RchiOBHm_Chr5g0022111

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002001
Physical Location & Seq
Forward (+)
16163274 .. 16167303
4030 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30210

Sequence Viewer

Length: 3366 bp
ATGGGCCTGTACGGCAAGAGTCATGATCGCGTTGAACGAGCGATTTGGGCGTTCGTAAAATCTTCAGTTCTCCTATCAATTTCAGACCAACCCACCGATTCTCAGCTTCAGTTTTCCGATCAATTCCAGACCCAGAGCGGAGGAGGTGGAGGATTACATGTTCGTGGGACGGAGAGAGTTGTTTTCAGGCCGCCGGAGAAGAAGAAATTAGGGTTTGATGGAGGTGTCAGGAGATATAAGGGTAGGTATGATGATGGGGATGGTGGTGAAAGAAGAGATTACAGAGATGGTGGTGGTCGTAGTAGGAGTAGGGTGAGAGGAAGATATGAGGAAGATGGATATGGTGGAGATTATGGAAGAAAGAAGAGTAGATATGAAGGTTCGAAGAGGGGAGCTCAATCTGTTGCTTCTGCCTGGGATTGTATCTCTTACTCTCCGGTTCAGATACGGGCTTCTGGACAGCCAGTCAAGTCTTCGGGCTCGGGACGTGGTGGGAGTTCTCATCAGCTTGATAGTACGCTAGGAGAAGAGGGTGATTCGGTTAGATATGAGATTGATGAGAGCATGCGTTTAGAGATGGAATATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCACAGCGGTTGATACGAGTCATAGTTCGTCTTTGTTTTATGGGGATGATGCTTTTTGTCGAAAGAAGGAAACGGAGTTGGCGAAGAGACTGGTTCGGAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAGGTTGTCTCAGCACAGTGCGGATAATGCTCAGTGGGAGGATCGTCAGCTACTGCGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAACAAAGTTATGCTTCTTGTTCATGATACAAGGCCTCCTTTCCTGGATGGGAGAGTTGTTTTTACCGAGCAAGCGGAGCCAGTTATGCCAGTAAAGGATCCCACGTCTGATATGGCCATAATTTCACGCAAAGGGTCTGCTCTAGTTAGGGAAATCCATCAAAAGCAAAGTTCGAATAAATCACGTCAACGTTTTTGGGAGCTTGCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAGAAAACAGCAGAGCATATTGATGCAGATACTGCTGTAGTAGGTAAACACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCTCAGCATATGAAGAATGGGGAGGCTGTGAGTGAATTCACAAAGTCAAAAACCATTTCCGAACAACGCCAGTATCTGCCTATATATTCTGTGAGGGATGAGTTACTGCAGAAAATTCGAGAAAATCAGGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGTTACACTGTGAACGGTATAGTCGGTTGCACCCAGCCAAGACGTGTGGCAGCCATGAGTGTTGCTAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGAGAAAGTTGGCTATGCTATTCGTTTTGAGGATGTGACGGGGCCAAAAACTGTAATAAAGTACATGACTGATGGAGTACTTATGTGTGAAACACTTAGAGATTCTGACCTTGACAAGTACCGTGTAGTTGTAATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATCTTGAAAAAAGTCGTCGCCCAGCGTCGTGATTTTAAGCTCATTGTGACATCTGCTACTCTTAATGCTCAGAAATTTGCAGATTTCTTTGGAGGTGCACCTATTTTTCCCATTCCTGGAAGAACATTTCCTGTACATAAGAAGTACTTGGATACCCCATGTGAAGATTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTGGCAGCCCTCCAGGTGATATCCTCATCTTCATGACTGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTTGCAGAGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGGGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTTTACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGCTGTACACAGAGAGTGCCTACCTAAATGAAATGCTTGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTCAAGTCTCTGAAGGTTGATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCAGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAGAATGGTGGAGTTCCCATTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTAGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCGGAGTCCGATCACCTGACATTCTATAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTACAAAAGGCTAGAGAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACGTTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGTAGTGCTCTTTATGGTATGGGCTGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACGGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGACAATCTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAGCAAGCAGAGGGAGAGGGAAAAGAGGTATAAACAGCAGCAGCAGCAAGTCTCATTGGCGGGTTTGCACCAGGGTTCTTCTACATATCTGAGGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1121

Amino Acids

126.16

Weight (kDa)

6.66

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 429 - 581 2.7e-08 DEAD/DEAH box helicase
AAA_22 PF13401 437 - 570 6.5e-06 AAA domain
Helicase_C PF00271 627 - 756 1.7e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 821 - 846 9.4e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 847 - 906 2.1e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 964 - 1040 5.7e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1064
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 30
AciI CCGC 7 cut(s) 138, 191, 629, 776, 941, 3098, 3311
AclI AACGTT 1 cut(s) 1057
AclWI GGATC 5 cut(s) 804, 959, 972, 1676, 2335
AcoI YGGCCR 2 cut(s) 981, 1915
AcsI RAATTY 7 cut(s) 1223, 1302, 1742, 1869, 2595, 2684, 2853
AcuI CTGAAG 4 cut(s) 48, 92, 2408, 2861
AfiI CCNNNNNNNGG 7 cut(s) 1638, 1784, 2188, 2189, 2467, 2514, 2652
AflIII ACRYGT 3 cut(s) 157, 1429, 2276
AgsI TTSAA 8 cut(s) 35, 1171, 1675, 1844, 1931, 2207, 2851, 2921
AhdI GACNNNNNGTC 1 cut(s) 464
AjiI CACGTC 4 cut(s) 488, 972, 1052, 1430
AjnI CCWGG 7 cut(s) 413, 909, 1783, 1888, 2269, 2433, 3321
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
Alw21I GWGCWC 4 cut(s) 397, 1768, 2482, 3004
Alw26I GTCTC 7 cut(s) 703, 768, 1469, 2105, 2388, 3169, 3307
Alw44I GTGCAC 1 cut(s) 1764
AlwI GGATC 5 cut(s) 804, 959, 972, 1676, 2335
AlwNI CAGNNNCTG 4 cut(s) 808, 1136, 1264, 3055
Ama87I CYCGRG 3 cut(s) 481, 2676, 2992
ApaLI GTGCAC 1 cut(s) 1764
ApoI RAATTY 7 cut(s) 1223, 1302, 1742, 1869, 2595, 2684, 2853
Asp700I GAANNNNTTC 2 cut(s) 2442, 3051
AspA2I CCTAGG 1 cut(s) 2183
AspS9I GGNCC 9 cut(s) 4, 1529, 2267, 2422, 2539, 2643, 2820, 3119, 3345
AsuC2I CCSGG 1 cut(s) 2696
AsuHPI GGTGA 9 cut(s) 278, 325, 545, 1100, 1169, 1499, 1904, 2582, 2699
AsuII TTCGAA 2 cut(s) 383, 1040
AvaI CYCGRG 3 cut(s) 481, 2676, 2992
AvaII GGWCC 4 cut(s) 2422, 2539, 2820, 3119
AvrII CCTAGG 1 cut(s) 2183
AxyI CCTNAGG 1 cut(s) 2509
BaeGI GKGCMC 1 cut(s) 1768
BalI TGGCCA 2 cut(s) 983, 1917
BamHI GGATCC 1 cut(s) 964
BanII GRGCYC 4 cut(s) 397, 482, 2080, 2482
BbsI GAAGAC 2 cut(s) 465, 2849
Bbv12I GWGCWC 4 cut(s) 397, 1768, 2482, 3004
BceAI ACGGC 2 cut(s) 28, 3194
BcgI CGANNNNNNTGC 2 cut(s) 1286, 1320
BciT130I CCWGG 7 cut(s) 415, 911, 1785, 1890, 2271, 2435, 3323
BciVI GTATCC 3 cut(s) 1813, 2620, 3142
BcnI CCSGG 1 cut(s) 2696
BcoDI GTCTC 7 cut(s) 703, 768, 1469, 2105, 2388, 3169, 3307
BfaI CTAG 7 cut(s) 521, 1010, 2184, 2217, 2579, 2808, 2831
BfmI CTRYAG 3 cut(s) 1140, 1295, 1848
BfuAI ACCTGC 1 cut(s) 1064
BfuI GTATCC 3 cut(s) 1813, 2620, 3142
BglI GCCNNNNNGGC 1 cut(s) 12
BlnI CCTAGG 1 cut(s) 2183
BlpI GCTNAGC 1 cut(s) 1191
BmcAI AGTACT 2 cut(s) 1566, 1814
Bme1390I CCNGG 8 cut(s) 415, 911, 1785, 1890, 2271, 2435, 2696, 3323
Bme18I GGWCC 4 cut(s) 2422, 2539, 2820, 3119
BmeRI GACNNNNNGTC 1 cut(s) 464
BmeT110I CYCGRG 3 cut(s) 481, 2676, 2992
BmgBI CACGTC 4 cut(s) 488, 972, 1052, 1430
BmgT120I GGNCC 9 cut(s) 4, 1529, 2267, 2422, 2539, 2643, 2820, 3119, 3345
BmrFI CCNGG 8 cut(s) 415, 911, 1785, 1890, 2271, 2435, 2696, 3323
BmrI ACTGGG 2 cut(s) 2274, 2979
BmsI GCATC 7 cut(s) 661, 1117, 1168, 2188, 2573, 2659, 2964
BmuI ACTGGG 2 cut(s) 2274, 2979
BpiI GAAGAC 2 cut(s) 465, 2849
BplI GAGNNNNNCTC 8 cut(s) 379, 411, 730, 762, 2986, 3018, 3214, 3246
BpmI CTGGAG 2 cut(s) 1872, 2536
Bpu1102I GCTNAGC 1 cut(s) 1191
Bpu14I TTCGAA 2 cut(s) 383, 1040
BpuEI CTTGAG 2 cut(s) 2363, 3216
BpuMI CCSGG 1 cut(s) 2696
BsaAI YACGTR 1 cut(s) 2365
BsaJI CCNNGG 5 cut(s) 414, 2183, 2356, 2433, 3322
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 4 cut(s) 886, 916, 3086, 3116
Bsc4I CCNNNNNNNGG 7 cut(s) 1638, 1784, 2188, 2189, 2467, 2514, 2652
Bse21I CCTNAGG 1 cut(s) 2509
BseBI CCWGG 7 cut(s) 415, 911, 1785, 1890, 2271, 2435, 3323
BseDI CCNNGG 5 cut(s) 414, 2183, 2356, 2433, 3322
BseLI CCNNNNNNNGG 7 cut(s) 1638, 1784, 2188, 2189, 2467, 2514, 2652
BseRI GAGGAG 6 cut(s) 156, 1937, 1966, 1969, 2675, 3218
BseSI GKGCMC 1 cut(s) 1768
BseYI CCCAGC 4 cut(s) 1419, 1689, 2246, 2823
BsgI GTGCAG 3 cut(s) 860, 2788, 3004
Bsh1236I CGCG 1 cut(s) 30
BsiHKAI GWGCWC 4 cut(s) 397, 1768, 2482, 3004
BsiHKCI CYCGRG 3 cut(s) 481, 2676, 2992
BsiSI CCGG 4 cut(s) 194, 437, 2696, 2965
BslFI GGGAC 6 cut(s) 181, 498, 2678, 2774, 2806, 3132
BslI CCNNNNNNNGG 7 cut(s) 1638, 1784, 2188, 2189, 2467, 2514, 2652
BsmAI GTCTC 7 cut(s) 703, 768, 1469, 2105, 2388, 3169, 3307
BsmBI CGTCTC 1 cut(s) 3169
BsmFI GGGAC 6 cut(s) 181, 498, 2678, 2774, 2806, 3132
BsoBI CYCGRG 3 cut(s) 481, 2676, 2992
Bsp119I TTCGAA 2 cut(s) 383, 1040
Bsp1286I GDGCHC 6 cut(s) 397, 482, 1768, 2080, 2482, 3004
Bsp1407I TGTACA 2 cut(s) 1801, 2289
Bsp143I GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
Bsp1720I GCTNAGC 1 cut(s) 1191
BspACI CCGC 7 cut(s) 138, 191, 629, 776, 941, 3098, 3311
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 4 cut(s) 22, 889, 1908, 3043
BspMAI CTGCAG 2 cut(s) 1299, 1852
BspMI ACCTGC 1 cut(s) 1064
BspPI GGATC 5 cut(s) 804, 959, 972, 1676, 2335
BspQI GCTCTTC 2 cut(s) 2208, 2568
BspT104I TTCGAA 2 cut(s) 383, 1040
BsrBI CCGCTC 1 cut(s) 138
BsrGI TGTACA 2 cut(s) 1801, 2289
BssECI CCNNGG 5 cut(s) 414, 2183, 2356, 2433, 3322
BssMI GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
BssT1I CCWWGG 2 cut(s) 2183, 2356
Bst2UI CCWGG 7 cut(s) 415, 911, 1785, 1890, 2271, 2435, 3323
Bst4CI ACNGT 9 cut(s) 596, 773, 1157, 1396, 1403, 1540, 1610, 2125, 2736
Bst6I CTCTTC 9 cut(s) 268, 359, 380, 522, 701, 1461, 2208, 2568, 3167
BstAPI GCANNNNNTGC 2 cut(s) 1136, 1187
BstAUI TGTACA 2 cut(s) 1801, 2289
BstBAI YACGTR 1 cut(s) 2365
BstBI TTCGAA 2 cut(s) 383, 1040
BstC8I GCNNGC 8 cut(s) 566, 939, 1071, 2034, 2046, 2253, 2322, 3257
BstFNI CGCG 1 cut(s) 30
BstKTI GATC 8 cut(s) 28, 121, 799, 815, 967, 1671, 2343, 2707
BstMAI GTCTC 7 cut(s) 703, 768, 1469, 2105, 2388, 3169, 3307
BstMBI GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
BstNI CCWGG 7 cut(s) 415, 911, 1785, 1890, 2271, 2435, 3323
BstNSI RCATGY 5 cut(s) 161, 568, 2280, 2789, 3076
BstSCI CCNGG 8 cut(s) 413, 909, 1783, 1888, 2269, 2433, 2694, 3321
BstSFI CTRYAG 3 cut(s) 1140, 1295, 1848
BstSLI GKGCMC 1 cut(s) 1768
BstUI CGCG 1 cut(s) 30
BstV2I GAAGAC 2 cut(s) 465, 2849
BstX2I RGATCY 3 cut(s) 964, 1668, 2340
BstXI CCANNNNNNTGG 1 cut(s) 2246
BstYI RGATCY 3 cut(s) 964, 1668, 2340
Bsu36I CCTNAGG 1 cut(s) 2509
BsuI GTATCC 3 cut(s) 1813, 2620, 3142
BtgZI GCGATG 1 cut(s) 2682
BtrI CACGTC 4 cut(s) 488, 972, 1052, 1430
BtsI GCAGTG 5 cut(s) 1857, 2776, 3083, 3096, 3107
BveI ACCTGC 1 cut(s) 1064
Cac8I GCNNGC 8 cut(s) 566, 939, 1071, 2034, 2046, 2253, 2322, 3257
CaiI CAGNNNCTG 4 cut(s) 808, 1136, 1264, 3055
CciI TCATGA 4 cut(s) 22, 889, 1908, 3043
Cfr13I GGNCC 9 cut(s) 4, 1529, 2267, 2422, 2539, 2643, 2820, 3119, 3345
CseI GACGC 1 cut(s) 1682
CspCI CAANNNNNGTGG 2 cut(s) 1413, 1448
DpnI GATC 8 cut(s) 27, 120, 798, 814, 966, 1670, 2342, 2706
DpnII GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
DriI GACNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 2 cut(s) 981, 1915
Eam1104I CTCTTC 9 cut(s) 268, 359, 380, 522, 701, 1461, 2208, 2568, 3167
Eam1105I GACNNNNNGTC 1 cut(s) 464
EarI CTCTTC 9 cut(s) 268, 359, 380, 522, 701, 1461, 2208, 2568, 3167
Ecl136II GAGCTC 2 cut(s) 395, 2480
Eco130I CCWWGG 2 cut(s) 2183, 2356
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 4 cut(s) 397, 482, 2080, 2482
Eco32I GATATC 1 cut(s) 1897
Eco47I GGWCC 4 cut(s) 2422, 2539, 2820, 3119
Eco53kI GAGCTC 2 cut(s) 395, 2480
Eco57I CTGAAG 4 cut(s) 48, 92, 2408, 2861
Eco81I CCTNAGG 1 cut(s) 2509
Eco88I CYCGRG 3 cut(s) 481, 2676, 2992
EcoICRI GAGCTC 2 cut(s) 395, 2480
EcoO109I RGGNCCY 2 cut(s) 2820, 3119
EcoRI GAATTC 1 cut(s) 1223
EcoRII CCWGG 7 cut(s) 413, 909, 1783, 1888, 2269, 2433, 3321
EcoRV GATATC 1 cut(s) 1897
EcoT14I CCWWGG 2 cut(s) 2183, 2356
EcoT22I ATGCAT 1 cut(s) 2090
EcoT38I GRGCYC 4 cut(s) 397, 482, 2080, 2482
ErhI CCWWGG 2 cut(s) 2183, 2356
Esp3I CGTCTC 1 cut(s) 3169
FalI AAGNNNNNCTT 6 cut(s) 864, 896, 889, 921, 1799, 1831
FaqI GGGAC 6 cut(s) 181, 498, 2678, 2774, 2806, 3132
FauI CCCGC 1 cut(s) 3304
FauNDI CATATG 1 cut(s) 1197
FriOI GRGCYC 4 cut(s) 397, 482, 2080, 2482
FspBI CTAG 7 cut(s) 521, 1010, 2184, 2217, 2579, 2808, 2831
GsaI CCCAGC 4 cut(s) 1423, 1693, 2250, 2827
GsuI CTGGAG 2 cut(s) 1872, 2536
HapII CCGG 4 cut(s) 194, 437, 2696, 2965
HgaI GACGC 1 cut(s) 1682
HincII GTYRAC 1 cut(s) 1055
HindII GTYRAC 1 cut(s) 1055
HinfI GANTC 9 cut(s) 19, 98, 536, 640, 1589, 2699, 2836, 3052, 3143
HpaII CCGG 4 cut(s) 194, 437, 2696, 2965
HphI GGTGA 9 cut(s) 278, 325, 545, 1100, 1169, 1499, 1904, 2582, 2699
Hpy166II GTNNAC 6 cut(s) 1055, 1151, 1399, 1766, 2176, 2292
Hpy8I GTNNAC 6 cut(s) 1055, 1151, 1399, 1766, 2176, 2292
Hpy99I CGWCG 2 cut(s) 1688, 1698
HpyAV CCTTC 4 cut(s) 371, 611, 682, 2383
HpyCH4III ACNGT 9 cut(s) 596, 773, 1157, 1396, 1403, 1540, 1610, 2125, 2736
HpyCH4IV ACGT 9 cut(s) 487, 971, 1051, 1057, 1429, 2242, 2364, 2846, 2886
HpySE526I ACGT 9 cut(s) 487, 971, 1051, 1057, 1429, 2242, 2364, 2846, 2886
KflI GGGWCCC 1 cut(s) 3119
Kzo9I GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
LguI GCTCTTC 2 cut(s) 2208, 2568
LmnI GCTCC 5 cut(s) 392, 943, 1066, 2477, 3094
LweI GCATC 7 cut(s) 661, 1117, 1168, 2188, 2573, 2659, 2964
MaeI CTAG 7 cut(s) 521, 1010, 2184, 2217, 2579, 2808, 2831
MaeII ACGT 9 cut(s) 487, 971, 1051, 1057, 1429, 2242, 2364, 2846, 2886
MalI GATC 8 cut(s) 27, 120, 798, 814, 966, 1670, 2342, 2706
MbiI CCGCTC 1 cut(s) 138
MboI GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
MfeI CAATTG 1 cut(s) 2604
MflI RGATCY 3 cut(s) 964, 1668, 2340
MhlI GDGCHC 6 cut(s) 397, 482, 1768, 2080, 2482, 3004
MlsI TGGCCA 2 cut(s) 983, 1917
MluNI TGGCCA 2 cut(s) 983, 1917
MlyI GAGTC 4 cut(s) 28, 649, 2708, 3152
MmeI TCCRAC 2 cut(s) 1312, 3126
Mox20I TGGCCA 2 cut(s) 983, 1917
Mph1103I ATGCAT 1 cut(s) 2090
MroXI GAANNNNTTC 2 cut(s) 2442, 3051
MscI TGGCCA 2 cut(s) 983, 1917
MseI TTAA 5 cut(s) 1704, 1731, 2790, 2937, 2958
MslI CAYNNNNRTG 4 cut(s) 162, 1125, 1383, 1907
Msp20I TGGCCA 2 cut(s) 983, 1917
MspA1I CMGCKG 5 cut(s) 629, 2029, 2036, 2464, 2827
MspI CCGG 4 cut(s) 194, 437, 2696, 2965
MspR9I CCNGG 8 cut(s) 415, 911, 1785, 1890, 2271, 2435, 2696, 3323
MunI CAATTG 1 cut(s) 2604
MvaI CCWGG 7 cut(s) 415, 911, 1785, 1890, 2271, 2435, 3323
MvnI CGCG 1 cut(s) 30
NciI CCSGG 1 cut(s) 2696
NdeI CATATG 1 cut(s) 1197
NdeII GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
NmuCI GTSAC 6 cut(s) 590, 1522, 1641, 1714, 1852, 2771
NsiI ATGCAT 1 cut(s) 2090
NspI RCATGY 5 cut(s) 161, 568, 2280, 2789, 3076
NspV TTCGAA 2 cut(s) 383, 1040
PaeI GCATGC 1 cut(s) 568
PagI TCATGA 4 cut(s) 22, 889, 1908, 3043
PceI AGGCCT 1 cut(s) 901
PciI ACATGT 2 cut(s) 157, 2276
PciSI GCTCTTC 2 cut(s) 2208, 2568
PcsI WCGNNNNNNNCGW 3 cut(s) 34, 701, 2248
PdmI GAANNNNTTC 2 cut(s) 2442, 3051
PfeI GAWTC 5 cut(s) 98, 536, 1589, 2836, 3052
PfoI TCCNGGA 3 cut(s) 909, 1783, 2694
PleI GAGTC 4 cut(s) 27, 648, 2707, 3151
PpsI GAGTC 4 cut(s) 27, 648, 2707, 3151
Ppu21I YACGTR 1 cut(s) 2365
PpuMI RGGWCCY 2 cut(s) 2820, 3119
PscI ACATGT 2 cut(s) 157, 2276
Psp124BI GAGCTC 2 cut(s) 397, 2482
Psp1406I AACGTT 1 cut(s) 1057
Psp5II RGGWCCY 2 cut(s) 2820, 3119
Psp6I CCWGG 7 cut(s) 413, 909, 1783, 1888, 2269, 2433, 3321
PspFI CCCAGC 4 cut(s) 1419, 1689, 2246, 2823
PspGI CCWGG 7 cut(s) 413, 909, 1783, 1888, 2269, 2433, 3321
PspPI GGNCC 9 cut(s) 4, 1529, 2267, 2422, 2539, 2643, 2820, 3119, 3345
PspPPI RGGWCCY 2 cut(s) 2820, 3119
PsrI GAACNNNNNNTAC 2 cut(s) 2345, 2377
PstI CTGCAG 2 cut(s) 1299, 1852
PstNI CAGNNNCTG 4 cut(s) 808, 1136, 1264, 3055
PsuI RGATCY 3 cut(s) 964, 1668, 2340
PvuII CAGCTG 4 cut(s) 2029, 2036, 2464, 2827
RseI CAYNNNNRTG 4 cut(s) 162, 1125, 1383, 1907
SacI GAGCTC 2 cut(s) 397, 2482
SapI GCTCTTC 2 cut(s) 2208, 2568
SaqAI TTAA 5 cut(s) 1704, 1731, 2790, 2937, 2958
Sau3AI GATC 8 cut(s) 25, 118, 796, 812, 964, 1668, 2340, 2704
Sau96I GGNCC 9 cut(s) 4, 1529, 2267, 2422, 2539, 2643, 2820, 3119, 3345
ScaI AGTACT 2 cut(s) 1566, 1814
SchI GAGTC 4 cut(s) 28, 649, 2708, 3152
ScrFI CCNGG 8 cut(s) 415, 911, 1785, 1890, 2271, 2435, 2696, 3323
SduI GDGCHC 6 cut(s) 397, 482, 1768, 2080, 2482, 3004
SfaNI GCATC 7 cut(s) 661, 1117, 1168, 2188, 2573, 2659, 2964
SfcI CTRYAG 3 cut(s) 1140, 1295, 1848
SfuI TTCGAA 2 cut(s) 383, 1040
SinI GGWCC 4 cut(s) 2422, 2539, 2820, 3119
SmiMI CAYNNNNRTG 4 cut(s) 162, 1125, 1383, 1907
SmlI CTYRAG 2 cut(s) 2378, 3231
SmoI CTYRAG 2 cut(s) 2378, 3231
SphI GCATGC 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 7 cut(s) 138, 191, 629, 776, 941, 3098, 3311
SspI AATATT 2 cut(s) 2104, 2443
SspMI CTAG 7 cut(s) 521, 1010, 2184, 2217, 2579, 2808, 2831
SstI GAGCTC 2 cut(s) 397, 2482
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 8 cut(s) 413, 909, 1783, 1888, 2269, 2433, 2694, 3321
StyI CCWWGG 2 cut(s) 2183, 2356
TaaI ACNGT 9 cut(s) 596, 773, 1157, 1396, 1403, 1540, 1610, 2125, 2736
TaiI ACGT 9 cut(s) 490, 974, 1054, 1060, 1432, 2245, 2367, 2849, 2889
TaqI TCGA 5 cut(s) 383, 682, 1040, 1306, 2484
TatI WGTACW 6 cut(s) 1548, 1564, 1801, 1812, 2289, 3069
TauI GCSGC 2 cut(s) 193, 3100
TfiI GAWTC 5 cut(s) 98, 536, 1589, 2836, 3052
Tru1I TTAA 5 cut(s) 1704, 1731, 2790, 2937, 2958
Tru9I TTAA 5 cut(s) 1704, 1731, 2790, 2937, 2958
TseFI GTSAC 6 cut(s) 590, 1522, 1641, 1714, 1852, 2771
Tsp45I GTSAC 6 cut(s) 590, 1522, 1641, 1714, 1852, 2771
TspGWI ACGGA 2 cut(s) 185, 710
VneI GTGCAC 1 cut(s) 1764
VpaK11BI GGWCC 4 cut(s) 2422, 2539, 2820, 3119
XapI RAATTY 7 cut(s) 1223, 1302, 1742, 1869, 2595, 2684, 2853
XceI RCATGY 5 cut(s) 161, 568, 2280, 2789, 3076
XcmI CCANNNNNNNNNTGG 1 cut(s) 976
XmaJI CCTAGG 1 cut(s) 2183
XmnI GAANNNNTTC 2 cut(s) 2442, 3051
XspI CTAG 7 cut(s) 521, 1010, 2184, 2217, 2579, 2808, 2831
ZrmI AGTACT 2 cut(s) 1566, 1814
Zsp2I ATGCAT 1 cut(s) 2090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.