Rh4BG315000

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002001
Physical Location & Seq
Reverse (-)
48219204 .. 48221504
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG315000.1

Sequence Viewer

Length: 888 bp
ATGCCAGTAGCAAGAGACATTGAATCATTGATAATCCTTTTATTGTTACTTGATATTTTCAGAACATTTGTTAATTTACTCTACAAATTAATTTTAAGCTCCCGTGAAGGTTGGAACAACAAGGAAGATGGTAGCAGTACGGAATATGGAAAGAGGTGCGGAGAAGTCAATCTTTCTATCTTAGAAAATTTGTTCCATCTGCGCCATAAGTTTGCTCGGTTACTTGGCTATTCAAACTATGCTAACTATGCTGTTGATCTTAGAATGGCAAAGACGCCCACAAAGTGGAAACTACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCCCTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTCCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTCGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTAGGGCCCATATTCTTCTCTGTGAAGGATTCAGATACATCGCTATTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACATTTATGGGGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAGGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAGGCCAAAGAAACTTAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

295

Amino Acids

34.22

Weight (kDa)

9.23

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 147 - 224 9.2e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 285
AciI CCGC 2 cut(s) 159, 659
AclWI GGATC 2 cut(s) 363, 822
AcsI RAATTY 2 cut(s) 187, 846
AcyI GRCGYC 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 2 cut(s) 139, 620
AfiI CCNNNNNNNGG 2 cut(s) 285, 519
AgsI TTSAA 5 cut(s) 23, 234, 391, 487, 852
AjnI CCWGG 1 cut(s) 856
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 4 cut(s) 99, 376, 449, 882
AluI AGCT 4 cut(s) 99, 376, 449, 882
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 2 cut(s) 9, 718
AlwI GGATC 2 cut(s) 363, 822
AoxI GGCC 3 cut(s) 422, 668, 866
ApaI GGGCCC 1 cut(s) 672
ApeKI GCWGC 4 cut(s) 376, 835, 838, 841
ApoI RAATTY 2 cut(s) 187, 846
AseI ATTAAT 1 cut(s) 89
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 3 cut(s) 322, 668, 669
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 672
BaeI ACNNNNGTAYC 2 cut(s) 284, 317
BanII GRGCYC 1 cut(s) 672
BbsI GAAGAC 2 cut(s) 398, 746
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 4 cut(s) 363, 847, 850, 853
BccI CCATC 4 cut(s) 122, 204, 540, 754
BciT130I CCWGG 1 cut(s) 858
BcoDI GTCTC 2 cut(s) 9, 718
BfaI CTAG 3 cut(s) 357, 380, 480
BfmI CTRYAG 1 cut(s) 636
BglII AGATCT 1 cut(s) 384
BisI GCNGC 4 cut(s) 377, 836, 839, 842
BlsI GCNGC 4 cut(s) 378, 837, 840, 843
Bme1390I CCNGG 1 cut(s) 858
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 322, 668, 669
BmiI GGNNCC 3 cut(s) 323, 645, 670
BmrFI CCNGG 1 cut(s) 858
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 2 cut(s) 513, 557
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 398, 746
BplI GAGNNNNNCTC 4 cut(s) 535, 567, 763, 795
BpmI CTGGAG 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 519
Bse1I ACTGG 3 cut(s) 5, 317, 523
BseBI CCWGG 1 cut(s) 858
BseGI GGATG 3 cut(s) 528, 538, 572
BseLI CCNNNNNNNGG 2 cut(s) 285, 519
BseMII CTCAG 1 cut(s) 761
BseNI ACTGG 3 cut(s) 5, 317, 523
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 4 cut(s) 363, 847, 850, 853
BseYI CCCAGC 1 cut(s) 372
BshFI GGCC 3 cut(s) 424, 670, 868
BsiHKAI GWGCWC 1 cut(s) 553
BslFI GGGAC 2 cut(s) 323, 335
BslI CCNNNNNNNGG 2 cut(s) 285, 519
BsmAI GTCTC 2 cut(s) 9, 718
BsmFI GGGAC 2 cut(s) 323, 335
BsnI GGCC 3 cut(s) 424, 670, 868
Bsp120I GGGCCC 1 cut(s) 668
Bsp1286I GDGCHC 2 cut(s) 553, 672
Bsp143I GATC 4 cut(s) 256, 368, 384, 827
BspACI CCGC 2 cut(s) 159, 659
BspANI GGCC 3 cut(s) 424, 670, 868
BspCNI CTCAG 1 cut(s) 762
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 3 cut(s) 323, 645, 670
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 2 cut(s) 363, 822
BsrI ACTGG 3 cut(s) 5, 317, 523
BssMI GATC 4 cut(s) 256, 368, 384, 827
BssNI GRCGYC 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 858
Bst4CI ACNGT 1 cut(s) 651
Bst6I CTCTTC 2 cut(s) 716, 729
BstACI GRCGYC 1 cut(s) 275
BstDEI CTNAG 4 cut(s) 181, 260, 770, 878
BstF5I GGATG 3 cut(s) 528, 538, 572
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 4 cut(s) 259, 371, 387, 830
BstMAI GTCTC 2 cut(s) 9, 718
BstMBI GATC 4 cut(s) 256, 368, 384, 827
BstMWI GCNNNNNNNGC 3 cut(s) 248, 712, 841
BstNI CCWGG 1 cut(s) 858
BstNSI RCATGY 2 cut(s) 338, 625
BstSCI CCNGG 1 cut(s) 856
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 672
BstV1I GCAGC 4 cut(s) 363, 847, 850, 853
BstV2I GAAGAC 2 cut(s) 398, 746
BstX2I RGATCY 3 cut(s) 368, 384, 827
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 3 cut(s) 368, 384, 827
BsuRI GGCC 3 cut(s) 424, 670, 868
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 3 cut(s) 528, 538, 572
BtsI GCAGTG 4 cut(s) 553, 632, 633, 645
BtsIMutI CAGTG 7 cut(s) 395, 429, 553, 632, 633, 645, 656
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 322, 668, 669
CseI GACGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 138, 619
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviAII CATG 4 cut(s) 335, 528, 593, 622
CviQI GTAC 2 cut(s) 138, 619
DdeI CTNAG 4 cut(s) 181, 260, 770, 878
DpnI GATC 4 cut(s) 258, 370, 386, 829
DpnII GATC 4 cut(s) 256, 368, 384, 827
DraIII CACNNNGTG 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 716, 729
EarI CTCTTC 2 cut(s) 716, 729
EciI GGCGGA 1 cut(s) 674
Eco24I GRGCYC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 856
EcoT38I GRGCYC 1 cut(s) 672
FaeI CATG 4 cut(s) 338, 531, 596, 625
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 2 cut(s) 323, 335
FatI CATG 4 cut(s) 334, 527, 592, 621
Fnu4HI GCNGC 4 cut(s) 377, 836, 839, 842
FokI GGATG 3 cut(s) 525, 535, 579
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 4 cut(s) 377, 836, 839, 842
FspBI CTAG 3 cut(s) 357, 380, 480
GlaI GCGC 1 cut(s) 203
GluI GCNGC 4 cut(s) 377, 836, 839, 842
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 3 cut(s) 424, 670, 868
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 4 cut(s) 338, 531, 596, 625
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HinfI GANTC 3 cut(s) 23, 692, 766
HphI GGTGA 1 cut(s) 376
Hpy188I TCNGA 4 cut(s) 62, 543, 697, 771
Hpy188III TCNNGA 4 cut(s) 388, 513, 575, 593
HpyAV CCTTC 4 cut(s) 101, 481, 607, 682
HpyCH4III ACNGT 1 cut(s) 651
HpyCH4V TGCA 4 cut(s) 334, 570, 625, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 712, 841
HpyF3I CTNAG 4 cut(s) 181, 260, 770, 878
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 4 cut(s) 338, 531, 596, 625
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 4 cut(s) 256, 368, 384, 827
LmnI GCTCC 3 cut(s) 104, 643, 712
Lsp1109I GCAGC 4 cut(s) 363, 847, 850, 853
LweI GCATC 2 cut(s) 513, 557
MaeI CTAG 3 cut(s) 357, 380, 480
MaeIII GTNAC 3 cut(s) 45, 219, 345
MalI GATC 4 cut(s) 258, 370, 386, 829
MboI GATC 4 cut(s) 256, 368, 384, 827
MboII GAAGA 6 cut(s) 137, 403, 670, 733, 746, 751
MflI RGATCY 3 cut(s) 368, 384, 827
MhlI GDGCHC 2 cut(s) 553, 672
MluCI AATT 7 cut(s) 73, 86, 90, 187, 472, 508, 846
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 6 cut(s) 147, 299, 355, 781, 804, 858
MseI TTAA 5 cut(s) 72, 89, 95, 339, 507
MslI CAYNNNNRTG 1 cut(s) 638
MspA1I CMGCKG 1 cut(s) 376
MspR9I CCNGG 1 cut(s) 858
MvaI CCWGG 1 cut(s) 858
MwoI GCNNNNNNNGC 3 cut(s) 248, 712, 841
NdeII GATC 4 cut(s) 256, 368, 384, 827
NlaIII CATG 4 cut(s) 338, 531, 596, 625
NlaIV GGNNCC 3 cut(s) 323, 645, 670
NspI RCATGY 2 cut(s) 338, 625
OliI CACNNNNGTG 1 cut(s) 638
PagI TCATGA 1 cut(s) 592
PfeI GAWTC 3 cut(s) 23, 692, 766
PflFI GACNNNGTC 1 cut(s) 581
PflMI CCANNNNNTGG 1 cut(s) 285
PkrI GCNGC 4 cut(s) 378, 837, 840, 843
PshBI ATTAAT 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 856
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 1 cut(s) 856
PspN4I GGNNCC 3 cut(s) 323, 645, 670
PspOMI GGGCCC 1 cut(s) 668
PspPI GGNCC 3 cut(s) 322, 668, 669
PstI CTGCAG 1 cut(s) 640
PsuI RGATCY 3 cut(s) 368, 384, 827
PsyI GACNNNGTC 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 139, 620
RsaNI GTAC 2 cut(s) 138, 619
RseI CAYNNNNRTG 1 cut(s) 638
SaqAI TTAA 5 cut(s) 72, 89, 95, 339, 507
SatI GCNGC 4 cut(s) 377, 836, 839, 842
Sau3AI GATC 4 cut(s) 256, 368, 384, 827
Sau96I GGNCC 3 cut(s) 322, 668, 669
ScrFI CCNGG 1 cut(s) 858
SduI GDGCHC 2 cut(s) 553, 672
SetI ASST 7 cut(s) 101, 112, 158, 366, 378, 451, 884
SfaNI GCATC 2 cut(s) 513, 557
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
Sse9I AATT 7 cut(s) 73, 86, 90, 187, 472, 508, 846
SsiI CCGC 2 cut(s) 159, 659
SspMI CTAG 3 cut(s) 357, 380, 480
StyD4I CCNGG 1 cut(s) 856
TaaI ACNGT 1 cut(s) 651
TaqI TCGA 1 cut(s) 304
TasI AATT 7 cut(s) 73, 86, 90, 187, 472, 508, 846
TatI WGTACW 1 cut(s) 618
TfiI GAWTC 3 cut(s) 23, 692, 766
Tru1I TTAA 5 cut(s) 72, 89, 95, 339, 507
Tru9I TTAA 5 cut(s) 72, 89, 95, 339, 507
TscAI CASTG 7 cut(s) 402, 436, 553, 632, 640, 645, 656
TseI GCWGC 4 cut(s) 376, 835, 838, 841
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 7 cut(s) 402, 436, 553, 632, 640, 645, 656
Tth111I GACNNNGTC 1 cut(s) 581
Van91I CCANNNNNTGG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 322
VspI ATTAAT 1 cut(s) 89
XapI RAATTY 2 cut(s) 187, 846
XceI RCATGY 2 cut(s) 338, 625
XspI CTAG 3 cut(s) 357, 380, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.