Rh4CG330500

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002001
Physical Location & Seq
Reverse (-)
59120737 .. 59124638
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG330500.1

Sequence Viewer

Length: 804 bp
ATGAACCATAGCTCCCGTGAAGGTTGGAACAACAAGGAAGATGGTAGCAGTACGGAATATGGAAAGAGGTGCGGAGAAGTCAATCTTTCTATCTTAGAAAATTTGTTCCATCTGCGCCATAAGTTTGCTCGGTTACTTGGCTATTCAAACTATGCTAACTATGCTGTTGATCTTAGAATGGCAAAGACGCCCACAAAGCAGTGGAAACTACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCCCTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTCCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTCGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTAGGGCCCATATTCTTCTCTGTGAAGGATTCAGATACATCGCTATTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACATTTATGGGGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAGGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAGGCCAAAGAAACTTAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

267

Amino Acids

30.98

Weight (kDa)

9.25

Isoelectric Point (pI)

53.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 119 - 196 7.6e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 72, 575
AclWI GGATC 2 cut(s) 279, 738
AcsI RAATTY 2 cut(s) 100, 762
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 2 cut(s) 52, 536
AfiI CCNNNNNNNGG 1 cut(s) 435
AgsI TTSAA 4 cut(s) 147, 307, 403, 768
AjnI CCWGG 1 cut(s) 772
AleI CACNNNNGTG 1 cut(s) 554
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 12, 292, 365, 798
AluI AGCT 4 cut(s) 12, 292, 365, 798
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 1 cut(s) 634
AlwI GGATC 2 cut(s) 279, 738
AoxI GGCC 3 cut(s) 338, 584, 782
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 4 cut(s) 292, 751, 754, 757
ApoI RAATTY 2 cut(s) 100, 762
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 238, 584, 585
AsuHPI GGTGA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 588
BaeI ACNNNNGTAYC 2 cut(s) 200, 233
BanII GRGCYC 1 cut(s) 588
BbsI GAAGAC 2 cut(s) 314, 662
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 4 cut(s) 279, 763, 766, 769
BccI CCATC 4 cut(s) 35, 117, 456, 670
BciT130I CCWGG 1 cut(s) 774
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 3 cut(s) 273, 296, 396
BfmI CTRYAG 1 cut(s) 552
BglII AGATCT 1 cut(s) 300
BisI GCNGC 4 cut(s) 293, 752, 755, 758
BlsI GCNGC 4 cut(s) 294, 753, 756, 759
Bme1390I CCNGG 1 cut(s) 774
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 3 cut(s) 238, 584, 585
BmiI GGNNCC 3 cut(s) 239, 561, 586
BmrFI CCNGG 1 cut(s) 774
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 2 cut(s) 429, 473
BmuI ACTGGG 1 cut(s) 444
BpiI GAAGAC 2 cut(s) 314, 662
BplI GAGNNNNNCTC 4 cut(s) 451, 483, 679, 711
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 188
BsaXI ACNNNNNCTCC 3 cut(s) 26, 551, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 2 cut(s) 233, 439
BseBI CCWGG 1 cut(s) 774
BseGI GGATG 3 cut(s) 444, 454, 488
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 677
BseNI ACTGG 2 cut(s) 233, 439
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 4 cut(s) 279, 763, 766, 769
BseYI CCCAGC 1 cut(s) 288
BshFI GGCC 3 cut(s) 340, 586, 784
BsiHKAI GWGCWC 1 cut(s) 469
BslFI GGGAC 2 cut(s) 239, 251
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 1 cut(s) 634
BsmFI GGGAC 2 cut(s) 239, 251
BsnI GGCC 3 cut(s) 340, 586, 784
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 2 cut(s) 469, 588
Bsp143I GATC 4 cut(s) 169, 284, 300, 743
BspACI CCGC 2 cut(s) 72, 575
BspANI GGCC 3 cut(s) 340, 586, 784
BspCNI CTCAG 1 cut(s) 678
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 239, 561, 586
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 2 cut(s) 279, 738
BsrI ACTGG 2 cut(s) 233, 439
BssMI GATC 4 cut(s) 169, 284, 300, 743
BssNI GRCGYC 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 1 cut(s) 567
Bst6I CTCTTC 2 cut(s) 632, 645
BstACI GRCGYC 1 cut(s) 188
BstDEI CTNAG 4 cut(s) 94, 173, 686, 794
BstF5I GGATG 3 cut(s) 444, 454, 488
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 4 cut(s) 172, 287, 303, 746
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 4 cut(s) 169, 284, 300, 743
BstMWI GCNNNNNNNGC 4 cut(s) 161, 196, 628, 757
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 2 cut(s) 254, 541
BstSCI CCNGG 1 cut(s) 772
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 4 cut(s) 279, 763, 766, 769
BstV2I GAAGAC 2 cut(s) 314, 662
BstX2I RGATCY 3 cut(s) 284, 300, 743
BstXI CCANNNNNNTGG 1 cut(s) 556
BstYI RGATCY 3 cut(s) 284, 300, 743
BsuRI GGCC 3 cut(s) 340, 586, 784
BtgZI GCGATG 1 cut(s) 603
BtsCI GGATG 3 cut(s) 444, 454, 488
BtsI GCAGTG 5 cut(s) 206, 469, 548, 549, 561
BtsIMutI CAGTG 8 cut(s) 206, 311, 345, 469, 548, 549, 561, 572
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 238, 584, 585
CseI GACGC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 51, 535
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 4 cut(s) 251, 444, 509, 538
CviQI GTAC 2 cut(s) 51, 535
DdeI CTNAG 4 cut(s) 94, 173, 686, 794
DpnI GATC 4 cut(s) 171, 286, 302, 745
DpnII GATC 4 cut(s) 169, 284, 300, 743
Eam1104I CTCTTC 2 cut(s) 632, 645
EarI CTCTTC 2 cut(s) 632, 645
EciI GGCGGA 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 772
EcoT38I GRGCYC 1 cut(s) 588
FaeI CATG 4 cut(s) 254, 447, 512, 541
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 2 cut(s) 239, 251
FatI CATG 4 cut(s) 250, 443, 508, 537
Fnu4HI GCNGC 4 cut(s) 293, 752, 755, 758
FokI GGATG 3 cut(s) 441, 451, 495
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 4 cut(s) 293, 752, 755, 758
FspBI CTAG 3 cut(s) 273, 296, 396
GlaI GCGC 1 cut(s) 116
GluI GCNGC 4 cut(s) 293, 752, 755, 758
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 3 cut(s) 340, 586, 784
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 4 cut(s) 254, 447, 512, 541
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 608, 682
HphI GGTGA 1 cut(s) 292
Hpy188I TCNGA 3 cut(s) 459, 613, 687
Hpy188III TCNNGA 4 cut(s) 304, 429, 491, 509
HpyAV CCTTC 4 cut(s) 14, 397, 523, 598
HpyCH4III ACNGT 1 cut(s) 567
HpyCH4V TGCA 4 cut(s) 250, 486, 541, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 196, 628, 757
HpyF3I CTNAG 4 cut(s) 94, 173, 686, 794
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 4 cut(s) 254, 447, 512, 541
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 169, 284, 300, 743
LmnI GCTCC 3 cut(s) 17, 559, 628
LpnPI CCDG 9 cut(s) 214, 302, 338, 354, 420, 442, 504, 759, 786
Lsp1109I GCAGC 4 cut(s) 279, 763, 766, 769
LweI GCATC 2 cut(s) 429, 473
MaeI CTAG 3 cut(s) 273, 296, 396
MaeIII GTNAC 2 cut(s) 132, 261
MalI GATC 4 cut(s) 171, 286, 302, 745
MboI GATC 4 cut(s) 169, 284, 300, 743
MboII GAAGA 6 cut(s) 50, 319, 586, 649, 662, 667
MflI RGATCY 3 cut(s) 284, 300, 743
MhlI GDGCHC 2 cut(s) 469, 588
MluCI AATT 4 cut(s) 100, 388, 424, 762
MmeI TCCRAC 1 cut(s) 5
MnlI CCTC 6 cut(s) 60, 215, 271, 697, 720, 774
MseI TTAA 2 cut(s) 255, 423
MslI CAYNNNNRTG 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 774
MwoI GCNNNNNNNGC 4 cut(s) 161, 196, 628, 757
NdeII GATC 4 cut(s) 169, 284, 300, 743
NlaIII CATG 4 cut(s) 254, 447, 512, 541
NlaIV GGNNCC 3 cut(s) 239, 561, 586
NspI RCATGY 2 cut(s) 254, 541
OliI CACNNNNGTG 1 cut(s) 554
PagI TCATGA 1 cut(s) 508
PfeI GAWTC 2 cut(s) 608, 682
PflFI GACNNNGTC 1 cut(s) 497
PkrI GCNGC 4 cut(s) 294, 753, 756, 759
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 239, 561, 586
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 3 cut(s) 238, 584, 585
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 3 cut(s) 284, 300, 743
PsyI GACNNNGTC 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 2 cut(s) 52, 536
RsaNI GTAC 2 cut(s) 51, 535
RseI CAYNNNNRTG 1 cut(s) 554
SaqAI TTAA 2 cut(s) 255, 423
SatI GCNGC 4 cut(s) 293, 752, 755, 758
Sau3AI GATC 4 cut(s) 169, 284, 300, 743
Sau96I GGNCC 3 cut(s) 238, 584, 585
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 2 cut(s) 469, 588
SetI ASST 7 cut(s) 14, 25, 71, 282, 294, 367, 800
SfaNI GCATC 2 cut(s) 429, 473
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
Sse9I AATT 4 cut(s) 100, 388, 424, 762
SsiI CCGC 2 cut(s) 72, 575
SspMI CTAG 3 cut(s) 273, 296, 396
StyD4I CCNGG 1 cut(s) 772
TaaI ACNGT 1 cut(s) 567
TaqI TCGA 1 cut(s) 220
TasI AATT 4 cut(s) 100, 388, 424, 762
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 2 cut(s) 608, 682
Tru1I TTAA 2 cut(s) 255, 423
Tru9I TTAA 2 cut(s) 255, 423
TscAI CASTG 8 cut(s) 206, 318, 352, 469, 548, 556, 561, 572
TseI GCWGC 4 cut(s) 292, 751, 754, 757
TspDTI ATGAA 2 cut(s) 17, 525
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 8 cut(s) 206, 318, 352, 469, 548, 556, 561, 572
Tth111I GACNNNGTC 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 2 cut(s) 100, 762
XceI RCATGY 2 cut(s) 254, 541
XspI CTAG 3 cut(s) 273, 296, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.