pycom05g31590

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009782
Physical Location & Seq
Forward (+)
31293951 .. 31297026
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31590.3

Sequence Viewer

Length: 1053 bp
ATGAGAGACTGCAAGCTTCTTTCTTTTGCCATTTTTTTGGTTCTTTTTCTTGTTAATGGCGTTATTGGTGAAGACCCGTATAGATTTTTCACTTGGAAAGTGACTTATGGCGACATTTACCCTCTTGGTGTTAAGCAACGGCGATCTTTGATCAATGGGCAATTTCCAGGGCCTCAGATCGATGCTGTTACAAACGATAACTTGGTCATTAGCGTCTTCAACTACCTGAGGGAACCATTCCTCATTTCTTGGGATGGCATACAGCAGAGAAGGAACTCATGGCAGGACGGAGTTTTGGGAACAAACTGTCCAATCCCGCCGGGGAGGAGGTTTACTTACAATATCCAAGTCAAGGATCAGATTGGTAGCTTTTTCTACTTTCCCTCCCTCCAGTTCCACAAAGCCGCCGGAGGTTTTGGTGGCATCAGAATCCGGAGCCGTCCTAAGATTCCCGTTCCTTTCCCTAATCCTGCCGGAGACTTCACCGTGCTGGCCGGGGACTGGTTCAAGACTAATCATTATTTACTGAGACGAGGCTACTTGGATAGAGGTCGAGCTCTTCCCAACCCTGACGGGCTTCTCATCAACGGTCGTGGATGGAACGGATATACATTCACCGTTGATCAAGGCAAGACATATAGGTTTAGGATATCAAACGTGGGGCTTACGACATCCATTAACTTCAGAATTCAAGGACACAAAATGAAACTTGTTGAGGTGGAAGGATCTCATACACTGCAGAACATGTATGACTCCCTTGACATCCATCTCGGACAGTCCTTATCCGTCTTGGTCACAGCTAATCAGCCTGTCCAGGACTACTACGTCGTTGTGTCTTCACGCTTCACCCGCCGGGTACTCACCACAACAGCCGTTCTTCACTATAGCAATTCGCGTAAGGGAGTGTCGGGTCCTATCCCTTGGGGTCCTACCACACAGATTGCTTGGTCTGTAAGCCAAGCGAGATCTATAAGATGGAATTTGACAGCGAGCGGGCCAAGGCCTAATCCGCAAGGCTCCTACCACTACGGATTGATCAAGCTTCCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

351

Amino Acids

39.58

Weight (kDa)

10.11

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 824
AccBSI CCGCTC 1 cut(s) 993
AccII CGCG 1 cut(s) 895
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 5 cut(s) 317, 405, 850, 993, 1010
AclWI GGATC 2 cut(s) 363, 733
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 2 cut(s) 687, 979
AcuI CTGAAG 1 cut(s) 667
AfaI GTAC 1 cut(s) 858
AfiI CCNNNNNNNGG 2 cut(s) 352, 501
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 3 cut(s) 220, 508, 692
AjnI CCWGG 2 cut(s) 166, 813
AloI GAACNNNNNNTCC 2 cut(s) 292, 324
AluBI AGCT 6 cut(s) 16, 369, 557, 800, 1042, 1050
AluI AGCT 6 cut(s) 16, 369, 557, 800, 1042, 1050
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 2 cut(s) 471, 523
AlwI GGATC 2 cut(s) 363, 733
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 4 cut(s) 170, 492, 995, 1001
ApoI RAATTY 2 cut(s) 687, 979
AspS9I GGNCC 4 cut(s) 170, 911, 926, 995
AsuC2I CCSGG 3 cut(s) 321, 496, 854
AsuHPI GGTGA 5 cut(s) 80, 475, 607, 838, 853
AvaII GGWCC 2 cut(s) 911, 926
AxyI CCTNAGG 1 cut(s) 227
BanII GRGCYC 1 cut(s) 559
BbsI GAAGAC 3 cut(s) 78, 208, 828
Bbv12I GWGCWC 1 cut(s) 559
BccI CCATC 4 cut(s) 248, 591, 774, 969
BceAI ACGGC 3 cut(s) 155, 423, 857
BciT130I CCWGG 2 cut(s) 168, 815
BclI TGATCA 3 cut(s) 150, 622, 1035
BcnI CCSGG 3 cut(s) 321, 496, 854
BcoDI GTCTC 2 cut(s) 471, 523
BfaI CTAG 1 cut(s) 1051
BfmI CTRYAG 2 cut(s) 737, 883
BglII AGATCT 1 cut(s) 965
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 5 cut(s) 168, 321, 496, 815, 854
Bme18I GGWCC 2 cut(s) 911, 926
BmgT120I GGNCC 4 cut(s) 170, 911, 926, 995
BmiI GGNNCC 5 cut(s) 234, 437, 912, 927, 1018
BmrFI CCNGG 5 cut(s) 168, 321, 496, 815, 854
BmsI GCATC 2 cut(s) 172, 432
BpiI GAAGAC 3 cut(s) 78, 208, 828
BpmI CTGGAG 1 cut(s) 374
BpuMI CCSGG 3 cut(s) 321, 496, 854
Bsa29I ATCGAT 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 167, 320, 495, 920, 998
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 501
Bse1I ACTGG 2 cut(s) 391, 506
Bse21I CCTNAGG 1 cut(s) 227
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 2 cut(s) 168, 815
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 5 cut(s) 167, 320, 495, 920, 998
BseGI GGATG 4 cut(s) 259, 602, 671, 762
BseLI CCNNNNNNNGG 2 cut(s) 352, 501
BseMII CTCAG 3 cut(s) 188, 218, 518
BseNI ACTGG 2 cut(s) 391, 506
BseRI GAGGAG 1 cut(s) 340
BseYI CCCAGC 1 cut(s) 1046
Bsh1236I CGCG 1 cut(s) 895
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 4 cut(s) 172, 494, 997, 1003
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 6 cut(s) 320, 408, 433, 474, 495, 853
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 2 cut(s) 352, 501
BsmAI GTCTC 2 cut(s) 471, 523
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 4 cut(s) 172, 494, 997, 1003
Bsp1286I GDGCHC 1 cut(s) 559
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
BspACI CCGC 5 cut(s) 317, 405, 850, 993, 1010
BspANI GGCC 4 cut(s) 172, 494, 997, 1003
BspCNI CTCAG 3 cut(s) 187, 219, 519
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 1 cut(s) 895
BspLI GGNNCC 5 cut(s) 234, 437, 912, 927, 1018
BspMAI CTGCAG 1 cut(s) 741
BspPI GGATC 2 cut(s) 363, 733
BspQI GCTCTTC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 993
BsrI ACTGG 2 cut(s) 391, 506
BssECI CCNNGG 5 cut(s) 167, 320, 495, 920, 998
BssMI GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
BssT1I CCWWGG 2 cut(s) 920, 998
Bst2UI CCWGG 2 cut(s) 168, 815
Bst4CI ACNGT 5 cut(s) 308, 487, 590, 619, 777
Bst6I CTCTTC 1 cut(s) 564
BstC8I GCNNGC 4 cut(s) 14, 492, 991, 995
BstDEI CTNAG 4 cut(s) 174, 227, 444, 527
BstF5I GGATG 4 cut(s) 259, 602, 671, 762
BstFNI CGCG 1 cut(s) 895
BstKTI GATC 8 cut(s) 146, 153, 180, 358, 625, 728, 968, 1038
BstMAI GTCTC 2 cut(s) 471, 523
BstMBI GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 2 cut(s) 849, 1009
BstNI CCWGG 2 cut(s) 168, 815
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 5 cut(s) 166, 319, 494, 813, 852
BstSFI CTRYAG 2 cut(s) 737, 883
BstUI CGCG 1 cut(s) 895
BstV2I GAAGAC 3 cut(s) 78, 208, 828
BstX2I RGATCY 2 cut(s) 725, 965
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 2 cut(s) 725, 965
Bsu15I ATCGAT 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 4 cut(s) 172, 494, 997, 1003
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 4 cut(s) 259, 602, 671, 762
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 1 cut(s) 734
Cac8I GCNNGC 4 cut(s) 14, 492, 991, 995
Cfr13I GGNCC 4 cut(s) 170, 911, 926, 995
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 857
CspCI CAANNNNNGTGG 4 cut(s) 574, 609, 922, 957
CviAII CATG 2 cut(s) 279, 745
CviQI GTAC 1 cut(s) 857
DdeI CTNAG 4 cut(s) 174, 227, 444, 527
DpnI GATC 8 cut(s) 145, 152, 179, 357, 624, 727, 967, 1037
DpnII GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 1 cut(s) 492
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 920, 998
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 559
Eco32I GATATC 1 cut(s) 651
Eco47I GGWCC 2 cut(s) 911, 926
Eco53kI GAGCTC 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 667
Eco81I CCTNAGG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 557
EcoO109I RGGNCCY 3 cut(s) 170, 911, 926
EcoRI GAATTC 1 cut(s) 687
EcoRII CCWGG 2 cut(s) 166, 813
EcoRV GATATC 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 920, 998
EcoT38I GRGCYC 1 cut(s) 559
ErhI CCWWGG 2 cut(s) 920, 998
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 2 cut(s) 282, 748
FaqI GGGAC 1 cut(s) 512
FatI CATG 2 cut(s) 278, 744
FauI CCCGC 3 cut(s) 324, 857, 986
FbaI TGATCA 3 cut(s) 150, 622, 1035
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 4 cut(s) 266, 609, 658, 749
FriOI GRGCYC 1 cut(s) 559
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 1 cut(s) 1051
GluI GCNGC 1 cut(s) 405
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 4 cut(s) 172, 494, 997, 1003
HapII CCGG 6 cut(s) 320, 408, 433, 474, 495, 853
HgaI GACGC 1 cut(s) 202
Hin1II CATG 2 cut(s) 282, 748
HindIII AAGCTT 2 cut(s) 14, 1040
HinfI GANTC 3 cut(s) 429, 448, 752
HpaII CCGG 6 cut(s) 320, 408, 433, 474, 495, 853
HphI GGTGA 5 cut(s) 80, 475, 607, 838, 853
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 5 cut(s) 177, 360, 428, 686, 773
Hpy188III TCNNGA 2 cut(s) 433, 508
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 2 cut(s) 264, 716
HpyCH4III ACNGT 5 cut(s) 308, 487, 590, 619, 777
HpyCH4IV ACGT 2 cut(s) 657, 825
HpyCH4V TGCA 2 cut(s) 12, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 849, 1009
HpyF3I CTNAG 4 cut(s) 174, 227, 444, 527
HpySE526I ACGT 2 cut(s) 657, 825
Hsp92II CATG 2 cut(s) 282, 748
Kpn2I TCCGGA 1 cut(s) 432
Ksp22I TGATCA 3 cut(s) 150, 622, 1035
Kzo9I GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
LguI GCTCTTC 1 cut(s) 564
LmnI GCTCC 2 cut(s) 435, 1022
LweI GCATC 2 cut(s) 172, 432
MaeI CTAG 1 cut(s) 1051
MaeII ACGT 2 cut(s) 657, 825
MaeIII GTNAC 3 cut(s) 100, 187, 793
MalI GATC 8 cut(s) 145, 152, 179, 357, 624, 727, 967, 1037
MbiI CCGCTC 1 cut(s) 993
MboI GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
MboII GAAGA 5 cut(s) 83, 208, 551, 828, 869
MflI RGATCY 2 cut(s) 725, 965
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 4 cut(s) 161, 687, 889, 979
MlyI GAGTC 1 cut(s) 746
MroI TCCGGA 1 cut(s) 432
MseI TTAA 3 cut(s) 54, 132, 678
MspI CCGG 6 cut(s) 320, 408, 433, 474, 495, 853
MspR9I CCNGG 5 cut(s) 168, 321, 496, 815, 854
MvaI CCWGG 2 cut(s) 168, 815
MvnI CGCG 1 cut(s) 895
MwoI GCNNNNNNNGC 2 cut(s) 849, 1009
NciI CCSGG 3 cut(s) 321, 496, 854
NdeII GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
NlaIII CATG 2 cut(s) 282, 748
NlaIV GGNNCC 5 cut(s) 234, 437, 912, 927, 1018
NmuCI GTSAC 2 cut(s) 100, 793
NspI RCATGY 1 cut(s) 748
PceI AGGCCT 1 cut(s) 1003
PciI ACATGT 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 564
PfeI GAWTC 2 cut(s) 429, 448
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 1 cut(s) 406
PleI GAGTC 1 cut(s) 746
PpsI GAGTC 1 cut(s) 746
PpuMI RGGWCCY 2 cut(s) 911, 926
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 559
Psp5II RGGWCCY 2 cut(s) 911, 926
Psp6I CCWGG 2 cut(s) 166, 813
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 2 cut(s) 166, 813
PspN4I GGNNCC 5 cut(s) 234, 437, 912, 927, 1018
PspPI GGNCC 4 cut(s) 170, 911, 926, 995
PspPPI RGGWCCY 2 cut(s) 911, 926
PstI CTGCAG 1 cut(s) 741
PsuI RGATCY 2 cut(s) 725, 965
RsaI GTAC 1 cut(s) 858
RsaNI GTAC 1 cut(s) 857
SacI GAGCTC 1 cut(s) 559
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 3 cut(s) 54, 132, 678
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 8 cut(s) 143, 150, 177, 355, 622, 725, 965, 1035
Sau96I GGNCC 4 cut(s) 170, 911, 926, 995
SchI GAGTC 1 cut(s) 746
ScrFI CCNGG 5 cut(s) 168, 321, 496, 815, 854
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 2 cut(s) 172, 432
SfcI CTRYAG 2 cut(s) 737, 883
SinI GGWCC 2 cut(s) 911, 926
Sse9I AATT 4 cut(s) 161, 687, 889, 979
SseBI AGGCCT 1 cut(s) 1003
SsiI CCGC 5 cut(s) 317, 405, 850, 993, 1010
SspMI CTAG 1 cut(s) 1051
SstI GAGCTC 1 cut(s) 559
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 5 cut(s) 166, 319, 494, 813, 852
StyI CCWWGG 2 cut(s) 920, 998
TaaI ACNGT 5 cut(s) 308, 487, 590, 619, 777
TaiI ACGT 2 cut(s) 660, 828
TaqI TCGA 2 cut(s) 180, 553
TasI AATT 4 cut(s) 161, 687, 889, 979
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 2 cut(s) 429, 448
Tru1I TTAA 3 cut(s) 54, 132, 678
Tru9I TTAA 3 cut(s) 54, 132, 678
TscAI CASTG 1 cut(s) 741
TseFI GTSAC 2 cut(s) 100, 793
Tsp45I GTSAC 2 cut(s) 100, 793
TspDTI ATGAA 1 cut(s) 719
TspGWI ACGGA 4 cut(s) 303, 618, 775, 1044
TspRI CASTG 1 cut(s) 741
VpaK11BI GGWCC 2 cut(s) 911, 926
XapI RAATTY 2 cut(s) 687, 979
XceI RCATGY 1 cut(s) 748
XspI CTAG 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.