FvH4_4g24000

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002001
Physical Location & Seq
Reverse (-)
26280004 .. 26288922
8919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24000.t1

Sequence Viewer

Length: 3924 bp
ATGGAGAATAAAGGAAACACTGAAGCTATTAAGCTGGACAAGACCACGGCGACATTGGAACCTGAGAAGAGCAGCGGCGGGGGATTGTTTGTTCCTGGGAAGGATCGAGTTGTGTATAGGCCTCCGGAGCGGAAATCGCTCTTAGGTTTAGATGTCCTTGCTATTGCAAAACGAGAAGAGTCTAAGGCTGAGGGTGGATTCAAAGCCCCAAAAGATAGAGTTACTTCTGTTGTGGCATCTCTAGAGGAGGAGGAAAATAACTCTGAGTCTGGTATAATAGATGACACAGGTGATGATATATCCACTGGTGTACGCAGTCACAGTAAGAGGCGATATCGTGGAATATCTGCAAACGATACACCTCGAACAGAAAGTACGGTAACAGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGTGAACGTTTTCGGACAGATGTTTCGGCCTCGCCTAGCGGAAGTTACCACAGTGTTAGGTCACAAATTTCGTATAACAGGGATGATCGTGGTAGTGAGAGACGAGATTACAGGGGTAGGTCTGACAGGGACGACCATGATAGTGAGAGAAGAGATTATCAAGATAGTAGTAGGGGCGATAGTTGGAGGGAGAGAAAACGATATGGTAGTGATAATAAGGATTATAATGGTCGAAGAGAAGAACGTGGCCGATATGAACAAGATTATGGTGGAGAATACGAAAGAAAGCGGGGTAGATACGAAGGTTCAAGGAGGACACCTGGCCGGTCTGATTGGGATGATGGGAGATGGGAATGGGAAGAGACACCACGACGTGATGGTTACTCTAATACCAGTAGGCATCATCAACCATCACGGTCCCCTATGCTACTTGGGGCCTCACCTGATGCACGGCTAGTATCTCCTTGGTTGGGTGGAACTACACCTCGTTCTGGTTCTGGTGCTTCTCCGTGGGATCACATATCTCCATCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCACCTCACATTTACTTCTGAAAATTCGCAATCCTTCCAGGGTGGAGAAGCAGTTAATTCTGATTTGGCTGGAGAAAACAATTATGAAATTAGTGAAAGCATGCATGCAGAGATGGAATACAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGCAATACAATGTATGATACGAGTGATAGCTCATCACTGTTTTTCGGGGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACAAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCCCAAATGACTGCTGATAACGCGCAGTGGGAGGATCGCCAACTTTTGAGATCCGGTGCTGTTAGAGGTACTGAGGTGCAGACGGAATTTGACGATGAAGACGAACGCAAAGTTATTCTTCTTGTACACGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCAATAAAAGATCCTACGTCTGATATGGCCATAATTTCACGCAAAGGGTCTACTTTGGTTAGGGAAATTCATGAGAAGCAAAGTTCTAACAAATCACGACAACGTTTTTGGGAGCTTGCAGGTTCAAAACTTGGGGATATCCTTGGTGTTGAGAAAACAGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGATGCTAAGTTTGCCCAACATATGAAGAATGATCAAGCTGTCAGTGATTTTGCAATGTCAAAAACCCTCGCACAGCAACGACAGTATTTACCCATATATTCTGTGCGGGATGAGTTGTTGCAGGTAATTCGAGAAAACCAAGTTATCGTTGTTGTCGGAGAAACTGGTTCTGGAAAAACAACTCAGCTGACACAGTATCTGTATGAAGATGGATACACAGTAGGTGGTATAGTAGGATGCACACAACCGAGGCGTGTGGCAGCCATGAGTGTTGCCAAAAGAGTTAGTGAGGAAATGGAGACTGAACTTGGTGATAAAGTTGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTATATGACTGATGGAGTTCTTTTGCGCGAAACGCTAAGGGATTCTGACCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTATTTGGGATTTTGAAAAAAGTGGTTGCCCAACGACGTGATTTCAAGCTGATTGTGACATCTGCAACTCTAAATGCGCAGAAGTTTTCAAATTTCTTTGGAAGTGTACCGATCTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCCTGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGTGATATCCTCATCTTCATGACTGGCCAAGATGAGATTGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTATCTCCTCCTCTAATAAAGCGGTGCCAAAACTGTTGATACTTCCTATATATTCACAGTTGCCTGCTGACCTGCAAGCGAAGATATTCCAAAAAGCGGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCAGAGACCTCTCTGACTGTCGATGGAATCTTTTACGTCATCGACACTGGTTATGGAAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGGGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACAGAGAATGCATACCTGAATGAAATGCTCCCAAGTCCTGTGCCAGAAATCCAGAGGACTAACCTTGGTAACGTGGTGTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGATCCACCTCCCCAAGACAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTAGGTGCTCTTAACAATGTGGGGGGCTTAACAGATCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTCTTGATGGGTGCTGAGCTGGGATGCTTAGATGAGGTTTTGACAATTGTGTCCATGCTGTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGTGATGCGGCACGAGAGAAATTCTCTATTCCTGAATCTGATCACTTGACACTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGTGACCATTACTTGCATGTTAAAGGGTTGCGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAGATCCCACTGACGACTTGTTGGCCTGATACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGAGAGTATGTTAATTCTAGAACGGGGATGCCATGCCATCTACACCCGAGCAGTGCTCTTTATGGTATGGGATGCACTCCAGACTATGTCGTTTATCATGAACTGATTTTGACTACCAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTGGGACCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTATTGGAGCATAAGAAGAGACAGAAGGAAGAGAAGACAGCTATGGAGCAAGAAATGGAGAATCTGAGAAAGGCTCAAGCAGAGGAGGAGATAGAAAACAAGCAGAAGGAGAAACAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTCTGAGCAAGCGCTCTTCCACATATTTAAGGCCGAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1308

Amino Acids

147.52

Weight (kDa)

6.02

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 617 - 767 5.5e-09 DEAD/DEAH box helicase
Helicase_C PF00271 814 - 943 9.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1008 - 1033 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1034 - 1093 1.7e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1151 - 1228 4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AasI GACNNNNNNGTC 1 cut(s) 3169
Acc16I TGCGCA 1 cut(s) 2298
Acc36I ACCTGC 4 cut(s) 1625, 1672, 1849, 2610
AccB1I GGYRCC 1 cut(s) 2554
AccBSI CCGCTC 2 cut(s) 130, 2518
AccI GTMKAC 2 cut(s) 1565, 2736
AccII CGCG 2 cut(s) 1346, 2136
AccIII TCCGGA 1 cut(s) 124
AclI AACGTT 3 cut(s) 433, 1618, 3285
AcoI YGGCCR 5 cut(s) 673, 748, 1477, 1542, 2476
AcsI RAATTY 7 cut(s) 492, 1048, 1409, 1581, 2311, 3245, 3858
AcuI CTGAAG 2 cut(s) 42, 3431
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 9 cut(s) 312, 376, 410, 1393, 1449, 2328, 2375, 2852, 3632
AfeI AGCGCT 1 cut(s) 3884
AfiI CCNNNNNNNGG 7 cut(s) 896, 917, 2199, 2626, 2749, 2750, 3213
AflII CTTAAG 1 cut(s) 2948
AflIII ACRYGT 1 cut(s) 2837
AjiI CACGTC 3 cut(s) 800, 2218, 2258
AjnI CCWGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
AjuI GAANNNNNNNTTGG 2 cut(s) 2988, 3020
AleI CACNNNNGTG 2 cut(s) 2216, 3650
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 2 cut(s) 3043, 3565
Alw26I GTCTC 7 cut(s) 420, 520, 782, 2030, 2208, 2666, 3730
AlwNI CAGNNNCTG 2 cut(s) 1697, 1936
Ama87I CYCGRG 1 cut(s) 3553
Aor13HI TCCGGA 1 cut(s) 124
Aor51HI AGCGCT 1 cut(s) 3884
ApeKI GCWGC 9 cut(s) 72, 1997, 2495, 2789, 2792, 3388, 3847, 3850, 3853
ApoI RAATTY 7 cut(s) 492, 1048, 1409, 1581, 2311, 3245, 3858
ArsI GACNNNNNNTTYG 6 cut(s) 433, 465, 1605, 1637, 2207, 2239
Asp700I GAANNNNTTC 3 cut(s) 435, 2615, 2961
AspLEI GCGC 4 cut(s) 1348, 2136, 2299, 3885
AspS9I GGNCC 8 cut(s) 843, 861, 1020, 2090, 2828, 3100, 3204, 3680
AsuII TTCGAA 1 cut(s) 1279
AvaI CYCGRG 1 cut(s) 3553
AvaII GGWCC 5 cut(s) 843, 1020, 2828, 3100, 3680
BaeI ACNNNNGTAYC 8 cut(s) 715, 748, 1383, 1416, 3296, 3329, 3432, 3465
BalI TGGCCA 2 cut(s) 1544, 2478
BamHI GGATCC 1 cut(s) 2983
BanI GGYRCC 1 cut(s) 2554
BanII GRGCYC 1 cut(s) 2641
BauI CACGAG 1 cut(s) 3237
BbsI GAAGAC 3 cut(s) 1428, 3410, 3758
Bbv12I GWGCWC 2 cut(s) 3043, 3565
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 9 cut(s) 84, 2009, 2482, 2776, 2779, 3375, 3859, 3862, 3865
BceAI ACGGC 2 cut(s) 63, 893
BcgI CGANNNNNNTGC 2 cut(s) 1847, 1881
BciT130I CCWGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
BciVI GTATCC 2 cut(s) 974, 1943
BclI TGATCA 2 cut(s) 1768, 3265
BcoDI GTCTC 7 cut(s) 420, 520, 782, 2030, 2208, 2666, 3730
BfaI CTAG 8 cut(s) 242, 462, 881, 2745, 3369, 3392, 3492, 3525
BfmI CTRYAG 1 cut(s) 3648
BfoI RGCGCY 1 cut(s) 3886
BfrI CTTAAG 1 cut(s) 2948
BfuAI ACCTGC 4 cut(s) 1625, 1672, 1849, 2610
BfuI GTATCC 2 cut(s) 974, 1943
BglII AGATCT 3 cut(s) 992, 3067, 3396
BlpI GCTNAGC 1 cut(s) 3135
Bme1390I CCNGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
Bme18I GGWCC 5 cut(s) 843, 1020, 2828, 3100, 3680
BmeT110I CYCGRG 1 cut(s) 3553
BmgBI CACGTC 3 cut(s) 800, 2218, 2258
BmgT120I GGNCC 8 cut(s) 843, 861, 1020, 2090, 2828, 3100, 3204, 3680
BmrFI CCNGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
BmrI ACTGGG 1 cut(s) 2097
BmuI ACTGGG 1 cut(s) 2097
BpiI GAAGAC 3 cut(s) 1428, 3410, 3758
BplI GAGNNNNNCTC 8 cut(s) 1291, 1323, 3233, 3265, 3547, 3579, 3775, 3807
BpmI CTGGAG 4 cut(s) 942, 1116, 2433, 3570
Bpu10I CCTNAGC 2 cut(s) 189, 2144
Bpu1102I GCTNAGC 1 cut(s) 3135
Bpu14I TTCGAA 1 cut(s) 1279
BpuEI CTTGAG 2 cut(s) 2924, 3777
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 1 cut(s) 985
BsaI GGTCTC 2 cut(s) 2208, 2666
BsaWI WCCGGW 2 cut(s) 124, 1376
Bsc4I CCNNNNNNNGG 7 cut(s) 896, 917, 2199, 2626, 2749, 2750, 3213
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 9 cut(s) 310, 819, 959, 1906, 2092, 2479, 2719, 2830, 3329
Bse3DI GCAATG 1 cut(s) 1797
Bse8I GATNNNNATC 1 cut(s) 985
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
BseJI GATNNNNATC 1 cut(s) 985
BseLI CCNNNNNNNGG 7 cut(s) 896, 917, 2199, 2626, 2749, 2750, 3213
BseMI GCAATG 1 cut(s) 1797
BseMII CTCAG 9 cut(s) 54, 180, 255, 1322, 1386, 1934, 3126, 3773, 3866
BseNI ACTGG 9 cut(s) 310, 819, 959, 1906, 2092, 2479, 2719, 2830, 3329
BseRI GAGGAG 8 cut(s) 260, 263, 1691, 2527, 2530, 3236, 3815, 3818
BseXI GCAGC 9 cut(s) 84, 2009, 2482, 2776, 2779, 3375, 3859, 3862, 3865
BseYI CCCAGC 3 cut(s) 2807, 3139, 3384
BsgI GTGCAG 2 cut(s) 1421, 2430
Bsh1236I CGCG 2 cut(s) 1346, 2136
BshNI GGYRCC 1 cut(s) 2554
BsiHKAI GWGCWC 2 cut(s) 3043, 3565
BsiHKCI CYCGRG 1 cut(s) 3553
BsiSI CCGG 3 cut(s) 125, 751, 1377
BslFI GGGAC 6 cut(s) 569, 829, 1006, 1309, 3335, 3693
BslI CCNNNNNNNGG 7 cut(s) 896, 917, 2199, 2626, 2749, 2750, 3213
BsmAI GTCTC 7 cut(s) 420, 520, 782, 2030, 2208, 2666, 3730
BsmBI CGTCTC 1 cut(s) 520
BsmFI GGGAC 6 cut(s) 569, 829, 1006, 1309, 3335, 3693
BsmI GAATGC 1 cut(s) 2866
Bso31I GGTCTC 2 cut(s) 2208, 2666
BsoBI CYCGRG 1 cut(s) 3553
Bsp119I TTCGAA 1 cut(s) 1279
Bsp1286I GDGCHC 3 cut(s) 2641, 3043, 3565
Bsp13I TCCGGA 1 cut(s) 124
Bsp1407I TGTACA 3 cut(s) 1447, 2373, 2850
Bsp1720I GCTNAGC 1 cut(s) 3135
BspCNI CTCAG 9 cut(s) 55, 181, 256, 1321, 1387, 1933, 3127, 3774, 3867
BspEI TCCGGA 1 cut(s) 124
BspFNI CGCG 2 cut(s) 1346, 2136
BspHI TCATGA 3 cut(s) 1585, 2469, 3604
BspMAI CTGCAG 1 cut(s) 3652
BspMI ACCTGC 4 cut(s) 1625, 1672, 1849, 2610
BspQI GCTCTTC 3 cut(s) 62, 2769, 3892
BspT104I TTCGAA 1 cut(s) 1279
BspT107I GGYRCC 1 cut(s) 2554
BspTI CTTAAG 1 cut(s) 2948
BspTNI GGTCTC 2 cut(s) 2208, 2666
BsrBI CCGCTC 2 cut(s) 130, 2518
BsrDI GCAATG 1 cut(s) 1797
BsrFI RCCGGY 1 cut(s) 750
BsrGI TGTACA 3 cut(s) 1447, 2373, 2850
BsrI ACTGG 9 cut(s) 310, 819, 959, 1906, 2092, 2479, 2719, 2830, 3329
BssAI RCCGGY 1 cut(s) 750
BssNAI GTATAC 1 cut(s) 2737
BssSI CACGAG 1 cut(s) 3237
BssT1I CCWWGG 4 cut(s) 890, 1657, 2917, 3624
Bst1107I GTATAC 1 cut(s) 2737
Bst2BI CACGAG 1 cut(s) 3237
Bst2UI CCWGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
BstAFI CTTAAG 1 cut(s) 2948
BstAPI GCANNNNNTGC 1 cut(s) 1697
BstAUI TGTACA 3 cut(s) 1447, 2373, 2850
BstBAI YACGTR 1 cut(s) 971
BstBI TTCGAA 1 cut(s) 1279
BstDSI CCRYGG 2 cut(s) 45, 935
BstEII GGTNACC 1 cut(s) 3332
BstFNI CGCG 2 cut(s) 1346, 2136
BstH2I RGCGCY 1 cut(s) 3886
BstHHI GCGC 4 cut(s) 1348, 2136, 2299, 3885
BstMAI GTCTC 7 cut(s) 420, 520, 782, 2030, 2208, 2666, 3730
BstNI CCWGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
BstNSI RCATGY 5 cut(s) 1129, 1133, 2841, 3350, 3637
BstPI GGTNACC 1 cut(s) 3332
BstSCI CCNGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
BstSFI CTRYAG 1 cut(s) 3648
BstUI CGCG 2 cut(s) 1346, 2136
BstV1I GCAGC 9 cut(s) 84, 2009, 2482, 2776, 2779, 3375, 3859, 3862, 3865
BstV2I GAAGAC 3 cut(s) 1428, 3410, 3758
BstX2I RGATCY 9 cut(s) 992, 1373, 1525, 2983, 3067, 3380, 3396, 3414, 3839
BstXI CCANNNNNNTGG 2 cut(s) 2807, 3652
BstYI RGATCY 9 cut(s) 992, 1373, 1525, 2983, 3067, 3380, 3396, 3414, 3839
BstZ17I GTATAC 1 cut(s) 2737
BsuI GTATCC 2 cut(s) 974, 1943
BtgI CCRYGG 2 cut(s) 45, 935
BtgZI GCGATG 1 cut(s) 3699
BtrI CACGTC 3 cut(s) 800, 2218, 2258
BtsI GCAGTG 6 cut(s) 1355, 3302, 3565, 3644, 3645, 3657
BveI ACCTGC 4 cut(s) 1625, 1672, 1849, 2610
CaiI CAGNNNCTG 2 cut(s) 1697, 1936
CciI TCATGA 3 cut(s) 1585, 2469, 3604
CfoI GCGC 4 cut(s) 1348, 2136, 2299, 3885
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 8 cut(s) 843, 861, 1020, 2090, 2828, 3100, 3204, 3680
CseI GACGC 1 cut(s) 963
CsiI ACCWGGT 1 cut(s) 1009
Csp6I GTAC 9 cut(s) 311, 375, 409, 1392, 1448, 2327, 2374, 2851, 3631
CspCI CAANNNNNGTGG 2 cut(s) 3648, 3683
CviQI GTAC 9 cut(s) 311, 375, 409, 1392, 1448, 2327, 2374, 2851, 3631
DraIII CACNNNGTG 1 cut(s) 800
DrdI GACNNNNNNGTC 1 cut(s) 3169
DseDI GACNNNNNNGTC 1 cut(s) 3169
EaeI YGGCCR 5 cut(s) 673, 748, 1477, 1542, 2476
EciI GGCGGA 1 cut(s) 3686
Eco130I CCWWGG 4 cut(s) 890, 1657, 2917, 3624
Eco147I AGGCCT 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 2641
Eco31I GGTCTC 2 cut(s) 2208, 2666
Eco32I GATATC 3 cut(s) 335, 1654, 2458
Eco47I GGWCC 5 cut(s) 843, 1020, 2828, 3100, 3680
Eco47III AGCGCT 1 cut(s) 3884
Eco57I CTGAAG 2 cut(s) 42, 3431
Eco88I CYCGRG 1 cut(s) 3553
Eco91I GGTNACC 1 cut(s) 3332
EcoO109I RGGNCCY 3 cut(s) 861, 1020, 3680
EcoO65I GGTNACC 1 cut(s) 3332
EcoRII CCWGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
EcoRV GATATC 3 cut(s) 335, 1654, 2458
EcoT14I CCWWGG 4 cut(s) 890, 1657, 2917, 3624
EcoT22I ATGCAT 2 cut(s) 1131, 2866
EcoT38I GRGCYC 1 cut(s) 2641
ErhI CCWWGG 4 cut(s) 890, 1657, 2917, 3624
Esp3I CGTCTC 1 cut(s) 520
FalI AAGNNNNNCTT 2 cut(s) 1425, 1457
FaqI GGGAC 6 cut(s) 569, 829, 1006, 1309, 3335, 3693
FauI CCCGC 5 cut(s) 71, 708, 1836, 2805, 2809
FauNDI CATATG 1 cut(s) 1758
FbaI TGATCA 2 cut(s) 1768, 3265
FblI GTMKAC 2 cut(s) 1565, 2736
FriOI GRGCYC 1 cut(s) 2641
FspBI CTAG 8 cut(s) 242, 462, 881, 2745, 3369, 3392, 3492, 3525
FspI TGCGCA 1 cut(s) 2298
GlaI GCGC 4 cut(s) 1347, 2135, 2298, 3884
GsaI CCCAGC 3 cut(s) 2811, 3143, 3388
GsuI CTGGAG 4 cut(s) 942, 1116, 2433, 3570
HaeII RGCGCY 1 cut(s) 3886
HapII CCGG 3 cut(s) 125, 751, 1377
HgaI GACGC 1 cut(s) 963
HhaI GCGC 4 cut(s) 1348, 2136, 2299, 3885
Hin6I GCGC 4 cut(s) 1346, 2134, 2297, 3883
HinP1I GCGC 4 cut(s) 1346, 2134, 2297, 3883
HinfI GANTC 8 cut(s) 179, 198, 266, 2150, 2694, 3260, 3704, 3778
HpaII CCGG 3 cut(s) 125, 751, 1377
Hpy166II GTNNAC 8 cut(s) 311, 431, 1450, 1566, 2327, 2737, 2853, 3289
Hpy8I GTNNAC 8 cut(s) 311, 431, 1450, 1566, 2327, 2737, 2853, 3289
Hpy99I CGWCG 2 cut(s) 801, 2259
HspAI GCGC 4 cut(s) 1346, 2134, 2297, 3883
KflI GGGWCCC 1 cut(s) 3680
Kpn2I TCCGGA 1 cut(s) 124
Ksp22I TGATCA 2 cut(s) 1768, 3265
LguI GCTCTTC 3 cut(s) 62, 2769, 3892
LmnI GCTCC 7 cut(s) 127, 1627, 1678, 2886, 3655, 3724, 3763
Lsp1109I GCAGC 9 cut(s) 84, 2009, 2482, 2776, 2779, 3375, 3859, 3862, 3865
MabI ACCWGGT 1 cut(s) 1009
MaeI CTAG 8 cut(s) 242, 462, 881, 2745, 3369, 3392, 3492, 3525
MbiI CCGCTC 2 cut(s) 130, 2518
MfeI CAATTG 1 cut(s) 3165
MflI RGATCY 9 cut(s) 992, 1373, 1525, 2983, 3067, 3380, 3396, 3414, 3839
MhlI GDGCHC 3 cut(s) 2641, 3043, 3565
MlsI TGGCCA 2 cut(s) 1544, 2478
MluNI TGGCCA 2 cut(s) 1544, 2478
MlyI GAGTC 2 cut(s) 188, 275
MmeI TCCRAC 2 cut(s) 590, 1873
Mox20I TGGCCA 2 cut(s) 1544, 2478
Mph1103I ATGCAT 2 cut(s) 1131, 2866
MroI TCCGGA 1 cut(s) 124
MroXI GAANNNNTTC 3 cut(s) 435, 2615, 2961
MscI TGGCCA 2 cut(s) 1544, 2478
MseI TTAA 9 cut(s) 30, 1080, 2364, 2949, 3045, 3062, 3351, 3519, 3899
MslI CAYNNNNRTG 4 cut(s) 567, 2216, 2468, 3650
Msp20I TGGCCA 2 cut(s) 1544, 2478
MspA1I CMGCKG 3 cut(s) 75, 1924, 3388
MspCI CTTAAG 1 cut(s) 2948
MspI CCGG 3 cut(s) 125, 751, 1377
MspR9I CCNGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
MunI CAATTG 1 cut(s) 3165
Mva1269I GAATGC 1 cut(s) 2866
MvaI CCWGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
MvnI CGCG 2 cut(s) 1346, 2136
NdeI CATATG 1 cut(s) 1758
NmeAIII GCCGAG 1 cut(s) 1505
NmuCI GTSAC 5 cut(s) 317, 486, 2275, 2943, 3332
NsbI TGCGCA 1 cut(s) 2298
NsiI ATGCAT 2 cut(s) 1131, 2866
NspI RCATGY 5 cut(s) 1129, 1133, 2841, 3350, 3637
NspV TTCGAA 1 cut(s) 1279
OliI CACNNNNGTG 2 cut(s) 2216, 3650
PaeI GCATGC 2 cut(s) 1129, 1133
PagI TCATGA 3 cut(s) 1585, 2469, 3604
PceI AGGCCT 1 cut(s) 121
PciI ACATGT 1 cut(s) 2837
PciSI GCTCTTC 3 cut(s) 62, 2769, 3892
PcsI WCGNNNNNNNCGW 1 cut(s) 1422
PctI GAATGC 1 cut(s) 2866
PdmI GAANNNNTTC 3 cut(s) 435, 2615, 2961
PfeI GAWTC 6 cut(s) 198, 2150, 2694, 3260, 3704, 3778
PflFI GACNNNGTC 1 cut(s) 3593
PfoI TCCNGGA 2 cut(s) 1470, 2830
PleI GAGTC 2 cut(s) 187, 274
PpsI GAGTC 2 cut(s) 187, 274
Ppu21I YACGTR 1 cut(s) 971
PpuMI RGGWCCY 2 cut(s) 1020, 3680
PscI ACATGT 1 cut(s) 2837
PsiI TTATAA 1 cut(s) 651
Psp1406I AACGTT 3 cut(s) 433, 1618, 3285
Psp5II RGGWCCY 2 cut(s) 1020, 3680
Psp6I CCWGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
PspEI GGTNACC 1 cut(s) 3332
PspFI CCCAGC 3 cut(s) 2807, 3139, 3384
PspGI CCWGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
PspPI GGNCC 8 cut(s) 843, 861, 1020, 2090, 2828, 3100, 3204, 3680
PspPPI RGGWCCY 2 cut(s) 1020, 3680
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PstI CTGCAG 1 cut(s) 3652
PstNI CAGNNNCTG 2 cut(s) 1697, 1936
PsuI RGATCY 9 cut(s) 992, 1373, 1525, 2983, 3067, 3380, 3396, 3414, 3839
PsyI GACNNNGTC 1 cut(s) 3593
PvuII CAGCTG 2 cut(s) 1924, 3388
RsaI GTAC 9 cut(s) 312, 376, 410, 1393, 1449, 2328, 2375, 2852, 3632
RsaNI GTAC 9 cut(s) 311, 375, 409, 1392, 1448, 2327, 2374, 2851, 3631
RseI CAYNNNNRTG 4 cut(s) 567, 2216, 2468, 3650
SapI GCTCTTC 3 cut(s) 62, 2769, 3892
SaqAI TTAA 9 cut(s) 30, 1080, 2364, 2949, 3045, 3062, 3351, 3519, 3899
Sau96I GGNCC 8 cut(s) 843, 861, 1020, 2090, 2828, 3100, 3204, 3680
SchI GAGTC 2 cut(s) 188, 275
ScrFI CCNGG 9 cut(s) 96, 747, 1011, 1064, 1472, 2346, 2451, 2832, 3870
SduI GDGCHC 3 cut(s) 2641, 3043, 3565
SexAI ACCWGGT 1 cut(s) 1009
SfcI CTRYAG 1 cut(s) 3648
SfuI TTCGAA 1 cut(s) 1279
SinI GGWCC 5 cut(s) 843, 1020, 2828, 3100, 3680
SmiMI CAYNNNNRTG 4 cut(s) 567, 2216, 2468, 3650
SmlI CTYRAG 3 cut(s) 2939, 2948, 3792
SmoI CTYRAG 3 cut(s) 2939, 2948, 3792
SphI GCATGC 2 cut(s) 1129, 1133
SseBI AGGCCT 1 cut(s) 121
SspI AATATT 2 cut(s) 2665, 3004
SspMI CTAG 8 cut(s) 242, 462, 881, 2745, 3369, 3392, 3492, 3525
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 9 cut(s) 94, 745, 1009, 1062, 1470, 2344, 2449, 2830, 3868
StyI CCWWGG 4 cut(s) 890, 1657, 2917, 3624
TaqI TCGA 8 cut(s) 106, 364, 658, 1279, 1867, 2688, 2709, 3316
TatI WGTACW 4 cut(s) 1447, 2373, 2850, 3630
TauI GCSGC 2 cut(s) 78, 3236
TfiI GAWTC 6 cut(s) 198, 2150, 2694, 3260, 3704, 3778
Tru1I TTAA 9 cut(s) 30, 1080, 2364, 2949, 3045, 3062, 3351, 3519, 3899
Tru9I TTAA 9 cut(s) 30, 1080, 2364, 2949, 3045, 3062, 3351, 3519, 3899
TseFI GTSAC 5 cut(s) 317, 486, 2275, 2943, 3332
TseI GCWGC 9 cut(s) 72, 1997, 2495, 2789, 2792, 3388, 3847, 3850, 3853
Tsp45I GTSAC 5 cut(s) 317, 486, 2275, 2943, 3332
TspGWI ACGGA 2 cut(s) 924, 1421
Tth111I GACNNNGTC 1 cut(s) 3593
Vha464I CTTAAG 1 cut(s) 2948
VpaK11BI GGWCC 5 cut(s) 843, 1020, 2828, 3100, 3680
XapI RAATTY 7 cut(s) 492, 1048, 1409, 1581, 2311, 3245, 3858
XbaI TCTAGA 2 cut(s) 241, 3524
XceI RCATGY 5 cut(s) 1129, 1133, 2841, 3350, 3637
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmiI GTMKAC 2 cut(s) 1565, 2736
XmnI GAANNNNTTC 3 cut(s) 435, 2615, 2961
XspI CTAG 8 cut(s) 242, 462, 881, 2745, 3369, 3392, 3492, 3525
Zsp2I ATGCAT 2 cut(s) 1131, 2866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.