MD05G1242000.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002001
Physical Location & Seq
Reverse (-)
37506740 .. 37507141
402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1242000.v1.1.491

Sequence Viewer

Length: 402 bp
ATGTGGGACAATTGGTTTCAACAGCACCCGAGCAGCGCACTTTATGGTATGGGCTGCAATCCAGATTATGTGATTTATCATGAGTTAGTTTTGACAATGAAGGAGTATATGCAGTGCGCCACTGCAGTGGAACCCCAATGGCTGGCTGAGTTCGGACCCATGTTCTGTCATGTGAAGGAGTCGGATAACTCAATGTTGGAGCACAAGAAGAGACAAAAGGGGGAGAAGACAGTGATGGAGGCGGATATAGAGAATCTGAGAAAGGCTCGAGCAGAGGCAGAGAAAGAAAGCAAGCAGAAGGAAAGGGAAAAGAGATCCAAGCAGCAGCAGCAGCAAGTCGGAATGCTGGGTTTGCACCAGGGCTCTTCTACATACTTGAGGCCTAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

134

Amino Acids

15.55

Weight (kDa)

8.4

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 6 - 53 4e-12 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 142
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 1 cut(s) 20
AjnI CCWGG 1 cut(s) 357
AleI CACNNNNGTG 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 2 cut(s) 28, 267
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 6 cut(s) 33, 54, 322, 325, 328, 331
AspLEI GCGC 2 cut(s) 38, 119
AspS9I GGNCC 1 cut(s) 155
AvaI CYCGRG 2 cut(s) 28, 267
AvaII GGWCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 6 cut(s) 41, 45, 334, 337, 340, 343
BccI CCATC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 6 cut(s) 34, 55, 323, 326, 329, 332
BlsI GCNGC 6 cut(s) 35, 56, 324, 327, 330, 333
Bme1390I CCNGG 1 cut(s) 359
Bme18I GGWCC 1 cut(s) 155
BmeT110I CYCGRG 2 cut(s) 28, 267
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 2 cut(s) 132, 157
BmrFI CCNGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 233
BplI GAGNNNNNCTC 2 cut(s) 250, 282
BpuEI CTTGAG 1 cut(s) 397
BsaJI CCNNGG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 358
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 138, 248
BseXI GCAGC 6 cut(s) 41, 45, 334, 337, 340, 343
BseYI CCCAGC 1 cut(s) 346
BshFI GGCC 1 cut(s) 382
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 28, 267
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 382
BsoBI CYCGRG 2 cut(s) 28, 267
Bsp1286I GDGCHC 2 cut(s) 204, 365
Bsp143I GATC 1 cut(s) 314
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 139, 249
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 132, 157
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 1 cut(s) 309
BspQI GCTCTTC 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 2 cut(s) 203, 370
BstC8I GCNNGC 2 cut(s) 144, 293
BstDEI CTNAG 3 cut(s) 147, 257, 384
BstHHI GCGC 2 cut(s) 38, 119
BstKTI GATC 1 cut(s) 317
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 1 cut(s) 314
BstMWI GCNNNNNNNGC 3 cut(s) 328, 331, 352
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 6 cut(s) 41, 45, 334, 337, 340, 343
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 1 cut(s) 314
BsuRI GGCC 1 cut(s) 382
BtsI GCAGTG 3 cut(s) 119, 120, 132
BtsIMutI CAGTG 4 cut(s) 119, 120, 132, 237
Cac8I GCNNGC 2 cut(s) 144, 293
CciI TCATGA 1 cut(s) 79
CfoI GCGC 2 cut(s) 38, 119
Cfr13I GGNCC 1 cut(s) 155
CviAII CATG 3 cut(s) 80, 160, 170
CviJI RGCY 7 cut(s) 54, 142, 146, 266, 363, 382, 396
CviKI_1 RGCY 7 cut(s) 54, 142, 146, 266, 363, 382, 396
DdeI CTNAG 3 cut(s) 147, 257, 384
DpnI GATC 1 cut(s) 316
DpnII GATC 1 cut(s) 314
Eam1104I CTCTTC 2 cut(s) 203, 370
EarI CTCTTC 2 cut(s) 203, 370
EciI GGCGGA 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 155
Eco88I CYCGRG 2 cut(s) 28, 267
EcoRII CCWGG 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 365
FaeI CATG 3 cut(s) 83, 163, 173
FaqI GGGAC 1 cut(s) 20
FatI CATG 3 cut(s) 79, 159, 169
Fnu4HI GCNGC 6 cut(s) 34, 55, 323, 326, 329, 332
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 6 cut(s) 34, 55, 323, 326, 329, 332
GlaI GCGC 2 cut(s) 37, 118
GluI GCNGC 6 cut(s) 34, 55, 323, 326, 329, 332
GsaI CCCAGC 1 cut(s) 350
HaeIII GGCC 1 cut(s) 382
HhaI GCGC 2 cut(s) 38, 119
Hin1II CATG 3 cut(s) 83, 163, 173
Hin6I GCGC 2 cut(s) 36, 117
HinP1I GCGC 2 cut(s) 36, 117
HinfI GANTC 2 cut(s) 179, 253
Hpy188I TCNGA 4 cut(s) 155, 184, 258, 341
Hpy188III TCNNGA 2 cut(s) 62, 80
HpyAV CCTTC 3 cut(s) 94, 169, 292
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4V TGCA 4 cut(s) 57, 112, 125, 355
HpyF10VI GCNNNNNNNGC 3 cut(s) 328, 331, 352
HpyF3I CTNAG 3 cut(s) 147, 257, 384
Hsp92II CATG 3 cut(s) 83, 163, 173
HspAI GCGC 2 cut(s) 36, 117
Kzo9I GATC 1 cut(s) 314
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 5 cut(s) 75, 128, 332, 344, 371
Lsp1109I GCAGC 6 cut(s) 41, 45, 334, 337, 340, 343
MalI GATC 1 cut(s) 316
MboI GATC 1 cut(s) 314
MboII GAAGA 3 cut(s) 220, 238, 357
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 204, 365
MluCI AATT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 3 cut(s) 162, 177, 319
MnlI CCTC 3 cut(s) 232, 268, 372
MslI CAYNNNNRTG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 359
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 328, 331, 352
NdeII GATC 1 cut(s) 314
NlaIII CATG 3 cut(s) 83, 163, 173
NlaIV GGNNCC 2 cut(s) 132, 157
OliI CACNNNNGTG 1 cut(s) 125
PaeR7I CTCGAG 1 cut(s) 267
PagI TCATGA 1 cut(s) 79
PceI AGGCCT 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 370
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 6 cut(s) 35, 56, 324, 327, 330, 333
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 132, 157
PspPI GGNCC 1 cut(s) 155
PspXI VCTCGAGB 1 cut(s) 267
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 370
SatI GCNGC 6 cut(s) 34, 55, 323, 326, 329, 332
Sau3AI GATC 1 cut(s) 314
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 2 cut(s) 204, 365
SfcI CTRYAG 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 267, 376
SmoI CTYRAG 2 cut(s) 267, 376
Sse9I AATT 1 cut(s) 10
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 232
TaqI TCGA 1 cut(s) 268
TasI AATT 1 cut(s) 10
TfiI GAWTC 1 cut(s) 253
TscAI CASTG 4 cut(s) 119, 127, 132, 237
TseI GCWGC 6 cut(s) 33, 54, 322, 325, 328, 331
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 4 cut(s) 119, 127, 132, 237
Van91I CCANNNNNTGG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 155
XhoI CTCGAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.