pycom05g32580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009663
Physical Location & Seq
Reverse (-)
31931889 .. 31932350
462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32580.1

Sequence Viewer

Length: 462 bp
ATGGATAGAGAAAACCACGAAACGACTGTCGTTCATTCCTCCATAGCTCTACTGCAGGAAAGGTTTAGAGAGCTGCAGAGAGTGAAGGCAATGAGAGAGGAGAGAGAGGAGAGAGAGATTTCTAGAATGCTAGCTCTATCCTCTGACTCCGAACCAAAGCAGTTCACGGTGATGAATAGAAATCCCGCCATGCATAACGAGCCGACAAGGGCAAGGTTGTTTTTCCACCTTGATCATCTTGTCCACGGTCGTGGGACGTCGGCATCCGGAGCTTCTCTTTCCCTGTGGCCAACATTGCAGAGCAAGCATGAGGACTGTCCTCATGGGAAGACCGAGACACTGCTTTTGATCAACTTATGGCCAACTTTCAGAGACACCACCTCGTTAAAAACTTGTCCAAACAAATTTGAAGACTCGGATCCTAATTCTGACTCTGATGTAGATACTTCTCTTCATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.62

Weight (kDa)

5.97

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 1 cut(s) 186
AclWI GGATC 2 cut(s) 413, 426
AcoI YGGCCR 2 cut(s) 287, 359
AcsI RAATTY 1 cut(s) 404
AcyI GRCGYC 1 cut(s) 257
AgsI TTSAA 1 cut(s) 410
AleI CACNNNNGTG 1 cut(s) 249
AluBI AGCT 4 cut(s) 47, 73, 134, 272
AluI AGCT 4 cut(s) 47, 73, 134, 272
Alw26I GTCTC 2 cut(s) 329, 366
AlwI GGATC 2 cut(s) 413, 426
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 2 cut(s) 287, 359
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 404
ArsI GACNNNNNNTTYG 4 cut(s) 12, 44, 328, 360
AsuHPI GGTGA 1 cut(s) 181
AsuNHI GCTAGC 1 cut(s) 130
BalI TGGCCA 2 cut(s) 289, 361
BamHI GGATCC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 335, 417
BbvI GCAGC 1 cut(s) 60
BclI TGATCA 2 cut(s) 232, 348
BcoDI GTCTC 2 cut(s) 329, 366
BfaI CTAG 2 cut(s) 123, 131
BfmI CTRYAG 2 cut(s) 53, 74
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 420
BmsI GCATC 1 cut(s) 272
BmtI GCTAGC 1 cut(s) 134
BpiI GAAGAC 2 cut(s) 335, 417
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 266
Bse3DI GCAATG 2 cut(s) 96, 293
BseAI TCCGGA 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 1 cut(s) 263
BseMI GCAATG 2 cut(s) 96, 293
BseRI GAGGAG 2 cut(s) 113, 122
BseXI GCAGC 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 289, 361
BsiEI CGRYCG 1 cut(s) 250
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 329, 366
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 2 cut(s) 289, 361
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 3 cut(s) 232, 348, 418
BspACI CCGC 1 cut(s) 186
BspANI GGCC 2 cut(s) 289, 361
BspEI TCCGGA 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 420
BspMAI CTGCAG 2 cut(s) 57, 78
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 413, 426
BsrDI GCAATG 2 cut(s) 96, 293
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 3 cut(s) 232, 348, 418
BssNI GRCGYC 1 cut(s) 257
Bst4CI ACNGT 4 cut(s) 28, 169, 248, 317
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 132, 305
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 263
BstKTI GATC 3 cut(s) 235, 351, 421
BstMAI GTCTC 2 cut(s) 329, 366
BstMBI GATC 3 cut(s) 232, 348, 418
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 4 cut(s) 199, 269, 295, 304
BstSFI CTRYAG 2 cut(s) 53, 74
BstV1I GCAGC 1 cut(s) 60
BstV2I GAAGAC 2 cut(s) 335, 417
BstX2I RGATCY 1 cut(s) 418
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 2 cut(s) 289, 361
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 263
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 2 cut(s) 132, 305
CviAII CATG 3 cut(s) 190, 308, 323
CviJI RGCY 7 cut(s) 47, 73, 134, 202, 272, 289, 361
CviKI_1 RGCY 7 cut(s) 47, 73, 134, 202, 272, 289, 361
DpnI GATC 3 cut(s) 234, 350, 420
DpnII GATC 3 cut(s) 232, 348, 418
EaeI YGGCCR 2 cut(s) 287, 359
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 195
FaeI CATG 3 cut(s) 193, 311, 326
FaiI YATR 6 cut(s) 44, 191, 195, 309, 324, 358
FaqI GGGAC 1 cut(s) 268
FatI CATG 3 cut(s) 189, 307, 322
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 2 cut(s) 232, 348
Fnu4HI GCNGC 1 cut(s) 74
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 123, 131
GluI GCNGC 1 cut(s) 74
HaeIII GGCC 2 cut(s) 289, 361
HapII CCGG 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 3 cut(s) 193, 311, 326
HinfI GANTC 3 cut(s) 146, 413, 431
HpaII CCGG 1 cut(s) 267
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 165, 244
Hpy188I TCNGA 6 cut(s) 145, 151, 371, 418, 430, 436
Hpy188III TCNNGA 2 cut(s) 123, 267
Hpy8I GTNNAC 2 cut(s) 165, 244
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 4 cut(s) 28, 169, 248, 317
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 4 cut(s) 55, 76, 193, 298
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 269, 295, 304
HpySE526I ACGT 1 cut(s) 257
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 3 cut(s) 193, 311, 326
Kpn2I TCCGGA 1 cut(s) 266
Ksp22I TGATCA 2 cut(s) 232, 348
Kzo9I GATC 3 cut(s) 232, 348, 418
LmnI GCTCC 1 cut(s) 269
LpnPI CCDG 3 cut(s) 41, 280, 296
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 1 cut(s) 272
MaeI CTAG 2 cut(s) 123, 131
MaeII ACGT 1 cut(s) 257
MalI GATC 3 cut(s) 234, 350, 420
MboI GATC 3 cut(s) 232, 348, 418
MboII GAAGA 3 cut(s) 340, 422, 443
MflI RGATCY 1 cut(s) 418
MlsI TGGCCA 2 cut(s) 289, 361
MluCI AATT 2 cut(s) 404, 424
MluNI TGGCCA 2 cut(s) 289, 361
MlyI GAGTC 3 cut(s) 140, 407, 425
MnlI CCTC 7 cut(s) 49, 91, 100, 151, 304, 330, 391
Mox20I TGGCCA 2 cut(s) 289, 361
Mph1103I ATGCAT 1 cut(s) 195
MroI TCCGGA 1 cut(s) 266
MscI TGGCCA 2 cut(s) 289, 361
MseI TTAA 1 cut(s) 386
MslI CAYNNNNRTG 2 cut(s) 170, 249
Msp20I TGGCCA 2 cut(s) 289, 361
MspI CCGG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 132
MwoI GCNNNNNNNGC 4 cut(s) 199, 269, 295, 304
NdeII GATC 3 cut(s) 232, 348, 418
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 3 cut(s) 193, 311, 326
NlaIV GGNNCC 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 195
OliI CACNNNNGTG 1 cut(s) 249
PctI GAATGC 1 cut(s) 132
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 3 cut(s) 140, 407, 425
PpsI GAGTC 3 cut(s) 140, 407, 425
PspN4I GGNNCC 1 cut(s) 420
PstI CTGCAG 2 cut(s) 57, 78
PsuI RGATCY 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 170, 249
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 3 cut(s) 232, 348, 418
SchI GAGTC 3 cut(s) 140, 407, 425
SetI ASST 9 cut(s) 49, 65, 75, 136, 218, 231, 260, 274, 383
SfaNI GCATC 1 cut(s) 272
SfcI CTRYAG 2 cut(s) 53, 74
SmiMI CAYNNNNRTG 2 cut(s) 170, 249
Sse9I AATT 2 cut(s) 404, 424
SsiI CCGC 1 cut(s) 186
SspMI CTAG 2 cut(s) 123, 131
TaaI ACNGT 4 cut(s) 28, 169, 248, 317
TaiI ACGT 1 cut(s) 260
TaqII GACCGA 1 cut(s) 347
TasI AATT 2 cut(s) 404, 424
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 1 cut(s) 345
TseI GCWGC 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 23, 188, 443
TspRI CASTG 1 cut(s) 345
XapI RAATTY 1 cut(s) 404
XbaI TCTAGA 1 cut(s) 122
XspI CTAG 2 cut(s) 123, 131
ZraI GACGTC 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.