Rw4G026140

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002001
Physical Location & Seq
Reverse (-)
52328763 .. 52336363
7601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026140.1

Sequence Viewer

Length: 3921 bp
ATGGAGAATAGAGGAAACAGTGAAGCTATCAAGCTGGACAAGACGACGACGACATTGGAACCCGAGAATAGCAGTGGTGGGGGATTATTTGTTCCTGGGAAAGATCGAGTTGTGTATAGACCTCCCGAGAGGAAATCTCTCTTAGGTTTAGATGTCCTTGCCATTGCAAAACGGGAAGAGTCTAAGGTTGAGGGTGGATTCAAAACCCCAAGAGAAAGAGTTGCATCGGTTGTCGCATCTTTAGAGGAGGAGGAAAACTCTGAGTCTGGTGTAATAGATGAAACAGGGAGTGATATATCCACTGGTGTACGCAGTCATTCCAAGAGACGATATCGTGGAATATCTGCAAGTGATACACCTCGGACAGAAAGTACGGTAACGGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGTGAACGTTTTCGGACAGATGTTTTGGCCACTCCTAGTGGAAGTTCCCACAGTGTTAGGTCTCAGAGTTCATATAATAGGGATGATCGTGGTAGTGACAGAAGAGATCACAAGGGTAGGTCTGACAGGGACGACAATGATAGTGAGAGAAGAGATTATCAAGATAGTAGTAGGGGCGATAGTTGGAGGGAGAGAAAAAGATATGGCAGTGATAGTAAGGATTATTATGGTCGAAGAGAAGAACGTGGTCGATATGAACAAGAATATGGAGGAGAATATGAAAGAAAGCGGGGTAGATACGAAGGTTCAAAGAGGACACCTGGCCGCTCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGACACCACGACGTGATGGTTACTCTAATACCAGTAGGCGTCATCAACCATCACGATCCCCTATGCTACTCGGGGCCTCACCTGATGCACGACTAGTATCTCCTTGGCTGGGTGGGAATACACCTCATTCTGGTTCTGCTGCTTCTCCATGGGATCACATATCTCCATCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCAGCTCACATTTTCTTCGGAAAATTCACAATCATTCCAGGGTGGAGAAGCATTTAATAATGATTCGGCTGGAGAACACAATTATGAAATTAGTGAAAGCATGCGTGCAGAGATGGAATACAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGAAATACAATGTATGATACGAGTGATAGCTCATCATTGTTTTTCGGAGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCTCAGATGACTGCTGATAATGCGCAGTGGGAGGATCGACAACTTTTGAGATCGGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAATTTGATGATGAAGACGAACGCAAAGTTATTCTTCTTGTACATGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCAATAAAGGATCCCACATCTGATATGGCCATAATATCACGCAAAGGGTCTACTTTGGTTAGGGAAATCCATGAGAAGCAAAGTTCTAACAAATCACGCCAACGTTTTTGGGAGCTCGCTGGTTCCAAACTTGGGGATATCCTTGGTGTTGAGAAAACTGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGACGCTAAGTTTGCCCAACATATGAAGAATGGGGAAGCTGTCAGTGATTTTGCAAAGTCAAAAACTCTCTCACAGCAACGGCAGTATTTGCCCATATATTCTGTGAGGGATGATTTGTTGCAGGTGATTCGAGAAAATCAAGTTATCGTTGTTGTTGGAGAAACCGGTTCTGGAAAGACAACTCAACTGACACAGTATCTGCATGAAGATGGATACACGGTAAATGGTATAGTAGGTTGCACTCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAGGAAATGGAGACCGAACTTGGTGATAAAGTCGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTTCTTTTGCGCGAAACTCTAAGGGATTCTGATCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTGTTTGGAATTTTAAAAAAAGTGGTTGCCCAACGTCGTGATTTCAAGCTCATTGTGACCTCTGCAACTCTAAATGCTCAAAAGTTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCATGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGTGACATCCTCATCTTCATGACTGGCCAAGATGAGATTGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTATCTCCTCCTCTAATAAAGCGGTGCCAAAACTGTTGATACTTCCTATATATTCACAGCTGCCTGCTGACCTGCAAGCGAAGATTTTCCAAAGAGCTGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCGGAGACGTCTCTGACTGTCGATGGAATCTTTTACGTCATTGACACAGGTTATGGCAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGTGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACGGAGAATGCATACCTGAATGAAATGCTCCCAAGTCCTGTGCCGGAAATCCAGAGGACTAACCTTGGTAATGTGGTCTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGACCCACCTCCCCAAGATAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTGGGTGCTCTTAACAACGTGGGCGGCTTGACAGACCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTTTTGATGGGTGCTGAGCTGGGATGCTTGGACGAGGTTTTGACAATTGTGTCCATGCTCTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATCGGGCAGAGGAGAGTGATGCTGCACGAGAGAAATTCTCTATTCCTGAATCCGATCACTTGACGTTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCACTGACGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTTGTTTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTGGGGCCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTGTTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACAGCTATGGAGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAAGGAGAGAGAAAAACGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAACAAGCGCTCTTCTACATATTTGAGGCCAAAGAAACTCGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1306

Amino Acids

147.4

Weight (kDa)

6.12

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 618 - 766 2.1e-08 DEAD/DEAH box helicase
Helicase_C PF00271 813 - 942 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1007 - 1032 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1033 - 1092 1.7e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1150 - 1227 4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1846
AasI GACNNNNNNGTC 1 cut(s) 3166
AatII GACGTC 1 cut(s) 2675
Acc16I TGCGCA 1 cut(s) 1344
Acc36I ACCTGC 3 cut(s) 1669, 1846, 2607
AccB1I GGYRCC 1 cut(s) 2551
AccBSI CCGCTC 2 cut(s) 750, 2515
AccI GTMKAC 2 cut(s) 1562, 2733
AccII CGCG 1 cut(s) 2133
AciI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
AclI AACGTT 3 cut(s) 430, 1615, 3282
AclWI GGATC 7 cut(s) 834, 945, 1362, 1517, 1530, 3372, 3831
AcoI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
AcsI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
AcuI CTGAAG 1 cut(s) 2646
AcyI GRCGYC 2 cut(s) 823, 2672
AdeI CACNNNGTG 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 3881
AfiI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
AflII CTTAAG 1 cut(s) 2945
AflIII ACRYGT 1 cut(s) 2834
AgeI ACCGGT 1 cut(s) 1898
AhlI ACTAGT 1 cut(s) 877
AjiI CACGTC 2 cut(s) 797, 2215
AjnI CCWGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
AjuI GAANNNNNNNTTGG 2 cut(s) 2985, 3017
AleI CACNNNNGTG 2 cut(s) 2213, 3647
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 3 cut(s) 1629, 3040, 3562
AlwI GGATC 7 cut(s) 834, 945, 1362, 1517, 1530, 3372, 3831
AlwNI CAGNNNCTG 2 cut(s) 1694, 1933
Ama87I CYCGRG 3 cut(s) 62, 125, 854
Aor51HI AGCGCT 1 cut(s) 3881
ApaI GGGCCC 1 cut(s) 3681
ApoI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
ArsI GACNNNNNNTTYG 6 cut(s) 430, 462, 1727, 1759, 2204, 2236
AsiGI ACCGGT 1 cut(s) 1898
Asp700I GAANNNNTTC 3 cut(s) 432, 2612, 2958
AspLEI GCGC 3 cut(s) 1345, 2133, 3882
AsuHPI GGTGA 8 cut(s) 437, 855, 1718, 1870, 2057, 2428, 2462, 3385
AsuII TTCGAA 1 cut(s) 1276
AvaI CYCGRG 3 cut(s) 62, 125, 854
AvaII GGWCC 5 cut(s) 1017, 2825, 2980, 3097, 3331
BaeGI GKGCMC 1 cut(s) 3681
BaeI ACNNNNGTAYC 6 cut(s) 712, 745, 1380, 1413, 3293, 3326
BalI TGGCCA 3 cut(s) 452, 1541, 2475
BamHI GGATCC 1 cut(s) 1522
BanI GGYRCC 1 cut(s) 2551
BanII GRGCYC 3 cut(s) 1629, 2638, 3681
BauI CACGAG 1 cut(s) 3234
BbsI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
Bbv12I GWGCWC 3 cut(s) 1629, 3040, 3562
BceAI ACGGC 1 cut(s) 1829
BcgI CGANNNNNNTGC 2 cut(s) 1844, 1878
BciT130I CCWGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BciVI GTATCC 2 cut(s) 971, 1940
BcuI ACTAGT 1 cut(s) 877
BfaI CTAG 6 cut(s) 459, 878, 2742, 3366, 3389, 3489
BfmI CTRYAG 1 cut(s) 3645
BfoI RGCGCY 1 cut(s) 3883
BfrI CTTAAG 1 cut(s) 2945
BfuAI ACCTGC 3 cut(s) 1669, 1846, 2607
BfuI GTATCC 2 cut(s) 971, 1940
BglII AGATCT 2 cut(s) 989, 3393
BlpI GCTNAGC 1 cut(s) 3132
Bme1390I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bme18I GGWCC 5 cut(s) 1017, 2825, 2980, 3097, 3331
BmeT110I CYCGRG 3 cut(s) 62, 125, 854
BmgBI CACGTC 2 cut(s) 797, 2215
BmrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BmrI ACTGGG 3 cut(s) 766, 2094, 3537
BmuI ACTGGG 3 cut(s) 766, 2094, 3537
BpiI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BpmI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
Bpu1102I GCTNAGC 1 cut(s) 3132
Bpu14I TTCGAA 1 cut(s) 1276
BpuEI CTTGAG 2 cut(s) 2921, 3774
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 2 cut(s) 982, 2151
BsaHI GRCGYC 2 cut(s) 823, 2672
BsaI GGTCTC 3 cut(s) 489, 2027, 2205
BsaWI WCCGGW 1 cut(s) 1898
BsaXI ACNNNNNCTCC 6 cut(s) 1444, 1474, 2428, 2458, 3644, 3674
Bsc4I CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
Bse118I RCCGGY 1 cut(s) 1898
Bse1I ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 2 cut(s) 982, 2151
BseBI CCWGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BseJI GATNNNNATC 2 cut(s) 982, 2151
BseLI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 9 cut(s) 252, 500, 1319, 1337, 1383, 1662, 3123, 3192, 3770
BseNI ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
BseRI GAGGAG 8 cut(s) 260, 263, 708, 797, 1688, 2524, 2527, 3233
BseSI GKGCMC 1 cut(s) 3681
BseYI CCCAGC 4 cut(s) 892, 2804, 3136, 3381
BsgI GTGCAG 4 cut(s) 1149, 1418, 2427, 3216
Bsh1236I CGCG 1 cut(s) 2133
BshNI GGYRCC 1 cut(s) 2551
BshTI ACCGGT 1 cut(s) 1898
BsiHKAI GWGCWC 3 cut(s) 1629, 3040, 3562
BsiHKCI CYCGRG 3 cut(s) 62, 125, 854
BsiSI CCGG 2 cut(s) 1899, 2894
BslFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BslI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BsmBI CGTCTC 3 cut(s) 319, 2663, 2679
BsmFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BsmI GAATGC 1 cut(s) 2863
Bso31I GGTCTC 3 cut(s) 489, 2027, 2205
BsoBI CYCGRG 3 cut(s) 62, 125, 854
Bsp119I TTCGAA 1 cut(s) 1276
Bsp120I GGGCCC 1 cut(s) 3677
Bsp1286I GDGCHC 5 cut(s) 1629, 2638, 3040, 3562, 3681
Bsp1407I TGTACA 3 cut(s) 1444, 2370, 2847
Bsp1720I GCTNAGC 1 cut(s) 3132
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
BspCNI CTCAG 9 cut(s) 253, 499, 1318, 1336, 1384, 1663, 3124, 3191, 3771
BspFNI CGCG 1 cut(s) 2133
BspHI TCATGA 2 cut(s) 2466, 3601
BspMAI CTGCAG 1 cut(s) 3649
BspMI ACCTGC 3 cut(s) 1669, 1846, 2607
BspPI GGATC 7 cut(s) 834, 945, 1362, 1517, 1530, 3372, 3831
BspQI GCTCTTC 2 cut(s) 2766, 3889
BspT104I TTCGAA 1 cut(s) 1276
BspT107I GGYRCC 1 cut(s) 2551
BspTI CTTAAG 1 cut(s) 2945
BspTNI GGTCTC 3 cut(s) 489, 2027, 2205
BsrBI CCGCTC 2 cut(s) 750, 2515
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 1898
BsrGI TGTACA 3 cut(s) 1444, 2370, 2847
BsrI ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
BssAI RCCGGY 1 cut(s) 1898
BssNAI GTATAC 1 cut(s) 2734
BssNI GRCGYC 2 cut(s) 823, 2672
BssSI CACGAG 1 cut(s) 3234
BssT1I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Bst1107I GTATAC 1 cut(s) 2734
Bst2BI CACGAG 1 cut(s) 3234
Bst2UI CCWGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bst6I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
BstACI GRCGYC 2 cut(s) 823, 2672
BstAFI CTTAAG 1 cut(s) 2945
BstAPI GCANNNNNTGC 2 cut(s) 1694, 1822
BstAUI TGTACA 3 cut(s) 1444, 2370, 2847
BstBAI YACGTR 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 1276
BstDSI CCRYGG 1 cut(s) 932
BstFNI CGCG 1 cut(s) 2133
BstH2I RGCGCY 1 cut(s) 3883
BstHHI GCGC 3 cut(s) 1345, 2133, 3882
BstNI CCWGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BstNSI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
BstSCI CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
BstSFI CTRYAG 1 cut(s) 3645
BstSLI GKGCMC 1 cut(s) 3681
BstUI CGCG 1 cut(s) 2133
BstV2I GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BstX2I RGATCY 4 cut(s) 989, 1522, 3377, 3393
BstXI CCANNNNNNTGG 2 cut(s) 2804, 3649
BstYI RGATCY 4 cut(s) 989, 1522, 3377, 3393
BstZ17I GTATAC 1 cut(s) 2734
BsuI GTATCC 2 cut(s) 971, 1940
BtgI CCRYGG 1 cut(s) 932
BtgZI GCGATG 1 cut(s) 3696
BtrI CACGTC 2 cut(s) 797, 2215
BtsI GCAGTG 8 cut(s) 79, 637, 1352, 3299, 3562, 3641, 3642, 3654
BveI ACCTGC 3 cut(s) 1669, 1846, 2607
CaiI CAGNNNCTG 2 cut(s) 1694, 1933
CciI TCATGA 2 cut(s) 2466, 3601
CfoI GCGC 3 cut(s) 1345, 2133, 3882
Cfr10I RCCGGY 1 cut(s) 1898
CseI GACGC 3 cut(s) 812, 960, 1745
CsiI ACCWGGT 1 cut(s) 1006
CspAI ACCGGT 1 cut(s) 1898
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 3645, 3680
DraI TTTAAA 1 cut(s) 2232
DraIII CACNNNGTG 1 cut(s) 797
DrdI GACNNNNNNGTC 1 cut(s) 3166
DseDI GACNNNNNNGTC 1 cut(s) 3166
EaeI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
Eam1104I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EarI CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EciI GGCGGA 1 cut(s) 3683
Ecl136II GAGCTC 1 cut(s) 1627
Eco130I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Eco24I GRGCYC 3 cut(s) 1629, 2638, 3681
Eco31I GGTCTC 3 cut(s) 489, 2027, 2205
Eco32I GATATC 2 cut(s) 332, 1651
Eco47I GGWCC 5 cut(s) 1017, 2825, 2980, 3097, 3331
Eco47III AGCGCT 1 cut(s) 3881
Eco53kI GAGCTC 1 cut(s) 1627
Eco57I CTGAAG 1 cut(s) 2646
Eco88I CYCGRG 3 cut(s) 62, 125, 854
EcoICRI GAGCTC 1 cut(s) 1627
EcoO109I RGGNCCY 3 cut(s) 858, 1017, 3677
EcoRII CCWGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
EcoRV GATATC 2 cut(s) 332, 1651
EcoT14I CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
EcoT22I ATGCAT 1 cut(s) 2863
EcoT38I GRGCYC 3 cut(s) 1629, 2638, 3681
ErhI CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
Esp3I CGTCTC 3 cut(s) 319, 2663, 2679
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 4 cut(s) 566, 1003, 3332, 3344
FauI CCCGC 2 cut(s) 705, 2806
FauNDI CATATG 1 cut(s) 1755
FblI GTMKAC 2 cut(s) 1562, 2733
FriOI GRGCYC 3 cut(s) 1629, 2638, 3681
FspBI CTAG 6 cut(s) 459, 878, 2742, 3366, 3389, 3489
FspI TGCGCA 1 cut(s) 1344
GlaI GCGC 3 cut(s) 1344, 2132, 3881
GsaI CCCAGC 4 cut(s) 896, 2808, 3140, 3385
GsuI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
HaeII RGCGCY 1 cut(s) 3883
HapII CCGG 2 cut(s) 1899, 2894
HgaI GACGC 3 cut(s) 812, 960, 1745
HhaI GCGC 3 cut(s) 1345, 2133, 3882
Hin1I GRCGYC 2 cut(s) 823, 2672
Hin6I GCGC 3 cut(s) 1343, 2131, 3880
HinP1I GCGC 3 cut(s) 1343, 2131, 3880
HpaII CCGG 2 cut(s) 1899, 2894
HphI GGTGA 8 cut(s) 437, 855, 1718, 1870, 2057, 2428, 2462, 3385
Hpy166II GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy8I GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy99I CGWCG 4 cut(s) 49, 52, 798, 2256
HpyAV CCTTC 9 cut(s) 719, 1169, 1240, 2396, 2969, 3490, 3616, 3691, 3814
Hsp92I GRCGYC 2 cut(s) 823, 2672
HspAI GCGC 3 cut(s) 1343, 2131, 3880
LguI GCTCTTC 2 cut(s) 2766, 3889
LmnI GCTCC 6 cut(s) 1624, 1675, 2883, 3652, 3721, 3760
MabI ACCWGGT 1 cut(s) 1006
MaeI CTAG 6 cut(s) 459, 878, 2742, 3366, 3389, 3489
MaeIII GTNAC 8 cut(s) 376, 518, 803, 2080, 2272, 2450, 2940, 3354
MbiI CCGCTC 2 cut(s) 750, 2515
MfeI CAATTG 1 cut(s) 3162
MflI RGATCY 4 cut(s) 989, 1522, 3377, 3393
MhlI GDGCHC 5 cut(s) 1629, 2638, 3040, 3562, 3681
MlsI TGGCCA 3 cut(s) 452, 1541, 2475
MluNI TGGCCA 3 cut(s) 452, 1541, 2475
MlyI GAGTC 2 cut(s) 188, 272
MmeI TCCRAC 3 cut(s) 587, 1870, 3699
Mox20I TGGCCA 3 cut(s) 452, 1541, 2475
Mph1103I ATGCAT 1 cut(s) 2863
MroXI GAANNNNTTC 3 cut(s) 432, 2612, 2958
MscI TGGCCA 3 cut(s) 452, 1541, 2475
MseI TTAA 7 cut(s) 1077, 2231, 2361, 2946, 3042, 3348, 3516
MslI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
Msp20I TGGCCA 3 cut(s) 452, 1541, 2475
MspA1I CMGCKG 2 cut(s) 2587, 3385
MspCI CTTAAG 1 cut(s) 2945
MspI CCGG 2 cut(s) 1899, 2894
MspR9I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MunI CAATTG 1 cut(s) 3162
Mva1269I GAATGC 1 cut(s) 2863
MvaI CCWGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MvnI CGCG 1 cut(s) 2133
NcoI CCATGG 1 cut(s) 932
NdeI CATATG 1 cut(s) 1755
NmeAIII GCCGAG 2 cut(s) 1502, 3891
NmuCI GTSAC 4 cut(s) 518, 2272, 2450, 2940
NsbI TGCGCA 1 cut(s) 1344
NsiI ATGCAT 1 cut(s) 2863
NspI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
NspV TTCGAA 1 cut(s) 1276
OliI CACNNNNGTG 2 cut(s) 2213, 3647
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 2 cut(s) 2466, 3601
PaqCI CACCTGC 1 cut(s) 1846
PciI ACATGT 1 cut(s) 2834
PciSI GCTCTTC 2 cut(s) 2766, 3889
PctI GAATGC 1 cut(s) 2863
PdmI GAANNNNTTC 3 cut(s) 432, 2612, 2958
PfeI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
PfoI TCCNGGA 2 cut(s) 1467, 2827
PinAI ACCGGT 1 cut(s) 1898
PleI GAGTC 2 cut(s) 187, 271
PpsI GAGTC 2 cut(s) 187, 271
Ppu21I YACGTR 1 cut(s) 968
PpuMI RGGWCCY 1 cut(s) 1017
PscI ACATGT 1 cut(s) 2834
Psp124BI GAGCTC 1 cut(s) 1629
Psp1406I AACGTT 3 cut(s) 430, 1615, 3282
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspFI CCCAGC 4 cut(s) 892, 2804, 3136, 3381
PspGI CCWGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspOMI GGGCCC 1 cut(s) 3677
PspPPI RGGWCCY 1 cut(s) 1017
PstI CTGCAG 1 cut(s) 3649
PstNI CAGNNNCTG 2 cut(s) 1694, 1933
PsuI RGATCY 4 cut(s) 989, 1522, 3377, 3393
PvuII CAGCTG 2 cut(s) 2587, 3385
RseI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SacI GAGCTC 1 cut(s) 1629
SapI GCTCTTC 2 cut(s) 2766, 3889
SaqAI TTAA 7 cut(s) 1077, 2231, 2361, 2946, 3042, 3348, 3516
SchI GAGTC 2 cut(s) 188, 272
ScrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
SduI GDGCHC 5 cut(s) 1629, 2638, 3040, 3562, 3681
SexAI ACCWGGT 1 cut(s) 1006
SfcI CTRYAG 1 cut(s) 3645
SfuI TTCGAA 1 cut(s) 1276
SinI GGWCC 5 cut(s) 1017, 2825, 2980, 3097, 3331
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SmlI CTYRAG 3 cut(s) 2936, 2945, 3789
SmoI CTYRAG 3 cut(s) 2936, 2945, 3789
SpeI ACTAGT 1 cut(s) 877
SphI GCATGC 1 cut(s) 1126
SsiI CCGC 8 cut(s) 712, 748, 2515, 2549, 2666, 2813, 3054, 3668
SspI AATATT 2 cut(s) 2662, 3001
SspMI CTAG 6 cut(s) 459, 878, 2742, 3366, 3389, 3489
SstI GAGCTC 1 cut(s) 1629
StyD4I CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
StyI CCWWGG 6 cut(s) 887, 932, 1654, 1982, 2914, 3067
TaqI TCGA 8 cut(s) 106, 655, 673, 1276, 1357, 1864, 2685, 3313
TaqII GACCGA 1 cut(s) 2051
TatI WGTACW 5 cut(s) 1444, 2106, 2370, 2847, 3627
TauI GCSGC 2 cut(s) 750, 3057
TfiI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
Tru1I TTAA 7 cut(s) 1077, 2231, 2361, 2946, 3042, 3348, 3516
Tru9I TTAA 7 cut(s) 1077, 2231, 2361, 2946, 3042, 3348, 3516
TseFI GTSAC 4 cut(s) 518, 2272, 2450, 2940
Tsp45I GTSAC 4 cut(s) 518, 2272, 2450, 2940
TspGWI ACGGA 2 cut(s) 395, 2867
Vha464I CTTAAG 1 cut(s) 2945
VpaK11BI GGWCC 5 cut(s) 1017, 2825, 2980, 3097, 3331
XapI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
XceI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
XcmI CCANNNNNNNNNTGG 1 cut(s) 1534
XmiI GTMKAC 2 cut(s) 1562, 2733
XmnI GAANNNNTTC 3 cut(s) 432, 2612, 2958
XspI CTAG 6 cut(s) 459, 878, 2742, 3366, 3389, 3489
ZraI GACGTC 1 cut(s) 2673
Zsp2I ATGCAT 1 cut(s) 2863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.