pycom05g26710
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012414
Physical Location & Seq
Reverse (-)
27872625 .. 27874298
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
ATGTCTGATCATGAACCTAAAGACCTCTACTACCATGACCTATTTCAGTATGAAGCTCATGAACACCTCAATGGTGGGATGGTCAGCATCAACGACCACCAAAATTTGCAAGGGTTTGATGTGAATCCTTCATACATGAACTTCACAGAGTGTTCCAAAGGATCTGTGGACTATAACTCACTTGCCACGGCTTTTGGCTTGTCATCTTCAACATCTGAAGCACTATTTTCATCCGTTGAAGGCAATCAAAACGCTGTTGATGATCTTGGAGGCGGCGGCGGTGAAACTCCTTTGACGCCAAACTCTTCAGTGTCTTCTTCTTCAGCTGAGGCTGATGCTGATAAGGACTGTGGTAAGAATAAGAAAGATAGGCAGCCAAAAGGGTCATCAGAAGATGGAGATAGCTCTAAGAAAGTGAGCAAACCAGCAAAAAAGAAGGGAGAGAAAAAGCAAAAAGAGCCACGATTTGCCTTCATGACCAAGACTGAGGTTGATCATCTAGAAGATGGATACAGATGGAGAAAATATGGACAAAAAGCTGTGAAAAATAGTCCATTTCCAAGAAGCTACTACCGATGCACGACACAAAAATGCGGGGTCAAGAAACGCGTAGAGAGGTCGTACGAGGACCCATCTACTGTGATTACGACATATGAAGGCCAGCACAACCACCAACTTCCAGCAAACCTAAGAGGAAATGCGGCTGCAGCATTGTTCCCACCTTCTATGCTAAACCTGTCGAACTTGGCTGCTGGTGGCCCGAGCTCTACCTTTCCTCAGGAACTCTTGTTTCAAATGCCCCAATACATGATCAGTAGCAATGTTAATCAGGGATCGTCACGAAACCCTAATCATCATCTGCAGCAATATCAACTGCCTCATGCAGCTGCTGATCAGTATGGACATTTGCAGGACTTTGCTCCTTCCATGTTCCTCAAACAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

317

Amino Acids

34.95

Weight (kDa)

6.46

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 168 - 225 8.8e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 609
AciI CCGC 5 cut(s) 273, 276, 279, 594, 701
AclWI GGATC 2 cut(s) 169, 841
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 3 cut(s) 237, 291, 306
AcyI GRCGYC 1 cut(s) 296
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 3 cut(s) 210, 239, 794
AluBI AGCT 7 cut(s) 56, 326, 405, 539, 567, 765, 887
AluI AGCT 7 cut(s) 56, 326, 405, 539, 567, 765, 887
Alw21I GWGCWC 1 cut(s) 767
AlwI GGATC 2 cut(s) 169, 841
AlwNI CAGNNNCTG 1 cut(s) 890
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 2 cut(s) 658, 757
ApeKI GCWGC 7 cut(s) 373, 704, 707, 749, 862, 884, 887
ApoI RAATTY 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 628, 758
AsuHPI GGTGA 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 1 cut(s) 628
AxyI CCTNAGG 1 cut(s) 777
BanII GRGCYC 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 767
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 7 cut(s) 385, 691, 719, 736, 874, 874, 896
BccI CCATC 5 cut(s) 73, 389, 500, 510, 640
BceAI ACGGC 1 cut(s) 204
BciVI GTATCC 1 cut(s) 503
BclI TGATCA 4 cut(s) 7, 493, 810, 892
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 2 cut(s) 705, 860
BfuI GTATCC 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 628
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 2 cut(s) 628, 758
BmiI GGNNCC 1 cut(s) 630
BmsI GCATC 3 cut(s) 96, 325, 566
BpiI GAAGAC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 186
Bse21I CCTNAGG 1 cut(s) 777
Bse3DI GCAATG 1 cut(s) 826
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 84, 230
BseJI GATNNNNATC 1 cut(s) 123
BseMI GCAATG 1 cut(s) 826
BseMII CTCAG 3 cut(s) 318, 477, 791
BseXI GCAGC 7 cut(s) 385, 691, 719, 736, 874, 874, 896
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 2 cut(s) 660, 759
BsiHKAI GWGCWC 1 cut(s) 767
BsiHKCI CYCGRG 1 cut(s) 760
BsiWI CGTACG 1 cut(s) 621
BsnI GGCC 2 cut(s) 660, 759
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 767
Bsp143I GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
BspACI CCGC 5 cut(s) 273, 276, 279, 594, 701
BspANI GGCC 2 cut(s) 660, 759
BspCNI CTCAG 3 cut(s) 319, 478, 790
BspFNI CGCG 1 cut(s) 609
BspHI TCATGA 3 cut(s) 10, 58, 474
BspLI GGNNCC 1 cut(s) 630
BspMAI CTGCAG 2 cut(s) 709, 864
BspPI GGATC 2 cut(s) 169, 841
BsrDI GCAATG 1 cut(s) 826
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
BssNI GRCGYC 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 350, 640
Bst6I CTCTTC 1 cut(s) 310
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 5 cut(s) 327, 408, 486, 689, 777
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 84, 230
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 7 cut(s) 10, 164, 265, 496, 813, 836, 895
BstMBI GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
BstMWI GCNNNNNNNGC 2 cut(s) 457, 707
BstSFI CTRYAG 2 cut(s) 705, 860
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 7 cut(s) 385, 691, 719, 736, 874, 874, 896
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
Bsu36I CCTNAGG 1 cut(s) 777
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 2 cut(s) 660, 759
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 84, 230
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 662
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 3 cut(s) 10, 58, 474
Cfr13I GGNCC 2 cut(s) 628, 758
CseI GACGC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 622
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 8 cut(s) 11, 35, 59, 136, 475, 808, 881, 928
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 5 cut(s) 327, 408, 486, 689, 777
DpnI GATC 7 cut(s) 9, 163, 264, 495, 812, 835, 894
DpnII GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
DraIII CACNNNGTG 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 765
Eco24I GRGCYC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 765
Eco57I CTGAAG 3 cut(s) 237, 291, 306
Eco81I CCTNAGG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 767
FaeI CATG 8 cut(s) 14, 38, 62, 139, 478, 811, 884, 931
FatI CATG 8 cut(s) 10, 34, 58, 135, 474, 807, 880, 927
FauI CCCGC 1 cut(s) 587
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 4 cut(s) 7, 493, 810, 892
FokI GGATG 2 cut(s) 91, 217
FriOI GRGCYC 1 cut(s) 767
FspBI CTAG 1 cut(s) 500
HaeIII GGCC 2 cut(s) 660, 759
HgaI GACGC 1 cut(s) 304
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 8 cut(s) 14, 38, 62, 139, 478, 811, 884, 931
HinfI GANTC 1 cut(s) 124
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 3 cut(s) 7, 217, 391
Hpy188III TCNNGA 7 cut(s) 11, 59, 475, 500, 601, 779, 840
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 7 cut(s) 138, 233, 430, 481, 650, 732, 933
HpyCH4III ACNGT 2 cut(s) 350, 640
HpyCH4V TGCA 6 cut(s) 109, 579, 707, 862, 884, 910
HpyF10VI GCNNNNNNNGC 2 cut(s) 457, 707
HpyF3I CTNAG 5 cut(s) 327, 408, 486, 689, 777
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 8 cut(s) 14, 38, 62, 139, 478, 811, 884, 931
Ksp22I TGATCA 4 cut(s) 7, 493, 810, 892
Kzo9I GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
LmnI GCTCC 1 cut(s) 925
LpnPI CCDG 8 cut(s) 438, 674, 693, 738, 749, 764, 815, 896
Lsp1109I GCAGC 7 cut(s) 385, 691, 719, 736, 874, 874, 896
LweI GCATC 3 cut(s) 96, 325, 566
MaeI CTAG 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 837
MalI GATC 7 cut(s) 9, 163, 264, 495, 812, 835, 894
MboI GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
MboII GAAGA 7 cut(s) 198, 297, 306, 309, 312, 404, 515
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 767
MluCI AATT 1 cut(s) 103
MluI ACGCGT 1 cut(s) 607
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 2 cut(s) 69, 589
MspA1I CMGCKG 2 cut(s) 326, 887
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 457, 707
NdeI CATATG 1 cut(s) 652
NdeII GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
NlaIII CATG 8 cut(s) 14, 38, 62, 139, 478, 811, 884, 931
NlaIV GGNNCC 1 cut(s) 630
NmuCI GTSAC 1 cut(s) 837
PagI TCATGA 3 cut(s) 10, 58, 474
PfeI GAWTC 1 cut(s) 124
Pfl23II CGTACG 1 cut(s) 621
PpuMI RGGWCCY 1 cut(s) 628
Psp124BI GAGCTC 1 cut(s) 767
Psp5II RGGWCCY 1 cut(s) 628
PspLI CGTACG 1 cut(s) 621
PspN4I GGNNCC 1 cut(s) 630
PspPI GGNCC 2 cut(s) 628, 758
PspPPI RGGWCCY 1 cut(s) 628
PstI CTGCAG 2 cut(s) 709, 864
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 161
PvuII CAGCTG 2 cut(s) 326, 887
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 2 cut(s) 69, 589
SacI GAGCTC 1 cut(s) 767
SaqAI TTAA 1 cut(s) 825
Sau3AI GATC 7 cut(s) 7, 161, 262, 493, 810, 833, 892
Sau96I GGNCC 2 cut(s) 628, 758
SduI GDGCHC 1 cut(s) 767
SfaNI GCATC 3 cut(s) 96, 325, 566
SfcI CTRYAG 2 cut(s) 705, 860
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 2 cut(s) 69, 589
Sse9I AATT 1 cut(s) 103
SsiI CCGC 5 cut(s) 273, 276, 279, 594, 701
SspMI CTAG 1 cut(s) 500
SstI GAGCTC 1 cut(s) 767
TaaI ACNGT 2 cut(s) 350, 640
TaqI TCGA 1 cut(s) 740
TasI AATT 1 cut(s) 103
TauI GCSGC 3 cut(s) 276, 279, 704
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 1 cut(s) 315
TseFI GTSAC 1 cut(s) 837
TseI GCWGC 7 cut(s) 373, 704, 707, 749, 862, 884, 887
Tsp45I GTSAC 1 cut(s) 837
TspDTI ATGAA 8 cut(s) 27, 66, 75, 120, 152, 219, 463, 669
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 628
XapI RAATTY 1 cut(s) 103
XbaI TCTAGA 1 cut(s) 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XspI CTAG 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.