Rh5BG158600

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002001
Physical Location & Seq
Forward (+)
16163805 .. 16168030
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG158600.1

Sequence Viewer

Length: 2802 bp
ATGCGTTTAGAGATGGAATATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCACAGCGGTTGATACGAGTGATAGTTCGTCTTTGTTTTATGGGGATGATGCTTTTTGTCGAAAGAAGGAAACGGAGTTGGCGAAGAGATTGGTTCGGAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAGGTTGTCTCAGCGCAGTGCGGATAATGCTCAGTGGGAGGATCGTCAGCTTCTGCGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAACAAAGTTATGCTTCTTGTTCATGATACAAGGCCTCCTTTCCTAGATGGGAGAGTTGTTTTTACCAAGCAAGCGGAGCCTGTTATGCCAGTAAAGGATCCCACGTCTGATATGGCCATAATTTCACGCAAAGGGTCTGCTCTAGTTAGGGAAATCCATCAAAAGCAAAGTTCGAATAAATCACGTCAACGTTTTTGGGAGCTTGCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAGAAAACAGCAGAGCATATTGATGCAGATACTGCTGTAGTAGGTAAAGACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCTCATCATATGAAGAATGGGGAGGCTGTGAGTGAATTCACGAAGTCAAAAACCATTTCTGAACAACGCCAGTATCTGCCTATATATTCTGTGCGGGATGAGTTACTGCAGAAAATTCGAGAAAATCAGGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGTTACACTGTGAACGGTATAGTCGGTTGCACCCAGCCAAGGCGTGTGGCAGCCATGAGTGTTGCTAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGAGAAAGTTGGCTATGCTATTCGTTTTGAGGATTTGACGGGGCCAAAAACTGTAATAAAGTACATGACCGATGGAGTACTTATGCGTGAGACACTTAGAGATTCTGACCTTGACAAGTACCGCGTAGTTGTAATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATTTTGAAAAAAGTCATCGCCCAGCGTCGTGATTTTAAGCTCATTGTGACATCTGCTACTCTTAATGCTCAGAAATTTGCAGATTTCTTTGGAGGTGCACCTATTTTTCCCATTCCTGGAAGAACATTTCCTGTACATAAGAAGTACTTGGATACCCCATGTGAAGATTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTACCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTTGCAGCGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGAGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTATACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGTTGTACACAGAGAGTGCCTACCTAAATGAAATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTCAAGTCTCTGAAAGTTGATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCAGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGTGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAAAATGGTAGAGTTCCCATTGGACCCTCCACTTGCGAAGATGTTGTTGATGGGTGAAGAGTTAGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCAGAGTCCGATCACCTGACATTCTATAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTACAAAAGGCTAGAGAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACATTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATGGGCTGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACAGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGAGAAACTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAGCAAGCAGAGGGAGAGGGAAAAGAGGTCTAAACAGCAGCAGCAGCAAGTCTCATTGGCGGGTTTGCACCAGGGCTCTTCTGCATATCTGAGGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

933

Amino Acids

105.52

Weight (kDa)

6.27

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 241 - 393 3.8e-09 DEAD/DEAH box helicase
AAA_22 PF13401 249 - 370 3.1e-06 AAA domain
Helicase_C PF00271 438 - 568 5.3e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 633 - 658 5.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 659 - 718 1.7e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 776 - 852 4.5e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 1611
AccII CGCG 2 cut(s) 1047, 1393
AciI CCGC 7 cut(s) 65, 212, 377, 718, 1045, 2534, 2747
AclI AACGTT 1 cut(s) 493
AclWI GGATC 4 cut(s) 240, 395, 408, 1771
AcoI YGGCCR 3 cut(s) 417, 1351, 1758
AcsI RAATTY 7 cut(s) 659, 738, 1178, 1305, 2031, 2120, 2289
AcuI CTGAAG 1 cut(s) 2297
AfiI CCNNNNNNNGG 8 cut(s) 861, 1074, 1220, 1624, 1625, 1903, 1950, 2088
AflIII ACRYGT 1 cut(s) 1712
AgsI TTSAA 7 cut(s) 607, 1111, 1280, 1367, 1643, 2287, 2357
AjiI CACGTC 2 cut(s) 408, 488
AjnI CCWGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
Alw21I GWGCWC 3 cut(s) 1204, 1918, 2440
Alw26I GTCTC 7 cut(s) 204, 905, 1007, 1541, 1824, 2605, 2743
Alw44I GTGCAC 1 cut(s) 1200
AlwI GGATC 4 cut(s) 240, 395, 408, 1771
AlwNI CAGNNNCTG 4 cut(s) 244, 572, 700, 2491
Ama87I CYCGRG 2 cut(s) 2112, 2428
ApaLI GTGCAC 1 cut(s) 1200
ApoI RAATTY 7 cut(s) 659, 738, 1178, 1305, 2031, 2120, 2289
Asp700I GAANNNNTTC 2 cut(s) 1878, 2487
AspA2I CCTAGG 1 cut(s) 1619
AspLEI GCGC 2 cut(s) 207, 1393
AspS9I GGNCC 8 cut(s) 965, 1703, 1858, 1975, 2079, 2256, 2555, 2781
AsuHPI GGTGA 6 cut(s) 536, 605, 935, 1340, 2018, 2135
AsuII TTCGAA 1 cut(s) 476
AvaI CYCGRG 2 cut(s) 2112, 2428
AvaII GGWCC 4 cut(s) 1858, 1975, 2256, 2555
AvrII CCTAGG 1 cut(s) 1619
AxyI CCTNAGG 1 cut(s) 1945
BaeGI GKGCMC 1 cut(s) 1204
BalI TGGCCA 2 cut(s) 419, 1760
BamHI GGATCC 1 cut(s) 400
BanII GRGCYC 2 cut(s) 1516, 2765
BbsI GAAGAC 1 cut(s) 2285
Bbv12I GWGCWC 3 cut(s) 1204, 1918, 2440
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
BciVI GTATCC 3 cut(s) 1249, 2056, 2578
BcoDI GTCTC 7 cut(s) 204, 905, 1007, 1541, 1824, 2605, 2743
BfaI CTAG 6 cut(s) 347, 446, 1620, 1653, 2244, 2267
BfmI CTRYAG 3 cut(s) 576, 731, 1284
BfuAI ACCTGC 1 cut(s) 500
BfuI GTATCC 3 cut(s) 1249, 2056, 2578
BlnI CCTAGG 1 cut(s) 1619
BmcAI AGTACT 2 cut(s) 1002, 1250
Bme1390I CCNGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
Bme18I GGWCC 4 cut(s) 1858, 1975, 2256, 2555
BmeT110I CYCGRG 2 cut(s) 2112, 2428
BmgBI CACGTC 2 cut(s) 408, 488
BmgT120I GGNCC 8 cut(s) 965, 1703, 1858, 1975, 2079, 2256, 2555, 2781
BmrFI CCNGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
BmrI ACTGGG 2 cut(s) 1710, 2415
BmsI GCATC 7 cut(s) 97, 553, 604, 1624, 2009, 2095, 2400
BmuI ACTGGG 2 cut(s) 1710, 2415
BpiI GAAGAC 1 cut(s) 2285
BplI GAGNNNNNCTC 6 cut(s) 166, 198, 2422, 2454, 2650, 2682
BpmI CTGGAG 1 cut(s) 1308
Bpu14I TTCGAA 1 cut(s) 476
BpuEI CTTGAG 2 cut(s) 1799, 2652
BsaAI YACGTR 1 cut(s) 1801
BsaJI CCNNGG 5 cut(s) 860, 1619, 1792, 1869, 2758
BsaXI ACNNNNNCTCC 6 cut(s) 322, 352, 1306, 1336, 2522, 2552
Bsc4I CCNNNNNNNGG 8 cut(s) 861, 1074, 1220, 1624, 1625, 1903, 1950, 2088
Bse118I RCCGGY 1 cut(s) 1349
Bse1I ACTGG 8 cut(s) 392, 694, 781, 1594, 1705, 1761, 2204, 2410
Bse21I CCTNAGG 1 cut(s) 1945
BseBI CCWGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
BseDI CCNNGG 5 cut(s) 860, 1619, 1792, 1869, 2758
BseGI GGATG 7 cut(s) 109, 727, 1066, 1629, 1639, 2024, 2415
BseLI CCNNNNNNNGG 8 cut(s) 861, 1074, 1220, 1624, 1625, 1903, 1950, 2088
BseNI ACTGG 8 cut(s) 392, 694, 781, 1594, 1705, 1761, 2204, 2410
BseRI GAGGAG 5 cut(s) 1373, 1402, 1405, 2111, 2654
BseSI GKGCMC 1 cut(s) 1204
BseYI CCCAGC 4 cut(s) 855, 1125, 1682, 2259
BsgI GTGCAG 3 cut(s) 296, 2224, 2440
Bsh1236I CGCG 2 cut(s) 1047, 1393
BsiHKAI GWGCWC 3 cut(s) 1204, 1918, 2440
BsiHKCI CYCGRG 2 cut(s) 2112, 2428
BsiSI CCGG 2 cut(s) 1350, 2401
BslFI GGGAC 4 cut(s) 2114, 2210, 2242, 2568
BslI CCNNNNNNNGG 8 cut(s) 861, 1074, 1220, 1624, 1625, 1903, 1950, 2088
BsmAI GTCTC 7 cut(s) 204, 905, 1007, 1541, 1824, 2605, 2743
BsmFI GGGAC 4 cut(s) 2114, 2210, 2242, 2568
BsoBI CYCGRG 2 cut(s) 2112, 2428
Bsp119I TTCGAA 1 cut(s) 476
Bsp1286I GDGCHC 5 cut(s) 1204, 1516, 1918, 2440, 2765
Bsp1407I TGTACA 2 cut(s) 1237, 1725
Bsp143I GATC 5 cut(s) 232, 248, 400, 1776, 2140
BspACI CCGC 7 cut(s) 65, 212, 377, 718, 1045, 2534, 2747
BspFNI CGCG 2 cut(s) 1047, 1393
BspHI TCATGA 3 cut(s) 325, 1344, 2479
BspMAI CTGCAG 2 cut(s) 735, 1288
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 4 cut(s) 240, 395, 408, 1771
BspQI GCTCTTC 2 cut(s) 1644, 2770
BspT104I TTCGAA 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 1349
BsrGI TGTACA 2 cut(s) 1237, 1725
BsrI ACTGG 8 cut(s) 392, 694, 781, 1594, 1705, 1761, 2204, 2410
BssAI RCCGGY 1 cut(s) 1349
BssECI CCNNGG 5 cut(s) 860, 1619, 1792, 1869, 2758
BssMI GATC 5 cut(s) 232, 248, 400, 1776, 2140
BssNAI GTATAC 1 cut(s) 1612
BssT1I CCWWGG 3 cut(s) 860, 1619, 1792
Bst1107I GTATAC 1 cut(s) 1612
Bst2UI CCWGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
Bst4CI ACNGT 7 cut(s) 32, 593, 832, 839, 976, 1561, 2172
Bst6I CTCTTC 6 cut(s) 137, 897, 1644, 2004, 2603, 2770
BstAPI GCANNNNNTGC 2 cut(s) 572, 623
BstAUI TGTACA 2 cut(s) 1237, 1725
BstBAI YACGTR 1 cut(s) 1801
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 7 cut(s) 375, 507, 1470, 1482, 1689, 1758, 2693
BstF5I GGATG 7 cut(s) 109, 727, 1066, 1629, 1639, 2024, 2415
BstFNI CGCG 2 cut(s) 1047, 1393
BstHHI GCGC 2 cut(s) 207, 1393
BstKTI GATC 5 cut(s) 235, 251, 403, 1779, 2143
BstMAI GTCTC 7 cut(s) 204, 905, 1007, 1541, 1824, 2605, 2743
BstMBI GATC 5 cut(s) 232, 248, 400, 1776, 2140
BstMWI GCNNNNNNNGC 8 cut(s) 218, 379, 388, 572, 623, 1688, 2059, 2731
BstNI CCWGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
BstNSI RCATGY 3 cut(s) 1716, 2225, 2512
BstSCI CCNGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
BstSFI CTRYAG 3 cut(s) 576, 731, 1284
BstSLI GKGCMC 1 cut(s) 1204
BstUI CGCG 2 cut(s) 1047, 1393
BstV2I GAAGAC 1 cut(s) 2285
BstX2I RGATCY 2 cut(s) 400, 1776
BstXI CCANNNNNNTGG 1 cut(s) 1682
BstYI RGATCY 2 cut(s) 400, 1776
BstZ17I GTATAC 1 cut(s) 1612
Bsu36I CCTNAGG 1 cut(s) 1945
BsuI GTATCC 3 cut(s) 1249, 2056, 2578
BtgZI GCGATG 2 cut(s) 1105, 2118
BtrI CACGTC 2 cut(s) 408, 488
BtsCI GGATG 7 cut(s) 109, 727, 1066, 1629, 1639, 2024, 2415
BtsI GCAGTG 7 cut(s) 214, 1293, 2212, 2440, 2519, 2532, 2543
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 7 cut(s) 375, 507, 1470, 1482, 1689, 1758, 2693
CaiI CAGNNNCTG 4 cut(s) 244, 572, 700, 2491
CciI TCATGA 3 cut(s) 325, 1344, 2479
CfoI GCGC 2 cut(s) 207, 1393
Cfr10I RCCGGY 1 cut(s) 1349
Cfr13I GGNCC 8 cut(s) 965, 1703, 1858, 1975, 2079, 2256, 2555, 2781
CseI GACGC 1 cut(s) 1118
CspCI CAANNNNNGTGG 2 cut(s) 849, 884
DpnI GATC 5 cut(s) 234, 250, 402, 1778, 2142
DpnII GATC 5 cut(s) 232, 248, 400, 1776, 2140
EaeI YGGCCR 3 cut(s) 417, 1351, 1758
Eam1104I CTCTTC 6 cut(s) 137, 897, 1644, 2004, 2603, 2770
EarI CTCTTC 6 cut(s) 137, 897, 1644, 2004, 2603, 2770
Eco130I CCWWGG 3 cut(s) 860, 1619, 1792
Eco147I AGGCCT 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 1516, 2765
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 4 cut(s) 1858, 1975, 2256, 2555
Eco57I CTGAAG 1 cut(s) 2297
Eco81I CCTNAGG 1 cut(s) 1945
Eco88I CYCGRG 2 cut(s) 2112, 2428
EcoO109I RGGNCCY 2 cut(s) 2256, 2555
EcoRI GAATTC 1 cut(s) 659
EcoRII CCWGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 860, 1619, 1792
EcoT22I ATGCAT 1 cut(s) 1526
EcoT38I GRGCYC 2 cut(s) 1516, 2765
ErhI CCWWGG 3 cut(s) 860, 1619, 1792
FalI AAGNNNNNCTT 6 cut(s) 300, 332, 325, 357, 1235, 1267
FaqI GGGAC 4 cut(s) 2114, 2210, 2242, 2568
FauI CCCGC 2 cut(s) 711, 2740
FauNDI CATATG 1 cut(s) 633
FblI GTMKAC 1 cut(s) 1611
FokI GGATG 7 cut(s) 116, 734, 1073, 1636, 1646, 2031, 2422
FriOI GRGCYC 2 cut(s) 1516, 2765
FspBI CTAG 6 cut(s) 347, 446, 1620, 1653, 2244, 2267
GlaI GCGC 2 cut(s) 206, 1392
GsaI CCCAGC 4 cut(s) 859, 1129, 1686, 2263
GsuI CTGGAG 1 cut(s) 1308
HapII CCGG 2 cut(s) 1350, 2401
HgaI GACGC 1 cut(s) 1118
HhaI GCGC 2 cut(s) 207, 1393
Hin6I GCGC 2 cut(s) 205, 1391
HinP1I GCGC 2 cut(s) 205, 1391
HincII GTYRAC 1 cut(s) 491
HindII GTYRAC 1 cut(s) 491
HinfI GANTC 5 cut(s) 1025, 2135, 2272, 2488, 2579
HpaII CCGG 2 cut(s) 1350, 2401
HphI GGTGA 6 cut(s) 536, 605, 935, 1340, 2018, 2135
Hpy166II GTNNAC 5 cut(s) 491, 835, 1202, 1612, 1728
Hpy8I GTNNAC 5 cut(s) 491, 835, 1202, 1612, 1728
Hpy99I CGWCG 1 cut(s) 1134
HpyAV CCTTC 2 cut(s) 47, 118
HpyCH4III ACNGT 7 cut(s) 32, 593, 832, 839, 976, 1561, 2172
HpyCH4IV ACGT 6 cut(s) 407, 487, 493, 1678, 1800, 2322
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 379, 388, 572, 623, 1688, 2059, 2731
HpySE526I ACGT 6 cut(s) 407, 487, 493, 1678, 1800, 2322
HspAI GCGC 2 cut(s) 205, 1391
KflI GGGWCCC 1 cut(s) 2555
Kzo9I GATC 5 cut(s) 232, 248, 400, 1776, 2140
LguI GCTCTTC 2 cut(s) 1644, 2770
LmnI GCTCC 3 cut(s) 379, 502, 2530
LweI GCATC 7 cut(s) 97, 553, 604, 1624, 2009, 2095, 2400
MaeI CTAG 6 cut(s) 347, 446, 1620, 1653, 2244, 2267
MaeII ACGT 6 cut(s) 407, 487, 493, 1678, 1800, 2322
MaeIII GTNAC 9 cut(s) 26, 726, 824, 1077, 1150, 1288, 2207, 2232, 2518
MalI GATC 5 cut(s) 234, 250, 402, 1778, 2142
MboI GATC 5 cut(s) 232, 248, 400, 1776, 2140
MfeI CAATTG 1 cut(s) 2040
MflI RGATCY 2 cut(s) 400, 1776
MhlI GDGCHC 5 cut(s) 1204, 1516, 1918, 2440, 2765
MlsI TGGCCA 2 cut(s) 419, 1760
MluNI TGGCCA 2 cut(s) 419, 1760
MlyI GAGTC 2 cut(s) 2144, 2588
MmeI TCCRAC 2 cut(s) 748, 2562
Mox20I TGGCCA 2 cut(s) 419, 1760
Mph1103I ATGCAT 1 cut(s) 1526
MroXI GAANNNNTTC 2 cut(s) 1878, 2487
MscI TGGCCA 2 cut(s) 419, 1760
MseI TTAA 5 cut(s) 1140, 1167, 2226, 2373, 2394
MslI CAYNNNNRTG 3 cut(s) 561, 819, 1343
Msp20I TGGCCA 2 cut(s) 419, 1760
MspA1I CMGCKG 5 cut(s) 65, 1465, 1472, 1900, 2263
MspI CCGG 2 cut(s) 1350, 2401
MspR9I CCNGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
MunI CAATTG 1 cut(s) 2040
MvaI CCWGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
MvnI CGCG 2 cut(s) 1047, 1393
MwoI GCNNNNNNNGC 8 cut(s) 218, 379, 388, 572, 623, 1688, 2059, 2731
NdeI CATATG 1 cut(s) 633
NdeII GATC 5 cut(s) 232, 248, 400, 1776, 2140
NmuCI GTSAC 5 cut(s) 26, 1077, 1150, 1288, 2207
NsiI ATGCAT 1 cut(s) 1526
NspI RCATGY 3 cut(s) 1716, 2225, 2512
NspV TTCGAA 1 cut(s) 476
PagI TCATGA 3 cut(s) 325, 1344, 2479
PceI AGGCCT 1 cut(s) 337
PciI ACATGT 1 cut(s) 1712
PciSI GCTCTTC 2 cut(s) 1644, 2770
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 1684
PdmI GAANNNNTTC 2 cut(s) 1878, 2487
PfeI GAWTC 3 cut(s) 1025, 2272, 2488
PfoI TCCNGGA 1 cut(s) 1219
PleI GAGTC 2 cut(s) 2143, 2587
PpsI GAGTC 2 cut(s) 2143, 2587
Ppu21I YACGTR 1 cut(s) 1801
PpuMI RGGWCCY 2 cut(s) 2256, 2555
PscI ACATGT 1 cut(s) 1712
Psp1406I AACGTT 1 cut(s) 493
Psp5II RGGWCCY 2 cut(s) 2256, 2555
Psp6I CCWGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
PspFI CCCAGC 4 cut(s) 855, 1125, 1682, 2259
PspGI CCWGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
PspPI GGNCC 8 cut(s) 965, 1703, 1858, 1975, 2079, 2256, 2555, 2781
PspPPI RGGWCCY 2 cut(s) 2256, 2555
PsrI GAACNNNNNNTAC 2 cut(s) 1781, 1813
PstI CTGCAG 2 cut(s) 735, 1288
PstNI CAGNNNCTG 4 cut(s) 244, 572, 700, 2491
PsuI RGATCY 2 cut(s) 400, 1776
PvuII CAGCTG 4 cut(s) 1465, 1472, 1900, 2263
RseI CAYNNNNRTG 3 cut(s) 561, 819, 1343
SapI GCTCTTC 2 cut(s) 1644, 2770
SaqAI TTAA 5 cut(s) 1140, 1167, 2226, 2373, 2394
Sau3AI GATC 5 cut(s) 232, 248, 400, 1776, 2140
Sau96I GGNCC 8 cut(s) 965, 1703, 1858, 1975, 2079, 2256, 2555, 2781
ScaI AGTACT 2 cut(s) 1002, 1250
SchI GAGTC 2 cut(s) 2144, 2588
ScrFI CCNGG 5 cut(s) 1221, 1326, 1707, 1871, 2759
SduI GDGCHC 5 cut(s) 1204, 1516, 1918, 2440, 2765
SfaNI GCATC 7 cut(s) 97, 553, 604, 1624, 2009, 2095, 2400
SfcI CTRYAG 3 cut(s) 576, 731, 1284
SfuI TTCGAA 1 cut(s) 476
SinI GGWCC 4 cut(s) 1858, 1975, 2256, 2555
SmiMI CAYNNNNRTG 3 cut(s) 561, 819, 1343
SmlI CTYRAG 2 cut(s) 1814, 2667
SmoI CTYRAG 2 cut(s) 1814, 2667
SseBI AGGCCT 1 cut(s) 337
SsiI CCGC 7 cut(s) 65, 212, 377, 718, 1045, 2534, 2747
SspI AATATT 2 cut(s) 1540, 1879
SspMI CTAG 6 cut(s) 347, 446, 1620, 1653, 2244, 2267
StuI AGGCCT 1 cut(s) 337
StyD4I CCNGG 5 cut(s) 1219, 1324, 1705, 1869, 2757
StyI CCWWGG 3 cut(s) 860, 1619, 1792
TaaI ACNGT 7 cut(s) 32, 593, 832, 839, 976, 1561, 2172
TaiI ACGT 6 cut(s) 410, 490, 496, 1681, 1803, 2325
TaqI TCGA 4 cut(s) 118, 476, 742, 1920
TaqII GACCGA 1 cut(s) 1007
TatI WGTACW 6 cut(s) 984, 1000, 1237, 1248, 1725, 2505
TauI GCSGC 1 cut(s) 2536
TfiI GAWTC 3 cut(s) 1025, 2272, 2488
Tru1I TTAA 5 cut(s) 1140, 1167, 2226, 2373, 2394
Tru9I TTAA 5 cut(s) 1140, 1167, 2226, 2373, 2394
TseFI GTSAC 5 cut(s) 26, 1077, 1150, 1288, 2207
Tsp45I GTSAC 5 cut(s) 26, 1077, 1150, 1288, 2207
TspGWI ACGGA 1 cut(s) 146
VneI GTGCAC 1 cut(s) 1200
VpaK11BI GGWCC 4 cut(s) 1858, 1975, 2256, 2555
XapI RAATTY 7 cut(s) 659, 738, 1178, 1305, 2031, 2120, 2289
XceI RCATGY 3 cut(s) 1716, 2225, 2512
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmaJI CCTAGG 1 cut(s) 1619
XmiI GTMKAC 1 cut(s) 1611
XmnI GAANNNNTTC 2 cut(s) 1878, 2487
XspI CTAG 6 cut(s) 347, 446, 1620, 1653, 2244, 2267
ZrmI AGTACT 2 cut(s) 1002, 1250
Zsp2I ATGCAT 1 cut(s) 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.