pycom05g28630
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012987
Physical Location & Seq
Reverse (-)
29264734 .. 29267922
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2748 bp
ATGCTTCTCTTCCTCCCCCTCCTCCTCCTCCTGCTGCTGCCGCCACTCCCCGCCACCCTCTCCCTCAACCAAGAAGGCCTCTACCTCCAACACTTCAAGCTCTCCCTCGACGACCCCGACTCCGCCCTCTCCTCCTGGAACGATGCCGACTCCACCCCCTGCAATTGGCTTGGCGTCTCATGCGACGACGCCTCCTCCTCCTCCTCCGTAGTCCCAATTCCCGACTCCTTCGGCCGCTTCCAGAAACTCGAGGTCATCTCTCTCGTCTACAATCTCATCGAAAACACCATCCCGCCCTTTCTCGGCAACATCTCCACCCTGAAAATGCTCAACCTCTCCTACAACCCATTTCACCCGGGTCGGATCCCGGCGGAGCTCGGCAATTTGACGAACCTCGAGGTCCTCTGGCTCACCGAGTGCAACCTAGTCGGCGAGATTCCCGACTCGCTAGGTCAACTCAAAAACCTCAAGGATTTGGACCTCGCCATCAACGGCTTAACCGGCCGGATTCCGCCGTCGCTCAGCGAGCTGATCAGCGTCGTCCAGATTGAGCTCTACAACAACTCATTAACCGGCGAGCTGCCGCCTGGAATGTCGAAATTGACCCGGCTGCGACTTCTCGACGCGTCCATGAACCAGCTGAGCGGGCAGATTCCGGACGAGCTCTGCCGGTTGCCGCTCGAAAGTCTCAACCTTTACGAGAACAACTTAGAGGGGAGCGTGCCGGCGAGCATTGCCAACTCGCCCAACTTGTACGAGGTCAGGCTCTTCCGAAACAAGCTCTCCGGCGAGCTGCCCCAAAATCTCGGGAAGAACTCGCCGCTTAAGTGGTTTGATGTGTCGAGCAACCAGTTCACGGGCACCATTCCGGCGAGTCTGTGCGAGAAGGGACAAATGGAAGAGATTCTAATGCTACACAATGAGTTCTCCGGCGAGATCCCGGCAAGGTTGGGCGAGTGCCAGAGCTTGGCCCGGGTCCGATTGGGTCACAACAGGTTAAGTGGGGAAGTTCCGGTGGGCTTCTGGGGGCTGCCGCGCGTTTACTTGATGGAGCTCGCTGAGAATGAGCTTTCTGGGCCGATAGCGAAGAGCATTGCCGGAGCCACGAACCTCTCGCTATTAATCCTAGCGAAGAACAAGTTTTCGGGTCCGATACCGGAGGAGATTGGGTCGGTGAAGAATCTTATGGAGTTTTCTGGTGGTGACAATAAATTCAGTGGGCCGTTGCCGGAGGGCATTGTGAGACTTGGGCAGCTGGGGAATCTGGACCTTCACAGCAATGAAGTCTCCGGTGAGCTCCCAGTTGGGATCCAATCTTGGACGAAACTAAACGAGCTCAATCTGGCAAGTAACCAACTTTCCGGGAAAATCCCAGATGGAATTGGGAACTTGTCCGTCCTTAATTACCTTGATCTTTCCGGGAATCGATTTTCCGGGAAAATCCCATTTGGGTTGCAGAATATGAAGCTCAATGTTTTTAATTTGTCCTACAATCGGCTCTCAGGTGAGCTTCCTCCCCTTTTTGCTAAGGAAATTTATAGGAATAGCTTTCTGGGGAATCCTGGTCTTTGTGGAGATTTGGATGGGTTGTGTGATAGCAGAGCCGAGGTTAAAAGTCAGGGGTACGTATGGTTGCTTAGGTGCATGTTCATTCTATCTGGTTTAGTATTTGTTGTGGGCGTGGTTTGGTTTTACTTGAAGTACAAGAACTTCAAGAAGGTGAAGCAAACAATAGACAAATCGAAATGGACGCTGATGTCGTTTCACAAACTGGGTTTTAGTGAGTATGAGATCTTGGATTGCCTTGATGAAGATAATGTGATTGGTAGCGGGGCGTCGGGGAAGGTGTACAAGGTTGTGCTGAGCAGTGGAGAGGTTGTTGCAGTAAAGAAGCTATGGAGAGGGAAAGTAAAGGAGTGTGAAGTAGAAAATGTTGAGAAGGTTTGGGTGCGAGACGATGGCTTTGAGGCGGAGGTAGACACTCTGGGGAAGATTAGGCACAAGAACATTGTGAAGCTCTGGTGCTGTTGCACTGCTAGGGACTGCAAGCTCTTGGTTTATGAGTATATGCAAAATGGTAGTCTCGGTGATTTGTTGCATAGCAGCAAAGGAGGGTTGCTGGATTGGCCTACAAGGTTTAAGATAGCTCTAGATGCAGCGGAGGGGCTTTCTTATCTGCACCATGATTGTGTTCCTCCGATTGTTCATAGAGATGTGAAATCAAACAACATATTATTGGACGGGGATTTTGGAGCGCGAGTGGCGGATTTTGGAGTAGCCAAGGAGGTTGATGTAACTGGGAAAGGGCTTAAATCCATGTCCATCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCGTACACTCTCAGAGTGAATGAGAAGAGCGATATATACAGTTTCGGTGTGGTTATTCTGGAGCTGGTGACCGGGAGGCTCCCAGTCGATCCAGAGTTCGGAGAAAACGACTTGGTGAAGTGGGTCTGCACCACATTGGATCAGAAAGGAGTAGACAATGTGGTTGACCCAAAACTTGAGTCTTGTTACAAGGAGGAAGTATGCAAGGTCCTCAACATTGGCCTCCTCTGCACCAGCCCTCTCCCGATTAATCGTCCATCAATGAGACGAGTAGTGAAGTTGTTGCAAGAAGTTGGCACAGAGAAACATCCCCAGGCTGCTAAAAAGGAGGGTAAATTGACACCCTATTACTACGAAGACGTCTCAGATCATGGAAGCGTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

916

Amino Acids

100.86

Weight (kDa)

5.37

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 22 - 62 9.3e-14 Leucine rich repeat N-terminal domain
LRR_14 PF23598 76 - 223 5.7e-11 Leucine-rich repeat region
LRR_8 PF13855 419 - 477 5e-06 Leucine rich repeat
Pkinase PF00069 605 - 831 2.3e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 605 - 880 2.9e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28440
fragaria_vesca FvH4_3g08530 FvH4_3g08530
malus_domestica MD05G1309900.v1.1 MD10G1288500.v1.1
prunus_persica Prupe.4G053100_v2.0.a1
pyrus_communis pycom05g28630
rosa_chinensis RchiOBHm_Chr5g0009311
rosa_laevigata RLG00000031647
rosa_multiflora Rmu_sc0006233.1_g000007
rosa_roxburghii Rroxscaffold_1G00066780 Rroxscaffold_1G00067480
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072200 Rh5BG068700 Rh5CG081100 Rh5DG068900
rosa_wichuraiana Rw5G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1756
AatII GACGTC 1 cut(s) 2724
AbsI CCTCGAGG 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 2321
AccB1I GGYRCC 1 cut(s) 860
AccB7I CCANNNNNTGG 1 cut(s) 967
AccBSI CCGCTC 2 cut(s) 645, 679
AccI GTMKAC 3 cut(s) 267, 1977, 2514
AccII CGCG 4 cut(s) 626, 1036, 1038, 2257
AccIII TCCGGA 1 cut(s) 655
AclWI GGATC 7 cut(s) 358, 371, 931, 1303, 1316, 2444, 2508
AcoI YGGCCR 2 cut(s) 232, 502
AcsI RAATTY 2 cut(s) 1211, 1533
AcyI GRCGYC 4 cut(s) 174, 189, 1835, 2721
AdeI CACNNNGTG 1 cut(s) 417
AfaI GTAC 5 cut(s) 755, 1625, 1703, 1850, 2366
AfiI CCNNNNNNNGG 6 cut(s) 165, 302, 856, 967, 1494, 2459
AflII CTTAAG 1 cut(s) 824
AflIII ACRYGT 1 cut(s) 624
AgsI TTSAA 3 cut(s) 97, 1699, 1714
AjnI CCWGG 4 cut(s) 134, 586, 1561, 2673
Alw21I GWGCWC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
Alw26I GTCTC 8 cut(s) 181, 692, 1237, 1291, 1947, 2087, 2620, 2728
AlwI GGATC 7 cut(s) 358, 371, 931, 1303, 1316, 2444, 2508
Ama87I CYCGRG 5 cut(s) 248, 355, 395, 806, 972
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 8 cut(s) 76, 232, 502, 969, 1076, 1220, 2126, 2581
ApoI RAATTY 2 cut(s) 1211, 1533
ArsI GACNNNNNNTTYG 8 cut(s) 1946, 1978, 2065, 2097, 2231, 2263, 2525, 2557
AseI ATTAAT 2 cut(s) 1121, 2610
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 2 cut(s) 1038, 2257
AspS9I GGNCC 9 cut(s) 400, 478, 970, 976, 1076, 1148, 1220, 1267, 2569
AvaI CYCGRG 5 cut(s) 248, 355, 395, 806, 972
AvaII GGWCC 6 cut(s) 400, 478, 976, 1148, 1267, 2569
BaeGI GKGCMC 1 cut(s) 863
BamHI GGATCC 2 cut(s) 363, 1308
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
BarI GAAGNNNNNNTAC 2 cut(s) 1694, 1726
BbsI GAAGAC 1 cut(s) 2724
Bbv12I GWGCWC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
BccI CCATC 8 cut(s) 296, 494, 1042, 1370, 1577, 1952, 2330, 2626
BceAI ACGGC 3 cut(s) 499, 508, 1207
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 4 cut(s) 136, 588, 1563, 2675
BclI TGATCA 1 cut(s) 531
BcoDI GTCTC 8 cut(s) 181, 692, 1237, 1291, 1947, 2087, 2620, 2728
BfaI CTAG 5 cut(s) 425, 449, 1127, 2037, 2150
BfrI CTTAAG 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 2321
BglII AGATCT 1 cut(s) 1791
BlpI GCTNAGC 3 cut(s) 521, 641, 1862
Bme18I GGWCC 6 cut(s) 400, 478, 976, 1148, 1267, 2569
BmeT110I CYCGRG 5 cut(s) 248, 355, 395, 806, 972
BmgT120I GGNCC 9 cut(s) 400, 478, 970, 976, 1076, 1148, 1220, 1267, 2569
BmiI GGNNCC 7 cut(s) 365, 862, 977, 1102, 1149, 1310, 2441
BmrI ACTGGG 4 cut(s) 1295, 1781, 2307, 2438
BmsI GCATC 2 cut(s) 133, 2143
BmuI ACTGGG 4 cut(s) 1295, 1781, 2307, 2438
BpiI GAAGAC 1 cut(s) 2724
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 2 cut(s) 2337, 2441
Bpu10I CCTNAGC 2 cut(s) 1527, 1637
Bpu1102I GCTNAGC 3 cut(s) 521, 641, 1862
BpuEI CTTGAG 2 cut(s) 452, 2558
Bsa29I ATCGAT 1 cut(s) 1426
BsaAI YACGTR 1 cut(s) 1627
BsaHI GRCGYC 4 cut(s) 174, 189, 1835, 2721
BsaJI CCNNGG 5 cut(s) 355, 972, 1605, 2280, 2673
BsaWI WCCGGW 4 cut(s) 655, 1012, 1156, 1289
Bsc4I CCNNNNNNNGG 6 cut(s) 165, 302, 856, 967, 1494, 2459
Bse118I RCCGGY 4 cut(s) 500, 572, 669, 724
Bse1I ACTGG 5 cut(s) 850, 1301, 1776, 2302, 2444
Bse3DI GCAATG 5 cut(s) 732, 1092, 1285, 2325, 2346
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 4 cut(s) 136, 588, 1563, 2675
BseCI ATCGAT 1 cut(s) 1426
BseDI CCNNGG 5 cut(s) 355, 972, 1605, 2280, 2673
BseGI GGATG 3 cut(s) 288, 1588, 2668
BseLI CCNNNNNNNGG 6 cut(s) 165, 302, 856, 967, 1494, 2459
BseMI GCAATG 5 cut(s) 732, 1092, 1285, 2325, 2346
BseMII CTCAG 7 cut(s) 535, 632, 1050, 1515, 1853, 2386, 2739
BseNI ACTGG 5 cut(s) 850, 1301, 1776, 2302, 2444
BseSI GKGCMC 1 cut(s) 863
BseX3I CGGCCG 2 cut(s) 232, 502
BseYI CCCAGC 1 cut(s) 1255
BsgI GTGCAG 3 cut(s) 2162, 2473, 2575
Bsh1236I CGCG 4 cut(s) 626, 1036, 1038, 2257
Bsh1285I CGRYCG 2 cut(s) 235, 505
BshFI GGCC 8 cut(s) 78, 234, 504, 971, 1078, 1222, 2128, 2583
BshNI GGYRCC 1 cut(s) 860
BshVI ATCGAT 1 cut(s) 1426
BsiEI CGRYCG 2 cut(s) 235, 505
BsiHKAI GWGCWC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
BsiHKCI CYCGRG 5 cut(s) 248, 355, 395, 806, 972
BslFI GGGAC 3 cut(s) 197, 903, 2054
BslI CCNNNNNNNGG 6 cut(s) 165, 302, 856, 967, 1494, 2459
BsmAI GTCTC 8 cut(s) 181, 692, 1237, 1291, 1947, 2087, 2620, 2728
BsmBI CGTCTC 4 cut(s) 181, 1947, 2620, 2728
BsmFI GGGAC 3 cut(s) 197, 903, 2054
BsnI GGCC 8 cut(s) 78, 234, 504, 971, 1078, 1222, 2128, 2583
BsoBI CYCGRG 5 cut(s) 248, 355, 395, 806, 972
Bsp1286I GDGCHC 7 cut(s) 378, 555, 666, 863, 1056, 1299, 1338
Bsp13I TCCGGA 1 cut(s) 655
Bsp1407I TGTACA 1 cut(s) 1848
Bsp143I GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
Bsp1720I GCTNAGC 3 cut(s) 521, 641, 1862
BspANI GGCC 8 cut(s) 78, 234, 504, 971, 1078, 1222, 2128, 2583
BspCNI CTCAG 7 cut(s) 534, 633, 1051, 1514, 1854, 2385, 2738
BspDI ATCGAT 1 cut(s) 1426
BspEI TCCGGA 1 cut(s) 655
BspFNI CGCG 4 cut(s) 626, 1036, 1038, 2257
BspLI GGNNCC 7 cut(s) 365, 862, 977, 1102, 1149, 1310, 2441
BspMI ACCTGC 1 cut(s) 2321
BspPI GGATC 7 cut(s) 358, 371, 931, 1303, 1316, 2444, 2508
BspQI GCTCTTC 3 cut(s) 773, 1082, 2381
BspT107I GGYRCC 1 cut(s) 860
BspTI CTTAAG 1 cut(s) 824
BsrBI CCGCTC 2 cut(s) 645, 679
BsrDI GCAATG 5 cut(s) 732, 1092, 1285, 2325, 2346
BsrFI RCCGGY 4 cut(s) 500, 572, 669, 724
BsrGI TGTACA 1 cut(s) 1848
BsrI ACTGG 5 cut(s) 850, 1301, 1776, 2302, 2444
BssAI RCCGGY 4 cut(s) 500, 572, 669, 724
BssECI CCNNGG 5 cut(s) 355, 972, 1605, 2280, 2673
BssMI GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
BssNI GRCGYC 4 cut(s) 174, 189, 1835, 2721
BssT1I CCWWGG 1 cut(s) 2280
Bst2UI CCWGG 4 cut(s) 136, 588, 1563, 2675
Bst4CI ACNGT 1 cut(s) 2402
Bst6I CTCTTC 5 cut(s) 14, 773, 894, 1082, 2381
BstACI GRCGYC 4 cut(s) 174, 189, 1835, 2721
BstAFI CTTAAG 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 1848
BstBAI YACGTR 1 cut(s) 1627
BstC8I GCNNGC 9 cut(s) 527, 578, 647, 722, 726, 730, 791, 1056, 2048
BstEII GGTNACC 1 cut(s) 2428
BstF5I GGATG 3 cut(s) 288, 1588, 2668
BstFNI CGCG 4 cut(s) 626, 1036, 1038, 2257
BstHHI GCGC 2 cut(s) 1038, 2257
BstKTI GATC 9 cut(s) 366, 534, 939, 1311, 1414, 1794, 2452, 2503, 2731
BstMAI GTCTC 8 cut(s) 181, 692, 1237, 1291, 1947, 2087, 2620, 2728
BstMBI GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
BstMCI CGRYCG 2 cut(s) 235, 505
BstNI CCWGG 4 cut(s) 136, 588, 1563, 2675
BstNSI RCATGY 1 cut(s) 1648
BstPI GGTNACC 1 cut(s) 2428
BstSLI GKGCMC 1 cut(s) 863
BstSNI TACGTA 1 cut(s) 1627
BstUI CGCG 4 cut(s) 626, 1036, 1038, 2257
BstV2I GAAGAC 1 cut(s) 2724
BstX2I RGATCY 4 cut(s) 363, 936, 1308, 1791
BstYI RGATCY 4 cut(s) 363, 936, 1308, 1791
BstZI CGGCCG 2 cut(s) 232, 502
Bsu15I ATCGAT 1 cut(s) 1426
BsuRI GGCC 8 cut(s) 78, 234, 504, 971, 1078, 1222, 2128, 2583
BsuTUI ATCGAT 1 cut(s) 1426
BtsCI GGATG 3 cut(s) 288, 1588, 2668
BtsI GCAGTG 2 cut(s) 1873, 2031
BtsIMutI CAGTG 3 cut(s) 1222, 1873, 2031
BveI ACCTGC 1 cut(s) 2321
Cac8I GCNNGC 9 cut(s) 527, 578, 647, 722, 726, 730, 791, 1056, 2048
CfoI GCGC 2 cut(s) 1038, 2257
Cfr10I RCCGGY 4 cut(s) 500, 572, 669, 724
Cfr13I GGNCC 9 cut(s) 400, 478, 970, 976, 1076, 1148, 1220, 1267, 2569
Cfr9I CCCGGG 2 cut(s) 355, 972
ClaI ATCGAT 1 cut(s) 1426
CseI GACGC 7 cut(s) 163, 197, 526, 615, 632, 1759, 1824
Csp6I GTAC 5 cut(s) 754, 1624, 1702, 1849, 2365
CviAII CATG 6 cut(s) 180, 631, 1645, 2183, 2317, 2732
CviQI GTAC 5 cut(s) 754, 1624, 1702, 1849, 2365
DpnI GATC 9 cut(s) 365, 533, 938, 1310, 1413, 1793, 2451, 2502, 2730
DpnII GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
DraIII CACNNNGTG 1 cut(s) 417
DrdI GACNNNNNNGTC 1 cut(s) 1756
DseDI GACNNNNNNGTC 1 cut(s) 1756
EaeI YGGCCR 2 cut(s) 232, 502
EagI CGGCCG 2 cut(s) 232, 502
Eam1104I CTCTTC 5 cut(s) 14, 773, 894, 1082, 2381
EarI CTCTTC 5 cut(s) 14, 773, 894, 1082, 2381
EciI GGCGGA 5 cut(s) 112, 386, 501, 1985, 2279
Ecl136II GAGCTC 6 cut(s) 376, 553, 664, 1054, 1297, 1336
EclXI CGGCCG 2 cut(s) 232, 502
Eco105I TACGTA 1 cut(s) 1627
Eco130I CCWWGG 1 cut(s) 2280
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
Eco47I GGWCC 6 cut(s) 400, 478, 976, 1148, 1267, 2569
Eco52I CGGCCG 2 cut(s) 232, 502
Eco53kI GAGCTC 6 cut(s) 376, 553, 664, 1054, 1297, 1336
Eco88I CYCGRG 5 cut(s) 248, 355, 395, 806, 972
Eco91I GGTNACC 1 cut(s) 2428
EcoICRI GAGCTC 6 cut(s) 376, 553, 664, 1054, 1297, 1336
EcoO109I RGGNCCY 2 cut(s) 400, 2569
EcoO65I GGTNACC 1 cut(s) 2428
EcoRII CCWGG 4 cut(s) 134, 586, 1561, 2673
EcoT14I CCWWGG 1 cut(s) 2280
EcoT38I GRGCYC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
ErhI CCWWGG 1 cut(s) 2280
Esp3I CGTCTC 4 cut(s) 181, 1947, 2620, 2728
FaeI CATG 6 cut(s) 183, 634, 1648, 2186, 2320, 2735
FalI AAGNNNNNCTT 1 cut(s) 2728
FaqI GGGAC 3 cut(s) 197, 903, 2054
FatI CATG 6 cut(s) 179, 630, 1644, 2182, 2316, 2731
FauI CCCGC 4 cut(s) 58, 300, 638, 1823
FbaI TGATCA 1 cut(s) 531
FblI GTMKAC 3 cut(s) 267, 1977, 2514
FokI GGATG 3 cut(s) 275, 1595, 2655
FriOI GRGCYC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
FspBI CTAG 5 cut(s) 425, 449, 1127, 2037, 2150
GlaI GCGC 2 cut(s) 1037, 2256
GsaI CCCAGC 1 cut(s) 1259
GsuI CTGGAG 2 cut(s) 2337, 2441
HaeIII GGCC 8 cut(s) 78, 234, 504, 971, 1078, 1222, 2128, 2583
HgaI GACGC 7 cut(s) 163, 197, 526, 615, 632, 1759, 1824
HhaI GCGC 2 cut(s) 1038, 2257
Hin1I GRCGYC 4 cut(s) 174, 189, 1835, 2721
Hin1II CATG 6 cut(s) 183, 634, 1648, 2186, 2320, 2735
Hin6I GCGC 2 cut(s) 1036, 2255
HinP1I GCGC 2 cut(s) 1036, 2255
HincII GTYRAC 2 cut(s) 455, 2527
HindII GTYRAC 2 cut(s) 455, 2527
Hpy166II GTNNAC 9 cut(s) 268, 455, 855, 1042, 1849, 1978, 2367, 2515, 2527
Hpy188I TCNGA 9 cut(s) 363, 773, 980, 1152, 2199, 2375, 2462, 2505, 2728
Hpy8I GTNNAC 9 cut(s) 268, 455, 855, 1042, 1849, 1978, 2367, 2515, 2527
Hpy99I CGWCG 7 cut(s) 113, 188, 191, 520, 542, 626, 1840
HpyAV CCTTC 7 cut(s) 68, 238, 880, 1280, 1711, 1837, 1933
HpyCH4III ACNGT 1 cut(s) 2402
HpyCH4IV ACGT 2 cut(s) 1626, 2721
HpySE526I ACGT 2 cut(s) 1626, 2721
Hsp92I GRCGYC 4 cut(s) 174, 189, 1835, 2721
Hsp92II CATG 6 cut(s) 183, 634, 1648, 2186, 2320, 2735
HspAI GCGC 2 cut(s) 1036, 2255
Kpn2I TCCGGA 1 cut(s) 655
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
Ksp22I TGATCA 1 cut(s) 531
Kzo9I GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
LguI GCTCTTC 3 cut(s) 773, 1082, 2381
LmnI GCTCC 9 cut(s) 373, 717, 1051, 1100, 1302, 2252, 2356, 2422, 2445
LweI GCATC 2 cut(s) 133, 2143
MaeI CTAG 5 cut(s) 425, 449, 1127, 2037, 2150
MaeII ACGT 2 cut(s) 1626, 2721
MaeIII GTNAC 7 cut(s) 986, 1202, 1349, 2293, 2342, 2428, 2546
MalI GATC 9 cut(s) 365, 533, 938, 1310, 1413, 1793, 2451, 2502, 2730
MbiI CCGCTC 2 cut(s) 645, 679
MboI GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 4 cut(s) 363, 936, 1308, 1791
MhlI GDGCHC 7 cut(s) 378, 555, 666, 863, 1056, 1299, 1338
MluI ACGCGT 1 cut(s) 624
MlyI GAGTC 6 cut(s) 113, 143, 218, 437, 883, 2549
MmeI TCCRAC 2 cut(s) 112, 341
MroI TCCGGA 1 cut(s) 655
MroNI GCCGGC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 903
MslI CAYNNNNRTG 3 cut(s) 1278, 2187, 2211
MspA1I CMGCKG 3 cut(s) 640, 1255, 2159
MspCI CTTAAG 1 cut(s) 824
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 4 cut(s) 136, 588, 1563, 2675
MvnI CGCG 4 cut(s) 626, 1036, 1038, 2257
NaeI GCCGGC 1 cut(s) 726
NdeII GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
NgoMIV GCCGGC 1 cut(s) 724
NlaIII CATG 6 cut(s) 183, 634, 1648, 2186, 2320, 2735
NlaIV GGNNCC 7 cut(s) 365, 862, 977, 1102, 1149, 1310, 2441
NmeAIII GCCGAG 3 cut(s) 282, 357, 1630
NmuCI GTSAC 3 cut(s) 986, 1202, 2428
NspI RCATGY 1 cut(s) 1648
PaeR7I CTCGAG 2 cut(s) 248, 395
PceI AGGCCT 1 cut(s) 78
PciSI GCTCTTC 3 cut(s) 773, 1082, 2381
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 1757, 2466
PdiI GCCGGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 8 cut(s) 436, 508, 652, 904, 1180, 1261, 1423, 1558
PflMI CCANNNNNTGG 1 cut(s) 967
PfoI TCCNGGA 4 cut(s) 134, 1361, 1418, 1433
PleI GAGTC 6 cut(s) 113, 143, 218, 437, 882, 2548
PpsI GAGTC 6 cut(s) 113, 143, 218, 437, 882, 2548
Ppu21I YACGTR 1 cut(s) 1627
PpuMI RGGWCCY 2 cut(s) 400, 2569
PshBI ATTAAT 2 cut(s) 1121, 2610
Psp124BI GAGCTC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
Psp5II RGGWCCY 2 cut(s) 400, 2569
Psp6I CCWGG 4 cut(s) 134, 586, 1561, 2673
PspEI GGTNACC 1 cut(s) 2428
PspFI CCCAGC 1 cut(s) 1255
PspGI CCWGG 4 cut(s) 134, 586, 1561, 2673
PspN4I GGNNCC 7 cut(s) 365, 862, 977, 1102, 1149, 1310, 2441
PspPI GGNCC 9 cut(s) 400, 478, 970, 976, 1076, 1148, 1220, 1267, 2569
PspPPI RGGWCCY 2 cut(s) 400, 2569
PspXI VCTCGAGB 2 cut(s) 248, 395
PsuI RGATCY 4 cut(s) 363, 936, 1308, 1791
PvuII CAGCTG 2 cut(s) 640, 1255
RsaI GTAC 5 cut(s) 755, 1625, 1703, 1850, 2366
RsaNI GTAC 5 cut(s) 754, 1624, 1702, 1849, 2365
RseI CAYNNNNRTG 3 cut(s) 1278, 2187, 2211
SacI GAGCTC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
SapI GCTCTTC 3 cut(s) 773, 1082, 2381
Sau3AI GATC 9 cut(s) 363, 531, 936, 1308, 1411, 1791, 2449, 2500, 2728
Sau96I GGNCC 9 cut(s) 400, 478, 970, 976, 1076, 1148, 1220, 1267, 2569
SchI GAGTC 6 cut(s) 113, 143, 218, 437, 883, 2549
SduI GDGCHC 7 cut(s) 378, 555, 666, 863, 1056, 1299, 1338
SfaNI GCATC 2 cut(s) 133, 2143
Sfr274I CTCGAG 2 cut(s) 248, 395
SinI GGWCC 6 cut(s) 400, 478, 976, 1148, 1267, 2569
SlaI CTCGAG 2 cut(s) 248, 395
SmaI CCCGGG 2 cut(s) 357, 974
SmiMI CAYNNNNRTG 3 cut(s) 1278, 2187, 2211
SmlI CTYRAG 5 cut(s) 248, 395, 467, 824, 2537
SmoI CTYRAG 5 cut(s) 248, 395, 467, 824, 2537
SnaBI TACGTA 1 cut(s) 1627
SseBI AGGCCT 1 cut(s) 78
SspMI CTAG 5 cut(s) 425, 449, 1127, 2037, 2150
SstI GAGCTC 6 cut(s) 378, 555, 666, 1056, 1299, 1338
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 1 cut(s) 2280
TaaI ACNGT 1 cut(s) 2402
TaiI ACGT 2 cut(s) 1629, 2724
TatI WGTACW 2 cut(s) 1701, 1848
TauI GCSGC 6 cut(s) 43, 237, 586, 679, 823, 1036
TfiI GAWTC 8 cut(s) 436, 508, 652, 904, 1180, 1261, 1423, 1558
TscAI CASTG 3 cut(s) 1222, 1873, 2038
TseFI GTSAC 3 cut(s) 986, 1202, 2428
Tsp45I GTSAC 3 cut(s) 986, 1202, 2428
TspDTI ATGAA 6 cut(s) 647, 1296, 1478, 1639, 1824, 2195
TspGWI ACGGA 2 cut(s) 196, 1384
TspMI CCCGGG 2 cut(s) 355, 972
TspRI CASTG 3 cut(s) 1222, 1873, 2038
Van91I CCANNNNNTGG 1 cut(s) 967
Vha464I CTTAAG 1 cut(s) 824
VpaK11BI GGWCC 6 cut(s) 400, 478, 976, 1148, 1267, 2569
VspI ATTAAT 2 cut(s) 1121, 2610
XapI RAATTY 2 cut(s) 1211, 1533
XbaI TCTAGA 1 cut(s) 2149
XceI RCATGY 1 cut(s) 1648
XhoI CTCGAG 2 cut(s) 248, 395
XmaI CCCGGG 2 cut(s) 355, 972
XmiI GTMKAC 3 cut(s) 267, 1977, 2514
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 5 cut(s) 425, 449, 1127, 2037, 2150
ZraI GACGTC 1 cut(s) 2722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.