alw44I GTGCAC 1 cut(s) 3565
AlwI GGATC 8 cut(s) 933, 1350, 1505, 1518, 2548, 2790, 3376, 3819
AlwNI CAGNNNCTG 2 cut(s) 266, 1682
Aor13HI TCCGGA 1 cut(s) 3571
ApaLI GTGCAC 1 cut(s) 3565
ApoI RAATTY 3 cut(s) 2288, 2296, 3230
AspLEI GCGC 2 cut(s) 810, 3026
AsuC2I CCSGG 3 cut(s) 1466, 3517, 3855
AsuII TTCGAA 2 cut(s) 989, 1264
AvaII GGWCC 6 cut(s) 559, 1005, 2968, 3085, 3366, 3513
AxyI CCTNAGG 3 cut(s) 63, 3192, 3767
BaeGI GKGCMC 1 cut(s) 3569
BaeI ACNNNNGTAYC 4 cut(s) 1368, 1401, 3281, 3314
BalI TGGCCA 2 cut(s) 452, 1529
BamHI GGATCC 1 cut(s) 1510
BanI GGYRCC 3 cut(s) 807, 2539, 3169
BanII GRGCYC 2 cut(s) 1494, 3872
BbrPI CACGTG 1 cut(s) 3224
BbsI GAAGAC 2 cut(s) 3395, 3779
Bbv12I GWGCWC 4 cut(s) 2626, 3550, 3569, 3714
BbvCI CCTCAGC 1 cut(s) 2613
BceAI ACGGC 3 cut(s) 1477, 3157, 3621
BcgI CGANNNNNNTGC 2 cut(s) 1832, 1866
BciT130I CCWGG 9 cut(s) 35, 96, 729, 922, 1457, 1862, 2331, 2436, 2817
BciVI GTATCC 2 cut(s) 959, 3688
BclI TGATCA 1 cut(s) 3319
BcnI CCSGG 3 cut(s) 1466, 3517, 3855
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 8 cut(s) 321, 423, 459, 863, 2951, 3125, 3354, 3377
BfmI CTRYAG 1 cut(s) 1686
BfoI RGCGCY 2 cut(s) 811, 3027
BfrI CTTAAG 1 cut(s) 2933
BfuAI ACCTGC 3 cut(s) 1610, 1834, 2955
BfuI GTATCC 2 cut(s) 959, 3688
BglII AGATCT 2 cut(s) 734, 1358
BlpI GCTNAGC 1 cut(s) 2499
BmcAI AGTACT 2 cut(s) 373, 2112
Bme18I GGWCC 6 cut(s) 559, 1005, 2968, 3085, 3366, 3513
BmgBI CACGTC 2 cut(s) 782, 1598
BmrI ACTGGG 6 cut(s) 751, 2082, 2566, 2709, 2820, 2865
BmuI ACTGGG 6 cut(s) 751, 2082, 2566, 2709, 2820, 2865
BpiI GAAGAC 2 cut(s) 3395, 3779
BplI GAGNNNNNCTC 8 cut(s) 121, 153, 3218, 3250, 3532, 3564, 3760, 3792
BpmI CTGGAG 2 cut(s) 108, 2418
Bpu10I CCTNAGC 1 cut(s) 2613
Bpu1102I GCTNAGC 1 cut(s) 2499
Bpu14I TTCGAA 2 cut(s) 989, 1264
BpuEI CTTGAG 2 cut(s) 2909, 3762
BpuMI CCSGG 3 cut(s) 1466, 3517, 3855
BsaAI YACGTR 2 cut(s) 956, 3224
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 808
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 9 cut(s) 95, 562, 872, 920, 921, 1970, 2330, 2902, 3744
BsaWI WCCGGW 1 cut(s) 3571
BsaXI ACNNNNNCTCC 4 cut(s) 1432, 1462, 2416, 2446
Bsc4I CCNNNNNNNGG 8 cut(s) 877, 878, 1971, 2184, 2535, 2734, 2735, 3013
Bse1I ACTGG 8 cut(s) 746, 801, 1891, 2077, 2572, 2704, 2815, 2871
Bse21I CCTNAGG 3 cut(s) 63, 3192, 3767
Bse8I GATNNNNATC 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 3571
BseBI CCWGG 9 cut(s) 35, 96, 729, 922, 1457, 1862, 2331, 2436, 2817
BseDI CCNNGG 9 cut(s) 95, 562, 872, 920, 921, 1970, 2330, 2902, 3744
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 8 cut(s) 877, 878, 1971, 2184, 2535, 2734, 2735, 3013
BseNI ACTGG 8 cut(s) 746, 801, 1891, 2077, 2572, 2704, 2815, 2871
BseRI GAGGAG 5 cut(s) 782, 3746, 3764, 3800, 3803
BseSI GKGCMC 1 cut(s) 3569
BseX3I CGGCCG 1 cut(s) 1462
BseYI CCCAGC 3 cut(s) 77, 2792, 3369
BsgI GTGCAG 3 cut(s) 1406, 2415, 3204
Bsh1236I CGCG 1 cut(s) 361
Bsh1285I CGRYCG 1 cut(s) 1465
BshNI GGYRCC 3 cut(s) 807, 2539, 3169
BsiEI CGRYCG 1 cut(s) 1465
BsiHKAI GWGCWC 4 cut(s) 2626, 3550, 3569, 3714
BsiSI CCGG 5 cut(s) 1465, 3511, 3516, 3572, 3854
BslFI GGGAC 5 cut(s) 21, 424, 991, 3352, 3386
BslI CCNNNNNNNGG 8 cut(s) 877, 878, 1971, 2184, 2535, 2734, 2735, 3013
BsmBI CGTCTC 1 cut(s) 2015
BsmFI GGGAC 5 cut(s) 21, 424, 991, 3352, 3386
BsmI GAATGC 1 cut(s) 2851
Bso31I GGTCTC 1 cut(s) 489
Bsp119I TTCGAA 2 cut(s) 989, 1264
Bsp1286I GDGCHC 6 cut(s) 1494, 2626, 3550, 3569, 3714, 3872
Bsp13I TCCGGA 1 cut(s) 3571
Bsp1407I TGTACA 3 cut(s) 1432, 2358, 2835
Bsp1720I GCTNAGC 1 cut(s) 2499
Bsp19I CCATGG 2 cut(s) 872, 3744
BspACI CCGC 7 cut(s) 359, 408, 436, 1414, 1828, 2780, 3315
BspEI TCCGGA 1 cut(s) 3571
BspFNI CGCG 1 cut(s) 361
BspHI TCATGA 2 cut(s) 2454, 3589
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 3 cut(s) 1610, 1834, 2955
BspPI GGATC 8 cut(s) 933, 1350, 1505, 1518, 2548, 2790, 3376, 3819
BspQI GCTCTTC 4 cut(s) 1404, 2754, 3114, 3877
BspT104I TTCGAA 2 cut(s) 989, 1264
BspT107I GGYRCC 3 cut(s) 807, 2539, 3169
BspTI CTTAAG 1 cut(s) 2933
BspTNI GGTCTC 1 cut(s) 489
BsrBI CCGCTC 1 cut(s) 1414
BsrGI TGTACA 3 cut(s) 1432, 2358, 2835
BsrI ACTGG 8 cut(s) 746, 801, 1891, 2077, 2572, 2704, 2815, 2871
BssECI CCNNGG 9 cut(s) 95, 562, 872, 920, 921, 1970, 2330, 2902, 3744
BssNAI GTATAC 1 cut(s) 2722
BssNI GRCGYC 1 cut(s) 808
BssT1I CCWWGG 4 cut(s) 872, 1970, 2902, 3744
Bst1107I GTATAC 1 cut(s) 2722
Bst2UI CCWGG 9 cut(s) 35, 96, 729, 922, 1457, 1862, 2331, 2436, 2817
BstACI GRCGYC 1 cut(s) 808
BstAFI CTTAAG 1 cut(s) 2933
BstAPI GCANNNNNTGC 2 cut(s) 302, 1682
BstAUI TGTACA 3 cut(s) 1432, 2358, 2835
BstBAI YACGTR 2 cut(s) 956, 3224
BstBI TTCGAA 2 cut(s) 989, 1264
BstDSI CCRYGG 3 cut(s) 562, 872, 3744
BstFNI CGCG 1 cut(s) 361
BstH2I RGCGCY 2 cut(s) 811, 3027
BstHHI GCGC 2 cut(s) 810, 3026
BstMCI CGRYCG 1 cut(s) 1465
BstNI CCWGG 9 cut(s) 35, 96, 729, 922, 1457, 1862, 2331, 2436, 2817
BstNSI RCATGY 3 cut(s) 1114, 2826, 3335
BstSFI CTRYAG 1 cut(s) 1686
BstSLI GKGCMC 1 cut(s) 3569
BstUI CGCG 1 cut(s) 361
BstV2I GAAGAC 2 cut(s) 3395, 3779
BstX2I RGATCY 5 cut(s) 734, 1358, 1510, 3381, 3824
BstXI CCANNNNNNTGG 1 cut(s) 3637
BstYI RGATCY 5 cut(s) 734, 1358, 1510, 3381, 3824
BstZ17I GTATAC 1 cut(s) 2722
BstZI CGGCCG 1 cut(s) 1462
Bsu36I CCTNAGG 3 cut(s) 63, 3192, 3767
BsuI GTATCC 2 cut(s) 959, 3688
BtgI CCRYGG 3 cut(s) 562, 872, 3744
BtgZI GCGATG 1 cut(s) 2635
BtrI CACGTC 2 cut(s) 782, 1598
BtsI GCAGTG 6 cut(s) 1317, 2403, 3287, 3550, 3629, 3642
BveI ACCTGC 3 cut(s) 1610, 1834, 2955
CaiI CAGNNNCTG 2 cut(s) 266, 1682
CciI TCATGA 2 cut(s) 2454, 3589
CfoI GCGC 2 cut(s) 810, 3026
CpoI CGGWCCG 1 cut(s) 3513
CseI GACGC 1 cut(s) 1682
CsiI ACCWGGT 1 cut(s) 1860
CspCI CAANNNNNGTGG 4 cut(s) 1959, 1994, 3633, 3668
CspI CGGWCCG 1 cut(s) 3513
DinI GGCGCC 1 cut(s) 809
DraIII CACNNNGTG 3 cut(s) 461, 476, 782
DrdI GACNNNNNNGTC 1 cut(s) 3154
DseDI GACNNNNNNGTC 1 cut(s) 3154
EaeI YGGCCR 4 cut(s) 450, 655, 1462, 1527
EagI CGGCCG 1 cut(s) 1462
EclXI CGGCCG 1 cut(s) 1462
Eco130I CCWWGG 4 cut(s) 872, 1970, 2902, 3744
Eco147I AGGCCT 1 cut(s) 3190
Eco24I GRGCYC 2 cut(s) 1494, 3872
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 332
Eco47I GGWCC 6 cut(s) 559, 1005, 2968, 3085, 3366, 3513
Eco52I CGGCCG 1 cut(s) 1462
Eco57I CTGAAG 3 cut(s) 1011, 1101, 3407
Eco72I CACGTG 1 cut(s) 3224
Eco81I CCTNAGG 3 cut(s) 63, 3192, 3767
EcoO109I RGGNCCY 4 cut(s) 843, 1005, 3366, 3665
EcoRII CCWGG 9 cut(s) 33, 94, 727, 920, 1455, 1860, 2329, 2434, 2815
EcoRV GATATC 1 cut(s) 332
EcoT14I CCWWGG 4 cut(s) 872, 1970, 2902, 3744
EcoT22I ATGCAT 2 cut(s) 298, 2851
EcoT38I GRGCYC 2 cut(s) 1494, 3872
EgeI GGCGCC 1 cut(s) 809
EheI GGCGCC 1 cut(s) 809
ErhI CCWWGG 4 cut(s) 872, 1970, 2902, 3744
Esp3I CGTCTC 1 cut(s) 2015
FalI AAGNNNNNCTT 2 cut(s) 1410, 1442
FaqI GGGAC 5 cut(s) 21, 424, 991, 3352, 3386
FauI CCCGC 3 cut(s) 401, 429, 1821
FauNDI CATATG 1 cut(s) 1743
FbaI TGATCA 1 cut(s) 3319
FblI GTMKAC 3 cut(s) 1550, 2721, 3273
FriOI GRGCYC 2 cut(s) 1494, 3872
FspBI CTAG 8 cut(s) 321, 423, 459, 863, 2951, 3125, 3354, 3377
GlaI GCGC 2 cut(s) 809, 3025
GsaI CCCAGC 3 cut(s) 81, 2796, 3373
GsuI CTGGAG 2 cut(s) 108, 2418
HaeII RGCGCY 2 cut(s) 811, 3027
HapII CCGG 5 cut(s) 1465, 3511, 3516, 3572, 3854
HgaI GACGC 1 cut(s) 1682
HhaI GCGC 2 cut(s) 810, 3026
Hin1I GRCGYC 1 cut(s) 808
Hin6I GCGC 2 cut(s) 808, 3024
HinP1I GCGC 2 cut(s) 808, 3024
HincII GTYRAC 2 cut(s) 1349, 2667
HindII GTYRAC 2 cut(s) 1349, 2667
HpaII CCGG 5 cut(s) 1465, 3511, 3516, 3572, 3854
Hpy99I CGWCG 4 cut(s) 645, 783, 1403, 2244
HpyCH4IV ACGT 7 cut(s) 53, 781, 955, 1597, 2239, 2688, 3223
HpySE526I ACGT 7 cut(s) 53, 781, 955, 1597, 2239, 2688, 3223
Hsp92I GRCGYC 1 cut(s) 808
HspAI GCGC 2 cut(s) 808, 3024
KasI GGCGCC 1 cut(s) 807
Kpn2I TCCGGA 1 cut(s) 3571
Ksp22I TGATCA 1 cut(s) 3319
LguI GCTCTTC 4 cut(s) 1404, 2754, 3114, 3877
LmnI GCTCC 3 cut(s) 1612, 2026, 3709
MabI ACCWGGT 1 cut(s) 1860
MaeI CTAG 8 cut(s) 321, 423, 459, 863, 2951, 3125, 3354, 3377
MaeII ACGT 7 cut(s) 53, 781, 955, 1597, 2239, 2688, 3223
MbiI CCGCTC 1 cut(s) 1414
MfeI CAATTG 1 cut(s) 3150
MflI RGATCY 5 cut(s) 734, 1358, 1510, 3381, 3824
MhlI GDGCHC 6 cut(s) 1494, 2626, 3550, 3569, 3714, 3872
MlsI TGGCCA 2 cut(s) 452, 1529
MluNI TGGCCA 2 cut(s) 452, 1529
Mly113I GGCGCC 1 cut(s) 808
MlyI GAGTC 8 cut(s) 272, 406, 484, 779, 1187, 2938, 3498, 3769
MmeI TCCRAC 3 cut(s) 36, 1858, 3687
Mox20I TGGCCA 2 cut(s) 452, 1529
Mph1103I ATGCAT 2 cut(s) 298, 2851
MroI TCCGGA 1 cut(s) 3571
MscI TGGCCA 2 cut(s) 452, 1529
MseI TTAA 7 cut(s) 2250, 2349, 2934, 3030, 3047, 3336, 3407
MslI CAYNNNNRTG 5 cut(s) 1092, 1929, 2201, 2453, 3635
Msp20I TGGCCA 2 cut(s) 452, 1529
MspA1I CMGCKG 2 cut(s) 3010, 3373
MspCI CTTAAG 1 cut(s) 2933
MspI CCGG 5 cut(s) 1465, 3511, 3516, 3572, 3854
MunI CAATTG 1 cut(s) 3150
Mva1269I GAATGC 1 cut(s) 2851
MvaI CCWGG 9 cut(s) 35, 96, 729, 922, 1457, 1862, 2331, 2436, 2817
MvnI CGCG 1 cut(s) 361
NarI GGCGCC 1 cut(s) 808
NciI CCSGG 3 cut(s) 1466, 3517, 3855
NcoI CCATGG 2 cut(s) 872, 3744
NdeI CATATG 1 cut(s) 1743
NsiI ATGCAT 2 cut(s) 298, 2851
NspI RCATGY 3 cut(s) 1114, 2826, 3335
NspV TTCGAA 2 cut(s) 989, 1264
OliI CACNNNNGTG 2 cut(s) 2201, 3635
PaeI GCATGC 1 cut(s) 1114
PagI TCATGA 2 cut(s) 2454, 3589
PaqCI CACCTGC 1 cut(s) 1834
PasI CCCWGGG 1 cut(s) 921
PceI AGGCCT 1 cut(s) 3190
PciSI GCTCTTC 4 cut(s) 1404, 2754, 3114, 3877
PctI GAATGC 1 cut(s) 2851
PfeI GAWTC 8 cut(s) 198, 713, 1073, 1849, 2135, 2887, 3245, 3689
PfoI TCCNGGA 1 cut(s) 1455
PleI GAGTC 8 cut(s) 271, 406, 484, 778, 1187, 2937, 3497, 3769
PluTI GGCGCC 1 cut(s) 811
PmaCI CACGTG 1 cut(s) 3224
PmlI CACGTG 1 cut(s) 3224
PpsI GAGTC 8 cut(s) 271, 406, 484, 778, 1187, 2937, 3497, 3769
Ppu21I YACGTR 2 cut(s) 956, 3224
PpuMI RGGWCCY 2 cut(s) 1005, 3366
Psp5II RGGWCCY 2 cut(s) 1005, 3366
Psp6I CCWGG 9 cut(s) 33, 94, 727, 920, 1455, 1860, 2329, 2434, 2815
PspCI CACGTG 1 cut(s) 3224
PspFI CCCAGC 3 cut(s) 77, 2792, 3369
PspGI CCWGG 9 cut(s) 33, 94, 727, 920, 1455, 1860, 2329, 2434, 2815
PspPPI RGGWCCY 2 cut(s) 1005, 3366
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 2 cut(s) 266, 1682
PsuI RGATCY 5 cut(s) 734, 1358, 1510, 3381, 3824
PvuII CAGCTG 2 cut(s) 3010, 3373
RseI CAYNNNNRTG 5 cut(s) 1092, 1929, 2201, 2453, 3635
Rsr2I CGGWCCG 1 cut(s) 3513
RsrII CGGWCCG 1 cut(s) 3513
SapI GCTCTTC 4 cut(s) 1404, 2754, 3114, 3877
SaqAI TTAA 7 cut(s) 2250, 2349, 2934, 3030, 3047, 3336, 3407
ScaI AGTACT 2 cut(s) 373, 2112
SchI GAGTC 8 cut(s) 272, 406, 484, 779, 1187, 2938, 3498, 3769
SduI GDGCHC 6 cut(s) 1494, 2626, 3550, 3569, 3714, 3872
SexAI ACCWGGT 1 cut(s) 1860
SfcI CTRYAG 1 cut(s) 1686
SfoI GGCGCC 1 cut(s) 809
SfuI TTCGAA 2 cut(s) 989, 1264
SinI GGWCC 6 cut(s) 559, 1005, 2968, 3085, 3366, 3513
SmiMI CAYNNNNRTG 5 cut(s) 1092, 1929, 2201, 2453, 3635
SmlI CTYRAG 3 cut(s) 2924, 2933, 3777
SmoI CTYRAG 3 cut(s) 2924, 2933, 3777
SpeI ACTAGT 1 cut(s) 862
SphI GCATGC 1 cut(s) 1114
SseBI AGGCCT 1 cut(s) 3190
SsiI CCGC 7 cut(s) 359, 408, 436, 1414, 1828, 2780, 3315
SspDI GGCGCC 1 cut(s) 807
SspI AATATT 2 cut(s) 1808, 2682
SspMI CTAG 8 cut(s) 321, 423, 459, 863, 2951, 3125, 3354, 3377
StuI AGGCCT 1 cut(s) 3190
StyI CCWWGG 4 cut(s) 872, 1970, 2902, 3744
TaiI ACGT 7 cut(s) 56, 784, 958, 1600, 2242, 2691, 3226
TaqI TCGA 8 cut(s) 643, 989, 1264, 1398, 1852, 2731, 2940, 2958
TatI WGTACW 6 cut(s) 371, 1432, 2094, 2110, 2358, 2835
TfiI GAWTC 8 cut(s) 198, 713, 1073, 1849, 2135, 2887, 3245, 3689
Tru1I TTAA 7 cut(s) 2250, 2349, 2934, 3030, 3047, 3336, 3407
Tru9I TTAA 7 cut(s) 2250, 2349, 2934, 3030, 3047, 3336, 3407
TspGWI ACGGA 4 cut(s) 2039, 2213, 3166, 3820
Vha464I CTTAAG 1 cut(s) 2933
VneI GTGCAC 1 cut(s) 3565
VpaK11BI GGWCC 6 cut(s) 559, 1005, 2968, 3085, 3366, 3513
XapI RAATTY 3 cut(s) 2288, 2296, 3230
XceI RCATGY 3 cut(s) 1114, 2826, 3335
XcmI CCANNNNNNNNNTGG 1 cut(s) 1522
XmiI GTMKAC 3 cut(s) 1550, 2721, 3273
XspI CTAG 8 cut(s) 321, 423, 459, 863, 2951, 3125, 3354, 3377
ZrmI AGTACT 2 cut(s) 373, 2112
Zsp2I ATGCAT 2 cut(s) 298, 2851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.