Rroxscaffold_1G00056820

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002001
Physical Location & Seq
Reverse (-)
78766200 .. 78770267
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00056820.1

Sequence Viewer

Length: 3261 bp
ATGGTGAAATCGGAACTCGAGAAGAGCGGAGGAGGTGGAGGATTACATGTTCGCGGGACGGAGAGAGTTGTTTTCAGGCCGCCGGAGAAGAAGAAATTAGGGTTTGATGGAGGTGTCAGGAGATATAAGGGTAGGTATGATGATGGGGATGGTAGTGAAAGAAGAGATTACAGAGATGGTGGTGGTGGTAGTAGGAGTAGGGTGAGAGGAAGATATGAGGAAGATGGATATGGTGGAGATTATGGAAGAAAGAAGAGTAGATATGAACGTTCGAAGAGGGGAGCTCAATCTGTTGCTTCTGCCTGGGATTGTATCTCTTACTCTCCGGTTCAGATACGGGCTTCTGGACAGTCAGTCAAGTCTTCGGGTTTGGGACGTGGTGGGAGTTCTCATCAGCTTGATAGTACGCTAGGAGAAGAGGGTGATTCGGTTGAAGGGAGATATGAGATTGGTGAGAGCATGCGTTTAGAGATGGAATATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCACAGCGGTTGATACGAGTGATAGTTCGTCTTTGTTTTATGGGGATGATGCTTTTTGTCGAAAGAAGGAAGCGGAGTTGGCGAAGAGATTGATTCGGAGAGATGGAACAAAGATGAGCCTTGCTCAGAGCAAAAGGTTGTCTCAGCGTACTGCAGATAATGCTCAGTGGGAGGATCGTCAGCTTCTGCGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAACAAAGTTATGCTTCTTGTTCATGATACAAGGCCTCCTTTCCTGGATGGGAGAGTTGTTTTTACCAAGCAAGCGGAGCCGGTTATGCCAGTAAAGGATCCCACATCTGATATGGCCATAATTTCACGCAAAGGGTCTGCTCTAGTTAGGGAAATCCATCAGAAGCAAAGTTCGAATAAATCACGTCAACGTTTTTGGGAGCTTGCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAGAAAACAGCAGAGCATATTGATGCAGATACTGCTGTTGTAGGTAAAGACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCTCAGCATATGAAGAATGGGGAGGCTGTGAGTGAATTCACAAAGTCAAAAACTATTTCCGAACAACGCCAGTATCTGCCTATATATTCTGTGCGGGATGAGTTGCTGCAGAAAATTCGAGAAAATCAGGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGAATGAAGATGGTTACACTGTGAACGGTATAGTCGGTTGCACCCAGCCAAGGCGTGTGGCAGCCATGAGTGTTGCTAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGAGAAAGTTGGCTATGCTATTCGTTTTGAGGATGTGACGGGGCCAGAAACTGTAATAAAGTACATGACTGATGGAGTACTTATGCATGAAACACTTAGAGATTCTGACCTCGACAAGTACCGTGTAGTTGTAATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATTTTGAAAAAAGTCGTCGCCCAGCGTCGTGATTTTAAGCTCATTGTGACATCTGCTACTCTTAATGCTCAGAAATTTGCAGATTTCTTTGGAGGTGCACCTATTTTTCCCATTCCTGGAAGAACATTTCCTGTACATAAGAAGTACTTGGATACCCCATGTGAAGATTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTGGCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTTGCAGAGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGGGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTTTACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGTTGTACACAGAGAGTGCCTACCTCAATGAAATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTCAAGTCTCTGAAGGTTGATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCAGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAAAATGGTGGAGTTCCCATTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTAGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCAGAGTCCGATCACCTGACATTCTATAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTACAAAAGGCTAGACAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACGTTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCGTACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCACTTTATGGTATGGGCTGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACAGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGACAATCTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAGCAAGCAGAGGGAGAGGGAAAAGAGGTATAAACAGCAGCAGCAGCAAGTCTCATTGGCGGGTTTGCACCAGGGCTCTTCTACATATCTGAAGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1086

Amino Acids

122.05

Weight (kDa)

6.59

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 394 - 546 4e-09 DEAD/DEAH box helicase
AAA_22 PF13401 402 - 535 2.3e-06 AAA domain
Helicase_C PF00271 592 - 721 1.6e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 786 - 811 6.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 812 - 871 2e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 929 - 1005 5.5e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 959
AccBSI CCGCTC 1 cut(s) 27
AccII CGCG 1 cut(s) 54
AciI CCGC 9 cut(s) 27, 54, 80, 524, 590, 836, 1177, 2993, 3206
AclI AACGTT 2 cut(s) 268, 952
AclWI GGATC 4 cut(s) 699, 854, 867, 2230
AcoI YGGCCR 3 cut(s) 876, 1810, 2217
AcsI RAATTY 7 cut(s) 1118, 1197, 1637, 1764, 2490, 2579, 2748
AcuI CTGAAG 3 cut(s) 2303, 2756, 3257
AfiI CCNNNNNNNGG 8 cut(s) 1320, 1533, 1679, 2083, 2084, 2362, 2409, 2547
AflIII ACRYGT 2 cut(s) 46, 2171
AgsI TTSAA 8 cut(s) 434, 1066, 1570, 1739, 1826, 2102, 2746, 2816
AhdI GACNNNNNGTC 1 cut(s) 353
AjiI CACGTC 2 cut(s) 377, 947
AjnI CCWGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
Alw21I GWGCWC 4 cut(s) 286, 1663, 2377, 2899
Alw26I GTCTC 6 cut(s) 663, 1364, 2000, 2283, 3064, 3202
Alw44I GTGCAC 2 cut(s) 1659, 2895
AlwI GGATC 4 cut(s) 699, 854, 867, 2230
AlwNI CAGNNNCTG 5 cut(s) 703, 1031, 1159, 1433, 2950
Ama87I CYCGRG 3 cut(s) 17, 2571, 2887
ApaLI GTGCAC 2 cut(s) 1659, 2895
ApoI RAATTY 7 cut(s) 1118, 1197, 1637, 1764, 2490, 2579, 2748
Asp700I GAANNNNTTC 2 cut(s) 2337, 2946
AspA2I CCTAGG 1 cut(s) 2078
AspS9I GGNCC 7 cut(s) 1424, 2162, 2317, 2434, 2538, 2715, 3014
AsuII TTCGAA 2 cut(s) 272, 935
AvaI CYCGRG 3 cut(s) 17, 2571, 2887
AvaII GGWCC 4 cut(s) 2317, 2434, 2715, 3014
AvrII CCTAGG 1 cut(s) 2078
AxyI CCTNAGG 1 cut(s) 2404
BaeGI GKGCMC 2 cut(s) 1663, 2899
BalI TGGCCA 2 cut(s) 878, 2219
BamHI GGATCC 1 cut(s) 859
BanII GRGCYC 4 cut(s) 286, 1975, 2377, 3224
BbsI GAAGAC 2 cut(s) 354, 2744
Bbv12I GWGCWC 4 cut(s) 286, 1663, 2377, 2899
BcgI CGANNNNNNTGC 2 cut(s) 1181, 1215
BciT130I CCWGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
BciVI GTATCC 3 cut(s) 1708, 2515, 3037
BcoDI GTCTC 6 cut(s) 663, 1364, 2000, 2283, 3064, 3202
BfaI CTAG 7 cut(s) 410, 905, 2079, 2112, 2474, 2703, 2726
BfmI CTRYAG 3 cut(s) 669, 1190, 1743
BfuAI ACCTGC 1 cut(s) 959
BfuI GTATCC 3 cut(s) 1708, 2515, 3037
BlnI CCTAGG 1 cut(s) 2078
BlpI GCTNAGC 1 cut(s) 1086
BmcAI AGTACT 2 cut(s) 1461, 1709
Bme1390I CCNGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
Bme18I GGWCC 4 cut(s) 2317, 2434, 2715, 3014
BmeRI GACNNNNNGTC 1 cut(s) 353
BmeT110I CYCGRG 3 cut(s) 17, 2571, 2887
BmgBI CACGTC 2 cut(s) 377, 947
BmgT120I GGNCC 7 cut(s) 1424, 2162, 2317, 2434, 2538, 2715, 3014
BmrFI CCNGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
BmrI ACTGGG 2 cut(s) 2169, 2874
BmsI GCATC 7 cut(s) 556, 1012, 1063, 2083, 2468, 2554, 2859
BmuI ACTGGG 2 cut(s) 2169, 2874
BpiI GAAGAC 2 cut(s) 354, 2744
BplI GAGNNNNNCTC 6 cut(s) 268, 300, 625, 657, 3109, 3141
BpmI CTGGAG 1 cut(s) 1767
Bpu1102I GCTNAGC 1 cut(s) 1086
Bpu14I TTCGAA 2 cut(s) 272, 935
BpuEI CTTGAG 2 cut(s) 2258, 3111
BsaAI YACGTR 1 cut(s) 2260
BsaJI CCNNGG 6 cut(s) 303, 1319, 2078, 2251, 2328, 3217
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 4 cut(s) 781, 811, 2981, 3011
Bsc4I CCNNNNNNNGG 8 cut(s) 1320, 1533, 1679, 2083, 2084, 2362, 2409, 2547
Bse118I RCCGGY 2 cut(s) 841, 1808
Bse1I ACTGG 8 cut(s) 851, 1153, 1240, 2053, 2164, 2220, 2663, 2869
Bse21I CCTNAGG 1 cut(s) 2404
BseBI CCWGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
BseDI CCNNGG 6 cut(s) 303, 1319, 2078, 2251, 2328, 3217
BseLI CCNNNNNNNGG 8 cut(s) 1320, 1533, 1679, 2083, 2084, 2362, 2409, 2547
BseNI ACTGG 8 cut(s) 851, 1153, 1240, 2053, 2164, 2220, 2663, 2869
BseRI GAGGAG 6 cut(s) 45, 1832, 1861, 1864, 2570, 3113
BseSI GKGCMC 2 cut(s) 1663, 2899
BseYI CCCAGC 4 cut(s) 1314, 1584, 2141, 2718
BsgI GTGCAG 3 cut(s) 755, 2683, 2899
Bsh1236I CGCG 1 cut(s) 54
BsiHKAI GWGCWC 4 cut(s) 286, 1663, 2377, 2899
BsiHKCI CYCGRG 3 cut(s) 17, 2571, 2887
BsiSI CCGG 5 cut(s) 83, 326, 842, 1809, 2860
BslFI GGGAC 6 cut(s) 70, 387, 2573, 2669, 2701, 3027
BslI CCNNNNNNNGG 8 cut(s) 1320, 1533, 1679, 2083, 2084, 2362, 2409, 2547
BsmAI GTCTC 6 cut(s) 663, 1364, 2000, 2283, 3064, 3202
BsmFI GGGAC 6 cut(s) 70, 387, 2573, 2669, 2701, 3027
BsoBI CYCGRG 3 cut(s) 17, 2571, 2887
Bsp119I TTCGAA 2 cut(s) 272, 935
Bsp1286I GDGCHC 6 cut(s) 286, 1663, 1975, 2377, 2899, 3224
Bsp1407I TGTACA 2 cut(s) 1696, 2184
Bsp143I GATC 5 cut(s) 691, 707, 859, 2235, 2599
Bsp1720I GCTNAGC 1 cut(s) 1086
BspACI CCGC 9 cut(s) 27, 54, 80, 524, 590, 836, 1177, 2993, 3206
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 3 cut(s) 784, 1803, 2938
BspMAI CTGCAG 3 cut(s) 673, 1194, 1747
BspMI ACCTGC 1 cut(s) 959
BspPI GGATC 4 cut(s) 699, 854, 867, 2230
BspQI GCTCTTC 4 cut(s) 17, 2103, 2463, 3229
BspT104I TTCGAA 2 cut(s) 272, 935
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 841, 1808
BsrGI TGTACA 2 cut(s) 1696, 2184
BsrI ACTGG 8 cut(s) 851, 1153, 1240, 2053, 2164, 2220, 2663, 2869
BssAI RCCGGY 2 cut(s) 841, 1808
BssECI CCNNGG 6 cut(s) 303, 1319, 2078, 2251, 2328, 3217
BssMI GATC 5 cut(s) 691, 707, 859, 2235, 2599
BssT1I CCWWGG 3 cut(s) 1319, 2078, 2251
Bst2UI CCWGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
Bst4CI ACNGT 9 cut(s) 351, 491, 1052, 1291, 1298, 1435, 1505, 2020, 2631
BstAPI GCANNNNNTGC 3 cut(s) 677, 1031, 1082
BstAUI TGTACA 2 cut(s) 1696, 2184
BstBAI YACGTR 1 cut(s) 2260
BstBI TTCGAA 2 cut(s) 272, 935
BstC8I GCNNGC 8 cut(s) 461, 834, 966, 1929, 1941, 2148, 2217, 3152
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 5 cut(s) 694, 710, 862, 2238, 2602
BstMAI GTCTC 6 cut(s) 663, 1364, 2000, 2283, 3064, 3202
BstMBI GATC 5 cut(s) 691, 707, 859, 2235, 2599
BstMWI GCNNNNNNNGC 9 cut(s) 596, 677, 838, 847, 1031, 1082, 2147, 2518, 3190
BstNI CCWGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
BstNSI RCATGY 5 cut(s) 50, 463, 2175, 2684, 2971
BstSCI CCNGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
BstSFI CTRYAG 3 cut(s) 669, 1190, 1743
BstSLI GKGCMC 2 cut(s) 1663, 2899
BstUI CGCG 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 354, 2744
BstX2I RGATCY 2 cut(s) 859, 2235
BstXI CCANNNNNNTGG 1 cut(s) 2141
BstYI RGATCY 2 cut(s) 859, 2235
Bsu36I CCTNAGG 1 cut(s) 2404
BsuI GTATCC 3 cut(s) 1708, 2515, 3037
BtgZI GCGATG 1 cut(s) 2577
BtrI CACGTC 2 cut(s) 377, 947
BtsI GCAGTG 6 cut(s) 1752, 2671, 2899, 2978, 2991, 3002
BveI ACCTGC 1 cut(s) 959
Cac8I GCNNGC 8 cut(s) 461, 834, 966, 1929, 1941, 2148, 2217, 3152
CaiI CAGNNNCTG 5 cut(s) 703, 1031, 1159, 1433, 2950
CciI TCATGA 3 cut(s) 784, 1803, 2938
Cfr10I RCCGGY 2 cut(s) 841, 1808
Cfr13I GGNCC 7 cut(s) 1424, 2162, 2317, 2434, 2538, 2715, 3014
CseI GACGC 1 cut(s) 1577
CspCI CAANNNNNGTGG 2 cut(s) 1308, 1343
DpnI GATC 5 cut(s) 693, 709, 861, 2237, 2601
DpnII GATC 5 cut(s) 691, 707, 859, 2235, 2599
DriI GACNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 3 cut(s) 876, 1810, 2217
Eam1105I GACNNNNNGTC 1 cut(s) 353
Ecl136II GAGCTC 2 cut(s) 284, 2375
Eco130I CCWWGG 3 cut(s) 1319, 2078, 2251
Eco147I AGGCCT 1 cut(s) 796
Eco24I GRGCYC 4 cut(s) 286, 1975, 2377, 3224
Eco32I GATATC 1 cut(s) 1792
Eco47I GGWCC 4 cut(s) 2317, 2434, 2715, 3014
Eco53kI GAGCTC 2 cut(s) 284, 2375
Eco57I CTGAAG 3 cut(s) 2303, 2756, 3257
Eco81I CCTNAGG 1 cut(s) 2404
Eco88I CYCGRG 3 cut(s) 17, 2571, 2887
EcoICRI GAGCTC 2 cut(s) 284, 2375
EcoO109I RGGNCCY 2 cut(s) 2715, 3014
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
EcoRV GATATC 1 cut(s) 1792
EcoT14I CCWWGG 3 cut(s) 1319, 2078, 2251
EcoT22I ATGCAT 2 cut(s) 1470, 1985
EcoT38I GRGCYC 4 cut(s) 286, 1975, 2377, 3224
ErhI CCWWGG 3 cut(s) 1319, 2078, 2251
FalI AAGNNNNNCTT 6 cut(s) 759, 791, 784, 816, 1694, 1726
FaqI GGGAC 6 cut(s) 70, 387, 2573, 2669, 2701, 3027
FauI CCCGC 3 cut(s) 47, 1170, 3199
FauNDI CATATG 1 cut(s) 1092
FriOI GRGCYC 4 cut(s) 286, 1975, 2377, 3224
FspBI CTAG 7 cut(s) 410, 905, 2079, 2112, 2474, 2703, 2726
GsaI CCCAGC 4 cut(s) 1318, 1588, 2145, 2722
GsuI CTGGAG 1 cut(s) 1767
HapII CCGG 5 cut(s) 83, 326, 842, 1809, 2860
HgaI GACGC 1 cut(s) 1577
HincII GTYRAC 1 cut(s) 950
HindII GTYRAC 1 cut(s) 950
HinfI GANTC 7 cut(s) 425, 610, 1484, 2594, 2731, 2947, 3038
HpaII CCGG 5 cut(s) 83, 326, 842, 1809, 2860
Hpy166II GTNNAC 6 cut(s) 950, 1294, 1661, 2071, 2187, 2897
Hpy8I GTNNAC 6 cut(s) 950, 1294, 1661, 2071, 2187, 2897
Hpy99I CGWCG 2 cut(s) 1583, 1593
HpyAV CCTTC 4 cut(s) 428, 506, 577, 2278
HpyCH4III ACNGT 9 cut(s) 351, 491, 1052, 1291, 1298, 1435, 1505, 2020, 2631
HpyCH4IV ACGT 8 cut(s) 268, 376, 946, 952, 2137, 2259, 2741, 2781
HpyF10VI GCNNNNNNNGC 9 cut(s) 596, 677, 838, 847, 1031, 1082, 2147, 2518, 3190
HpySE526I ACGT 8 cut(s) 268, 376, 946, 952, 2137, 2259, 2741, 2781
KflI GGGWCCC 1 cut(s) 3014
Kzo9I GATC 5 cut(s) 691, 707, 859, 2235, 2599
LguI GCTCTTC 4 cut(s) 17, 2103, 2463, 3229
LmnI GCTCC 5 cut(s) 281, 838, 961, 2372, 2989
LweI GCATC 7 cut(s) 556, 1012, 1063, 2083, 2468, 2554, 2859
MaeI CTAG 7 cut(s) 410, 905, 2079, 2112, 2474, 2703, 2726
MaeII ACGT 8 cut(s) 268, 376, 946, 952, 2137, 2259, 2741, 2781
MaeIII GTNAC 9 cut(s) 485, 1283, 1417, 1536, 1609, 1747, 2666, 2691, 2977
MalI GATC 5 cut(s) 693, 709, 861, 2237, 2601
MbiI CCGCTC 1 cut(s) 27
MboI GATC 5 cut(s) 691, 707, 859, 2235, 2599
MfeI CAATTG 1 cut(s) 2499
MflI RGATCY 2 cut(s) 859, 2235
MhlI GDGCHC 6 cut(s) 286, 1663, 1975, 2377, 2899, 3224
MlsI TGGCCA 2 cut(s) 878, 2219
MluNI TGGCCA 2 cut(s) 878, 2219
MlyI GAGTC 2 cut(s) 2603, 3047
MmeI TCCRAC 2 cut(s) 1207, 3021
Mox20I TGGCCA 2 cut(s) 878, 2219
Mph1103I ATGCAT 2 cut(s) 1470, 1985
MroXI GAANNNNTTC 2 cut(s) 2337, 2946
MscI TGGCCA 2 cut(s) 878, 2219
MseI TTAA 5 cut(s) 1599, 1626, 2685, 2832, 2853
MslI CAYNNNNRTG 2 cut(s) 1020, 1802
Msp20I TGGCCA 2 cut(s) 878, 2219
MspA1I CMGCKG 5 cut(s) 524, 1924, 1931, 2359, 2722
MspI CCGG 5 cut(s) 83, 326, 842, 1809, 2860
MspR9I CCNGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
MunI CAATTG 1 cut(s) 2499
MvaI CCWGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 9 cut(s) 596, 677, 838, 847, 1031, 1082, 2147, 2518, 3190
NdeI CATATG 1 cut(s) 1092
NdeII GATC 5 cut(s) 691, 707, 859, 2235, 2599
NmuCI GTSAC 6 cut(s) 485, 1417, 1536, 1609, 1747, 2666
NsiI ATGCAT 2 cut(s) 1470, 1985
NspI RCATGY 5 cut(s) 50, 463, 2175, 2684, 2971
NspV TTCGAA 2 cut(s) 272, 935
PaeI GCATGC 1 cut(s) 463
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 3 cut(s) 784, 1803, 2938
PceI AGGCCT 1 cut(s) 796
PciI ACATGT 2 cut(s) 46, 2171
PciSI GCTCTTC 4 cut(s) 17, 2103, 2463, 3229
PcsI WCGNNNNNNNCGW 1 cut(s) 2143
PdmI GAANNNNTTC 2 cut(s) 2337, 2946
PfeI GAWTC 5 cut(s) 425, 610, 1484, 2731, 2947
PfoI TCCNGGA 2 cut(s) 804, 1678
PleI GAGTC 2 cut(s) 2602, 3046
PpsI GAGTC 2 cut(s) 2602, 3046
Ppu21I YACGTR 1 cut(s) 2260
PpuMI RGGWCCY 2 cut(s) 2715, 3014
PscI ACATGT 2 cut(s) 46, 2171
Psp124BI GAGCTC 2 cut(s) 286, 2377
Psp1406I AACGTT 2 cut(s) 268, 952
Psp5II RGGWCCY 2 cut(s) 2715, 3014
Psp6I CCWGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
PspFI CCCAGC 4 cut(s) 1314, 1584, 2141, 2718
PspGI CCWGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
PspPI GGNCC 7 cut(s) 1424, 2162, 2317, 2434, 2538, 2715, 3014
PspPPI RGGWCCY 2 cut(s) 2715, 3014
PsrI GAACNNNNNNTAC 2 cut(s) 2240, 2272
PstI CTGCAG 3 cut(s) 673, 1194, 1747
PstNI CAGNNNCTG 5 cut(s) 703, 1031, 1159, 1433, 2950
PsuI RGATCY 2 cut(s) 859, 2235
PvuII CAGCTG 4 cut(s) 1924, 1931, 2359, 2722
RseI CAYNNNNRTG 2 cut(s) 1020, 1802
SacI GAGCTC 2 cut(s) 286, 2377
SapI GCTCTTC 4 cut(s) 17, 2103, 2463, 3229
SaqAI TTAA 5 cut(s) 1599, 1626, 2685, 2832, 2853
Sau3AI GATC 5 cut(s) 691, 707, 859, 2235, 2599
Sau96I GGNCC 7 cut(s) 1424, 2162, 2317, 2434, 2538, 2715, 3014
ScaI AGTACT 2 cut(s) 1461, 1709
SchI GAGTC 2 cut(s) 2603, 3047
ScrFI CCNGG 7 cut(s) 304, 806, 1680, 1785, 2166, 2330, 3218
SduI GDGCHC 6 cut(s) 286, 1663, 1975, 2377, 2899, 3224
SfaNI GCATC 7 cut(s) 556, 1012, 1063, 2083, 2468, 2554, 2859
SfcI CTRYAG 3 cut(s) 669, 1190, 1743
Sfr274I CTCGAG 1 cut(s) 17
SfuI TTCGAA 2 cut(s) 272, 935
SinI GGWCC 4 cut(s) 2317, 2434, 2715, 3014
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 2 cut(s) 1020, 1802
SmlI CTYRAG 3 cut(s) 17, 2273, 3126
SmoI CTYRAG 3 cut(s) 17, 2273, 3126
SphI GCATGC 1 cut(s) 463
SseBI AGGCCT 1 cut(s) 796
SsiI CCGC 9 cut(s) 27, 54, 80, 524, 590, 836, 1177, 2993, 3206
SspI AATATT 2 cut(s) 1999, 2338
SspMI CTAG 7 cut(s) 410, 905, 2079, 2112, 2474, 2703, 2726
SstI GAGCTC 2 cut(s) 286, 2377
StuI AGGCCT 1 cut(s) 796
StyD4I CCNGG 7 cut(s) 302, 804, 1678, 1783, 2164, 2328, 3216
StyI CCWWGG 3 cut(s) 1319, 2078, 2251
TaaI ACNGT 9 cut(s) 351, 491, 1052, 1291, 1298, 1435, 1505, 2020, 2631
TaiI ACGT 8 cut(s) 271, 379, 949, 955, 2140, 2262, 2744, 2784
TaqI TCGA 7 cut(s) 18, 272, 577, 935, 1201, 1494, 2379
TatI WGTACW 6 cut(s) 1443, 1459, 1696, 1707, 2184, 2964
TauI GCSGC 2 cut(s) 82, 2995
TfiI GAWTC 5 cut(s) 425, 610, 1484, 2731, 2947
Tru1I TTAA 5 cut(s) 1599, 1626, 2685, 2832, 2853
Tru9I TTAA 5 cut(s) 1599, 1626, 2685, 2832, 2853
TseFI GTSAC 6 cut(s) 485, 1417, 1536, 1609, 1747, 2666
Tsp45I GTSAC 6 cut(s) 485, 1417, 1536, 1609, 1747, 2666
TspGWI ACGGA 1 cut(s) 74
VneI GTGCAC 2 cut(s) 1659, 2895
VpaK11BI GGWCC 4 cut(s) 2317, 2434, 2715, 3014
XapI RAATTY 7 cut(s) 1118, 1197, 1637, 1764, 2490, 2579, 2748
XceI RCATGY 5 cut(s) 50, 463, 2175, 2684, 2971
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XhoI CTCGAG 1 cut(s) 17
XmaJI CCTAGG 1 cut(s) 2078
XmnI GAANNNNTTC 2 cut(s) 2337, 2946
XspI CTAG 7 cut(s) 410, 905, 2079, 2112, 2474, 2703, 2726
ZrmI AGTACT 2 cut(s) 1461, 1709
Zsp2I ATGCAT 2 cut(s) 1470, 1985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.