pycom05g24750

LSD1 zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007186
Physical Location & Seq
Forward (+)
26272928 .. 26278188
5261 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24750.5

Sequence Viewer

Length: 969 bp
ATGACACAAACCCGACCCAACCGAGATGTCGATGTTACAACAGAACAAAAAAAAATAAGGTTGTCCGTTGAAAATTATCTCTCCGGAATCCCATATCTCCAAAATTCCAACCCATTATTTCCACATCGTCAATCAGCGATATCACAAACGCAAAGAATTTCAACATCGAACCAAACCTCTTCCACATTTCCTTTTCACATCCTCCCAAATTCCCAATCCACAAAGCCCGCGTCTTTCTTTCGACCCGATTCCGCGTTTTGCATCTCATTCCAAACAAAAACCCCAAAAGTTTCATTTTTTTGGTCCCAGATTTTACCTTTCCTCGACCCCCGTCAGCAATCCGATTACGTCGAATTACAGCCCCAGTTGCTCGAGGGGTCGTGGAATTTGAACTTTGTTAACGAAATCAGGTTTCTAAAAATTGCAGCTATGCAGAGCCAGCTTGTGTGTAGTGGGTGTAGGAGCATTCTTCTATATCCTAGAGGAGCTACAAATGTATGCTGTGCATTATGCAATACAATCACCCCAGTCCCTGCTCCTGGGACGGAAATGGCTCAACTTATTTGTGGGGGTTGTAGGACGTTGCTAATGCATACGCGTGGGGCAACAAGTGTGAGGTGCTCCTGTTGTCACACAGTAAATCTTGCTCCAGCATCCAGTCAGGTCGCCCACATCAACTGTGGAAACTGCCGGACAACGCTTATGTATCCATTTGGAGCTCCTTCGGTCAAATGTGCAGTCTGTCACTTTGTTACTAATGCTGGGATGGCCAATATGAGGGTTCCAATTCCAGCGCACAGACCTAATGGGACGGCCAATTCAGGAATGGTGCCCTCTACTTCAGCGGCAATGCCTCATTCTCAGAGCCAGACTGTTGTCGTTGAGAACCCTATGTCTGTTGATGAAAGTGGCAAATTGGTGAGCAATGTCGTCGTTGGTGTCACACGGGGAAAAACTAGCACAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-LSD1

Protein Analysis

323

Amino Acids

35.06

Weight (kDa)

9.19

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 829
AccII CGCG 3 cut(s) 230, 254, 598
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 3 cut(s) 228, 252, 845
AcoI YGGCCR 2 cut(s) 768, 813
AcsI RAATTY 4 cut(s) 103, 156, 208, 385
AcuI CTGAAG 1 cut(s) 825
AdeI CACNNNGTG 1 cut(s) 966
AfiI CCNNNNNNNGG 2 cut(s) 539, 777
AflIII ACRYGT 1 cut(s) 596
AgsI TTSAA 3 cut(s) 71, 162, 391
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 4 cut(s) 428, 442, 488, 719
AluI AGCT 4 cut(s) 428, 442, 488, 719
Alw21I GWGCWC 2 cut(s) 623, 721
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 2 cut(s) 768, 813
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 4 cut(s) 103, 156, 208, 385
ArsI GACNNNNNNTTYG 4 cut(s) 215, 247, 723, 755
AspLEI GCGC 1 cut(s) 796
AspS9I GGNCC 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 514, 931
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 834
BalI TGGCCA 1 cut(s) 770
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 721
Bbv12I GWGCWC 2 cut(s) 623, 721
BbvI GCAGC 1 cut(s) 437
BccI CCATC 1 cut(s) 760
BceAI ACGGC 1 cut(s) 828
BcgI CGANNNNNNTGC 2 cut(s) 913, 947
BciT130I CCWGG 1 cut(s) 540
BciVI GTATCC 1 cut(s) 717
BfaI CTAG 2 cut(s) 480, 957
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 2 cut(s) 426, 846
BlsI GCNGC 2 cut(s) 427, 847
Bme1390I CCNGG 1 cut(s) 540
Bme18I GGWCC 1 cut(s) 303
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 3 cut(s) 305, 783, 831
BmrFI CCNGG 1 cut(s) 540
BmrI ACTGGG 2 cut(s) 358, 521
BmsI GCATC 2 cut(s) 270, 662
BmuI ACTGGG 2 cut(s) 358, 521
BoxI GACNNNNGTC 2 cut(s) 330, 875
BpmI CTGGAG 1 cut(s) 633
BsaJI CCNNGG 1 cut(s) 539
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 777
Bse1I ACTGG 3 cut(s) 364, 527, 657
Bse3DI GCAATG 2 cut(s) 855, 931
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 3 cut(s) 198, 653, 771
BseLI CCNNNNNNNGG 2 cut(s) 539, 777
BseMI GCAATG 2 cut(s) 855, 931
BseMII CTCAG 1 cut(s) 875
BseNI ACTGG 3 cut(s) 364, 527, 657
BseRI GAGGAG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 834
BseXI GCAGC 1 cut(s) 437
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 756
Bsh1236I CGCG 3 cut(s) 230, 254, 598
BshFI GGCC 2 cut(s) 770, 815
BshNI GGYRCC 1 cut(s) 829
BsiHKAI GWGCWC 2 cut(s) 623, 721
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 2 cut(s) 84, 691
BslFI GGGAC 4 cut(s) 289, 515, 556, 823
BslI CCNNNNNNNGG 2 cut(s) 539, 777
BsmFI GGGAC 4 cut(s) 289, 515, 556, 823
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 2 cut(s) 770, 815
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 623, 721, 834
Bsp13I TCCGGA 1 cut(s) 83
BspACI CCGC 3 cut(s) 228, 252, 845
BspANI GGCC 2 cut(s) 770, 815
BspCNI CTCAG 1 cut(s) 874
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 3 cut(s) 230, 254, 598
BspLI GGNNCC 3 cut(s) 305, 783, 831
BspT107I GGYRCC 1 cut(s) 829
BsrDI GCAATG 2 cut(s) 855, 931
BsrI ACTGG 3 cut(s) 364, 527, 657
BssECI CCNNGG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 3 cut(s) 637, 680, 874
Bst6I CTCTTC 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 228, 440
BstDEI CTNAG 1 cut(s) 861
BstF5I GGATG 3 cut(s) 198, 653, 771
BstFNI CGCG 3 cut(s) 230, 254, 598
BstHHI GCGC 1 cut(s) 796
BstMWI GCNNNNNNNGC 3 cut(s) 367, 439, 767
BstNI CCWGG 1 cut(s) 540
BstPAI GACNNNNGTC 2 cut(s) 330, 875
BstSCI CCNGG 1 cut(s) 538
BstSLI GKGCMC 1 cut(s) 834
BstUI CGCG 3 cut(s) 230, 254, 598
BstV1I GCAGC 1 cut(s) 437
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 2 cut(s) 770, 815
BtsCI GGATG 3 cut(s) 198, 653, 771
Cac8I GCNNGC 2 cut(s) 228, 440
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 1 cut(s) 796
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 219
DdeI CTNAG 1 cut(s) 861
DraIII CACNNNGTG 1 cut(s) 966
EaeI YGGCCR 2 cut(s) 768, 813
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 721
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 538
EcoRV GATATC 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 721
FaiI YATR 9 cut(s) 94, 431, 475, 499, 511, 594, 704, 776, 893
FaqI GGGAC 4 cut(s) 289, 515, 556, 823
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 426, 846
FokI GGATG 3 cut(s) 185, 640, 778
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 2 cut(s) 426, 846
FspBI CTAG 2 cut(s) 480, 957
GlaI GCGC 1 cut(s) 795
GluI GCNGC 2 cut(s) 426, 846
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 2 cut(s) 770, 815
HapII CCGG 2 cut(s) 84, 691
HgaI GACGC 1 cut(s) 219
HhaI GCGC 1 cut(s) 796
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 2 cut(s) 87, 248
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 2 cut(s) 84, 691
HphI GGTGA 2 cut(s) 514, 931
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 2 cut(s) 343, 864
Hpy188III TCNNGA 2 cut(s) 84, 822
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 2 cut(s) 353, 935
HpyAV CCTTC 1 cut(s) 732
HpyCH4III ACNGT 3 cut(s) 637, 680, 874
HpyCH4IV ACGT 3 cut(s) 348, 581, 965
HpyCH4V TGCA 7 cut(s) 261, 425, 433, 506, 513, 592, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 367, 439, 767
HpyF3I CTNAG 1 cut(s) 861
HpySE526I ACGT 3 cut(s) 348, 581, 965
HspAI GCGC 1 cut(s) 794
Kpn2I TCCGGA 1 cut(s) 83
KspAI GTTAAC 1 cut(s) 400
LmnI GCTCC 7 cut(s) 462, 485, 541, 626, 652, 716, 724
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 2 cut(s) 270, 662
MaeI CTAG 2 cut(s) 480, 957
MaeII ACGT 3 cut(s) 348, 581, 965
MaeIII GTNAC 5 cut(s) 34, 629, 743, 751, 940
MboII GAAGA 2 cut(s) 171, 461
MhlI GDGCHC 3 cut(s) 623, 721, 834
MlsI TGGCCA 1 cut(s) 770
MluI ACGCGT 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 770
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 9 cut(s) 187, 212, 332, 367, 476, 609, 771, 844, 864
Mox20I TGGCCA 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 594
MroI TCCGGA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 770
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 597
Msp20I TGGCCA 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 84, 691
MspR9I CCNGG 1 cut(s) 540
Mva1269I GAATGC 1 cut(s) 465
MvaI CCWGG 1 cut(s) 540
MvnI CGCG 3 cut(s) 230, 254, 598
MwoI GCNNNNNNNGC 3 cut(s) 367, 439, 767
NlaIV GGNNCC 3 cut(s) 305, 783, 831
NmuCI GTSAC 3 cut(s) 629, 743, 940
NsiI ATGCAT 1 cut(s) 594
PaeR7I CTCGAG 1 cut(s) 371
PctI GAATGC 1 cut(s) 465
PfeI GAWTC 2 cut(s) 87, 248
PkrI GCNGC 2 cut(s) 427, 847
PshAI GACNNNNGTC 2 cut(s) 330, 875
Psp124BI GAGCTC 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 538
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 3 cut(s) 305, 783, 831
PspPI GGNCC 1 cut(s) 303
PspXI VCTCGAGB 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 533
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 721
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 2 cut(s) 426, 846
Sau96I GGNCC 1 cut(s) 303
ScrFI CCNGG 1 cut(s) 540
SduI GDGCHC 3 cut(s) 623, 721, 834
SfaNI GCATC 2 cut(s) 270, 662
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 1 cut(s) 303
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SsiI CCGC 3 cut(s) 228, 252, 845
SspMI CTAG 2 cut(s) 480, 957
SstI GAGCTC 1 cut(s) 721
StyD4I CCNGG 1 cut(s) 538
TaaI ACNGT 3 cut(s) 637, 680, 874
TaiI ACGT 3 cut(s) 351, 584, 968
TaqI TCGA 6 cut(s) 30, 167, 241, 324, 351, 372
TaqII GACCGA 1 cut(s) 715
TauI GCSGC 1 cut(s) 848
TfiI GAWTC 2 cut(s) 87, 248
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseFI GTSAC 3 cut(s) 629, 743, 940
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 3 cut(s) 629, 743, 940
TspDTI ATGAA 2 cut(s) 282, 918
TspGWI ACGGA 2 cut(s) 55, 560
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 4 cut(s) 103, 156, 208, 385
XcmI CCANNNNNNNNNTGG 2 cut(s) 677, 823
XhoI CTCGAG 1 cut(s) 371
XspI CTAG 2 cut(s) 480, 957
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.