RLG00000032644

hemK methyltransferase family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009637
Physical Location & Seq
Reverse (-)
17274866 .. 17276357
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032644

Sequence Viewer

Length: 894 bp
ATGACTAACAAAAGTTCCCGCTTTCAGGGCCATTTAAGCTATCTAGAGCTGGAAACCATTAAAGAATCTTCACTTTCGGTCCGAACTACGAGCCAAGGCAAGCAATGGAGTGATGGTCAGGTCCTATACAAGATGTCCCCCAGAACTGCCCAAATCCGCCTTGTCAGCTCACATCGCGAGGTTTATGAACCGTGCGATGATTCATTTGCTTTAGTAGATGCTCTTTTAGCTGATCAAGCAAACCTGTTACAGCATCAACCAAAATTGTGCCTGGAACTGGGCTGTGGTAGTGGCTATGTTATTACTTCTCTAGCTCTTATTCTCGGCAAGGAGGGTCACTATATTGCAACTGATATCAACCCCCATGCAGTGAGAGTGACTCAGGAGACACTTGAAGCACACGGGGTTCATGCAGAATTGATAAACACTAACATTGCATCAGGACTCGAGAAGCGTCTGGCAGGATTAGTCGATGTAATAGTTGTGAACCCGCCTTATGTACCTACCCCAGAAGATGAAGTGGGTTGTGAAGGGATTGCATCTGCTTGGGCTGGTGGAGAGAATGGTCGTGCTGTGATTGATAAGATACTGCCCGTTGCCGACAATCTTTTGTCAGAGAAAGGCTGGTTGTATATGGTCACGCTTTCAGAAAACAATCCATCAGAGATATGCCTTCAAATGAGAGAGAAGGGCTATGCTTCCAGAATCCTTGTCCAGAGATTGACAGACGAAGAGAGTCTCCAGATCATCAAGTTCTGGCGGGATTTTGATTCTCAATTAGAAGCCAAGGAGATGGCAGAAAACAAAACAGTTGCGTCAAGGGTCATGGAGTCTTTGCTCTCTCAATTTCCTAGAATGCCATTTTTGAGAGGCAATGACAGCAACAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS

Protein Analysis

298

Amino Acids

32.92

Weight (kDa)

5.17

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTS PF05175 84 - 214 4.2e-11 Methyltransferase small domain
Methyltransf_31 PF13847 91 - 235 1.8e-09 Methyltransferase domain
Methyltransf_25 PF13649 91 - 162 2.7e-07 Methyltransferase domain
Methyltransf_12 PF08242 91 - 162 4.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 4 cut(s) 19, 157, 491, 760
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 2 cut(s) 25, 277
AgsI TTSAA 2 cut(s) 395, 677
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 6 cut(s) 39, 49, 168, 230, 314, 891
AluI AGCT 6 cut(s) 39, 49, 168, 230, 314, 891
Alw26I GTCTC 2 cut(s) 380, 743
AlwNI CAGNNNCTG 1 cut(s) 891
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 1 cut(s) 28
AspS9I GGNCC 3 cut(s) 28, 79, 121
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 2 cut(s) 79, 121
BccI CCATC 3 cut(s) 107, 667, 787
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 380, 743
BfaI CTAG 3 cut(s) 44, 311, 852
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 79, 121
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 3 cut(s) 28, 79, 121
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 4 cut(s) 208, 262, 446, 548
BmuI ACTGGG 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 364, 396
BpmI CTGGAG 1 cut(s) 725
BsaJI CCNNGG 2 cut(s) 94, 786
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 277
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 3 cut(s) 110, 432, 880
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 2 cut(s) 94, 786
BseLI CCNNNNNNNGG 2 cut(s) 25, 277
BseMI GCAATG 3 cut(s) 110, 432, 880
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 25, 277
BsmAI GTCTC 2 cut(s) 380, 743
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 446
Bsp143I GATC 2 cut(s) 232, 744
Bsp68I TCGCGA 1 cut(s) 177
BspACI CCGC 4 cut(s) 19, 157, 491, 760
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 1 cut(s) 177
BsrDI GCAATG 3 cut(s) 110, 432, 880
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 2 cut(s) 94, 786
BssMI GATC 2 cut(s) 232, 744
BssT1I CCWWGG 2 cut(s) 94, 786
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 192, 811, 887
Bst6I CTCTTC 1 cut(s) 726
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 1 cut(s) 381
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 2 cut(s) 235, 747
BstMAI GTCTC 2 cut(s) 380, 743
BstMBI GATC 2 cut(s) 232, 744
BstMWI GCNNNNNNNGC 8 cut(s) 27, 36, 165, 174, 227, 236, 879, 888
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 793
BsuRI GGCC 1 cut(s) 30
BtgZI GCGATG 2 cut(s) 158, 210
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
BtuMI TCGCGA 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 101
CaiI CAGNNNCTG 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 28, 79, 121
CpoI CGGWCCG 1 cut(s) 79
CseI GACGC 2 cut(s) 443, 804
Csp6I GTAC 1 cut(s) 500
CspI CGGWCCG 1 cut(s) 79
CviAII CATG 3 cut(s) 365, 410, 826
CviQI GTAC 1 cut(s) 500
DdeI CTNAG 1 cut(s) 381
DpnI GATC 2 cut(s) 234, 746
DpnII GATC 2 cut(s) 232, 744
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 94, 786
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 79, 121
Eco88I CYCGRG 1 cut(s) 446
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 94, 786
ErhI CCWWGG 2 cut(s) 94, 786
FaeI CATG 3 cut(s) 368, 413, 829
FaqI GGGAC 1 cut(s) 121
FatI CATG 3 cut(s) 364, 409, 825
FauI CCCGC 3 cut(s) 26, 498, 753
FbaI TGATCA 1 cut(s) 232
FspBI CTAG 3 cut(s) 44, 311, 852
GsuI CTGGAG 1 cut(s) 725
HaeIII GGCC 1 cut(s) 30
HgaI GACGC 2 cut(s) 443, 804
Hin1II CATG 3 cut(s) 368, 413, 829
HinfI GANTC 8 cut(s) 65, 200, 379, 444, 705, 736, 770, 830
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 4 cut(s) 83, 616, 649, 664
Hpy188III TCNNGA 8 cut(s) 44, 176, 383, 441, 448, 702, 715, 742
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 3 cut(s) 524, 682, 683
HpyCH4III ACNGT 3 cut(s) 192, 811, 887
HpyCH4V TGCA 5 cut(s) 347, 368, 413, 437, 539
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 36, 165, 174, 227, 236, 879, 888
HpyF3I CTNAG 1 cut(s) 381
Hsp92II CATG 3 cut(s) 368, 413, 829
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 2 cut(s) 232, 744
LweI GCATC 4 cut(s) 208, 262, 446, 548
MaeI CTAG 3 cut(s) 44, 311, 852
MaeIII GTNAC 4 cut(s) 246, 335, 376, 637
MalI GATC 2 cut(s) 234, 746
MboI GATC 2 cut(s) 232, 744
MboII GAAGA 3 cut(s) 60, 524, 743
MluCI AATT 4 cut(s) 263, 416, 776, 845
MlyI GAGTC 4 cut(s) 373, 438, 745, 839
MnlI CCTC 3 cut(s) 172, 325, 863
MseI TTAA 2 cut(s) 35, 60
MspR9I CCNGG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 8 cut(s) 27, 36, 165, 174, 227, 236, 879, 888
NdeII GATC 2 cut(s) 232, 744
NlaIII CATG 3 cut(s) 368, 413, 829
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 3 cut(s) 335, 376, 637
NruI TCGCGA 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 446
PctI GAATGC 1 cut(s) 861
PfeI GAWTC 4 cut(s) 65, 200, 705, 770
PleI GAGTC 4 cut(s) 373, 438, 744, 838
PpsI GAGTC 4 cut(s) 373, 438, 744, 838
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspPI GGNCC 3 cut(s) 28, 79, 121
PspPPI RGGWCCY 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 891
RruI TCGCGA 1 cut(s) 177
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
Rsr2I CGGWCCG 1 cut(s) 79
RsrII CGGWCCG 1 cut(s) 79
SaqAI TTAA 2 cut(s) 35, 60
Sau3AI GATC 2 cut(s) 232, 744
Sau96I GGNCC 3 cut(s) 28, 79, 121
SchI GAGTC 4 cut(s) 373, 438, 745, 839
ScrFI CCNGG 1 cut(s) 272
SfaNI GCATC 4 cut(s) 208, 262, 446, 548
Sfr274I CTCGAG 1 cut(s) 446
SinI GGWCC 2 cut(s) 79, 121
SlaI CTCGAG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 4 cut(s) 263, 416, 776, 845
SsiI CCGC 4 cut(s) 19, 157, 491, 760
SspMI CTAG 3 cut(s) 44, 311, 852
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 2 cut(s) 94, 786
TaaI ACNGT 3 cut(s) 192, 811, 887
TaqI TCGA 2 cut(s) 447, 471
TaqII GACCGA 1 cut(s) 67
TasI AATT 4 cut(s) 263, 416, 776, 845
TfiI GAWTC 4 cut(s) 65, 200, 705, 770
Tru1I TTAA 2 cut(s) 35, 60
Tru9I TTAA 2 cut(s) 35, 60
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 3 cut(s) 335, 376, 637
Tsp45I GTSAC 3 cut(s) 335, 376, 637
TspDTI ATGAA 4 cut(s) 192, 201, 398, 531
TspRI CASTG 1 cut(s) 375
VpaK11BI GGWCC 2 cut(s) 79, 121
XbaI TCTAGA 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 446
XspI CTAG 3 cut(s) 44, 311, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.