MD16G1117100.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002001
Physical Location & Seq
Forward (+)
8307260 .. 8315417
8158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1117100.v1.1.491

Sequence Viewer

Length: 3894 bp
ATGGAGAAACGAGGTCACGCTGAACCTATTGACCTGGACAAGACAACGGCGACGTTGGAACCTGAGGCGAATAGCGATGCGGGATTATTTGTACCTGGGAAGGATAGAGTTGTTTTTAGACCTCCAGAGAGGAAATCTCTCCTGGGGTTAGATGTCCTTGCTATTGCCAAACGGGAAGGGTCTAAGGTTAACGGTGGATTCAAGACCCCAAGAGAAAGAGGTGCTAATGTTGTCTCATCAATGGAGGAAGACGAAAAATCAGAGTCTGTAATTTCAGATGAAAAAGGTACTGATGAACCCACTGCTATACGAAGTCACTCTCGGAGGCGATATCGGGAAATATCTGGGAGTGAGACACCACGCACAGAAAGTACTGTAACAGAAGAAGGAAAAATTGATGATATGCACGAGAATCGCTACTCTAGGGAACATCTGCGTGGAGATGTTTCGTCTCCTAGTGGAAGTTCCCACAGTGTCAGGTCTCGGACCCCTAAGTATGACAGGGATGATCGTGGAAATGAGAGAAGAGATTACAAGGGTAGATACGACAGGGATGACCGTGGTAGTGAGAGAAGAGAGTATCAGGATGGTAGTAGGAGTGAAAGACAAAGATACAGTAGTAATGGTAACGATTATAATCGTCGAAGGGAAGGTAGCCGATATGAACAAGAATATAGTGGAGAATATGGAAGAAAGCGGAGTAGATATGAAGATTCAAAGAGGACACCTGGCAGATCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGTCACCACGACGTGATAACTACCCTAATACCAGTAGGCGACATCAACCATCACCGGCACCTATGCTGCTTGGTGCCTCACCTGATGCACGACTAGTTTCCCCATGGTTAGGTGGCCATACACCTGGTTCTGCTGCTTCTCCTTGGGATCATGTCTCTCCCTCTCCAGCTCCAATACGTGCTTCTGGATACTCAGTCAAATCTTCAAGTTCGAAATATGGTGCGAGGTCCCATCAACTTCCTTTTTCTTCAGAAAGTTCACAATCATTCAAGGATGGGGAAGAAGATAATTTGGCCGAAGAACACACATACGAGATTACTGAAAGCATGCGTCAAGAGATGGAATACAATTCTGATCGTGCATGGTATGATAGAGAAGAAGGAAACACAATGTTTGATACGACTGATGACTCGTCCTTGTTTTATGCAAACGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCGAAAAGGCTGGTCCGAAAAGATGGAACTAAGATGTCTCTTGCTCAGAGCAAAAAGTTGTCTCAACTCACTGCTGATAATGCTCAGTGGGAGGATCGTCAACTTTTAAGATCTGGTGCTGTTAGAGGTACCGAGGTGCAGACTGAGTTTGATGATGAAGAAGAACGGAGAGTCATTCTTCTTGTCCATGATACAAAGCCTCCTTTCCTGGATGGCAGGGTTGTTTTTACCAAACAAGCAGAGCCCATAATGCCTATAAAGGATCCCACATCGGATATGGCCATAATTTCACGCAAAGGGTCTGCTCTGGTTAGGGAAATCCATGAGAAGCAAAGTCAGAACAAATCACGTCAACGTTTCTGGGAGCTTGCAGGTTCCAAACTGGGTGATATCCTTGGTGTTGAAAAAACGGCGGAGCAGATTGATGCGGATACTGCTGCAGTAGGTGAAGATGGTGAAATTGATTTCAAAGAAGATGCGAAGTTTGCACAACATATGAAGAGCGGGGAGGCTGTGAGTGATTTTGCATTGTCAAAAACACTCTCACAGCAACGACAATATTTGCCCATATTTTCTGTGCGGGATGAGTTATTGCAGGTAATTCGAGAAAATCAGGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAATTGACACAGTATCTTTATGAAGATGGATACACAGTAAATGGTATAGTGGGTTGCACCCAACCAAGGCGTGTAGCAGCCATGAGTGTTGCAAAAAGAGTAAGTGAAGAGATGGAGACTGAGCTTGGTGATAAAGTTGGCTATGCTATTCGTTTTGAGGATGTGACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTACTTCTGCGCGAAACACTCAGAGATTCTGATCTTGACAAATACCGTGTAGTTGTCATGGACGAAGCCCATGAAAGGTCACTAAACACAGATGTGCTGTTTGGAATTTTGAAAAAAGTGGTGGCCCAACGTCGTGATTTTAAGCTCATTGTGACATCTGCAACTCTAAATGCTCAGAAATTTTCAAATTTCTTTGGGAGTGTACCAATTTTTCACATTCCTGGGAGAACGTTTCCTGTAAATATCTTGTTCAGCAAAACCCCTTGTGAAGATTATGTTGAAGGTGCAGTGAAGCAGGCCATGACAATTCACATCACGAGCCCTCCCGGTGATATCCTCATCTTCATGACAGGCCAAGATGAGATTGAGGCAGCTTGTTATGCGCTTGCCGAGCGGATGGAACAACTTATCTCGTCCTCCAAAAAAGCAGTTCCAAAACTCTTGATACTCCCTATATATTCACAGTTGCCTGCTGATTTGCAAGCAAAGATATTTCAAAAAGCTGAGGATGGAGCTCGTAAATGCATTGTAGCAACCAACATTGCTGAGACTTCTTTGACTGTGGATGGAATATATTACGTGATCGATACGGGCTATGGTAAGATGAAGGTATACAATCCTCGAATGGGTATGGATGCTCTTCAAGTGTTTCCTGTTAGTCGTGCAGCTGCTGACCAGCGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGGACATGTTATCGGCTGTACACGGAGAATGCATATCTAAATGAATTGCTGCCTAGTCCTGTGCCAGAGATTCAGAGGACTAACCTTGGTAATGTGGTGTTGTTGCTCAAGAGTCTTAAGGTTGAGAACTTGTTAGACTTTGATTTCATGGACCCACCTCCACAGGACAATATTCTCAACTCTATGTACCAATTGTGGGTCTTAGGTGCTCTGAACAATGTCGGAGGCTTAACTGAACTAGGCTGGAAGATGGTGGAGTTCCCATTGGACCCCCCGCTTGCTAAGATGTTATTGATGGGCGATCAGCTTGGATGCCTGGATGAGGTTCTGACAATTGTGTCTATGCTTTCGGTGCCATCAGTGTTCTTTAGGCCTAAAGATAGGGCAGAGGAGAGCGATGCTGCAAGGGAGAAGTTCTCTGTTCCAGAATCTGACCACTTGACGCTGTATAATGTGTACCAGCAGTGGAAACAACATCAGTATCGGGGGGACTGGTGTGGAGATCATTTTTTGCATGTTAAAGGCTTACGAAAGGCTAGAGAGGTGAGGTCCCAACTGCTGGAGATCCTGAAGACGCTAAAAATTCCTCTAACATCCTGTTGGCCCGATACTGATCTTGTGAGAAAAGCTATATGCTCCTCGTACTTCCACAATTCTGCAAGATTGAAGGGAGTTGGGGAGTATGTGAACTGCAGGACCGGGATGCCATGCCATTTACATCCGAGCAGTGCTCTTTACGGTATGGGGTGCACTCCAGATTATGTGGTTTATCATGAACTGATTTTGACGGCAAAGGAGTACATGCAGTGCGCCACAGCGGTGGAGCCGCAGTGGTTGGCCGAGTTGGGGCCCATGTTCTTTTCTGTGAAGGATTCAGATACATCGATGCTGGAGCATAAGAAGAGACAAAAGGAGGAGAAAACAGCGATGGAGGAGGAAATGGAGAACCTGAGGAAAACTCAAGCGGAGGAAGAGAAAGAAAGCAAGCGGAAGGAGAGGGAAAAGAGATCAAAGCAGCAGCAGCAAGTCTCGATGCCAGGTTTGCACAAGGGATCTTCTACATATTTGAGGCCAAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1298

Amino Acids

146.8

Weight (kDa)

6.03

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 607 - 757 9.8e-09 DEAD/DEAH box helicase
Helicase_C PF00271 804 - 933 4.3e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 998 - 1023 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1024 - 1083 4.3e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1141 - 1218 7.6e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AasI GACNNNNNNGTC 1 cut(s) 3139
Acc36I ACCTGC 2 cut(s) 1595, 1819
Acc65I GGTACC 1 cut(s) 1361
AccB1I GGYRCC 4 cut(s) 826, 842, 1361, 3154
AccB7I CCANNNNNTGG 1 cut(s) 3361
AccBSI CCGCTC 2 cut(s) 1737, 2488
AccI GTMKAC 1 cut(s) 2706
AccII CGCG 1 cut(s) 2106
AclI AACGTT 2 cut(s) 1588, 2324
AclWI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
AcoI YGGCCR 4 cut(s) 883, 1062, 1512, 3639
AcsI RAATTY 4 cut(s) 2199, 2273, 2281, 3384
AcuI CTGAAG 2 cut(s) 1002, 3392
AdeI CACNNNGTG 1 cut(s) 782
AfiI CCNNNNNNNGG 8 cut(s) 878, 1956, 2169, 2720, 2998, 3124, 3361, 3648
AflII CTTAAG 1 cut(s) 2918
AflIII ACRYGT 1 cut(s) 2807
AhlI ACTAGT 1 cut(s) 862
AjiI CACGTC 2 cut(s) 782, 1583
AjuI GAANNNNNNNTTGG 2 cut(s) 934, 966
AleI CACNNNNGTG 2 cut(s) 2186, 3620
Alw21I GWGCWC 4 cut(s) 2611, 3013, 3535, 3554
Alw44I GTGCAC 1 cut(s) 3550
AlwI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
AlwNI CAGNNNCTG 3 cut(s) 266, 2765, 3233
ApaI GGGCCC 1 cut(s) 3654
ApaLI GTGCAC 1 cut(s) 3550
ApoI RAATTY 4 cut(s) 2199, 2273, 2281, 3384
Asp718I GGTACC 1 cut(s) 1361
AspLEI GCGC 3 cut(s) 2106, 2479, 3614
AsuC2I CCSGG 2 cut(s) 2421, 3502
AsuHPI GGTGA 9 cut(s) 765, 813, 840, 1631, 1691, 1700, 2030, 2435, 3358
AsuII TTCGAA 1 cut(s) 980
AvaII GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
AxyI CCTNAGG 2 cut(s) 63, 3752
BaeGI GKGCMC 2 cut(s) 3554, 3654
BaeI ACNNNNGTAYC 6 cut(s) 604, 637, 1353, 1386, 3266, 3299
BalI TGGCCA 2 cut(s) 885, 1514
BamHI GGATCC 1 cut(s) 1495
BanI GGYRCC 4 cut(s) 826, 842, 1361, 3154
BanII GRGCYC 4 cut(s) 1479, 2417, 2611, 3654
BauI CACGAG 2 cut(s) 407, 2410
BbsI GAAGAC 2 cut(s) 255, 3380
Bbv12I GWGCWC 4 cut(s) 2611, 3013, 3535, 3554
BbvCI CCTCAGC 1 cut(s) 2598
BceAI ACGGC 3 cut(s) 63, 1659, 3606
BcgI CGANNNNNNTGC 4 cut(s) 395, 429, 1817, 1851
BciVI GTATCC 3 cut(s) 950, 1657, 1913
BcnI CCSGG 2 cut(s) 2421, 3502
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 6 cut(s) 423, 456, 863, 2856, 3041, 3339
BfmI CTRYAG 2 cut(s) 1671, 3493
BfrI CTTAAG 1 cut(s) 2918
BfuAI ACCTGC 2 cut(s) 1595, 1819
BfuI GTATCC 3 cut(s) 950, 1657, 1913
BglII AGATCT 2 cut(s) 734, 1343
BmcAI AGTACT 2 cut(s) 373, 2097
Bme18I GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
BmgBI CACGTC 2 cut(s) 782, 1583
BmrI ACTGGG 4 cut(s) 751, 1625, 2067, 2805
BmuI ACTGGG 4 cut(s) 751, 1625, 2067, 2805
BpiI GAAGAC 2 cut(s) 255, 3380
BplI GAGNNNNNCTC 8 cut(s) 121, 153, 3203, 3235, 3517, 3549, 3745, 3777
BpmI CTGGAG 5 cut(s) 108, 918, 3383, 3540, 3713
Bpu10I CCTNAGC 1 cut(s) 2598
Bpu14I TTCGAA 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 2894, 3747
BpuMI CCSGG 2 cut(s) 2421, 3502
Bsa29I ATCGAT 2 cut(s) 2679, 3686
BsaAI YACGTR 2 cut(s) 947, 2674
BsaBI GATNNNNATC 3 cut(s) 636, 2124, 3414
BsaI GGTCTC 1 cut(s) 486
BsaXI ACNNNNNCTCC 6 cut(s) 1417, 1447, 2401, 2431, 3617, 3647
Bsc4I CCNNNNNNNGG 8 cut(s) 878, 1956, 2169, 2720, 2998, 3124, 3361, 3648
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 7 cut(s) 746, 801, 1620, 1876, 2062, 2800, 3299
Bse21I CCTNAGG 2 cut(s) 63, 3752
Bse3DI GCAATG 1 cut(s) 2634
Bse8I GATNNNNATC 3 cut(s) 636, 2124, 3414
BseCI ATCGAT 2 cut(s) 2679, 3686
BseJI GATNNNNATC 3 cut(s) 636, 2124, 3414
BseLI CCNNNNNNNGG 8 cut(s) 878, 1956, 2169, 2720, 2998, 3124, 3361, 3648
BseMI GCAATG 1 cut(s) 2634
BseNI ACTGG 7 cut(s) 746, 801, 1620, 1876, 2062, 2800, 3299
BseRI GAGGAG 5 cut(s) 782, 3206, 3430, 3731, 3749
BseSI GKGCMC 2 cut(s) 3554, 3654
BseYI CCCAGC 1 cut(s) 2777
BsgI GTGCAG 3 cut(s) 1391, 2400, 2778
Bsh1236I CGCG 1 cut(s) 2106
BshNI GGYRCC 4 cut(s) 826, 842, 1361, 3154
BshVI ATCGAT 2 cut(s) 2679, 3686
BsiHKAI GWGCWC 4 cut(s) 2611, 3013, 3535, 3554
BsiSI CCGG 3 cut(s) 824, 2421, 3501
BslFI GGGAC 4 cut(s) 982, 2818, 3305, 3337
BslI CCNNNNNNNGG 8 cut(s) 878, 1956, 2169, 2720, 2998, 3124, 3361, 3648
BsmBI CGTCTC 1 cut(s) 456
BsmFI GGGAC 4 cut(s) 982, 2818, 3305, 3337
BsmI GAATGC 1 cut(s) 2836
Bso31I GGTCTC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 980
Bsp120I GGGCCC 1 cut(s) 3650
Bsp1286I GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
Bsp1407I TGTACA 1 cut(s) 2820
Bsp19I CCATGG 1 cut(s) 872
BspDI ATCGAT 2 cut(s) 2679, 3686
BspFNI CGCG 1 cut(s) 2106
BspHI TCATGA 2 cut(s) 2439, 3574
BspMAI CTGCAG 2 cut(s) 1675, 3497
BspMI ACCTGC 2 cut(s) 1595, 1819
BspPI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
BspQI GCTCTTC 2 cut(s) 1727, 2739
BspT104I TTCGAA 1 cut(s) 980
BspT107I GGYRCC 4 cut(s) 826, 842, 1361, 3154
BspTI CTTAAG 1 cut(s) 2918
BspTNI GGTCTC 1 cut(s) 486
BsrBI CCGCTC 2 cut(s) 1737, 2488
BsrDI GCAATG 1 cut(s) 2634
BsrFI RCCGGY 1 cut(s) 823
BsrGI TGTACA 1 cut(s) 2820
BsrI ACTGG 7 cut(s) 746, 801, 1620, 1876, 2062, 2800, 3299
BssAI RCCGGY 1 cut(s) 823
BssNAI GTATAC 1 cut(s) 2707
BssSI CACGAG 2 cut(s) 407, 2410
BssT1I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Bst1107I GTATAC 1 cut(s) 2707
Bst2BI CACGAG 2 cut(s) 407, 2410
Bst6I CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
BstAFI CTTAAG 1 cut(s) 2918
BstAPI GCANNNNNTGC 1 cut(s) 1202
BstAUI TGTACA 1 cut(s) 2820
BstBAI YACGTR 2 cut(s) 947, 2674
BstBI TTCGAA 1 cut(s) 980
BstDSI CCRYGG 2 cut(s) 559, 872
BstENI CCTNNNNNAGG 1 cut(s) 3122
BstFNI CGCG 1 cut(s) 2106
BstHHI GCGC 3 cut(s) 2106, 2479, 3614
BstNSI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
BstSFI CTRYAG 2 cut(s) 1671, 3493
BstSLI GKGCMC 2 cut(s) 3554, 3654
BstUI CGCG 1 cut(s) 2106
BstV2I GAAGAC 2 cut(s) 255, 3380
BstX2I RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
BstXI CCANNNNNNTGG 3 cut(s) 893, 2777, 3622
BstYI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
BstZ17I GTATAC 1 cut(s) 2707
Bsu15I ATCGAT 2 cut(s) 2679, 3686
Bsu36I CCTNAGG 2 cut(s) 63, 3752
BsuI GTATCC 3 cut(s) 950, 1657, 1913
BsuTUI ATCGAT 2 cut(s) 2679, 3686
BtgI CCRYGG 2 cut(s) 559, 872
BtgZI GCGATG 3 cut(s) 90, 3213, 3743
BtrI CACGTC 2 cut(s) 782, 1583
BtsI GCAGTG 7 cut(s) 300, 1302, 2388, 3272, 3535, 3614, 3638
BveI ACCTGC 2 cut(s) 1595, 1819
CaiI CAGNNNCTG 3 cut(s) 266, 2765, 3233
CciI TCATGA 2 cut(s) 2439, 3574
CfoI GCGC 3 cut(s) 2106, 2479, 3614
Cfr10I RCCGGY 1 cut(s) 823
ClaI ATCGAT 2 cut(s) 2679, 3686
CseI GACGC 3 cut(s) 1088, 3253, 3385
CsiI ACCWGGT 1 cut(s) 892
DraIII CACNNNGTG 1 cut(s) 782
DrdI GACNNNNNNGTC 1 cut(s) 3139
DseDI GACNNNNNNGTC 1 cut(s) 3139
EaeI YGGCCR 4 cut(s) 883, 1062, 1512, 3639
Eam1104I CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
EarI CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
EciI GGCGGA 1 cut(s) 1661
Ecl136II GAGCTC 1 cut(s) 2609
Eco130I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Eco147I AGGCCT 1 cut(s) 3175
Eco24I GRGCYC 4 cut(s) 1479, 2417, 2611, 3654
Eco31I GGTCTC 1 cut(s) 486
Eco32I GATATC 3 cut(s) 332, 1624, 2428
Eco47I GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
Eco53kI GAGCTC 1 cut(s) 2609
Eco57I CTGAAG 2 cut(s) 1002, 3392
Eco81I CCTNAGG 2 cut(s) 63, 3752
EcoICRI GAGCTC 1 cut(s) 2609
EcoNI CCTNNNNNAGG 1 cut(s) 3122
EcoO109I RGGNCCY 3 cut(s) 996, 3351, 3650
EcoRV GATATC 3 cut(s) 332, 1624, 2428
EcoT14I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
EcoT22I ATGCAT 2 cut(s) 2621, 2836
EcoT38I GRGCYC 4 cut(s) 1479, 2417, 2611, 3654
ErhI CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Esp3I CGTCTC 1 cut(s) 456
FaqI GGGAC 4 cut(s) 982, 2818, 3305, 3337
FauI CCCGC 4 cut(s) 73, 1730, 1806, 3084
FauNDI CATATG 1 cut(s) 1728
FblI GTMKAC 1 cut(s) 2706
FriOI GRGCYC 4 cut(s) 1479, 2417, 2611, 3654
FspBI CTAG 6 cut(s) 423, 456, 863, 2856, 3041, 3339
GlaI GCGC 3 cut(s) 2105, 2478, 3613
GsaI CCCAGC 1 cut(s) 2781
GsuI CTGGAG 5 cut(s) 108, 918, 3383, 3540, 3713
HapII CCGG 3 cut(s) 824, 2421, 3501
HgaI GACGC 3 cut(s) 1088, 3253, 3385
HhaI GCGC 3 cut(s) 2106, 2479, 3614
Hin6I GCGC 3 cut(s) 2104, 2477, 3612
HinP1I GCGC 3 cut(s) 2104, 2477, 3612
HincII GTYRAC 3 cut(s) 190, 1334, 1586
HindII GTYRAC 3 cut(s) 190, 1334, 1586
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 3 cut(s) 824, 2421, 3501
HphI GGTGA 9 cut(s) 765, 813, 840, 1631, 1691, 1700, 2030, 2435, 3358
Hpy99I CGWCG 4 cut(s) 55, 645, 783, 2229
HpyCH4IV ACGT 8 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2673
HpySE526I ACGT 8 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2673
HspAI GCGC 3 cut(s) 2104, 2477, 3612
KpnI GGTACC 1 cut(s) 1365
KspAI GTTAAC 1 cut(s) 190
LguI GCTCTTC 2 cut(s) 1727, 2739
LmnI GCTCC 7 cut(s) 943, 1597, 1648, 2606, 3443, 3625, 3694
MabI ACCWGGT 1 cut(s) 892
MaeI CTAG 6 cut(s) 423, 456, 863, 2856, 3041, 3339
MaeII ACGT 8 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2673
MaeIII GTNAC 8 cut(s) 14, 314, 376, 626, 771, 2053, 2172, 2245
MbiI CCGCTC 2 cut(s) 1737, 2488
MfeI CAATTG 3 cut(s) 1892, 2993, 3135
MflI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
MhlI GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
MlsI TGGCCA 2 cut(s) 885, 1514
MluNI TGGCCA 2 cut(s) 885, 1514
MlyI GAGTC 5 cut(s) 272, 779, 1172, 1413, 2923
MmeI TCCRAC 3 cut(s) 36, 1843, 3004
Mox20I TGGCCA 2 cut(s) 885, 1514
Mph1103I ATGCAT 2 cut(s) 2621, 2836
MscI TGGCCA 2 cut(s) 885, 1514
MseI TTAA 6 cut(s) 189, 1340, 2235, 2919, 3032, 3321
MslI CAYNNNNRTG 4 cut(s) 435, 2186, 2438, 3620
Msp20I TGGCCA 2 cut(s) 885, 1514
MspA1I CMGCKG 2 cut(s) 2762, 3620
MspCI CTTAAG 1 cut(s) 2918
MspI CCGG 3 cut(s) 824, 2421, 3501
MunI CAATTG 3 cut(s) 1892, 2993, 3135
Mva1269I GAATGC 1 cut(s) 2836
MvnI CGCG 1 cut(s) 2106
NciI CCSGG 2 cut(s) 2421, 3502
NcoI CCATGG 1 cut(s) 872
NdeI CATATG 1 cut(s) 1728
NmeAIII GCCGAG 2 cut(s) 2509, 3667
NmuCI GTSAC 6 cut(s) 14, 314, 771, 2053, 2172, 2245
NsiI ATGCAT 2 cut(s) 2621, 2836
NspI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
NspV TTCGAA 1 cut(s) 980
OliI CACNNNNGTG 2 cut(s) 2186, 3620
PaeI GCATGC 1 cut(s) 1099
PagI TCATGA 2 cut(s) 2439, 3574
PceI AGGCCT 1 cut(s) 3175
PciI ACATGT 1 cut(s) 2807
PciSI GCTCTTC 2 cut(s) 1727, 2739
PctI GAATGC 1 cut(s) 2836
PfeI GAWTC 7 cut(s) 198, 412, 713, 2120, 2872, 3230, 3674
PflMI CCANNNNNTGG 1 cut(s) 3361
PfoI TCCNGGA 1 cut(s) 1440
PleI GAGTC 5 cut(s) 271, 778, 1172, 1412, 2922
PpsI GAGTC 5 cut(s) 271, 778, 1172, 1412, 2922
Ppu21I YACGTR 2 cut(s) 947, 2674
PpuMI RGGWCCY 2 cut(s) 996, 3351
PscI ACATGT 1 cut(s) 2807
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 2611
Psp1406I AACGTT 2 cut(s) 1588, 2324
Psp5II RGGWCCY 2 cut(s) 996, 3351
PspFI CCCAGC 1 cut(s) 2777
PspOMI GGGCCC 1 cut(s) 3650
PspPPI RGGWCCY 2 cut(s) 996, 3351
PstI CTGCAG 2 cut(s) 1675, 3497
PstNI CAGNNNCTG 3 cut(s) 266, 2765, 3233
PsuI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
PvuII CAGCTG 1 cut(s) 2762
RseI CAYNNNNRTG 4 cut(s) 435, 2186, 2438, 3620
SacI GAGCTC 1 cut(s) 2611
SapI GCTCTTC 2 cut(s) 1727, 2739
SaqAI TTAA 6 cut(s) 189, 1340, 2235, 2919, 3032, 3321
ScaI AGTACT 2 cut(s) 373, 2097
SchI GAGTC 5 cut(s) 272, 779, 1172, 1413, 2923
SduI GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
SexAI ACCWGGT 1 cut(s) 892
SfcI CTRYAG 2 cut(s) 1671, 3493
SfuI TTCGAA 1 cut(s) 980
SinI GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
SmiMI CAYNNNNRTG 4 cut(s) 435, 2186, 2438, 3620
SmlI CTYRAG 3 cut(s) 2909, 2918, 3762
SmoI CTYRAG 3 cut(s) 2909, 2918, 3762
SpeI ACTAGT 1 cut(s) 862
SphI GCATGC 1 cut(s) 1099
SseBI AGGCCT 1 cut(s) 3175
SspI AATATT 2 cut(s) 1793, 2974
SspMI CTAG 6 cut(s) 423, 456, 863, 2856, 3041, 3339
SstI GAGCTC 1 cut(s) 2611
StuI AGGCCT 1 cut(s) 3175
StyI CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
TaiI ACGT 8 cut(s) 56, 784, 949, 1585, 1591, 2227, 2327, 2676
TaqI TCGA 7 cut(s) 643, 980, 1837, 2679, 2716, 3686, 3833
TatI WGTACW 5 cut(s) 371, 2079, 2095, 2820, 3600
TauI GCSGC 1 cut(s) 3631
TfiI GAWTC 7 cut(s) 198, 412, 713, 2120, 2872, 3230, 3674
Tru1I TTAA 6 cut(s) 189, 1340, 2235, 2919, 3032, 3321
Tru9I TTAA 6 cut(s) 189, 1340, 2235, 2919, 3032, 3321
TseFI GTSAC 6 cut(s) 14, 314, 771, 2053, 2172, 2245
Tsp45I GTSAC 6 cut(s) 14, 314, 771, 2053, 2172, 2245
TspGWI ACGGA 2 cut(s) 1414, 2840
Van91I CCANNNNNTGG 1 cut(s) 3361
Vha464I CTTAAG 1 cut(s) 2918
VneI GTGCAC 1 cut(s) 3550
VpaK11BI GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
XagI CCTNNNNNAGG 1 cut(s) 3122
XapI RAATTY 4 cut(s) 2199, 2273, 2281, 3384
XceI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
XcmI CCANNNNNNNNNTGG 1 cut(s) 1507
XmiI GTMKAC 1 cut(s) 2706
XspI CTAG 6 cut(s) 423, 456, 863, 2856, 3041, 3339
ZrmI AGTACT 2 cut(s) 373, 2097
Zsp2I ATGCAT 2 cut(s) 2621, 2836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.