Rh5AG159400

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002001
Physical Location & Seq
Forward (+)
16939162 .. 16940670
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG159400.1

Sequence Viewer

Length: 1509 bp
ATGAAAATTCACATTGGCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTTGCAGAGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGGGATGTCAAAACTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCAACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTTTACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGCTGTACACAGAGAGTGCCTACCTAAATGAAATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTCAAGTCTCTGAAGGTTGATAACTTGCTTGATTTTGATTTCATGGACCCACCTCCCCTGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAAAATGGTGGAGTTCCCATTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTAGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCAGTCCCAGAGTCCGATCACCTGACATTCTACAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTACAAAAGGCTAGAGAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACGTTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATGGGATGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACAGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGACAATCTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAGCAAGCAGAGGGAGAGGGAAAAGAGGTATAAACAGCAGCAGCAGCAGCAGCAAGTCTCATTGGCGGGTTTGCACCAGGGCTCTTCTACATATCTGAGGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

502

Amino Acids

56.84

Weight (kDa)

7.09

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 8 - 135 7.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 200 - 225 2.6e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 226 - 285 7.5e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 343 - 419 1.9e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 1235, 1454
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 2 cut(s) 52, 459
AcsI RAATTY 4 cut(s) 6, 732, 821, 990
AcuI CTGAAG 2 cut(s) 545, 998
AfaI GTAC 4 cut(s) 428, 471, 596, 1208
AfiI CCNNNNNNNGG 5 cut(s) 325, 326, 604, 651, 789
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 4 cut(s) 68, 344, 988, 1058
AjnI CCWGG 4 cut(s) 25, 406, 570, 1464
AluBI AGCT 8 cut(s) 166, 173, 601, 617, 715, 964, 1037, 1334
AluI AGCT 8 cut(s) 166, 173, 601, 617, 715, 964, 1037, 1334
Alw21I GWGCWC 2 cut(s) 619, 1141
Alw26I GTCTC 4 cut(s) 242, 525, 1306, 1450
AlwI GGATC 1 cut(s) 472
AlwNI CAGNNNCTG 1 cut(s) 1192
Ama87I CYCGRG 2 cut(s) 813, 1129
AoxI GGCC 6 cut(s) 52, 404, 459, 779, 1010, 1487
ApoI RAATTY 4 cut(s) 6, 732, 821, 990
Asp700I GAANNNNTTC 2 cut(s) 579, 1188
AspA2I CCTAGG 1 cut(s) 320
AspS9I GGNCC 7 cut(s) 404, 559, 676, 780, 957, 1256, 1488
AsuHPI GGTGA 3 cut(s) 41, 719, 836
AvaI CYCGRG 2 cut(s) 813, 1129
AvaII GGWCC 4 cut(s) 559, 676, 957, 1256
AvrII CCTAGG 1 cut(s) 320
AxyI CCTNAGG 1 cut(s) 646
BaeI ACNNNNGTAYC 8 cut(s) 729, 762, 872, 905, 1008, 1041, 1278, 1311
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 3 cut(s) 217, 619, 1472
BbsI GAAGAC 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 619, 1141
BccI CCATC 5 cut(s) 203, 260, 319, 697, 772
BciT130I CCWGG 4 cut(s) 27, 408, 572, 1466
BciVI GTATCC 2 cut(s) 757, 1279
BcoDI GTCTC 4 cut(s) 242, 525, 1306, 1450
BfaI CTAG 5 cut(s) 321, 354, 716, 945, 968
BfuI GTATCC 2 cut(s) 757, 1279
BlnI CCTAGG 1 cut(s) 320
Bme1390I CCNGG 4 cut(s) 27, 408, 572, 1466
Bme18I GGWCC 4 cut(s) 559, 676, 957, 1256
BmeT110I CYCGRG 2 cut(s) 813, 1129
BmgT120I GGNCC 7 cut(s) 404, 559, 676, 780, 957, 1256, 1488
BmiI GGNNCC 6 cut(s) 561, 678, 959, 1233, 1257, 1258
BmrFI CCNGG 4 cut(s) 27, 408, 572, 1466
BmrI ACTGGG 2 cut(s) 411, 1116
BmsI GCATC 5 cut(s) 325, 710, 796, 1101, 1145
BmuI ACTGGG 2 cut(s) 411, 1116
BpiI GAAGAC 1 cut(s) 986
BplI GAGNNNNNCTC 4 cut(s) 1123, 1155, 1351, 1383
BpmI CTGGAG 1 cut(s) 9
BpuEI CTTGAG 2 cut(s) 500, 1353
BsaAI YACGTR 1 cut(s) 502
BsaJI CCNNGG 4 cut(s) 320, 493, 570, 1465
BsaXI ACNNNNNCTCC 2 cut(s) 1223, 1253
Bsc4I CCNNNNNNNGG 5 cut(s) 325, 326, 604, 651, 789
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 5 cut(s) 295, 406, 462, 905, 1111
Bse21I CCTNAGG 1 cut(s) 646
BseBI CCWGG 4 cut(s) 27, 408, 572, 1466
BseDI CCNNGG 4 cut(s) 320, 493, 570, 1465
BseGI GGATG 6 cut(s) 135, 330, 340, 725, 1116, 1160
BseLI CCNNNNNNNGG 5 cut(s) 325, 326, 604, 651, 789
BseMII CTCAG 4 cut(s) 237, 1155, 1349, 1475
BseNI ACTGG 5 cut(s) 295, 406, 462, 905, 1111
BseRI GAGGAG 5 cut(s) 74, 103, 106, 812, 1355
BseYI CCCAGC 2 cut(s) 383, 960
BsgI GTGCAG 1 cut(s) 925
BshFI GGCC 6 cut(s) 54, 406, 461, 781, 1012, 1489
BsiHKAI GWGCWC 2 cut(s) 619, 1141
BsiHKCI CYCGRG 2 cut(s) 813, 1129
BsiSI CCGG 2 cut(s) 51, 1102
BslFI GGGAC 4 cut(s) 815, 911, 943, 1269
BslI CCNNNNNNNGG 5 cut(s) 325, 326, 604, 651, 789
BsmAI GTCTC 4 cut(s) 242, 525, 1306, 1450
BsmFI GGGAC 4 cut(s) 815, 911, 943, 1269
BsnI GGCC 6 cut(s) 54, 406, 461, 781, 1012, 1489
BsoBI CYCGRG 2 cut(s) 813, 1129
Bsp1286I GDGCHC 4 cut(s) 217, 619, 1141, 1472
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 2 cut(s) 477, 841
BspACI CCGC 2 cut(s) 1235, 1454
BspANI GGCC 6 cut(s) 54, 406, 461, 781, 1012, 1489
BspCNI CTCAG 4 cut(s) 238, 1156, 1350, 1476
BspHI TCATGA 2 cut(s) 45, 1180
BspLI GGNNCC 6 cut(s) 561, 678, 959, 1233, 1257, 1258
BspPI GGATC 1 cut(s) 472
BspQI GCTCTTC 3 cut(s) 345, 705, 1477
BsrFI RCCGGY 1 cut(s) 50
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 5 cut(s) 295, 406, 462, 905, 1111
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 4 cut(s) 320, 493, 570, 1465
BssMI GATC 2 cut(s) 477, 841
BssT1I CCWWGG 2 cut(s) 320, 493
Bst2UI CCWGG 4 cut(s) 27, 408, 572, 1466
Bst4CI ACNGT 2 cut(s) 262, 873
Bst6I CTCTTC 4 cut(s) 345, 705, 1304, 1477
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 502
BstC8I GCNNGC 5 cut(s) 171, 183, 390, 459, 1394
BstDEI CTNAG 6 cut(s) 246, 646, 690, 1164, 1358, 1484
BstF5I GGATG 6 cut(s) 135, 330, 340, 725, 1116, 1160
BstKTI GATC 2 cut(s) 480, 844
BstMAI GTCTC 4 cut(s) 242, 525, 1306, 1450
BstMBI GATC 2 cut(s) 477, 841
BstMWI GCNNNNNNNGC 5 cut(s) 389, 760, 1432, 1435, 1438
BstNI CCWGG 4 cut(s) 27, 408, 572, 1466
BstNSI RCATGY 3 cut(s) 417, 926, 1213
BstSCI CCNGG 4 cut(s) 25, 406, 570, 1464
BstV2I GAAGAC 1 cut(s) 986
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 477
Bsu36I CCTNAGG 1 cut(s) 646
BsuI GTATCC 2 cut(s) 757, 1279
BsuRI GGCC 6 cut(s) 54, 406, 461, 781, 1012, 1489
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 6 cut(s) 135, 330, 340, 725, 1116, 1160
BtsI GCAGTG 5 cut(s) 913, 1141, 1220, 1233, 1244
Cac8I GCNNGC 5 cut(s) 171, 183, 390, 459, 1394
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 2 cut(s) 45, 1180
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 7 cut(s) 404, 559, 676, 780, 957, 1256, 1488
Csp6I GTAC 4 cut(s) 427, 470, 595, 1207
CviAII CATG 9 cut(s) 46, 414, 556, 751, 923, 1116, 1181, 1210, 1261
CviQI GTAC 4 cut(s) 427, 470, 595, 1207
DdeI CTNAG 6 cut(s) 246, 646, 690, 1164, 1358, 1484
DpnI GATC 2 cut(s) 479, 843
DpnII GATC 2 cut(s) 477, 841
EaeI YGGCCR 2 cut(s) 52, 459
Eam1104I CTCTTC 4 cut(s) 345, 705, 1304, 1477
EarI CTCTTC 4 cut(s) 345, 705, 1304, 1477
Ecl136II GAGCTC 1 cut(s) 617
Eco130I CCWWGG 2 cut(s) 320, 493
Eco24I GRGCYC 3 cut(s) 217, 619, 1472
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 4 cut(s) 559, 676, 957, 1256
Eco53kI GAGCTC 1 cut(s) 617
Eco57I CTGAAG 2 cut(s) 545, 998
Eco81I CCTNAGG 1 cut(s) 646
Eco88I CYCGRG 2 cut(s) 813, 1129
EcoICRI GAGCTC 1 cut(s) 617
EcoO109I RGGNCCY 2 cut(s) 957, 1256
EcoRII CCWGG 4 cut(s) 25, 406, 570, 1464
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 320, 493
EcoT22I ATGCAT 1 cut(s) 227
EcoT38I GRGCYC 3 cut(s) 217, 619, 1472
ErhI CCWWGG 2 cut(s) 320, 493
FaeI CATG 9 cut(s) 49, 417, 559, 754, 926, 1119, 1184, 1213, 1264
FaqI GGGAC 4 cut(s) 815, 911, 943, 1269
FatI CATG 9 cut(s) 45, 413, 555, 750, 922, 1115, 1180, 1209, 1260
FauI CCCGC 1 cut(s) 1447
FokI GGATG 6 cut(s) 142, 337, 347, 732, 1123, 1167
FriOI GRGCYC 3 cut(s) 217, 619, 1472
FspBI CTAG 5 cut(s) 321, 354, 716, 945, 968
GsaI CCCAGC 2 cut(s) 387, 964
GsuI CTGGAG 1 cut(s) 9
HaeIII GGCC 6 cut(s) 54, 406, 461, 781, 1012, 1489
HapII CCGG 2 cut(s) 51, 1102
Hin1II CATG 9 cut(s) 49, 417, 559, 754, 926, 1119, 1184, 1213, 1264
HinfI GANTC 4 cut(s) 836, 973, 1189, 1280
HpaII CCGG 2 cut(s) 51, 1102
HphI GGTGA 3 cut(s) 41, 719, 836
Hpy166II GTNNAC 2 cut(s) 313, 429
Hpy188I TCNGA 7 cut(s) 525, 841, 978, 1194, 1285, 1359, 1485
Hpy188III TCNNGA 6 cut(s) 46, 142, 481, 620, 815, 1181
Hpy8I GTNNAC 2 cut(s) 313, 429
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 2 cut(s) 262, 873
HpyCH4IV ACGT 4 cut(s) 379, 501, 983, 1023
HpyF10VI GCNNNNNNNGC 5 cut(s) 389, 760, 1432, 1435, 1438
HpyF3I CTNAG 6 cut(s) 246, 646, 690, 1164, 1358, 1484
HpySE526I ACGT 4 cut(s) 379, 501, 983, 1023
Hsp92II CATG 9 cut(s) 49, 417, 559, 754, 926, 1119, 1184, 1213, 1264
KflI GGGWCCC 1 cut(s) 1256
Kzo9I GATC 2 cut(s) 477, 841
LguI GCTCTTC 3 cut(s) 345, 705, 1477
LmnI GCTCC 2 cut(s) 614, 1231
LweI GCATC 5 cut(s) 325, 710, 796, 1101, 1145
MaeI CTAG 5 cut(s) 321, 354, 716, 945, 968
MaeII ACGT 4 cut(s) 379, 501, 983, 1023
MaeIII GTNAC 3 cut(s) 908, 933, 1219
MalI GATC 2 cut(s) 479, 843
MboI GATC 2 cut(s) 477, 841
MboII GAAGA 7 cut(s) 34, 332, 722, 991, 1321, 1334, 1464
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 4 cut(s) 217, 619, 1141, 1472
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 7 cut(s) 6, 586, 732, 741, 821, 990, 1096
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 2 cut(s) 845, 1289
MmeI TCCRAC 1 cut(s) 1263
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 1 cut(s) 227
MroXI GAANNNNTTC 2 cut(s) 579, 1188
MscI TGGCCA 1 cut(s) 461
MseI TTAA 3 cut(s) 927, 1074, 1095
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 4 cut(s) 166, 173, 601, 964
MspI CCGG 2 cut(s) 51, 1102
MspR9I CCNGG 4 cut(s) 27, 408, 572, 1466
MunI CAATTG 1 cut(s) 741
MvaI CCWGG 4 cut(s) 27, 408, 572, 1466
MwoI GCNNNNNNNGC 5 cut(s) 389, 760, 1432, 1435, 1438
NdeII GATC 2 cut(s) 477, 841
NlaIII CATG 9 cut(s) 49, 417, 559, 754, 926, 1119, 1184, 1213, 1264
NlaIV GGNNCC 6 cut(s) 561, 678, 959, 1233, 1257, 1258
NmuCI GTSAC 1 cut(s) 908
NsiI ATGCAT 1 cut(s) 227
NspI RCATGY 3 cut(s) 417, 926, 1213
PagI TCATGA 2 cut(s) 45, 1180
PciI ACATGT 1 cut(s) 413
PciSI GCTCTTC 3 cut(s) 345, 705, 1477
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PdmI GAANNNNTTC 2 cut(s) 579, 1188
PfeI GAWTC 2 cut(s) 973, 1189
PleI GAGTC 2 cut(s) 844, 1288
PpsI GAGTC 2 cut(s) 844, 1288
Ppu21I YACGTR 1 cut(s) 502
PpuMI RGGWCCY 2 cut(s) 957, 1256
PscI ACATGT 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 619
Psp5II RGGWCCY 2 cut(s) 957, 1256
Psp6I CCWGG 4 cut(s) 25, 406, 570, 1464
PspFI CCCAGC 2 cut(s) 383, 960
PspGI CCWGG 4 cut(s) 25, 406, 570, 1464
PspN4I GGNNCC 6 cut(s) 561, 678, 959, 1233, 1257, 1258
PspPI GGNCC 7 cut(s) 404, 559, 676, 780, 957, 1256, 1488
PspPPI RGGWCCY 2 cut(s) 957, 1256
PsrI GAACNNNNNNTAC 2 cut(s) 482, 514
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 4 cut(s) 166, 173, 601, 964
RsaI GTAC 4 cut(s) 428, 471, 596, 1208
RsaNI GTAC 4 cut(s) 427, 470, 595, 1207
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 619
SapI GCTCTTC 3 cut(s) 345, 705, 1477
SaqAI TTAA 3 cut(s) 927, 1074, 1095
Sau3AI GATC 2 cut(s) 477, 841
Sau96I GGNCC 7 cut(s) 404, 559, 676, 780, 957, 1256, 1488
SchI GAGTC 2 cut(s) 845, 1289
ScrFI CCNGG 4 cut(s) 27, 408, 572, 1466
SduI GDGCHC 4 cut(s) 217, 619, 1141, 1472
SfaNI GCATC 5 cut(s) 325, 710, 796, 1101, 1145
SinI GGWCC 4 cut(s) 559, 676, 957, 1256
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 515, 1368
SmoI CTYRAG 2 cut(s) 515, 1368
Sse9I AATT 7 cut(s) 6, 586, 732, 741, 821, 990, 1096
SsiI CCGC 2 cut(s) 1235, 1454
SspI AATATT 2 cut(s) 241, 580
SspMI CTAG 5 cut(s) 321, 354, 716, 945, 968
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 4 cut(s) 25, 406, 570, 1464
StyI CCWWGG 2 cut(s) 320, 493
TaaI ACNGT 2 cut(s) 262, 873
TaiI ACGT 4 cut(s) 382, 504, 986, 1026
TaqI TCGA 1 cut(s) 621
TasI AATT 7 cut(s) 6, 586, 732, 741, 821, 990, 1096
TatI WGTACW 2 cut(s) 426, 1206
TauI GCSGC 1 cut(s) 1237
TfiI GAWTC 2 cut(s) 973, 1189
Tru1I TTAA 3 cut(s) 927, 1074, 1095
Tru9I TTAA 3 cut(s) 927, 1074, 1095
TseFI GTSAC 1 cut(s) 908
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 7 cut(s) 17, 34, 320, 465, 544, 744, 1197
VpaK11BI GGWCC 4 cut(s) 559, 676, 957, 1256
XapI RAATTY 4 cut(s) 6, 732, 821, 990
XceI RCATGY 3 cut(s) 417, 926, 1213
XmaJI CCTAGG 1 cut(s) 320
XmnI GAANNNNTTC 2 cut(s) 579, 1188
XspI CTAG 5 cut(s) 321, 354, 716, 945, 968
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.