Rmu_sc0006966.1_g000004

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006966.1
OG0002001
Physical Location & Seq
Forward (+)
20039 .. 27297
7259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006966.1_g000004.1.cds

Sequence Viewer

Length: 3996 bp
atggagccaatgagttcatcaaacccagattctaaaagcgttggtgcggctgaagtggagggttttcgggattctgtcaatgaaatttcagcaaaagttaatacgcttgagaaaagagtgaatgaggtcgagcactattacttgaggaaaggcagtctgcaaccaaccacttcgagggagaaagacagggataaacactttaacaccattaagaagcatcaacaagatgcagcacgtagggaagccgctgctgcaaagagaatgcgagatctgttgaataagttttccgcaatctttaaacagatcactcagcacaagtggtcatggccctttatgtcgcccgttgatgttgaaagtctgggattgcatgactattatcaagttattgagaagcccatggaccttggtacagtacaaagtaaaatggaaaccggtgggtataagagtgttagagagatatatgctgatgtgaggttggtattcaagaatgcaatcaaatacaatgatgacaaagatgatgtccacgtgatggcccaaacactgttggaaaaattagaagaaaagtggttacaactcttgcctaaagttgttgaagaggagaaaagacaagcagatgaggaagcacaggcacaggtagacatgaaacacgctgaggaggttgcttatgccagcatggccaaggatctaagcagcgagctttttgagattgataagtatttgaaagatctcaaaaaagtggtggttcagaagtgcagaaaaatgtcttttgatgataaaagacgacttgggacagctcttacacagttatctcctgaagatctcagcaaggctttggatatagttgctcaggataatcctgagttccaagcaactgcgcaagaggtggatcttgacattgatactcagagcgactacacattatggaggttaaaggtttttgtcaaggatgcattaaaagttaaggcccaagctgctgcacaaattcctgaagccctgggtggcaacaaagttgcaacctccaaaagaaatagggagccctctgatgctgttgccaagcctgccatacgggcttctggacagtcagtcaagtcttcgggctcgggacgtggtgggagttctcatcagcttgatagtacgctaggagaagaggagggtgattcggttgaagggagatatgagattggtgagagcatgcgtttagagatggaatatgattgtgaccgtgcgtggtatgatagagaagaaggaagcacagcggttgatacgagtgatagttcgtctttgttttatggggatgatgctttttgtcgaaagaaggaaacggagttggcgaagagactggctcggagagatggaactaagatgagccttgctcagagcaaaaggttgtctcagcgcagtgcagataatgctcagtgggaggatcgtcagcttctgcgatctggtgctgttagaggtactgaggtgcagagtgagattgatgatgaagatgagaacaaagttatacttcttgttcatgatacaaggcctcctttcctggatgggagagttgtttttaccaagcaagcggagccggttatgccagtaaaggatcccacgtctgatatggccataatttcacgcaaagggtctgctctagttagggaaatccatcaaaagcaaagttcgaataaatcacgtcaacgtttttgggagctttcaggttccaaacttggtgatattcttggtgttgagaaaacagcagagcatattgatgcagatactgctgtagtaggtaaagacggtgaagttgatttcaaagaagatgcaaagtttgctcagcatatgaagaatggggaggctgtgagtgaattcacaaagtcaaaaaccatttccgaacaacgccagtatctgcctatatattctgtgcgggatgagttactgcagaaaattcgagaaaatcaagttattatagttgttggagaaactggttctggaaagacaactcaactgacacaatatctgcatgaagatggttacactgtgaacggtatagtcggttgcacccagccaagacgtgtggcagccatgagtgttgctaaaagagttagtgaagagatggagactgagcttggtgagaaagtgggctatgctattcgttttgaggatgtgacggggccaaaaactgtaataaagtacatgactgatggagtacttatgcgtgaaacacttagagattctgaccttgacaagtaccgtgtagttgtaatggatgaagcccatgagaggtcactatatacagatgtgctgtttgggattttgaaaaaagtcgtcgcccagcgtcgtgattttaagctcattgtgacatctgctactcttaatgctcagaaatttgcagatttctttggaggtgcacctatttttcccattcctggaagaacatttcctgtacataagaagtacttggataccccatgtgaagattatgttgaagctgcagtgacacaggccatgaaaattcacattggcagccctccgggtgatatcctcatcttcatgaccggccaagaggagattgaagcagcctgttattctcttgcagagcgaatggaacaacttatctcctcctcaaagaaagggatgtcaaaactcttgatactccctatgtattcacagctgccagctgacttgcaagcaaagatattccaaaggtctaaagatggggctcgtaaatgcattatagcaacaaatattgctgagacatctttgaccgtagatggtatattttatgtcattgacactggctatggtaaaatgaaagtttacaaccctaggatgggtatggatgctcttcaagtatttcctagtagtcgtgctgctgctgaccaacgtgctgggcgtgctggtagaactgggcctggaacatgttatcggctgtacacagagagtgcctacctaaatgaaatgctggccagtcctgtaccagagatccagaggacgaaccttggctacgtggtcttgttgctcaagtctctgaaggttgataacttgcttgattttgatttcatggacccacctcccctggaaaatattctcaattctatgtaccagctgtgggtcttgggagctctcgacaatgtgggaggtttgactgccttaggctggaaaatggtggagttcccattggaccctccacttgctaagatgttgttgatgggtgaagagctaggatgcgtagatgaaattttgacaattgtatccatgctttcagtgccatcagtattctttaggcccaaagatagggcagaggagagcgatgctgctcgggaaaaattttcagtcccagagtccgatcacctgacattctacaatgtttatcaacagtggaaacaacatcagtatcggggggactggtgcagtgaccattttttgcatgttaaggggttacaaaaggctagagacgttaggtcccagctgctagagattctgaagacgttgaaaattccactgacatcttgttggcctgatactgacgttgtgagaaaagctatttgctctgcatactttcaaaatgctgcaaagtttaagggtgtgggggagtatgttaattgccggactgggatgccatgccatttacacccgagcagtgctctttatggtatgggatgcacccctgagtatgtggtttatcatgaacagattctgacaacaaaggagtacatgcagtgtgtaacagcagtggagccgcagtggttggctgattggggacccatgttcttttctgtaaaggagtcggatacatcagtgttgcagaataagaagagacagcaggaagaaaaaacagctatggaggaggaaatggacaatctgagaaaggctcaagcacgggcagaggcagaaagcaagcagagggagagggaaaagaggtataaacagcagcagcagcagcaagtctcattggcgggtttgcaccagggctcttctacatatctgaggcccaagaaacttggcttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1331

Amino Acids

150.25

Weight (kDa)

6.02

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 876
AccB7I CCANNNNNTGG 1 cut(s) 529
AccI GTMKAC 1 cut(s) 636
AciI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
AclI AACGTT 1 cut(s) 1684
AclWI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
AcoI YGGCCR 4 cut(s) 675, 1608, 2542, 2949
AcsI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
AcuI CTGAAG 5 cut(s) 72, 834, 1008, 3035, 3488
AcvI CACGTG 1 cut(s) 526
AfiI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
AflIII ACRYGT 2 cut(s) 2056, 2903
AgeI ACCGGT 1 cut(s) 431
AhdI GACNNNNNGTC 2 cut(s) 1082, 3445
AjiI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
AjnI CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
Alw21I GWGCWC 4 cut(s) 135, 2395, 3109, 3631
Alw26I GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
Alw44I GTGCAC 1 cut(s) 2391
AlwI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
AlwNI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
Ama87I CYCGRG 3 cut(s) 1099, 3303, 3619
ApaLI GTGCAC 1 cut(s) 2391
ApoI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AsiGI ACCGGT 1 cut(s) 431
Asp700I GAANNNNTTC 2 cut(s) 3069, 3678
AspA2I CCTAGG 1 cut(s) 2810
AspLEI GCGC 2 cut(s) 877, 1398
AsuC2I CCSGG 1 cut(s) 2517
AsuHPI GGTGA 8 cut(s) 1166, 1196, 1727, 1796, 2126, 2531, 3209, 3326
AsuII TTCGAA 1 cut(s) 1667
AvaI CYCGRG 3 cut(s) 1099, 3303, 3619
AvaII GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
AvrII CCTAGG 1 cut(s) 2810
AxyI CCTNAGG 1 cut(s) 3136
BaeGI GKGCMC 1 cut(s) 2395
BalI TGGCCA 3 cut(s) 677, 1610, 2951
BamHI GGATCC 1 cut(s) 1591
BanII GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
BbrPI CACGTG 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 1083, 3476
Bbv12I GWGCWC 4 cut(s) 135, 2395, 3109, 3631
BbvCI CCTCAGC 1 cut(s) 651
BcgI CGANNNNNNTGC 2 cut(s) 1913, 1947
BciT130I CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BciVI GTATCC 3 cut(s) 2440, 3247, 3769
BcnI CCSGG 1 cut(s) 2517
BcoDI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BfaI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
BfmI CTRYAG 3 cut(s) 1767, 1922, 2475
BfuI GTATCC 3 cut(s) 2440, 3247, 3769
BglI GCCNNNNNGGC 2 cut(s) 674, 1067
BglII AGATCT 3 cut(s) 268, 724, 817
BlnI CCTAGG 1 cut(s) 2810
BlpI GCTNAGC 1 cut(s) 1818
BmcAI AGTACT 2 cut(s) 2193, 2441
Bme1390I CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
Bme18I GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
BmeRI GACNNNNNGTC 2 cut(s) 1082, 3445
BmeT110I CYCGRG 3 cut(s) 1099, 3303, 3619
BmgBI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
BmrFI CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
BmrI ACTGGG 2 cut(s) 2901, 3606
BmuI ACTGGG 2 cut(s) 2901, 3606
BpiI GAAGAC 2 cut(s) 1083, 3476
BplI GAGNNNNNCTC 8 cut(s) 1327, 1359, 1357, 1389, 3613, 3645, 3841, 3873
Bpu10I CCTNAGC 2 cut(s) 651, 846
Bpu1102I GCTNAGC 1 cut(s) 1818
Bpu14I TTCGAA 1 cut(s) 1667
BpuEI CTTGAG 4 cut(s) 128, 163, 2990, 3843
BpuMI CCSGG 1 cut(s) 2517
BsaAI YACGTR 3 cut(s) 236, 526, 2992
BsaJI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 4 cut(s) 1513, 1543, 3713, 3743
Bsc4I CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
Bse118I RCCGGY 3 cut(s) 431, 1573, 2540
Bse1I ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
Bse21I CCTNAGG 1 cut(s) 3136
BseBI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BseDI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BseLI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
BseNI ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
BseRI GAGGAG 8 cut(s) 611, 668, 1163, 2564, 2593, 2596, 3302, 3845
BseSI GKGCMC 1 cut(s) 2395
BseYI CCCAGC 4 cut(s) 2046, 2316, 2873, 3450
BsgI GTGCAG 5 cut(s) 772, 960, 1422, 1487, 3415
BshTI ACCGGT 1 cut(s) 431
BsiHKAI GWGCWC 4 cut(s) 135, 2395, 3109, 3631
BsiHKCI CYCGRG 3 cut(s) 1099, 3303, 3619
BsiSI CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
BslFI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
BslI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
BsmAI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BsmBI CGTCTC 1 cut(s) 3432
BsmFI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
BsmI GAATGC 2 cut(s) 267, 493
BsoBI CYCGRG 3 cut(s) 1099, 3303, 3619
Bsp119I TTCGAA 1 cut(s) 1667
Bsp1286I GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
Bsp1407I TGTACA 2 cut(s) 2428, 2916
Bsp1720I GCTNAGC 1 cut(s) 1818
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
BspHI TCATGA 3 cut(s) 1516, 2535, 3670
BspMAI CTGCAG 2 cut(s) 1926, 2479
BspPI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
BspQI GCTCTTC 3 cut(s) 2835, 3195, 3964
BspT104I TTCGAA 1 cut(s) 1667
BsrFI RCCGGY 3 cut(s) 431, 1573, 2540
BsrGI TGTACA 2 cut(s) 2428, 2916
BsrI ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
BssAI RCCGGY 3 cut(s) 431, 1573, 2540
BssECI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BssT1I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Bst2UI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
Bst6I CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
BstAPI GCANNNNNTGC 3 cut(s) 1409, 1763, 1814
BstAUI TGTACA 2 cut(s) 2428, 2916
BstBAI YACGTR 3 cut(s) 236, 526, 2992
BstBI TTCGAA 1 cut(s) 1667
BstDSI CCRYGG 1 cut(s) 396
BstHHI GCGC 2 cut(s) 877, 1398
BstMAI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BstNI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BstNSI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
BstSCI CCNGG 7 cut(s) 993, 1536, 2410, 2515, 2896, 3060, 3951
BstSFI CTRYAG 3 cut(s) 1767, 1922, 2475
BstSLI GKGCMC 1 cut(s) 2395
BstV2I GAAGAC 2 cut(s) 1083, 3476
BstX2I RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
BstXI CCANNNNNNTGG 1 cut(s) 2873
BstYI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
Bsu36I CCTNAGG 1 cut(s) 3136
BsuI GTATCC 3 cut(s) 2440, 3247, 3769
BtgI CCRYGG 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 3309
BtrI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
BtsI GCAGTG 7 cut(s) 1405, 2484, 3403, 3631, 3710, 3723, 3734
CaiI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
CciI TCATGA 3 cut(s) 1516, 2535, 3670
CfoI GCGC 2 cut(s) 877, 1398
Cfr10I RCCGGY 3 cut(s) 431, 1573, 2540
CseI GACGC 1 cut(s) 2309
CspAI ACCGGT 1 cut(s) 431
CspCI CAANNNNNGTGG 2 cut(s) 2040, 2075
DraI TTTAAA 1 cut(s) 298
DriI GACNNNNNGTC 2 cut(s) 1082, 3445
EaeI YGGCCR 4 cut(s) 675, 1608, 2542, 2949
Eam1104I CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
Eam1105I GACNNNNNGTC 2 cut(s) 1082, 3445
EarI CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
Ecl136II GAGCTC 1 cut(s) 3107
Eco130I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Eco147I AGGCCT 1 cut(s) 1528
Eco24I GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
Eco32I GATATC 1 cut(s) 2524
Eco47I GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
Eco53kI GAGCTC 1 cut(s) 3107
Eco57I CTGAAG 5 cut(s) 72, 834, 1008, 3035, 3488
Eco72I CACGTG 1 cut(s) 526
Eco81I CCTNAGG 1 cut(s) 3136
Eco88I CYCGRG 3 cut(s) 1099, 3303, 3619
EcoICRI GAGCTC 1 cut(s) 3107
EcoO109I RGGNCCY 2 cut(s) 3447, 3746
EcoRI GAATTC 1 cut(s) 1850
EcoRII CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
EcoRV GATATC 1 cut(s) 2524
EcoT14I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
EcoT22I ATGCAT 2 cut(s) 952, 2717
EcoT38I GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
ErhI CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Esp3I CGTCTC 1 cut(s) 3432
FalI AAGNNNNNCTT 6 cut(s) 1491, 1523, 1516, 1548, 2426, 2458
FaqI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
FauI CCCGC 2 cut(s) 1902, 3934
FauNDI CATATG 1 cut(s) 1824
FblI GTMKAC 1 cut(s) 636
FriOI GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
FspBI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
FspI TGCGCA 1 cut(s) 876
GlaI GCGC 2 cut(s) 876, 1397
GsaI CCCAGC 4 cut(s) 2050, 2320, 2877, 3454
HapII CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
HgaI GACGC 1 cut(s) 2309
HhaI GCGC 2 cut(s) 877, 1398
Hin6I GCGC 2 cut(s) 875, 1396
HinP1I GCGC 2 cut(s) 875, 1396
HincII GTYRAC 1 cut(s) 1682
HindII GTYRAC 1 cut(s) 1682
HinfI GANTC 8 cut(s) 29, 71, 1157, 2216, 3326, 3463, 3679, 3770
HpaII CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
HphI GGTGA 8 cut(s) 1166, 1196, 1727, 1796, 2126, 2531, 3209, 3326
Hpy166II GTNNAC 7 cut(s) 523, 637, 1682, 2026, 2393, 2803, 2919
Hpy8I GTNNAC 7 cut(s) 523, 637, 1682, 2026, 2393, 2803, 2919
Hpy99I CGWCG 2 cut(s) 2315, 2325
HpyAV CCTTC 4 cut(s) 1160, 1238, 1309, 3010
HspAI GCGC 2 cut(s) 875, 1396
KflI GGGWCCC 1 cut(s) 3746
LguI GCTCTTC 3 cut(s) 2835, 3195, 3964
LmnI GCTCC 6 cut(s) 4, 1033, 1570, 1693, 3104, 3721
MaeI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
MfeI CAATTG 1 cut(s) 3231
MflI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
MhlI GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
MlsI TGGCCA 3 cut(s) 677, 1610, 2951
MluNI TGGCCA 3 cut(s) 677, 1610, 2951
MlyI GAGTC 2 cut(s) 3335, 3779
MmeI TCCRAC 3 cut(s) 525, 1939, 3753
Mox20I TGGCCA 3 cut(s) 677, 1610, 2951
Mph1103I ATGCAT 2 cut(s) 952, 2717
MroXI GAANNNNTTC 2 cut(s) 3069, 3678
MscI TGGCCA 3 cut(s) 677, 1610, 2951
MslI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
Msp20I TGGCCA 3 cut(s) 677, 1610, 2951
MspA1I CMGCKG 6 cut(s) 248, 1256, 2656, 2663, 3091, 3454
MspI CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
MspR9I CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
MunI CAATTG 1 cut(s) 3231
Mva1269I GAATGC 2 cut(s) 267, 493
MvaI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
NciI CCSGG 1 cut(s) 2517
NcoI CCATGG 1 cut(s) 396
NdeI CATATG 1 cut(s) 1824
NmuCI GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
NsbI TGCGCA 1 cut(s) 876
NsiI ATGCAT 2 cut(s) 952, 2717
NspI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
NspV TTCGAA 1 cut(s) 1667
PaeI GCATGC 1 cut(s) 1195
PagI TCATGA 3 cut(s) 1516, 2535, 3670
PasI CCCWGGG 1 cut(s) 994
PceI AGGCCT 1 cut(s) 1528
PciI ACATGT 1 cut(s) 2903
PciSI GCTCTTC 3 cut(s) 2835, 3195, 3964
PcsI WCGNNNNNNNCGW 2 cut(s) 1328, 2875
PctI GAATGC 2 cut(s) 267, 493
PdmI GAANNNNTTC 2 cut(s) 3069, 3678
PfeI GAWTC 6 cut(s) 29, 71, 1157, 2216, 3463, 3679
PflMI CCANNNNNTGG 1 cut(s) 529
PfoI TCCNGGA 2 cut(s) 1536, 2410
PinAI ACCGGT 1 cut(s) 431
PleI GAGTC 2 cut(s) 3334, 3778
PmaCI CACGTG 1 cut(s) 526
PmlI CACGTG 1 cut(s) 526
PpsI GAGTC 2 cut(s) 3334, 3778
Ppu21I YACGTR 3 cut(s) 236, 526, 2992
PpuMI RGGWCCY 2 cut(s) 3447, 3746
PscI ACATGT 1 cut(s) 2903
Psp124BI GAGCTC 1 cut(s) 3109
Psp1406I AACGTT 1 cut(s) 1684
Psp5II RGGWCCY 2 cut(s) 3447, 3746
Psp6I CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
PspCI CACGTG 1 cut(s) 526
PspFI CCCAGC 4 cut(s) 2046, 2316, 2873, 3450
PspGI CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
PspPPI RGGWCCY 2 cut(s) 3447, 3746
PsrI GAACNNNNNNTAC 2 cut(s) 2972, 3004
PstI CTGCAG 2 cut(s) 1926, 2479
PstNI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
PsuI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
PvuII CAGCTG 4 cut(s) 2656, 2663, 3091, 3454
RseI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
SacI GAGCTC 1 cut(s) 3109
SapI GCTCTTC 3 cut(s) 2835, 3195, 3964
ScaI AGTACT 2 cut(s) 2193, 2441
SchI GAGTC 2 cut(s) 3335, 3779
ScrFI CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
SduI GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
SfcI CTRYAG 3 cut(s) 1767, 1922, 2475
SfuI TTCGAA 1 cut(s) 1667
SinI GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
SmiMI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
SmlI CTYRAG 4 cut(s) 107, 142, 3005, 3858
SmoI CTYRAG 4 cut(s) 107, 142, 3005, 3858
SphI GCATGC 1 cut(s) 1195
SseBI AGGCCT 1 cut(s) 1528
SsiI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
SspI AATATT 2 cut(s) 2731, 3070
SspMI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
SstI GAGCTC 1 cut(s) 3109
StuI AGGCCT 1 cut(s) 1528
StyD4I CCNGG 7 cut(s) 993, 1536, 2410, 2515, 2896, 3060, 3951
StyI CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
TaqI TCGA 6 cut(s) 129, 173, 1309, 1667, 1933, 3111
TatI WGTACW 7 cut(s) 412, 2175, 2191, 2428, 2439, 2916, 3696
TauI GCSGC 3 cut(s) 50, 248, 3727
TfiI GAWTC 6 cut(s) 29, 71, 1157, 2216, 3463, 3679
TseFI GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
Tsp45I GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
TspGWI ACGGA 1 cut(s) 1337
Van91I CCANNNNNTGG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 2391
VpaK11BI GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
XapI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
XceI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
XcmI CCANNNNNNNNNTGG 1 cut(s) 1603
XmaJI CCTAGG 1 cut(s) 2810
XmiI GTMKAC 1 cut(s) 636
XmnI GAANNNNTTC 2 cut(s) 3069, 3678
XspI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
ZrmI AGTACT 2 cut(s) 2193, 2441
Zsp2I ATGCAT 2 cut(s) 952, 2717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.