Rh4AG307100

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002001
Physical Location & Seq
Reverse (-)
61893316 .. 61893642
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG307100.1

Sequence Viewer

Length: 327 bp
ATGGGATGCACTCCAGACTATGTCGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTAGGGCCCATATTCTTCTCTGTGAAGGATTCAGATACATCGCTATTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACATTTATGGGGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAGGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAGGCCAAAGAAACTTAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

108

Amino Acids

12.66

Weight (kDa)

7.75

Isoelectric Point (pI)

67.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 5 - 37 1.2e-09 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 98
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 1 cut(s) 59
AgsI TTSAA 1 cut(s) 291
AjnI CCWGG 1 cut(s) 295
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 1 cut(s) 321
AluI AGCT 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 1 cut(s) 261
AoxI GGCC 2 cut(s) 107, 305
ApaI GGGCCC 1 cut(s) 111
ApeKI GCWGC 3 cut(s) 274, 277, 280
ApoI RAATTY 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 107, 108
BaeGI GKGCMC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 286, 289, 292
BccI CCATC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 157
BfmI CTRYAG 1 cut(s) 75
BisI GCNGC 3 cut(s) 275, 278, 281
BlsI GCNGC 3 cut(s) 276, 279, 282
Bme1390I CCNGG 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 107, 108
BmiI GGNNCC 2 cut(s) 84, 109
BmrFI CCNGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpuEI CTTGAG 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 11
BseMII CTCAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 3 cut(s) 286, 289, 292
BshFI GGCC 2 cut(s) 109, 307
BsmAI GTCTC 1 cut(s) 157
BsnI GGCC 2 cut(s) 109, 307
Bsp120I GGGCCC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 266
BspACI CCGC 1 cut(s) 98
BspANI GGCC 2 cut(s) 109, 307
BspCNI CTCAG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 84, 109
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 1 cut(s) 261
BssMI GATC 1 cut(s) 266
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 90
Bst6I CTCTTC 2 cut(s) 155, 168
BstDEI CTNAG 2 cut(s) 209, 317
BstF5I GGATG 1 cut(s) 11
BstKTI GATC 1 cut(s) 269
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 266
BstMWI GCNNNNNNNGC 2 cut(s) 151, 280
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 75
BstSLI GKGCMC 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 286, 289, 292
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 2 cut(s) 109, 307
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 11
BtsI GCAGTG 3 cut(s) 71, 72, 84
BtsIMutI CAGTG 4 cut(s) 71, 72, 84, 95
CciI TCATGA 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 107, 108
Csp6I GTAC 1 cut(s) 58
CspCI CAANNNNNGTGG 2 cut(s) 75, 110
CviAII CATG 2 cut(s) 32, 61
CviJI RGCY 5 cut(s) 85, 109, 218, 307, 321
CviKI_1 RGCY 5 cut(s) 85, 109, 218, 307, 321
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 209, 317
DpnI GATC 1 cut(s) 268
DpnII GATC 1 cut(s) 266
Eam1104I CTCTTC 2 cut(s) 155, 168
EarI CTCTTC 2 cut(s) 155, 168
EciI GGCGGA 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 2 cut(s) 35, 64
FaiI YATR 6 cut(s) 21, 33, 62, 113, 156, 188
FatI CATG 2 cut(s) 31, 60
Fnu4HI GCNGC 3 cut(s) 275, 278, 281
FokI GGATG 1 cut(s) 18
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 275, 278, 281
GluI GCNGC 3 cut(s) 275, 278, 281
HaeIII GGCC 2 cut(s) 109, 307
Hin1II CATG 2 cut(s) 35, 64
HinfI GANTC 2 cut(s) 131, 205
Hpy188I TCNGA 2 cut(s) 136, 210
Hpy188III TCNNGA 2 cut(s) 14, 32
HpyAV CCTTC 2 cut(s) 46, 121
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 9, 64, 77
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 280
HpyF3I CTNAG 2 cut(s) 209, 317
Hsp92II CATG 2 cut(s) 35, 64
Kzo9I GATC 1 cut(s) 266
LmnI GCTCC 2 cut(s) 82, 151
LpnPI CCDG 3 cut(s) 27, 282, 309
Lsp1109I GCAGC 3 cut(s) 286, 289, 292
MalI GATC 1 cut(s) 268
MboI GATC 1 cut(s) 266
MboII GAAGA 4 cut(s) 109, 172, 185, 190
MflI RGATCY 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 1 cut(s) 285
MnlI CCTC 3 cut(s) 220, 243, 297
MslI CAYNNNNRTG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 151, 280
NdeII GATC 1 cut(s) 266
NlaIII CATG 2 cut(s) 35, 64
NlaIV GGNNCC 2 cut(s) 84, 109
NspI RCATGY 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 77
PagI TCATGA 1 cut(s) 31
PfeI GAWTC 2 cut(s) 131, 205
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 3 cut(s) 276, 279, 282
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 84, 109
PspOMI GGGCCC 1 cut(s) 107
PspPI GGNCC 2 cut(s) 107, 108
PstI CTGCAG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 266
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 3 cut(s) 275, 278, 281
Sau3AI GATC 1 cut(s) 266
Sau96I GGNCC 2 cut(s) 107, 108
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 111
SetI ASST 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 75
SgeI CNNG 9 cut(s) 26, 44, 73, 206, 233, 256, 283, 308, 309
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 1 cut(s) 285
SsiI CCGC 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 90
TasI AATT 1 cut(s) 285
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 2 cut(s) 131, 205
TscAI CASTG 4 cut(s) 71, 79, 84, 95
TseI GCWGC 3 cut(s) 274, 277, 280
TspDTI ATGAA 1 cut(s) 48
TspRI CASTG 4 cut(s) 71, 79, 84, 95
Tth111I GACNNNGTC 1 cut(s) 20
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.