RLG00000006958

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002001
Physical Location & Seq
Forward (+)
12159846 .. 12168039
8194 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006958

Sequence Viewer

Length: 4002 bp
ATGATTTTGAAGAAAAAAGTCAACAAAGCTCCGAAGAAACCCCACTCTTCTACCACACAGGGTTTTATATCACTAATTGCGATGGAGAATAGAGGAAACATTGAAGCTATTAAGCTGGACAAGACGACGGCGACATTGGAACCCGAGAATAGCAGTGGTGGGGGATTATTTGTTCCTGGGAAAGATCGAGTTGTGTATAGACCTCCCGAGAGGAAATCTCTCTTAGGTTTAGATGTCCTTGCCATTGCAAAACGGGAAGAGTCTAAGGTTGAGGGTGGATTCAAAACCCCAAGAGAAAGAGTTGCATCGGTTGTCACATCTCTAGAGGAGGAGGAAAACTCCGTGTCTGGTGTAATAGATGAAACAGGGAGTGATATATCCACTGGTGTATGCAGTCATTCCAAGAGGCGATATCGTGGAATATCTGCAAGTGATACACCTCAGACAGAAAGTACAGTAACGGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGCGAACGTTTTCGGACAGATGTTTTGGCCACTTCTAGTGGAAGTTCCCACAGTGTTAGGTCTCAGAGTTCATATAATAGGGATGATCGTGGTAGTGACAGAAGAGATCACAAGGGTAGGTCTGACAGGGACGACAATGATAGTGAGAGAAGAGATTACCAAGATAGTAGTAGAGGCGATAGTTGGAGGGAGAGAAAAAGATATGGCAGTGATAGTAAGGATTATTATGGTCGAAGAGAAGAACGTGGTCGATATGAACAAGAATATGGTGGAGAATACGAAAGAAAGCGGGGTAGATACGAAGGTTCAAAGAGGACACCTGGCCGCTCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGACCCCACGACGTGATGGTTACTCTAATACCAGTAGGCGTCATCAACCATCACGATCCCCTATGCTACTCGGGGCCTCACCTGATGCACGACTAGTATCTCCTTGGCTGGGTGGGAATACACCTCATTCTGGTTCTGCTGCTTCTCCATGGGATCACATATCTCCATCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCAGCTCACATTTTCTTCGGAAAATTCACAATCATTCCAGGGTGGAGAAGCATTTAATACTGATTCGACTGGAGAACACAATTATGAAATTAGTGAAAGCATGCGTGCAGAGATGGAATACAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGAAATACAATGTATGATACGAGTGATAGCTCATCATTGTTTTTCGGAGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCTCAGATGACTGCTGATAATGCGCAGTGGGAGGATCGCCAACTTTTGAGATCAGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAATTTGATGATGAAGACGAACGCAAAGTTATTCTTCTTGTACATGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCGATAAAGGATCCTACATCTGATATGGCCATAATATCACGCAAAGGGTCTACTTTGGTTAGGGAAATCCATGAGAAGCAAAGTTCTAACAAATCACGCCAACGTTTTTGGGAGCTTGCTGGTTCCAAACTTGGGGATATCCTTGGTGTTGAGAAAACTGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGATGCTAAGTTTGCCCAACATATGAAGAATGGGGAAGCTGTCAGTGATTTTGCAAAGTCAAAAACCCTCTCACAGCAACGGCAGTATTTGCCCATATATTCTGTGAGGGATGATTTGTTGCAGGTGATTCGAGAAAATCAAGTTATCGTTGTTGTTGGAGAAACCGGTTCTGGAAAGACAACTCAACTGACACAGTATCTGCATGAAGATGGATACACGGTAAATGGTATAGTAGGTTGCACTCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTCAGTGAGGAAATGGAGACTGAACTTGGTGATAAAGTCGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTTCTTTTGCGCGAAACTCTAAGGGATTCTGATCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTGTTTGGAATTTTAAAAAAAGTGGTTGCCCAACGTCGTGATTTCAAGCTCATTGTGACCTCTGCAACTCTAAATGCTCAGAAGTTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCATGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGTGACATCCTCATCTTCATGACTGGCCAAGATGAGATCGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTATCTCCTCCTCTAATAAAGCGGTGCCAAAACTGCTGATACTTCCTATATATTCACAGCTGCCTGCTGACCTGCAAGCGAAGATTTTCCAAAGAGCTGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCGGAGACCTCTCTGACTGTCGATGGAATCTTTTACGTCATTGACACAGGTTATGGCAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGTGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACGGAGAATGCATACCTGAATGAAATGCTCCCAAGTCCTGTGCCGGAAATCCAGAGGACTAACCTTGGTAATGTGGTCTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGATCCACCTCCCCAAGATAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTGGGTGCTCTTAACAACGTGGGCGGCTTGACAGACCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTTTTGATGGGTGCTGAGCTGGGATGCTTGGACGAGGTTTTGACAATTGTGTCCATGCTCTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATCGGGCAGAGGAGAGTGATGCTGCACGAGAGAAATTCTCTATTCCTGAATCCGATCACTTGACGTTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCACTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCTTACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTTGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCAGAGTTGGGGCCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTGTTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACAGCTATGGAGCAAGAGATGGAGAATCTGCGAAAGGCTCAAGCAGAGGCAGAGATAGACAACAAGCAGAAGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAACAAGCGCTCTTCTACATATTTGAGGCCAAAGAAACTCGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1334

Amino Acids

150.26

Weight (kDa)

6.41

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 645 - 793 2.2e-08 DEAD/DEAH box helicase
Helicase_C PF00271 840 - 969 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1034 - 1059 5.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1060 - 1119 1.8e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1177 - 1254 4.1e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1927
AasI GACNNNNNNGTC 1 cut(s) 3247
Acc16I TGCGCA 1 cut(s) 1425
Acc36I ACCTGC 3 cut(s) 1750, 1927, 2688
AccB1I GGYRCC 1 cut(s) 2632
AccBSI CCGCTC 2 cut(s) 831, 2596
AccI GTMKAC 2 cut(s) 1643, 2814
AccII CGCG 1 cut(s) 2214
AciI CCGC 7 cut(s) 793, 829, 2596, 2630, 2747, 2894, 3135
AclI AACGTT 3 cut(s) 511, 1696, 3363
AclWI GGATC 9 cut(s) 915, 1026, 1443, 1598, 1611, 3056, 3069, 3453, 3912
AcoI YGGCCR 5 cut(s) 531, 826, 1555, 1620, 2554
AcsI RAATTY 6 cut(s) 1126, 1487, 2307, 2389, 3323, 3936
AcuI CTGAAG 1 cut(s) 2727
AcyI GRCGYC 1 cut(s) 904
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 9 cut(s) 454, 488, 1471, 1527, 2189, 2406, 2453, 2930, 3710
AfeI AGCGCT 1 cut(s) 3962
AfiI CCNNNNNNNGG 8 cut(s) 974, 995, 1725, 2277, 2827, 2828, 3291, 3609
AflII CTTAAG 1 cut(s) 3026
AflIII ACRYGT 1 cut(s) 2915
AgeI ACCGGT 1 cut(s) 1979
AhlI ACTAGT 1 cut(s) 958
AjiI CACGTC 2 cut(s) 878, 2296
AjnI CCWGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
AjuI GAANNNNNNNTTGG 2 cut(s) 3066, 3098
AleI CACNNNNGTG 2 cut(s) 2294, 3728
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
Alw21I GWGCWC 2 cut(s) 3121, 3643
Alw26I GTCTC 8 cut(s) 498, 570, 860, 1407, 2108, 2286, 2744, 3808
AlwI GGATC 9 cut(s) 915, 1026, 1443, 1598, 1611, 3056, 3069, 3453, 3912
AlwNI CAGNNNCTG 3 cut(s) 1460, 1775, 2014
Ama87I CYCGRG 3 cut(s) 143, 206, 935
Aor51HI AGCGCT 1 cut(s) 3962
ApaI GGGCCC 1 cut(s) 3762
ApoI RAATTY 6 cut(s) 1126, 1487, 2307, 2389, 3323, 3936
ArsI GACNNNNNNTTYG 4 cut(s) 511, 543, 2285, 2317
AsiGI ACCGGT 1 cut(s) 1979
Asp700I GAANNNNTTC 3 cut(s) 513, 2693, 3039
AspLEI GCGC 3 cut(s) 1426, 2214, 3963
AspS9I GGNCC 9 cut(s) 939, 1098, 2168, 2906, 3178, 3282, 3412, 3758, 3759
AsuHPI GGTGA 7 cut(s) 936, 1799, 1951, 2138, 2509, 2543, 3466
AsuII TTCGAA 1 cut(s) 1357
AvaI CYCGRG 3 cut(s) 143, 206, 935
AvaII GGWCC 4 cut(s) 1098, 2906, 3178, 3412
BaeGI GKGCMC 1 cut(s) 3762
BaeI ACNNNNGTAYC 6 cut(s) 793, 826, 1461, 1494, 3374, 3407
BalI TGGCCA 3 cut(s) 533, 1622, 2556
BamHI GGATCC 2 cut(s) 1603, 3061
BanI GGYRCC 1 cut(s) 2632
BanII GRGCYC 2 cut(s) 2719, 3762
BauI CACGAG 1 cut(s) 3315
BbsI GAAGAC 3 cut(s) 1506, 3488, 3836
Bbv12I GWGCWC 2 cut(s) 3121, 3643
BceAI ACGGC 2 cut(s) 144, 1910
BcgI CGANNNNNNTGC 2 cut(s) 1925, 1959
BciT130I CCWGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
BciVI GTATCC 2 cut(s) 1052, 2021
BcoDI GTCTC 8 cut(s) 498, 570, 860, 1407, 2108, 2286, 2744, 3808
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 7 cut(s) 323, 540, 959, 2823, 3447, 3470, 3570
BfmI CTRYAG 1 cut(s) 3726
BfoI RGCGCY 1 cut(s) 3964
BfrI CTTAAG 1 cut(s) 3026
BfuAI ACCTGC 3 cut(s) 1750, 1927, 2688
BfuI GTATCC 2 cut(s) 1052, 2021
BglII AGATCT 2 cut(s) 1070, 3474
BlpI GCTNAGC 1 cut(s) 3213
Bme1390I CCNGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
Bme18I GGWCC 4 cut(s) 1098, 2906, 3178, 3412
BmeT110I CYCGRG 3 cut(s) 143, 206, 935
BmgBI CACGTC 2 cut(s) 878, 2296
BmgT120I GGNCC 9 cut(s) 939, 1098, 2168, 2906, 3178, 3282, 3412, 3758, 3759
BmrFI CCNGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
BmrI ACTGGG 3 cut(s) 847, 2175, 3618
BmuI ACTGGG 3 cut(s) 847, 2175, 3618
BpiI GAAGAC 3 cut(s) 1506, 3488, 3836
BpmI CTGGAG 4 cut(s) 1020, 1194, 2511, 3648
Bpu1102I GCTNAGC 1 cut(s) 3213
Bpu14I TTCGAA 1 cut(s) 1357
BpuEI CTTGAG 2 cut(s) 3002, 3855
BsaAI YACGTR 1 cut(s) 1049
BsaBI GATNNNNATC 2 cut(s) 1063, 2232
BsaHI GRCGYC 1 cut(s) 904
BsaI GGTCTC 4 cut(s) 570, 860, 2286, 2744
BsaJI CCNNGG 9 cut(s) 176, 968, 1013, 1141, 1735, 2063, 2423, 2995, 3148
BsaWI WCCGGW 1 cut(s) 1979
BsaXI ACNNNNNCTCC 6 cut(s) 1525, 1555, 2509, 2539, 3725, 3755
Bsc4I CCNNNNNNNGG 8 cut(s) 974, 995, 1725, 2277, 2827, 2828, 3291, 3609
Bse118I RCCGGY 1 cut(s) 1979
Bse3DI GCAATG 1 cut(s) 243
Bse8I GATNNNNATC 2 cut(s) 1063, 2232
BseBI CCWGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
BseDI CCNNGG 9 cut(s) 176, 968, 1013, 1141, 1735, 2063, 2423, 2995, 3148
BseJI GATNNNNATC 2 cut(s) 1063, 2232
BseLI CCNNNNNNNGG 8 cut(s) 974, 995, 1725, 2277, 2827, 2828, 3291, 3609
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 9 cut(s) 455, 581, 1400, 1418, 1464, 1743, 2390, 3204, 3273
BseRI GAGGAG 7 cut(s) 341, 344, 878, 1769, 2605, 2608, 3314
BseSI GKGCMC 1 cut(s) 3762
BseYI CCCAGC 4 cut(s) 973, 2885, 3217, 3462
BsgI GTGCAG 4 cut(s) 1230, 1499, 2508, 3297
Bsh1236I CGCG 1 cut(s) 2214
BshNI GGYRCC 1 cut(s) 2632
BshTI ACCGGT 1 cut(s) 1979
BsiHKAI GWGCWC 2 cut(s) 3121, 3643
BsiHKCI CYCGRG 3 cut(s) 143, 206, 935
BsiSI CCGG 2 cut(s) 1980, 2975
BslFI GGGAC 4 cut(s) 647, 1084, 3413, 3425
BslI CCNNNNNNNGG 8 cut(s) 974, 995, 1725, 2277, 2827, 2828, 3291, 3609
BsmAI GTCTC 8 cut(s) 498, 570, 860, 1407, 2108, 2286, 2744, 3808
BsmFI GGGAC 4 cut(s) 647, 1084, 3413, 3425
BsmI GAATGC 1 cut(s) 2944
Bso31I GGTCTC 4 cut(s) 570, 860, 2286, 2744
BsoBI CYCGRG 3 cut(s) 143, 206, 935
Bsp119I TTCGAA 1 cut(s) 1357
Bsp120I GGGCCC 1 cut(s) 3758
Bsp1286I GDGCHC 4 cut(s) 2719, 3121, 3643, 3762
Bsp1407I TGTACA 3 cut(s) 1525, 2451, 2928
Bsp1720I GCTNAGC 1 cut(s) 3213
Bsp19I CCATGG 1 cut(s) 1013
BspACI CCGC 7 cut(s) 793, 829, 2596, 2630, 2747, 2894, 3135
BspCNI CTCAG 9 cut(s) 454, 580, 1399, 1417, 1465, 1744, 2389, 3205, 3272
BspFNI CGCG 1 cut(s) 2214
BspHI TCATGA 2 cut(s) 2547, 3682
BspMAI CTGCAG 1 cut(s) 3730
BspMI ACCTGC 3 cut(s) 1750, 1927, 2688
BspPI GGATC 9 cut(s) 915, 1026, 1443, 1598, 1611, 3056, 3069, 3453, 3912
BspQI GCTCTTC 2 cut(s) 2847, 3970
BspT104I TTCGAA 1 cut(s) 1357
BspT107I GGYRCC 1 cut(s) 2632
BspTI CTTAAG 1 cut(s) 3026
BspTNI GGTCTC 4 cut(s) 570, 860, 2286, 2744
BsrBI CCGCTC 2 cut(s) 831, 2596
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 1979
BsrGI TGTACA 3 cut(s) 1525, 2451, 2928
BssAI RCCGGY 1 cut(s) 1979
BssECI CCNNGG 9 cut(s) 176, 968, 1013, 1141, 1735, 2063, 2423, 2995, 3148
BssNAI GTATAC 1 cut(s) 2815
BssNI GRCGYC 1 cut(s) 904
BssSI CACGAG 1 cut(s) 3315
BssT1I CCWWGG 6 cut(s) 968, 1013, 1735, 2063, 2995, 3148
Bst1107I GTATAC 1 cut(s) 2815
Bst2BI CACGAG 1 cut(s) 3315
Bst2UI CCWGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
Bst4CI ACNGT 8 cut(s) 457, 557, 2010, 2035, 2451, 2456, 2764, 3741
BstACI GRCGYC 1 cut(s) 904
BstAFI CTTAAG 1 cut(s) 3026
BstAPI GCANNNNNTGC 2 cut(s) 1775, 1903
BstAUI TGTACA 3 cut(s) 1525, 2451, 2928
BstBAI YACGTR 1 cut(s) 1049
BstBI TTCGAA 1 cut(s) 1357
BstDSI CCRYGG 1 cut(s) 1013
BstFNI CGCG 1 cut(s) 2214
BstH2I RGCGCY 1 cut(s) 3964
BstHHI GCGC 3 cut(s) 1426, 2214, 3963
BstMAI GTCTC 8 cut(s) 498, 570, 860, 1407, 2108, 2286, 2744, 3808
BstNI CCWGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
BstNSI RCATGY 4 cut(s) 1207, 2919, 3428, 3715
BstSCI CCNGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
BstSFI CTRYAG 1 cut(s) 3726
BstSLI GKGCMC 1 cut(s) 3762
BstUI CGCG 1 cut(s) 2214
BstV2I GAAGAC 3 cut(s) 1506, 3488, 3836
BstX2I RGATCY 6 cut(s) 1070, 1603, 3061, 3458, 3474, 3917
BstXI CCANNNNNNTGG 2 cut(s) 2885, 3730
BstYI RGATCY 6 cut(s) 1070, 1603, 3061, 3458, 3474, 3917
BstZ17I GTATAC 1 cut(s) 2815
BsuI GTATCC 2 cut(s) 1052, 2021
BtgI CCRYGG 1 cut(s) 1013
BtgZI GCGATG 2 cut(s) 95, 3777
BtrI CACGTC 2 cut(s) 878, 2296
BtsI GCAGTG 8 cut(s) 160, 718, 1433, 3380, 3643, 3722, 3723, 3735
BveI ACCTGC 3 cut(s) 1750, 1927, 2688
CaiI CAGNNNCTG 3 cut(s) 1460, 1775, 2014
CciI TCATGA 2 cut(s) 2547, 3682
CfoI GCGC 3 cut(s) 1426, 2214, 3963
Cfr10I RCCGGY 1 cut(s) 1979
Cfr13I GGNCC 9 cut(s) 939, 1098, 2168, 2906, 3178, 3282, 3412, 3758, 3759
CseI GACGC 2 cut(s) 893, 1041
CsiI ACCWGGT 1 cut(s) 1087
Csp6I GTAC 9 cut(s) 453, 487, 1470, 1526, 2188, 2405, 2452, 2929, 3709
CspAI ACCGGT 1 cut(s) 1979
CspCI CAANNNNNGTGG 4 cut(s) 2052, 2087, 3726, 3761
CviQI GTAC 9 cut(s) 453, 487, 1470, 1526, 2188, 2405, 2452, 2929, 3709
DraI TTTAAA 1 cut(s) 2313
DraIII CACNNNGTG 1 cut(s) 878
DrdI GACNNNNNNGTC 1 cut(s) 3247
DseDI GACNNNNNNGTC 1 cut(s) 3247
EaeI YGGCCR 5 cut(s) 531, 826, 1555, 1620, 2554
Eco130I CCWWGG 6 cut(s) 968, 1013, 1735, 2063, 2995, 3148
Eco24I GRGCYC 2 cut(s) 2719, 3762
Eco31I GGTCTC 4 cut(s) 570, 860, 2286, 2744
Eco32I GATATC 2 cut(s) 413, 1732
Eco47I GGWCC 4 cut(s) 1098, 2906, 3178, 3412
Eco47III AGCGCT 1 cut(s) 3962
Eco57I CTGAAG 1 cut(s) 2727
Eco88I CYCGRG 3 cut(s) 143, 206, 935
EcoO109I RGGNCCY 3 cut(s) 939, 1098, 3758
EcoRII CCWGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
EcoRV GATATC 2 cut(s) 413, 1732
EcoT14I CCWWGG 6 cut(s) 968, 1013, 1735, 2063, 2995, 3148
EcoT22I ATGCAT 1 cut(s) 2944
EcoT38I GRGCYC 2 cut(s) 2719, 3762
ErhI CCWWGG 6 cut(s) 968, 1013, 1735, 2063, 2995, 3148
FalI AAGNNNNNCTT 2 cut(s) 1503, 1535
FaqI GGGAC 4 cut(s) 647, 1084, 3413, 3425
FauI CCCGC 2 cut(s) 786, 2887
FauNDI CATATG 1 cut(s) 1836
FblI GTMKAC 2 cut(s) 1643, 2814
FriOI GRGCYC 2 cut(s) 2719, 3762
FspBI CTAG 7 cut(s) 323, 540, 959, 2823, 3447, 3470, 3570
FspI TGCGCA 1 cut(s) 1425
GlaI GCGC 3 cut(s) 1425, 2213, 3962
GsaI CCCAGC 4 cut(s) 977, 2889, 3221, 3466
GsuI CTGGAG 4 cut(s) 1020, 1194, 2511, 3648
HaeII RGCGCY 1 cut(s) 3964
HapII CCGG 2 cut(s) 1980, 2975
HgaI GACGC 2 cut(s) 893, 1041
HhaI GCGC 3 cut(s) 1426, 2214, 3963
Hin1I GRCGYC 1 cut(s) 904
Hin6I GCGC 3 cut(s) 1424, 2212, 3961
HinP1I GCGC 3 cut(s) 1424, 2212, 3961
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HpaII CCGG 2 cut(s) 1980, 2975
HphI GGTGA 7 cut(s) 936, 1799, 1951, 2138, 2509, 2543, 3466
Hpy166II GTNNAC 6 cut(s) 22, 1644, 2405, 2815, 2931, 3367
Hpy8I GTNNAC 6 cut(s) 22, 1644, 2405, 2815, 2931, 3367
Hpy99I CGWCG 3 cut(s) 130, 879, 2337
HpyAV CCTTC 9 cut(s) 800, 1250, 1321, 2477, 3050, 3571, 3697, 3772, 3895
HpyCH4III ACNGT 8 cut(s) 457, 557, 2010, 2035, 2451, 2456, 2764, 3741
Hsp92I GRCGYC 1 cut(s) 904
HspAI GCGC 3 cut(s) 1424, 2212, 3961
LguI GCTCTTC 2 cut(s) 2847, 3970
LmnI GCTCC 7 cut(s) 34, 1705, 1756, 2964, 3733, 3802, 3841
MabI ACCWGGT 1 cut(s) 1087
MaeI CTAG 7 cut(s) 323, 540, 959, 2823, 3447, 3470, 3570
MaeIII GTNAC 9 cut(s) 313, 457, 599, 884, 2161, 2353, 2531, 3021, 3435
MbiI CCGCTC 2 cut(s) 831, 2596
MfeI CAATTG 1 cut(s) 3243
MflI RGATCY 6 cut(s) 1070, 1603, 3061, 3458, 3474, 3917
MhlI GDGCHC 4 cut(s) 2719, 3121, 3643, 3762
MlsI TGGCCA 3 cut(s) 533, 1622, 2556
MluNI TGGCCA 3 cut(s) 533, 1622, 2556
MlyI GAGTC 2 cut(s) 269, 2106
MmeI TCCRAC 3 cut(s) 668, 1951, 3780
Mox20I TGGCCA 3 cut(s) 533, 1622, 2556
Mph1103I ATGCAT 1 cut(s) 2944
MroXI GAANNNNTTC 3 cut(s) 513, 2693, 3039
MscI TGGCCA 3 cut(s) 533, 1622, 2556
MseI TTAA 8 cut(s) 111, 1158, 2312, 2442, 3027, 3123, 3429, 3597
MslI CAYNNNNRTG 5 cut(s) 1185, 2022, 2294, 2546, 3728
Msp20I TGGCCA 3 cut(s) 533, 1622, 2556
MspA1I CMGCKG 2 cut(s) 2668, 3466
MspCI CTTAAG 1 cut(s) 3026
MspI CCGG 2 cut(s) 1980, 2975
MspR9I CCNGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
MunI CAATTG 1 cut(s) 3243
Mva1269I GAATGC 1 cut(s) 2944
MvaI CCWGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
MvnI CGCG 1 cut(s) 2214
NcoI CCATGG 1 cut(s) 1013
NdeI CATATG 1 cut(s) 1836
NmeAIII GCCGAG 2 cut(s) 1583, 3972
NmuCI GTSAC 5 cut(s) 313, 599, 2353, 2531, 3021
NsbI TGCGCA 1 cut(s) 1425
NsiI ATGCAT 1 cut(s) 2944
NspI RCATGY 4 cut(s) 1207, 2919, 3428, 3715
NspV TTCGAA 1 cut(s) 1357
OliI CACNNNNGTG 2 cut(s) 2294, 3728
PaeI GCATGC 1 cut(s) 1207
PagI TCATGA 2 cut(s) 2547, 3682
PaqCI CACCTGC 1 cut(s) 1927
PciI ACATGT 1 cut(s) 2915
PciSI GCTCTTC 2 cut(s) 2847, 3970
PctI GAATGC 1 cut(s) 2944
PdmI GAANNNNTTC 3 cut(s) 513, 2693, 3039
PfeI GAWTC 8 cut(s) 279, 1166, 1942, 2228, 2772, 3338, 3782, 3856
PfoI TCCNGGA 2 cut(s) 1548, 2908
PinAI ACCGGT 1 cut(s) 1979
PleI GAGTC 2 cut(s) 268, 2105
PpsI GAGTC 2 cut(s) 268, 2105
Ppu21I YACGTR 1 cut(s) 1049
PpuMI RGGWCCY 1 cut(s) 1098
PscI ACATGT 1 cut(s) 2915
Psp1406I AACGTT 3 cut(s) 511, 1696, 3363
Psp5II RGGWCCY 1 cut(s) 1098
Psp6I CCWGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
PspFI CCCAGC 4 cut(s) 973, 2885, 3217, 3462
PspGI CCWGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
PspOMI GGGCCC 1 cut(s) 3758
PspPI GGNCC 9 cut(s) 939, 1098, 2168, 2906, 3178, 3282, 3412, 3758, 3759
PspPPI RGGWCCY 1 cut(s) 1098
PstI CTGCAG 1 cut(s) 3730
PstNI CAGNNNCTG 3 cut(s) 1460, 1775, 2014
PsuI RGATCY 6 cut(s) 1070, 1603, 3061, 3458, 3474, 3917
PvuII CAGCTG 2 cut(s) 2668, 3466
RsaI GTAC 9 cut(s) 454, 488, 1471, 1527, 2189, 2406, 2453, 2930, 3710
RsaNI GTAC 9 cut(s) 453, 487, 1470, 1526, 2188, 2405, 2452, 2929, 3709
RseI CAYNNNNRTG 5 cut(s) 1185, 2022, 2294, 2546, 3728
SapI GCTCTTC 2 cut(s) 2847, 3970
SaqAI TTAA 8 cut(s) 111, 1158, 2312, 2442, 3027, 3123, 3429, 3597
Sau96I GGNCC 9 cut(s) 939, 1098, 2168, 2906, 3178, 3282, 3412, 3758, 3759
SchI GAGTC 2 cut(s) 269, 2106
ScrFI CCNGG 9 cut(s) 177, 825, 1089, 1142, 1550, 2424, 2529, 2910, 3948
SduI GDGCHC 4 cut(s) 2719, 3121, 3643, 3762
SexAI ACCWGGT 1 cut(s) 1087
SfcI CTRYAG 1 cut(s) 3726
SfuI TTCGAA 1 cut(s) 1357
SinI GGWCC 4 cut(s) 1098, 2906, 3178, 3412
SmiMI CAYNNNNRTG 5 cut(s) 1185, 2022, 2294, 2546, 3728
SmlI CTYRAG 3 cut(s) 3017, 3026, 3870
SmoI CTYRAG 3 cut(s) 3017, 3026, 3870
SpeI ACTAGT 1 cut(s) 958
SphI GCATGC 1 cut(s) 1207
SsiI CCGC 7 cut(s) 793, 829, 2596, 2630, 2747, 2894, 3135
SspI AATATT 2 cut(s) 2743, 3082
SspMI CTAG 7 cut(s) 323, 540, 959, 2823, 3447, 3470, 3570
StyD4I CCNGG 9 cut(s) 175, 823, 1087, 1140, 1548, 2422, 2527, 2908, 3946
StyI CCWWGG 6 cut(s) 968, 1013, 1735, 2063, 2995, 3148
TaaI ACNGT 8 cut(s) 457, 557, 2010, 2035, 2451, 2456, 2764, 3741
TaqI TCGA 9 cut(s) 187, 736, 754, 1169, 1357, 1945, 2568, 2766, 3394
TatI WGTACW 6 cut(s) 452, 1525, 2187, 2451, 2928, 3708
TauI GCSGC 2 cut(s) 831, 3138
TfiI GAWTC 8 cut(s) 279, 1166, 1942, 2228, 2772, 3338, 3782, 3856
Tru1I TTAA 8 cut(s) 111, 1158, 2312, 2442, 3027, 3123, 3429, 3597
Tru9I TTAA 8 cut(s) 111, 1158, 2312, 2442, 3027, 3123, 3429, 3597
TseFI GTSAC 5 cut(s) 313, 599, 2353, 2531, 3021
Tsp45I GTSAC 5 cut(s) 313, 599, 2353, 2531, 3021
TspGWI ACGGA 3 cut(s) 331, 476, 2948
Vha464I CTTAAG 1 cut(s) 3026
VpaK11BI GGWCC 4 cut(s) 1098, 2906, 3178, 3412
XapI RAATTY 6 cut(s) 1126, 1487, 2307, 2389, 3323, 3936
XbaI TCTAGA 1 cut(s) 322
XceI RCATGY 4 cut(s) 1207, 2919, 3428, 3715
XmiI GTMKAC 2 cut(s) 1643, 2814
XmnI GAANNNNTTC 3 cut(s) 513, 2693, 3039
XspI CTAG 7 cut(s) 323, 540, 959, 2823, 3447, 3470, 3570
Zsp2I ATGCAT 1 cut(s) 2944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.