pycom05g31460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018130
Physical Location & Seq
Reverse (-)
31193969 .. 31195074
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31460.1

Sequence Viewer

Length: 444 bp
ATGCCCCCCCTGTGCTGCGTTGGGGACTTTCTTGATTCAAAAACCAGCTGTGATCTGTTGCAAGAAAGACTCAGAAAAGAAAATACCTCTACATACCGAAGATTATGTCCAACTTTAGCATGCTCGAAACCGCGGAATCACTGCCACCAACAGCTATGGCTGCGCAGTTCGTCATCGTCCAGTATTCCTCCGAAATTCAACTCTCTTTACTCTCTCCGACTCAGTGCGTCTCTATTCTTCCAGGTACACCCATATCTATATCCCAAACGACTCTTTATATGTATCTATTTGTTAGAGAAGGCTTTTGCCCTTTGGGTTCAATTTTATCTGCTGTCTGCGTTGGATTTGATGTCTTCTCAGTCCTTATTTTTTTATCCAGCAATGGAGCTTGCATTATCACTTGAGAAATTGGCAAATGAAAGCTGCTTCACTTGCAGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.04

Weight (kDa)

8.9

Isoelectric Point (pI)

68.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 164
AccII CGCG 1 cut(s) 133
AciI CCGC 2 cut(s) 131, 133
AcsI RAATTY 1 cut(s) 194
AfaI GTAC 1 cut(s) 246
AgsI TTSAA 3 cut(s) 39, 199, 320
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 4 cut(s) 48, 154, 388, 423
AluI AGCT 4 cut(s) 48, 154, 388, 423
Alw26I GTCTC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 15, 160, 423
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
AspLEI GCGC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 2, 147, 410
BciT130I CCWGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 234
BisI GCNGC 3 cut(s) 16, 161, 424
BlsI GCNGC 3 cut(s) 17, 162, 425
Bme1390I CCNGG 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 422
BsaJI CCNNGG 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 3 cut(s) 85, 235, 371
BseNI ACTGG 1 cut(s) 180
BseXI GCAGC 3 cut(s) 2, 147, 410
Bsh1236I CGCG 1 cut(s) 133
BslFI GGGAC 1 cut(s) 38
BsmAI GTCTC 1 cut(s) 234
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 38
Bsp143I GATC 1 cut(s) 52
BspACI CCGC 2 cut(s) 131, 133
BspCNI CTCAG 3 cut(s) 84, 234, 370
BspFNI CGCG 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 121, 390
BstDEI CTNAG 3 cut(s) 71, 221, 357
BstDSI CCRYGG 1 cut(s) 131
BstFNI CGCG 1 cut(s) 133
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 160, 432
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 240
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 3 cut(s) 2, 147, 410
BstV2I GAAGAC 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 131
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 2 cut(s) 139, 229
Cac8I GCNNGC 2 cut(s) 121, 390
CfoI GCGC 1 cut(s) 165
Cfr42I CCGCGG 1 cut(s) 134
CseI GACGC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 245
CviAII CATG 1 cut(s) 120
CviJI RGCY 6 cut(s) 48, 154, 160, 302, 388, 423
CviKI_1 RGCY 6 cut(s) 48, 154, 160, 302, 388, 423
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 3 cut(s) 71, 221, 357
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 1 cut(s) 123
FaiI YATR 8 cut(s) 94, 106, 121, 157, 253, 259, 278, 280
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 119
Fnu4HI GCNGC 3 cut(s) 16, 161, 424
Fsp4HI GCNGC 3 cut(s) 16, 161, 424
FspI TGCGCA 1 cut(s) 164
GlaI GCGC 1 cut(s) 164
GluI GCNGC 3 cut(s) 16, 161, 424
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 165
Hin1II CATG 1 cut(s) 123
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 5 cut(s) 35, 69, 136, 219, 270
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 3 cut(s) 74, 192, 218
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 292
HpyCH4V TGCA 3 cut(s) 61, 392, 435
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 432
HpyF3I CTNAG 3 cut(s) 71, 221, 357
Hsp92II CATG 1 cut(s) 123
HspAI GCGC 1 cut(s) 163
KspI CCGCGG 1 cut(s) 134
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 385
LpnPI CCDG 6 cut(s) 23, 58, 193, 227, 254, 390
Lsp1109I GCAGC 3 cut(s) 2, 147, 410
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 3 cut(s) 111, 229, 345
MluCI AATT 3 cut(s) 194, 320, 407
MlyI GAGTC 3 cut(s) 63, 213, 264
MmeI TCCRAC 3 cut(s) 134, 241, 321
MnlI CCTC 2 cut(s) 97, 198
MspA1I CMGCKG 2 cut(s) 48, 133
MspR9I CCNGG 1 cut(s) 242
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 160, 432
NdeII GATC 1 cut(s) 52
NlaIII CATG 1 cut(s) 123
NsbI TGCGCA 1 cut(s) 164
NspI RCATGY 1 cut(s) 123
PaeI GCATGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 35, 136
PkrI GCNGC 3 cut(s) 17, 162, 425
PleI GAGTC 3 cut(s) 63, 213, 264
PpsI GAGTC 3 cut(s) 63, 213, 264
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 48
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SacII CCGCGG 1 cut(s) 134
SatI GCNGC 3 cut(s) 16, 161, 424
Sau3AI GATC 1 cut(s) 52
SchI GAGTC 3 cut(s) 63, 213, 264
ScrFI CCNGG 1 cut(s) 242
SetI ASST 6 cut(s) 50, 89, 156, 246, 390, 425
Sfr303I CCGCGG 1 cut(s) 134
SgrBI CCGCGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SphI GCATGC 1 cut(s) 123
Sse9I AATT 3 cut(s) 194, 320, 407
SsiI CCGC 2 cut(s) 131, 133
StyD4I CCNGG 1 cut(s) 240
TaqI TCGA 1 cut(s) 125
TasI AATT 3 cut(s) 194, 320, 407
TfiI GAWTC 2 cut(s) 35, 136
TscAI CASTG 2 cut(s) 146, 229
TseI GCWGC 3 cut(s) 15, 160, 423
TspDTI ATGAA 1 cut(s) 432
TspRI CASTG 2 cut(s) 146, 229
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.