Rw5G014210

hemK methyltransferase family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009637
Physical Location & Seq
Reverse (-)
16902462 .. 16903223
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014210.1

Sequence Viewer

Length: 762 bp
ATGTCCCCCAGAACTGCCCAAATCCGTCTTGTCAGCTCACATCGCGAGGTTTATGAACCGTGCGATGATTCCTTTGCTTTAGTAGATGCTCTTTTGGCTGATCAAGCAAACCTGTTACAGCATCAACCAAAATTGTGCCTGGAACTGGGCTGTGGTAGTGGCTATGTTATTACTTCTCTAGCTCTTATTCTTGGCAAGGAGGGGCACTATATTGCAACTGATATCAACCCCCATGCGGTGAGAGTGACTCAGGAGACACTAGAAGCACACGGGGTTCATGCAGAATTGATAAACACTGACATTGCATCAGGACTTGAGAAGCGTCTGGCAGGATTAGTTGATGTAATAGTTGTGAACCCGCCTTATGTGCCAACTCCTGAAGATGAAGTGGGTCGTGAAGGGATTGCATCTGCTTGGGCTGGCGGAGAGAATGGTCGTGCTGTGATTGATAAGATACTGCCTGTTGCCGATAATCTTTTGTCAGAGAAAGGCTGGTTGTATATGGTCACGCTTTCAGAAAACAATCCATCAGAGATATGCCTTCAAATGAGAGAGAAGGGCTATGCTTCCAGAATCCTTGTCCAGAGATTGACAGACGAAGAAAGTCTCCAGATCATCAAGTTCTGGCGGGATTTTGATTCTCAATTAGAAGAGAAGGAGATGGCAGAAAACAAAACAGTTCCGTCAAGGGTCATGGAGTCTTTGCTCTCTCAATTTCCTAGAATGCCATTTTTGAGAGGCAATAACAGCAACAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS

Protein Analysis

253

Amino Acids

28.02

Weight (kDa)

4.91

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTS PF05175 40 - 170 1.2e-11 Methyltransferase small domain
Methyltransf_31 PF13847 47 - 191 4.5e-10 Methyltransferase domain
Methyltransf_25 PF13649 47 - 118 5.9e-08 Methyltransferase domain
Methyltransf_12 PF08242 47 - 118 3.2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 45
AciI CCGC 4 cut(s) 236, 359, 423, 628
AcuI CTGAAG 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 145, 235
AgsI TTSAA 1 cut(s) 545
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 3 cut(s) 36, 182, 759
AluI AGCT 3 cut(s) 36, 182, 759
Alw26I GTCTC 2 cut(s) 248, 611
AlwNI CAGNNNCTG 1 cut(s) 759
AsuHPI GGTGA 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 207
BccI CCATC 2 cut(s) 535, 655
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 248, 611
BfaI CTAG 3 cut(s) 179, 260, 720
Bme1390I CCNGG 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 4 cut(s) 76, 130, 314, 416
BmuI ACTGGG 1 cut(s) 155
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpmI CTGGAG 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 335
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 235
Bse1I ACTGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 145, 235
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 145, 235
BsmAI GTCTC 2 cut(s) 248, 611
BsmI GAATGC 1 cut(s) 729
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 100, 612
Bsp68I TCGCGA 1 cut(s) 45
BspACI CCGC 4 cut(s) 236, 359, 423, 628
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 1 cut(s) 150
BssMI GATC 2 cut(s) 100, 612
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 60, 679, 755
Bst6I CTCTTC 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 1 cut(s) 249
BstFNI CGCG 1 cut(s) 45
BstKTI GATC 2 cut(s) 103, 615
BstMAI GTCTC 2 cut(s) 248, 611
BstMBI GATC 2 cut(s) 100, 612
BstMWI GCNNNNNNNGC 6 cut(s) 42, 95, 104, 367, 747, 756
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 45
BtgZI GCGATG 2 cut(s) 26, 78
BtsIMutI CAGTG 1 cut(s) 294
BtuMI TCGCGA 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 421
CaiI CAGNNNCTG 1 cut(s) 759
CseI GACGC 1 cut(s) 311
CviAII CATG 3 cut(s) 233, 278, 694
CviJI RGCY 9 cut(s) 36, 98, 150, 162, 182, 419, 492, 561, 759
CviKI_1 RGCY 9 cut(s) 36, 98, 150, 162, 182, 419, 492, 561, 759
DdeI CTNAG 1 cut(s) 249
DpnI GATC 2 cut(s) 102, 614
DpnII GATC 2 cut(s) 100, 612
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
EciI GGCGGA 1 cut(s) 438
Eco32I GATATC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 223
FaeI CATG 3 cut(s) 236, 281, 697
FatI CATG 3 cut(s) 232, 277, 693
FauI CCCGC 2 cut(s) 366, 621
FbaI TGATCA 1 cut(s) 100
FspBI CTAG 3 cut(s) 179, 260, 720
GsuI CTGGAG 1 cut(s) 593
HgaI GACGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 236, 281, 697
HinfI GANTC 5 cut(s) 68, 247, 573, 638, 698
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 484, 517, 532
Hpy188III TCNNGA 8 cut(s) 44, 251, 309, 377, 395, 570, 583, 610
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 4 cut(s) 392, 550, 551, 649
HpyCH4III ACNGT 3 cut(s) 60, 679, 755
HpyCH4V TGCA 4 cut(s) 215, 281, 305, 407
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 95, 104, 367, 747, 756
HpyF3I CTNAG 1 cut(s) 249
Hsp92II CATG 3 cut(s) 236, 281, 697
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 2 cut(s) 100, 612
LweI GCATC 4 cut(s) 76, 130, 314, 416
MaeI CTAG 3 cut(s) 179, 260, 720
MaeIII GTNAC 3 cut(s) 114, 244, 505
MalI GATC 2 cut(s) 102, 614
MboI GATC 2 cut(s) 100, 612
MboII GAAGA 3 cut(s) 392, 611, 662
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 4 cut(s) 131, 284, 644, 713
MlyI GAGTC 2 cut(s) 241, 707
MnlI CCTC 3 cut(s) 40, 193, 731
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 729
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 6 cut(s) 42, 95, 104, 367, 747, 756
NdeII GATC 2 cut(s) 100, 612
NlaIII CATG 3 cut(s) 236, 281, 697
NmuCI GTSAC 2 cut(s) 244, 505
NruI TCGCGA 1 cut(s) 45
PctI GAATGC 1 cut(s) 729
PfeI GAWTC 3 cut(s) 68, 573, 638
PleI GAGTC 2 cut(s) 241, 706
PpsI GAGTC 2 cut(s) 241, 706
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 759
RruI TCGCGA 1 cut(s) 45
Sau3AI GATC 2 cut(s) 100, 612
SchI GAGTC 2 cut(s) 241, 707
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 207
SetI ASST 5 cut(s) 38, 51, 114, 184, 761
SfaNI GCATC 4 cut(s) 76, 130, 314, 416
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 4 cut(s) 131, 284, 644, 713
SsiI CCGC 4 cut(s) 236, 359, 423, 628
SspMI CTAG 3 cut(s) 179, 260, 720
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 60, 679, 755
TasI AATT 4 cut(s) 131, 284, 644, 713
TfiI GAWTC 3 cut(s) 68, 573, 638
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 2 cut(s) 244, 505
Tsp45I GTSAC 2 cut(s) 244, 505
TspDTI ATGAA 3 cut(s) 69, 266, 399
TspGWI ACGGA 2 cut(s) 14, 672
TspRI CASTG 1 cut(s) 301
XspI CTAG 3 cut(s) 179, 260, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.