pycom05g25400

Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004719
Physical Location & Seq
Reverse (-)
26763969 .. 26767490
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGAAATACAAATACAATGACGTGGACTTTAAGATAAGACAACCAAATGGGAGATTAGAGACAATGATTTCCTGTTTTTCTTCTGTAAATAAGCACTGCCATTATTTGAACTTTGCCTCTAGAGAGAGAGAGAGAGAGAGAGAGAGCATGGCAATTTTCTGTAGGGTGAGATCACTGAATCATCTTTCCAAATGCATTTCCTCAATCCACCTACTTTCTCTTCTGTCATCTTCAACACGCAGATTTTATTCAGAACAAGTCTCATCCGGTTTTGAGAGTGTTGGGTTTATAGGGCTGGGAAATATGGGATCAAGAATGGCAAATAATCTAATTAAGGCTGGATACCGAGTCGCTGTTCATGACATAAACAGCAATGCTACGAAGAAGTTCTCTGACATGGGAGCTGCAACGAAAGAAACACCTTTAAAAGTTGCTGAAGCTAGTGATGTTGTAATCACAATGCTGCCTTCCTCATCTCACGTATACGATGTTTATACTGGACCTAATGGTTTACTTTGTGGAGGGGAATTACGACCATGGTTGTTAATAGATTCATCCACTATTGATCCACAAACGTCGAGAAAGCTTTCTGTCGCTGTGTCTGACTGTACTTTAAAGGAGAAAAAAGATTATCCGAAGAAGCCTGACATGTTGGATGCCCCTGTATCTGGAGGTGTTCTTGCTGCAGAAGCGGGGACTCTTACTTTCATGGTGGGTGGCTCTGAGGAATCTTATCTGGCAGCAAAGTCCTTATTCCTTGCGATGGGCAAGAACACGATTTATTGTGGTGGATCAGGAAATGGTTCAGCAGCAAAGATCTGCAATAATTTGGCAATGTCTATTAGTATGATTGGTGTTTCTGAATCCCTTGCTCTTGGCCAGGCTCTGGGACTTTCCGCAAGTACTCTGACAAGGGTACTCAACTCTTCTAGTGGTCGCTGTTGGAGTAGTGATAGTTATAATCCGGTTCCTGGAGTGATGGAAGGAGTGCCCTCTTCAAGGAACTATGATGGCGGGTTCGCATGCAAACTTATGGCTAAGGATCTAAACCTTGGTGCAACATCAGCCGAAGAGGTTGGTGTTAGATGCCCGTTGACGTCCCAAGCACAAAAGATATACACTCAGCTCTGCAAGAATGGTCATGAAACCGAGGACTTCTCCTGTGTTTTCCGGCATTATTACTCCGGCAAGAACGAGAGCTAG

Protein Analysis

401

Amino Acids

43.28

Weight (kDa)

8.58

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 92 - 160 2.2e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 94 - 263 5.2e-41 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 94 - 156 1e-06 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 266 - 392 2.7e-26 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AatII GACGTC 1 cut(s) 1100
AccB7I CCANNNNNTGG 1 cut(s) 886
AccI GTMKAC 1 cut(s) 483
AciI CCGC 3 cut(s) 692, 897, 1014
AclWI GGATC 4 cut(s) 316, 560, 799, 1050
AcoI YGGCCR 1 cut(s) 877
AcuI CTGAAG 1 cut(s) 456
AcyI GRCGYC 1 cut(s) 1097
AfaI GTAC 3 cut(s) 610, 904, 918
AfiI CCNNNNNNNGG 5 cut(s) 668, 763, 886, 971, 999
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 3 cut(s) 109, 234, 999
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 879, 970
AluBI AGCT 5 cut(s) 404, 440, 586, 1126, 1200
AluI AGCT 5 cut(s) 404, 440, 586, 1126, 1200
Alw26I GTCTC 2 cut(s) 53, 265
AlwI GGATC 4 cut(s) 316, 560, 799, 1050
AlwNI CAGNNNCTG 1 cut(s) 886
AoxI GGCC 1 cut(s) 877
ApeKI GCWGC 5 cut(s) 404, 463, 683, 740, 809
Asp700I GAANNNNTTC 3 cut(s) 386, 586, 802
AspS9I GGNCC 1 cut(s) 500
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 993
BalI TGGCCA 1 cut(s) 879
BbvI GCAGC 5 cut(s) 391, 450, 670, 752, 821
BccI CCATC 3 cut(s) 757, 973, 1004
BciT130I CCWGG 2 cut(s) 881, 972
BciVI GTATCC 1 cut(s) 335
BcoDI GTCTC 2 cut(s) 53, 265
BfaI CTAG 4 cut(s) 120, 441, 930, 1201
BfmI CTRYAG 2 cut(s) 160, 684
BfuI GTATCC 1 cut(s) 335
BglII AGATCT 1 cut(s) 816
BisI GCNGC 5 cut(s) 405, 464, 684, 741, 810
BlsI GCNGC 5 cut(s) 406, 465, 685, 742, 811
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 2 cut(s) 881, 972
Bme18I GGWCC 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 1 cut(s) 969
BmrFI CCNGG 2 cut(s) 881, 972
BmsI GCATC 2 cut(s) 646, 1076
BplI GAGNNNNNCTC 2 cut(s) 1142, 1174
BpmI CTGGAG 2 cut(s) 690, 993
Bpu10I CCTNAGC 1 cut(s) 1038
BsaAI YACGTR 1 cut(s) 481
BsaHI GRCGYC 1 cut(s) 1097
BsaJI CCNNGG 3 cut(s) 536, 1051, 1149
BsaWI WCCGGW 2 cut(s) 266, 964
Bsc4I CCNNNNNNNGG 5 cut(s) 668, 763, 886, 971, 999
Bse1I ACTGG 1 cut(s) 502
Bse3DI GCAATG 2 cut(s) 379, 840
BseBI CCWGG 2 cut(s) 881, 972
BseDI CCNNGG 3 cut(s) 536, 1051, 1149
BseGI GGATG 3 cut(s) 263, 554, 661
BseLI CCNNNNNNNGG 5 cut(s) 668, 763, 886, 971, 999
BseMI GCAATG 2 cut(s) 379, 840
BseMII CTCAG 2 cut(s) 714, 1136
BseNI ACTGG 1 cut(s) 502
BseSI GKGCMC 1 cut(s) 993
BseXI GCAGC 5 cut(s) 391, 450, 670, 752, 821
BseYI CCCAGC 1 cut(s) 295
BshFI GGCC 1 cut(s) 879
BsiSI CCGG 4 cut(s) 267, 965, 1171, 1185
BslFI GGGAC 3 cut(s) 709, 903, 1084
BslI CCNNNNNNNGG 5 cut(s) 668, 763, 886, 971, 999
BsmAI GTCTC 2 cut(s) 53, 265
BsmFI GGGAC 3 cut(s) 709, 903, 1084
BsnI GGCC 1 cut(s) 879
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
Bsp19I CCATGG 1 cut(s) 536
BspACI CCGC 3 cut(s) 692, 897, 1014
BspANI GGCC 1 cut(s) 879
BspCNI CTCAG 2 cut(s) 715, 1135
BspHI TCATGA 2 cut(s) 358, 1141
BspLI GGNNCC 1 cut(s) 969
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 4 cut(s) 316, 560, 799, 1050
BsrDI GCAATG 2 cut(s) 379, 840
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 3 cut(s) 536, 1051, 1149
BssMI GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
BssNAI GTATAC 1 cut(s) 484
BssNI GRCGYC 1 cut(s) 1097
BssT1I CCWWGG 2 cut(s) 536, 1051
Bst1107I GTATAC 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 881, 972
Bst4CI ACNGT 1 cut(s) 608
Bst6I CTCTTC 4 cut(s) 225, 931, 1000, 1065
BstACI GRCGYC 1 cut(s) 1097
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 1024
BstDEI CTNAG 3 cut(s) 723, 1038, 1122
BstDSI CCRYGG 1 cut(s) 536
BstENI CCTNNNNNAGG 1 cut(s) 997
BstF5I GGATG 3 cut(s) 263, 554, 661
BstKTI GATC 6 cut(s) 173, 311, 568, 794, 819, 1045
BstMAI GTCTC 2 cut(s) 53, 265
BstMBI GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 689, 1064
BstNI CCWGG 2 cut(s) 881, 972
BstNSI RCATGY 2 cut(s) 652, 1026
BstSCI CCNGG 2 cut(s) 879, 970
BstSFI CTRYAG 2 cut(s) 160, 684
BstSLI GKGCMC 1 cut(s) 993
BstV1I GCAGC 5 cut(s) 391, 450, 670, 752, 821
BstX2I RGATCY 2 cut(s) 816, 1042
BstYI RGATCY 2 cut(s) 816, 1042
BstZ17I GTATAC 1 cut(s) 484
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 536
BtgZI GCGATG 1 cut(s) 776
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 263, 554, 661
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 94, 173
Cac8I GCNNGC 1 cut(s) 1024
CaiI CAGNNNCTG 1 cut(s) 886
CciI TCATGA 2 cut(s) 358, 1141
Cfr13I GGNCC 1 cut(s) 500
Csp6I GTAC 3 cut(s) 609, 903, 917
CviAII CATG 8 cut(s) 148, 359, 397, 537, 649, 709, 1023, 1142
CviQI GTAC 3 cut(s) 609, 903, 917
DdeI CTNAG 3 cut(s) 723, 1038, 1122
DpnI GATC 6 cut(s) 172, 310, 567, 793, 818, 1044
DpnII GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
DraI TTTAAA 2 cut(s) 426, 615
EaeI YGGCCR 1 cut(s) 877
Eam1104I CTCTTC 4 cut(s) 225, 931, 1000, 1065
EarI CTCTTC 4 cut(s) 225, 931, 1000, 1065
Eco130I CCWWGG 2 cut(s) 536, 1051
Eco47I GGWCC 1 cut(s) 500
Eco57I CTGAAG 1 cut(s) 456
EcoNI CCTNNNNNAGG 1 cut(s) 997
EcoRII CCWGG 2 cut(s) 879, 970
EcoT14I CCWWGG 2 cut(s) 536, 1051
EcoT22I ATGCAT 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 536, 1051
FaeI CATG 8 cut(s) 151, 362, 400, 540, 652, 712, 1026, 1145
FaqI GGGAC 3 cut(s) 709, 903, 1084
FatI CATG 8 cut(s) 147, 358, 396, 536, 648, 708, 1022, 1141
FauI CCCGC 2 cut(s) 685, 1007
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 5 cut(s) 405, 464, 684, 741, 810
FokI GGATG 3 cut(s) 250, 541, 668
Fsp4HI GCNGC 5 cut(s) 405, 464, 684, 741, 810
FspBI CTAG 4 cut(s) 120, 441, 930, 1201
GluI GCNGC 5 cut(s) 405, 464, 684, 741, 810
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 690, 993
HaeIII GGCC 1 cut(s) 879
HapII CCGG 4 cut(s) 267, 965, 1171, 1185
Hin1I GRCGYC 1 cut(s) 1097
Hin1II CATG 8 cut(s) 151, 362, 400, 540, 652, 712, 1026, 1145
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 6 cut(s) 178, 348, 551, 697, 728, 863
HpaII CCGG 4 cut(s) 267, 965, 1171, 1185
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 25, 484, 512, 1095
Hpy188I TCNGA 7 cut(s) 253, 394, 604, 636, 724, 862, 909
Hpy188III TCNNGA 7 cut(s) 120, 312, 359, 579, 669, 795, 1142
Hpy8I GTNNAC 4 cut(s) 25, 484, 512, 1095
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 2 cut(s) 477, 977
HpyCH4III ACNGT 1 cut(s) 608
HpyCH4IV ACGT 4 cut(s) 21, 480, 575, 1097
HpyCH4V TGCA 7 cut(s) 195, 407, 686, 822, 1026, 1058, 1131
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 1064
HpyF3I CTNAG 3 cut(s) 723, 1038, 1122
HpySE526I ACGT 4 cut(s) 21, 480, 575, 1097
Hsp92I GRCGYC 1 cut(s) 1097
Hsp92II CATG 8 cut(s) 151, 362, 400, 540, 652, 712, 1026, 1145
Kzo9I GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 5 cut(s) 391, 450, 670, 752, 821
LweI GCATC 2 cut(s) 646, 1076
MaeI CTAG 4 cut(s) 120, 441, 930, 1201
MaeII ACGT 4 cut(s) 21, 480, 575, 1097
MalI GATC 6 cut(s) 172, 310, 567, 793, 818, 1044
MboI GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
MboII GAAGA 8 cut(s) 72, 212, 222, 394, 649, 918, 987, 1082
MflI RGATCY 2 cut(s) 816, 1042
MhlI GDGCHC 1 cut(s) 993
MlsI TGGCCA 1 cut(s) 879
MluCI AATT 4 cut(s) 153, 330, 527, 826
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 2 cut(s) 357, 691
MmeI TCCRAC 2 cut(s) 633, 923
MnlI CCTC 9 cut(s) 127, 211, 481, 515, 665, 718, 1003, 1066, 1144
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 197
MroXI GAANNNNTTC 3 cut(s) 386, 586, 802
MscI TGGCCA 1 cut(s) 879
MseI TTAA 5 cut(s) 30, 333, 425, 545, 614
Msp20I TGGCCA 1 cut(s) 879
MspI CCGG 4 cut(s) 267, 965, 1171, 1185
MspR9I CCNGG 2 cut(s) 881, 972
MvaI CCWGG 2 cut(s) 881, 972
MwoI GCNNNNNNNGC 2 cut(s) 689, 1064
NcoI CCATGG 1 cut(s) 536
NdeII GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
NlaIII CATG 8 cut(s) 151, 362, 400, 540, 652, 712, 1026, 1145
NlaIV GGNNCC 1 cut(s) 969
NsiI ATGCAT 1 cut(s) 197
NspI RCATGY 2 cut(s) 652, 1026
PaeI GCATGC 1 cut(s) 1026
PagI TCATGA 2 cut(s) 358, 1141
PciI ACATGT 1 cut(s) 648
PdmI GAANNNNTTC 3 cut(s) 386, 586, 802
PfeI GAWTC 4 cut(s) 178, 551, 728, 863
PflMI CCANNNNNTGG 1 cut(s) 886
PfoI TCCNGGA 1 cut(s) 970
PkrI GCNGC 5 cut(s) 406, 465, 685, 742, 811
PleI GAGTC 2 cut(s) 356, 691
PpsI GAGTC 2 cut(s) 356, 691
Ppu21I YACGTR 1 cut(s) 481
PscI ACATGT 1 cut(s) 648
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 879, 970
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 2 cut(s) 879, 970
PspN4I GGNNCC 1 cut(s) 969
PspPI GGNCC 1 cut(s) 500
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 886
PsuI RGATCY 2 cut(s) 816, 1042
RsaI GTAC 3 cut(s) 610, 904, 918
RsaNI GTAC 3 cut(s) 609, 903, 917
SaqAI TTAA 5 cut(s) 30, 333, 425, 545, 614
SatI GCNGC 5 cut(s) 405, 464, 684, 741, 810
Sau3AI GATC 6 cut(s) 170, 308, 565, 791, 816, 1042
Sau96I GGNCC 1 cut(s) 500
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 2 cut(s) 357, 691
ScrFI CCNGG 2 cut(s) 881, 972
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 2 cut(s) 646, 1076
SfcI CTRYAG 2 cut(s) 160, 684
SinI GGWCC 1 cut(s) 500
SphI GCATGC 1 cut(s) 1026
Sse9I AATT 4 cut(s) 153, 330, 527, 826
SsiI CCGC 3 cut(s) 692, 897, 1014
SspMI CTAG 4 cut(s) 120, 441, 930, 1201
StyD4I CCNGG 2 cut(s) 879, 970
StyI CCWWGG 2 cut(s) 536, 1051
TaaI ACNGT 1 cut(s) 608
TaiI ACGT 4 cut(s) 24, 483, 578, 1100
TaqI TCGA 1 cut(s) 578
TasI AATT 4 cut(s) 153, 330, 527, 826
TatI WGTACW 2 cut(s) 608, 902
TfiI GAWTC 4 cut(s) 178, 551, 728, 863
Tru1I TTAA 5 cut(s) 30, 333, 425, 545, 614
Tru9I TTAA 5 cut(s) 30, 333, 425, 545, 614
TscAI CASTG 2 cut(s) 101, 180
TseI GCWGC 5 cut(s) 404, 463, 683, 740, 809
TspDTI ATGAA 5 cut(s) 17, 347, 543, 697, 1158
TspRI CASTG 2 cut(s) 101, 180
Van91I CCANNNNNTGG 1 cut(s) 886
VpaK11BI GGWCC 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 997
XbaI TCTAGA 1 cut(s) 119
XceI RCATGY 2 cut(s) 652, 1026
XmiI GTMKAC 1 cut(s) 483
XmnI GAANNNNTTC 3 cut(s) 386, 586, 802
XspI CTAG 4 cut(s) 120, 441, 930, 1201
ZraI GACGTC 1 cut(s) 1098
ZrmI AGTACT 1 cut(s) 904
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.