Prupe.4G121400_v2.0.a1

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0002001
Physical Location & Seq
Forward (+)
6574172 .. 6578717
4546 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G121400.2

Sequence Viewer

Length: 3408 bp
ATGGACAAGACGAAGGTAACAATGGAACCCAAGAAGAGTGGTGGTGCTGGGGCATTTTATGTTCCTGGGAATGATAGAGTTTTTTATAAGCCTCCTGAGAAGAAATCTCCTTTGGGTTTACATGTCTTTGCTAATGCTAATGCAAAACGGGAAGGCCATATAAGTGAGAGAATAGATAAGCAACATGATAGTAGTGATTATGGTAAGGATTATTATCATCAAAGGAAATCCGGTGGACGATATGAACAAGACCAATGTGGCGGAGGATATGGAAGAAAGCGGAGTAGATGTGAAGGTTCAATGAGGAAACATGACAGATCATCTGATTGGGATGATGGGAGATTGGGATGGCAAGATACCCCGCAGCATTCTAGTTACTCTAATGCTAGTAAGCGCCATCAAGCCGGTTCTGCTGCTTCTCCATGGGATTTCTCGCCAGTTGAAATTCCTTCTGGATACTCAATCAAATCTTCAAGTTCGAAACATGGCGCAGGTTCCCATAAACTTACCATTTATTCAGAAAATTCAGAGTCATTCAAGGACGGAGAAGGTGATAAGACTGATTTGGATGAAGAACACAAATACGAAATTAGAGAAATCATGCGTCAAGAGATAGATTACAATTCTGACGTTGCATGGTATGATAGAGAAGAAGGAAATGCAATGTTTGATACAACTGATAGCTCATCTTTGTTTTATGGAAATGATGCTTATTTTCAGAAGAAGGAAGCAGAGTTGGCAAAAAGATTGCTTCGAAGAGATGGCACTAAGATGAATGTTGCTCGGAGCAGAAAAATGTCTCAGCTCAATGCTGATAATGCTCGGTGGGAAGATAGTCAACTTTCACGATCTGGTGCTGTTGGTGCGACTAAGGCACAGACTGAGATTGATGATGAAGAAGAACACAAAGTTACTCTTCTTGTGCATGATACAAATTTAAAGCCTCCTTTCCTGAATGACAGTGCTGTTTTTACCAAGCAAACAGAGCCAGTAATGCCAATAAAGGATCCCACGTCTGATATGGCTATAATTTCACGCAAAGGGTCTGCTCTAGTTAGAAGAATCCATGAGAAGCAAAATTTGAATAAATCTCGTCAACGGTTTTGGGAGCTTGCAGGTTCCAAACTAGGTGAAATCCTTGGTGTTGAGAAAACAGCAGAGCAGATTGGCACAGATACTGCTGCCACAGGTACAGATGGTGAAATTGATTTCAAAGAAGATGCAAAGTTTAAGCAGCATATGAAGAATGAGGAGGCTGTGAGTGAATTTGCAAAGTCAAAAACCATCTCACAGCAACGGCAGTGTTTGCCCATATATTCTGTGAGAGAGGAATTACTACAGGTAATTCGAGAAAATCAAGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAACTGACACAATATCTGCATGAAGATGGATACACCGTAAATGGTATAGTAGCTTGCACCCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAAGAGATGGAGACTGAGCTTGGCGAGAAAGTCGGTTATACTATTCGTTTTGAGGATGTGACTGGACCGAATACCATAATTAAGTACATGACTGATGGAGTACTTATGCATGAAACACTTAGAGATTCTGATCTTGACAAGTATCGCGTAGTTGTGATGGACGAAGCCCATGAAAGGTCACTAAACACAGATGTGCTTCTTGGGATTTTGAAAAAAGTGGTTGCCCAACGTCGTGATTTTAAGCTCATTGTGACATCTGCGACACTAGATGCTCAGAAATTCCAGACTTTTTTTGGAAGTGCACCGATTTTTGAAATTCCTGGAAGAACATTTCCTGTAACTATCAACTACAATGAAACCCCATGTGAAGATTATGTTGAAGCTGCAGTGAAGAAGGCCATAACTATTCACATCACCAGCACTCACGGTGATATCCTGATCTTCATGACCGGCCAAGATGAGATTGAGGCAGCTTGTTATGCCCTCTCAGAGCGGATGGAACAACTTATCTCCACCTCGAAGAAACGGGTGCCAAAACTCTTGATACTCCCTATATATTCACAGTTGCCTGCTGACTTGCAAGCAAAGATATTCCAAAAAGCTGAAGATGGGGTTCGTAAATGCATTGTAGCAACCAACATTGCTGAGACATCTTTGACTGTAGATGGAATCCTGTATGTCATTGACACGGGTTATTGTAAAATGAAAGTATACAACCCCAAAATGTGTATGGATGCTCTTCAAGTATTTCCTATTAGTCAGACTGCTGCCAACCAACGTGCTGGGCGTGCTGGCAGAACTGAGCCTGGAACATGTTATCGGCTGTACACAAAGAATGCATACCGAAATGAAATGCTGCCTAATCCTGTGCCAGAGATTCAGAGGACCAACCTTGGGTATGTGATCTTGTTGCTCAAATCTCTTCAGGTTGATAACTTGCTAGATTTTGATTTCATGGACCCACCTCCCAAGGATAATATTCTCAATTCTATGTACCAGTTGTGGGTGTTGGGTGCTCTTAACAATCAGGGCCGCTTAACTGATCTGGGCTGGAAAATGGTACAGTTGCCATTGGACCCTCCACTTGCTAAGATGCTGTTGATGGGTGAAGAGATGGAATGCTTAGATGAGGTTTTGACGATTGTGTCCATGCTTTCGGTGCCGTCAGTATTTTTTAGGCCCAAAGATAGGGCAGAGGTGAGCGATGCTGCACGGGAGAAATTTTCCATCCCAGAGTCCGACCACTTAACACTGTATAATGTTTATCAGCAGTGGAAACAACAGCAGTATCGGGGAGACTGGTGCAATGACCATTTTTTGCATGTTAAAGGGTTGAGAAAGGCTAGAGAGGTGAGGACCCAGCTCCTAGAGATCCTGAAGACACTAAAAATCCCAATTTCATCCTGTGGGCCTGATACTGATATTGTGAAAAAAGCTATTTGCTCCGCATACTTTCACAATGCAGCAAGATTGAAGGGTGTGGTGGAGTATGTAAACTGCAGGACTGGGATGCCATGCCATTTACACCCGAGCAGTGCTCTTTATGGTATGGGATGCACTCCAGAGTATGTGGTTTATCATGAATTGATTTTGACAACAAAAGAGTATATGCAGTGTGCCACAGCAGTGGAGCCACAATGGTTGGCTGAGTTGGGACCCATGTTCTTCTATGTAAAGGAGTCAGATACGTCAATGTTGGAGCATAAGAAGACACGAAAGGAGGAGAAAACAGCTATGGAGGAGGAAATGGAGAATTTGAGAGAGGCTCAAGCAGAGGCAGAGAAAGAAAGCGAGCTGGAGAGGGAAAAGAGATCCAAGCAGCAACAGCAGCAGCGAATGTCAATGCCGGGTTTGCACCATGGCTCTTCTGCATATACGAGGCCAAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1136

Amino Acids

128.57

Weight (kDa)

6.35

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
AasI GACNNNNNNGTC 1 cut(s) 2650
Acc36I ACCTGC 2 cut(s) 482, 1106
AccB1I GGYRCC 2 cut(s) 2035, 2665
AccBSI CCGCTC 1 cut(s) 1999
AccI GTMKAC 1 cut(s) 2217
AccII CGCG 1 cut(s) 1653
AciI CCGC 6 cut(s) 261, 280, 362, 1999, 2539, 2952
AclWI GGATC 4 cut(s) 1001, 1014, 2872, 3312
AcoI YGGCCR 1 cut(s) 1957
AcsI RAATTY 9 cut(s) 444, 523, 934, 1078, 1265, 1784, 1821, 2726, 3259
AcuI CTGAAG 3 cut(s) 2130, 2414, 2903
AfaI GTAC 6 cut(s) 1192, 1592, 1608, 2333, 2501, 2568
AfiI CCNNNNNNNGG 5 cut(s) 1467, 1680, 2400, 2509, 2694
AflIII ACRYGT 2 cut(s) 121, 2318
AjiI CACGTC 1 cut(s) 1014
AjnI CCWGG 3 cut(s) 64, 1825, 2311
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AleI CACNNNNGTG 2 cut(s) 1697, 3131
Alw21I GWGCWC 3 cut(s) 1810, 2524, 3046
Alw26I GTCTC 4 cut(s) 804, 1511, 2147, 2796
Alw44I GTGCAC 1 cut(s) 1806
AlwI GGATC 4 cut(s) 1001, 1014, 2872, 3312
AlwNI CAGNNNCTG 1 cut(s) 1178
Ama87I CYCGRG 1 cut(s) 3034
AoxI GGCC 7 cut(s) 154, 1902, 1957, 2536, 2684, 2915, 3386
ApaLI GTGCAC 1 cut(s) 1806
ApoI RAATTY 9 cut(s) 444, 523, 934, 1078, 1265, 1784, 1821, 2726, 3259
AspLEI GCGC 2 cut(s) 396, 491
AspS9I GGNCC 9 cut(s) 1571, 2391, 2464, 2536, 2581, 2685, 2862, 2915, 3161
AsuC2I CCSGG 1 cut(s) 3354
AsuHPI GGTGA 8 cut(s) 563, 1142, 1211, 1912, 1946, 2624, 2716, 2869
AsuII TTCGAA 2 cut(s) 479, 754
AvaI CYCGRG 1 cut(s) 3034
AvaII GGWCC 6 cut(s) 1571, 2391, 2464, 2581, 2862, 3161
BaeGI GKGCMC 1 cut(s) 1810
BaeI ACNNNNGTAYC 2 cut(s) 2777, 2810
BamHI GGATCC 1 cut(s) 1006
BanI GGYRCC 2 cut(s) 2035, 2665
BbsI GAAGAC 2 cut(s) 2891, 3221
Bbv12I GWGCWC 3 cut(s) 1810, 2524, 3046
BceAI ACGGC 2 cut(s) 1313, 2653
BciT130I CCWGG 3 cut(s) 66, 1827, 2313
BciVI GTATCC 2 cut(s) 449, 1424
BcnI CCSGG 1 cut(s) 3354
BcoDI GTCTC 4 cut(s) 804, 1511, 2147, 2796
BfaI CTAG 8 cut(s) 372, 387, 1052, 1127, 1772, 2447, 2850, 2873
BfmI CTRYAG 4 cut(s) 1337, 1890, 2166, 3004
BfoI RGCGCY 1 cut(s) 397
BfuAI ACCTGC 2 cut(s) 482, 1106
BfuI GTATCC 2 cut(s) 449, 1424
BmcAI AGTACT 1 cut(s) 1608
Bme1390I CCNGG 4 cut(s) 66, 1827, 2313, 3354
Bme18I GGWCC 6 cut(s) 1571, 2391, 2464, 2581, 2862, 3161
BmeT110I CYCGRG 1 cut(s) 3034
BmgBI CACGTC 1 cut(s) 1014
BmgT120I GGNCC 9 cut(s) 1571, 2391, 2464, 2536, 2581, 2685, 2862, 2915, 3161
BmrFI CCNGG 4 cut(s) 66, 1827, 2313, 3354
BmrI ACTGGG 1 cut(s) 3021
BmsI GCATC 8 cut(s) 697, 1210, 1765, 2230, 2589, 2701, 3006, 3050
BmuI ACTGGG 1 cut(s) 3021
BpiI GAAGAC 2 cut(s) 2891, 3221
BplI GAGNNNNNCTC 4 cut(s) 3028, 3060, 3256, 3288
BpmI CTGGAG 2 cut(s) 3051, 3323
Bpu14I TTCGAA 2 cut(s) 479, 754
BpuEI CTTGAG 1 cut(s) 3258
BpuMI CCSGG 1 cut(s) 3354
BsaBI GATNNNNATC 2 cut(s) 213, 1635
BsaJI CCNNGG 7 cut(s) 65, 422, 1138, 1466, 2398, 2475, 3364
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 5 cut(s) 1467, 1680, 2400, 2509, 2694
Bse118I RCCGGY 2 cut(s) 404, 1955
Bse1I ACTGG 7 cut(s) 437, 989, 1387, 1573, 2503, 2810, 3016
Bse3DI GCAATG 3 cut(s) 669, 2145, 2818
Bse8I GATNNNNATC 2 cut(s) 213, 1635
BseBI CCWGG 3 cut(s) 66, 1827, 2313
BseDI CCNNGG 7 cut(s) 65, 422, 1138, 1466, 2398, 2475, 3364
BseJI GATNNNNATC 2 cut(s) 213, 1635
BseLI CCNNNNNNNGG 5 cut(s) 1467, 1680, 2400, 2509, 2694
BseMI GCAATG 3 cut(s) 669, 2145, 2818
BseMII CTCAG 9 cut(s) 87, 815, 873, 1512, 1793, 2007, 2142, 2298, 3144
BseNI ACTGG 7 cut(s) 437, 989, 1387, 1573, 2503, 2810, 3016
BseRI GAGGAG 3 cut(s) 1265, 3242, 3260
BseSI GKGCMC 1 cut(s) 1810
BseYI CCCAGC 3 cut(s) 47, 2288, 2865
BsgI GTGCAG 1 cut(s) 2700
Bsh1236I CGCG 1 cut(s) 1653
BshFI GGCC 7 cut(s) 156, 1904, 1959, 2538, 2686, 2917, 3388
BshNI GGYRCC 2 cut(s) 2035, 2665
BsiHKAI GWGCWC 3 cut(s) 1810, 2524, 3046
BsiHKCI CYCGRG 1 cut(s) 3034
BsiSI CCGG 4 cut(s) 231, 405, 1956, 3353
BslFI GGGAC 1 cut(s) 3174
BslI CCNNNNNNNGG 5 cut(s) 1467, 1680, 2400, 2509, 2694
BsmAI GTCTC 4 cut(s) 804, 1511, 2147, 2796
BsmFI GGGAC 1 cut(s) 3174
BsmI GAATGC 3 cut(s) 367, 2347, 2630
BsnI GGCC 7 cut(s) 156, 1904, 1959, 2538, 2686, 2917, 3388
BsoBI CYCGRG 1 cut(s) 3034
Bsp119I TTCGAA 2 cut(s) 479, 754
Bsp1286I GDGCHC 3 cut(s) 1810, 2524, 3046
Bsp1407I TGTACA 1 cut(s) 2331
Bsp143I GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
Bsp19I CCATGG 2 cut(s) 422, 3364
BspACI CCGC 6 cut(s) 261, 280, 362, 1999, 2539, 2952
BspANI GGCC 7 cut(s) 156, 1904, 1959, 2538, 2686, 2917, 3388
BspCNI CTCAG 9 cut(s) 88, 814, 874, 1513, 1792, 2006, 2143, 2299, 3145
BspFNI CGCG 1 cut(s) 1653
BspHI TCATGA 2 cut(s) 1950, 3085
BspMAI CTGCAG 2 cut(s) 1894, 3008
BspMI ACCTGC 2 cut(s) 482, 1106
BspPI GGATC 4 cut(s) 1001, 1014, 2872, 3312
BspQI GCTCTTC 2 cut(s) 2250, 3376
BspT104I TTCGAA 2 cut(s) 479, 754
BspT107I GGYRCC 2 cut(s) 2035, 2665
BsrBI CCGCTC 1 cut(s) 1999
BsrDI GCAATG 3 cut(s) 669, 2145, 2818
BsrFI RCCGGY 2 cut(s) 404, 1955
BsrGI TGTACA 1 cut(s) 2331
BsrI ACTGG 7 cut(s) 437, 989, 1387, 1573, 2503, 2810, 3016
BssAI RCCGGY 2 cut(s) 404, 1955
BssECI CCNNGG 7 cut(s) 65, 422, 1138, 1466, 2398, 2475, 3364
BssMI GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
BssNAI GTATAC 1 cut(s) 2218
BssT1I CCWWGG 6 cut(s) 422, 1138, 1466, 2398, 2475, 3364
Bst1107I GTATAC 1 cut(s) 2218
Bst2UI CCWGG 3 cut(s) 66, 1827, 2313
Bst4CI ACNGT 8 cut(s) 962, 1101, 1438, 1934, 2070, 2167, 2571, 2760
Bst6I CTCTTC 8 cut(s) 29, 751, 921, 1503, 2250, 2433, 2610, 3376
BstAPI GCANNNNNTGC 1 cut(s) 1306
BstAUI TGTACA 1 cut(s) 2331
BstBI TTCGAA 2 cut(s) 479, 754
BstC8I GCNNGC 7 cut(s) 1113, 1456, 2076, 2088, 2295, 2299, 3299
BstDSI CCRYGG 2 cut(s) 422, 3364
BstFNI CGCG 1 cut(s) 1653
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 2 cut(s) 396, 491
BstKTI GATC 9 cut(s) 320, 851, 1009, 1639, 1947, 2412, 2551, 2880, 3320
BstMAI GTCTC 4 cut(s) 804, 1511, 2147, 2796
BstMBI GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
BstNI CCWGG 3 cut(s) 66, 1827, 2313
BstNSI RCATGY 3 cut(s) 125, 2322, 2831
BstSCI CCNGG 4 cut(s) 64, 1825, 2311, 3352
BstSFI CTRYAG 4 cut(s) 1337, 1890, 2166, 3004
BstSLI GKGCMC 1 cut(s) 1810
BstUI CGCG 1 cut(s) 1653
BstV2I GAAGAC 2 cut(s) 2891, 3221
BstX2I RGATCY 3 cut(s) 1006, 2877, 3317
BstXI CCANNNNNNTGG 2 cut(s) 2288, 3133
BstYI RGATCY 3 cut(s) 1006, 2877, 3317
BstZ17I GTATAC 1 cut(s) 2218
BsuI GTATCC 2 cut(s) 449, 1424
BsuRI GGCC 7 cut(s) 156, 1904, 1959, 2538, 2686, 2917, 3388
BtgI CCRYGG 2 cut(s) 422, 3364
BtgZI GCGATG 1 cut(s) 2724
BtrI CACGTC 1 cut(s) 1014
BtsI GCAGTG 6 cut(s) 1307, 1899, 2783, 3046, 3125, 3138
BtsIMutI CAGTG 8 cut(s) 967, 1307, 1899, 2756, 2783, 3046, 3125, 3138
BveI ACCTGC 2 cut(s) 482, 1106
Cac8I GCNNGC 7 cut(s) 1113, 1456, 2076, 2088, 2295, 2299, 3299
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 2 cut(s) 1950, 3085
CfoI GCGC 2 cut(s) 396, 491
Cfr10I RCCGGY 2 cut(s) 404, 1955
Cfr13I GGNCC 9 cut(s) 1571, 2391, 2464, 2536, 2581, 2685, 2862, 2915, 3161
CseI GACGC 1 cut(s) 593
Csp6I GTAC 6 cut(s) 1191, 1591, 1607, 2332, 2500, 2567
CspCI CAANNNNNGTGG 6 cut(s) 19, 54, 1455, 1490, 3129, 3164
CviQI GTAC 6 cut(s) 1191, 1591, 1607, 2332, 2500, 2567
DpnI GATC 9 cut(s) 319, 850, 1008, 1638, 1946, 2411, 2550, 2879, 3319
DpnII GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
DraI TTTAAA 1 cut(s) 939
DrdI GACNNNNNNGTC 1 cut(s) 2650
DseDI GACNNNNNNGTC 1 cut(s) 2650
EaeI YGGCCR 1 cut(s) 1957
Eam1104I CTCTTC 8 cut(s) 29, 751, 921, 1503, 2250, 2433, 2610, 3376
EarI CTCTTC 8 cut(s) 29, 751, 921, 1503, 2250, 2433, 2610, 3376
EciI GGCGGA 1 cut(s) 276
Eco130I CCWWGG 6 cut(s) 422, 1138, 1466, 2398, 2475, 3364
Eco32I GATATC 1 cut(s) 1939
Eco47I GGWCC 6 cut(s) 1571, 2391, 2464, 2581, 2862, 3161
Eco57I CTGAAG 3 cut(s) 2130, 2414, 2903
Eco88I CYCGRG 1 cut(s) 3034
EcoO109I RGGNCCY 2 cut(s) 2862, 3161
EcoRII CCWGG 3 cut(s) 64, 1825, 2311
EcoRV GATATC 1 cut(s) 1939
EcoT14I CCWWGG 6 cut(s) 422, 1138, 1466, 2398, 2475, 3364
EcoT22I ATGCAT 3 cut(s) 1617, 2132, 2347
ErhI CCWWGG 6 cut(s) 422, 1138, 1466, 2398, 2475, 3364
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 1 cut(s) 3174
FauI CCCGC 1 cut(s) 369
FauNDI CATATG 1 cut(s) 1239
FblI GTMKAC 1 cut(s) 2217
FspBI CTAG 8 cut(s) 372, 387, 1052, 1127, 1772, 2447, 2850, 2873
GlaI GCGC 2 cut(s) 395, 490
GsaI CCCAGC 3 cut(s) 51, 2292, 2869
GsuI CTGGAG 2 cut(s) 3051, 3323
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 7 cut(s) 156, 1904, 1959, 2538, 2686, 2917, 3388
HapII CCGG 4 cut(s) 231, 405, 1956, 3353
HgaI GACGC 1 cut(s) 593
HhaI GCGC 2 cut(s) 396, 491
Hin6I GCGC 2 cut(s) 394, 489
HinP1I GCGC 2 cut(s) 394, 489
HincII GTYRAC 2 cut(s) 839, 1097
HindII GTYRAC 2 cut(s) 839, 1097
HinfI GANTC 7 cut(s) 530, 1062, 1631, 2175, 2383, 2741, 3185
HpaII CCGG 4 cut(s) 231, 405, 1956, 3353
HphI GGTGA 8 cut(s) 563, 1142, 1211, 1912, 1946, 2624, 2716, 2869
Hpy166II GTNNAC 8 cut(s) 119, 236, 839, 1097, 1808, 2218, 2334, 3001
Hpy8I GTNNAC 8 cut(s) 119, 236, 839, 1097, 1808, 2218, 2334, 3001
Hpy99I CGWCG 1 cut(s) 1740
HpyAV CCTTC 9 cut(s) 7, 146, 287, 459, 542, 647, 718, 1894, 2974
HpyCH4III ACNGT 8 cut(s) 962, 1101, 1438, 1934, 2070, 2167, 2571, 2760
HpyCH4IV ACGT 5 cut(s) 630, 1013, 1735, 2284, 3194
HpySE526I ACGT 5 cut(s) 630, 1013, 1735, 2284, 3194
HspAI GCGC 2 cut(s) 394, 489
KflI GGGWCCC 1 cut(s) 3161
Kzo9I GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
LguI GCTCTTC 2 cut(s) 2250, 3376
LmnI GCTCC 6 cut(s) 786, 1108, 2874, 2954, 3136, 3205
LweI GCATC 8 cut(s) 697, 1210, 1765, 2230, 2589, 2701, 3006, 3050
MaeI CTAG 8 cut(s) 372, 387, 1052, 1127, 1772, 2447, 2850, 2873
MaeII ACGT 5 cut(s) 630, 1013, 1735, 2284, 3194
MaeIII GTNAC 7 cut(s) 16, 374, 910, 1564, 1683, 1756, 1843
MalI GATC 9 cut(s) 319, 850, 1008, 1638, 1946, 2411, 2550, 2879, 3319
MbiI CCGCTC 1 cut(s) 1999
MboI GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
MflI RGATCY 3 cut(s) 1006, 2877, 3317
MhlI GDGCHC 3 cut(s) 1810, 2524, 3046
MlyI GAGTC 3 cut(s) 539, 2750, 3194
MmeI TCCRAC 3 cut(s) 1354, 2769, 3183
Mph1103I ATGCAT 3 cut(s) 1617, 2132, 2347
MseI TTAA 8 cut(s) 938, 1230, 1587, 1746, 2526, 2543, 2753, 2832
MslI CAYNNNNRTG 5 cut(s) 162, 770, 1425, 1697, 3131
MspI CCGG 4 cut(s) 231, 405, 1956, 3353
MspR9I CCNGG 4 cut(s) 66, 1827, 2313, 3354
Mva1269I GAATGC 3 cut(s) 367, 2347, 2630
MvaI CCWGG 3 cut(s) 66, 1827, 2313
MvnI CGCG 1 cut(s) 1653
NciI CCSGG 1 cut(s) 3354
NcoI CCATGG 2 cut(s) 422, 3364
NdeI CATATG 1 cut(s) 1239
NdeII GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
NmuCI GTSAC 3 cut(s) 1564, 1683, 1756
NsiI ATGCAT 3 cut(s) 1617, 2132, 2347
NspI RCATGY 3 cut(s) 125, 2322, 2831
NspV TTCGAA 2 cut(s) 479, 754
OliI CACNNNNGTG 2 cut(s) 1697, 3131
PagI TCATGA 2 cut(s) 1950, 3085
PciI ACATGT 2 cut(s) 121, 2318
PciSI GCTCTTC 2 cut(s) 2250, 3376
PcsI WCGNNNNNNNCGW 1 cut(s) 2290
PctI GAATGC 3 cut(s) 367, 2347, 2630
PfeI GAWTC 4 cut(s) 1062, 1631, 2175, 2383
PfoI TCCNGGA 1 cut(s) 1825
PleI GAGTC 3 cut(s) 538, 2749, 3193
PpsI GAGTC 3 cut(s) 538, 2749, 3193
PpuMI RGGWCCY 2 cut(s) 2862, 3161
PscI ACATGT 2 cut(s) 121, 2318
PsiI TTATAA 1 cut(s) 87
Psp5II RGGWCCY 2 cut(s) 2862, 3161
Psp6I CCWGG 3 cut(s) 64, 1825, 2311
PspFI CCCAGC 3 cut(s) 47, 2288, 2865
PspGI CCWGG 3 cut(s) 64, 1825, 2311
PspPI GGNCC 9 cut(s) 1571, 2391, 2464, 2536, 2581, 2685, 2862, 2915, 3161
PspPPI RGGWCCY 2 cut(s) 2862, 3161
PstI CTGCAG 2 cut(s) 1894, 3008
PstNI CAGNNNCTG 1 cut(s) 1178
PsuI RGATCY 3 cut(s) 1006, 2877, 3317
RsaI GTAC 6 cut(s) 1192, 1592, 1608, 2333, 2501, 2568
RsaNI GTAC 6 cut(s) 1191, 1591, 1607, 2332, 2500, 2567
RseI CAYNNNNRTG 5 cut(s) 162, 770, 1425, 1697, 3131
SapI GCTCTTC 2 cut(s) 2250, 3376
SaqAI TTAA 8 cut(s) 938, 1230, 1587, 1746, 2526, 2543, 2753, 2832
Sau3AI GATC 9 cut(s) 317, 848, 1006, 1636, 1944, 2409, 2548, 2877, 3317
Sau96I GGNCC 9 cut(s) 1571, 2391, 2464, 2536, 2581, 2685, 2862, 2915, 3161
ScaI AGTACT 1 cut(s) 1608
SchI GAGTC 3 cut(s) 539, 2750, 3194
ScrFI CCNGG 4 cut(s) 66, 1827, 2313, 3354
SduI GDGCHC 3 cut(s) 1810, 2524, 3046
SfaNI GCATC 8 cut(s) 697, 1210, 1765, 2230, 2589, 2701, 3006, 3050
SfcI CTRYAG 4 cut(s) 1337, 1890, 2166, 3004
SfuI TTCGAA 2 cut(s) 479, 754
SinI GGWCC 6 cut(s) 1571, 2391, 2464, 2581, 2862, 3161
SmiMI CAYNNNNRTG 5 cut(s) 162, 770, 1425, 1697, 3131
SmlI CTYRAG 1 cut(s) 3273
SmoI CTYRAG 1 cut(s) 3273
SsiI CCGC 6 cut(s) 261, 280, 362, 1999, 2539, 2952
SspI AATATT 1 cut(s) 2485
SspMI CTAG 8 cut(s) 372, 387, 1052, 1127, 1772, 2447, 2850, 2873
StyD4I CCNGG 4 cut(s) 64, 1825, 2311, 3352
StyI CCWWGG 6 cut(s) 422, 1138, 1466, 2398, 2475, 3364
TaaI ACNGT 8 cut(s) 962, 1101, 1438, 1934, 2070, 2167, 2571, 2760
TaiI ACGT 5 cut(s) 633, 1016, 1738, 2287, 3197
TaqI TCGA 4 cut(s) 479, 754, 1348, 2024
TaqII GACCGA 1 cut(s) 1588
TatI WGTACW 3 cut(s) 1590, 1606, 2331
TauI GCSGC 1 cut(s) 2541
TfiI GAWTC 4 cut(s) 1062, 1631, 2175, 2383
Tru1I TTAA 8 cut(s) 938, 1230, 1587, 1746, 2526, 2543, 2753, 2832
Tru9I TTAA 8 cut(s) 938, 1230, 1587, 1746, 2526, 2543, 2753, 2832
TscAI CASTG 8 cut(s) 967, 1307, 1899, 2763, 2783, 3046, 3125, 3138
TseFI GTSAC 3 cut(s) 1564, 1683, 1756
Tsp45I GTSAC 3 cut(s) 1564, 1683, 1756
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 8 cut(s) 967, 1307, 1899, 2763, 2783, 3046, 3125, 3138
VneI GTGCAC 1 cut(s) 1806
VpaK11BI GGWCC 6 cut(s) 1571, 2391, 2464, 2581, 2862, 3161
XapI RAATTY 9 cut(s) 444, 523, 934, 1078, 1265, 1784, 1821, 2726, 3259
XceI RCATGY 3 cut(s) 125, 2322, 2831
XcmI CCANNNNNNNNNTGG 3 cut(s) 1018, 1796, 2233
XmiI GTMKAC 1 cut(s) 2217
XspI CTAG 8 cut(s) 372, 387, 1052, 1127, 1772, 2447, 2850, 2873
ZrmI AGTACT 1 cut(s) 1608
Zsp2I ATGCAT 3 cut(s) 1617, 2132, 2347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.