RLG00000032645

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002001
Physical Location & Seq
Forward (+)
17276742 .. 17280005
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032645

Sequence Viewer

Length: 3264 bp
ATGGTGAAATCGGAACTCGAGAAGAGCGGAGGAGGTGGAGGATTACATGTTCATGGGACGGAGAGAGTTGTTTTCAGGCCGCCGGAGAAGAAGAAATTAGGGTTTGATGGAGGTGCCAGGAGATATAAGGGTAGGTGTGATGATGGGGATGGTAGTGAAAGAAGAGATTACAGAGATGGTGGTGGTACTAGTAGGAGTAGGGTGAGAGGAAGATATGAAGAAGATGGATATGGTGGAGATTATGGAAGAAAGAAGAGTAGATATGAAGGTTCGAAGAGGGGAGCTCAATCTGTTGCTTCTGCCTGGGATTGTATCTCTTACTCTCCGGTTCAGATACGGGCTTCTGGACTGTTGGTCAAGTCTACAAGTTCGAGACGTGGCGGGAGTTCTCATCAGCTTGATAGTACACTAGGAGAAAAGAAGGGTGATTCGGTTGAAGGGAGATATGAGATTAGTGAGAGCATGCGTTTAGAGATGGAATATGATTGTGACCGTGCGTGGTATGATAGAGAAGAAGGAAGCACAGCGGTTGATATGAGTGATAGTTCGTCTTTGTTTTATGGGGATGATGCTTTTTGTCGAAAGAAGGAAACAGAGTTGGCCAAGAGACTGGTTCGGAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAGGTTGTCTCAGCGTACTGCAGATAATGCTCAGTGGGAGGATCGTCAGCTTCTGCGATCTGGTGCTGTTAGAGGTACTGAGGTGCAGAGTGAGATTGATGATGAAGATGAGAACAAAGTTATGCTTCTTGTTCATGATACAAGGCCTCCTTTCCTAGATGGGAGAGTTGTTTTTACCGAGCAAGCGGAGCCGGTTATGCCAGTAAAGGATCCCACGTCTGATATGGCCATAATTTCACGTAAAGGGTCTGCTCTAGTTAGGGAAATCCATCAAAAGCAAAGTTCGAATAAATCACGTCAACGTTTTTGGGAGCTTGCAGGTTCCAAACTTGGTGATATTCTTGGTGTTGAAAAAACAGCAGAGCATTTTAATGCAGATACTGTTGTAGTAGGTAAAGACGGTGAAGTTGATTTCAAAGAAGATGCAAAGTTTGCCCAGCATATGAAGAATGGGGAGGCTGTGAGTGCATTCACAAAGTCAAAAACCATTTCCGAACAACGCCAGTATCTGCCTATATATTCTGTGCGGGATGAGTTACTGCAGAAAATTCGAGAAAATCAGGTTATTATAGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGTTACACTGTGAACGGCATAGTCGGTTGCACCCAGCCAAGGCGTGTGGCAGCGATGAGTGTTGCTAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGTGAGAAAGTTGGCTATGCTATTCGTTTTGAGGACGTGACGGGGCCAAAAACTGTAATAAAGTACATGACTGATGGAGTACTTATGCGTGAAACACTTAGAGATTCTGACCTTGACAAGTACCGCGTAGTTGTAATGGATGAAGCCCATGAGAGGTCACTATATACAGATGTGCTGTTTGGGATTTTGAAAAAAGTCGTTGCCCAGCGTCGTGATTTTAAGCTCATTGTGACATCTGCTACACTTAATGCTCAGAAATTTGCAGATTTCTTTGGAGGTGCACCTATTTTTCCCATTCCTGGAAGAACATTTCCTGTACATAAGAAGTACTTGGATACCCCATGTGAAGATTATGTTGAAGCTGCAGTGACACAGGCCATGAAAATTCACATTGCCAGCCCTCCAGGTGATATCCTCATCTTCATGACCGGCCAAGAGGAGATTGAAGCAGCCTGTTATTCTCTCGCAGAGCGAATGGAACAACTTATCTCCTCCTCAAAGAAAGGGATGTCAAAGCTCTTGATACTCCCTATGTATTCACAGCTGCCAGCTGACTTGCAAGCAAAGATATTCCAAAGGTCTAAAGATGGGGCTCGTAAATGCATTATAGCGACAAATATTGCTGAGACATCTTTGACCGTAGATGGTATATTTTATGTCATTGACACTGGCTATGGTAAAATGAAAGTTTACAACCCTAGGATGGGTATGGATGCTCTTCAAGTATTTCCTAGTAGTCGTGCTGCTGCTGACCAACGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGAACATGTTATCGGTTGTACACAGAGAGTGCCTACCTAAATGAGATGCTGGCCAGTCCTGTACCAGAGATCCAGAGGACGAACCTTGGCTACGTGGTCTTGTTGCTGAAGTCTCTGAAGGTTGATAATTTACTTGATTTTGATTTCATGGACCCACCTCCCCAGGAAAATATTCTCAATTCTATGTACCAGCTGTGGGTCTTGGGAGCTCTCGACAATGTGGGAGGTTTGACTGCCTTAGGCTGGAAAATGGTGGAGTTCCCATTGGACCCTCCACTTGCTAAGATGTTGTTGATGGGTGAAGAGCTGGGATGCGTAGATGAAATTTTGACAATTGTATCCATGCTTTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATAGGGCAGAGGAGAGCGATGCTGCTCGGGAAAAATTTTCTGTCCCAGAGTCCGATCACCTGACATTCTATAATGTTTATCAACAGTGGAAACAACATCAGTATCGGGGGGACTGGTGCAGTGACCATTTTTTGCATGTTAAGGGGTTAGAGAAGGCTAGAGAGGTTAGGTCCCAGCTGCTAGAGATTCTGAAGACGTTGAAAATTCCACTGACATCTTGTTGGCCTGATACTGACGTTGTGAGAAAAGCTATTTGCTCTGCATACTTTCAAAATGCTGCAAAGTTTAAGGGTGTGGGGGAGTATGTTAATTGCCGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATGGGCTGCACCCCTGAGTATGTGGTTTATCATGAACAGATTCTGACAACAAAGGAGTACATGCAGTGTGTAACAGCAGTGGAGCCGCAGTGGTTGGCTGATTGGGGACCCATGTTCTTTTCTGTAAAGGAGTCGGATACATCAGTGTTGCAGAATAAGAAGAGACAGCAGGAAGAAAAAACAGCTATGGAGGAGGAAATGGACAATCTGAGAAAGGCTCAAGCACGGGCAGAGGCAGAAAACAAGCAGAGGGAGAGGGAAGATAGGTATAAACAACAGCAGAAGCAAGTCTCATTGGCGGGTTTGCACCAGGGCTCTTCTACATATCTGAGGCCCAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1088

Amino Acids

122.32

Weight (kDa)

6.86

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 395 - 547 8.3e-09 DEAD/DEAH box helicase
AAA_22 PF13401 403 - 536 3.9e-06 AAA domain
Helicase_C PF00271 593 - 722 1.2e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 787 - 812 6.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 813 - 872 2e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 930 - 1006 5.5e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 362
AccII CGCG 1 cut(s) 1509
AciI CCGC 9 cut(s) 27, 80, 381, 527, 839, 1180, 1507, 2996, 3209
AclI AACGTT 1 cut(s) 955
AclWI GGATC 4 cut(s) 702, 857, 870, 2233
AcoI YGGCCR 4 cut(s) 600, 879, 1813, 2220
AcsI RAATTY 6 cut(s) 1200, 1640, 1767, 2493, 2582, 2751
AcuI CTGAAG 3 cut(s) 2297, 2306, 2759
AfiI CCNNNNNNNGG 8 cut(s) 1323, 1536, 1682, 2086, 2087, 2365, 2412, 2550
AflIII ACRYGT 2 cut(s) 46, 2174
AgsI TTSAA 9 cut(s) 437, 1004, 1069, 1573, 1742, 1829, 2105, 2749, 2819
AhdI GACNNNNNGTC 1 cut(s) 353
AhlI ACTAGT 1 cut(s) 188
AjiI CACGTC 4 cut(s) 377, 870, 950, 1420
AjnI CCWGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
Alw21I GWGCWC 4 cut(s) 286, 1666, 2380, 2902
Alw26I GTCTC 8 cut(s) 367, 601, 666, 1367, 2003, 2286, 3067, 3205
Alw44I GTGCAC 1 cut(s) 1662
AlwI GGATC 4 cut(s) 702, 857, 870, 2233
AlwNI CAGNNNCTG 4 cut(s) 706, 1034, 1162, 2953
Ama87I CYCGRG 3 cut(s) 17, 2574, 2890
ApaLI GTGCAC 1 cut(s) 1662
ApoI RAATTY 6 cut(s) 1200, 1640, 1767, 2493, 2582, 2751
Asp700I GAANNNNTTC 2 cut(s) 2340, 2949
AspA2I CCTAGG 1 cut(s) 2081
AspS9I GGNCC 8 cut(s) 1427, 2165, 2320, 2437, 2541, 2718, 3017, 3243
AsuHPI GGTGA 9 cut(s) 16, 214, 437, 998, 1067, 1397, 1802, 2480, 2597
AsuII TTCGAA 2 cut(s) 272, 938
AvaI CYCGRG 3 cut(s) 17, 2574, 2890
AvaII GGWCC 4 cut(s) 2320, 2437, 2718, 3017
AvrII CCTAGG 1 cut(s) 2081
AxyI CCTNAGG 1 cut(s) 2407
BaeGI GKGCMC 1 cut(s) 1666
BalI TGGCCA 3 cut(s) 602, 881, 2222
BamHI GGATCC 1 cut(s) 862
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 4 cut(s) 286, 1978, 2380, 3227
BbsI GAAGAC 1 cut(s) 2747
Bbv12I GWGCWC 4 cut(s) 286, 1666, 2380, 2902
BceAI ACGGC 1 cut(s) 1315
BcgI CGANNNNNNTGC 2 cut(s) 1184, 1218
BciT130I CCWGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
BciVI GTATCC 3 cut(s) 1711, 2518, 3040
BcoDI GTCTC 8 cut(s) 367, 601, 666, 1367, 2003, 2286, 3067, 3205
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 8 cut(s) 189, 410, 809, 908, 2082, 2115, 2706, 2729
BfmI CTRYAG 3 cut(s) 672, 1193, 1746
BfuAI ACCTGC 1 cut(s) 962
BfuI GTATCC 3 cut(s) 1711, 2518, 3040
BlnI CCTAGG 1 cut(s) 2081
BmcAI AGTACT 2 cut(s) 1464, 1712
Bme1390I CCNGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
Bme18I GGWCC 4 cut(s) 2320, 2437, 2718, 3017
BmeRI GACNNNNNGTC 1 cut(s) 353
BmeT110I CYCGRG 3 cut(s) 17, 2574, 2890
BmgBI CACGTC 4 cut(s) 377, 870, 950, 1420
BmgT120I GGNCC 8 cut(s) 1427, 2165, 2320, 2437, 2541, 2718, 3017, 3243
BmrFI CCNGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
BmrI ACTGGG 2 cut(s) 2172, 2877
BmsI GCATC 7 cut(s) 559, 1066, 2086, 2205, 2471, 2557, 2862
BmuI ACTGGG 2 cut(s) 2172, 2877
BpiI GAAGAC 1 cut(s) 2747
BplI GAGNNNNNCTC 8 cut(s) 268, 300, 628, 660, 2884, 2916, 3112, 3144
BpmI CTGGAG 1 cut(s) 1770
Bpu14I TTCGAA 2 cut(s) 272, 938
BpuEI CTTGAG 1 cut(s) 3114
BsaAI YACGTR 2 cut(s) 893, 2263
BsaJI CCNNGG 6 cut(s) 303, 1322, 2081, 2254, 2331, 3220
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 4 cut(s) 784, 814, 2984, 3014
Bsc4I CCNNNNNNNGG 8 cut(s) 1323, 1536, 1682, 2086, 2087, 2365, 2412, 2550
Bse118I RCCGGY 2 cut(s) 844, 1811
Bse1I ACTGG 9 cut(s) 615, 854, 1156, 1243, 2056, 2167, 2223, 2666, 2872
Bse21I CCTNAGG 1 cut(s) 2407
Bse3DI GCAATG 1 cut(s) 1773
BseBI CCWGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
BseDI CCNNGG 6 cut(s) 303, 1322, 2081, 2254, 2331, 3220
BseGI GGATG 9 cut(s) 154, 571, 1189, 1528, 1896, 2091, 2101, 2486, 2877
BseLI CCNNNNNNNGG 8 cut(s) 1323, 1536, 1682, 2086, 2087, 2365, 2412, 2550
BseMI GCAATG 1 cut(s) 1773
BseNI ACTGG 9 cut(s) 615, 854, 1156, 1243, 2056, 2167, 2223, 2666, 2872
BseRI GAGGAG 6 cut(s) 45, 1835, 1864, 1867, 2573, 3116
BseSI GKGCMC 1 cut(s) 1666
BseYI CCCAGC 6 cut(s) 1089, 1317, 1587, 2144, 2476, 2721
BsgI GTGCAG 3 cut(s) 758, 2686, 2902
Bsh1236I CGCG 1 cut(s) 1509
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 4 cut(s) 286, 1666, 2380, 2902
BsiHKCI CYCGRG 3 cut(s) 17, 2574, 2890
BsiSI CCGG 5 cut(s) 83, 326, 845, 1812, 2863
BslFI GGGAC 5 cut(s) 70, 2576, 2672, 2704, 3030
BslI CCNNNNNNNGG 8 cut(s) 1323, 1536, 1682, 2086, 2087, 2365, 2412, 2550
BsmAI GTCTC 8 cut(s) 367, 601, 666, 1367, 2003, 2286, 3067, 3205
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 5 cut(s) 70, 2576, 2672, 2704, 3030
BsmI GAATGC 1 cut(s) 1121
BsoBI CYCGRG 3 cut(s) 17, 2574, 2890
Bsp119I TTCGAA 2 cut(s) 272, 938
Bsp1286I GDGCHC 6 cut(s) 286, 1666, 1978, 2380, 2902, 3227
Bsp1407I TGTACA 2 cut(s) 1699, 2187
Bsp143I GATC 5 cut(s) 694, 710, 862, 2238, 2602
BspACI CCGC 9 cut(s) 27, 80, 381, 527, 839, 1180, 1507, 2996, 3209
BspFNI CGCG 1 cut(s) 1509
BspHI TCATGA 3 cut(s) 787, 1806, 2941
BspMAI CTGCAG 3 cut(s) 676, 1197, 1750
BspMI ACCTGC 1 cut(s) 962
BspPI GGATC 4 cut(s) 702, 857, 870, 2233
BspQI GCTCTTC 4 cut(s) 17, 2106, 2466, 3232
BspT104I TTCGAA 2 cut(s) 272, 938
BspT107I GGYRCC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 1773
BsrFI RCCGGY 2 cut(s) 844, 1811
BsrGI TGTACA 2 cut(s) 1699, 2187
BsrI ACTGG 9 cut(s) 615, 854, 1156, 1243, 2056, 2167, 2223, 2666, 2872
BssAI RCCGGY 2 cut(s) 844, 1811
BssECI CCNNGG 6 cut(s) 303, 1322, 2081, 2254, 2331, 3220
BssMI GATC 5 cut(s) 694, 710, 862, 2238, 2602
BssT1I CCWWGG 3 cut(s) 1322, 2081, 2254
Bst2UI CCWGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
Bst4CI ACNGT 8 cut(s) 351, 494, 1036, 1055, 1294, 1438, 2023, 2634
Bst6I CTCTTC 9 cut(s) 17, 157, 248, 269, 1359, 2106, 2466, 3065, 3232
BstAPI GCANNNNNTGC 2 cut(s) 680, 1085
BstAUI TGTACA 2 cut(s) 1699, 2187
BstBAI YACGTR 2 cut(s) 893, 2263
BstBI TTCGAA 2 cut(s) 272, 938
BstC8I GCNNGC 8 cut(s) 464, 837, 969, 1780, 1932, 1944, 2151, 2220
BstF5I GGATG 9 cut(s) 154, 571, 1189, 1528, 1896, 2091, 2101, 2486, 2877
BstFNI CGCG 1 cut(s) 1509
BstKTI GATC 5 cut(s) 697, 713, 865, 2241, 2605
BstMAI GTCTC 8 cut(s) 367, 601, 666, 1367, 2003, 2286, 3067, 3205
BstMBI GATC 5 cut(s) 694, 710, 862, 2238, 2602
BstMWI GCNNNNNNNGC 7 cut(s) 680, 841, 850, 1085, 1118, 2150, 2521
BstNI CCWGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
BstNSI RCATGY 5 cut(s) 50, 466, 2178, 2687, 2974
BstSCI CCNGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
BstSFI CTRYAG 3 cut(s) 672, 1193, 1746
BstSLI GKGCMC 1 cut(s) 1666
BstUI CGCG 1 cut(s) 1509
BstV2I GAAGAC 1 cut(s) 2747
BstX2I RGATCY 2 cut(s) 862, 2238
BstXI CCANNNNNNTGG 2 cut(s) 610, 2144
BstYI RGATCY 2 cut(s) 862, 2238
Bsu36I CCTNAGG 1 cut(s) 2407
BsuI GTATCC 3 cut(s) 1711, 2518, 3040
BtgZI GCGATG 2 cut(s) 1352, 2580
BtrI CACGTC 4 cut(s) 377, 870, 950, 1420
BtsCI GGATG 9 cut(s) 154, 571, 1189, 1528, 1896, 2091, 2101, 2486, 2877
BtsI GCAGTG 6 cut(s) 1755, 2674, 2902, 2981, 2994, 3005
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 8 cut(s) 464, 837, 969, 1780, 1932, 1944, 2151, 2220
CaiI CAGNNNCTG 4 cut(s) 706, 1034, 1162, 2953
CciI TCATGA 3 cut(s) 787, 1806, 2941
Cfr10I RCCGGY 2 cut(s) 844, 1811
Cfr13I GGNCC 8 cut(s) 1427, 2165, 2320, 2437, 2541, 2718, 3017, 3243
CseI GACGC 1 cut(s) 1580
CspCI CAANNNNNGTGG 2 cut(s) 1311, 1346
DpnI GATC 5 cut(s) 696, 712, 864, 2240, 2604
DpnII GATC 5 cut(s) 694, 710, 862, 2238, 2602
DriI GACNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 4 cut(s) 600, 879, 1813, 2220
Eam1104I CTCTTC 9 cut(s) 17, 157, 248, 269, 1359, 2106, 2466, 3065, 3232
Eam1105I GACNNNNNGTC 1 cut(s) 353
EarI CTCTTC 9 cut(s) 17, 157, 248, 269, 1359, 2106, 2466, 3065, 3232
Ecl136II GAGCTC 2 cut(s) 284, 2378
Eco130I CCWWGG 3 cut(s) 1322, 2081, 2254
Eco147I AGGCCT 1 cut(s) 799
Eco24I GRGCYC 4 cut(s) 286, 1978, 2380, 3227
Eco32I GATATC 1 cut(s) 1795
Eco47I GGWCC 4 cut(s) 2320, 2437, 2718, 3017
Eco53kI GAGCTC 2 cut(s) 284, 2378
Eco57I CTGAAG 3 cut(s) 2297, 2306, 2759
Eco81I CCTNAGG 1 cut(s) 2407
Eco88I CYCGRG 3 cut(s) 17, 2574, 2890
EcoICRI GAGCTC 2 cut(s) 284, 2378
EcoO109I RGGNCCY 2 cut(s) 2718, 3017
EcoRII CCWGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
EcoRV GATATC 1 cut(s) 1795
EcoT14I CCWWGG 3 cut(s) 1322, 2081, 2254
EcoT22I ATGCAT 1 cut(s) 1988
EcoT38I GRGCYC 4 cut(s) 286, 1978, 2380, 3227
ErhI CCWWGG 3 cut(s) 1322, 2081, 2254
Esp3I CGTCTC 1 cut(s) 367
FalI AAGNNNNNCTT 6 cut(s) 762, 794, 787, 819, 1697, 1729
FaqI GGGAC 5 cut(s) 70, 2576, 2672, 2704, 3030
FauI CCCGC 3 cut(s) 374, 1173, 3202
FauNDI CATATG 1 cut(s) 1095
FblI GTMKAC 1 cut(s) 362
FokI GGATG 9 cut(s) 161, 578, 1196, 1535, 1903, 2098, 2108, 2493, 2884
FriOI GRGCYC 4 cut(s) 286, 1978, 2380, 3227
FspBI CTAG 8 cut(s) 189, 410, 809, 908, 2082, 2115, 2706, 2729
GsaI CCCAGC 6 cut(s) 1093, 1321, 1591, 2148, 2480, 2725
GsuI CTGGAG 1 cut(s) 1770
HapII CCGG 5 cut(s) 83, 326, 845, 1812, 2863
HgaI GACGC 1 cut(s) 1580
HincII GTYRAC 1 cut(s) 953
HindII GTYRAC 1 cut(s) 953
HinfI GANTC 6 cut(s) 428, 1487, 2597, 2734, 2950, 3041
HpaII CCGG 5 cut(s) 83, 326, 845, 1812, 2863
HphI GGTGA 9 cut(s) 16, 214, 437, 998, 1067, 1397, 1802, 2480, 2597
Hpy166II GTNNAC 7 cut(s) 363, 407, 953, 1297, 1664, 2074, 2190
Hpy8I GTNNAC 7 cut(s) 363, 407, 953, 1297, 1664, 2074, 2190
Hpy99I CGWCG 1 cut(s) 1596
HpyAV CCTTC 7 cut(s) 260, 415, 431, 509, 580, 2281, 2695
HpyCH4III ACNGT 8 cut(s) 351, 494, 1036, 1055, 1294, 1438, 2023, 2634
HpyF10VI GCNNNNNNNGC 7 cut(s) 680, 841, 850, 1085, 1118, 2150, 2521
KflI GGGWCCC 1 cut(s) 3017
Kzo9I GATC 5 cut(s) 694, 710, 862, 2238, 2602
LguI GCTCTTC 4 cut(s) 17, 2106, 2466, 3232
LmnI GCTCC 5 cut(s) 281, 841, 964, 2375, 2992
LweI GCATC 7 cut(s) 559, 1066, 2086, 2205, 2471, 2557, 2862
MaeI CTAG 8 cut(s) 189, 410, 809, 908, 2082, 2115, 2706, 2729
MaeIII GTNAC 9 cut(s) 488, 1188, 1286, 1420, 1539, 1612, 1750, 2669, 2980
MalI GATC 5 cut(s) 696, 712, 864, 2240, 2604
MbiI CCGCTC 1 cut(s) 27
MboI GATC 5 cut(s) 694, 710, 862, 2238, 2602
MfeI CAATTG 1 cut(s) 2502
MflI RGATCY 2 cut(s) 862, 2238
MhlI GDGCHC 6 cut(s) 286, 1666, 1978, 2380, 2902, 3227
MlsI TGGCCA 3 cut(s) 602, 881, 2222
MluNI TGGCCA 3 cut(s) 602, 881, 2222
MlyI GAGTC 2 cut(s) 2606, 3050
MmeI TCCRAC 2 cut(s) 1210, 3024
Mox20I TGGCCA 3 cut(s) 602, 881, 2222
Mph1103I ATGCAT 1 cut(s) 1988
MroXI GAANNNNTTC 2 cut(s) 2340, 2949
MscI TGGCCA 3 cut(s) 602, 881, 2222
MseI TTAA 6 cut(s) 1023, 1602, 1629, 2688, 2835, 2856
MslI CAYNNNNRTG 4 cut(s) 51, 1023, 1281, 1805
Msp20I TGGCCA 3 cut(s) 602, 881, 2222
MspA1I CMGCKG 5 cut(s) 527, 1927, 1934, 2362, 2725
MspI CCGG 5 cut(s) 83, 326, 845, 1812, 2863
MspR9I CCNGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
MunI CAATTG 1 cut(s) 2502
Mva1269I GAATGC 1 cut(s) 1121
MvaI CCWGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
MvnI CGCG 1 cut(s) 1509
MwoI GCNNNNNNNGC 7 cut(s) 680, 841, 850, 1085, 1118, 2150, 2521
NdeI CATATG 1 cut(s) 1095
NdeII GATC 5 cut(s) 694, 710, 862, 2238, 2602
NmuCI GTSAC 6 cut(s) 488, 1420, 1539, 1612, 1750, 2669
NsiI ATGCAT 1 cut(s) 1988
NspI RCATGY 5 cut(s) 50, 466, 2178, 2687, 2974
NspV TTCGAA 2 cut(s) 272, 938
PaeI GCATGC 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 3 cut(s) 787, 1806, 2941
PceI AGGCCT 1 cut(s) 799
PciI ACATGT 2 cut(s) 46, 2174
PciSI GCTCTTC 4 cut(s) 17, 2106, 2466, 3232
PcsI WCGNNNNNNNCGW 1 cut(s) 2146
PctI GAATGC 1 cut(s) 1121
PdmI GAANNNNTTC 2 cut(s) 2340, 2949
PfeI GAWTC 4 cut(s) 428, 1487, 2734, 2950
PfoI TCCNGGA 1 cut(s) 1681
PleI GAGTC 2 cut(s) 2605, 3049
PpsI GAGTC 2 cut(s) 2605, 3049
Ppu21I YACGTR 2 cut(s) 893, 2263
PpuMI RGGWCCY 2 cut(s) 2718, 3017
PscI ACATGT 2 cut(s) 46, 2174
Psp124BI GAGCTC 2 cut(s) 286, 2380
Psp1406I AACGTT 1 cut(s) 955
Psp5II RGGWCCY 2 cut(s) 2718, 3017
Psp6I CCWGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
PspFI CCCAGC 6 cut(s) 1089, 1317, 1587, 2144, 2476, 2721
PspGI CCWGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
PspPI GGNCC 8 cut(s) 1427, 2165, 2320, 2437, 2541, 2718, 3017, 3243
PspPPI RGGWCCY 2 cut(s) 2718, 3017
PsrI GAACNNNNNNTAC 2 cut(s) 2243, 2275
PstI CTGCAG 3 cut(s) 676, 1197, 1750
PstNI CAGNNNCTG 4 cut(s) 706, 1034, 1162, 2953
PsuI RGATCY 2 cut(s) 862, 2238
PvuII CAGCTG 4 cut(s) 1927, 1934, 2362, 2725
RseI CAYNNNNRTG 4 cut(s) 51, 1023, 1281, 1805
SacI GAGCTC 2 cut(s) 286, 2380
SapI GCTCTTC 4 cut(s) 17, 2106, 2466, 3232
SaqAI TTAA 6 cut(s) 1023, 1602, 1629, 2688, 2835, 2856
Sau3AI GATC 5 cut(s) 694, 710, 862, 2238, 2602
Sau96I GGNCC 8 cut(s) 1427, 2165, 2320, 2437, 2541, 2718, 3017, 3243
ScaI AGTACT 2 cut(s) 1464, 1712
SchI GAGTC 2 cut(s) 2606, 3050
ScrFI CCNGG 7 cut(s) 118, 304, 1683, 1788, 2169, 2333, 3221
SduI GDGCHC 6 cut(s) 286, 1666, 1978, 2380, 2902, 3227
SfaNI GCATC 7 cut(s) 559, 1066, 2086, 2205, 2471, 2557, 2862
SfcI CTRYAG 3 cut(s) 672, 1193, 1746
Sfr274I CTCGAG 1 cut(s) 17
SfuI TTCGAA 2 cut(s) 272, 938
SinI GGWCC 4 cut(s) 2320, 2437, 2718, 3017
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 4 cut(s) 51, 1023, 1281, 1805
SmlI CTYRAG 2 cut(s) 17, 3129
SmoI CTYRAG 2 cut(s) 17, 3129
SpeI ACTAGT 1 cut(s) 188
SphI GCATGC 1 cut(s) 466
SseBI AGGCCT 1 cut(s) 799
SsiI CCGC 9 cut(s) 27, 80, 381, 527, 839, 1180, 1507, 2996, 3209
SspI AATATT 2 cut(s) 2002, 2341
SspMI CTAG 8 cut(s) 189, 410, 809, 908, 2082, 2115, 2706, 2729
SstI GAGCTC 2 cut(s) 286, 2380
StuI AGGCCT 1 cut(s) 799
StyD4I CCNGG 7 cut(s) 116, 302, 1681, 1786, 2167, 2331, 3219
StyI CCWWGG 3 cut(s) 1322, 2081, 2254
TaaI ACNGT 8 cut(s) 351, 494, 1036, 1055, 1294, 1438, 2023, 2634
TaqI TCGA 7 cut(s) 18, 272, 371, 580, 938, 1204, 2382
TatI WGTACW 7 cut(s) 404, 1446, 1462, 1699, 1710, 2187, 2967
TauI GCSGC 2 cut(s) 82, 2998
TfiI GAWTC 4 cut(s) 428, 1487, 2734, 2950
Tru1I TTAA 6 cut(s) 1023, 1602, 1629, 2688, 2835, 2856
Tru9I TTAA 6 cut(s) 1023, 1602, 1629, 2688, 2835, 2856
TseFI GTSAC 6 cut(s) 488, 1420, 1539, 1612, 1750, 2669
Tsp45I GTSAC 6 cut(s) 488, 1420, 1539, 1612, 1750, 2669
TspGWI ACGGA 1 cut(s) 74
VneI GTGCAC 1 cut(s) 1662
VpaK11BI GGWCC 4 cut(s) 2320, 2437, 2718, 3017
XapI RAATTY 6 cut(s) 1200, 1640, 1767, 2493, 2582, 2751
XceI RCATGY 5 cut(s) 50, 466, 2178, 2687, 2974
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 17
XmaJI CCTAGG 1 cut(s) 2081
XmiI GTMKAC 1 cut(s) 362
XmnI GAANNNNTTC 2 cut(s) 2340, 2949
XspI CTAG 8 cut(s) 189, 410, 809, 908, 2082, 2115, 2706, 2729
ZrmI AGTACT 2 cut(s) 1464, 1712
Zsp2I ATGCAT 1 cut(s) 1988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.