AT5G13010

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002001
Physical Location & Seq
Forward (+)
4122400 .. 4129005
6606 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13010.1

Sequence Viewer

Length: 3768 bp
ATGGGGGTTGATCCATTCAAAACTACGGAAACGTTAGAAGCCGACAAGGAAACCAATGGTGGTGTCCCTGTGAAGGATAAATTGACTTTTAAAGCTCCTGAGAGAAAGTCCCGTTTAGGTTTGGATGCAAGGGCAATTGAGAAAAAGGATAATGCCAAGACTGAGGGCGAGTTTAAGGTTCCGAAGAAGTCAGCAATATCTGTTACATCATCGTTGGATGAAGAAGATAAATCTGATGTATCAGGATTAGATTTTGGAACAGAAAACACTCGACCTGTCCATTCCAGCAGGCGATATAGAGAGAAGTCTTCAAGATCTCAGTCTGCACAAGAAAGTACCGTGACTACAGAGAATGCTGGAACTTCAGATATCTCCATAACTCCAAGGACTTTATCTTGCACATCTAGTTATGAAAGAGGTGGTAGCAATAGGCATCGAGAAGAACATAGGCGTGACAGAAGTGAAACTCCGCGGTCAAGACAGAGAAACACTTATGATGAGATGGATCACTACCGACGGAGGGAATCTTATCGCCAATCTGACCGAGACTATCACGGAGAAAAGCGTAGGAGATACAATAGCGATTGGAGGACTCCAGGTAGGTCAGACTGGGATGATGGACAGGACGAATGGGAACGTAGTCCTCACGGAGACAGAGGCTCTAGTTACAGCAGGCGACCTCAACCTTCTCCATCACCCATGTTAGCTGCAGCTTCACCTGATGCTCGTTTAGCCTCCCCGTGGCTGGATACACCACGTTCAACTATGTCTTCTGCTTCTCCATGGGATATGGGTGCACCTTCTCCTATTCCAATTCGGGCTTCTGGATCATCTATCAGATCCTCAAGCTCTAGGTATGGTGGAAGATCCAATCAGCTTGCATATTCTAGGGAAGGTGATCTGACAAATGAGGGGCATTCAGATGAGGATAGATCGCAAGGAGCTGAAGAATTTAAACATGAGATCACAGAAACAATGCGTGTGGAAATGGAATATCAGTCAGATCGTGCATGGTATGATACAGACGAAGGGAACTCACTGTTTGATGCGGACAGTGCATCCTTTTTTCTTGGAGATGATGCTTCTCTACAGAAGAAGGAAACTGAACTGGCAAAGAGACTGGTTAGAAGAGACGGTAGCAAAATGTCACTCGCTCAGAGTAAAAAATATTCTCAGCTCAATGCGGATAATGCTCAATGGGAAGACCGCCAGCTTCTCAGATCTGGAGCTGTTAGAGGCACAGAAGTGCAGACTGAGTTTGATAGCGAAGAAGAACGGAAAGCAATTCTTCTTGTACATGATACGAAGCCTCCTTTCCTTGATGGAAGAGTCGTTTACACAAAGCAAGCAGAGCCAGTAATGCCTGTAAAGGATCCCACATCAGACATGGCTATAATTTCACGAAAAGGCTCAGGTCTTGTCAAAGAAATTCGGGAGAAACAAAGTGCGAATAAGTCACGACAGCGATTCTGGGAGCTTGCAGGTTCTAATCTTGGTAATATCCTTGGTATTGAAAAATCAGCCGAGCAGATTGATGCCGATACTGCTGTAGTTGGTGACGACGGTGAAGTAGATTTTAAAGGTGAGGCTAAATTTGCACAACATATGAAGAAGGGAGAAGCTGTGAGTGAATTTGCCATGTCAAAGACCATGGCAGAGCAACGGCAGTATCTTCCCATATTTTCTGTTAGAGATGAACTATTGCAGGTAATAAGAGAAAACCAGGTGATAGTGGTGGTTGGCGAAACTGGTTCGGGAAAGACCACTCAACTTACACAGTATCTTCATGAGGATGGTTACACTATAAATGGTATAGTTGGTTGCACCCAACCAAGGCGTGTAGCAGCCATGAGTGTTGCAAAGAGAGTTAGTGAAGAGATGGAAACAGAGTTGGGCGATAAAATTGGTTATGCAATTCGTTTTGAAGATGTAACTGGTCCAAACACTGTTATCAAGTACATGACCGATGGAGTACTACTGAGAGAGACCCTCAAAGATTCTGACCTGGATAAGTATCGTGTGGTGGTGATGGATGAAGCGCATGAAAGGTCACTCAACACAGACGTCCTTTTTGGAATACTGAAAAAAGTTGTGGCTCGGCGTCGTGATTTCAAGCTGATAGTCACGTCAGCAACCCTTAATGCTCAAAAGTTTTCCAATTTCTTTGGGAGTGTTCCTATCTTCAACATTCCTGGAAGGACTTTCCCTGTCAATATTCTCTACTCTAAAACTCCTTGTGAAGACTATGTTGAAGCTGCTGTTAAACAGGCAATGACGATTCACATAACAAGCCCACCTGGGGACATTCTCATTTTTATGACCGGGCAAGATGAGATTGAGGCAGCGTGCTTTTCACTAAAGGAGAGAATGGAACAGCTCGTATCATCTTCCAGTAGAGAAATAACGAACCTACTCATTCTCCCAATATACTCTCAGTTACCTGCTGACTTGCAAGCAAAGATATTCCAGAAACCAGAAGATGGAGCCCGTAAATGCATTGTTGCCACCAATATTGCTGAAACATCATTGACAGTCGATGGAATATATTATGTGATTGACACTGGGTATGGAAAGATGAAGGTTTTCAATCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCCATAAGTCGTGCAGCCTCTGATCAGCGTGCTGGAAGAGCTGGAAGGACAGGGCCAGGAACATGTTACAGGTTGTATACTGAGAGTGCTTATTTGAATGAGATGTTGCCAAGTCCTGTGCCAGAGATTCAGCGGACGAATCTGGGTAATGTTGTGTTGTTGTTGAAGTCATTGAAAATAGACAACTTGCTAGATTTTGATTTCATGGATCCACCTCCGCAAGAGAACATCCTTAACTCTATGTACCAGCTTTGGGTGTTGGGTGCTCTCAACAATGTTGGAGGATTAACCGACCTGGGGTGGAAGATGGTGGAGTTCCCATTGGATCCTCCTCTTGCAAAGATGCTTTTAATGGGTGAACGGCTTGATTGCATAGATGAGGTTCTGACGATCGTGTCAATGCTTTCAGTACCTTCAGTGTTCTTCAGACCTAAAGAAAGAGCAGAAGAGAGCGACGCTGCAAGGGAGAAGTTTTTTGTGCCGGAATCGGATCATCTGACGCTGCTGAACGTATATCAGCAGTGGAAAGAGCATGACTATAGAGGAGACTGGTGCAATGACCATTACCTACAAGTGAAAGGTTTGAGAAAAGCTAGAGAGGTAAGATCCCAGCTTCTGGATATCCTCAAGCAACTGAAAATTGAACTCAGGTCGTGTGGACCGGATTGGGATATTGTGAGAAAAGCCATCTGTTCAGCGTATTTCCACAACTCAGCTAGATTAAAAGGTGTTGGGGAGTATGTGAACTGCAGAACCGGGATGCCTTGCCATTTGCACCCTAGCAGTGCACTGTACGGTCTGGGATACACACCGGACTATGTGGTGTACCATGAACTGATCTTGACCACAAAGGAGTACATGCAGTGTGCGACATCGGTTGAGCCGCATTGGCTGGCTGAGTTAGGGCCTATGTTCTTCTCGGTCAAGGACTCGGATACATCGATGTTGGAGCATAAAAAAAAGCAGAAGGAAGAGAAATCAGGAATGGAGGAAGAGATGGAGAAACTGAGAAGAGATCAGGTGGAGTCAGAGCTGAGAAGCAAAGAGAGAGAGAGAAAAAAAAGGGCAAAGCAGCAGCAACAGATTTCAGGCCCTGGCTTGAAAAAGGGCACCACTTTCCTCAGGCCTAAGAAGCTTGGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1255

Amino Acids

141.86

Weight (kDa)

6.23

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 566 - 716 2.3e-08 DEAD/DEAH box helicase
Helicase_C PF00271 762 - 892 2.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 957 - 982 5.1e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 983 - 1042 1e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1100 - 1177 6.7e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3016
AatII GACGTC 1 cut(s) 2067
Acc36I ACCTGC 3 cut(s) 1472, 1696, 2449
AccB1I GGYRCC 1 cut(s) 3731
AccB7I CCANNNNNTGG 2 cut(s) 2480, 3238
AccI GTMKAC 1 cut(s) 2699
AccII CGCG 1 cut(s) 472
AciI CCGC 8 cut(s) 470, 472, 1049, 1184, 1207, 2755, 2840, 3506
AclI AACGTT 1 cut(s) 32
AcsI RAATTY 4 cut(s) 950, 1428, 1592, 1631
AcuI CTGAAG 4 cut(s) 348, 966, 3021, 3031
AcyI GRCGYC 2 cut(s) 2064, 2101
AfaI GTAC 9 cut(s) 337, 1296, 1958, 1974, 2867, 3033, 3416, 3449, 3479
AflIII ACRYGT 1 cut(s) 2684
AjiI CACGTC 1 cut(s) 2127
AjnI CCWGG 8 cut(s) 595, 1722, 2004, 2191, 2296, 2677, 2916, 3715
AjuI GAANNNNNNNTTGG 2 cut(s) 805, 837
AleI CACNNNNGTG 1 cut(s) 1244
Alw21I GWGCWC 3 cut(s) 799, 2890, 3412
Alw26I GTCTC 6 cut(s) 540, 645, 1111, 1125, 1979, 3162
Alw44I GTGCAC 2 cut(s) 795, 3408
AlwNI CAGNNNCTG 3 cut(s) 2642, 3238, 3716
AoxI GGCC 4 cut(s) 2675, 3527, 3712, 3746
ApaLI GTGCAC 2 cut(s) 795, 3408
ApoI RAATTY 4 cut(s) 950, 1428, 1592, 1631
ArsI GACNNNNNNTTYG 2 cut(s) 2054, 2086
Asp700I GAANNNNTTC 1 cut(s) 2582
AspLEI GCGC 1 cut(s) 2041
AspS9I GGNCC 5 cut(s) 1937, 2675, 3281, 3527, 3713
AsuC2I CCSGG 2 cut(s) 2323, 3379
AsuHPI GGTGA 9 cut(s) 687, 708, 908, 1568, 1577, 1595, 1738, 2038, 2990
AvaII GGWCC 2 cut(s) 1937, 3281
AxyI CCTNAGG 1 cut(s) 3743
BaeGI GKGCMC 3 cut(s) 799, 3412, 3734
BaeI ACNNNNGTAYC 4 cut(s) 1533, 1566, 1652, 1685
BamHI GGATCC 3 cut(s) 1372, 2830, 2947
BanI GGYRCC 1 cut(s) 3731
BanII GRGCYC 1 cut(s) 2488
BbsI GAAGAC 4 cut(s) 300, 762, 1209, 2247
Bbv12I GWGCWC 3 cut(s) 799, 2890, 3412
BceAI ACGGC 2 cut(s) 1679, 2999
BciT130I CCWGG 8 cut(s) 597, 1724, 2006, 2193, 2298, 2679, 2918, 3717
BciVI GTATCC 3 cut(s) 742, 3419, 3550
BclI TGATCA 1 cut(s) 2644
BcnI CCSGG 2 cut(s) 2323, 3379
BcoDI GTCTC 6 cut(s) 540, 645, 1111, 1125, 1979, 3162
BfaI CTAG 9 cut(s) 405, 663, 852, 888, 2592, 2813, 3216, 3339, 3402
BfmI CTRYAG 6 cut(s) 345, 708, 1088, 1548, 3160, 3370
BfuAI ACCTGC 3 cut(s) 1472, 1696, 2449
BfuI GTATCC 3 cut(s) 742, 3419, 3550
BglI GCCNNNNNGGC 1 cut(s) 3511
BglII AGATCT 2 cut(s) 314, 1220
BmcAI AGTACT 1 cut(s) 1974
Bme18I GGWCC 2 cut(s) 1937, 3281
BmgBI CACGTC 1 cut(s) 2127
BmgT120I GGNCC 5 cut(s) 1937, 2675, 3281, 3527, 3713
BmiI GGNNCC 6 cut(s) 180, 1374, 2485, 2832, 2949, 3733
BmrI ACTGGG 2 cut(s) 619, 2571
BmuI ACTGGG 2 cut(s) 619, 2571
BpiI GAAGAC 4 cut(s) 300, 762, 1209, 2247
BplI GAGNNNNNCTC 2 cut(s) 1974, 2006
BpmI CTGGAG 2 cut(s) 579, 1245
Bpu10I CCTNAGC 1 cut(s) 1411
BpuEI CTTGAG 2 cut(s) 829, 3233
BpuMI CCSGG 2 cut(s) 2323, 3379
Bsa29I ATCGAT 1 cut(s) 3563
BsaBI GATNNNNATC 1 cut(s) 2013
BsaHI GRCGYC 2 cut(s) 2064, 2101
BsaI GGTCTC 1 cut(s) 1979
BsaWI WCCGGW 2 cut(s) 3283, 3433
BsaXI ACNNNNNCTCC 6 cut(s) 1294, 1324, 1608, 1638, 2403, 2433
Bse1I ACTGG 9 cut(s) 614, 1113, 1125, 1355, 1753, 1939, 2391, 2566, 3176
Bse21I CCTNAGG 1 cut(s) 3743
Bse3DI GCAATG 2 cut(s) 2277, 3184
Bse8I GATNNNNATC 1 cut(s) 2013
BseBI CCWGG 8 cut(s) 597, 1724, 2006, 2193, 2298, 2679, 2918, 3717
BseCI ATCGAT 1 cut(s) 3563
BseGI GGATG 9 cut(s) 130, 223, 619, 1058, 1798, 2038, 2611, 2850, 3387
BseJI GATNNNNATC 1 cut(s) 2013
BseMI GCAATG 2 cut(s) 2277, 3184
BseNI ACTGG 9 cut(s) 614, 1113, 1125, 1355, 1753, 1939, 2391, 2566, 3176
BseRI GAGGAG 2 cut(s) 2943, 3180
BseSI GKGCMC 3 cut(s) 799, 3412, 3734
BseYI CCCAGC 1 cut(s) 3231
BsgI GTGCAG 3 cut(s) 309, 1268, 2655
Bsh1236I CGCG 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 3015
BshFI GGCC 4 cut(s) 2677, 3529, 3714, 3748
BshNI GGYRCC 1 cut(s) 3731
BshVI ATCGAT 1 cut(s) 3563
BsiEI CGRYCG 1 cut(s) 3015
BsiHKAI GWGCWC 3 cut(s) 799, 2890, 3412
BsiSI CCGG 5 cut(s) 2322, 3104, 3284, 3378, 3434
BslFI GGGAC 3 cut(s) 50, 94, 2315
BsmAI GTCTC 6 cut(s) 540, 645, 1111, 1125, 1979, 3162
BsmBI CGTCTC 1 cut(s) 1125
BsmFI GGGAC 3 cut(s) 50, 94, 2315
BsmI GAATGC 2 cut(s) 358, 916
BsnI GGCC 4 cut(s) 2677, 3529, 3714, 3748
Bso31I GGTCTC 1 cut(s) 1979
Bsp1286I GDGCHC 5 cut(s) 799, 2488, 2890, 3412, 3734
Bsp1407I TGTACA 1 cut(s) 1294
Bsp19I CCATGG 2 cut(s) 782, 1650
BspACI CCGC 8 cut(s) 470, 472, 1049, 1184, 1207, 2755, 2840, 3506
BspANI GGCC 4 cut(s) 2677, 3529, 3714, 3748
BspDI ATCGAT 1 cut(s) 3563
BspFNI CGCG 1 cut(s) 472
BspHI TCATGA 1 cut(s) 1786
BspLI GGNNCC 6 cut(s) 180, 1374, 2485, 2832, 2949, 3733
BspMAI CTGCAG 2 cut(s) 712, 3374
BspMI ACCTGC 3 cut(s) 1472, 1696, 2449
BspQI GCTCTTC 2 cut(s) 2616, 2653
BspT107I GGYRCC 1 cut(s) 3731
BspTNI GGTCTC 1 cut(s) 1979
BsrDI GCAATG 2 cut(s) 2277, 3184
BsrGI TGTACA 1 cut(s) 1294
BsrI ACTGG 9 cut(s) 614, 1113, 1125, 1355, 1753, 1939, 2391, 2566, 3176
BssNAI GTATAC 1 cut(s) 2700
BssNI GRCGYC 2 cut(s) 2064, 2101
BssT1I CCWWGG 5 cut(s) 383, 782, 1504, 1650, 1832
Bst1107I GTATAC 1 cut(s) 2700
Bst2UI CCWGG 8 cut(s) 597, 1724, 2006, 2193, 2298, 2679, 2918, 3717
Bst6I CTCTTC 9 cut(s) 1123, 1321, 1869, 2616, 2653, 3063, 3588, 3609, 3628
BstACI GRCGYC 2 cut(s) 2064, 2101
BstAUI TGTACA 1 cut(s) 1294
BstDSI CCRYGG 4 cut(s) 470, 740, 782, 1650
BstF5I GGATG 9 cut(s) 130, 223, 619, 1058, 1798, 2038, 2611, 2850, 3387
BstFNI CGCG 1 cut(s) 472
BstHHI GCGC 1 cut(s) 2041
BstMAI GTCTC 6 cut(s) 540, 645, 1111, 1125, 1979, 3162
BstMCI CGRYCG 1 cut(s) 3015
BstNI CCWGG 8 cut(s) 597, 1724, 2006, 2193, 2298, 2679, 2918, 3717
BstNSI RCATGY 2 cut(s) 2688, 3484
BstSFI CTRYAG 6 cut(s) 345, 708, 1088, 1548, 3160, 3370
BstSLI GKGCMC 3 cut(s) 799, 3412, 3734
BstUI CGCG 1 cut(s) 472
BstV2I GAAGAC 4 cut(s) 300, 762, 1209, 2247
BstX2I RGATCY 8 cut(s) 314, 839, 866, 1220, 1372, 2830, 2947, 3227
BstYI RGATCY 8 cut(s) 314, 839, 866, 1220, 1372, 2830, 2947, 3227
BstZ17I GTATAC 1 cut(s) 2700
Bsu15I ATCGAT 1 cut(s) 3563
Bsu36I CCTNAGG 1 cut(s) 3743
BsuI GTATCC 3 cut(s) 742, 3419, 3550
BsuRI GGCC 4 cut(s) 2677, 3529, 3714, 3748
BsuTUI ATCGAT 1 cut(s) 3563
BtgI CCRYGG 4 cut(s) 470, 740, 782, 1650
BtrI CACGTC 1 cut(s) 2127
BtsCI GGATG 9 cut(s) 130, 223, 619, 1058, 1798, 2038, 2611, 2850, 3387
BtsI GCAGTG 3 cut(s) 3149, 3412, 3491
BtsIMutI CAGTG 9 cut(s) 1037, 1060, 1944, 2559, 3045, 3149, 3410, 3412, 3491
BveI ACCTGC 3 cut(s) 1472, 1696, 2449
CaiI CAGNNNCTG 3 cut(s) 2642, 3238, 3716
CciI TCATGA 1 cut(s) 1786
CfoI GCGC 1 cut(s) 2041
Cfr13I GGNCC 5 cut(s) 1937, 2675, 3281, 3527, 3713
Cfr42I CCGCGG 1 cut(s) 473
ClaI ATCGAT 1 cut(s) 3563
CseI GACGC 3 cut(s) 2090, 3086, 3130
CsiI ACCWGGT 1 cut(s) 1722
Csp6I GTAC 9 cut(s) 336, 1295, 1957, 1973, 2866, 3032, 3415, 3448, 3478
CspCI CAANNNNNGTGG 4 cut(s) 963, 998, 2494, 2529
CviQI GTAC 9 cut(s) 336, 1295, 1957, 1973, 2866, 3032, 3415, 3448, 3478
DraI TTTAAA 3 cut(s) 91, 955, 1579
DrdI GACNNNNNNGTC 1 cut(s) 3016
DseDI GACNNNNNNGTC 1 cut(s) 3016
Eam1104I CTCTTC 9 cut(s) 1123, 1321, 1869, 2616, 2653, 3063, 3588, 3609, 3628
EarI CTCTTC 9 cut(s) 1123, 1321, 1869, 2616, 2653, 3063, 3588, 3609, 3628
Eco130I CCWWGG 5 cut(s) 383, 782, 1504, 1650, 1832
Eco147I AGGCCT 1 cut(s) 3748
Eco24I GRGCYC 1 cut(s) 2488
Eco31I GGTCTC 1 cut(s) 1979
Eco32I GATATC 2 cut(s) 370, 3244
Eco47I GGWCC 2 cut(s) 1937, 3281
Eco57I CTGAAG 4 cut(s) 348, 966, 3021, 3031
Eco81I CCTNAGG 1 cut(s) 3743
EcoO109I RGGNCCY 2 cut(s) 3527, 3713
EcoRII CCWGG 8 cut(s) 595, 1722, 2004, 2191, 2296, 2677, 2916, 3715
EcoRV GATATC 2 cut(s) 370, 3244
EcoT14I CCWWGG 5 cut(s) 383, 782, 1504, 1650, 1832
EcoT22I ATGCAT 1 cut(s) 2498
EcoT38I GRGCYC 1 cut(s) 2488
ErhI CCWWGG 5 cut(s) 383, 782, 1504, 1650, 1832
Esp3I CGTCTC 1 cut(s) 1125
FalI AAGNNNNNCTT 2 cut(s) 1272, 1304
FaqI GGGAC 3 cut(s) 50, 94, 2315
FauNDI CATATG 1 cut(s) 1605
FbaI TGATCA 1 cut(s) 2644
FblI GTMKAC 1 cut(s) 2699
FokI GGATG 9 cut(s) 137, 230, 626, 1045, 1805, 2045, 2618, 2837, 3394
FriOI GRGCYC 1 cut(s) 2488
FspBI CTAG 9 cut(s) 405, 663, 852, 888, 2592, 2813, 3216, 3339, 3402
GlaI GCGC 1 cut(s) 2040
GsaI CCCAGC 1 cut(s) 3235
GsuI CTGGAG 2 cut(s) 579, 1245
HaeIII GGCC 4 cut(s) 2677, 3529, 3714, 3748
HapII CCGG 5 cut(s) 2322, 3104, 3284, 3378, 3434
HgaI GACGC 3 cut(s) 2090, 3086, 3130
HhaI GCGC 1 cut(s) 2041
Hin1I GRCGYC 2 cut(s) 2064, 2101
Hin6I GCGC 1 cut(s) 2039
HinP1I GCGC 1 cut(s) 2039
HindIII AAGCTT 1 cut(s) 3755
HpaII CCGG 5 cut(s) 2322, 3104, 3284, 3378, 3434
HphI GGTGA 9 cut(s) 687, 708, 908, 1568, 1577, 1595, 1738, 2038, 2990
Hpy166II GTNNAC 8 cut(s) 797, 1336, 2700, 2981, 3281, 3367, 3410, 3448
Hpy8I GTNNAC 8 cut(s) 797, 1336, 2700, 2981, 3281, 3367, 3410, 3448
Hpy99I CGWCG 4 cut(s) 519, 1565, 2106, 3080
HpyCH4IV ACGT 6 cut(s) 32, 637, 757, 2064, 2126, 3132
HpySE526I ACGT 6 cut(s) 32, 637, 757, 2064, 2126, 3132
Hsp92I GRCGYC 2 cut(s) 2064, 2101
HspAI GCGC 1 cut(s) 2039
Ksp22I TGATCA 1 cut(s) 2644
KspI CCGCGG 1 cut(s) 473
LguI GCTCTTC 2 cut(s) 2616, 2653
LmnI GCTCC 6 cut(s) 100, 941, 1226, 1474, 2483, 3571
MabI ACCWGGT 1 cut(s) 1722
MaeI CTAG 9 cut(s) 405, 663, 852, 888, 2592, 2813, 3216, 3339, 3402
MaeII ACGT 6 cut(s) 32, 637, 757, 2064, 2126, 3132
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 8 cut(s) 314, 839, 866, 1220, 1372, 2830, 2947, 3227
MhlI GDGCHC 5 cut(s) 799, 2488, 2890, 3412, 3734
MlyI GAGTC 4 cut(s) 586, 1338, 3545, 3656
MmeI TCCRAC 3 cut(s) 195, 2881, 3549
Mph1103I ATGCAT 1 cut(s) 2498
MroXI GAANNNNTTC 1 cut(s) 2582
MslI CAYNNNNRTG 5 cut(s) 450, 921, 1244, 1791, 2315
MspA1I CMGCKG 2 cut(s) 472, 2755
MspI CCGG 5 cut(s) 2322, 3104, 3284, 3378, 3434
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 2 cut(s) 358, 916
MvaI CCWGG 8 cut(s) 597, 1724, 2006, 2193, 2298, 2679, 2918, 3717
MvnI CGCG 1 cut(s) 472
NciI CCSGG 2 cut(s) 2323, 3379
NcoI CCATGG 2 cut(s) 782, 1650
NdeI CATATG 1 cut(s) 1605
NlaIV GGNNCC 6 cut(s) 180, 1374, 2485, 2832, 2949, 3733
NmeAIII GCCGAG 2 cut(s) 1549, 2077
NmuCI GTSAC 7 cut(s) 340, 452, 1146, 1455, 1556, 2049, 2122
NsiI ATGCAT 1 cut(s) 2498
NspI RCATGY 2 cut(s) 2688, 3484
OliI CACNNNNGTG 1 cut(s) 1244
PagI TCATGA 1 cut(s) 1786
PceI AGGCCT 1 cut(s) 3748
PciI ACATGT 1 cut(s) 2684
PciSI GCTCTTC 2 cut(s) 2616, 2653
PcsI WCGNNNNNNNCGW 1 cut(s) 3560
PctI GAATGC 2 cut(s) 358, 916
PdmI GAANNNNTTC 1 cut(s) 2582
PfeI GAWTC 7 cut(s) 524, 1467, 1997, 2278, 2749, 2761, 3107
PflMI CCANNNNNTGG 2 cut(s) 2480, 3238
PfoI TCCNGGA 1 cut(s) 2191
Ple19I CGATCG 1 cut(s) 3015
PleI GAGTC 4 cut(s) 586, 1337, 3545, 3655
PpsI GAGTC 4 cut(s) 586, 1337, 3545, 3655
PscI ACATGT 1 cut(s) 2684
Psp1406I AACGTT 1 cut(s) 32
Psp6I CCWGG 8 cut(s) 595, 1722, 2004, 2191, 2296, 2677, 2916, 3715
PspFI CCCAGC 1 cut(s) 3231
PspGI CCWGG 8 cut(s) 595, 1722, 2004, 2191, 2296, 2677, 2916, 3715
PspN4I GGNNCC 6 cut(s) 180, 1374, 2485, 2832, 2949, 3733
PspPI GGNCC 5 cut(s) 1937, 2675, 3281, 3527, 3713
PstI CTGCAG 2 cut(s) 712, 3374
PstNI CAGNNNCTG 3 cut(s) 2642, 3238, 3716
PsuI RGATCY 8 cut(s) 314, 839, 866, 1220, 1372, 2830, 2947, 3227
PvuI CGATCG 1 cut(s) 3015
RsaI GTAC 9 cut(s) 337, 1296, 1958, 1974, 2867, 3033, 3416, 3449, 3479
RsaNI GTAC 9 cut(s) 336, 1295, 1957, 1973, 2866, 3032, 3415, 3448, 3478
RseI CAYNNNNRTG 5 cut(s) 450, 921, 1244, 1791, 2315
SacII CCGCGG 1 cut(s) 473
SapI GCTCTTC 2 cut(s) 2616, 2653
Sau96I GGNCC 5 cut(s) 1937, 2675, 3281, 3527, 3713
ScaI AGTACT 1 cut(s) 1974
SchI GAGTC 4 cut(s) 586, 1338, 3545, 3656
SduI GDGCHC 5 cut(s) 799, 2488, 2890, 3412, 3734
SexAI ACCWGGT 1 cut(s) 1722
SfcI CTRYAG 6 cut(s) 345, 708, 1088, 1548, 3160, 3370
Sfr303I CCGCGG 1 cut(s) 473
SgrBI CCGCGG 1 cut(s) 473
SinI GGWCC 2 cut(s) 1937, 3281
SmiMI CAYNNNNRTG 5 cut(s) 450, 921, 1244, 1791, 2315
SmlI CTYRAG 2 cut(s) 844, 3248
SmoI CTYRAG 2 cut(s) 844, 3248
SseBI AGGCCT 1 cut(s) 3748
SsiI CCGC 8 cut(s) 470, 472, 1049, 1184, 1207, 2755, 2840, 3506
SspI AATATT 3 cut(s) 1169, 2215, 2512
SspMI CTAG 9 cut(s) 405, 663, 852, 888, 2592, 2813, 3216, 3339, 3402
StuI AGGCCT 1 cut(s) 3748
StyI CCWWGG 5 cut(s) 383, 782, 1504, 1650, 1832
TaiI ACGT 6 cut(s) 35, 640, 760, 2067, 2129, 3135
TaqI TCGA 4 cut(s) 271, 436, 2535, 3563
TaqII GACCGA 3 cut(s) 558, 1979, 3532
TatI WGTACW 4 cut(s) 1294, 1956, 1972, 3477
TauI GCSGC 1 cut(s) 3508
TfiI GAWTC 7 cut(s) 524, 1467, 1997, 2278, 2749, 2761, 3107
TscAI CASTG 9 cut(s) 1044, 1060, 1951, 2566, 3045, 3149, 3412, 3417, 3491
TseFI GTSAC 7 cut(s) 340, 452, 1146, 1455, 1556, 2049, 2122
Tsp45I GTSAC 7 cut(s) 340, 452, 1146, 1455, 1556, 2049, 2122
TspGWI ACGGA 5 cut(s) 41, 532, 570, 663, 1291
TspRI CASTG 9 cut(s) 1044, 1060, 1951, 2566, 3045, 3149, 3412, 3417, 3491
Van91I CCANNNNNTGG 2 cut(s) 2480, 3238
VneI GTGCAC 2 cut(s) 795, 3408
VpaK11BI GGWCC 2 cut(s) 1937, 3281
XapI RAATTY 4 cut(s) 950, 1428, 1592, 1631
XceI RCATGY 2 cut(s) 2688, 3484
XcmI CCANNNNNNNNNTGG 1 cut(s) 1384
XmiI GTMKAC 1 cut(s) 2699
XmnI GAANNNNTTC 1 cut(s) 2582
XspI CTAG 9 cut(s) 405, 663, 852, 888, 2592, 2813, 3216, 3339, 3402
ZraI GACGTC 1 cut(s) 2065
ZrmI AGTACT 1 cut(s) 1974
Zsp2I ATGCAT 1 cut(s) 2498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.