Rh5BG024700

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009782
Physical Location & Seq
Reverse (-)
1749811 .. 1753020
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG024700.1

Sequence Viewer

Length: 675 bp
ATGAGAGAGTGTAAGATTTATTGTTTTTGCATTGCTTTGGTGCTGGTTTTAGTTCATGGCGCTAACGGTGAAGACCCATATAAGTTAATCACCTGGAAAATCACTTACGGCGACATTTATCCTCTTGGTGTTAAGCAACAGGGGATCTTGATCAATGACCAATTTCCAGGGCCTCAAATTGATGCTGTGACAAATATGAACTTGGTTATCAGTGTGTTCAATTACTTGAGTGAACCATTTCTTATTTCTTGGGATGGTATACAGCAGAGAAGGAACTCATGGCAGGATGGAATGTATGGCACTAACTGTCCAATTCTACCTGGAAAGAGCTTCACTTACAATATCCAAGTGAAGGATCAGATTGGTAGCTTTTTCTACTTCCCCTCATTTTTGTTCCACAAAGCTGCTGGAGGCTTTGGTGGCATCAGAATCTGGAGCCGTCCTAAGATTCCGGTTCCTTTCCCTAATCCTGCTGGAGACCACACTGTACTAGCCGGCGACTGGTTTAAAACTAATCATTATATACTGAGACGATACTTCTTGGAGCGTGGTCGCAATCTTCCCTTGCCTGATGGTCTTCTCATCAACGGTCGTGGATGGAATGGATACACATTCACTGTTGAACCAGGTAGGTGTCTAAAAAACAACTTCTTTATATCCCACCCTCAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

224

Amino Acids

25.6

Weight (kDa)

9.19

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 34 - 147 1e-37 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 259
AclWI GGATC 2 cut(s) 152, 363
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 2 cut(s) 352, 501
AgsI TTSAA 2 cut(s) 220, 623
AjnI CCWGG 4 cut(s) 92, 166, 319, 625
AluBI AGCT 3 cut(s) 330, 369, 404
AluI AGCT 3 cut(s) 330, 369, 404
Alw26I GTCTC 2 cut(s) 471, 523
AlwI GGATC 2 cut(s) 152, 363
AlwNI CAGNNNCTG 1 cut(s) 432
AoxI GGCC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 80, 82
BbsI GAAGAC 2 cut(s) 78, 569
BbvI GCAGC 1 cut(s) 391
BccI CCATC 4 cut(s) 248, 281, 566, 591
BceAI ACGGC 2 cut(s) 124, 423
BciT130I CCWGG 4 cut(s) 94, 168, 321, 627
BciVI GTATCC 1 cut(s) 599
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 471, 523
BfaI CTAG 1 cut(s) 491
BfoI RGCGCY 1 cut(s) 63
BfuI GTATCC 1 cut(s) 599
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 4 cut(s) 94, 168, 321, 627
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 437, 456
BmrFI CCNGG 4 cut(s) 94, 168, 321, 627
BmsI GCATC 2 cut(s) 172, 432
BpiI GAAGAC 2 cut(s) 78, 569
BpmI CTGGAG 3 cut(s) 429, 454, 495
BpuEI CTTGAG 1 cut(s) 247
BsaBI GATNNNNATC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 501
Bse118I RCCGGY 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 149
BseBI CCWGG 4 cut(s) 94, 168, 321, 627
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 3 cut(s) 259, 292, 602
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 352, 501
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 391
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 592
BsiSI CCGG 2 cut(s) 452, 495
BslI CCNNNNNNNGG 2 cut(s) 352, 501
BsmAI GTCTC 2 cut(s) 471, 523
BsmBI CGTCTC 1 cut(s) 523
BsnI GGCC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 471
Bsp143I GATC 3 cut(s) 144, 150, 355
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 1 cut(s) 519
BspLI GGNNCC 2 cut(s) 437, 456
BspPI GGATC 2 cut(s) 152, 363
BspTNI GGTCTC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 494
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 494
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 3 cut(s) 144, 150, 355
BssNAI GTATAC 1 cut(s) 260
Bst1107I GTATAC 1 cut(s) 260
Bst2UI CCWGG 4 cut(s) 94, 168, 321, 627
Bst4CI ACNGT 5 cut(s) 68, 308, 487, 590, 619
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 2 cut(s) 444, 527
BstF5I GGATG 3 cut(s) 259, 292, 602
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 3 cut(s) 147, 153, 358
BstMAI GTCTC 2 cut(s) 471, 523
BstMBI GATC 3 cut(s) 144, 150, 355
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 1 cut(s) 420
BstNI CCWGG 4 cut(s) 94, 168, 321, 627
BstSCI CCNGG 4 cut(s) 92, 166, 319, 625
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 2 cut(s) 78, 569
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
BstZ17I GTATAC 1 cut(s) 260
BsuI GTATCC 1 cut(s) 599
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 3 cut(s) 259, 292, 602
BtsIMutI CAGTG 3 cut(s) 217, 483, 615
Cac8I GCNNGC 1 cut(s) 496
CaiI CAGNNNCTG 1 cut(s) 432
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 494
Cfr13I GGNCC 1 cut(s) 170
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 1 cut(s) 488
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 2 cut(s) 56, 279
CviJI RGCY 7 cut(s) 172, 330, 369, 404, 414, 438, 494
CviKI_1 RGCY 7 cut(s) 172, 330, 369, 404, 414, 438, 494
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 2 cut(s) 444, 527
DpnI GATC 3 cut(s) 146, 152, 357
DpnII GATC 3 cut(s) 144, 150, 355
DraI TTTAAA 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 170
EcoRII CCWGG 4 cut(s) 92, 166, 319, 625
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 2 cut(s) 59, 282
FatI CATG 2 cut(s) 55, 278
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 3 cut(s) 266, 299, 609
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 61
GluI GCNGC 1 cut(s) 405
GsuI CTGGAG 3 cut(s) 429, 454, 495
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 1 cut(s) 172
HapII CCGG 2 cut(s) 452, 495
HhaI GCGC 1 cut(s) 62
Hin1II CATG 2 cut(s) 59, 282
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HinfI GANTC 2 cut(s) 429, 448
HpaII CCGG 2 cut(s) 452, 495
HphI GGTGA 2 cut(s) 80, 82
Hpy166II GTNNAC 2 cut(s) 233, 260
Hpy188I TCNGA 2 cut(s) 360, 428
Hpy188III TCNNGA 2 cut(s) 148, 433
Hpy8I GTNNAC 2 cut(s) 233, 260
HpyAV CCTTC 2 cut(s) 264, 346
HpyCH4III ACNGT 5 cut(s) 68, 308, 487, 590, 619
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 420
HpyF3I CTNAG 2 cut(s) 444, 527
Hsp92II CATG 2 cut(s) 59, 282
HspAI GCGC 1 cut(s) 60
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 3 cut(s) 144, 150, 355
LmnI GCTCC 2 cut(s) 435, 544
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 2 cut(s) 172, 432
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 1 cut(s) 491
MaeIII GTNAC 1 cut(s) 187
MalI GATC 3 cut(s) 146, 152, 357
MboI GATC 3 cut(s) 144, 150, 355
MboII GAAGA 3 cut(s) 83, 551, 569
MflI RGATCY 1 cut(s) 144
MluCI AATT 4 cut(s) 161, 177, 220, 312
MnlI CCTC 5 cut(s) 132, 183, 394, 404, 675
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 3 cut(s) 86, 132, 507
MspI CCGG 2 cut(s) 452, 495
MspR9I CCNGG 4 cut(s) 94, 168, 321, 627
MvaI CCWGG 4 cut(s) 94, 168, 321, 627
MwoI GCNNNNNNNGC 1 cut(s) 420
NaeI GCCGGC 1 cut(s) 496
NdeII GATC 3 cut(s) 144, 150, 355
NgoMIV GCCGGC 1 cut(s) 494
NlaIII CATG 2 cut(s) 59, 282
NlaIV GGNNCC 2 cut(s) 437, 456
NmuCI GTSAC 1 cut(s) 187
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 429, 448
PkrI GCNGC 1 cut(s) 406
Psp6I CCWGG 4 cut(s) 92, 166, 319, 625
PspGI CCWGG 4 cut(s) 92, 166, 319, 625
PspN4I GGNNCC 2 cut(s) 437, 456
PspPI GGNCC 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 432
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
SaqAI TTAA 3 cut(s) 86, 132, 507
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 3 cut(s) 144, 150, 355
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 4 cut(s) 94, 168, 321, 627
SetI ASST 7 cut(s) 95, 322, 332, 371, 406, 631, 635
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 2 cut(s) 172, 432
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 4 cut(s) 161, 177, 220, 312
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 4 cut(s) 92, 166, 319, 625
TaaI ACNGT 5 cut(s) 68, 308, 487, 590, 619
TasI AATT 4 cut(s) 161, 177, 220, 312
TatI WGTACW 1 cut(s) 487
TfiI GAWTC 2 cut(s) 429, 448
Tru1I TTAA 3 cut(s) 86, 132, 507
Tru9I TTAA 3 cut(s) 86, 132, 507
TscAI CASTG 3 cut(s) 217, 490, 622
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 44, 212
TspRI CASTG 3 cut(s) 217, 490, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.