MD10G1223900.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002001
Physical Location & Seq
Reverse (-)
32177837 .. 32181226
3390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1223900.v1.1.491

Sequence Viewer

Length: 3390 bp
ATGGAACAAAAAAATAGCAGTGGTGGTCGGGGATTATATGTTTCAGGGAAGGACAGAGTTGTTTTTAGGCGTCCGGGGAAGAAATCTGTCTTGGGTTTCGATGTCCATGCTAATGCGAAACGGGAAGGGTTTGGGGGCGGTGATGGCGGATTCGAGGCACCGAGAGATTGTGAGGGTAGGTATGATTACGACAGGGAAGAGCATGGTAGTAGGAGGAGAGATTGCAAAGATGGTAGTGGTAGTTATGGTAAGGATTCTTGTCGTCGAAGGAAAGCAAGTGACGGGTATGAACATGATCAATGTGGTGGACAATATGGAAGAAAGCGGAGTAGATGTAATTTTTCTGGTTCTGATGCTTCTCCGTGGGATCTCATCTCATCGTCTCCAGTTCGAATTTGTCCTTCTGGAGACGCGGTTAAATTTTCAAGTTCGAAACATGGTGTGAGTTCTCGTAAAGTTGCCTTTCACTCAGAAAATTCACAATCATTCAAGGATGGAGAAGGAGACATGGCTGATTTGGATGAAGGACAGAAATATGAGATAAGCGAAAGCATGCGTCTAGAGATGGAATACAATTCTGACCGAGCATGGTATGATAGGGAAGAAGGAAACATAATGTTCGATACAGTTGATAGCTTATCTTTGTTTTACGGAGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCGAAAAGATTGGTTCGAAGAGATGGAACTAAGATGACCCTTGCTCAGAGCAAAAAGTTGTCTCAGCTCACTGCTGATACTGCGCGGTGGGAGGATCGTCAACTTATAAGATCTGGTGCTGTTAGAGGGACTGGGTTGCAGACTGAGCTCGATGATGAAGAAGAGCACAAAGTTATTCTTCTTGTACATGATACAAATTTAAAGCCTCCTTTCCTTGAGGACAGTGTAATTGGAAAGGACGGAACTGCTGTTTTTACCAGGCGAGCAGAGACGATAATGCCAATAAAGGATTCCACATCCGATATGGCCATAATTTCTCGCAAAGGGTCTGCTCTCGTTAGGAAAATTCATGAGAAGCAAACTATGAACAAATCGCGTCAACGTTTTTGGGAGCTTGCAGGTTCTAAACTAGGCGATATCCTTGGTGTTGAGAAAACAGCGGAGCAGATTGATGCAGATACTGCTGCTGCTGGTGCAGACGGTGAAATTGATTTCAAAGAAGATGCAAAGTTTGCACAGCATATGAAGGATGGGGAAGCAGCGAGTGAATTCGCAAAGTCAAAAACCATCTCACAGCAACGACAGTTTTTGCCCATATATTCTGTGAGGGATGATTTACTACAGGTAATTCGAGAAAATCAAGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACGACTCAACTGACACAGTATCTGCACGAAGAGGGTTACACTGTAAACGGCATTGTGGGTTGCACGCAACCAAGACGTGTGGCAGCCATGAGTGTTGCAAAGAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGATAAAGTTGGGTACGCCATTCGTTTTGAGGATGTGACCGGACCAAATACCATAATAAAGTACATGACTGATGGAGTACTTATGCGCGAAACACTTAGAGACTCTGATCTTGACAAGTATCACGTAGTTGTGATGGACGAAGCCCATGAAAGGTCACTGAACACGGATGTGCTGCTTGGGATTTTGAAAACAGTGGTTGCCCGGCGGCGTGATTTTAAGCTTATCGTGACATCTGCAACACTAGATGCTCAGAAGTTTAGAAACCTTTTTGGAAGTGCACCGATTTTTGAAATTCCTGGGAGAACATTTCCAGTAAGTATCAGGTATAGTAAAACCCCATGTGAAGATTATGTTGAAGCTGCAGTGAAGGAAGCCATAACTATTCACATCACTGGCACTCAAGGTGACATCCTCATCTTCATGACCGGCCAGGATGAGATCGAGGCAGCTTGTTATGCCCTTTCAGAGCGGATGGAACAAATTATCTCCACCTCGGTTAAAGAGGTGCCAAAACTCTTGATACTCCCTATATATTCACAGTTGCCTGCTGACTTGCAAGCAAAGATATTCCAAAAAGCTGAAGATGGGGCTCGTAAATGTATTGTGGCAACCAACATTGCTGAGACATCTTTGACGGTAGATGGAATCTCATATGTCATCGACACGGGATATGGTAAAATGAAAGTATACAACCCCAAAATGGGTATGGATGCTCTTCAAGTATTTCCTATTAGTCAGACTGCTGCTAGCCAACGTGCTGGGCGTGCTGGCAGAACTAAGCCTGGAACATGTTATCGGCTGTACACAGAGAATGCGTACTTAAATGAAATGCTGCCAAATCCTGTGCCAGAGATCCAGAGGACCAACCTCGGATATGTGGTCTTGTTGCTCAAATCACTTCAGGTTGATAAGATACTAGATTTTGATTTCGTAGACCCACCGCCCAAGGATAATATTCTCCATTCTATGTACCAGTTGTGGGTGTTGGGTGCTCTTAACAATGAGGGGCGGTTAACTGACCTGGGCTGGAAAATGGTGGAATTGCCGTTGGACCCTCCACTCGCTAAAATGCTATTGATGGGTGAAAAGCTAGGATGTTTAGATGAGGTTTTGACAATTGTGTCCATGCTTTCAGTGCCGTCGGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGTGATGCTGCAAGGGAGAAATTCTCCTTCCCAGAGTCCGACCACTTGACACTGTACAATGTTTATCAGCAGTGGGAACGACACCAGTGTCGTGGGGACTGGTGTGATGACCACTTTTTGCAAGTTAAAGGGCTGCGAAAGGTTAGAGAGGTGAGGTCCCAACTCCTAGAGATACTAAAGACACTCAAGGTCAAAGTCCCACTCACATCCTGTTGGCCTGATACTGATAAGGTGAGACAAGCTATTTGCTCCGCGTACTTTCAGAATGCGGCAAGACTAAAGGGTGTGGGGGAGTATGTTAACTGCCGGACTGGAATGCCATGCTATTTACACCCGAGCAGTGCGCTTTATGGCATGGGCTGCAATCCAGAGTATGTGGTCTATCATGAGTTAGTTTTGACAAGGAAGGAGTATATGCAGTGTGCCACTGCGGTGGAACCGCAATGGTTGGCTGAGTCCGGACCCATGTTCTATTATGTGAAGGAGTCGGATAACTCAATGTTGGAGCACAAGAAGAGACAAAAGGGGGAAAAGACAGCAATGCAGGCGGAAATGGAGAATCTGAGAAGGGCTCGAGCAGAGGCAGAGAAAGCAAGCAAGCAGAAGGAGAGGGAAAAGAGATCCAAGCAGCAGCAGCAGCAAGTCGTCCCAATGCTGGGTTCGCACCAGGGCTCTTCTGCATACTTGAGGCCGAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1130

Amino Acids

126.93

Weight (kDa)

6.76

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 437 - 586 6.7e-08 DEAD/DEAH box helicase
AAA_22 PF13401 442 - 576 5.4e-06 AAA domain
Helicase_C PF00271 632 - 760 3.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 826 - 851 8.2e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 852 - 911 1.5e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 971 - 1048 4.7e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
AasI GACNNNNNNGTC 2 cut(s) 2623, 2766
Acc36I ACCTGC 1 cut(s) 1079
AccB1I GGYRCC 2 cut(s) 157, 2008
AccBSI CCGCTC 1 cut(s) 1972
AccI GTMKAC 2 cut(s) 2190, 2436
AccII CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
AccIII TCCGGA 1 cut(s) 3137
AclI AACGTT 1 cut(s) 1072
AclWI GGATC 4 cut(s) 375, 792, 2350, 3294
AcoI YGGCCR 2 cut(s) 996, 1930
AcsI RAATTY 8 cut(s) 393, 419, 475, 886, 1035, 1238, 1794, 2699
AcuI CTGAAG 2 cut(s) 2103, 2387
AcyI GRCGYC 1 cut(s) 70
AfaI GTAC 9 cut(s) 876, 1517, 1565, 1581, 2306, 2321, 2474, 2735, 2936
AfiI CCNNNNNNNGG 7 cut(s) 1653, 2203, 2204, 2482, 2667, 3334, 3335
AflIII ACRYGT 2 cut(s) 1444, 2291
AgsI TTSAA 8 cut(s) 426, 490, 671, 1186, 1690, 1793, 1859, 2222
AjiI CACGTC 1 cut(s) 1445
AjnI CCWGG 6 cut(s) 947, 1798, 1932, 2284, 2523, 3345
AjuI GAANNNNNNNTTGG 6 cut(s) 74, 106, 964, 996, 3322, 3354
AleI CACNNNNGTG 2 cut(s) 1670, 3110
Alw21I GWGCWC 5 cut(s) 840, 858, 1783, 2497, 3189
Alw44I GTGCAC 1 cut(s) 1779
AlwI GGATC 4 cut(s) 375, 792, 2350, 3294
AlwNI CAGNNNCTG 2 cut(s) 1151, 1390
Ama87I CYCGRG 2 cut(s) 3013, 3252
Aor13HI TCCGGA 1 cut(s) 3137
AoxI GGCC 5 cut(s) 996, 1930, 2657, 2894, 3368
ApaLI GTGCAC 1 cut(s) 1779
ApoI RAATTY 8 cut(s) 393, 419, 475, 886, 1035, 1238, 1794, 2699
ArsI GACNNNNNNTTYG 2 cut(s) 2907, 2939
AspLEI GCGC 3 cut(s) 775, 1590, 3025
AspS9I GGNCC 6 cut(s) 1544, 2364, 2554, 2658, 2835, 3140
AsuC2I CCSGG 2 cut(s) 75, 1705
AsuHPI GGTGA 7 cut(s) 152, 1184, 1514, 1919, 2597, 2842, 2923
AsuII TTCGAA 3 cut(s) 391, 431, 706
AsuNHI GCTAGC 1 cut(s) 2249
AvaI CYCGRG 2 cut(s) 3013, 3252
AvaII GGWCC 5 cut(s) 1544, 2364, 2554, 2835, 3140
BaeGI GKGCMC 1 cut(s) 1783
BalI TGGCCA 1 cut(s) 998
BanI GGYRCC 2 cut(s) 157, 2008
BanII GRGCYC 4 cut(s) 840, 2095, 3253, 3353
Bbv12I GWGCWC 5 cut(s) 840, 858, 1783, 2497, 3189
BceAI ACGGC 3 cut(s) 1432, 2533, 2626
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 6 cut(s) 949, 1800, 1934, 2286, 2525, 3347
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 2 cut(s) 75, 1705
BfaI CTAG 7 cut(s) 560, 1100, 1745, 2250, 2420, 2594, 2846
BfmI CTRYAG 2 cut(s) 1310, 1863
BfuAI ACCTGC 1 cut(s) 1079
BglII AGATCT 1 cut(s) 800
BmcAI AGTACT 1 cut(s) 1581
Bme1390I CCNGG 8 cut(s) 75, 949, 1705, 1800, 1934, 2286, 2525, 3347
Bme18I GGWCC 5 cut(s) 1544, 2364, 2554, 2835, 3140
BmeT110I CYCGRG 2 cut(s) 3013, 3252
BmgBI CACGTC 1 cut(s) 1445
BmgT120I GGNCC 6 cut(s) 1544, 2364, 2554, 2658, 2835, 3140
BmiI GGNNCC 6 cut(s) 159, 2010, 2556, 2837, 3117, 3142
BmrFI CCNGG 8 cut(s) 75, 949, 1705, 1800, 1934, 2286, 2525, 3347
BmrI ACTGGG 1 cut(s) 831
BmsI GCATC 7 cut(s) 343, 649, 1132, 1183, 1738, 2203, 2674
BmtI GCTAGC 1 cut(s) 2253
BmuI ACTGGG 1 cut(s) 831
BplI GAGNNNNNCTC 4 cut(s) 2687, 2719, 3235, 3267
BpmI CTGGAG 2 cut(s) 369, 426
Bpu14I TTCGAA 3 cut(s) 391, 431, 706
BpuEI CTTGAG 4 cut(s) 926, 1887, 2849, 3385
BpuMI CCSGG 2 cut(s) 75, 1705
BsaAI YACGTR 1 cut(s) 1627
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 9 cut(s) 74, 362, 1111, 1799, 1995, 2371, 2448, 2524, 3346
BsaWI WCCGGW 2 cut(s) 1541, 3137
BsaXI ACNNNNNCTCC 4 cut(s) 319, 349, 399, 429
Bsc4I CCNNNNNNNGG 7 cut(s) 1653, 2203, 2204, 2482, 2667, 3334, 3335
Bse118I RCCGGY 1 cut(s) 1928
Bse1I ACTGG 9 cut(s) 386, 826, 1360, 1814, 1900, 2476, 2764, 2783, 2995
Bse3DI GCAATG 3 cut(s) 2118, 3128, 3225
BseAI TCCGGA 1 cut(s) 3137
BseBI CCWGG 6 cut(s) 949, 1800, 1934, 2286, 2525, 3347
BseDI CCNNGG 9 cut(s) 74, 362, 1111, 1799, 1995, 2371, 2448, 2524, 3346
BseLI CCNNNNNNNGG 7 cut(s) 1653, 2203, 2204, 2482, 2667, 3334, 3335
BseMI GCAATG 3 cut(s) 2118, 3128, 3225
BseMII CTCAG 9 cut(s) 483, 749, 767, 825, 1485, 1766, 2115, 3123, 3233
BseNI ACTGG 9 cut(s) 386, 826, 1360, 1814, 1900, 2476, 2764, 2783, 2995
BseRI GAGGAG 2 cut(s) 229, 2690
BseSI GKGCMC 1 cut(s) 1783
BseYI CCCAGC 2 cut(s) 2261, 3334
BsgI GTGCAG 2 cut(s) 1185, 1376
Bsh1236I CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
BshFI GGCC 5 cut(s) 998, 1932, 2659, 2896, 3370
BshNI GGYRCC 2 cut(s) 157, 2008
BsiHKAI GWGCWC 5 cut(s) 840, 858, 1783, 2497, 3189
BsiHKCI CYCGRG 2 cut(s) 3013, 3252
BsiSI CCGG 6 cut(s) 74, 1542, 1705, 1929, 2986, 3138
BslFI GGGAC 5 cut(s) 832, 2789, 2821, 2861, 3311
BslI CCNNNNNNNGG 7 cut(s) 1653, 2203, 2204, 2482, 2667, 3334, 3335
BsmBI CGTCTC 3 cut(s) 387, 402, 953
BsmFI GGGAC 5 cut(s) 832, 2789, 2821, 2861, 3311
BsmI GAATGC 3 cut(s) 2320, 2950, 3000
BsnI GGCC 5 cut(s) 998, 1932, 2659, 2896, 3370
BsoBI CYCGRG 2 cut(s) 3013, 3252
Bsp119I TTCGAA 3 cut(s) 391, 431, 706
Bsp1286I GDGCHC 8 cut(s) 840, 858, 1783, 2095, 2497, 3189, 3253, 3353
Bsp13I TCCGGA 1 cut(s) 3137
Bsp1407I TGTACA 3 cut(s) 874, 2304, 2733
Bsp143I GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
BspANI GGCC 5 cut(s) 998, 1932, 2659, 2896, 3370
BspCNI CTCAG 9 cut(s) 482, 748, 766, 826, 1486, 1765, 2116, 3124, 3234
BspEI TCCGGA 1 cut(s) 3137
BspFNI CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
BspHI TCATGA 3 cut(s) 1039, 1923, 3064
BspLI GGNNCC 6 cut(s) 159, 2010, 2556, 2837, 3117, 3142
BspMAI CTGCAG 1 cut(s) 1867
BspMI ACCTGC 1 cut(s) 1079
BspOI GCTAGC 1 cut(s) 2253
BspPI GGATC 4 cut(s) 375, 792, 2350, 3294
BspQI GCTCTTC 4 cut(s) 192, 846, 2223, 3358
BspT104I TTCGAA 3 cut(s) 391, 431, 706
BspT107I GGYRCC 2 cut(s) 157, 2008
BsrBI CCGCTC 1 cut(s) 1972
BsrDI GCAATG 3 cut(s) 2118, 3128, 3225
BsrFI RCCGGY 1 cut(s) 1928
BsrGI TGTACA 3 cut(s) 874, 2304, 2733
BsrI ACTGG 9 cut(s) 386, 826, 1360, 1814, 1900, 2476, 2764, 2783, 2995
BssAI RCCGGY 1 cut(s) 1928
BssECI CCNNGG 9 cut(s) 74, 362, 1111, 1799, 1995, 2371, 2448, 2524, 3346
BssMI GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
BssNAI GTATAC 1 cut(s) 2191
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 2 cut(s) 1111, 2448
Bst1107I GTATAC 1 cut(s) 2191
Bst2UI CCWGG 6 cut(s) 949, 1800, 1934, 2286, 2525, 3347
Bst6I CTCTTC 8 cut(s) 192, 703, 846, 1392, 1476, 2223, 3188, 3358
BstACI GRCGYC 1 cut(s) 70
BstAPI GCANNNNNTGC 3 cut(s) 1151, 1202, 3039
BstAUI TGTACA 3 cut(s) 874, 2304, 2733
BstBAI YACGTR 1 cut(s) 1627
BstBI TTCGAA 3 cut(s) 391, 431, 706
BstDSI CCRYGG 1 cut(s) 362
BstFNI CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
BstHHI GCGC 3 cut(s) 775, 1590, 3025
BstKTI GATC 8 cut(s) 298, 370, 787, 803, 1612, 1944, 2358, 3302
BstMBI GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
BstNI CCWGG 6 cut(s) 949, 1800, 1934, 2286, 2525, 3347
BstNSI RCATGY 2 cut(s) 556, 2295
BstSCI CCNGG 8 cut(s) 73, 947, 1703, 1798, 1932, 2284, 2523, 3345
BstSFI CTRYAG 2 cut(s) 1310, 1863
BstSLI GKGCMC 1 cut(s) 1783
BstUI CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
BstX2I RGATCY 4 cut(s) 367, 800, 2355, 3299
BstXI CCANNNNNNTGG 3 cut(s) 2261, 2771, 3112
BstYI RGATCY 4 cut(s) 367, 800, 2355, 3299
BstZ17I GTATAC 1 cut(s) 2191
BsuRI GGCC 5 cut(s) 998, 1932, 2659, 2896, 3370
BtgI CCRYGG 1 cut(s) 362
BtrI CACGTC 1 cut(s) 1445
BtsI GCAGTG 7 cut(s) 25, 759, 1872, 2756, 3025, 3104, 3105
BveI ACCTGC 1 cut(s) 1079
CaiI CAGNNNCTG 2 cut(s) 1151, 1390
CciI TCATGA 3 cut(s) 1039, 1923, 3064
CfoI GCGC 3 cut(s) 775, 1590, 3025
Cfr10I RCCGGY 1 cut(s) 1928
Cfr13I GGNCC 6 cut(s) 1544, 2364, 2554, 2658, 2835, 3140
CseI GACGC 4 cut(s) 59, 419, 545, 1055
Csp6I GTAC 9 cut(s) 875, 1516, 1564, 1580, 2305, 2320, 2473, 2734, 2935
CspCI CAANNNNNGTGG 2 cut(s) 1428, 1463
CviQI GTAC 9 cut(s) 875, 1516, 1564, 1580, 2305, 2320, 2473, 2734, 2935
DpnI GATC 8 cut(s) 297, 369, 786, 802, 1611, 1943, 2357, 3301
DpnII GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
DraI TTTAAA 1 cut(s) 891
DrdI GACNNNNNNGTC 2 cut(s) 2623, 2766
DseDI GACNNNNNNGTC 2 cut(s) 2623, 2766
EaeI YGGCCR 2 cut(s) 996, 1930
Eam1104I CTCTTC 8 cut(s) 192, 703, 846, 1392, 1476, 2223, 3188, 3358
EarI CTCTTC 8 cut(s) 192, 703, 846, 1392, 1476, 2223, 3188, 3358
EciI GGCGGA 2 cut(s) 162, 3242
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 2 cut(s) 1111, 2448
Eco24I GRGCYC 4 cut(s) 840, 2095, 3253, 3353
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 5 cut(s) 1544, 2364, 2554, 2835, 3140
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 2103, 2387
Eco88I CYCGRG 2 cut(s) 3013, 3252
EcoICRI GAGCTC 1 cut(s) 838
EcoO109I RGGNCCY 1 cut(s) 2835
EcoRI GAATTC 1 cut(s) 1238
EcoRII CCWGG 6 cut(s) 947, 1798, 1932, 2284, 2523, 3345
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 2 cut(s) 1111, 2448
EcoT38I GRGCYC 4 cut(s) 840, 2095, 3253, 3353
ErhI CCWWGG 2 cut(s) 1111, 2448
Esp3I CGTCTC 3 cut(s) 387, 402, 953
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 5 cut(s) 832, 2789, 2821, 2861, 3311
FauNDI CATATG 2 cut(s) 1212, 2155
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 2 cut(s) 2190, 2436
FriOI GRGCYC 4 cut(s) 840, 2095, 3253, 3353
FspBI CTAG 7 cut(s) 560, 1100, 1745, 2250, 2420, 2594, 2846
GlaI GCGC 3 cut(s) 774, 1589, 3024
GsaI CCCAGC 2 cut(s) 2265, 3338
GsuI CTGGAG 2 cut(s) 369, 426
HaeIII GGCC 5 cut(s) 998, 1932, 2659, 2896, 3370
HapII CCGG 6 cut(s) 74, 1542, 1705, 1929, 2986, 3138
HgaI GACGC 4 cut(s) 59, 419, 545, 1055
HhaI GCGC 3 cut(s) 775, 1590, 3025
Hin1I GRCGYC 1 cut(s) 70
Hin6I GCGC 3 cut(s) 773, 1588, 3023
HinP1I GCGC 3 cut(s) 773, 1588, 3023
HincII GTYRAC 4 cut(s) 791, 1070, 2517, 2980
HindII GTYRAC 4 cut(s) 791, 1070, 2517, 2980
HindIII AAGCTT 1 cut(s) 1721
HpaI GTTAAC 2 cut(s) 2517, 2980
HpaII CCGG 6 cut(s) 74, 1542, 1705, 1929, 2986, 3138
HphI GGTGA 7 cut(s) 152, 1184, 1514, 1919, 2597, 2842, 2923
Hpy99I CGWCG 2 cut(s) 267, 2647
HpyCH4IV ACGT 4 cut(s) 1072, 1444, 1626, 2257
HpySE526I ACGT 4 cut(s) 1072, 1444, 1626, 2257
Hsp92I GRCGYC 1 cut(s) 70
HspAI GCGC 3 cut(s) 773, 1588, 3023
Kpn2I TCCGGA 1 cut(s) 3137
Ksp22I TGATCA 1 cut(s) 295
KspAI GTTAAC 2 cut(s) 2517, 2980
Kzo9I GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
LguI GCTCTTC 4 cut(s) 192, 846, 2223, 3358
LmnI GCTCC 4 cut(s) 1081, 1132, 2933, 3184
LweI GCATC 7 cut(s) 343, 649, 1132, 1183, 1738, 2203, 2674
MaeI CTAG 7 cut(s) 560, 1100, 1745, 2250, 2420, 2594, 2846
MaeII ACGT 4 cut(s) 1072, 1444, 1626, 2257
MaeIII GTNAC 6 cut(s) 278, 1403, 1537, 1656, 1729, 1907
MalI GATC 8 cut(s) 297, 369, 786, 802, 1611, 1943, 2357, 3301
MbiI CCGCTC 1 cut(s) 1972
MboI GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
MfeI CAATTG 1 cut(s) 2619
MflI RGATCY 4 cut(s) 367, 800, 2355, 3299
MhlI GDGCHC 8 cut(s) 840, 858, 1783, 2095, 2497, 3189, 3253, 3353
MlsI TGGCCA 1 cut(s) 998
MluNI TGGCCA 1 cut(s) 998
MlyI GAGTC 5 cut(s) 1366, 1598, 2723, 3143, 3173
MmeI TCCRAC 5 cut(s) 1327, 2532, 2742, 3147, 3162
Mox20I TGGCCA 1 cut(s) 998
MroI TCCGGA 1 cut(s) 3137
MscI TGGCCA 1 cut(s) 998
MseI TTAA 9 cut(s) 417, 890, 1719, 2001, 2324, 2499, 2516, 2805, 2979
MslI CAYNNNNRTG 4 cut(s) 111, 1670, 1922, 3110
Msp20I TGGCCA 1 cut(s) 998
MspA1I CMGCKG 1 cut(s) 1130
MspI CCGG 6 cut(s) 74, 1542, 1705, 1929, 2986, 3138
MspR9I CCNGG 8 cut(s) 75, 949, 1705, 1800, 1934, 2286, 2525, 3347
MunI CAATTG 1 cut(s) 2619
Mva1269I GAATGC 3 cut(s) 2320, 2950, 3000
MvaI CCWGG 6 cut(s) 949, 1800, 1934, 2286, 2525, 3347
MvnI CGCG 5 cut(s) 413, 775, 1066, 1590, 2933
NciI CCSGG 2 cut(s) 75, 1705
NdeI CATATG 2 cut(s) 1212, 2155
NdeII GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
NheI GCTAGC 1 cut(s) 2249
NlaIV GGNNCC 6 cut(s) 159, 2010, 2556, 2837, 3117, 3142
NmuCI GTSAC 5 cut(s) 278, 1537, 1656, 1729, 1907
NspI RCATGY 2 cut(s) 556, 2295
NspV TTCGAA 3 cut(s) 391, 431, 706
OliI CACNNNNGTG 2 cut(s) 1670, 3110
PaeI GCATGC 1 cut(s) 556
PaeR7I CTCGAG 1 cut(s) 3252
PagI TCATGA 3 cut(s) 1039, 1923, 3064
PciI ACATGT 1 cut(s) 2291
PciSI GCTCTTC 4 cut(s) 192, 846, 2223, 3358
PcsI WCGNNNNNNNCGW 1 cut(s) 2263
PctI GAATGC 3 cut(s) 2320, 2950, 3000
PfeI GAWTC 5 cut(s) 150, 254, 980, 2148, 3238
PleI GAGTC 5 cut(s) 1366, 1598, 2722, 3142, 3172
PpsI GAGTC 5 cut(s) 1366, 1598, 2722, 3142, 3172
Ppu21I YACGTR 1 cut(s) 1627
PpuMI RGGWCCY 1 cut(s) 2835
PscI ACATGT 1 cut(s) 2291
PsiI TTATAA 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 840
Psp1406I AACGTT 1 cut(s) 1072
Psp5II RGGWCCY 1 cut(s) 2835
Psp6I CCWGG 6 cut(s) 947, 1798, 1932, 2284, 2523, 3345
PspFI CCCAGC 2 cut(s) 2261, 3334
PspGI CCWGG 6 cut(s) 947, 1798, 1932, 2284, 2523, 3345
PspN4I GGNNCC 6 cut(s) 159, 2010, 2556, 2837, 3117, 3142
PspPI GGNCC 6 cut(s) 1544, 2364, 2554, 2658, 2835, 3140
PspPPI RGGWCCY 1 cut(s) 2835
PspXI VCTCGAGB 1 cut(s) 3252
PstI CTGCAG 1 cut(s) 1867
PstNI CAGNNNCTG 2 cut(s) 1151, 1390
PsuI RGATCY 4 cut(s) 367, 800, 2355, 3299
RsaI GTAC 9 cut(s) 876, 1517, 1565, 1581, 2306, 2321, 2474, 2735, 2936
RsaNI GTAC 9 cut(s) 875, 1516, 1564, 1580, 2305, 2320, 2473, 2734, 2935
RseI CAYNNNNRTG 4 cut(s) 111, 1670, 1922, 3110
SacI GAGCTC 1 cut(s) 840
SapI GCTCTTC 4 cut(s) 192, 846, 2223, 3358
SaqAI TTAA 9 cut(s) 417, 890, 1719, 2001, 2324, 2499, 2516, 2805, 2979
Sau3AI GATC 8 cut(s) 295, 367, 784, 800, 1609, 1941, 2355, 3299
Sau96I GGNCC 6 cut(s) 1544, 2364, 2554, 2658, 2835, 3140
ScaI AGTACT 1 cut(s) 1581
SchI GAGTC 5 cut(s) 1366, 1598, 2723, 3143, 3173
ScrFI CCNGG 8 cut(s) 75, 949, 1705, 1800, 1934, 2286, 2525, 3347
SduI GDGCHC 8 cut(s) 840, 858, 1783, 2095, 2497, 3189, 3253, 3353
SfaNI GCATC 7 cut(s) 343, 649, 1132, 1183, 1738, 2203, 2674
SfcI CTRYAG 2 cut(s) 1310, 1863
Sfr274I CTCGAG 1 cut(s) 3252
SfuI TTCGAA 3 cut(s) 391, 431, 706
SinI GGWCC 5 cut(s) 1544, 2364, 2554, 2835, 3140
SlaI CTCGAG 1 cut(s) 3252
SmiMI CAYNNNNRTG 4 cut(s) 111, 1670, 1922, 3110
SmlI CTYRAG 5 cut(s) 905, 1902, 2864, 3252, 3364
SmoI CTYRAG 5 cut(s) 905, 1902, 2864, 3252, 3364
SphI GCATGC 1 cut(s) 556
SspI AATATT 1 cut(s) 2458
SspMI CTAG 7 cut(s) 560, 1100, 1745, 2250, 2420, 2594, 2846
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 8 cut(s) 73, 947, 1703, 1798, 1932, 2284, 2523, 3345
StyI CCWWGG 2 cut(s) 1111, 2448
TaiI ACGT 4 cut(s) 1075, 1447, 1629, 2260
TaqII GACCGA 1 cut(s) 597
TatI WGTACW 5 cut(s) 874, 1563, 1579, 2304, 2733
TauI GCSGC 2 cut(s) 1711, 2951
TfiI GAWTC 5 cut(s) 150, 254, 980, 2148, 3238
Tru1I TTAA 9 cut(s) 417, 890, 1719, 2001, 2324, 2499, 2516, 2805, 2979
Tru9I TTAA 9 cut(s) 417, 890, 1719, 2001, 2324, 2499, 2516, 2805, 2979
TseFI GTSAC 5 cut(s) 278, 1537, 1656, 1729, 1907
Tsp45I GTSAC 5 cut(s) 278, 1537, 1656, 1729, 1907
TspGWI ACGGA 4 cut(s) 351, 666, 945, 1682
VneI GTGCAC 1 cut(s) 1779
VpaK11BI GGWCC 5 cut(s) 1544, 2364, 2554, 2835, 3140
XapI RAATTY 8 cut(s) 393, 419, 475, 886, 1035, 1238, 1794, 2699
XbaI TCTAGA 1 cut(s) 559
XceI RCATGY 2 cut(s) 556, 2295
XcmI CCANNNNNNNNNTGG 2 cut(s) 991, 2206
XhoI CTCGAG 1 cut(s) 3252
XmiI GTMKAC 2 cut(s) 2190, 2436
XspI CTAG 7 cut(s) 560, 1100, 1745, 2250, 2420, 2594, 2846
ZrmI AGTACT 1 cut(s) 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.