Rw5G014220

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002001
Physical Location & Seq
Forward (+)
16904296 .. 16907885
3590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014220.1

Sequence Viewer

Length: 3246 bp
ATGGTGAAATCGGAACTCGAGAAGAGCGGAGGAGGTGGAGGATTACATGTTCGCGGGACGGAGAGAGTTGTTTTCAGGCCGCCGGATAAGAAGAAGAAATTAGGGTTTGATGGAGGTTTCAGGAGATATAAGGGTAGGTATGATGATGGGGATGGTAGTGAAAGAAGAGATTACAGAGATGGTGGTGGTGGTGGTGGTAGTAGGAGTAGGGTGAGAGGAAGATATGAGGAAGATGGATATGGTGGTGATTATGGAAGAAAGAAGAGTAGATATGAAGGTTCGAAGAGGGGAGCTCGATCTGTTGCTTCTGCCTGGGATTGTATCTCTTACTCTCCGGTTCAGATACGGGCTTCTGGACAGTCAGTCAAGTCTTCGGGCTCGGGACGTGGTGGGAGTTCTCATCAGCTTGATAGTACGCTAGGAGAAGAGGAGGGTGATTCGGTTGAAGGGAGATATGAGATTGGTGAGAGCATGCGTTTAGAGATGGAATATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCACAGCGGTTGATACGAGTGATAGTTCGTCTTTGTTTTATGGGGATGATGCTTTTTGTCGAAAGAAGGAAACGGAGTTGGCGAAGAGACTGGCTCGGAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAGGTTGTCTCAGTCACTTCTGCGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAACAAAGTTATACTTCTTGTTCATGATACAAGGCCTCCTTTCCTGGATGGGAGAGTTGTTTTTACCAAGCAAGCGGAGCCGGTTATGCCAGTAAAGGATCCCACATCTGATATGGCCATAATTTCACGCAAAGGGTCTGCTCTAGTTAGGGAAATCCATCAAAAGCAAAGTTCGAATAAATCACGTCAACGTTTTTGGGAGCTTTCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAGAAAACAGCGGAGCATATTGATGCAGATACTGCTGTAGTAGGTAAAGACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCTCAGCATATGAAGAATGGGGAGGCTGTGAGTGAATTCACAAAGTCAAAAACCATTTCCGAACAACGCCAGTATCTGCCTATATATTCTGTGCGGGATGAGTTACTGCAGAAAATTCGAGAAAATCAAGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGTTACACTGTGAACGGTATAGTCGGTTGCACCCAGCCAAGACGTGTGGCAGCCATGAGTGTTGCTAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGAGAAAGTGGGCTATGCTATTCGTTTTGAGGATGTGACGGGGCCAAAAACTGTAATAAAGTACATGACTGATGGAGTACTTATGCGTGAAACACTTAGAGATTCTGACCTTGACAAGTACCGTGTAGTTGTAATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATTTTGAAAAAAGTCGTCGCCCAGCGTCGTGATTTTAAGCTCATTGTGACATCTGCTACTCTTAATGCTCAGAAATTTGCAGATTTCTTTGGAGGTGCACCTATTTTTCCCATTCCTGGAAGAACATTTCCTGTACATAAGAAGTACTTGGATACCCCATGTGAAGATTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTGGCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTTGCAGAGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGGGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTTTACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGCTGTACACAGAGAGTGCCTACCTAAATGAAATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTCAAGTCTCTGAAGGTTGATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCTGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAAAATGGTGGAGTTCCCATTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTAGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCAGAGTCCGATCACCTGACATTCTACAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTACAAAAGGCTAGAGAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACGTTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATGGGATGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACAGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGACAATCTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAGCAAGCAGAGGGAGAGGGAAAAGAGGTATAAACAGCAGCAGCAGCAGCAGCAAGTCTCATTGGCGGGTTTGCACCAGGGCTCTTCTACATATCTGAGGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1081

Amino Acids

121.27

Weight (kDa)

7.02

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 387 - 539 8.2e-09 DEAD/DEAH box helicase
AAA_22 PF13401 395 - 528 3.8e-06 AAA domain
Helicase_C PF00271 585 - 714 1.6e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 779 - 804 6.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 805 - 864 2e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 922 - 998 5.4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 27
AccII CGCG 1 cut(s) 54
AciI CCGC 9 cut(s) 27, 54, 80, 536, 815, 989, 1156, 2972, 3191
AclI AACGTT 1 cut(s) 931
AclWI GGATC 3 cut(s) 833, 846, 2209
AcoI YGGCCR 3 cut(s) 855, 1789, 2196
AcsI RAATTY 7 cut(s) 1097, 1176, 1616, 1743, 2469, 2558, 2727
AcuI CTGAAG 2 cut(s) 2282, 2735
AfiI CCNNNNNNNGG 7 cut(s) 1512, 1658, 2062, 2063, 2341, 2388, 2526
AflIII ACRYGT 3 cut(s) 46, 1303, 2150
AgsI TTSAA 8 cut(s) 446, 1045, 1549, 1718, 1805, 2081, 2725, 2795
AhdI GACNNNNNGTC 1 cut(s) 362
AjiI CACGTC 3 cut(s) 386, 926, 1304
AjnI CCWGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
Alw21I GWGCWC 4 cut(s) 295, 1642, 2356, 2878
Alw26I GTCTC 7 cut(s) 610, 675, 1343, 1979, 2262, 3043, 3187
Alw44I GTGCAC 1 cut(s) 1638
AlwI GGATC 3 cut(s) 833, 846, 2209
AlwNI CAGNNNCTG 3 cut(s) 1010, 1138, 2929
Ama87I CYCGRG 4 cut(s) 17, 379, 2550, 2866
ApaLI GTGCAC 1 cut(s) 1638
ApoI RAATTY 7 cut(s) 1097, 1176, 1616, 1743, 2469, 2558, 2727
Asp700I GAANNNNTTC 2 cut(s) 2316, 2925
AspA2I CCTAGG 1 cut(s) 2057
AspS9I GGNCC 8 cut(s) 1403, 2141, 2296, 2413, 2517, 2694, 2993, 3225
AsuII TTCGAA 2 cut(s) 281, 914
AvaI CYCGRG 4 cut(s) 17, 379, 2550, 2866
AvaII GGWCC 4 cut(s) 2296, 2413, 2694, 2993
AvrII CCTAGG 1 cut(s) 2057
AxyI CCTNAGG 1 cut(s) 2383
BaeGI GKGCMC 1 cut(s) 1642
BalI TGGCCA 2 cut(s) 857, 2198
BamHI GGATCC 1 cut(s) 838
BanII GRGCYC 5 cut(s) 295, 380, 1954, 2356, 3209
BbsI GAAGAC 2 cut(s) 363, 2723
Bbv12I GWGCWC 4 cut(s) 295, 1642, 2356, 2878
BcgI CGANNNNNNTGC 2 cut(s) 1160, 1194
BciT130I CCWGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
BciVI GTATCC 3 cut(s) 1687, 2494, 3016
BcoDI GTCTC 7 cut(s) 610, 675, 1343, 1979, 2262, 3043, 3187
BfaI CTAG 7 cut(s) 419, 884, 2058, 2091, 2453, 2682, 2705
BfmI CTRYAG 3 cut(s) 1014, 1169, 1722
BfuI GTATCC 3 cut(s) 1687, 2494, 3016
BlnI CCTAGG 1 cut(s) 2057
BlpI GCTNAGC 1 cut(s) 1065
BmcAI AGTACT 2 cut(s) 1440, 1688
Bme1390I CCNGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
Bme18I GGWCC 4 cut(s) 2296, 2413, 2694, 2993
BmeRI GACNNNNNGTC 1 cut(s) 362
BmeT110I CYCGRG 4 cut(s) 17, 379, 2550, 2866
BmgBI CACGTC 3 cut(s) 386, 926, 1304
BmgT120I GGNCC 8 cut(s) 1403, 2141, 2296, 2413, 2517, 2694, 2993, 3225
BmrFI CCNGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
BmrI ACTGGG 2 cut(s) 2148, 2853
BmsI GCATC 8 cut(s) 568, 991, 1042, 2062, 2447, 2533, 2838, 2882
BmuI ACTGGG 2 cut(s) 2148, 2853
BpiI GAAGAC 2 cut(s) 363, 2723
BpmI CTGGAG 1 cut(s) 1746
Bpu1102I GCTNAGC 1 cut(s) 1065
Bpu14I TTCGAA 2 cut(s) 281, 914
BpuEI CTTGAG 2 cut(s) 2237, 3090
BsaAI YACGTR 1 cut(s) 2239
BsaJI CCNNGG 5 cut(s) 312, 2057, 2230, 2307, 3202
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 4 cut(s) 760, 790, 2960, 2990
Bsc4I CCNNNNNNNGG 7 cut(s) 1512, 1658, 2062, 2063, 2341, 2388, 2526
Bse118I RCCGGY 2 cut(s) 820, 1787
Bse1I ACTGG 9 cut(s) 624, 830, 1132, 1219, 2032, 2143, 2199, 2642, 2848
Bse21I CCTNAGG 1 cut(s) 2383
BseBI CCWGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
BseDI CCNNGG 5 cut(s) 312, 2057, 2230, 2307, 3202
BseLI CCNNNNNNNGG 7 cut(s) 1512, 1658, 2062, 2063, 2341, 2388, 2526
BseNI ACTGG 9 cut(s) 624, 830, 1132, 1219, 2032, 2143, 2199, 2642, 2848
BseRI GAGGAG 7 cut(s) 45, 443, 1811, 1840, 1843, 2549, 3092
BseSI GKGCMC 1 cut(s) 1642
BseYI CCCAGC 4 cut(s) 1293, 1563, 2120, 2697
BsgI GTGCAG 2 cut(s) 734, 2662
Bsh1236I CGCG 1 cut(s) 54
BsiHKAI GWGCWC 4 cut(s) 295, 1642, 2356, 2878
BsiHKCI CYCGRG 4 cut(s) 17, 379, 2550, 2866
BsiSI CCGG 5 cut(s) 83, 335, 821, 1788, 2839
BslFI GGGAC 6 cut(s) 70, 396, 2552, 2648, 2680, 3006
BslI CCNNNNNNNGG 7 cut(s) 1512, 1658, 2062, 2063, 2341, 2388, 2526
BsmAI GTCTC 7 cut(s) 610, 675, 1343, 1979, 2262, 3043, 3187
BsmFI GGGAC 6 cut(s) 70, 396, 2552, 2648, 2680, 3006
BsoBI CYCGRG 4 cut(s) 17, 379, 2550, 2866
Bsp119I TTCGAA 2 cut(s) 281, 914
Bsp1286I GDGCHC 7 cut(s) 295, 380, 1642, 1954, 2356, 2878, 3209
Bsp1407I TGTACA 2 cut(s) 1675, 2163
Bsp143I GATC 5 cut(s) 296, 686, 838, 2214, 2578
Bsp1720I GCTNAGC 1 cut(s) 1065
BspACI CCGC 9 cut(s) 27, 54, 80, 536, 815, 989, 1156, 2972, 3191
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 3 cut(s) 763, 1782, 2917
BspMAI CTGCAG 2 cut(s) 1173, 1726
BspPI GGATC 3 cut(s) 833, 846, 2209
BspQI GCTCTTC 4 cut(s) 17, 2082, 2442, 3214
BspT104I TTCGAA 2 cut(s) 281, 914
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 820, 1787
BsrGI TGTACA 2 cut(s) 1675, 2163
BsrI ACTGG 9 cut(s) 624, 830, 1132, 1219, 2032, 2143, 2199, 2642, 2848
BssAI RCCGGY 2 cut(s) 820, 1787
BssECI CCNNGG 5 cut(s) 312, 2057, 2230, 2307, 3202
BssMI GATC 5 cut(s) 296, 686, 838, 2214, 2578
BssT1I CCWWGG 2 cut(s) 2057, 2230
Bst2UI CCWGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
Bst4CI ACNGT 9 cut(s) 360, 503, 1031, 1270, 1277, 1414, 1484, 1999, 2610
BstAPI GCANNNNNTGC 2 cut(s) 1010, 1061
BstAUI TGTACA 2 cut(s) 1675, 2163
BstBAI YACGTR 1 cut(s) 2239
BstBI TTCGAA 2 cut(s) 281, 914
BstC8I GCNNGC 7 cut(s) 473, 813, 1908, 1920, 2127, 2196, 3131
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 5 cut(s) 299, 689, 841, 2217, 2581
BstMAI GTCTC 7 cut(s) 610, 675, 1343, 1979, 2262, 3043, 3187
BstMBI GATC 5 cut(s) 296, 686, 838, 2214, 2578
BstMWI GCNNNNNNNGC 9 cut(s) 817, 826, 1010, 1061, 2126, 2497, 3169, 3172, 3175
BstNI CCWGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
BstNSI RCATGY 5 cut(s) 50, 475, 2154, 2663, 2950
BstSCI CCNGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
BstSFI CTRYAG 3 cut(s) 1014, 1169, 1722
BstSLI GKGCMC 1 cut(s) 1642
BstUI CGCG 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 363, 2723
BstX2I RGATCY 2 cut(s) 838, 2214
BstXI CCANNNNNNTGG 1 cut(s) 2120
BstYI RGATCY 2 cut(s) 838, 2214
Bsu36I CCTNAGG 1 cut(s) 2383
BsuI GTATCC 3 cut(s) 1687, 2494, 3016
BtgZI GCGATG 1 cut(s) 2556
BtrI CACGTC 3 cut(s) 386, 926, 1304
BtsI GCAGTG 6 cut(s) 1731, 2650, 2878, 2957, 2970, 2981
Cac8I GCNNGC 7 cut(s) 473, 813, 1908, 1920, 2127, 2196, 3131
CaiI CAGNNNCTG 3 cut(s) 1010, 1138, 2929
CciI TCATGA 3 cut(s) 763, 1782, 2917
Cfr10I RCCGGY 2 cut(s) 820, 1787
Cfr13I GGNCC 8 cut(s) 1403, 2141, 2296, 2413, 2517, 2694, 2993, 3225
CseI GACGC 1 cut(s) 1556
CspCI CAANNNNNGTGG 2 cut(s) 1287, 1322
DpnI GATC 5 cut(s) 298, 688, 840, 2216, 2580
DpnII GATC 5 cut(s) 296, 686, 838, 2214, 2578
DriI GACNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 3 cut(s) 855, 1789, 2196
Eam1105I GACNNNNNGTC 1 cut(s) 362
Ecl136II GAGCTC 2 cut(s) 293, 2354
Eco130I CCWWGG 2 cut(s) 2057, 2230
Eco147I AGGCCT 1 cut(s) 775
Eco24I GRGCYC 5 cut(s) 295, 380, 1954, 2356, 3209
Eco32I GATATC 1 cut(s) 1771
Eco47I GGWCC 4 cut(s) 2296, 2413, 2694, 2993
Eco53kI GAGCTC 2 cut(s) 293, 2354
Eco57I CTGAAG 2 cut(s) 2282, 2735
Eco81I CCTNAGG 1 cut(s) 2383
Eco88I CYCGRG 4 cut(s) 17, 379, 2550, 2866
EcoICRI GAGCTC 2 cut(s) 293, 2354
EcoO109I RGGNCCY 2 cut(s) 2694, 2993
EcoRI GAATTC 1 cut(s) 1097
EcoRII CCWGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
EcoRV GATATC 1 cut(s) 1771
EcoT14I CCWWGG 2 cut(s) 2057, 2230
EcoT22I ATGCAT 1 cut(s) 1964
EcoT38I GRGCYC 5 cut(s) 295, 380, 1954, 2356, 3209
ErhI CCWWGG 2 cut(s) 2057, 2230
FalI AAGNNNNNCTT 6 cut(s) 738, 770, 763, 795, 1673, 1705
FaqI GGGAC 6 cut(s) 70, 396, 2552, 2648, 2680, 3006
FauI CCCGC 3 cut(s) 47, 1149, 3184
FauNDI CATATG 1 cut(s) 1071
FriOI GRGCYC 5 cut(s) 295, 380, 1954, 2356, 3209
FspBI CTAG 7 cut(s) 419, 884, 2058, 2091, 2453, 2682, 2705
GsaI CCCAGC 4 cut(s) 1297, 1567, 2124, 2701
GsuI CTGGAG 1 cut(s) 1746
HapII CCGG 5 cut(s) 83, 335, 821, 1788, 2839
HgaI GACGC 1 cut(s) 1556
HincII GTYRAC 1 cut(s) 929
HindII GTYRAC 1 cut(s) 929
HinfI GANTC 6 cut(s) 437, 1463, 2573, 2710, 2926, 3017
HpaII CCGG 5 cut(s) 83, 335, 821, 1788, 2839
Hpy166II GTNNAC 5 cut(s) 929, 1273, 1640, 2050, 2166
Hpy8I GTNNAC 5 cut(s) 929, 1273, 1640, 2050, 2166
Hpy99I CGWCG 2 cut(s) 1562, 1572
HpyAV CCTTC 5 cut(s) 269, 440, 518, 589, 2257
HpyCH4III ACNGT 9 cut(s) 360, 503, 1031, 1270, 1277, 1414, 1484, 1999, 2610
HpyCH4IV ACGT 8 cut(s) 385, 925, 931, 1303, 2116, 2238, 2720, 2760
HpyF10VI GCNNNNNNNGC 9 cut(s) 817, 826, 1010, 1061, 2126, 2497, 3169, 3172, 3175
HpySE526I ACGT 8 cut(s) 385, 925, 931, 1303, 2116, 2238, 2720, 2760
KflI GGGWCCC 1 cut(s) 2993
Kzo9I GATC 5 cut(s) 296, 686, 838, 2214, 2578
LguI GCTCTTC 4 cut(s) 17, 2082, 2442, 3214
LmnI GCTCC 6 cut(s) 290, 817, 940, 991, 2351, 2968
LweI GCATC 8 cut(s) 568, 991, 1042, 2062, 2447, 2533, 2838, 2882
MaeI CTAG 7 cut(s) 419, 884, 2058, 2091, 2453, 2682, 2705
MaeII ACGT 8 cut(s) 385, 925, 931, 1303, 2116, 2238, 2720, 2760
MalI GATC 5 cut(s) 298, 688, 840, 2216, 2580
MbiI CCGCTC 1 cut(s) 27
MboI GATC 5 cut(s) 296, 686, 838, 2214, 2578
MfeI CAATTG 1 cut(s) 2478
MflI RGATCY 2 cut(s) 838, 2214
MhlI GDGCHC 7 cut(s) 295, 380, 1642, 1954, 2356, 2878, 3209
MlsI TGGCCA 2 cut(s) 857, 2198
MluNI TGGCCA 2 cut(s) 857, 2198
MlyI GAGTC 2 cut(s) 2582, 3026
MmeI TCCRAC 2 cut(s) 1186, 3000
Mox20I TGGCCA 2 cut(s) 857, 2198
Mph1103I ATGCAT 1 cut(s) 1964
MroXI GAANNNNTTC 2 cut(s) 2316, 2925
MscI TGGCCA 2 cut(s) 857, 2198
MseI TTAA 5 cut(s) 1578, 1605, 2664, 2811, 2832
MslI CAYNNNNRTG 3 cut(s) 999, 1257, 1781
Msp20I TGGCCA 2 cut(s) 857, 2198
MspA1I CMGCKG 6 cut(s) 536, 989, 1903, 1910, 2338, 2701
MspI CCGG 5 cut(s) 83, 335, 821, 1788, 2839
MspR9I CCNGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
MunI CAATTG 1 cut(s) 2478
MvaI CCWGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 9 cut(s) 817, 826, 1010, 1061, 2126, 2497, 3169, 3172, 3175
NdeI CATATG 1 cut(s) 1071
NdeII GATC 5 cut(s) 296, 686, 838, 2214, 2578
NmuCI GTSAC 7 cut(s) 497, 675, 1396, 1515, 1588, 1726, 2645
NsiI ATGCAT 1 cut(s) 1964
NspI RCATGY 5 cut(s) 50, 475, 2154, 2663, 2950
NspV TTCGAA 2 cut(s) 281, 914
PaeI GCATGC 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 3 cut(s) 763, 1782, 2917
PceI AGGCCT 1 cut(s) 775
PciI ACATGT 2 cut(s) 46, 2150
PciSI GCTCTTC 4 cut(s) 17, 2082, 2442, 3214
PcsI WCGNNNNNNNCGW 2 cut(s) 608, 2122
PdmI GAANNNNTTC 2 cut(s) 2316, 2925
PfeI GAWTC 4 cut(s) 437, 1463, 2710, 2926
PfoI TCCNGGA 2 cut(s) 783, 1657
PleI GAGTC 2 cut(s) 2581, 3025
PpsI GAGTC 2 cut(s) 2581, 3025
Ppu21I YACGTR 1 cut(s) 2239
PpuMI RGGWCCY 2 cut(s) 2694, 2993
PscI ACATGT 2 cut(s) 46, 2150
Psp124BI GAGCTC 2 cut(s) 295, 2356
Psp1406I AACGTT 1 cut(s) 931
Psp5II RGGWCCY 2 cut(s) 2694, 2993
Psp6I CCWGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
PspFI CCCAGC 4 cut(s) 1293, 1563, 2120, 2697
PspGI CCWGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
PspPI GGNCC 8 cut(s) 1403, 2141, 2296, 2413, 2517, 2694, 2993, 3225
PspPPI RGGWCCY 2 cut(s) 2694, 2993
PsrI GAACNNNNNNTAC 2 cut(s) 2219, 2251
PstI CTGCAG 2 cut(s) 1173, 1726
PstNI CAGNNNCTG 3 cut(s) 1010, 1138, 2929
PsuI RGATCY 2 cut(s) 838, 2214
PvuII CAGCTG 4 cut(s) 1903, 1910, 2338, 2701
RseI CAYNNNNRTG 3 cut(s) 999, 1257, 1781
SacI GAGCTC 2 cut(s) 295, 2356
SapI GCTCTTC 4 cut(s) 17, 2082, 2442, 3214
SaqAI TTAA 5 cut(s) 1578, 1605, 2664, 2811, 2832
Sau3AI GATC 5 cut(s) 296, 686, 838, 2214, 2578
Sau96I GGNCC 8 cut(s) 1403, 2141, 2296, 2413, 2517, 2694, 2993, 3225
ScaI AGTACT 2 cut(s) 1440, 1688
SchI GAGTC 2 cut(s) 2582, 3026
ScrFI CCNGG 7 cut(s) 313, 785, 1659, 1764, 2145, 2309, 3203
SduI GDGCHC 7 cut(s) 295, 380, 1642, 1954, 2356, 2878, 3209
SfaNI GCATC 8 cut(s) 568, 991, 1042, 2062, 2447, 2533, 2838, 2882
SfcI CTRYAG 3 cut(s) 1014, 1169, 1722
Sfr274I CTCGAG 1 cut(s) 17
SfuI TTCGAA 2 cut(s) 281, 914
SinI GGWCC 4 cut(s) 2296, 2413, 2694, 2993
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 999, 1257, 1781
SmlI CTYRAG 3 cut(s) 17, 2252, 3105
SmoI CTYRAG 3 cut(s) 17, 2252, 3105
SphI GCATGC 1 cut(s) 475
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 9 cut(s) 27, 54, 80, 536, 815, 989, 1156, 2972, 3191
SspI AATATT 2 cut(s) 1978, 2317
SspMI CTAG 7 cut(s) 419, 884, 2058, 2091, 2453, 2682, 2705
SstI GAGCTC 2 cut(s) 295, 2356
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 7 cut(s) 311, 783, 1657, 1762, 2143, 2307, 3201
StyI CCWWGG 2 cut(s) 2057, 2230
TaaI ACNGT 9 cut(s) 360, 503, 1031, 1270, 1277, 1414, 1484, 1999, 2610
TaiI ACGT 8 cut(s) 388, 928, 934, 1306, 2119, 2241, 2723, 2763
TaqI TCGA 7 cut(s) 18, 281, 295, 589, 914, 1180, 2358
TatI WGTACW 6 cut(s) 1422, 1438, 1675, 1686, 2163, 2943
TauI GCSGC 2 cut(s) 82, 2974
TfiI GAWTC 4 cut(s) 437, 1463, 2710, 2926
Tru1I TTAA 5 cut(s) 1578, 1605, 2664, 2811, 2832
Tru9I TTAA 5 cut(s) 1578, 1605, 2664, 2811, 2832
TseFI GTSAC 7 cut(s) 497, 675, 1396, 1515, 1588, 1726, 2645
Tsp45I GTSAC 7 cut(s) 497, 675, 1396, 1515, 1588, 1726, 2645
TspGWI ACGGA 2 cut(s) 74, 617
VneI GTGCAC 1 cut(s) 1638
VpaK11BI GGWCC 4 cut(s) 2296, 2413, 2694, 2993
XapI RAATTY 7 cut(s) 1097, 1176, 1616, 1743, 2469, 2558, 2727
XceI RCATGY 5 cut(s) 50, 475, 2154, 2663, 2950
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 17
XmaJI CCTAGG 1 cut(s) 2057
XmnI GAANNNNTTC 2 cut(s) 2316, 2925
XspI CTAG 7 cut(s) 419, 884, 2058, 2091, 2453, 2682, 2705
ZrmI AGTACT 2 cut(s) 1440, 1688
Zsp2I ATGCAT 1 cut(s) 1964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.