FvH4_3g13400

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002001
Physical Location & Seq
Forward (+)
8003823 .. 8007391
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13400.t1

Sequence Viewer

Length: 3192 bp
ATGGTGGAATCGAAACTCGAGAAGAGCGGAGGAGGAGGAGGCGGCGGCGGAGGATTGCATGTTCGCGGAACGGAGAGAGTTGTTTTCAGGCATCCGGCGAAGAAATTAGGGTTTGATGGAGGTGTCAGGAGTAGGTATGATGAAAGAAGAGATTACAGAGATGGTGGTGGTGGTGGTAGGAGTAGGGTGAGGCGAAGAGATGAAGAAGATGGATATGGTGGAGATTGTGGAAGAAAGAGAAGTAGATATGATGGTGGTTCGAAGAGGGAGCCGCATTCGGTTGTGTCTGCCTGGGATTGTATGTCTTACTCTCCCCTGGTGCAGATACGTGCTTCTGGAGAGGGAGTGAAATCTTTGGATTCGAGACGTGGTGGGAGATATGAGATTAGTGAGAGCATGCGTTTGGAGATGGAGTATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCGCGGTGGTTGATACGAGTGATGGTTCCGGTTTGTTTTATGGGGATGATGCTTTTTGTCAGAAGAAGGAAGCGGAGCTGGCGAAGAGGCTGGTTAGGAGAGATGGGACTAAGATGAGCCGTGCTCAGAGCAAGAGGTTGGCGCAGCATGCTGCAGATAATGCTCAGTGGGAGGACCGTCAGCTTCTGCGGTCGGGTGCTGTTAGAGGTGTTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAGCAAAGTTATGCTCCTTGTTCATGATACAAAGCCTCCTTTCCTGGATGGGAGAGTTGTTTTTACCAATCAGGCTGATCCGGTTTTGCCGGTGAAAGATCCCACATCTGATATGGCCGTAATTGCACGCAAAGGGTCTGCTCTAGTTAGGGGAATCCATCAAAAGCAAACTTTGAATAAATCACGTCAGCGATTTTGGGAGCTTGCTGGTTCGAAACTTGGTGATATTCTTGGTGTTGAGAAAACAGCTGAGCATATTGATGCGGATACGTCTTTAGTAGGTAAAGACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCTCAGCATATGAAGAATGGGGATGCTGTGAGTGAATTCACAAAGTCGAAAACCATCTCCGAACAACGCCAGTATCTGCCTATATATTCTGTGCGTGATGAATTACTGCAGCGAATTCGAGAAAATCAGGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGCTACACTGTAAACGGTATAGTGGGCTGCACCCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCGAAGAGAGTTAGTGAAGAGATGGAAACTGACCTTGGTGACAAAGTTGGCTATGCTATTCGTTTTGAGGACGTGACAGGGGCAAACACGGTTATAAAGTACATGACTGATGGAGTACTTATGCGTGAAACACTCAGAGATTCTGACCTTGACAAGTACCGTGTAATTGTGATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATTTTGAAAAAAGTTGTTGCCCAACGTCGTGATTTTAAGCTCATTGTGACATCTGCTACTCTAAATGCTCAGAAATTTTCAGATTTCTTTGGAGGTGCACTGATTTATCCCATTCCTGGAAGAACATTTCCTGTGGATAAAAGGTACATAGGCCCATGTGAAGACTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTGCCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAAATTGAAGCAGCCTGTTATTCTCTTGCAGAGCGAATGGAACAACTTATCTCCTCCTCTAAGAAAGGGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTGAAGATGGGGTTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATCTTGTATGTCGTTGACACCGGCTATGGTAAAATGAAAGTATACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGCGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGTTGTACACAGAGAATGCCTACCTAAATGAAATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTATTGTTGCTGAAATCTCTCAAGGTCAATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCAGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGGCTTGGGCTGGAAAATGGTGGAGTTCCCGTTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTAGGATGCGTAGATGAAATTTTGACAATTGTATCTATGCTTTCAGTTCCGTCAGTATTCTTTAGGCCAAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCGGAGTCTGACCACTTGACATTATATAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAATGACCATTTTTTGCATGTTAAAGGGTTACAAAAGGCTAGAGAGGTTAGGTCACAGCTGCTAGAGATTCTGAAGACGTTGAAAGTTTCACTGACATCTTGTTGGCCTGATACTGACACTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCAGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATAGGCTGCACCCCTGAGTATGTGGTTTATCATGAACTGATTTTGACAAAAAAGGAGTATATGCAGTGTGTCACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGGCATCAGGAAGAGAAAACAGCTATGGAGGAGGAAATGGAGAATCTGAGGAAGGCTCAAGCACGGGAAGAGGCAGTAAGCAAGCAGAGGGAGAGGGAAAAGAGGTCTAAACAGCAGCAGCAAGTCTCATTGGTGGGTTTGCATCCGGGTTCTTCTACGTATTTGAGGCCGAAGAAACTTGGCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1064

Amino Acids

119.29

Weight (kDa)

6.88

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 373 - 525 8.1e-09 DEAD/DEAH box helicase
AAA_22 PF13401 381 - 514 3.4e-06 AAA domain
Helicase_C PF00271 570 - 699 1.1e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 764 - 789 5.1e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 790 - 849 9.6e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 907 - 983 1e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1376, 3190
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 2004
AccII CGCG 3 cut(s) 66, 458, 2073
AclWI GGATC 3 cut(s) 770, 791, 2164
AcoI YGGCCR 3 cut(s) 813, 1744, 2151
AcsI RAATTY 6 cut(s) 1055, 1134, 1574, 1698, 2424, 2513
AcuI CTGAAG 2 cut(s) 1917, 2690
AfaI GTAC 7 cut(s) 1382, 1398, 1439, 1646, 2120, 2163, 2288
AfiI CCNNNNNNNGG 6 cut(s) 1257, 1470, 1616, 2017, 2018, 2296
AflIII ACRYGT 1 cut(s) 2105
AgsI TTSAA 8 cut(s) 874, 1003, 1507, 1673, 1760, 2036, 2680, 2750
AjiI CACGTC 3 cut(s) 368, 884, 1354
AjnI CCWGG 7 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262
AjuI GAANNNNNNNTTGG 2 cut(s) 1299, 1331
AleI CACNNNNGTG 1 cut(s) 2918
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
Alw21I GWGCWC 4 cut(s) 580, 1600, 2311, 2833
Alw26I GTCTC 3 cut(s) 358, 1934, 3133
Alw44I GTGCAC 1 cut(s) 1596
AlwI GGATC 3 cut(s) 770, 791, 2164
AlwNI CAGNNNCTG 2 cut(s) 640, 1096
Ama87I CYCGRG 3 cut(s) 17, 2505, 2821
AoxI GGCC 9 cut(s) 813, 1651, 1689, 1744, 2096, 2151, 2471, 2702, 3170
ApaLI GTGCAC 1 cut(s) 1596
ApoI RAATTY 6 cut(s) 1055, 1134, 1574, 1698, 2424, 2513
Asp700I GAANNNNTTC 1 cut(s) 2271
AspA2I CCTAGG 1 cut(s) 2012
AspLEI GCGC 3 cut(s) 458, 598, 2075
AspS9I GGNCC 6 cut(s) 628, 1652, 2096, 2251, 2368, 2948
AsuC2I CCSGG 2 cut(s) 2525, 3150
AsuHPI GGTGA 7 cut(s) 199, 802, 932, 1001, 1331, 1733, 2411
AsuII TTCGAA 2 cut(s) 260, 911
AvaI CYCGRG 3 cut(s) 17, 2505, 2821
AvaII GGWCC 4 cut(s) 628, 2251, 2368, 2948
AvrII CCTAGG 1 cut(s) 2012
BaeGI GKGCMC 1 cut(s) 1600
BaeI ACNNNNGTAYC 8 cut(s) 1076, 1109, 2421, 2454, 2564, 2597, 2970, 3003
BalI TGGCCA 1 cut(s) 2153
BanII GRGCYC 1 cut(s) 2311
BbsI GAAGAC 2 cut(s) 1668, 2678
Bbv12I GWGCWC 4 cut(s) 580, 1600, 2311, 2833
BceAI ACGGC 2 cut(s) 558, 800
BcgI CGANNNNNNTGC 2 cut(s) 1118, 1152
BciT130I CCWGG 7 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264
BciVI GTATCC 2 cut(s) 958, 2971
BcnI CCSGG 2 cut(s) 2525, 3150
BcoDI GTCTC 3 cut(s) 358, 1934, 3133
BfaI CTAG 6 cut(s) 842, 2013, 2046, 2408, 2637, 2660
BfmI CTRYAG 3 cut(s) 606, 1127, 1677
BfuI GTATCC 2 cut(s) 958, 2971
BlnI CCTAGG 1 cut(s) 2012
BlpI GCTNAGC 2 cut(s) 948, 1023
BmcAI AGTACT 1 cut(s) 1398
Bme1390I CCNGG 9 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264, 2525, 3150
Bme18I GGWCC 4 cut(s) 628, 2251, 2368, 2948
BmeT110I CYCGRG 3 cut(s) 17, 2505, 2821
BmgBI CACGTC 3 cut(s) 368, 884, 1354
BmgT120I GGNCC 6 cut(s) 628, 1652, 2096, 2251, 2368, 2948
BmiI GGNNCC 7 cut(s) 270, 481, 2253, 2370, 2925, 2949, 2950
BmrFI CCNGG 9 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264, 2525, 3150
BmrI ACTGGG 2 cut(s) 2103, 2808
BmuI ACTGGG 2 cut(s) 2103, 2808
BpiI GAAGAC 2 cut(s) 1668, 2678
BplI GAGNNNNNCTC 6 cut(s) 562, 594, 2815, 2847, 3043, 3075
BpmI CTGGAG 2 cut(s) 357, 1701
Bpu1102I GCTNAGC 2 cut(s) 948, 1023
Bpu14I TTCGAA 2 cut(s) 260, 911
BpuEI CTTGAG 2 cut(s) 2201, 3045
BpuMI CCSGG 2 cut(s) 2525, 3150
BsaAI YACGTR 3 cut(s) 329, 2194, 3162
BsaJI CCNNGG 7 cut(s) 291, 315, 1256, 1315, 2012, 2185, 2262
BsaWI WCCGGW 2 cut(s) 482, 778
Bsc4I CCNNNNNNNGG 6 cut(s) 1257, 1470, 1616, 2017, 2018, 2296
Bse118I RCCGGY 3 cut(s) 787, 1742, 1982
Bse1I ACTGG 7 cut(s) 1090, 1177, 2098, 2154, 2338, 2597, 2803
Bse3DI GCAATG 2 cut(s) 1704, 2605
BseBI CCWGG 7 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264
BseDI CCNNGG 7 cut(s) 291, 315, 1256, 1315, 2012, 2185, 2262
BseLI CCNNNNNNNGG 6 cut(s) 1257, 1470, 1616, 2017, 2018, 2296
BseMI GCAATG 2 cut(s) 1704, 2605
BseMII CTCAG 9 cut(s) 593, 632, 939, 1037, 1429, 1583, 1929, 2847, 3041
BseNI ACTGG 7 cut(s) 1090, 1177, 2098, 2154, 2338, 2597, 2803
BseRI GAGGAG 7 cut(s) 45, 48, 51, 1795, 1798, 2504, 3047
BseSI GKGCMC 1 cut(s) 1600
BseYI CCCAGC 1 cut(s) 2075
BsgI GTGCAG 4 cut(s) 341, 692, 1231, 2833
Bsh1236I CGCG 3 cut(s) 66, 458, 2073
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 9 cut(s) 815, 1653, 1691, 1746, 2098, 2153, 2473, 2704, 3172
BsiEI CGRYCG 1 cut(s) 647
BsiHKAI GWGCWC 4 cut(s) 580, 1600, 2311, 2833
BsiHKCI CYCGRG 3 cut(s) 17, 2505, 2821
BsiSI CCGG 9 cut(s) 95, 483, 779, 788, 1743, 1983, 2525, 2794, 3149
BslFI GGGAC 4 cut(s) 574, 2507, 2603, 2961
BslI CCNNNNNNNGG 6 cut(s) 1257, 1470, 1616, 2017, 2018, 2296
BsmAI GTCTC 3 cut(s) 358, 1934, 3133
BsmBI CGTCTC 1 cut(s) 358
BsmFI GGGAC 4 cut(s) 574, 2507, 2603, 2961
BsmI GAATGC 2 cut(s) 274, 2134
BsnI GGCC 9 cut(s) 815, 1653, 1691, 1746, 2098, 2153, 2473, 2704, 3172
BsoBI CYCGRG 3 cut(s) 17, 2505, 2821
Bsp119I TTCGAA 2 cut(s) 260, 911
Bsp1286I GDGCHC 4 cut(s) 580, 1600, 2311, 2833
Bsp1407I TGTACA 1 cut(s) 2118
Bsp143I GATC 3 cut(s) 775, 796, 2169
Bsp1720I GCTNAGC 2 cut(s) 948, 1023
BspANI GGCC 9 cut(s) 815, 1653, 1691, 1746, 2098, 2153, 2473, 2704, 3172
BspCNI CTCAG 9 cut(s) 592, 631, 940, 1036, 1428, 1582, 1930, 2848, 3042
BspFNI CGCG 3 cut(s) 66, 458, 2073
BspHI TCATGA 3 cut(s) 721, 1737, 2872
BspLI GGNNCC 7 cut(s) 270, 481, 2253, 2370, 2925, 2949, 2950
BspMAI CTGCAG 3 cut(s) 610, 1131, 1681
BspPI GGATC 3 cut(s) 770, 791, 2164
BspQI GCTCTTC 3 cut(s) 17, 2037, 2397
BspT104I TTCGAA 2 cut(s) 260, 911
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 2 cut(s) 1704, 2605
BsrFI RCCGGY 3 cut(s) 787, 1742, 1982
BsrGI TGTACA 1 cut(s) 2118
BsrI ACTGG 7 cut(s) 1090, 1177, 2098, 2154, 2338, 2597, 2803
BssAI RCCGGY 3 cut(s) 787, 1742, 1982
BssECI CCNNGG 7 cut(s) 291, 315, 1256, 1315, 2012, 2185, 2262
BssMI GATC 3 cut(s) 775, 796, 2169
BssNAI GTATAC 1 cut(s) 2005
BssT1I CCWWGG 4 cut(s) 1256, 1315, 2012, 2185
Bst1107I GTATAC 1 cut(s) 2005
Bst2UI CCWGG 7 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264
BstAPI GCANNNNNTGC 2 cut(s) 614, 1019
BstAUI TGTACA 1 cut(s) 2118
BstBAI YACGTR 3 cut(s) 329, 2194, 3162
BstBI TTCGAA 2 cut(s) 260, 911
BstFNI CGCG 3 cut(s) 66, 458, 2073
BstHHI GCGC 3 cut(s) 458, 598, 2075
BstKTI GATC 3 cut(s) 778, 799, 2172
BstMAI GTCTC 3 cut(s) 358, 1934, 3133
BstMBI GATC 3 cut(s) 775, 796, 2169
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 6 cut(s) 533, 602, 614, 821, 1019, 2081
BstNI CCWGG 7 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264
BstNSI RCATGY 5 cut(s) 62, 400, 605, 2109, 2618
BstSCI CCNGG 9 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262, 2523, 3148
BstSFI CTRYAG 3 cut(s) 606, 1127, 1677
BstSLI GKGCMC 1 cut(s) 1600
BstSNI TACGTA 1 cut(s) 3162
BstUI CGCG 3 cut(s) 66, 458, 2073
BstV2I GAAGAC 2 cut(s) 1668, 2678
BstX2I RGATCY 2 cut(s) 796, 2169
BstXI CCANNNNNNTGG 1 cut(s) 2075
BstYI RGATCY 2 cut(s) 796, 2169
BstZ17I GTATAC 1 cut(s) 2005
BsuI GTATCC 2 cut(s) 958, 2971
BsuRI GGCC 9 cut(s) 815, 1653, 1691, 1746, 2098, 2153, 2473, 2704, 3172
BtgZI GCGATG 1 cut(s) 2511
BtrI CACGTC 3 cut(s) 368, 884, 1354
BtsI GCAGTG 5 cut(s) 1686, 2833, 2912, 2925, 2936
CaiI CAGNNNCTG 2 cut(s) 640, 1096
CciI TCATGA 3 cut(s) 721, 1737, 2872
CfoI GCGC 3 cut(s) 458, 598, 2075
Cfr10I RCCGGY 3 cut(s) 787, 1742, 1982
Cfr13I GGNCC 6 cut(s) 628, 1652, 2096, 2251, 2368, 2948
Csp6I GTAC 7 cut(s) 1381, 1397, 1438, 1645, 2119, 2162, 2287
CspCI CAANNNNNGTGG 2 cut(s) 1245, 1280
CviQI GTAC 7 cut(s) 1381, 1397, 1438, 1645, 2119, 2162, 2287
DpnI GATC 3 cut(s) 777, 798, 2171
DpnII GATC 3 cut(s) 775, 796, 2169
EaeI YGGCCR 3 cut(s) 813, 1744, 2151
EciI GGCGGA 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 2309
Eco105I TACGTA 1 cut(s) 3162
Eco130I CCWWGG 4 cut(s) 1256, 1315, 2012, 2185
Eco24I GRGCYC 1 cut(s) 2311
Eco32I GATATC 1 cut(s) 1726
Eco47I GGWCC 4 cut(s) 628, 2251, 2368, 2948
Eco53kI GAGCTC 1 cut(s) 2309
Eco57I CTGAAG 2 cut(s) 1917, 2690
Eco88I CYCGRG 3 cut(s) 17, 2505, 2821
EcoICRI GAGCTC 1 cut(s) 2309
EcoO109I RGGNCCY 1 cut(s) 2948
EcoRI GAATTC 2 cut(s) 1055, 1134
EcoRII CCWGG 7 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262
EcoRV GATATC 1 cut(s) 1726
EcoT14I CCWWGG 4 cut(s) 1256, 1315, 2012, 2185
EcoT22I ATGCAT 1 cut(s) 1919
EcoT38I GRGCYC 1 cut(s) 2311
ErhI CCWWGG 4 cut(s) 1256, 1315, 2012, 2185
Esp3I CGTCTC 1 cut(s) 358
FaqI GGGAC 4 cut(s) 574, 2507, 2603, 2961
FauNDI CATATG 1 cut(s) 1029
FblI GTMKAC 1 cut(s) 2004
FriOI GRGCYC 1 cut(s) 2311
FspBI CTAG 6 cut(s) 842, 2013, 2046, 2408, 2637, 2660
GlaI GCGC 3 cut(s) 457, 597, 2074
GsaI CCCAGC 1 cut(s) 2079
GsuI CTGGAG 2 cut(s) 357, 1701
HaeIII GGCC 9 cut(s) 815, 1653, 1691, 1746, 2098, 2153, 2473, 2704, 3172
HapII CCGG 9 cut(s) 95, 483, 779, 788, 1743, 1983, 2525, 2794, 3149
HhaI GCGC 3 cut(s) 458, 598, 2075
Hin6I GCGC 3 cut(s) 456, 596, 2073
HinP1I GCGC 3 cut(s) 456, 596, 2073
HincII GTYRAC 1 cut(s) 1978
HindII GTYRAC 1 cut(s) 1978
HinfI GANTC 8 cut(s) 8, 359, 852, 1421, 2528, 2665, 2972, 3046
HpaII CCGG 9 cut(s) 95, 483, 779, 788, 1743, 1983, 2525, 2794, 3149
HphI GGTGA 7 cut(s) 199, 802, 932, 1001, 1331, 1733, 2411
Hpy166II GTNNAC 5 cut(s) 1231, 1598, 1978, 2005, 2121
Hpy8I GTNNAC 5 cut(s) 1231, 1598, 1978, 2005, 2121
Hpy99I CGWCG 1 cut(s) 1530
HpyAV CCTTC 3 cut(s) 443, 514, 3049
HpyCH4IV ACGT 9 cut(s) 328, 367, 883, 968, 1353, 1525, 2193, 2675, 3161
HpyF10VI GCNNNNNNNGC 6 cut(s) 533, 602, 614, 821, 1019, 2081
HpySE526I ACGT 9 cut(s) 328, 367, 883, 968, 1353, 1525, 2193, 2675, 3161
HspAI GCGC 3 cut(s) 456, 596, 2073
KflI GGGWCCC 1 cut(s) 2948
Kzo9I GATC 3 cut(s) 775, 796, 2169
LguI GCTCTTC 3 cut(s) 17, 2037, 2397
LmnI GCTCC 6 cut(s) 268, 529, 717, 898, 2306, 2923
MaeI CTAG 6 cut(s) 842, 2013, 2046, 2408, 2637, 2660
MaeII ACGT 9 cut(s) 328, 367, 883, 968, 1353, 1525, 2193, 2675, 3161
MaeIII GTNAC 9 cut(s) 422, 1319, 1354, 1473, 1546, 1681, 2625, 2649, 2911
MalI GATC 3 cut(s) 777, 798, 2171
MbiI CCGCTC 1 cut(s) 27
MboI GATC 3 cut(s) 775, 796, 2169
MfeI CAATTG 1 cut(s) 2433
MflI RGATCY 2 cut(s) 796, 2169
MhlI GDGCHC 4 cut(s) 580, 1600, 2311, 2833
MlsI TGGCCA 1 cut(s) 2153
MluNI TGGCCA 1 cut(s) 2153
MlyI GAGTC 2 cut(s) 2537, 2981
MmeI TCCRAC 3 cut(s) 1144, 2346, 2955
Mox20I TGGCCA 1 cut(s) 2153
Mph1103I ATGCAT 1 cut(s) 1919
MroXI GAANNNNTTC 1 cut(s) 2271
MscI TGGCCA 1 cut(s) 2153
MseI TTAA 4 cut(s) 1536, 2619, 2766, 2787
MslI CAYNNNNRTG 4 cut(s) 957, 1215, 1736, 2918
Msp20I TGGCCA 1 cut(s) 2153
MspA1I CMGCKG 5 cut(s) 947, 1858, 1865, 2293, 2656
MspI CCGG 9 cut(s) 95, 483, 779, 788, 1743, 1983, 2525, 2794, 3149
MspR9I CCNGG 9 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264, 2525, 3150
MunI CAATTG 1 cut(s) 2433
Mva1269I GAATGC 2 cut(s) 274, 2134
MvaI CCWGG 7 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264
MvnI CGCG 3 cut(s) 66, 458, 2073
MwoI GCNNNNNNNGC 6 cut(s) 533, 602, 614, 821, 1019, 2081
NciI CCSGG 2 cut(s) 2525, 3150
NdeI CATATG 1 cut(s) 1029
NdeII GATC 3 cut(s) 775, 796, 2169
NlaIV GGNNCC 7 cut(s) 270, 481, 2253, 2370, 2925, 2949, 2950
NmuCI GTSAC 8 cut(s) 422, 1319, 1354, 1473, 1546, 1681, 2649, 2911
NsiI ATGCAT 1 cut(s) 1919
NspI RCATGY 5 cut(s) 62, 400, 605, 2109, 2618
NspV TTCGAA 2 cut(s) 260, 911
OliI CACNNNNGTG 1 cut(s) 2918
PaeI GCATGC 2 cut(s) 400, 605
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 3 cut(s) 721, 1737, 2872
PciI ACATGT 1 cut(s) 2105
PciSI GCTCTTC 3 cut(s) 17, 2037, 2397
PcsI WCGNNNNNNNCGW 1 cut(s) 2077
PctI GAATGC 2 cut(s) 274, 2134
PdmI GAANNNNTTC 1 cut(s) 2271
PfeI GAWTC 6 cut(s) 8, 359, 852, 1421, 2665, 3046
PfoI TCCNGGA 3 cut(s) 741, 1615, 2523
PleI GAGTC 2 cut(s) 2536, 2980
PpsI GAGTC 2 cut(s) 2536, 2980
Ppu21I YACGTR 3 cut(s) 329, 2194, 3162
PpuMI RGGWCCY 1 cut(s) 2948
PscI ACATGT 1 cut(s) 2105
PsiI TTATAA 2 cut(s) 1376, 3190
Psp124BI GAGCTC 1 cut(s) 2311
Psp5II RGGWCCY 1 cut(s) 2948
Psp6I CCWGG 7 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262
PspFI CCCAGC 1 cut(s) 2075
PspGI CCWGG 7 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262
PspN4I GGNNCC 7 cut(s) 270, 481, 2253, 2370, 2925, 2949, 2950
PspPI GGNCC 6 cut(s) 628, 1652, 2096, 2251, 2368, 2948
PspPPI RGGWCCY 1 cut(s) 2948
PsrI GAACNNNNNNTAC 2 cut(s) 2174, 2206
PstI CTGCAG 3 cut(s) 610, 1131, 1681
PstNI CAGNNNCTG 2 cut(s) 640, 1096
PsuI RGATCY 2 cut(s) 796, 2169
PvuII CAGCTG 5 cut(s) 947, 1858, 1865, 2293, 2656
RsaI GTAC 7 cut(s) 1382, 1398, 1439, 1646, 2120, 2163, 2288
RsaNI GTAC 7 cut(s) 1381, 1397, 1438, 1645, 2119, 2162, 2287
RseI CAYNNNNRTG 4 cut(s) 957, 1215, 1736, 2918
SacI GAGCTC 1 cut(s) 2311
SapI GCTCTTC 3 cut(s) 17, 2037, 2397
SaqAI TTAA 4 cut(s) 1536, 2619, 2766, 2787
Sau3AI GATC 3 cut(s) 775, 796, 2169
Sau96I GGNCC 6 cut(s) 628, 1652, 2096, 2251, 2368, 2948
ScaI AGTACT 1 cut(s) 1398
SchI GAGTC 2 cut(s) 2537, 2981
ScrFI CCNGG 9 cut(s) 292, 317, 743, 1617, 1719, 2100, 2264, 2525, 3150
SduI GDGCHC 4 cut(s) 580, 1600, 2311, 2833
SfcI CTRYAG 3 cut(s) 606, 1127, 1677
Sfr274I CTCGAG 1 cut(s) 17
SfuI TTCGAA 2 cut(s) 260, 911
SinI GGWCC 4 cut(s) 628, 2251, 2368, 2948
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 4 cut(s) 957, 1215, 1736, 2918
SmlI CTYRAG 3 cut(s) 17, 2216, 3060
SmoI CTYRAG 3 cut(s) 17, 2216, 3060
SnaBI TACGTA 1 cut(s) 3162
SphI GCATGC 2 cut(s) 400, 605
SspI AATATT 2 cut(s) 1933, 2272
SspMI CTAG 6 cut(s) 842, 2013, 2046, 2408, 2637, 2660
SstI GAGCTC 1 cut(s) 2311
StyD4I CCNGG 9 cut(s) 290, 315, 741, 1615, 1717, 2098, 2262, 2523, 3148
StyI CCWWGG 4 cut(s) 1256, 1315, 2012, 2185
TaiI ACGT 9 cut(s) 331, 370, 886, 971, 1356, 1528, 2196, 2678, 3164
TaqI TCGA 8 cut(s) 11, 18, 260, 362, 911, 1067, 1138, 2313
TatI WGTACW 3 cut(s) 1380, 1396, 2118
TauI GCSGC 4 cut(s) 45, 48, 274, 2929
TfiI GAWTC 6 cut(s) 8, 359, 852, 1421, 2665, 3046
Tru1I TTAA 4 cut(s) 1536, 2619, 2766, 2787
Tru9I TTAA 4 cut(s) 1536, 2619, 2766, 2787
TseFI GTSAC 8 cut(s) 422, 1319, 1354, 1473, 1546, 1681, 2649, 2911
Tsp45I GTSAC 8 cut(s) 422, 1319, 1354, 1473, 1546, 1681, 2649, 2911
TspGWI ACGGA 2 cut(s) 86, 2445
VneI GTGCAC 1 cut(s) 1596
VpaK11BI GGWCC 4 cut(s) 628, 2251, 2368, 2948
XapI RAATTY 6 cut(s) 1055, 1134, 1574, 1698, 2424, 2513
XceI RCATGY 5 cut(s) 62, 400, 605, 2109, 2618
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 17
XmaJI CCTAGG 1 cut(s) 2012
XmiI GTMKAC 1 cut(s) 2004
XmnI GAANNNNTTC 1 cut(s) 2271
XspI CTAG 6 cut(s) 842, 2013, 2046, 2408, 2637, 2660
ZrmI AGTACT 1 cut(s) 1398
Zsp2I ATGCAT 1 cut(s) 1919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.