RH4BG308200

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0002001
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG308200.1

Sequence Viewer

Length: 3921 bp
ATGGAGAATGGAGGAAACAGTGAAGCTATTAAGCTGGACAAGACGACGACGACATTGGAACCCGAGAATAGCAGTGGTGGGGGATTATTTGTTCCTGGGAAAGATCGAGTTGTGTATAGACCTCCCGAGAGGAAATCTCTCTTAGGTTTAGATGTCCTTGCCATTGCAAAACGGGAAGAGTCTAAGGTTGAGGGTGGATTCAAAACCCCAAGAGAAAGAGTTGCATCGGTTGTCGCATCTCTAGAGGAGGAGGAAAACTCCGAGTCTGGTGTAATAGATGAAACAGGGAGTGATATATCCACTGGTGTACGCAGTCATTCCAAGAGGCGATATCGTGGAATATCTGCAAGTGATACACCTCGGACAGAAAGTACGGTAACGGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGTGAACGTTTTCGGACAGATGTTTTGGCCACTCCTAGTGGAAGTTCCCACAGTGTTAGGTCTCAGAGTTCATATAATAGGGATGATCGTGGTAGTGACAGAAGAGATCACAAGGGTAGGTCTGACAGGGACGACAATGATAGTGAGAGAAGAGATTACCAAGATAGTAGTAGGGGCGATAGTTGGAGGGAGAGAAAAAGATATGGCAGTGATAGTAAGGATTATTATGGTCGAAGAGAAGAACGTGGTCGATATGAACAAGAATATGGTGGAGAATACGAAAGAAAGCGGGGTAGATACGAAGGTTCAAAGAGGACACCCGGCCGCTCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGACACCACGACGTGATGGTTACTCTAATACCAGTAGGCGTCATCAACCATCACGATCCCCTATGCTACTCGGGGCCTCACCTGATGCACGACTAGTATCTCCTTGGTTGGGTGGGAATACACCTCATTCTGGTTCTGCTGCTTCACCATGGGATCACATATCTCCATCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCAGCTCACATTTTCTTCGGAAAATTCACAATCATTCCAGGGTGGAGAAGCATTTAATACTGATTCGGCTGGAGAACACAATTATGAAATTAGTGAAAGCATGCGTGCAGAGATGGAATACAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGAAATACAATGTATGATACGAGTGATAGCTCATCATTGTTTTTCGGAGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCTCAGATGACTGCTGATAATGCGCAGTGGGAGGATCGCCAACTTTTGAGATCGGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAATTTGATGATGAAGACGAACGCAAAGTTATTCTTCTTGTACATGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCAATAAAGGATCCTACATCTGATATGGCCATAATATCACGCAAAGGGTCTACTTTGGTTAGGGAAATCCATGAGAAGCAAAGTTCTAACAAATCACGCCAACGTTTTTGGGAGCTTGCTGGTTCCAAACTTGGGGATATCCTTGGTGTTGAGAAAACTGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGACGCTAAGTTTGCCCAACATATGAAGAATGGGGAAGCTGTCAGTGATTTTGCAAAGTCAAAAACCCTCTCACAGCAACGGCAGTATTTGCCCATATATTCTGTGAGGGATGATTTGTTGCAGGTGATTCGAGAAAATCAAGTTATCATTGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAGTATCTGCATGAAGATGGATACACGGTAAATGGTATAGTAGGTTGCACTCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAGGAAATGGAGACCGAACTTGGTGATAAAGTCGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTTCTTTTGCGCGAAACTCTAAGGGATTCTGATCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTGTTTGGAATTTTAAAAAAAGTGGTTGCCCAACGTCGTGATTTCAAGCTCATTGTGACCTCTGCAACTCTAAATGCTCAAAAGTTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCATGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGTGACATCCTCATCTTCATGACTGGCCAAGATGAGATTGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTACCTCCTCCTCTAATAAAGCGGTGCCAAAACTGTTGATACTTCCTATATATTCACAGCTGCCTGCTGACCTGCAAGCGAAGATTTTCCAAAGAGCTGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCGGAGACGTCTCTGACTGTCGATGGAATCTTTTACGTCATTGACACAGGTTATGGCAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGTGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACGGAGAATGCATACCTGAATGAAATGCTCCCAAGTCCTGTGCCGGAAATCCAGAGGACTAACCTTGGTAATGTGGTCTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGATCCACCTCCCCAAGATAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTGGGTGCTCTTAACAACGTGGGCGGCTTGACAGACCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTTTTGATGGGTGCTGAGCTGGGATGCTTGGACGAGGTTTTGACAATTGTGTCCATGCTCTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATCGGGCAGAGGAGAGTGATGCTGCACGAGAGAAATTCTCTATTCCTGAATCCGATCACTTGACGTTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCCCTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGACTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTTGTGTATCATGAACTGATTTTGACTACAAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTGGGGCCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTGTTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACAGCTATGGAGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAAGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAGATTTCAATGCCTGGTTTGAACAAGCGCTCTTCTACATATTTGAGGCCAAAGAAACTCGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1306

Amino Acids

147.29

Weight (kDa)

6.07

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 618 - 766 1.6e-08 DEAD/DEAH box helicase
Helicase_C PF00271 813 - 942 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1007 - 1032 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1033 - 1092 1.7e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1150 - 1227 4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1846
AasI GACNNNNNNGTC 1 cut(s) 3166
AatII GACGTC 1 cut(s) 2675
Acc16I TGCGCA 1 cut(s) 1344
Acc36I ACCTGC 3 cut(s) 1669, 1846, 2607
AccB1I GGYRCC 1 cut(s) 2551
AccBSI CCGCTC 2 cut(s) 750, 2515
AccI GTMKAC 2 cut(s) 1562, 2733
AccII CGCG 1 cut(s) 2133
AciI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
AclI AACGTT 3 cut(s) 430, 1615, 3282
AclWI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
AcoI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
AcsI RAATTY 5 cut(s) 1045, 1406, 2226, 2308, 3242
AcuI CTGAAG 2 cut(s) 2646, 3515
AcyI GRCGYC 2 cut(s) 823, 2672
AdeI CACNNNGTG 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 3881
AfiI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
AflII CTTAAG 1 cut(s) 2945
AflIII ACRYGT 1 cut(s) 2834
AhlI ACTAGT 1 cut(s) 877
AjiI CACGTC 2 cut(s) 797, 2215
AjnI CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
AjuI GAANNNNNNNTTGG 2 cut(s) 2985, 3017
AleI CACNNNNGTG 2 cut(s) 2213, 3647
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 2 cut(s) 3040, 3562
Alw26I GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
AlwI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
AlwNI CAGNNNCTG 2 cut(s) 1694, 1933
Ama87I CYCGRG 3 cut(s) 62, 125, 854
Aor51HI AGCGCT 1 cut(s) 3881
ApaI GGGCCC 1 cut(s) 3681
ApoI RAATTY 5 cut(s) 1045, 1406, 2226, 2308, 3242
ArsI GACNNNNNNTTYG 6 cut(s) 430, 462, 1727, 1759, 2204, 2236
Asp700I GAANNNNTTC 3 cut(s) 432, 2612, 2958
AspLEI GCGC 3 cut(s) 1345, 2133, 3882
AspS9I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 9 cut(s) 437, 855, 921, 1718, 1870, 2057, 2428, 2462, 3385
AsuII TTCGAA 1 cut(s) 1276
AvaI CYCGRG 3 cut(s) 62, 125, 854
AvaII GGWCC 4 cut(s) 1017, 2825, 3097, 3331
BaeGI GKGCMC 1 cut(s) 3681
BaeI ACNNNNGTAYC 6 cut(s) 712, 745, 1380, 1413, 3293, 3326
BalI TGGCCA 3 cut(s) 452, 1541, 2475
BamHI GGATCC 2 cut(s) 1522, 2980
BanI GGYRCC 1 cut(s) 2551
BanII GRGCYC 2 cut(s) 2638, 3681
BauI CACGAG 1 cut(s) 3234
BbsI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
Bbv12I GWGCWC 2 cut(s) 3040, 3562
BceAI ACGGC 1 cut(s) 1829
BcgI CGANNNNNNTGC 2 cut(s) 1844, 1878
BciT130I CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BciVI GTATCC 2 cut(s) 971, 1940
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BcuI ACTAGT 1 cut(s) 877
BfaI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
BfmI CTRYAG 1 cut(s) 3645
BfoI RGCGCY 1 cut(s) 3883
BfrI CTTAAG 1 cut(s) 2945
BfuAI ACCTGC 3 cut(s) 1669, 1846, 2607
BfuI GTATCC 2 cut(s) 971, 1940
BglII AGATCT 2 cut(s) 989, 3393
BlpI GCTNAGC 1 cut(s) 3132
Bme1390I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bme18I GGWCC 4 cut(s) 1017, 2825, 3097, 3331
BmeT110I CYCGRG 3 cut(s) 62, 125, 854
BmgBI CACGTC 2 cut(s) 797, 2215
BmgT120I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
BmrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BmrI ACTGGG 3 cut(s) 766, 2094, 3537
BmuI ACTGGG 3 cut(s) 766, 2094, 3537
BpiI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BpmI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
Bpu1102I GCTNAGC 1 cut(s) 3132
Bpu14I TTCGAA 1 cut(s) 1276
BpuEI CTTGAG 2 cut(s) 2921, 3774
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 2 cut(s) 982, 2151
BsaHI GRCGYC 2 cut(s) 823, 2672
BsaI GGTCTC 3 cut(s) 489, 2027, 2205
BsaXI ACNNNNNCTCC 6 cut(s) 1444, 1474, 2428, 2458, 3644, 3674
Bsc4I CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 2 cut(s) 982, 2151
BseBI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BseJI GATNNNNATC 2 cut(s) 982, 2151
BseLI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 8 cut(s) 500, 1319, 1337, 1383, 1662, 3123, 3192, 3770
BseRI GAGGAG 7 cut(s) 260, 263, 797, 1688, 2524, 2527, 3233
BseSI GKGCMC 1 cut(s) 3681
BseX3I CGGCCG 1 cut(s) 745
BseYI CCCAGC 3 cut(s) 2804, 3136, 3381
BsgI GTGCAG 4 cut(s) 1149, 1418, 2427, 3216
Bsh1236I CGCG 1 cut(s) 2133
Bsh1285I CGRYCG 1 cut(s) 748
BshNI GGYRCC 1 cut(s) 2551
BsiEI CGRYCG 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 3040, 3562
BsiHKCI CYCGRG 3 cut(s) 62, 125, 854
BsiSI CCGG 2 cut(s) 744, 2894
BslFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BslI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BsmAI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BsmBI CGTCTC 2 cut(s) 2663, 2679
BsmFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BsmI GAATGC 1 cut(s) 2863
Bso31I GGTCTC 3 cut(s) 489, 2027, 2205
BsoBI CYCGRG 3 cut(s) 62, 125, 854
Bsp119I TTCGAA 1 cut(s) 1276
Bsp120I GGGCCC 1 cut(s) 3677
Bsp1286I GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
Bsp1407I TGTACA 3 cut(s) 1444, 2370, 2847
Bsp1720I GCTNAGC 1 cut(s) 3132
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
BspCNI CTCAG 8 cut(s) 499, 1318, 1336, 1384, 1663, 3124, 3191, 3771
BspFNI CGCG 1 cut(s) 2133
BspHI TCATGA 2 cut(s) 2466, 3601
BspMAI CTGCAG 1 cut(s) 3649
BspMI ACCTGC 3 cut(s) 1669, 1846, 2607
BspPI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
BspQI GCTCTTC 2 cut(s) 2766, 3889
BspT104I TTCGAA 1 cut(s) 1276
BspT107I GGYRCC 1 cut(s) 2551
BspTI CTTAAG 1 cut(s) 2945
BspTNI GGTCTC 3 cut(s) 489, 2027, 2205
BsrBI CCGCTC 2 cut(s) 750, 2515
BsrDI GCAATG 1 cut(s) 162
BsrGI TGTACA 3 cut(s) 1444, 2370, 2847
BssNAI GTATAC 1 cut(s) 2734
BssNI GRCGYC 2 cut(s) 823, 2672
BssSI CACGAG 1 cut(s) 3234
BssT1I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Bst1107I GTATAC 1 cut(s) 2734
Bst2BI CACGAG 1 cut(s) 3234
Bst2UI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bst6I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
BstACI GRCGYC 2 cut(s) 823, 2672
BstAFI CTTAAG 1 cut(s) 2945
BstAPI GCANNNNNTGC 2 cut(s) 1694, 1822
BstAUI TGTACA 3 cut(s) 1444, 2370, 2847
BstBAI YACGTR 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 1276
BstDSI CCRYGG 1 cut(s) 932
BstFNI CGCG 1 cut(s) 2133
BstH2I RGCGCY 1 cut(s) 3883
BstHHI GCGC 3 cut(s) 1345, 2133, 3882
BstMAI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BstMCI CGRYCG 1 cut(s) 748
BstNI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BstNSI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
BstSCI CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
BstSFI CTRYAG 1 cut(s) 3645
BstSLI GKGCMC 1 cut(s) 3681
BstUI CGCG 1 cut(s) 2133
BstV2I GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BstX2I RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
BstXI CCANNNNNNTGG 2 cut(s) 2804, 3649
BstYI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
BstZ17I GTATAC 1 cut(s) 2734
BstZI CGGCCG 1 cut(s) 745
BsuI GTATCC 2 cut(s) 971, 1940
BtgI CCRYGG 1 cut(s) 932
BtgZI GCGATG 1 cut(s) 3696
BtrI CACGTC 2 cut(s) 797, 2215
BtsI GCAGTG 8 cut(s) 79, 637, 1352, 3299, 3562, 3641, 3642, 3654
BveI ACCTGC 3 cut(s) 1669, 1846, 2607
CaiI CAGNNNCTG 2 cut(s) 1694, 1933
CciI TCATGA 2 cut(s) 2466, 3601
CfoI GCGC 3 cut(s) 1345, 2133, 3882
Cfr13I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
CseI GACGC 3 cut(s) 812, 960, 1745
CsiI ACCWGGT 1 cut(s) 1006
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 3645, 3680
DraI TTTAAA 1 cut(s) 2232
DraIII CACNNNGTG 1 cut(s) 797
DrdI GACNNNNNNGTC 1 cut(s) 3166
DseDI GACNNNNNNGTC 1 cut(s) 3166
EaeI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
EagI CGGCCG 1 cut(s) 745
Eam1104I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EarI CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EciI GGCGGA 1 cut(s) 3683
EclXI CGGCCG 1 cut(s) 745
Eco130I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Eco24I GRGCYC 2 cut(s) 2638, 3681
Eco31I GGTCTC 3 cut(s) 489, 2027, 2205
Eco32I GATATC 2 cut(s) 332, 1651
Eco47I GGWCC 4 cut(s) 1017, 2825, 3097, 3331
Eco47III AGCGCT 1 cut(s) 3881
Eco52I CGGCCG 1 cut(s) 745
Eco57I CTGAAG 2 cut(s) 2646, 3515
Eco88I CYCGRG 3 cut(s) 62, 125, 854
EcoO109I RGGNCCY 3 cut(s) 858, 1017, 3677
EcoRII CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
EcoRV GATATC 2 cut(s) 332, 1651
EcoT14I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
EcoT22I ATGCAT 1 cut(s) 2863
EcoT38I GRGCYC 2 cut(s) 2638, 3681
ErhI CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Esp3I CGTCTC 2 cut(s) 2663, 2679
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 4 cut(s) 566, 1003, 3332, 3344
FauI CCCGC 2 cut(s) 705, 2806
FauNDI CATATG 1 cut(s) 1755
FblI GTMKAC 2 cut(s) 1562, 2733
FriOI GRGCYC 2 cut(s) 2638, 3681
FspBI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
FspI TGCGCA 1 cut(s) 1344
GlaI GCGC 3 cut(s) 1344, 2132, 3881
GsaI CCCAGC 3 cut(s) 2808, 3140, 3385
GsuI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
HaeII RGCGCY 1 cut(s) 3883
HapII CCGG 2 cut(s) 744, 2894
HgaI GACGC 3 cut(s) 812, 960, 1745
HhaI GCGC 3 cut(s) 1345, 2133, 3882
Hin1I GRCGYC 2 cut(s) 823, 2672
Hin6I GCGC 3 cut(s) 1343, 2131, 3880
HinP1I GCGC 3 cut(s) 1343, 2131, 3880
HpaII CCGG 2 cut(s) 744, 2894
HphI GGTGA 9 cut(s) 437, 855, 921, 1718, 1870, 2057, 2428, 2462, 3385
Hpy166II GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy8I GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy99I CGWCG 4 cut(s) 49, 52, 798, 2256
HpyAV CCTTC 8 cut(s) 719, 1169, 1240, 2396, 2969, 3490, 3691, 3814
Hsp92I GRCGYC 2 cut(s) 823, 2672
HspAI GCGC 3 cut(s) 1343, 2131, 3880
LguI GCTCTTC 2 cut(s) 2766, 3889
LmnI GCTCC 6 cut(s) 1624, 1675, 2883, 3652, 3721, 3760
MabI ACCWGGT 1 cut(s) 1006
MaeI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
MaeIII GTNAC 8 cut(s) 376, 518, 803, 2080, 2272, 2450, 2940, 3354
MbiI CCGCTC 2 cut(s) 750, 2515
MfeI CAATTG 1 cut(s) 3162
MflI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
MhlI GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
MlsI TGGCCA 3 cut(s) 452, 1541, 2475
MluNI TGGCCA 3 cut(s) 452, 1541, 2475
MlyI GAGTC 2 cut(s) 188, 272
MmeI TCCRAC 3 cut(s) 587, 1870, 3699
Mox20I TGGCCA 3 cut(s) 452, 1541, 2475
Mph1103I ATGCAT 1 cut(s) 2863
MroXI GAANNNNTTC 3 cut(s) 432, 2612, 2958
MscI TGGCCA 3 cut(s) 452, 1541, 2475
MseI TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
MslI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
Msp20I TGGCCA 3 cut(s) 452, 1541, 2475
MspA1I CMGCKG 2 cut(s) 2587, 3385
MspCI CTTAAG 1 cut(s) 2945
MspI CCGG 2 cut(s) 744, 2894
MspR9I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MunI CAATTG 1 cut(s) 3162
Mva1269I GAATGC 1 cut(s) 2863
MvaI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MvnI CGCG 1 cut(s) 2133
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 932
NdeI CATATG 1 cut(s) 1755
NmeAIII GCCGAG 2 cut(s) 1502, 3891
NmuCI GTSAC 4 cut(s) 518, 2272, 2450, 2940
NsbI TGCGCA 1 cut(s) 1344
NsiI ATGCAT 1 cut(s) 2863
NspI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
NspV TTCGAA 1 cut(s) 1276
OliI CACNNNNGTG 2 cut(s) 2213, 3647
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 2 cut(s) 2466, 3601
PaqCI CACCTGC 1 cut(s) 1846
PciI ACATGT 1 cut(s) 2834
PciSI GCTCTTC 2 cut(s) 2766, 3889
PctI GAATGC 1 cut(s) 2863
PdmI GAANNNNTTC 3 cut(s) 432, 2612, 2958
PfeI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
PfoI TCCNGGA 2 cut(s) 1467, 2827
PleI GAGTC 2 cut(s) 187, 271
PpsI GAGTC 2 cut(s) 187, 271
Ppu21I YACGTR 1 cut(s) 968
PpuMI RGGWCCY 1 cut(s) 1017
PscI ACATGT 1 cut(s) 2834
Psp1406I AACGTT 3 cut(s) 430, 1615, 3282
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspFI CCCAGC 3 cut(s) 2804, 3136, 3381
PspGI CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspOMI GGGCCC 1 cut(s) 3677
PspPI GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
PspPPI RGGWCCY 1 cut(s) 1017
PstI CTGCAG 1 cut(s) 3649
PstNI CAGNNNCTG 2 cut(s) 1694, 1933
PsuI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
PvuII CAGCTG 2 cut(s) 2587, 3385
RseI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SapI GCTCTTC 2 cut(s) 2766, 3889
SaqAI TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
Sau96I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
SchI GAGTC 2 cut(s) 188, 272
ScrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
SduI GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
SexAI ACCWGGT 1 cut(s) 1006
SfcI CTRYAG 1 cut(s) 3645
SfuI TTCGAA 1 cut(s) 1276
SinI GGWCC 4 cut(s) 1017, 2825, 3097, 3331
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SmlI CTYRAG 3 cut(s) 2936, 2945, 3789
SmoI CTYRAG 3 cut(s) 2936, 2945, 3789
SpeI ACTAGT 1 cut(s) 877
SphI GCATGC 1 cut(s) 1126
SsiI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
SspI AATATT 2 cut(s) 2662, 3001
SspMI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
StyD4I CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
StyI CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
TaqI TCGA 7 cut(s) 106, 655, 673, 1276, 1864, 2685, 3313
TaqII GACCGA 1 cut(s) 2051
TatI WGTACW 5 cut(s) 1444, 2106, 2370, 2847, 3627
TauI GCSGC 2 cut(s) 750, 3057
TfiI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
Tru1I TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
Tru9I TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
TseFI GTSAC 4 cut(s) 518, 2272, 2450, 2940
Tsp45I GTSAC 4 cut(s) 518, 2272, 2450, 2940
TspGWI ACGGA 2 cut(s) 395, 2867
Vha464I CTTAAG 1 cut(s) 2945
VpaK11BI GGWCC 4 cut(s) 1017, 2825, 3097, 3331
XapI RAATTY 5 cut(s) 1045, 1406, 2226, 2308, 3242
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
XmiI GTMKAC 2 cut(s) 1562, 2733
XmnI GAANNNNTTC 3 cut(s) 432, 2612, 2958
XspI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
ZraI GACGTC 1 cut(s) 2673
Zsp2I ATGCAT 1 cut(s) 2863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.