Rroxscaffold_5G00372400

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002001
Physical Location & Seq
Reverse (-)
53833959 .. 53842576
8618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372400.1

Sequence Viewer

Length: 3915 bp
ATGGAGAATAGAGGAAACAGTGAAGCTATTAAGCTGGACAAGACGACGACGACATTGGAACCCGACAATAGCAGTGGTGGGGGATTATTTGTTCCTGGGAAAGATCGAGTTGTGTATAGACCTCCCGAGAGGAAATCTCTCTTAGGTTTAGATGTCCTTGCCATTGCAAAACGGGAAGAGTCTAAGGTTGAGGGTGGATTCAAAACCCCAAGAGAAAGAGTTGCATCGGTTGTCGCATCTCTAGAGGAGGAGGAAAACTCCGAGTCTGGGATAATAGATGAAACAGGGAGTGATATATCCACTGGTGTTCGCAGTCATTCCAAGAGGCGATATCGTGGAATATCTGCAAGTGATACACCTCGGACAGAAAGTACGGTAACGGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGTGAACGTTTTCGGACAGATGTTTTGGCCACTCCTAGCGGAAGTTCCCACAGTGTTAGGTCTCAGAGTTCATATAATAGGGATGATCGTGACAGAAGAGATCACAAGGGTAGGTCTGACAGGGACGACAATGATAGTGAGAGAAGAGATTACCAAGATAGTAGTAGGGGCGATAGTTGGAGGGAGAGAAAAAGATATGGCAGTGATAGTAAGGATTATTATGGTCGAAGAGAAGAACGTGGTCGATATGAACAAGAATATGGTGGAGAATACGAAAGAAAGCGGGGTAGATACGAAGGTTCAAAGAGGACACCTGGCCGCTCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGACACCACGACGTGATGGTTACTCTAATACCAGTAGGCGTCATCAACCATCACGATCCCCTATGCTACTCGGGGCCTCACCTGATGCACGACTAGTATCTCCTTGGTTGGGTGGGAATACACCTCATTCTGGTTCTGCTGCTTCTCCATGGGATCACATATCTCCATCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCAGCTCACATTTTCTTCGGAAAATTCACAATCATTCCAGGGTGGAGAAGCATTTAATACTGATTCGGCTGGAGAACACAATTATGAAATTAGTGAAAGCATGCGTGCAGAGATGGAATATAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGAAATACAATGTATGATACGAGTGATAGCTCATCATTGTTTTTCGGAGATGATGCTTCTTTTCAAAAAAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCTCAGATGACTGCTGATAATGCGCAGTGGGAGGATCGCCAACTTTTGAGATCGGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAATTTGATGATGAAGACGAACGCAAAGTTATTCTTCTTGTACATGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCAATAAAGGATCCTACATCTGATATGGCCATAATATCACGCAAAGGGTCTACTTTGGTTAGGGAAATCCATGAGAAGCAAAGTTCTAACAAATCACGCCAACGTTTTTGGGAGCTTGCTGGTTCCAAACTTGGGGATATCCTTGGTGTTGAGAAAACTGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGACGCTAAGTTTGCCCAACATATGAAGAATGGGGAAGCTGTCAGTGATTTTGCAAAGTCAAAAACCCTCTCACAGCAACGGCAGTATTTGCCCATATATTCTGTGAGGGATGATTTGTTGCAGGTGATTCGAGAAAATCAAGTTATCGTTGTTGTTGGAGAAACCGGTTCTGGAAAGACAACTCAACTGACACAGTATCTGCATGAAGATGGATACACGGTAAATGGTATAGTAGGTTGCACTCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAGGAAATGGAGACCGAACTTGGTGATAAAGTCGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTTCTTTTGCGCGAAACTCTAAGGGATTCTGATCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTGTTTGGAATTTTAAAAAAAGTGGTTGCCCAACGTCGTGATTTCAAGCTCATTGTGACCTCTGCAACTCTAAATGCTCAAAAGTTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCATGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGGGACATCCTCATCTTCATGACTGGCCAAGATGAGATTGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTATCTCCTCCTCTAATAAAGCGGTGCCAAAACTGTTGATACTTCCTATATATTCACAGCTGCCTGCTGACCTGCAAGCGAAGATTTTCCAAAGAGCTGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCGGAGACGTCTCTGACTGTCGATGGAATCTTTTACGTCATTGACACAGGTTATGGCAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGTGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACGGAGAATGCATACCTGAATGAAATGCTCCCGAGTCCTGTGCCGGAAATCCAGAGGACTAACCTTGGTAATGTGGTCTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGATCCACCTCCCCAAGATAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTGGGTGCTCTTAACAATGTGGGCGGCTTGACAGACCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTTTTGATGGGTGCTGAGCTGGGATGCTTGGACGAGGTTTTGACAATTGTGTCCATGCTCTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATCGGGCAGAGGAGAGTGATGCTGCAAGAGAGAAATTCTCTATTCCTGAATCCGATCACTTGACGTTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCACTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTTGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTGGGGCCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTGTTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACAGCTATGGAGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAAGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAACAAGCGCTCTTCTACATATTTGAGGCCAAAGAAACTCGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1304

Amino Acids

147.24

Weight (kDa)

6.11

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 616 - 764 2.1e-08 DEAD/DEAH box helicase
Helicase_C PF00271 811 - 940 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1005 - 1030 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1031 - 1090 1.7e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1148 - 1225 4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1840
AasI GACNNNNNNGTC 1 cut(s) 3160
AatII GACGTC 1 cut(s) 2669
Acc16I TGCGCA 1 cut(s) 1338
Acc36I ACCTGC 3 cut(s) 1663, 1840, 2601
AccB1I GGYRCC 1 cut(s) 2545
AccBSI CCGCTC 2 cut(s) 744, 2509
AccI GTMKAC 2 cut(s) 1556, 2727
AccII CGCG 1 cut(s) 2127
AciI CCGC 9 cut(s) 462, 706, 742, 2509, 2543, 2660, 2807, 3048, 3662
AclI AACGTT 3 cut(s) 430, 1609, 3276
AclWI GGATC 9 cut(s) 828, 939, 1356, 1511, 1524, 2969, 2982, 3366, 3825
AcoI YGGCCR 5 cut(s) 450, 739, 1468, 1533, 2467
AcsI RAATTY 6 cut(s) 1039, 1400, 2220, 2302, 3236, 3849
AcuI CTGAAG 1 cut(s) 2640
AcyI GRCGYC 2 cut(s) 817, 2666
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 9 cut(s) 373, 407, 1384, 1440, 2102, 2319, 2366, 2843, 3623
AfeI AGCGCT 1 cut(s) 3875
AflII CTTAAG 1 cut(s) 2939
AflIII ACRYGT 1 cut(s) 2828
AgeI ACCGGT 1 cut(s) 1892
AhlI ACTAGT 1 cut(s) 871
AjiI CACGTC 2 cut(s) 791, 2209
AjnI CCWGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
AjuI GAANNNNNNNTTGG 2 cut(s) 2979, 3011
AleI CACNNNNGTG 2 cut(s) 2207, 3641
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 2 cut(s) 3034, 3556
Alw26I GTCTC 9 cut(s) 417, 489, 773, 1320, 2021, 2199, 2657, 2673, 3721
AlwI GGATC 9 cut(s) 828, 939, 1356, 1511, 1524, 2969, 2982, 3366, 3825
AlwNI CAGNNNCTG 2 cut(s) 1688, 1927
Ama87I CYCGRG 3 cut(s) 125, 848, 2875
Aor51HI AGCGCT 1 cut(s) 3875
ApaI GGGCCC 1 cut(s) 3675
ApoI RAATTY 6 cut(s) 1039, 1400, 2220, 2302, 3236, 3849
ArsI GACNNNNNNTTYG 6 cut(s) 430, 462, 1721, 1753, 2198, 2230
AsiGI ACCGGT 1 cut(s) 1892
Asp700I GAANNNNTTC 3 cut(s) 432, 2606, 2952
AspLEI GCGC 3 cut(s) 1339, 2127, 3876
AspS9I GGNCC 9 cut(s) 852, 1011, 2081, 2819, 3091, 3195, 3325, 3671, 3672
AsuHPI GGTGA 7 cut(s) 437, 849, 1712, 1864, 2051, 2422, 3379
AsuII TTCGAA 1 cut(s) 1270
AvaI CYCGRG 3 cut(s) 125, 848, 2875
AvaII GGWCC 4 cut(s) 1011, 2819, 3091, 3325
BaeGI GKGCMC 1 cut(s) 3675
BaeI ACNNNNGTAYC 6 cut(s) 706, 739, 1374, 1407, 3287, 3320
BalI TGGCCA 3 cut(s) 452, 1535, 2469
BamHI GGATCC 2 cut(s) 1516, 2974
BanI GGYRCC 1 cut(s) 2545
BanII GRGCYC 2 cut(s) 2632, 3675
BbsI GAAGAC 4 cut(s) 1419, 1734, 3401, 3749
Bbv12I GWGCWC 2 cut(s) 3034, 3556
BceAI ACGGC 1 cut(s) 1823
BcgI CGANNNNNNTGC 4 cut(s) 1838, 1872, 2866, 2900
BciT130I CCWGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
BciVI GTATCC 2 cut(s) 965, 1934
BcoDI GTCTC 9 cut(s) 417, 489, 773, 1320, 2021, 2199, 2657, 2673, 3721
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 7 cut(s) 242, 459, 872, 2736, 3360, 3383, 3483
BfmI CTRYAG 1 cut(s) 3639
BfoI RGCGCY 1 cut(s) 3877
BfrI CTTAAG 1 cut(s) 2939
BfuAI ACCTGC 3 cut(s) 1663, 1840, 2601
BfuI GTATCC 2 cut(s) 965, 1934
BglII AGATCT 2 cut(s) 983, 3387
BlpI GCTNAGC 1 cut(s) 3126
Bme1390I CCNGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
Bme18I GGWCC 4 cut(s) 1011, 2819, 3091, 3325
BmeT110I CYCGRG 3 cut(s) 125, 848, 2875
BmgBI CACGTC 2 cut(s) 791, 2209
BmgT120I GGNCC 9 cut(s) 852, 1011, 2081, 2819, 3091, 3195, 3325, 3671, 3672
BmrFI CCNGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
BmrI ACTGGG 3 cut(s) 760, 2088, 3531
BmuI ACTGGG 3 cut(s) 760, 2088, 3531
BpiI GAAGAC 4 cut(s) 1419, 1734, 3401, 3749
BpmI CTGGAG 4 cut(s) 933, 1107, 2424, 3561
Bpu1102I GCTNAGC 1 cut(s) 3126
Bpu14I TTCGAA 1 cut(s) 1270
BpuEI CTTGAG 2 cut(s) 2915, 3768
BsaAI YACGTR 1 cut(s) 962
BsaBI GATNNNNATC 2 cut(s) 976, 2145
BsaHI GRCGYC 2 cut(s) 817, 2666
BsaI GGTCTC 3 cut(s) 489, 2021, 2199
BsaWI WCCGGW 1 cut(s) 1892
BsaXI ACNNNNNCTCC 6 cut(s) 1438, 1468, 2422, 2452, 3638, 3668
Bse118I RCCGGY 1 cut(s) 1892
Bse1I ACTGG 9 cut(s) 307, 755, 810, 950, 2083, 2470, 2821, 3320, 3526
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 2 cut(s) 976, 2145
BseBI CCWGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
BseJI GATNNNNATC 2 cut(s) 976, 2145
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 8 cut(s) 500, 1313, 1331, 1377, 1656, 3117, 3186, 3764
BseNI ACTGG 9 cut(s) 307, 755, 810, 950, 2083, 2470, 2821, 3320, 3526
BseRI GAGGAG 7 cut(s) 260, 263, 791, 1682, 2518, 2521, 3227
BseSI GKGCMC 1 cut(s) 3675
BseYI CCCAGC 3 cut(s) 2798, 3130, 3375
BsgI GTGCAG 3 cut(s) 1143, 1412, 2421
Bsh1236I CGCG 1 cut(s) 2127
BshNI GGYRCC 1 cut(s) 2545
BshTI ACCGGT 1 cut(s) 1892
BsiHKAI GWGCWC 2 cut(s) 3034, 3556
BsiHKCI CYCGRG 3 cut(s) 125, 848, 2875
BsiSI CCGG 2 cut(s) 1893, 2888
BslFI GGGAC 5 cut(s) 560, 997, 2459, 3326, 3338
BsmAI GTCTC 9 cut(s) 417, 489, 773, 1320, 2021, 2199, 2657, 2673, 3721
BsmBI CGTCTC 2 cut(s) 2657, 2673
BsmFI GGGAC 5 cut(s) 560, 997, 2459, 3326, 3338
BsmI GAATGC 1 cut(s) 2857
Bso31I GGTCTC 3 cut(s) 489, 2021, 2199
BsoBI CYCGRG 3 cut(s) 125, 848, 2875
Bsp119I TTCGAA 1 cut(s) 1270
Bsp120I GGGCCC 1 cut(s) 3671
Bsp1286I GDGCHC 4 cut(s) 2632, 3034, 3556, 3675
Bsp1407I TGTACA 3 cut(s) 1438, 2364, 2841
Bsp1720I GCTNAGC 1 cut(s) 3126
Bsp19I CCATGG 1 cut(s) 926
BspACI CCGC 9 cut(s) 462, 706, 742, 2509, 2543, 2660, 2807, 3048, 3662
BspCNI CTCAG 8 cut(s) 499, 1312, 1330, 1378, 1657, 3118, 3185, 3765
BspFNI CGCG 1 cut(s) 2127
BspHI TCATGA 2 cut(s) 2460, 3595
BspMAI CTGCAG 1 cut(s) 3643
BspMI ACCTGC 3 cut(s) 1663, 1840, 2601
BspPI GGATC 9 cut(s) 828, 939, 1356, 1511, 1524, 2969, 2982, 3366, 3825
BspQI GCTCTTC 2 cut(s) 2760, 3883
BspT104I TTCGAA 1 cut(s) 1270
BspT107I GGYRCC 1 cut(s) 2545
BspTI CTTAAG 1 cut(s) 2939
BspTNI GGTCTC 3 cut(s) 489, 2021, 2199
BsrBI CCGCTC 2 cut(s) 744, 2509
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 1892
BsrGI TGTACA 3 cut(s) 1438, 2364, 2841
BsrI ACTGG 9 cut(s) 307, 755, 810, 950, 2083, 2470, 2821, 3320, 3526
BssAI RCCGGY 1 cut(s) 1892
BssNAI GTATAC 1 cut(s) 2728
BssNI GRCGYC 2 cut(s) 817, 2666
BssT1I CCWWGG 6 cut(s) 881, 926, 1648, 1976, 2908, 3061
Bst1107I GTATAC 1 cut(s) 2728
Bst2UI CCWGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
Bst6I CTCTTC 9 cut(s) 171, 514, 562, 646, 1267, 2760, 3719, 3732, 3883
BstACI GRCGYC 2 cut(s) 817, 2666
BstAFI CTTAAG 1 cut(s) 2939
BstAPI GCANNNNNTGC 2 cut(s) 1688, 1816
BstAUI TGTACA 3 cut(s) 1438, 2364, 2841
BstBAI YACGTR 1 cut(s) 962
BstBI TTCGAA 1 cut(s) 1270
BstDSI CCRYGG 1 cut(s) 926
BstFNI CGCG 1 cut(s) 2127
BstH2I RGCGCY 1 cut(s) 3877
BstHHI GCGC 3 cut(s) 1339, 2127, 3876
BstMAI GTCTC 9 cut(s) 417, 489, 773, 1320, 2021, 2199, 2657, 2673, 3721
BstNI CCWGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
BstNSI RCATGY 4 cut(s) 1120, 2832, 3341, 3628
BstSCI CCNGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
BstSFI CTRYAG 1 cut(s) 3639
BstSLI GKGCMC 1 cut(s) 3675
BstUI CGCG 1 cut(s) 2127
BstV2I GAAGAC 4 cut(s) 1419, 1734, 3401, 3749
BstX2I RGATCY 6 cut(s) 983, 1516, 2974, 3371, 3387, 3830
BstXI CCANNNNNNTGG 2 cut(s) 2798, 3643
BstYI RGATCY 6 cut(s) 983, 1516, 2974, 3371, 3387, 3830
BstZ17I GTATAC 1 cut(s) 2728
BsuI GTATCC 2 cut(s) 965, 1934
BtgI CCRYGG 1 cut(s) 926
BtgZI GCGATG 1 cut(s) 3690
BtrI CACGTC 2 cut(s) 791, 2209
BtsI GCAGTG 8 cut(s) 79, 631, 1346, 3293, 3556, 3635, 3636, 3648
BveI ACCTGC 3 cut(s) 1663, 1840, 2601
CaiI CAGNNNCTG 2 cut(s) 1688, 1927
CciI TCATGA 2 cut(s) 2460, 3595
CfoI GCGC 3 cut(s) 1339, 2127, 3876
Cfr10I RCCGGY 1 cut(s) 1892
Cfr13I GGNCC 9 cut(s) 852, 1011, 2081, 2819, 3091, 3195, 3325, 3671, 3672
CseI GACGC 3 cut(s) 806, 954, 1739
CsiI ACCWGGT 1 cut(s) 1000
Csp6I GTAC 9 cut(s) 372, 406, 1383, 1439, 2101, 2318, 2365, 2842, 3622
CspAI ACCGGT 1 cut(s) 1892
CspCI CAANNNNNGTGG 6 cut(s) 55, 90, 1965, 2000, 3639, 3674
CviQI GTAC 9 cut(s) 372, 406, 1383, 1439, 2101, 2318, 2365, 2842, 3622
DraI TTTAAA 1 cut(s) 2226
DraIII CACNNNGTG 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 3160
DseDI GACNNNNNNGTC 1 cut(s) 3160
EaeI YGGCCR 5 cut(s) 450, 739, 1468, 1533, 2467
Eam1104I CTCTTC 9 cut(s) 171, 514, 562, 646, 1267, 2760, 3719, 3732, 3883
EarI CTCTTC 9 cut(s) 171, 514, 562, 646, 1267, 2760, 3719, 3732, 3883
EciI GGCGGA 1 cut(s) 3677
Eco130I CCWWGG 6 cut(s) 881, 926, 1648, 1976, 2908, 3061
Eco24I GRGCYC 2 cut(s) 2632, 3675
Eco31I GGTCTC 3 cut(s) 489, 2021, 2199
Eco32I GATATC 2 cut(s) 332, 1645
Eco47I GGWCC 4 cut(s) 1011, 2819, 3091, 3325
Eco47III AGCGCT 1 cut(s) 3875
Eco57I CTGAAG 1 cut(s) 2640
Eco88I CYCGRG 3 cut(s) 125, 848, 2875
EcoO109I RGGNCCY 3 cut(s) 852, 1011, 3671
EcoRII CCWGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
EcoRV GATATC 2 cut(s) 332, 1645
EcoT14I CCWWGG 6 cut(s) 881, 926, 1648, 1976, 2908, 3061
EcoT22I ATGCAT 1 cut(s) 2857
EcoT38I GRGCYC 2 cut(s) 2632, 3675
ErhI CCWWGG 6 cut(s) 881, 926, 1648, 1976, 2908, 3061
Esp3I CGTCTC 2 cut(s) 2657, 2673
FalI AAGNNNNNCTT 2 cut(s) 1416, 1448
FaqI GGGAC 5 cut(s) 560, 997, 2459, 3326, 3338
FauI CCCGC 2 cut(s) 699, 2800
FauNDI CATATG 1 cut(s) 1749
FblI GTMKAC 2 cut(s) 1556, 2727
FriOI GRGCYC 2 cut(s) 2632, 3675
FspBI CTAG 7 cut(s) 242, 459, 872, 2736, 3360, 3383, 3483
FspI TGCGCA 1 cut(s) 1338
GlaI GCGC 3 cut(s) 1338, 2126, 3875
GsaI CCCAGC 3 cut(s) 2802, 3134, 3379
GsuI CTGGAG 4 cut(s) 933, 1107, 2424, 3561
HaeII RGCGCY 1 cut(s) 3877
HapII CCGG 2 cut(s) 1893, 2888
HgaI GACGC 3 cut(s) 806, 954, 1739
HhaI GCGC 3 cut(s) 1339, 2127, 3876
Hin1I GRCGYC 2 cut(s) 817, 2666
Hin6I GCGC 3 cut(s) 1337, 2125, 3874
HinP1I GCGC 3 cut(s) 1337, 2125, 3874
HpaII CCGG 2 cut(s) 1893, 2888
HphI GGTGA 7 cut(s) 437, 849, 1712, 1864, 2051, 2422, 3379
Hpy166II GTNNAC 6 cut(s) 428, 1557, 2318, 2728, 2844, 3280
Hpy8I GTNNAC 6 cut(s) 428, 1557, 2318, 2728, 2844, 3280
Hpy99I CGWCG 4 cut(s) 49, 52, 792, 2250
HpyAV CCTTC 8 cut(s) 713, 1163, 2390, 2963, 3484, 3610, 3685, 3808
Hsp92I GRCGYC 2 cut(s) 817, 2666
HspAI GCGC 3 cut(s) 1337, 2125, 3874
LguI GCTCTTC 2 cut(s) 2760, 3883
LmnI GCTCC 6 cut(s) 1618, 1669, 2877, 3646, 3715, 3754
MabI ACCWGGT 1 cut(s) 1000
MaeI CTAG 7 cut(s) 242, 459, 872, 2736, 3360, 3383, 3483
MaeIII GTNAC 7 cut(s) 376, 512, 797, 2074, 2266, 2934, 3348
MbiI CCGCTC 2 cut(s) 744, 2509
MfeI CAATTG 1 cut(s) 3156
MflI RGATCY 6 cut(s) 983, 1516, 2974, 3371, 3387, 3830
MhlI GDGCHC 4 cut(s) 2632, 3034, 3556, 3675
MlsI TGGCCA 3 cut(s) 452, 1535, 2469
MluNI TGGCCA 3 cut(s) 452, 1535, 2469
MlyI GAGTC 3 cut(s) 188, 272, 2887
MmeI TCCRAC 3 cut(s) 581, 1864, 3693
Mox20I TGGCCA 3 cut(s) 452, 1535, 2469
Mph1103I ATGCAT 1 cut(s) 2857
MroXI GAANNNNTTC 3 cut(s) 432, 2606, 2952
MscI TGGCCA 3 cut(s) 452, 1535, 2469
MseI TTAA 8 cut(s) 30, 1071, 2225, 2355, 2940, 3036, 3342, 3510
MslI CAYNNNNRTG 5 cut(s) 1098, 1935, 2207, 2459, 3641
Msp20I TGGCCA 3 cut(s) 452, 1535, 2469
MspA1I CMGCKG 2 cut(s) 2581, 3379
MspCI CTTAAG 1 cut(s) 2939
MspI CCGG 2 cut(s) 1893, 2888
MspR9I CCNGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
MunI CAATTG 1 cut(s) 3156
Mva1269I GAATGC 1 cut(s) 2857
MvaI CCWGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
MvnI CGCG 1 cut(s) 2127
NcoI CCATGG 1 cut(s) 926
NdeI CATATG 1 cut(s) 1749
NmeAIII GCCGAG 2 cut(s) 1496, 3885
NmuCI GTSAC 3 cut(s) 512, 2266, 2934
NsbI TGCGCA 1 cut(s) 1338
NsiI ATGCAT 1 cut(s) 2857
NspI RCATGY 4 cut(s) 1120, 2832, 3341, 3628
NspV TTCGAA 1 cut(s) 1270
OliI CACNNNNGTG 2 cut(s) 2207, 3641
PaeI GCATGC 1 cut(s) 1120
PagI TCATGA 2 cut(s) 2460, 3595
PaqCI CACCTGC 1 cut(s) 1840
PciI ACATGT 1 cut(s) 2828
PciSI GCTCTTC 2 cut(s) 2760, 3883
PctI GAATGC 1 cut(s) 2857
PdmI GAANNNNTTC 3 cut(s) 432, 2606, 2952
PfeI GAWTC 8 cut(s) 198, 1079, 1855, 2141, 2685, 3251, 3695, 3769
PfoI TCCNGGA 2 cut(s) 1461, 2821
PinAI ACCGGT 1 cut(s) 1892
PleI GAGTC 3 cut(s) 187, 271, 2886
PpsI GAGTC 3 cut(s) 187, 271, 2886
Ppu21I YACGTR 1 cut(s) 962
PpuMI RGGWCCY 1 cut(s) 1011
PscI ACATGT 1 cut(s) 2828
Psp1406I AACGTT 3 cut(s) 430, 1609, 3276
Psp5II RGGWCCY 1 cut(s) 1011
Psp6I CCWGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
PspFI CCCAGC 3 cut(s) 2798, 3130, 3375
PspGI CCWGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
PspOMI GGGCCC 1 cut(s) 3671
PspPI GGNCC 9 cut(s) 852, 1011, 2081, 2819, 3091, 3195, 3325, 3671, 3672
PspPPI RGGWCCY 1 cut(s) 1011
PstI CTGCAG 1 cut(s) 3643
PstNI CAGNNNCTG 2 cut(s) 1688, 1927
PsuI RGATCY 6 cut(s) 983, 1516, 2974, 3371, 3387, 3830
PvuII CAGCTG 2 cut(s) 2581, 3379
RsaI GTAC 9 cut(s) 373, 407, 1384, 1440, 2102, 2319, 2366, 2843, 3623
RsaNI GTAC 9 cut(s) 372, 406, 1383, 1439, 2101, 2318, 2365, 2842, 3622
RseI CAYNNNNRTG 5 cut(s) 1098, 1935, 2207, 2459, 3641
SapI GCTCTTC 2 cut(s) 2760, 3883
SaqAI TTAA 8 cut(s) 30, 1071, 2225, 2355, 2940, 3036, 3342, 3510
Sau96I GGNCC 9 cut(s) 852, 1011, 2081, 2819, 3091, 3195, 3325, 3671, 3672
SchI GAGTC 3 cut(s) 188, 272, 2887
ScrFI CCNGG 9 cut(s) 96, 738, 1002, 1055, 1463, 2337, 2442, 2823, 3861
SduI GDGCHC 4 cut(s) 2632, 3034, 3556, 3675
SexAI ACCWGGT 1 cut(s) 1000
SfcI CTRYAG 1 cut(s) 3639
SfuI TTCGAA 1 cut(s) 1270
SinI GGWCC 4 cut(s) 1011, 2819, 3091, 3325
SmiMI CAYNNNNRTG 5 cut(s) 1098, 1935, 2207, 2459, 3641
SmlI CTYRAG 3 cut(s) 2930, 2939, 3783
SmoI CTYRAG 3 cut(s) 2930, 2939, 3783
SpeI ACTAGT 1 cut(s) 871
SphI GCATGC 1 cut(s) 1120
SsiI CCGC 9 cut(s) 462, 706, 742, 2509, 2543, 2660, 2807, 3048, 3662
SspI AATATT 2 cut(s) 2656, 2995
SspMI CTAG 7 cut(s) 242, 459, 872, 2736, 3360, 3383, 3483
StyD4I CCNGG 9 cut(s) 94, 736, 1000, 1053, 1461, 2335, 2440, 2821, 3859
StyI CCWWGG 6 cut(s) 881, 926, 1648, 1976, 2908, 3061
TaqI TCGA 7 cut(s) 106, 649, 667, 1270, 1858, 2679, 3307
TaqII GACCGA 1 cut(s) 2045
TatI WGTACW 5 cut(s) 1438, 2100, 2364, 2841, 3621
TauI GCSGC 2 cut(s) 744, 3051
TfiI GAWTC 8 cut(s) 198, 1079, 1855, 2141, 2685, 3251, 3695, 3769
Tru1I TTAA 8 cut(s) 30, 1071, 2225, 2355, 2940, 3036, 3342, 3510
Tru9I TTAA 8 cut(s) 30, 1071, 2225, 2355, 2940, 3036, 3342, 3510
TseFI GTSAC 3 cut(s) 512, 2266, 2934
Tsp45I GTSAC 3 cut(s) 512, 2266, 2934
TspGWI ACGGA 2 cut(s) 395, 2861
Vha464I CTTAAG 1 cut(s) 2939
VpaK11BI GGWCC 4 cut(s) 1011, 2819, 3091, 3325
XapI RAATTY 6 cut(s) 1039, 1400, 2220, 2302, 3236, 3849
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 4 cut(s) 1120, 2832, 3341, 3628
XmiI GTMKAC 2 cut(s) 1556, 2727
XmnI GAANNNNTTC 3 cut(s) 432, 2606, 2952
XspI CTAG 7 cut(s) 242, 459, 872, 2736, 3360, 3383, 3483
ZraI GACGTC 1 cut(s) 2667
Zsp2I ATGCAT 1 cut(s) 2857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.