pycom10g18790

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002001
Physical Location & Seq
Reverse (-)
22049134 .. 22051560
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g18790.1

Sequence Viewer

Length: 2427 bp
ATGCCAATAAAGGATTCCACGTCTGATATGGCCATAATTTCTCGCAAAGGGTCTGCTCTCATTCGGAAAATTCATGAGAAGCAAAATATGAACAAATCGCGTCAACGTTTTTGGGAGCTTGCAGGTTCTAAACTAGGCGATATACTTGGTGTTGAGAAAACAGCGGAGCAGATTGATGCAGATACTGCTGCTGCAGGTGCAGACGGTGAAATTGATTTCAAAGAAGATGCCAAGTTTGCACAGCATATGAAGAATGAGGAAGCAGCGAGTGAATTTGCAAAGTCAAAAACCATCTCACAGCAACGACAGTTTTTGCCCATATATTCTGTGAGGGATGATTTACTACAGGTAATTCGAGAAAATCAAGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACGACTCAACTGACACAGTATCTGCACGAAGAGGGTTACACTGTAAACGGCATTGTGGGTTGCACCCAACCAAGACGTGTGGCAGCCATGAGTGTTGCAAAGAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGATAAAGTTGGGTACGCTATTCGGTTTGAGGATGTGACTGGACCAAATACCATAATAAAATACATGACTGATGGAGTACTTATGCATGAAACACTAAGAGATTCTGATCTAGACAAGTATCGCGTAGTTGTGATGGACGAAGCTCATGAAAGGTCACTGAACACGGATGTGCTGCTTGGGATTTTGAAAACAGTGGTTGCCCGGCGGCGTGATTTTAAGCTCATCGTGACATCTGCAACACTAGATGCTCAGAAGTTTAGAAACTTTTTTGGAAGTGCACCGATTTTTGAAATTCCTGGGAGAACATTTCCAGTAAGTATCAGGTATAGTAAAACCCCATGTGAAGATTATGTTGAAGCTGCAGTGAAGGAGGCCATAACTATTCATATCACCGGCACTGAAGGTGACATCCTCATCTTCATGACCGGCCAGGATGAGATTGAGGCAGCTTGTTATGCCCTTTCAGAGCGGATGGAACAAATTATCTCCACCTCGGTTAAAGAGGTGCCAAAACTTTTGATACTCCCTATATATTCACAGTTGCCTGCTGACTTGCAAGCAAAGATATTCCAAAAAGCTGAAGATGGGGCTCGTAAATGTATTGTGGCAACCAACATTGCTGAGACATCTTTGACGGTAGATGGAATCTCATATGTCATCGACACGGGATATGGTAAAATGAAAGTATACAACCCGAAAATGGGTATGGATGCTCTTCAAGTATTTCCAATTAGTCAGACTGCTGCTAACCAACGTTCTGGGCGTGCTGGCAGAACTAAGCCTGGAACATGTTATCGGCTGTACACAGAGAATGCGTACTTAAATGAAATGCTGCCCAATCCTGTGCCAGAGATCCAGAGGACCAACCTCGGATATGTGATCTTGTTGCTCAAATCACTTCAGGTTGATAAGATACTAGATTTTGATTTCGTAGACCCACCTCCCAAGGATAATATTCTCCATTCTTTGTACCAGTTGTGGGTGTTGGGTGCTCTTAACAATGAGGGGCGGTTAACTGACCTGGGCTGGAAAATGGTGGAATTGCCATTGGACCCTCCACTCGCTAAAATGCTATTGATGGGTGAAAAGCTAGGATGTTTAGATGAGGTTTTGACAATTGTGTCCATGCTTTCAGTGCCTTCGGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGTGATGCTGCAAGGGAGAAATTCTCCTTCCCAGAGTCCGACCACTTGACGTTGTACAATGTTTATCAGCAGTGGGAACAACATCAGTATCGTGGGGACTGGTGTGATGACCACTTTTTGCAAGTTAAAGGGCTGCGAAAGGTTAGAGAGGTGAGGTCCCAACTGCTAGAGATACTAAAGACACTCAAAGTCAAAGTCCCACTCACATCCTGTTGGCCTGATACTGATAAGGTGAGACAAGCTATTTGCTCTGCGTACTTTCAGAATGCAGCAAGACTAAAGGGTGTGGGGGAATATGTTAACTGCCGGACTGGAATGCCATGCTATTTACACCCGAGCAGTGCGCTTTATGGCATGGGCTGCAATCCAGAGTATGTGGTCTATCATGAGCTAGTTTTGACAAGGAAGGAGTATATGCAGTGTGCCACTGCAGTGGAACCGCAATGGTTGGCTGAGTTCGGATCCATGTTCTATTATGTGAAGGAGTCGGATAACTCAATGTTGGAGCACAAGAAGAGACAAAAGGGGGAAAAGACAGCGATGCAGGCGGAAATGGAGAATCTGAGAAAGGCTCGAGCAGAGGCAGAGAAAGCAAGCAAGCAGAAGGAGAGGGAAAAGAGATCCAAGCAGCAGCAGCAGCAGCAAGTCGTCTCAATGCCGGGTTCGCACCAGGGCTCTTCTACATACTTGAGGCCGAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

809

Amino Acids

91.28

Weight (kDa)

6.74

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 115 - 264 6.6e-09 DEAD/DEAH box helicase
AAA_22 PF13401 120 - 254 3.5e-06 AAA domain
Helicase_C PF00271 310 - 438 1.2e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 502 - 529 3.1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 530 - 589 1e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 649 - 726 3.8e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 185
AasI GACNNNNNNGTC 1 cut(s) 1657
Acc36I ACCTGC 2 cut(s) 113, 185
AccB1I GGYRCC 1 cut(s) 1042
AccBSI CCGCTC 1 cut(s) 1006
AccI GTMKAC 2 cut(s) 1224, 1470
AccII CGCG 2 cut(s) 100, 660
AciI CCGC 6 cut(s) 164, 742, 1006, 1546, 2153, 2261
AclI AACGTT 2 cut(s) 106, 1291
AclWI GGATC 4 cut(s) 1384, 2169, 2182, 2328
AcoI YGGCCR 2 cut(s) 30, 964
AcsI RAATTY 4 cut(s) 69, 272, 828, 1733
AcuI CTGAAG 3 cut(s) 957, 1137, 1421
AfaI GTAC 7 cut(s) 551, 615, 1340, 1355, 1508, 1769, 1970
AfiI CCNNNNNNNGG 4 cut(s) 1237, 1238, 1516, 1701
AflIII ACRYGT 2 cut(s) 478, 1325
AgsI TTSAA 5 cut(s) 220, 724, 827, 893, 1256
AjiI CACGTC 2 cut(s) 21, 479
AjnI CCWGG 5 cut(s) 832, 966, 1318, 1557, 2382
AjuI GAANNNNNNNTTGG 1 cut(s) 30
AleI CACNNNNGTG 2 cut(s) 704, 2144
Alw21I GWGCWC 3 cut(s) 817, 1531, 2223
Alw26I GTCTC 5 cut(s) 518, 1154, 1942, 2224, 2368
Alw44I GTGCAC 1 cut(s) 813
AlwI GGATC 4 cut(s) 1384, 2169, 2182, 2328
AlwNI CAGNNNCTG 2 cut(s) 185, 424
Ama87I CYCGRG 2 cut(s) 2047, 2286
AoxI GGCC 6 cut(s) 30, 909, 964, 1691, 1928, 2405
ApaLI GTGCAC 1 cut(s) 813
ApoI RAATTY 4 cut(s) 69, 272, 828, 1733
ArsI GACNNNNNNTTYG 4 cut(s) 1893, 1925, 1941, 1973
AspLEI GCGC 1 cut(s) 2059
AspS9I GGNCC 5 cut(s) 578, 1398, 1588, 1692, 1869
AsuC2I CCSGG 2 cut(s) 739, 2373
AsuHPI GGTGA 7 cut(s) 218, 548, 919, 953, 1631, 1876, 1957
AvaI CYCGRG 2 cut(s) 2047, 2286
AvaII GGWCC 4 cut(s) 578, 1398, 1588, 1869
BaeGI GKGCMC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 1784, 1817
BalI TGGCCA 1 cut(s) 32
BamHI GGATCC 1 cut(s) 2174
BanI GGYRCC 1 cut(s) 1042
BanII GRGCYC 2 cut(s) 1129, 2390
Bbv12I GWGCWC 3 cut(s) 817, 1531, 2223
BccI CCATC 8 cut(s) 299, 514, 602, 664, 1003, 1115, 1172, 1609
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 5 cut(s) 834, 968, 1320, 1559, 2384
BcnI CCSGG 2 cut(s) 739, 2373
BcoDI GTCTC 5 cut(s) 518, 1154, 1942, 2224, 2368
BfaI CTAG 7 cut(s) 134, 647, 779, 1454, 1628, 1880, 2105
BfmI CTRYAG 4 cut(s) 192, 344, 897, 2142
BfuAI ACCTGC 2 cut(s) 113, 185
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 7 cut(s) 739, 834, 968, 1320, 1559, 2373, 2384
Bme18I GGWCC 4 cut(s) 578, 1398, 1588, 1869
BmeT110I CYCGRG 2 cut(s) 2047, 2286
BmgBI CACGTC 2 cut(s) 21, 479
BmgT120I GGNCC 5 cut(s) 578, 1398, 1588, 1692, 1869
BmiI GGNNCC 5 cut(s) 1044, 1590, 1871, 2151, 2176
BmrFI CCNGG 7 cut(s) 739, 834, 968, 1320, 1559, 2373, 2384
BmsI GCATC 6 cut(s) 166, 217, 772, 1237, 1708, 2244
BplI GAGNNNNNCTC 4 cut(s) 1721, 1753, 2269, 2301
BpuEI CTTGAG 1 cut(s) 2422
BpuMI CCSGG 2 cut(s) 739, 2373
BsaBI GATNNNNATC 1 cut(s) 642
BsaJI CCNNGG 6 cut(s) 833, 1029, 1405, 1482, 1558, 2383
Bsc4I CCNNNNNNNGG 4 cut(s) 1237, 1238, 1516, 1701
Bse118I RCCGGY 2 cut(s) 929, 962
Bse1I ACTGG 6 cut(s) 394, 580, 848, 1510, 1817, 2029
Bse3DI GCAATG 2 cut(s) 1152, 2162
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 5 cut(s) 834, 968, 1320, 1559, 2384
BseDI CCNNGG 6 cut(s) 833, 1029, 1405, 1482, 1558, 2383
BseGI GGATG 9 cut(s) 340, 574, 709, 945, 976, 1014, 1252, 1637, 1919
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 4 cut(s) 1237, 1238, 1516, 1701
BseMI GCAATG 2 cut(s) 1152, 2162
BseMII CTCAG 5 cut(s) 519, 800, 1149, 2157, 2267
BseNI ACTGG 6 cut(s) 394, 580, 848, 1510, 1817, 2029
BseRI GAGGAG 1 cut(s) 1724
BseSI GKGCMC 1 cut(s) 817
BsgI GTGCAG 2 cut(s) 219, 410
Bsh1236I CGCG 2 cut(s) 100, 660
BshFI GGCC 6 cut(s) 32, 911, 966, 1693, 1930, 2407
BshNI GGYRCC 1 cut(s) 1042
BsiHKAI GWGCWC 3 cut(s) 817, 1531, 2223
BsiHKCI CYCGRG 2 cut(s) 2047, 2286
BsiSI CCGG 5 cut(s) 739, 930, 963, 2020, 2372
BslFI GGGAC 3 cut(s) 1823, 1855, 1895
BslI CCNNNNNNNGG 4 cut(s) 1237, 1238, 1516, 1701
BsmAI GTCTC 5 cut(s) 518, 1154, 1942, 2224, 2368
BsmBI CGTCTC 1 cut(s) 2368
BsmFI GGGAC 3 cut(s) 1823, 1855, 1895
BsmI GAATGC 3 cut(s) 1354, 1984, 2034
BsnI GGCC 6 cut(s) 32, 911, 966, 1693, 1930, 2407
BsoBI CYCGRG 2 cut(s) 2047, 2286
Bsp1286I GDGCHC 5 cut(s) 817, 1129, 1531, 2223, 2390
Bsp1407I TGTACA 2 cut(s) 1338, 1767
Bsp143I GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
BspACI CCGC 6 cut(s) 164, 742, 1006, 1546, 2153, 2261
BspANI GGCC 6 cut(s) 32, 911, 966, 1693, 1930, 2407
BspCNI CTCAG 5 cut(s) 520, 799, 1150, 2158, 2268
BspFNI CGCG 2 cut(s) 100, 660
BspHI TCATGA 4 cut(s) 73, 682, 957, 2098
BspLI GGNNCC 5 cut(s) 1044, 1590, 1871, 2151, 2176
BspMAI CTGCAG 3 cut(s) 196, 901, 2146
BspMI ACCTGC 2 cut(s) 113, 185
BspPI GGATC 4 cut(s) 1384, 2169, 2182, 2328
BspQI GCTCTTC 2 cut(s) 1257, 2395
BspT107I GGYRCC 1 cut(s) 1042
BsrBI CCGCTC 1 cut(s) 1006
BsrDI GCAATG 2 cut(s) 1152, 2162
BsrFI RCCGGY 2 cut(s) 929, 962
BsrGI TGTACA 2 cut(s) 1338, 1767
BsrI ACTGG 6 cut(s) 394, 580, 848, 1510, 1817, 2029
BssAI RCCGGY 2 cut(s) 929, 962
BssECI CCNNGG 6 cut(s) 833, 1029, 1405, 1482, 1558, 2383
BssMI GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
BssNAI GTATAC 1 cut(s) 1225
BssT1I CCWWGG 1 cut(s) 1482
Bst1107I GTATAC 1 cut(s) 1225
Bst2UI CCWGG 5 cut(s) 834, 968, 1320, 1559, 2384
Bst4CI ACNGT 7 cut(s) 206, 309, 420, 445, 730, 1077, 1174
Bst6I CTCTTC 5 cut(s) 426, 510, 1257, 2222, 2395
BstAPI GCANNNNNTGC 2 cut(s) 185, 2073
BstAUI TGTACA 2 cut(s) 1338, 1767
BstC8I GCNNGC 8 cut(s) 120, 1083, 1095, 1302, 1306, 2259, 2308, 2312
BstDEI CTNAG 7 cut(s) 528, 632, 786, 1158, 1314, 2166, 2276
BstF5I GGATG 9 cut(s) 340, 574, 709, 945, 976, 1014, 1252, 1637, 1919
BstFNI CGCG 2 cut(s) 100, 660
BstHHI GCGC 1 cut(s) 2059
BstKTI GATC 5 cut(s) 646, 1392, 1419, 2177, 2336
BstMAI GTCTC 5 cut(s) 518, 1154, 1942, 2224, 2368
BstMBI GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
BstNI CCWGG 5 cut(s) 834, 968, 1320, 1559, 2384
BstNSI RCATGY 1 cut(s) 1329
BstSCI CCNGG 7 cut(s) 737, 832, 966, 1318, 1557, 2371, 2382
BstSFI CTRYAG 4 cut(s) 192, 344, 897, 2142
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 2 cut(s) 100, 660
BstX2I RGATCY 3 cut(s) 1389, 2174, 2333
BstXI CCANNNNNNTGG 2 cut(s) 1295, 2146
BstYI RGATCY 3 cut(s) 1389, 2174, 2333
BstZ17I GTATAC 1 cut(s) 1225
BsuRI GGCC 6 cut(s) 32, 911, 966, 1693, 1930, 2407
BtgZI GCGATG 1 cut(s) 2267
BtrI CACGTC 2 cut(s) 21, 479
BtsCI GGATG 9 cut(s) 340, 574, 709, 945, 976, 1014, 1252, 1637, 1919
BtsI GCAGTG 6 cut(s) 906, 1790, 2059, 2138, 2139, 2151
BveI ACCTGC 2 cut(s) 113, 185
Cac8I GCNNGC 8 cut(s) 120, 1083, 1095, 1302, 1306, 2259, 2308, 2312
CaiI CAGNNNCTG 2 cut(s) 185, 424
CciI TCATGA 4 cut(s) 73, 682, 957, 2098
CfoI GCGC 1 cut(s) 2059
Cfr10I RCCGGY 2 cut(s) 929, 962
Cfr13I GGNCC 5 cut(s) 578, 1398, 1588, 1692, 1869
CseI GACGC 1 cut(s) 89
Csp6I GTAC 7 cut(s) 550, 614, 1339, 1354, 1507, 1768, 1969
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviQI GTAC 7 cut(s) 550, 614, 1339, 1354, 1507, 1768, 1969
DdeI CTNAG 7 cut(s) 528, 632, 786, 1158, 1314, 2166, 2276
DpnI GATC 5 cut(s) 645, 1391, 1418, 2176, 2335
DpnII GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
DrdI GACNNNNNNGTC 1 cut(s) 1657
DseDI GACNNNNNNGTC 1 cut(s) 1657
EaeI YGGCCR 2 cut(s) 30, 964
Eam1104I CTCTTC 5 cut(s) 426, 510, 1257, 2222, 2395
EarI CTCTTC 5 cut(s) 426, 510, 1257, 2222, 2395
EciI GGCGGA 1 cut(s) 2276
Eco130I CCWWGG 1 cut(s) 1482
Eco24I GRGCYC 2 cut(s) 1129, 2390
Eco47I GGWCC 4 cut(s) 578, 1398, 1588, 1869
Eco57I CTGAAG 3 cut(s) 957, 1137, 1421
Eco88I CYCGRG 2 cut(s) 2047, 2286
EcoO109I RGGNCCY 1 cut(s) 1869
EcoRII CCWGG 5 cut(s) 832, 966, 1318, 1557, 2382
EcoT14I CCWWGG 1 cut(s) 1482
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 2 cut(s) 1129, 2390
ErhI CCWWGG 1 cut(s) 1482
Esp3I CGTCTC 1 cut(s) 2368
FaqI GGGAC 3 cut(s) 1823, 1855, 1895
FauNDI CATATG 2 cut(s) 246, 1189
FblI GTMKAC 2 cut(s) 1224, 1470
FokI GGATG 9 cut(s) 347, 581, 716, 932, 983, 1021, 1259, 1644, 1906
FriOI GRGCYC 2 cut(s) 1129, 2390
FspBI CTAG 7 cut(s) 134, 647, 779, 1454, 1628, 1880, 2105
GlaI GCGC 1 cut(s) 2058
HaeIII GGCC 6 cut(s) 32, 911, 966, 1693, 1930, 2407
HapII CCGG 5 cut(s) 739, 930, 963, 2020, 2372
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 2059
Hin6I GCGC 1 cut(s) 2057
HinP1I GCGC 1 cut(s) 2057
HincII GTYRAC 3 cut(s) 104, 1551, 2014
HindII GTYRAC 3 cut(s) 104, 1551, 2014
HinfI GANTC 7 cut(s) 14, 406, 638, 1182, 1748, 2198, 2272
HpaI GTTAAC 2 cut(s) 1551, 2014
HpaII CCGG 5 cut(s) 739, 930, 963, 2020, 2372
HphI GGTGA 7 cut(s) 218, 548, 919, 953, 1631, 1876, 1957
Hpy166II GTNNAC 8 cut(s) 104, 448, 815, 1225, 1341, 1471, 1551, 2014
Hpy8I GTNNAC 8 cut(s) 104, 448, 815, 1225, 1341, 1471, 1551, 2014
HpyAV CCTTC 7 cut(s) 898, 932, 1686, 1750, 2113, 2188, 2311
HpyCH4III ACNGT 7 cut(s) 206, 309, 420, 445, 730, 1077, 1174
HpyCH4IV ACGT 5 cut(s) 20, 106, 478, 1291, 1763
HpyF3I CTNAG 7 cut(s) 528, 632, 786, 1158, 1314, 2166, 2276
HpySE526I ACGT 5 cut(s) 20, 106, 478, 1291, 1763
HspAI GCGC 1 cut(s) 2057
KspAI GTTAAC 2 cut(s) 1551, 2014
Kzo9I GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
LguI GCTCTTC 2 cut(s) 1257, 2395
LmnI GCTCC 3 cut(s) 115, 166, 2218
LweI GCATC 6 cut(s) 166, 217, 772, 1237, 1708, 2244
MaeI CTAG 7 cut(s) 134, 647, 779, 1454, 1628, 1880, 2105
MaeII ACGT 5 cut(s) 20, 106, 478, 1291, 1763
MaeIII GTNAC 5 cut(s) 437, 571, 690, 763, 941
MalI GATC 5 cut(s) 645, 1391, 1418, 2176, 2335
MbiI CCGCTC 1 cut(s) 1006
MboI GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
MfeI CAATTG 1 cut(s) 1653
MflI RGATCY 3 cut(s) 1389, 2174, 2333
MhlI GDGCHC 5 cut(s) 817, 1129, 1531, 2223, 2390
MlsI TGGCCA 1 cut(s) 32
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 3 cut(s) 400, 1757, 2207
MmeI TCCRAC 4 cut(s) 361, 1776, 2181, 2196
Mox20I TGGCCA 1 cut(s) 32
Mph1103I ATGCAT 1 cut(s) 624
MscI TGGCCA 1 cut(s) 32
MseI TTAA 7 cut(s) 753, 1035, 1358, 1533, 1550, 1839, 2013
MslI CAYNNNNRTG 3 cut(s) 704, 956, 2144
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 5 cut(s) 739, 930, 963, 2020, 2372
MspR9I CCNGG 7 cut(s) 739, 834, 968, 1320, 1559, 2373, 2384
MunI CAATTG 1 cut(s) 1653
Mva1269I GAATGC 3 cut(s) 1354, 1984, 2034
MvaI CCWGG 5 cut(s) 834, 968, 1320, 1559, 2384
MvnI CGCG 2 cut(s) 100, 660
NciI CCSGG 2 cut(s) 739, 2373
NdeI CATATG 2 cut(s) 246, 1189
NdeII GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
NlaIV GGNNCC 5 cut(s) 1044, 1590, 1871, 2151, 2176
NmuCI GTSAC 4 cut(s) 571, 690, 763, 941
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 1 cut(s) 1329
OliI CACNNNNGTG 2 cut(s) 704, 2144
PaeR7I CTCGAG 1 cut(s) 2286
PagI TCATGA 4 cut(s) 73, 682, 957, 2098
PaqCI CACCTGC 1 cut(s) 185
PciI ACATGT 1 cut(s) 1325
PciSI GCTCTTC 2 cut(s) 1257, 2395
PcsI WCGNNNNNNNCGW 1 cut(s) 1297
PctI GAATGC 3 cut(s) 1354, 1984, 2034
PfeI GAWTC 4 cut(s) 14, 638, 1182, 2272
PleI GAGTC 3 cut(s) 400, 1756, 2206
PpsI GAGTC 3 cut(s) 400, 1756, 2206
PpuMI RGGWCCY 1 cut(s) 1869
PscI ACATGT 1 cut(s) 1325
Psp1406I AACGTT 2 cut(s) 106, 1291
Psp5II RGGWCCY 1 cut(s) 1869
Psp6I CCWGG 5 cut(s) 832, 966, 1318, 1557, 2382
PspGI CCWGG 5 cut(s) 832, 966, 1318, 1557, 2382
PspN4I GGNNCC 5 cut(s) 1044, 1590, 1871, 2151, 2176
PspPI GGNCC 5 cut(s) 578, 1398, 1588, 1692, 1869
PspPPI RGGWCCY 1 cut(s) 1869
PspXI VCTCGAGB 1 cut(s) 2286
PstI CTGCAG 3 cut(s) 196, 901, 2146
PstNI CAGNNNCTG 2 cut(s) 185, 424
PsuI RGATCY 3 cut(s) 1389, 2174, 2333
RsaI GTAC 7 cut(s) 551, 615, 1340, 1355, 1508, 1769, 1970
RsaNI GTAC 7 cut(s) 550, 614, 1339, 1354, 1507, 1768, 1969
RseI CAYNNNNRTG 3 cut(s) 704, 956, 2144
SapI GCTCTTC 2 cut(s) 1257, 2395
SaqAI TTAA 7 cut(s) 753, 1035, 1358, 1533, 1550, 1839, 2013
Sau3AI GATC 5 cut(s) 643, 1389, 1416, 2174, 2333
Sau96I GGNCC 5 cut(s) 578, 1398, 1588, 1692, 1869
ScaI AGTACT 1 cut(s) 615
SchI GAGTC 3 cut(s) 400, 1757, 2207
ScrFI CCNGG 7 cut(s) 739, 834, 968, 1320, 1559, 2373, 2384
SduI GDGCHC 5 cut(s) 817, 1129, 1531, 2223, 2390
SfaNI GCATC 6 cut(s) 166, 217, 772, 1237, 1708, 2244
SfcI CTRYAG 4 cut(s) 192, 344, 897, 2142
Sfr274I CTCGAG 1 cut(s) 2286
SinI GGWCC 4 cut(s) 578, 1398, 1588, 1869
SlaI CTCGAG 1 cut(s) 2286
SmiMI CAYNNNNRTG 3 cut(s) 704, 956, 2144
SmlI CTYRAG 2 cut(s) 2286, 2401
SmoI CTYRAG 2 cut(s) 2286, 2401
SsiI CCGC 6 cut(s) 164, 742, 1006, 1546, 2153, 2261
SspI AATATT 1 cut(s) 1492
SspMI CTAG 7 cut(s) 134, 647, 779, 1454, 1628, 1880, 2105
StyD4I CCNGG 7 cut(s) 737, 832, 966, 1318, 1557, 2371, 2382
StyI CCWWGG 1 cut(s) 1482
TaaI ACNGT 7 cut(s) 206, 309, 420, 445, 730, 1077, 1174
TaiI ACGT 5 cut(s) 23, 109, 481, 1294, 1766
TaqI TCGA 3 cut(s) 355, 1197, 2287
TatI WGTACW 3 cut(s) 613, 1338, 1767
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 4 cut(s) 14, 638, 1182, 2272
Tru1I TTAA 7 cut(s) 753, 1035, 1358, 1533, 1550, 1839, 2013
Tru9I TTAA 7 cut(s) 753, 1035, 1358, 1533, 1550, 1839, 2013
TseFI GTSAC 4 cut(s) 571, 690, 763, 941
Tsp45I GTSAC 4 cut(s) 571, 690, 763, 941
TspDTI ATGAA 9 cut(s) 62, 104, 263, 639, 699, 911, 946, 1232, 1377
TspGWI ACGGA 1 cut(s) 716
VneI GTGCAC 1 cut(s) 813
VpaK11BI GGWCC 4 cut(s) 578, 1398, 1588, 1869
XapI RAATTY 4 cut(s) 69, 272, 828, 1733
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 1 cut(s) 1329
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XhoI CTCGAG 1 cut(s) 2286
XmiI GTMKAC 2 cut(s) 1224, 1470
XspI CTAG 7 cut(s) 134, 647, 779, 1454, 1628, 1880, 2105
ZrmI AGTACT 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.